Exploring Valid Reference Genes For Quantitative Real-Time Pcr Analysis In Plutella Xylostella (Lepidoptera: Plutellidae), Wei Fu, Wen Xie, Zhuo Zhang, Shaoli Wang, Qingjun Wu, Yong Liu, Xiaomao Zhou, Xuguo Zhou, Youjun Zhang

Entomology Faculty Publications

Quantitative real-time PCR (qRT-PCR), a primary tool in gene expression analysis, requires an appropriate normalization strategy to control for variation among samples. The best option is to compare the mRNA level of a target gene with that of reference gene(s) whose expression level is stable across various experimental conditions. In this study, expression profiles of eight candidate reference genes from the diamondback moth, Plutella xylostella, were evaluated under diverse experimental conditions. RefFinder, a web-based analysis tool, integrates four major computational programs including geNorm, Normfinder, BestKeeper, and the comparative ΔCt method to comprehensively rank the tested candidate …

The Problem With Adhd: Researchers' Constructions And Parents' Accounts, Bora Pajo, David Cohen Dr.

All Faculty and Staff Scholarship

An enduring controversy over the nature of ADHD complicates parents’ decisions regarding children likely to be diagnosed with the condition. Using a fallibilist perspective, this review examines how researchers construe ADHD and acknowledge the controversy. From a systematic literature search of empirical reports using parents of ADHD-diagnosed children as primary informants, 36 reports published between 1996 and 2008 (corresponding to 30 studies) were selected. Data on the studies’ characteristics and methodologies, definitions of ADHD, and extent of the acknowledgment of the ADHD controversy were extracted, as were data on a wide range of parental concerns and experiences. Researchers in 27 …

Multidisciplinary Treatment For Conversion Disorder In An 8 Year Old Girl, Jen Wildpret, Sebastian Kaplan

Jefferson Journal of Psychiatry

We present the case of an 8 year-old-girl with a 3-week history of visual conversion, inability to walk, intermittent urinary incontinence, and non-epileptic seizures. Although she had a history of shaken baby syndrome that resulted in hydrocephalus requiring bilateral ventriculoperitoneal (VP) shunt placement at 3 months of age, this current episode could not be attributed to any organic causes and hence she was diagnosed with conversion disorder with mixed presentation. In the absence of published recommendations for treatment of childhood conversion disorder, our multidisciplinary treatment team designed a biopsychosocial approach to improve her symptoms, strengthen family dynamics and provide her …

Treatment Of The Mentally Ill In The Pre-Moral And Moral Era: A Brief Report, Michael A. Carron, Hanna Saad

Jefferson Journal of Psychiatry

Throughout the ages, treatment of the mentally ill has evolved with distinct periods of progression, stagnation and regression. These differences in time mirrored how society understood mental illness and the mentally ill person on both a biological and interpersonal level. At various points in history, treatment of the mentally ill included cruel and inhumane acts, while at other times, consisted of compassionate and benevolent care. What follows is a brief comparison of how mental illness was conceptualized and how persons with mental illness were treated in the pre-moral and moral eras of medicine. The pre-moral era is from the end …

The Heterogeneity And Metastability Of Multipotent Hematopoietic Cells, Wendy W. Weston

Open Access Dissertations

Increasing numbers of studies are reporting phenotypic and functional heterogeneity within a variety of stem cell types as well as the existence of inter-convertible subsets within hematopoietic stem cells (HSCs), suggesting that these cells are alternating between several metastable states. The extent of heterogeneity and metastability in HSCs remains to be explored in depth. The metastability of HSCs is most likely positively and negatively regulated by currently unknown cell intrinsic and extrinsic mechanisms emanating from HSC niches. But the regulation of HSC metastability by bone marrow (BM) niche cells is not currently understood. Numerous studies have shown that phenotypic, functional …

Potentiating Anti-Tumor Immunity Using Aptamer-Sirna Conjugates, Randall J. Brenneman

Open Access Dissertations

The host immune system plays an important role in the development and control of cancer, yet cancer cells often elaborate mechanisms to evade this regulation. Transforming growth factor beta (TGFβ) is a pleiotropic cytokine often overexpressed by cancers due to TGFβ’s growth-promoting effect on tumor cells and suppressive function on multiple cell types of the innate and adaptive immune systems. Genetic ablation of TGFβ signaling induces significant and toxic immune responses against host tissues, but when signaling is selectively ablated in cells of the adaptive immune system, such as CD8+ T cells, they become capable of mediating protection against various …

The Bermuda Triangle: Lost In Transition, Kevin S. Koett, C. Thomas Potter Ii

Morehead State Theses and Dissertations

A capstone submitted in partial fulfillment of the requirements for the degree of Doctor of Education in the College of Education at Morehead State University by Kevin S. Koett and C. Thomas Potter II on July 19, 2013.

Electron Transfer By Excited Benzoquinone Anions: Slow Rates For Two-Electron Transitions, Matibur Zamadar, Andrew R. Cook, Anna Lewandowska-Andralojc, Richard Holroyd, Yan Jiang, Jin Bikalis, John R. Miller

Faculty Publications

Electron transfer (ET) rate constants from the lowest excited state of the radical anion of benzoquinone, BQ−•*, were measured in THF solution. Rate constants for bimolecular electron transfer reactions typically reach the diffusion-controlled limit when the free-energy change, ΔG°, reaches −0.3 eV. The rate constants for ET from BQ−•* are one-to-two decades smaller at this energy and do not reach the diffusion-controlled limit until −ΔG° is 1.5−2.0 eV. The rates are so slow probably because a second electron must also undergo a transition to make use of the energy of the excited state. Similarly, ET, from solvated electrons to neutral …

Dual-Role Appointment Enhances Uab Health System, Uahsf Operations, Staff

Browse All News

No abstract provided.

Today - July 19, 2013, Loma Linda University Health

TODAY

Inside this issue:

-- Legislators visit Loma Linda University Health
-- LLU neuroscientist awarded $1.9 million NIH grant to reduce stroke damage
-- Six-year-old philanthropist gathers 33,000 stickers for kids at LLU Children's Hospital
-- Healthy competition allows employees to step up their wellness
-- Lauren Abadie's unexpected response to Type 1 diabetes
-- Care received at La Loma Luz Adventist Hospital leads dental hygiene student to choose LLU
-- Housekeeping staff gets top training at Medical Simulation Center
-- 'Loma Linda 360' garners five Emmy Awards
-- Pharmacy student selected for residency at VA
-- LLU fosters higher education …

The Impact Of China On The International Dry Bulk Shipping Market, Hailun Zhang

World Maritime University Dissertations

No abstract provided.

Developing Thyronamine Analog Pharmaceuticals Targeting Taar1 To Treat Methamphetamine Addiction, Troy Andrew Wahl

Dissertations and Theses

As a part of the overall program in the Grandy laboratory at Oregon Health & Science University (OHSU), studying the underlying chemical biology of methamphetamine (Meth) addiction, this dissertation reports on the development of six new thyronamine analogs which were synthesized and assayed against trace amine associated receptor 1 (TAAR1), giving preliminary results consistent with the analogs being inverse agonists. Due to highly variable TAAR1 expression levels in the assays, based on inter-assay response to control Meth stimulation as well as other possible factors, kinetic models were developed to qualitatively explain the assay results. The models set approximate limits on …

Holocarboxylase Synthetase Represses Long Terminal Repeats Through Epigenetic Synergies Between Biotin And Methyl Donors, And Activates Immune Response By Catalyzing Biotinylation Of Heat Shock Protein 72, Jing Xue

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Holocarboxylase synthetase (HLCS) is the sole human protein biotin ligase in the human proteome. Other than its role as a coenzyme to the five carboxylases in intermediary metabolism, HLCS and biotin are involved in epigenetic gene regulation and immune response. Previously, it was shown that (1) HLCS physically interacts with histone methyl transferase EHMT-1 which creates histone H3 lysine 9 methylation (H3K9me) marks and biotinylates lysine (K) 161 in EHMT-1, thereby strenghtening the HLCS/EHMT-1 interaction; (2) biotin depletion and HLCS knockdown cause a loss of H3K9me marks leading to de-repression of long terminal repeats (LTRs) and chromosomal abnormalities; and (3) …

The Effects Of A Model Androgen 5a-Dihydrotestosterone On Mummichog (Fundulus Heteroclitus) Reproduction Under Different Salinities, Chelsea O'Connor Blatchley

Master's Theses

Abstract

Endocrine disrupting substances (EDSs) have the potential to disrupt sensitive hormone pathways, including those involved in development and reproduction. Both fresh and estuarine water bodies receive inputs of EDSs from a variety of sources, including sewage effluent, industrial effluent, and agricultural runoff. Based on limited available literature, freshwater species appear to respond to lower levels of EDSs than estuarine or marine species. To address this potential difference, a short-term reproductive bioassay was carried out under low and high salinity conditions using mummichog (Fundulus heteroclitus), a euryhaline species that is native to the east coast of North America. …

Differential Impacts Of Juvenile Hormone, Soldier Head Extract And Alternate Caste Phenotypes On Host And Symbiont Gene Expression In The Gut Of The Termite Reticulitermes Flavipes., Ruchira Sen, Rhitoban Raychoudhury, Yunpeng Cai, Yijun Sun, Verena-Ulrike Lietze, Drion G. Boucias, Ruchira E. Sen

Department of Entomology Faculty Publications

Background

Termites are highly eusocial insects and show a division of labor whereby morphologically distinct individuals specialize in distinct tasks. In the lower termite Reticulitermes flavipes(Rhinotermitidae), non-reproducing individuals form the worker and soldier castes, which specialize in helping (e.g., brood care, cleaning, foraging) and defense behaviors, respectively. Workers are totipotent juveniles that can either undergo status quo molts or develop into soldiers or neotenic reproductives. This caste differentiation can be regulated by juvenile hormone (JH) and primer pheromones contained in soldier head extracts (SHE). Here we offered worker termites a cellulose diet treated with JH or SHE …

Genetic Dissection Of Quantitative Trait Loci For Substances Of Abuse, Jo Lynne Harenza

Theses and Dissertations

It has been reported that an individual’s initial level of response to a drug might be predictive of his or her future risk of becoming dependent, thus basal gene expression profiles underlying those drug responses may be informative for both predicting addiction susceptibility and determining targets for intervention. This dissertation research aims to elucidate genetic risk factors underlying acute alcohol and nicotine dependence phenotypes using mouse genetic models of addiction. Phenotyping, brain region-specific mRNA expression profiling, and genetic mapping of a recombinant inbred panel of over 25 mouse strains were performed in order to identify quantitative trait loci (QTL) harboring …

From Slurs To Science, Racism To Revisionism: White Nationalist Rhetors And Legitimation In The Stormfront Community, Claire Davies Rhodes

Electronic Theses and Dissertations Archive

While on the surface mainstream discussions about race appear to encompass the values and ideologies associated with egalitarianism, subtle and at times not so subtle discussions continue to disparage groups and individuals who have been historically associated with the minority. The growing existence of hate groups illustrates that arguments for these extreme ideologies are not only present but for some individuals are gaining in acceptance. For this reason, I perform a critical discourse analysis of the language of one of the most popular and prolific White Nationalist groups on the Internet, Stormfront. In particular, I examine how scientific and revisionist …

Computer Simulations Of Nanorods Interaction With Lipid Membrane, Niraj Shrestha

Electronic Theses and Dissertations Archive

Analyzing the interaction between the lipid membranes and nanoparticles helps to determine beneficial and harmful effects of nanoparticles on cells. Since the point of entry of cells is their plasma membrane, interactions between nanorods and lipid membranes were studied using molecular dynamics simulations. The effect of nanorods on membrane morphology was investigated systematically as a function of nanorods interaction with the lipid membrane, their surface coverage and number surface density. Our results indicate that when adsorbed on the membrane, the nanorods may cluster into chains and may lead to strong membrane deformations due to curvature driven interaction between nanorods. At …

Research On Optimization Purchasing Logistics Of Civil Aviation Manufacturing Company Based No Supply Chain Management, Zhongxi Zhang

World Maritime University Dissertations

No abstract provided.

Chinese Vessel Pollution Prevention In The Era Of The Green Economy, Zhilin Zheng

World Maritime University Dissertations

No abstract provided.

Differential Regulation Of White-Opaque Switching By Individual Subunits Of Candida Albicans Mediator, Anda Zhang, Zhongle Liu, Lawrence C. Myers

Dartmouth Scholarship

The multisubunit eukaryotic Mediator complex integrates diverse positive and negative gene regulatory signals and transmits them to the core transcription machinery. Mutations in individual subunits within the complex can lead to decreased or increased transcription of certain subsets of genes, which are highly specific to the mutated subunit. Recent studies suggest a role for Mediator in epigenetic silencing. Using white-opaque morphological switching in Candida albicans as a model, we have shown that Mediator is required for the stability of both the epigenetic silenced (white) and active (opaque) states of the bistable transcription circuit driven by the master regulator Wor1. Individual …

South Carolina Mayflies (Insecta: Ephemeroptera) Of Conservation Concern, Luke M. Jacobus

Journal of the South Carolina Academy of Science

Abstract: Nine mayfly species (Insecta: Ephemeroptera) that may be of conservation concern in South Carolina are discussed. Three such species associated with sand-bottomed streams are Acanthametropus pecatonica (Burks, 1953), Dolania americana Edmunds & Traver, 1959 and Homeoneuria dolani Edmunds, Berner & Traver, 1958. Three species of potential concern are associated with Hornleaf Riverweed (Podostemum ceratophyllum Michaux, 1803), and they include Barbaetis benfieldi Kennedy, 1985, Heterocloeon berneri (Muller-Liebenau, 1974) and Tsalia berneri (Allen & Edmunds, 1958). Mayflies of slow or stagnant waters that may be of conservation concern in South Carolina include Arthroplea bipunctata (McDunnough, 1924), Macaffertium lenati (McCafferty, 1990) …

Ernest Everett Just, Phd: Pioneer In Ecological Developmental (Eco-Devo) Biology, Katelyn M. Williams, Bryan A. Wilson, Wendi G. O'Connor, Monte S. Willis

Journal of the South Carolina Academy of Science

Ernest Everett Just, a pioneering American biologist, discovered the fundamental role of the environment in the development of embryos. His work led to the creation of the area of biology known as ecological developmental (Eco-Devo) biology. However, both his work and the context of his scientific contributions are not widely known. His work covered a diversity of fields of biology, including marine biology, cytology, and parthogenesis (asexual reproduction where growth and development of embryos occur without fertilization). His findings provided important concepts in developmental biology that are used to this day. Specifically, he demonstrated the importance of the cellular cytoplasm …

Computer Vision Based, Non-Contacting Deformation And Shape Measurements: A Revolution In Progress, Michael A. Sutton

Journal of the South Carolina Academy of Science

The rapid expansion of computer technology in the past thirty years has impacted society in innumerable ways. Of interest for this discussion is the breath-taking change in measurement sciences that has occurred as the digital age dawned and has now become part of our daily lives. In particular, the effect on image-based, non-contacting measurements will be described and the continuing revolutionary impact that the resulting technology has engendered will be reviewed. Additionally, the unprecedented expansion of capabilities will be highlighted by presenting a set of illustrative example studies.

Table Of Contents, Michele Harmon

Journal of the South Carolina Academy of Science

No abstract provided.

Randomized Controlled Trial Of A Behavioral Weight Loss Intervention For Primary Prevention Of Renal Decline In Type 2 Diabetics, Ashley E. Moncrieft

Open Access Dissertations

Reduced glomerular filtration rate and increased albuminuria often develop in Type 2 Diabetes Mellitus (T2DM) and are predictive of chronic kidney and cardiovascular disease. Risk for renal disease in T2DM has been associated with cardiometabolic risk factors including, hypertension, dyslipidemia, and hyperglycemia. Weight loss is associated with improved outcomes in T2DM patients with existing renal disease and may also reduce risk in patients without renal disease via reduction of cardiometabolic risk factors. The aims of this study were (1) to examine the effects of a behavioral weight loss intervention on renal outcomes in T2DM patients without evidence of renal disease, …

Reviving Bay Life, Jill Rodrigues

Featured News Story

With support via the R.I. Science and Technology Advisory Council, MNS faculty members partner on projects to improve state's marine ecosystem.

Blog Post, Fish And Salsa!, 'Burg Blogs, July 18, 2013, Lisa Lugo

USF St. Petersburg Campus News and Press Releases

No abstract provided.

Isso Weekly Newsletter, July 18, 2013, University Of Northern Iowa. International Students And Scholars Office.

ISSO Weekly Newsletter

Inside This Issue:

-- ISSO Office Has Moved
-- Archived Newsletters on ISSO website
-- Fraud Alert - International Student Scam
-- BBQ'Loo & Blues Too July 19-20
-- Bicycles Available
-- Furniture Available
-- Waterloo Bucks Baseball July 18
-- ISSO Summer Office Hours
-- Things to Do in the Summer
-- Reminder to like our Facebook page
-- ISSO - Enhancing International Experiences

State V. Marks Appellant's Brief Dckt. 39684

Idaho Supreme Court Records & Briefs, All

No abstract provided.