Digital Commons Network^™

Modeling Nitrogen Transformations In A Pilot Scale Marine Integrated Aquaculture System, Brian Mccarthy

USF Tampa Graduate Theses and Dissertations

Integrated aquaculture systems (IAS) are a type of recirculating aquaculture systems (RAS) where the wastewater is treated and returned to the fish tanks. The important difference between the two is that in an IAS, wastes from the aquaculture component are recovered as fertilizer to produce an agricultural product whereas in an RAS, waste organics, nutrients and solids are treated and discharged. A pilot marine IAS at Mote Aquaculture Research Park in Sarasota, FL was studied for this project. Water quality monitoring, measurements of fish health and growth rates of fish and plants were performed over a two-year period to determine …

The Experiences Of Mothers Of Children With Autism In Jamaica: An Exploratory Study Of Their Journey, Angela R. Mann

USF Tampa Graduate Theses and Dissertations

Autism Spectrum Disorders (ASDs), also referred to as Pervasive Developmental Disorders (PDDs), are characterized by deficits in verbal and nonverbal communication, social interaction, and repetitive or restricted interests and behaviors. To date, much of the literature on ASDs has been conducted from a western perspective, although interest in ASDs from a global perspective has dramatically increased in recent years. Over the last decade, there have been numerous conceptual papers attempting to explain how autism might be experienced in other parts of the world. However, in actuality, little research has been conducted in this area, and further exploration of the experiences …

Understanding Amphibian Decline: The Role Of Pesticides And The Pathogenic Chytrid Fungus On Amphibians And Aquatic Communities, Taegan A. Mcmahon

USF Tampa Graduate Theses and Dissertations

Amphibians are the most threatened taxon on the planet. Declines have been associated with over-exploitation, habitat loss, pollution, and pathogenic diseases, but of these factors, pollution and disease have been relatively under-studied. Here, I investigated: 1) the impacts of commonly used pesticides on aquatic communities, 2) the effect of these pesticides on amphibian susceptibility to the pathogenic chytrid fungus, Batrachochytrium dendrobatidis (Bd), and 3) whether there are non-amphibian hosts of Bd and 4) how to best quantify the survival of Bd through ontogeny of the host.

In my first research chapter, I quantified the effects of environmentally relevant concentrations of …

Structure And Function Of Pinniped Vibrissae, Christin Taylor Murphy

USF Tampa Graduate Theses and Dissertations

The vibrissal system of pinnipeds relies on sturdy, specialized vibrissae and supporting neural architecture apparently designed for the reception of waterborne disturbances. Although it is known that pinnipeds can use their vibrissae for fine-scale tactile discrimination and hydrodynamic detection, many aspects of vibrissal function remain poorly understood. The present work examined the adaptive significance of vibrissal structure, the sensitivity of the vibrissal system, and the signals received by this system. All of these points were considered with respect to their function in hydrodynamic reception. Four methods of study: laser vibrometry, computed tomography (CT) scanning, psychophysical testing and animal-borne tagging were …

Quantifying The Ergonomic Impact On Healthcare Workers Using A Needle-Free Injector Device, Humberto Jose Olivero Lara

USF Tampa Graduate Theses and Dissertations

Background: Jet injectors are advantageous over needle injectors by eliminating sharps hazards. The Government Accountability Office estimates 29% preventable sharp injuries with an estimated direct cost of more than $500 million out of the CDC's reported incidence of 385,000 needle stick injuries per year among US hospital healthcare workers. Yet the forces required to set and trigger devices using spring mechanisms for medication delivery have not been explored. This laboratory experiment measured forces exerted by healthcare workers (HCWs) using a particular jet injector approved by FDA in 2011.

Objectives: In order to quantify the ergonomic impact on HCWs using a …

Fertile Ground For A Social Movement: Social Capital In Direct Agriculture Marketing, Elizabeth A. Murray

USF Tampa Graduate Theses and Dissertations

Building from existing literature on anthropology of food, political economy of food and consumption, and social movement theory, I examine the direct agriculture network of Tampa Bay Florida through a mixed-method ethnography. The research consisted of one year of field-work, with 6 months and over 100 hours of active participant observation, open-ended interviews with eight local producers, and short surveys with 100 market patrons. This thesis is an analysis of the results of this rigorous qualitative and quantitative work and, perhaps more importantly, an account of my own personal struggles in joining the direct agriculture network and my ultimate commitment …

Interrogation Of Epor Fidelity In Myelodysplastic Syndrome Hematopoiesis And Stabilization By The Immunomodulatory Agent, Lenalidomide, Kathy Lynn Mcgraw

USF Tampa Graduate Theses and Dissertations

Myelodysplastic syndromes (MDS) include a spectrum of stem cell malignancies characterized by ineffective hematopoiesis and predisposition to acute myeloid leukemia (AML) transformation. Patients are predominantly older (greater than 60 years old), with progressive cytopenias resulting from ineffective and cytologically dysplastic hematopoiesis. MDS subtypes are classified by morphologic features and bone marrow blast percentage, as well as cytogenetic pattern, as is the case for deletion 5q MDS. Interstitial deletion of the long arm of chromosome 5, del(5q), is the most common chromosomal abnormality in patients with MDS, and the 5q- syndrome, represents a distinct subset of del(5q) MDS characterized by an …

Impacts Of The Anomalous Mississippi River Discharge And Diversions On Phytoplankton Blooming In Northeastern Gulf Of Mexico, Brendan O'Connor

USF Tampa Graduate Theses and Dissertations

On April 20, 2010 a tragic explosion aboard the Deepwater Horizon (DWH) drilling rig marked the beginning of one of the worst environmental disasters in history. For 87 days oil and gas were released into the Gulf of Mexico. In August 2010, anomalous phytoplankton activity was identified in the Northeastern Gulf of Mexico, using the Fluorescence Line Height (FLH) ocean color product. The FLH anomaly was bound by approximately 30-28 degrees North and 90 and 86 degrees West and there was a suggestion that this anomaly may have occurred due to the presence of oil. This study was designed to …

Applications Of Agent Based Approaches In Business: A Three Essay Dissertation, Shankar Prawesh

USF Tampa Graduate Theses and Dissertations

The goal of this dissertation is to investigate the enabling role that agent based simulation plays in business and policy. The aforementioned issue has been addressed in this dissertation through three distinct, but related essays. The first essay is a literature review of different research applications of agent based simulation in various business disciplines, such as finance, economics, information systems, management, marketing and accounting. Various agent based simulation tools to develop computational models are discussed. The second essay uses an agent-based simulation approach to study important properties of the widely used most popular news recommender systems (NRS). This essay highlights …

Statistical Analysis And Modeling Of Brain Tumor Data: Histology And Regional Effects, Keshav Prasad Pokhrel

USF Tampa Graduate Theses and Dissertations

Comprehensive statistical models for non-normally distributed cancerous tumor sizes are

of prime importance in epidemiological studies, whereas a long term forecasting models

can facilitate in reducing complications and uncertainties of medical progress. The statistical

forecasting models are critical for a better understanding of the disease and supply

appropriate treatments. In addition such a model can be used for the allocations of budgets,

planning, control and evaluations of ongoing efforts of prevention and early detection of

the diseases.

In the present study, we investigate the effects of age, demography, and race on primary

brain tumor sizes using quantile regression methods to …

Expectations Of Nursing Home Use, Psychosocial Characteristics And Race/Ethnicity: The Latino/A Case, Heidi Ross

USF Tampa Graduate Theses and Dissertations

This study used data from the 2008 wave of the Health and Retirement Study to examine variations in relationships among selected psychosocial characteristics, race/ethnicity and expectations of nursing home utilization in the United States, with a particular focus on Latino/a subgroups. This study sought to test a modified version of the Andersen and Newman model of health service utilization. Findings revealed that expectations of nursing home utilization remained lower among Latino/as than in the Non-Latino White sub-groups, even when levels of need, enabling, and predisposing factors were controlled for. However, for Mexican Origin respondents (who are often arbitrarily combined with …

Essays In Happiness Economics, Boris Nikolaev

USF Tampa Graduate Theses and Dissertations

The goal of this dissertation is to contribute to the new field of happiness economics which over the past several decades has substantially enhanced our understanding of cognitive judgment, human behavior, and the nature of happiness. Chapter 1 starts with a discussion of the subjective approach to measuring well-being and lays the foundation for the empirical work that follows in chapters 2 and 3. This approach has a strong appeal because ancient and modern cultures, and a long tradition in philosophy, view achieving happiness as the ultimate goal of human existence. It also recognizes that humans are the best judges …

An Investigation Of The Factors Leading To Invasion Success Of Non-Native Plants Using A System Of Native, Introduced Non-Invasive, And Invasive Eugenia Congeners In Florida, Kerry Bohl

USF Tampa Graduate Theses and Dissertations

The overwhelming majority of plant species introduced into a new range never become invasive. Consequently, identification of factors allowing the small fraction of successful invaders to naturalize, increase in abundance, and displace resident species continues to be a key area of research in invasion biology. Of the considerable number of hypotheses that have been proposed to resolve why some plant species become noxious pests, the enemy release hypothesis (ERH) is one of the most commonly cited. The ERH maintains that invasive plants succeed in a new range because they are no longer regulated by their coevolved natural enemies, and this …

Structural Basis For Ternary Complex Formation Between Tau, Hsp90, And Fkbp51, Alexander Steven Barrett

USF Tampa Graduate Theses and Dissertations

The accumulation of the microtubule associated protein tau has been implicated in several neurological disorders; however, its interaction with chaperones along its normal degradation pathway remains largely uncharacterized at single residue resolution. In this study, nuclear magnetic resonance (NMR) spectroscopy was used to probe the interaction between tau, the molecular chaperone Hsp90, and the immunophilin FKBP51. Resonance intensity changes were observed for specific residues in the heteronuclear single quantum coherence (HSQC) spectra of 15N-labeled tau in the presence of Hsp90 and/or FKBP51. Analysis of the HSQC spectra identified the two hydrophobic hexapeptide motifs located at residues V275 - K280 and …

Environmental Effects On The Biomechanics And Motor Physiology Of Elastically Powered Movements In Chameleons, Christopher Van Anderson

USF Tampa Graduate Theses and Dissertations

Environmental temperature exhibits profound effects on the activity and ecology of ectotherms through its impact on muscle contractile physiology. While the performance of locomotor behaviors powered by muscle contraction directly decreases by at least 33% over a 10°C drop in body temperature, chameleons are known to feed, presumably with high performance, at body temperatures where sympatric lizard species remain inactive. I propose that ballistic movements that are powered by the recoil of preloaded elastic and collagenous tissues are less thermally dependent than movements that rely on direct muscular power. Despite the reduced thermal sensitivity of the elastic-recoil powered movement, I …

Evaluation Of A Pilot Land-Based Marine Integrated Aquaculture System, Suzanne Boxman

USF Tampa Graduate Theses and Dissertations

Recirculating aquaculture systems (RAS) produce aquaculture products on land with minimal discharge of waste products and minimal water loss. High costs associated with waste treatment for RAS have triggered the growth of integrated aquaculture systems (IAS) which incorporate macrophytes (aquatic plants) into the treatment train. The objective of this research was to examine a pilot scale inland marine IAS with three different methods for solids treatment: a sand filter followed by a plant bed, only a plant bed, and geotextile bags. Florida Pompano (Trachinotus carolinus) were grown along with Smooth Cordgrass (Spartina alterniflora), Black Needle Rush …

Partial Characterization Of Pf13_0027: A Putative Phosphatase Of Plasmodium Falciparum, Christopher Campbell

USF Tampa Graduate Theses and Dissertations

Signal transduction and stage-specific gene expression are essential components of Plasmodium falciparum development. In this study, the putative phosphatase PF13_0027 is investigated as a critical component of intraerythrocytic development contributing to maturation of the late trophozoite. This putative phosphatase was identified during the course of a large-scale insertional mutagenesis project by insertion of the piggyBac (pB) element, containing a human dihydrofolate reductase (hDHFR) drug selection cassette into the open reading frame (ORF) preventing expression and attenuating parasite development. PF13_0027 codes for a protein with a rhodanese (RHD) and dual specificity phosphatase (DUSP) in a tandem arrangement typically identified with mitogen-activated …

"They Come, But They Don't Spend As Much Money": Livelihoods, Dietary Diversity, Food Security, And Nutritional Status In Two Roatan Communities In The Wake Of Global Crises In Food Prices And Finance, Racine Marcus Brown

USF Tampa Graduate Theses and Dissertations

ABSTRACT

This dissertation explores the associations between recent global crises in staple food prices and finance and the following aspects of life in two communities on the island of Roatàn, Islas de La Bahia (Bay Islands), Honduras: household livelihoods; food commoditization; dietary diversity; food security; and nutritional status. The aims of this study are: ) assess the geographic and economic source(s) of foods consumed by two different communities on Roatàn; b) discover how the most recent economic and food crises have affected foodways and nutrition on Roatàn; c) assess how these crises have affected economic growth of the tourism sector …

The Role Of Molecular Chaperones In The Etiology And Treatment Of Psychiatric Diseases In The Elderly, John Clarence O'Leary

USF Tampa Graduate Theses and Dissertations

The elderly are at increased risk for developing psychiatric diseases, which include Alzheimer's disease, depression, anxiety and suicide. The probability of multiple disease comorbidity is also increased in the elderly. At the cellular level, the loss of protein homeostasis is often at the root of disease emergence, and thus the scientific community is searching for ways to help maintain this balance. A vast group of proteins that are paramount to balancing and counterbalancing protein levels is the molecular chaperone protein group, which has evolved a tremendous variety of functions in the cell. They aid in protein trafficking, folding, receptor signaling, …

The Elimination Of Blindness: An Ethnographic Exploration Of The Fight Against Trachoma In Niger, Kelley Cosby Sams

USF Tampa Graduate Theses and Dissertations

The goal of this study is to explore specialized and popular cultural models of trachoma, and the interaction between the trachoma elimination program and its target audience in one trachoma hyper-endemic intervention community. Eighty four million people worldwide, mainly children, are infected with active bacterial trachoma. For some, this will lead to painful and progressive corneal opacity and eventual blindness. The disease is most commonly spread by person-to-person contact or by flies, and affects very specific populations living in resource-poor areas such as rural Niger, which has one of the highest prevalence rates worldwide.

The World Health Organization formed an …

The Effect Of Rate Change On The Relative Timing Of Speakers With Multiple Sclerosis, Brandlynn N. Reister

USF Tampa Graduate Theses and Dissertations

Relative timing ratios are a useful measure for determining the temporal regularities of speech. The timing intervals that make up these ratios are thought to be important when creating the motor plan for an utterance (Weismer & Fennell, 1985). In fact, these ratios have been shown to be remarkably stable, even when speakers deliberately increase their rate (Tuller & Kelso, 1984; Weismer & Fennell, 1985). The constancy of these ratios also has been demonstrated in speakers with known speech timing disturbances, like the dysarthrias associated with Parkinson's and Huntington's disease (Goberman & McMillan; Ludlow, Connor, & Bassich, 1987; Weismer & …

Beyond The Door: Disability And The Sibling Experience, Morgan Violeta Sanchez Taylor

USF Tampa Graduate Theses and Dissertations

This thesis explores the experiences of adult siblings of individuals with impairments. It expands on the existing literature by exploring the complexity of the sibling experience of disability while moving beyond the concepts of burden and maladjustment that have characterized much of the previous literature. In addition, it expands upon and extends to the sibling experience an emerging view of disability by examining the ways in which themes identified in sibling narratives cross lines between the Medical and Social Models of Disability. Building on work by Mark Priestly and Tom Shakespeare, I call this emerging view the Interactional Model of …

Negotiating Race-Related Tensions: How White Educational Leaders Recognize, Confront, And Dialogue About Race And Racism, Amy Jo Samuels

USF Tampa Graduate Theses and Dissertations

Despite exposure of educational disparities for students of color, as well as the notion that educational training rarely discusses race and racism, there continues to be a lack of discourse on race, racism, and anti-racism in educational leadership. Subsequently, it is important to challenge deficit thinking and encourage further examination of the deeply-rooted foundation of oppression. The study explored personal narratives of white educational leaders who oppose racial inequity to heighten awareness about conceptualizations of race, racism, and anti-racism. The research involved interviewing educational leaders in three groups: 1) aspiring, 2) currently-practicing, and 3) recently-retired. Eight participants were selected to …

Mass Spectrometry-Based Methods For The Detection And Characterization Of Protein-Tyrosine Nitration, Kent W. Seeley

USF Tampa Graduate Theses and Dissertations

Protein tyrosine nitration (PTN) is a posttranslational modification resulting from oxidative/nitrosative stress that has been implicated in a wide variety of disease states. Characterization of PTN is challenging due to several factors including its low abundance in a given proteome, preferential site modification, multiple target site proximity within unique peptide sequences, and analytical method and instrument limitations. Current analytical techniques are insufficiently sensitive to identify endogenous nitration sites without incorporation of either nitrotyrosine or target protein enrichment. However, enrichment proficiency can also be inadequate. Chemical derivatization of the nitro- moiety can be incomplete or result in undesirable byproduct formation, while …

Love Is In The Air: Olfaction-Based Mate-Odour Identification By Jumping Spiders From The Genus Cyrba, Ana M. Cerveira, Robert R. Jackson

All Peer-Reviewed Publications

Jumping spiders (Salticidae) are known for having good eyesight, but the extent to which they rely on olfaction is poorly understood. Here we demonstrate for the first time that olfactory pheromones are used by two species from the salticid genus Cyrba (C. algerina and C. ocellata). Using a Y-shape olfactometer, we investigated the ability of adult males and females of both species to discriminate between mate and non-mate odour. A hidden spider or a spider's draglines (no spider present) were used as odour sources. There was no evident response by females of either Cyrba species to any tested odour. Males …

The Inflammatory Response Initiated By The Spleen To Ischemic Stroke, Hilary Seifert

USF Tampa Graduate Theses and Dissertations

The peripheral immune system plays a role in delayed neural injury after stroke. This response originates from the spleen as splenectomy prior to middle cerebral artery occlusion (MCAO) in rats significantly reduces infarct volume in the brain. This research is based on the hypothesis that inhibiting the splenic response will reduce neurodegeneration after stroke. Studies in animals have implicated lymphocytes as the immune cell type that is detrimental following MCAO. Interferon gamma (IFNγ) has been identified as a pro-inflammatory cytokine that is also detrimental following stroke. IFNγ is important because it activates microglia and macrophages in a pro-inflammatory nature that …

Common Host-Derived Chemicals Increase Catches Of Disease-Transmitting Mosquitoes And Can Improve Early Warning Systems For Rift Valley Fever Virus, David P. Tchouassi, Rosemary Sang, Catherine L. Sole, Armanda D.S. Bastos, Peter E.A. Teal, Christian Borgemeister, Baldwyn Torto

All Peer-Reviewed Publications

Rift Valley fever (RVF), a mosquito-borne zoonosis, is a major public health and veterinary problem in sub-Saharan Africa. Surveillance to monitor mosquito populations during the inter-epidemic period (IEP) and viral activity in these vectors is critical to informing public health decisions for early warning and control of the disease. Using a combination of field bioassays, electrophysiological and chemical analyses we demonstrated that skin-derived aldehydes (heptanal, octanal, nonanal, decanal) common to RVF virus (RVFV) hosts including sheep, cow, donkey, goat and human serve as potent attractants for RVFV mosquito vectors. Furthermore, a blend formulated from the four aldehydes and combined with …

Harmful Algal Blooms Of The West Florida Shelf And Campeche Bank: Visualization And Quantification Using Remote Sensing Methods, Inia Mariel Soto Ramos

USF Tampa Graduate Theses and Dissertations

Harmful Algal Blooms (HABs) in the Gulf of Mexico (GOM) are natural phenomena that can have negative impacts on marine ecosystems on which human health and the economy of some Gulf States depends. Many of the HABs in the GOM are dominated by the toxic dinoflagellate Karenia brevis. Non-toxic phytoplankton taxa such as Scrippsiella sp. also form intense blooms off the Mexican coast that result in massive fish mortality and economic losses, particularly as they may lead to anoxia.

The main objectives of this dissertation were to (1) evaluate and improve the techniques developed for detection of Karenia spp. blooms …

Optimization In Non-Parametric Survival Analysis And Climate Change Modeling, Iuliana Teodorescu

USF Tampa Graduate Theses and Dissertations

Many of the open problems of current interest in probability and statistics involve complicated data

sets that do not satisfy the strong assumptions of being independent and identically distributed. Often,

the samples are known only empirically, and making assumptions about underlying parametric

distributions is not warranted by the insufficient information available. Under such circumstances,

the usual Fisher or parametric Bayes approaches cannot be used to model the data or make predictions.

However, this situation is quite often encountered in some of the main challenges facing statistical,

data-driven studies of climate change, clinical studies, or financial markets, to name a few. …

Anti-Parasitic And Anti-Bacterial Agents: Studies On 1,4-Dihydropyridines And 2,4-Diaminoquinazolines, Kurt Steven Van Horn

USF Tampa Graduate Theses and Dissertations

Thirty-three 1,4-dihydropyridine diastereomeric pairs were synthesized and the structure-activity relationship studied in a Plasmodium falciparum in vitro model. Twenty-nine of these derivatives contained a 6-position oxygen, with 2.31, 2.32, 2.52 and 2.53 having single and double digit nanomolar activities. This SAR study revealed some insightful information about the 1,4-dihydropyridine substitution pattern. Substitution at the 7-position other than 3,4-dimethoxy severely reduced the activity. 4-phenyl substitution with 2- or 4- halo or methyl formed active compounds while substitution at the 3-position or with methoxy or conjugated aryl systems resulted in inactive compounds. The 2-position was found to majorly affect the activity, with …