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Reaction Enthalpy And Volume Profiles For Excited State Reactions Involving Electron Transfer And Proton-Coupled Electron Transfer, William Antonio Maza

USF Tampa Graduate Theses and Dissertations

Electron transfer, ET, and proton-coupled electron transfer, PCET, reactions are central to biological reactions involving catalysis, energy conversion and energy storage. The movement of electrons and protons in either a sequential or concerted manner are coupled in a series of elementary reaction steps in respiration and photosynthesis to harvest and convert energy consumed in foodstuffs or by absorption of light into high energy chemi-cal bonds in the form of ATP. These electron transfer processes may be modulated by conformational dynamics within the protein matrix or at the protein-protein interface, the energetics of which are still not well understood. Photoacoustic calorimetry …

Eschatology In A Secular Age: An Examination Of The Use Of Eschatology In The Philosophies Of Heidegger, Berdyaev And Blumenberg, John R. Lup, Jr.

USF Tampa Graduate Theses and Dissertations

The topic of eschatology is generally confined to the field of theology. However, the subject has influenced many other fields, such as politics and history. This dissertation examines the question why eschatology remained a topic of discussion within twentieth century philosophy. Concepts associated with eschatology, such as the end of time and the hope of a utopian age to come, remained largely background assumptions among intellectuals in the modern age. Martin Heidegger, Nicolai Berdyaev, and Hans Blumenberg, however, explicitly addressed the subject in their philosophies. The impetus of this study is Heidegger's statement, "Being itself is inherently eschatological," which indicates …

A Child Distracted: Understanding The Relationship Between Pediatric Obsessive-Compulsive Disorder And Subjective Well-Being, Joshua M. Nadeau

USF Tampa Graduate Theses and Dissertations

Obsessive Compulsive Disorder (OCD) is a commonly diagnosed mental health condition among children and adolescents, with studies suggesting that OCD has the potential for significant disruption of academic and social performance. Subjective well-being (SWB) represents a non-traditional conceptualization of mental health within the dual factor model, wherein SWB and measures of psychopathology (e.g., problematic levels of internalizing and externalizing behaviors) provide a more comprehensive picture of mental wellness. The current study examined the nature of the relationship between clinical characteristics of pediatric OCD and SWB within school-age youth (N=65) seeking treatment from an outpatient pediatric neuropsychiatric clinic. Additionally, the potential …

Excellent Leadership In Public Relations: A Question Of Experience And The Pr Practitioner, Roelof Oldenbanning

USF Tampa Graduate Theses and Dissertations

To better understand excellent leadership in public relations (PR) of developing and experienced PR practitioners, this study focuses on the important qualities and dimensions of excellent leadership practitioners. This study continues on the work of Meng, Berger, Gower, and Heyman (2012) in their attempt to advance understanding of leadership in the PR field.

According to the study's participants (N = 72): strategic decision-making capability, problem-solving ability, and communication knowledge and expertise are the three most important qualities of excellent leadership. Moreover, respondents rated that communication skills training, individual initiative and desire, and on-the-job experiences lead to excellent PR leadership. About …

Framing Colombian Women's Beliefs, Values And Attitude Towards Sex And Sexual High-Risk Behaviors, Rosa Ore

USF Tampa Graduate Theses and Dissertations

Hispanic immigrants constitute the largest and fastest growing groups of minorities in the United States. According to the 2010 Census, there are 50.5 million Hispanics in the United States, making up 16.3% of the total population (Passel, Cohn & Lopez, 2011). Furthermore, the state of Florida is home to 4,223,806 Hispanics (U.S. Census Bureau, 2010). Because the Hispanic population continues to grow, it is important to study their sexual health behaviors because diseases linked to risky sexual behaviors account for approximately 20,000 U.S. deaths each year, and are linked to a number of adverse reproductive outcomes (Abraido-Lanza, Chao, & Florez, …

Ecological Interactions Influencing Avicennia Germinans Propagule Dispersal And Seedling Establishment At Mangrove-Saltmarsh Boundaries, Jennifer Mcclain Peterson

USF Tampa Graduate Theses and Dissertations

Mangroves and saltmarshes are ecologically important coastal ecosystems; unfortunately, these low-lying coastal ecosystems are vulnerable to global climate change. As sea-levels rise, mangroves are expected to shift their distribution landward towards higher elevation sites that are occupied by other plants, including saltmarsh taxa. Therefore, mangrove recruits at the leading edge of expansion may interact with diverse assemblages of saltmarsh plants, and these interactions could influence the success of mangrove encroachment into higher tidal-elevation areas. The purpose of the research presented here was to investigate empirically the ecological interactions that may influence the recruitment of the black mangrove, Avicennia germinans, …

Analytical Modeling, Perturbation Analysis And Experimental Characterization Of Guided Surface Acoustic Wave Sensors, Onursal Onen

USF Tampa Graduate Theses and Dissertations

In this dissertation, guided surface acoustic wave sensors were investigated theoretically and experimentally in detail for immunosensing applications. Shear horizontal polarized guided surface acoustic wave propagation for mass loading sensing applications was modeled using analytical modeling and characterized by perturbation analysis. The model verification was performed experimentally and a surface acoustic wave immunosensor case study was presented. The results of the immunosensing were also investigated using the perturbation analysis.

Guided surface acoustic wave propagation problem was investigated in detail for gravimetric (or mass loading) guided wave sensors, more specifically for immunosensors. The analytical model was developed for multilayer systems taking …

Biochemical Aspects Of The Thermal Sensitivity And Energy Balance Of Polar, Tropical And Subtropical Teleosts, Eloy Martinez

USF Tampa Graduate Theses and Dissertations

The maintenance of a functional energy balance in ectothermic fauna could be challenging in a thermally disparate environment. Biochemical adaptations at the enzyme and membrane levels allows for a set compensatory mechanism that allow the individual to maintain an energetic surplus, thus allocating energy for growth and reproduction. The present work describes how the energetic machinery in the cell, particularly the mitochondrion, could be affected by temperature changes. More specifically, this work aimed to determine how environmental temperature affects the mitochondria energetic performance of fishes from disparate thermal regimes.

Mitochondrial ATP production efficiency was evaluated in fishes from polar, tropical …

Investigation Of Hsf1 Interacting Partners Via A Genome-Wide Yeast Two-Hybrid Screen, Jamie Elizabeth Mendez

USF Tampa Graduate Theses and Dissertations

Heat shock factor 1 (HSF1) is the master transcriptional regulator of the heat shock response (HSR), an evolutionarily conserved cellular stress response. HSF1 promotes the expression of a variety of molecular chaperones that aid in restoring protein homeostasis upon exposure to proteoxic stress. However, all of the proteins responsible for regulating the HSR together with HSF1 are unknown. A genome-wide yeast two hybrid screen was performed to identify new S. cerevisiae Hsf1 protein interacting partners. Two GAL4 DNA binding domain-Hsf1 fusion proteins (baits) were constructed with mutations in the Hsf1 C-terminal activation domain to dampen Hsf1 mediated auto-activation of the …

Gradients In Season, Latitude, And Sea Ice: Their Effect On Metabolism And Stable Isotopic Composition Of Antarctic Micronekton, Erica H. Ombres

USF Tampa Graduate Theses and Dissertations

Respiration, metabolic enzyme assays, and body composition parameters were measured in the Antarctic krill Euphausia superba during the summer, fall and winter on the Western Antarctic Peninsula (WAP). E. superba of all sizes decrease their metabolism from the summer to the winter. These same parameters were also measured along the WAP during the austral fall 2010. E. superba's enzyme activity indicated that there was a latitudinal gradient to the decline in metabolism along the WAP with the more northerly sites having significantly higher metabolic enzyme activities than the sites to the south.

Carbon (δ¹³C) and nitrogen (δ^{15 …}

Functional And Mutational Analysis Of Human Recq-Like Dna Helicases In Saccharomyces Cerevisiae, Hamed Mirzaei-Souderjani

USF Tampa Graduate Theses and Dissertations

RecQ-like proteins are a family of DNA helicases that are evolutionary conserved from prokaryotes to eukaryotes. A large amount of experimental evidence suggests these proteins have a major role in the maintenance of genome stability. In humans five RecQ like helicase have been identified (RecQL1, BLM, WRN, RecQL4, and RecQL5), three of which are associated with rare genetic disorders with sever chromosomal and developmental abnormalities, and an elevated predisposition to cancer. Among the disease associated RecQ-like helicases, BLM and WRN have been subject to extensive research, while our collective knowledge about the function of RecQL4 is still very limited. Similarly, …

Light-Environment Controls And Basal Resource Use Of Planktonic And Benthic Primary Production, Kara Radabaugh

USF Tampa Graduate Theses and Dissertations

Consumers in marine and estuarine environments have a strong reliance on planktonic and benthic primary production. These two basal resources form the foundation of aquatic food webs, yet the abundance of phytoplankton and benthic algae are frequently inversely related due to competition for light and nutrients. As a result, optimal habitats for benthic and planktonic consumers vary spatially and temporally. To investigate these trends, three studies were conducted focusing on light attenuation and basal resources in a bay, river, and on a continental shelf.

δ¹³C and δ¹⁵N stable isotopes can be used as endogenous tracers to …

Do Mathematics And Reading Competencies Integrated Into Career And Technical Education Courses Improve High School Student State Assessment Scores, Kristin Bowles Pierce

USF Tampa Graduate Theses and Dissertations

A quasi experimental study tested a contextual teaching and learning (CTL) model for integrating reading and mathematics competencies through 13 introductory Career and Technical Education (CTE) courses. Volunteer CTE Lead Teachers with assistance from academic teachers, developed integrated units. The purpose of this study was to determine whether students who participated in CTE courses that integrated core mathematics and reading standards performed better on a test of mathematics and reading skills compared to students who participated in traditional, non-integrated courses. The treatment group consisted of students in the 13 introductory courses taught by the CTE Lead Teachers and the control …

The Baby Blues: Mothers' Experiences After Adoption, Brigette Barno Schupay

USF Tampa Graduate Theses and Dissertations

ABSTRACT

It is not uncommon for new mothers to experience depression. However, depression after the arrival of a child is not limited to biological mothers. The term Post-Adoption Depression Syndrome (PADS) was created to capture the unique type of depression that may occur after adopting a child. The nature and prevalence of depression after adoption is still largely unknown since there is little published research exploring the experiences of mothers in the first year after adopting a child. The characteristics of this disorder are reportedly comparable to postpartum depression in a biological mother. A review of postpartum literature reveals risk …

High School Students In College-Level Classes: Associations Between Engagement, Achievement, And Mental Health, Rachel Anne Roth

USF Tampa Graduate Theses and Dissertations

Student engagement is a multifaceted construct gaining increased interest within the fields of psychology and education. Current literature suggests that student engagement is linked to important student outcomes including academic achievement, psychopathology, and mental wellness; however, there is a dearth of studies that have examined all components of student engagement simultaneously as they relate to the aforementioned outcomes. Additionally, past literature has found support for a decreasing trend in student engagement across the school years, but less attention has been paid to student engagement in the high school years. Among high school students, a particular subgroup has been virtually ignored: …

Sirt1 Regulation Of The Heat Shock Response In An Hsf1-Dependent Manner And The Impact Of Caloric Restriction, Rachel Rene Raynes

USF Tampa Graduate Theses and Dissertations

The heat shock response (HSR) is the cell's molecular reaction to protein damaging stress and is critical in the management of denatured proteins. Activation of HSF1, the master transcriptional regulator of the HSR, results in the induction of molecular chaperones called heat shock proteins (HSPs). Transcription of hsp genes is promoted by the hyperphosphorylation of HSF1, while the attenuation of the HSR is regulated by a dual mechanism involving negative feedback inhibition from HSPs and acetylation at a critical lysine residue within the DNA binding domain of HSF1, which results in a loss of affinity for DNA. SIRT1 is a …

Utilizing Habit Reversal And Contingency Contracting To Impact Eating Habits With Obese Individuals, Christopher Thomas Scull

USF Tampa Graduate Theses and Dissertations

One of the most difficult set of behaviors for individuals to engage in is weight management. The United States boasts an astonishing 35% obesity rate in adults while children show almost a 20% obesity rate. These statistics identify the importance of developing effective methods to help change an individual's eating habits. There are many eating habits that may contribute to an individual gaining or losing weight over a period of time. Simplified habit reversal (SHR) has an impressive history in shaping desirable responses to compete with many different types of habitual behaviors. The present study extended the SHR procedure to …

Direct And Indirect Effects Of Agrochemicals On Bacterial Pathogens And Fecal Indicator Bacteria, Zachery Staley

USF Tampa Graduate Theses and Dissertations

The presence of agrochemical residues in both urban and agricultural water bodies has become ubiquitous, often producing deleterious effects in the impacted watershed including reductions in biodiversity, alterations in species interactions, and toxicity to non-target organisms. While these effects have been studied on metazoan consumers, the consequences of agrochemical contamination on microorganisms, such as bacteria, protozoa, and viruses, are poorly understood. Agrochemicals could act directly on microorganisms, including pathogens, by either facilitating their survival or decreasing their abundance. Further, a multitude of indirect effects of agrochemicals on microorganisms are possible, whereby agrochemicals alter predation, competition, or parasitism on or available …

Synthesis And Properties Of Polymer Nanocomposites With Tunable Electromagnetic Response, Kristen Lee Stojak

USF Tampa Graduate Theses and Dissertations

Multifunctional polymer nanocomposites (PNCs) are attractive for the design of tunable RF and microwave components such as flexible electronics, attenuators, and antennas due to cost-effectiveness and durability of polymeric matrices. In this work, three separate PNCs were synthesized. Magnetite (Fe3O4) and cobalt ferrite (CFO) nanoparticles, synthesized by thermal decomposition, were used as PNC fillers. Polymers used in this work were a commercial polymer provided by the Rogers Corporation (RP) and polyvinylidene fluoride (PVDF). PNCs in this thesis consist of Fe3O4 in RP, CFO in RP, and Fe3O4 in PVDF. Characterization techniques for determining morphology of the nanoparticles, and their resulting …

Regulation Of The Tumor Suppresser P53 And Survivin By Ras And Ral Gtpases:Implications For Malignant Transformation, Awet G. Tecleab

USF Tampa Graduate Theses and Dissertations

Abstract

Although the critical role of the small GTPases Ras and Ral in oncogenesis has been well documented, much remains to be investigated about the molecular mechanism by which these GTPases regulate malignant transformation. The work under this thesis made two major contributions to this field. The first is the discovery that K-Ras, RalA and/or RalB are required for the maintenance of the high levels of the anti-apoptotic protein survivin in some human cancer cells, and the second is the demonstration that down regulation of K-Ras, RalA and/or RalB, but not Raf-1 or Akt1/2, stabilizes the tumor suppressor p53 and …

Archaeological Site Distribution In The Apalachicola/Lower Chattahoochee River Valley Of Northwest Florida, Southwest Georgia, And Southeast Alabama, Adam M. Schieffer

USF Tampa Graduate Theses and Dissertations

This research examines and compares the distributions of archaeological sites and materials in order to investigate native settlement patterns and resources use throughout 12,000 years of prehistory and protohistoric time within the Apalachicola/Lower Chattahoochee River valley of northwest Florida, southwest Georgia, and southeast Alabama. Geographic Information Systems (GIS) are used to map the distributions of sites from different time periods and to explore their relation to various environmental characteristics that are now available in digital format. I employ tools now available in GIS to examine several longstanding research questions and expand upon archaeological interpretations within this region, where the University …

"When You Tell Them, Your Secret Is Out There": Experiences Of Sexuality And Intimacy Among Hiv Positive Black Women, Mackenzie Rae Tewell

USF Tampa Graduate Theses and Dissertations

HIV/AIDS infections disproportionately impact African Americans within the United States. In 2010, black Americans made up 12 percent of the United States population, yet accounted for 44 percent of new HIV/AIDS infections (Kaiser Family Foundation 2013). The majority of black women (85 percent) are infected with the virus through heterosexual contact, meaning it is critical examine their sexual lives in order to gain insight into this infection within this population (CDC 2011b). Through semi-structured interviews at a Tampa, Florida AIDS service organization, this study presents the experiences of sexuality and intimacy among HIV positive black women. Results demonstrate that HIV …

Computational Approaches For Structure Based Drug Design And Protein Structure-Function Prediction, Sai Lakshmana Kumar Vankayala

USF Tampa Graduate Theses and Dissertations

This dissertation thesis consists of a series of chapters that are interwoven by solving interesting biological problems, employing various computational methodologies. These techniques provide meaningful physical insights to promote the scientific fields of interest. Focus of chapter 1 concerns, the importance of computational tools like docking studies in advancing structure based drug design processes. This chapter also addresses the prime concerns like scoring functions, sampling algorithms and flexible docking studies that hamper the docking successes. Information about the different kinds of flexible dockings in terms of accuracy, time limitations and success studies are presented. Later the importance of Induced fit …

Investigations For Utilizing Pteropods As Bioindicators Of Environmental Change Along The Western Antarctic Peninsula, Paul Mark Suprenand

USF Tampa Graduate Theses and Dissertations

Pteropods are holoplanktonic gastropod molluscs found globally. Although species diversity is greater at lower latitudes, species abundance is greater at temperate and polar latitudes. Declines in pteropod populations have not only been correlated to declines of their major predators, but pteropods have also been used as bioindicators of global environmental changes such as ocean acidification. With high latitude abundances, pteropods provide significant sustenance for species such as the Atlantic salmon in the Atlantic Ocean and Pleuragramma antarcticum in the Southern Ocean. Because pteropods eat phytoplankton and other pteropods, factors that affect pteropod abundance influence many trophic levels. This dissertation explores …

Asymmetric Nitrene Transfer Reactions With Azides Via Co(Ii)-Based Metalloradical Catalysis (Mrc), Jingran Tao

USF Tampa Graduate Theses and Dissertations

Asymmetric nitrene transfer reactions via metalloradical catalysis (MRC) with

azides has attracted research interest because of its fundamental and practical importance.

The resulting nitrogen-containing units are recurrent motifs in biologically important

molecules and can serve as versatile precursors in organic synthesis.

The [Co(D2-Por*)] have emerged as a new class of catalysts for asymmetric

aziridination and C-H amination. These metalloradical catalysts have been shown to be

highly effective for the asymmetric intermolecular aziridination of a broad scope of

substrates with different classes of azides with excellent to good enantioselectivity. The

intramolecular C-H amination utilizing various azides can allow for the construction …

Eco-Epidemiology Of Eastern Equine Encephalitis Virus, Patrick Vander Kelen

USF Tampa Graduate Theses and Dissertations

ABSTRACT

Eastern Equine Encephalitis virus (EEEV) is an alphavirus with high pathogenicity in both humans and horses. Florida continues to have the highest occurrence of human cases in the USA, with four fatalities recorded in 2010. Unlike other states, Florida supports year-round EEEV transmission. This research uses Geographic Information Science (GIS) to examine spatial patterns of documented sentinel seroconversions and horse cases in order to understand the relationships between habitat and transmission intensity of EEEV in Florida. Sentinel sites were categorized as enzootic, periodically enzootic, and negative based on the amount of chicken seroconversions to EEEV. Sentinel sites were analyzed …

Phenotypic And Genotypic Analysis Of In Vitro Selected Miltefosine Resistant Leishmania Donovani, Brian A. Vesely

USF Tampa Graduate Theses and Dissertations

Abstract

Visceral leishmaniasis is a devastating neglected parasitic disease caused by infection with Leishmania donovani. It life cycle has two stages with promastigote (insect stage) and amastigote (animal stage) morphologies. Miltefosine is currently the only commercially available oral drug available to treat leishmaniasis and recent evidence suggests clinical resistance has emerged. Due to the importance of this drug and the scarcity of new drugs in the pipeline, work has been done on understanding the mechanism(s) of miltefosine, yet the mechanism of action for resistance is still not known.

In previous studies investigators generated miltefosine resistance on the insect vector stage …

Reaction Formation And Homophobia: An Erp Examination, Krista Grace Yakub

USF Tampa Graduate Theses and Dissertations

Homophobia in men may be, in part, due to reaction formation rooted in unacceptable same-sex attraction. Previous studies have not confirmed a covert same-sex attraction in homophobic men, which is necessary for a reaction formation theory of homophobia. This study sought to reveal possible covert same-sex attraction in homophobic men. In this study, heterosexual and homosexual male erotic images were presented in a passive S1/S2 stimulus prediction design to 48 self-identified heterosexual participants, grouped by homophobia. Three event-related potential responses related to valenced emotional processing were examined: the medial frontal negativity (MFN), the late positive potential (LPP), and the positive …

The Sopranos Experience, Eli Benjamin Weidinger

USF Tampa Graduate Theses and Dissertations

My thesis explores what I call the "Sopranos Experience," which draws upon both the historical conventions of the gangster genre as well as the aesthetics and economics of pay-cable television to complicate The Sopranos' (HBO, 1999-2007) psychological relationship with the 21st-century, neoliberal American audience. The Sopranos Experience explicates how wavering identifications and dis-identifications that develop for the spectator through the series' form and content draw the responsibility of an audience away from moral ultimatums that attempt to finalize their experience with the genre, and towards a more personal ethical entanglement with the characters and their socioeconomic anxieties and desires. The …

Predicting Academic Outcomes For Third Grade Students: Examining The Reading Achievement Of Diverse Students Using The Diagnostic Lens Of The Dynamic Indicators Of Basic Early Literacy Skills, Zhivago Trevino Adderley

USF Tampa Graduate Theses and Dissertations

Despite the billions of dollars spent in the last forty years, America's efforts toward closing the achievement gaps among diverse learners and their receptive counterparts have not been realized. Limitations noted in previous research discussed the need to examine the unique contributions of diverse learner variables as a way of determining their specific academic needs. The purpose of this study was to examine the intra- and inter-group growth trajectories of two diverse student groups (English Language Learners and Students with Specific Learning Disabilities) on reading achievement. The study employed a longitudinal, quasi-experimental research design utilizing archival data from 26,947 students' …