Digital Commons Network^™

Fabrication Of Responsive Polymer Brushes For Patterned Cell Growth And Detachment, Ashley B. Sutherland

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Patterned polymer brushes are used in biological applications to enhance cell function and to achieve selective cell growth. These patterned surfaces have a variety of applications, including cell sheet harvesting and tissue engineering. This work describes creation of chemical, topological, and responsive patterns on glass by sequential surface-initiated polymerization of poly(N-isopropylacrylamide)-co-(2-hydroxyethyl methacrylate) (pNIPAM-co-HEMA) and subsequent use of UV lithography to pattern the surface with pNIPAM. The cell adhesion peptide, RGD, is then immobilized on the surface of the copolymer pHEMA via DSC-coupling to spatially enhance cell adhesion properties. By culturing cells at 37 degrees celsius, cells selectively grow on DSC-activated …

Effects Of Ang 1-7 And Endothelial Microvesicles On Ang Ii-Induced Dysfunction And Apoptosis In Cerebral Endothelial Cells, Xiang Xiao

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Angiotensin II (Ang II) induces endothelial dysfunction and is implicated in the pathogenesis of vascular diseases. Angiotensin 1-7 (Ang 1-7) has been reported to counteract many deleterious effects of Ang II. Endothelial microvesicles (EMVs) are small membrane vesicles released from endothelial cells (ECs) undergoing stress and apoptosis. But their functions are largely unknown. In this study, we investigated the effects of Ang 1-7 and EMVs on apoptosis and dysfunction of human brain microvascular endothelial cells (HbmECs). Reactive oxygen species (ROS) and nitric oxide (NO) production, and Nox2, p-Akt/Akt, p-eNOS/eNOS expression were analyzed. We found that Ang II dose-dependently induced HbmEC …

Likely Successors Of Ash Species In Response To The Emerald Ash Borer In Ohio Forests, Brian Michael Good

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Invasive species have the capability to alter landscapes and change the composition of a forest in a very short time. The recent invasive pest, Agrilus planipennis, emerald ash borer, was unintentionally introduced to the United States via ship route to Michigan. The pest attacks and kills all five native ash species in Ohio. This study focused on an area in west central Ohio not yet affected by the borer. Ash centered plots were used to record all species and sizes (diameter at breast height) within a 5m radius of a central ash tree. Plots ranged in topography and all five …

Novel Approaches For The Eradication Of Hiv Latently Infected Cells, Sally Al Ali

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The development of a suitable experimental cell model to study HIV latency in primary cells could have a massive effect on the current approaches to eradicate virus in latently infected cells. The main proposal of this paper is to develop an in vitro HIV cell model that represents HIV latency in vivo, then to create a more effective viral vector in order to target HIV reservoirs. For this goal, a directed evolution method is suggested to be used in order to mutate the AAV cap gene to generate a recombinant AAV vector that is capable of infecting primary resting CD4+ …

Examination Of The African-American Father-Daughter Relationship: Application Of The Marschack Interaction Method, Amber N. Thornton

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Early research on the Black family implied an enormous lack of African-American father presence and involvement among African-American fathers. However, more current research negates those findings by highlighting the contributions African-American fathers make when parenting their children (Coley, 2003; Cooper, 2009; Mandara, Murray & Joyner, 2005). In spite of facing unique psychological and social challenges, such as disproportionate levels of poverty, and race related social barriers, such as the invisibility syndrome (Franklin & Boyd-Franklin, 2000), researchers who have studied the African-American father-daughter dyad have discovered that this relationship has many benefits for African-American girls, including cognitive and academic enhancements, reductions …

Barriers To Group Therapy For Latino College Students In The United States, Michelle C. Stoyell

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While group therapy appears to be gaining popularity at University Counseling Centers, scant research has been done on Latino students' group therapy participation. The present study examined the barriers that Latino college students face in considering whether to join group therapy. Participants (N=81) completed three measures. The first measure was a demographic questionnaire. The second measure was a modified version of the Barriers Scale, originally developed by Harris (2012), a 44-item survey in which participants were assessed in terms of: their willingness to consider group therapy, group therapy modality preferences, expectations of group therapy, expectations of group members, expectations of …

Mindfulness-Based Interventions And Processes Of Change: A Conceptual Model For Clinicians, Shalagh A. Frantz

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Mindfulness-based interventions are receiving a great deal of attention from clinicians and researchers and research in this field is increasing dramatically in the past decade. Much of this research is consistent with positive psychotherapeutic outcomes for a wide range of presenting clinical issues. As with any seemingly successful psychotherapeutic treatment, it can be helpful to understand the processes of change underlying mindfulness-based interventions that are responsible for observed positive outcomes. In doing so, the effective components of an intervention can be refined and perfected, while components deemed unneeded can be appropriately discarded. This dissertation critically reviews the literature's current understanding …

Co2/Ph Signaling Of Respiratory Control Neurons In The Bullfrog, Lithobates Catesbeianus: Development Of A Comparative Model, Joseph M. Santin

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The locus coeruleus (LC) in the brainstem senses alterations in CO₂/pH and influences ventilatory adjustments that restore blood gas values to starting levels in bullfrogs (Lithobates catesbeianus). We hypothesized that neurons of the bullfrog LC are sensitive to changes in CO₂/pH and that chemosensitive responses are intrinsic to individual neurons. In addition, we hypothesized putative respiratory control neurons of the bullfrog LC would be stimulated by hypercapnic acidosis within physiological ranges of PCO₂/pH. 84% of LC neurons depolarized and increased firing rates during exposure to hypercapnic acidosis (HA). A pH dose response …

Natural Language Document And Event Association Using Stochastic Petri Net Modeling, Michael Thomas Mills

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The purpose of this research is to design and implement a new methodology that captures the natural language understanding of events from English natural language text and model it using Stochastic Petri Nets. To establish a baseline of recent natural language processing (NLP) and understanding (NLU) research, two surveys are presented. One is a general survey in NLP and NLU methodologies for processing multi-documents. It summarizes and presents methodologies in terms of their features, capabilities, and maturity. The second survey focuses on graph-based methods for NL text processing and understanding and analyzes them in terms of their functional descriptions, capabilities …

Computational Modeling Of Nanosensors Based On Graphene Nanoribbons Including Electron-Phonon Effects, Kirti Kant K. Paulla

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We investigate detection mechanisms of real time sensors, based on ultra-thin (single and bi-atomic layer thick) and ultra-narrow (~1nm) graphene nanoribbons (GNRs), using first principle based theoretical methods. In the first part of this study we study the electronic and magnetic structures of bilayer graphene nanoribbons (BGNRs) beyond the conventional AA and AB stackings, by using density functional theory within both local density and generalized gradient approximations (LDA and GGA). Our results show that, irrespective of the method chosen, stacking arrangements other than the conventional ones are most stable, and result in significant modification of BGNRs characeristics. The most stable …

Wing Damage Effect On Dragonfly's Aerodynamic Performance During Takeoff, Kuo Gai

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Insect wing damage resulted from living environment or predation commonly happens in nature. This usually results in deterioration of insect's flight performance and as a consequence, the insect needs adjustment of flapping wings to compensate the effect from the wing loss. In this study, a dragonfly (Erythemis simpliciolis) with and without wing loss is chosen to study the change of aerodynamic performance of flapping wings. Three cases including flight with intact wings (IW), flight with one-sided forewing damage (OFD), and flight with double-side forewing damage (DFD) are determined. An integrated study using high-speed photogrammetry, three-dimensional surface reconstruction, and direct numerical …

Overview Of The Large-Scale Biosphere–Atmosphere Experiment In Amazonia Data Model Intercomparison Project (Lba-Dmip), Luis Gustavo Goncalves De Goncalves, Jordan Borak, Marcos Heil Costa, Scott R. Saleska, Ian Baker, Natalia Resprepo-Coupe, Michel Nobre Muza, Benjamin Poulter, Hans Verbeeck, Joshua B. Fisher, Altaf Arain, Phillip Arkin, Bruno P. Cestaro, Bradley Christoffersen, David Galbraith, Xiaodan Guan, Bart Van Den Hurk, Kazuhito Ichii, Hewlley Acioli Imbuzeiro, Atul Jain, Naomi Levine, Chaoqun Lu, Gonzalo Migues-Macho, Debora Roberti, Alok Sahoo, Koichi Sakaguchi, Kevin Shaefer, Mingjie Shi, James Shuttleworth, Hanqin Tian, Zong-Lian Yang, Xubin Zeng

United States National Aeronautics and Space Administration: Publications

A fundamental question connecting terrestrial ecology and global climate change is the sensitivity of key terrestrial biomes to climatic variability and change. The Amazon region is such a key biome: it contains unparalleled biological diversity, a globally significant store of organic carbon, and it is a potent engine driving global cycles of water and energy. The importance of understanding how land surface dynamics of the Amazon region respond to climatic variability and change is widely appreciated, but despite significant recent advances, large gaps in our understanding remain. Understanding of energy and carbon exchange between terrestrial ecosystems and the atmosphere can …

Reading (Into) Windsor: Presidential Leadership, Marriage Equality, And Immigration Policy, Victor C. Romero

Faculty Scholarship

Following the demise of the federal Defense of Marriage Act in United States v. Windsor, the Obama Administration directed a bold, equality-based reading of Windsor to immigration law, treating bi-national same-sex couples the same as opposite-sex couples. This Essay argues that the President's interpretation is both constitutionally and politically sound: Constitutionally, because it comports with the Executive's power to enforce immigration law and to guarantee equal protection under the law; and politically, because it reflects the current, increasingly tolerant view of marriage equality. Though still in its infancy, President Obama's policy of treating same-sex beneficiary petitions generally the same as …

Sexual Orientation Of Fatherhood, Dara Purvis

Faculty Scholarship

In this Article, I examine how same-sex fathers affect the perception of heterosexual caretaking fathers - and by extension, could affect the perception of heterosexual non-caretaking mothers. I conclude that gay stay-at-home fathers offer a provocative opportunity to broaden societal views of men and caregiving more generally, and argue that greater recognition of parents who counteract gender stereotypes - even where the recognition might arguably lessen women's rights in family law - ultimately helps women as well as children and nontraditional parents. Part I discusses fathers, particularly stay-at-home fathers, the practical problems fathers face combining work and caregiving responsibilities, and …

Pass Parallel Privacy Standards Or Privacy Perishes, Anne T. Mckenna

Faculty Scholarship

No abstract provided.

Success And Backlash: The Remarkable (Continuing) Story Of The Clean Water Act, William L. Andreen

Articles

The Clean Water Act has produced a tremendous amount of progress over the past forty years Both municipal and industrial discharges have declined sharply the loss of wetlands has been cut decisively and water quality has broadly improved Much more work however remains to be done Not surprisingly given the limits of human forethought and political capacity the original statutory design was not perfect Furthermore the statutes implementation has not been perfect due in large part to often straitened budgets instances of bureaucratic timidity and lethargy and the hostility that some administrations and some Congresses have exhibited towards strong environmental …

The Effect Of Input Modality On Pronunciation Accuracy Of English Language Learners, Marcella A. Farina Phd

Electronic Theses and Dissertations

The issues relative to foreign accent continue to puzzle second language researchers, educators, and learners today. Although once thought to be at the root, maturational constraints have fallen short of definitively accounting for the myriad levels and rates of phonological attainment (Bialystok & Miller, 1999, p. 128). This study, a Posttest-only Control Group Design, examined how the pronunciation accuracy of adult, English language learners, as demonstrated by utterance length, was related to two input stimuli: auditory-only input and auditoryorthographic input. Utterance length and input modality were further examined with the added variables of native language, specifically Arabic and Spanish, and …

Effect Of Rat Strain Stereotactic Coordinates On Infarct Volume, Saagar K. Sanghvi

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Ischemic stroke makes up 87% of all hospital-admitted stroke cases annually; the primary treatment for these cases is intravenous administration of tPA within a 3.5 hour window from stroke onset. A long-term delayed ischemic stroke treatment proposed by this study was a combination of the pharmaceuticals Fluoxetine (SSRI), Simvastatin (statin), and ascorbic acid (Vitamin C). 51 adult rat subjects (10-12 months of age; 44 Sprague Dawley, 7 Long Evans) were given a combination of the drugs for 31 days. Drugs were given through voluntary oral administration via sugar cookie-dough balls to reduce inhibition of neurogenesis through stress-related glucocorticoid production. Drug …

Selective Free-Standing Through-Wafer Porous Silicon Membrane (Sftpsm) For Integrated Meta-Material Devices, Bella Liu Yao

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Meta-materials have attracted considerable attention due to their unique electromagnetic properties, such as negative permittivity (ε), negative magnetic permeability (μ), and negative index of refraction. These devices have shown broad flexibility in compact phase shifter, backward leaky-wave antenna, and high-directivity coupled-line coupler applications; however, integrated devices utilizing meta-material structures have not yet surfaced due to a lack of integration technology.

In this work, a novel concept of integrated meta-materials is presented, using free-standing porous silicon (PS) membranes of periodic magnetic wire arrays (PMWA). The free-standing PS membranes were fabricated on p-type silicon substrates through electrochemical anodization. Systematic investigations have been …

Analysis Of Human Echolocation Waveform For Radar Target Recognition, Kandarp Patel

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Some blind humans have developed the remarkable capability of echolocation, similar to the type used by mammals such as the bat, dolphin and whale. This population of human has shown the ability to classify targets based on their location, size, shape and material in diverse environmental conditions simply by listening to the reflected echoes of tongue clicks generated by their mouth. To date, much of the research into human echolocation has been confined exclusively to behavioral science and the analysis is inconsistent with the approaches used in engineering. The waveforms used in current radar systems appear different to those typical …

Enhancing Herbicide Efficacy On Reed Canary Grass (Phalaris Arundinacea) By Testing A Plant Growth Hormone, Application Times, And Herbicide Type, Denise Lynn Fong

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Phalaris arundinacea, also known as reed canary grass (RCG), is a non-native invasive grass that thrives in floodplains. RCG plants displace native flora which reduces diversity in otherwise species rich wetlands. This is a widespread problem throughout many parts of the USA. RCG can grow by its seeds or its rhizomes. Its seeds are capable of surviving long periods in soil. RCG can potentially be controlled by multi-year treatments in early spring and early fall with broad spectrum or grass specific herbicides. The goal of this study was to optimize methods to control RCG, in order to increase species …

Hpai H5n1: A Global Pandemic Concern, With Implications For Pandemic Preperation And Public Health Policy, Lauren M. Koontz

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Research on highly pathogenic avian influenza A (HPAI) H5N1 has gained much attention in recent years due to its devastating impact within the bird population. While transmission to humans is rare, infections are fatal in more than half of the cases. One of the main concerns among the scientific community is the ability for this virus to mutate in a way much like seasonal influeanza that would allow it to be transmitted efficiently among the human population. Two researchers were able to separately create mutant isolates of H5N1 influenza A virus that could be transmitted via the respiratory route between …

Non-Contact Probes For Characterization Of Thz Devices And Components, Mads Jacob Hedegaard Larsen

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One of the challenges in developing semiconductor devices and integrated circuits for the terahertz (THz) region is their electrical characterization. The most common technology for their characterization is presently metal-to-metal, DC-coupled contact probes, which operate up to 750 GHz but are expensive and fragile.

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In this Master's Thesis, we investigate through numerical simulation and analysis the electromagnetic properties of a novel, AC-coupled probe. The new probe couples radiation from the device or circuit-under-test (DUT) via polarization current, which is then transformed to conduction current in the probe and down-converted in frequency to baseband by an optically-pumped photomixer. Finite-element simulations …

Quantitative Analysis Of The Polarity Reversal Pattern Of The Earth's Magnetic Field And Self-Reversing Dynamo Models, Patrick Shane Craig

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The geologic record documents that the Earth's magnetic field has reversed 284 times over the past 160 Myr. This study uses two methods to analyze the scaling properties of the pattern of geomagnetic reversals and to analyze the scaling properties of reversals output by two mathematical self-reversing dynamo models, the Rikitake (1958) two-disk dynamo and the magnetohydrodynamic (MHD) model of Driscoll and Olson (2011). The first analysis method plots the duration versus cumulative probability for geomagnetic polarity intervals ranging between 0.01 and 35 Myr. The Kolmogorov-Smirnov statistic is used to determine the optimal minimum value of xmin at which power …

Characterization Of Stimulation-Induced Volume Changes In The Ca1 Region Of Rat Hippocampus Slices, Amanda Brooke Gutwein

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Intrinsic optical signals (IOS) were used to evaluate volume regulation mechanisms during Schaffer collateral stimulation-induced volume changes in hippocampal slices. The effects of stimulation frequency, synaptic function, ionotropic glutamate receptor (iGluR) activation, glutamate uptake, and volume regulatory anion channels (VRAC) were examined with IOS. Data were analyzed using ANOVA with Dunnett's post hoc test (p<0.05 indicated significance). IOS changes were stimulation frequency dependent between 1 Hz and 10 Hz with full recovery of IOS within 5 min. Synaptic blockage reduced the rate of swelling by 81% compared to the control and the IOS did not fully recover. Recovery rate was reduced with iGluR inhibition. Inhibition of glutamate uptake reduced the rate of swelling by 70% compared to vehicle controls. During stimulation, action potentials, iGluR activation, and glutamate uptake contribute to swelling. Regulatory volume mechanisms during stimulation were glutamate-mediated via iGluRs and VRAC independent.

Palaeoecological Analysis Of The Decline In Stromatolite Abundance During The Ordovician Period, Noran Mhm El-Sherif

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A stromatolite is a laminated benthic microbial deposit. Its uniqueness arises from being present since the Precambrian to the present. Stromatolites recorded a peak time during the Mesoproterozoic, subsequently they witnessed abrupt rises and falls in abundance with the steepest decline in the Ordovician period, from which it never recovered from. There is no consensus yet regarding the reasons behind the decline of stromatolites. Thus the decline of these microbial deposits remains an enigma. Additionally, a literature gap exists regarding the reasons that specifically led to the Ordovician decline. Accordingly, the focus of this literature-based MSc. thesis is to find …

Bull's-Eye Structure With A Sub-Wavelength Circular Aperture, Masoud Zarepoor

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This research focuses on improving the spatial resolution of terahertz (THz) imaging. Using a sub-wavelength aperture in a metal ground plane is the most common way to improve the spatial resolution, but a sub-wavelength aperture has low radiation transmission. To increase the radiation transmission of a sub-wavelength aperture, periodic circular grooves were put in the metal around the aperture. The sub-wavelength circular aperture and the periodic circular grooves around it are known as the bull's-eye structure. The bull's-eye structure shows high radiation transmission at its design frequency, and it also has sub-wavelength spatial resolution. This research designed the bull's-eye structure …

Abiotic Degradation Of Chlorinated Hydrocarbons (Chcs) With Zero-Valent Magnesium (Zvm) And Zero-Valent Palladium/Magnesium Bimetallic (Pd/Mg)-Reductant, Fang Yu

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Chlorinated hydrocarbons (CHCs) in groundwater can be treated by monometallic and bimetallic metal reductants through abiotic degradation. The breakdown of CHC is achieved by gaining electrons from those reductants and removing chlorines from CHC molecules to transform the CHCs into less chlorinated compounds. As a proven technology in groundwater treatment, permeable reactive barriers (PRBs) have been used to passively treat contaminated groundwater, in which granular metals can be used as reactive materials. This study explored the abiotic degradation of CHCs by zero-valent magnesium (ZVM) and bimetallic palladium/magnesium (Pd/Mg) reductants. Different CHCs (carbon tetrachloride, chloroform, dichloromethane (DCM), 1,2-dichloroethane (1,2-DCA), 1,1,2-trichloroethane (1,1,2-TCA), …

Long-Term Effects Of Deer Browsing On Northern Wisconsin Forest Plant Communities, Danielle Rae Begley

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The effects of excessive white-tailed deer (Odocoileus virginianus) browsing on forest understory plant communities are well-documented. However, these studies are usually short-term, and fail to focus on phylogenetic diversity and additional variables that may explain species composition. This study examined ecological and phylogenetic diversity, vegetation structure, and light (% insolation) in short (7 year) and long-term (22 year) exclosures (and paired controls) to identify if competitive exclusion occurs in long-term exclosures. A deer browsing susceptibility index (DBS) was also developed to identify species reliant on exclosures for persistence. Statistical analysis using 2-way ANOVAs showed increased percent cover, vegetation height, and …

The Effects Of Multicollinearity In Multilevel Models, Patrick Carl Clark Jr.

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This study examined a method for calculating the impact of multicollinearity on multilevel modeling. The major research questions concerned a) how the simulation design factors affect (multilevel variance inflation factor) MVIF, b) how MVIF affects standard errors of regression coefficients, and c) how MVIF affects significance of regression coefficients. Monte Carlo simulations were conducted to address these questions. Predictor relationships were manipulated in order to simulate multicollinearity. Findings indicate that a) increases in relationships among Level 1 predictors and also relationships among Level 2 predictors led to increased MVIF for those specific variables, b) as MVIF increases for a predictor, …