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Transformations In Body And Cuisine In Rural Yucatan, Mexico, Lauren Wynne

Anthropology and Sociology Faculty Publications

Many twentieth-century ethnographic accounts of the rural Yucatec Maya analyze the accruement of culinary knowledge as a crucial part of the process of socialization, by which children become adults (e.g., Gaskins 2003; Greene 2002). Indeed, in the rural town of Juubche, fairly predictable culinary milestones continue to mark the lives of girls and women. Some young women, however, are using their access to new foods and related knowledge to challenge both local hierarchies of expertise and ideologies of racial difference. For many young women in Juubche, there is a link between consuming new foods and identifying oneself with larger Yucatecan …

Primarily Math: Success Stories

NebraskaMATH Materials

Exceeding expectations: Primarily Math overview, pg. 3

Realizing her full potential: Marni Driessen, pg. 6

Taking on math’s ‘big’ ideas: Kristy Kennedy, pg. 8

Making a greater impact: Alysia Augustus, pg. 10

Working as a team pays off: Cindy Beaman, Dana Henry and Amber

Woitaszewski, pg. 12

Stretching her skills in math: Amy Davidson, pg. 14

Rethinking and reaffirming: Frances Merica, pg. 15

Connecting school to home: Allie Guiney, pg. 16

Leadership roles take shape: Lacey Konwinski, pg. 18

Learning value of listening: Andrew Boone, pg. 19

‘There is no fear of failing’: Jessica Tewalt, pg. 20

Coaches’ impact visible, …

A Troubled Agreement For Troubled Waters: How An Amended Boundry Waters Treaty Can Solve The Great Lakes Agreement's Fatal Flaws, Paul Shugar

Global Business Law Review

Great Lakes water fuels $4.2 trillion of gross-domestic product (“GDP”), making the Great Lakes Region the largest bi-national regional economy in the world. But what are the United States and Canada doing to protect the world’s largest readily available freshwater resource? The Great Lakes-St. Lawrence River Basin Sustainable Water Resources Agreement’s failures show that Canada and the United State must amend the outdated Boundary Waters Treaty of 1909. This amended treaty would provide a uniform approach to regulating the Great Lakes so the states and provinces on both sides of the border must play by the same rules regarding water …

Patent Variation: Discerning Diversity Among Patent Functions, Jessica Silbey

Loyola University Chicago Law Journal

This Article describes and analyzes qualitative interview data collected over a five-year period. The goal of the interviews was to explore the roles of intellectual property (“IP”) in IP rich fields. Interviews were with diverse actors in a wide-range of industries: film, book publishing, visual arts, internet commerce, biology, engineering, chemistry, computer science. The data described and analyzed in this Article focuses on the specific question about the diverse functioning of patents in the subset of interviewees who are scientists and engineers, their lawyers and business partners. The Article proceeds in two parts. Part I describes the empirical dimension of …

Detailed Phosphorus Geochemistry Of Sediments From The Equatorial Proto-Atlantic At Demerara Rise During Oceanic Anoxic Event 2, Mary Elizabeth Smith

All-Inclusive List of Electronic Theses and Dissertations

Oceanic anoxic events (OAE) are associated with increased organic matter burial and possibly caused by major changes in marine nutrient cycling. Phosphorus (P) limits biological productivity on geologic timescales, thus detailed P geochemistry may provide insight into the role of nutrients in the formation of these organic-rich deposits. P geochemical records that the span the OAE across the Cenomanian-Turonian boundary (CTB, ~94 Ma), known as OAE 2, are rare, and detailed P geochemical records are usually limited to relatively shallow settings. In this study, a sequential extraction (SEDEX) technique was employed to evaluate the sedimentary distribution of P (oxide-associated, authigenic, …

An Empirical Muscle Intracellular Action Potential Model With Multiple Erlang Probability Density Functions Based On A Modified Newton Method, Gyutae Kim, Mohammed M. Ferdjallah, Frederic D. Mckenzie

Computational Modeling & Simulation Engineering Faculty Publications

The convolution of the transmembrane current of an excitable cell and a weighting function generates a single fiber action potential (SFAP) model by using the volume conductor theory. Here, we propose an empirical muscle IAP model with multiple Erlang probability density functions (PDFs) based on a modified Newton method. In addition, we generate SFAPs based on our IAP model and referent sources, and use the peak-to-peak ratios (PPRs) of SFAPs for model verification. Through this verification, we find that the relation between an IAP profile and the PPR of its SFAP is consistent with some previous studies, and our IAP …

Getches Wilkinson Center Newsletter, Winter/Spring 2013, University Of Colorado Boulder. Getches-Wilkinson Center For Natural Resources, Energy, And The Environment

Getches-Wilkinson Center for Natural Resources, Energy, and the Environment Newsletter (2013-)

No abstract provided.

Biomass Accumulation And Carbon Sequestration In Four Different Aged Casuarina Equisetifolia Coastal Shelterbelt Plantations In South China, Faming Wang, Xin Xu, Bi Zou, Zhihua Guo, Zhian Li, Weixing Zhu

Biological Sciences Faculty Scholarship

Thousands of kilometers of shelterbelt plantations of Casuarina equisetifolia have been planted to protect the southeast coastline of China. These plantations also play an important role in the regional carbon (C) cycling. In this study, we examined plant biomass increment and C accumulation in four different aged C. equisetifolia plantations in sandy beaches in South China. The C accumulated in the C. equisetifolia plant biomass increased markedly with stand age. The annual rate of C accumulation in the C. equisetifolia plant biomass during 0-3, 3-6, 6-13 and 13-18 years stage was 2.9, 8.2, 4.2 and 1.0 Mg C ha(-1) yr(-1), …

Liver On A Chip; Engineering The Liver Sinusoid, Young Bok (Abraham) Kang

Faculty Publications - Biomedical, Mechanical, and Civil Engineering

We have developed a novel microfluidic device using poly-dimethylsiloxane (PDMS) microchannels for long term, layered co-culture of primary rat hepatocytes (PRH) and endothelial cells (EC) to mimic the liver sinusoid. Three microfluidic configurations were investigated as shown in Figure 1. In configuration 1, where PRH and rat adrenal medullary endothelial cells (RAMEC) were cocultured with matrigel in layers in a single PDMS microchannel, cells remained viable for 30 days. In configuration 3, where PRH and liver sinusoidal endothelial cells (LSEC) were cultured on the opposite sides of the microporous membrane between two microchannels under static and dynamic conditions, PRH retained …

Notch1 Is Required For Hypoxia-Induced Proliferation, Invasion And Chemoresistance Of T-Cell Acute Lymphoblastic Leukemia Cells, Jie Zou, Peng Li, Fei Lu, Na Liu, Jianjian Dai, Jingjing Ye, Xun Qu, Xiulian Sun, Daoxin Ma, Jino Park, Chunyan Ji

Faculty & Staff Scholarship

Background

Notch1 is a potent regulator known to play an oncogenic role in many malignancies including T-cell acute lymphoblastic leukemia (T-ALL). Tumor hypoxia and increased hypoxia-inducible factor-1α (HIF-1α) activity can act as major stimuli for tumor aggressiveness and progression. Although hypoxia-mediated activation of the Notch1 pathway plays an important role in tumor cell survival and invasiveness, the interaction between HIF-1α and Notch1 has not yet been identified in T-ALL. This study was designed to investigate whether hypoxia activates Notch1 signalling through HIF-1α stabilization and to determine the contribution of hypoxia and HIF-1α to proliferation, invasion and chemoresistance in T-ALL.

Methods …

Phosphorylation Of The Alternative Mrna Splicing Factor 45 (Spf45) By Clk1 Regulates Its Splice Site Utilization, Cell Migration And Invasion, Yuying Liu, Lashardai Conaway, Jennifer R. Bethard, Adnan M. Al-Ayoubi, Amber T. Bradley, Hui Zheng, Scott A. Weed, Scott T. Eblen

Faculty & Staff Scholarship

Alternative mRNA splicing is a mechanism to regulate protein isoform expression and is regulated by alternative splicing factors. The alternative splicing factor 45 (SPF45) is overexpressed in cancer, although few biological effects of SPF45 are known, and few splicing targets have been identified. We previously showed that Extracellular Regulated Kinase 2 (ERK2) phosphorylation of SPF45 regulates cell proliferation and adhesion to fibronectin. In this work, we show that Cdc2-like kinase 1 (Clk1) phosphorylates SPF45 on eight serine residues. Clk1 expression enhanced, whereas Clk1 inhibition reduced, SPF45-induced exon 6 exclusion from Fas mRNA. Mutational analysis of the Clk1 phosphorylation sites on …

Trail And Proteasome Inhibitors Combination Induces A Robust Apoptosis In Human Malignant Pleural Mesothelioma Cells Through Mcl-1 And Akt Protein Cleavages, Bao-Zhu Yuan, Joshua Chapman, Min Ding, Junzhi Wang, Binghua Jiang, Yon Rojanasakul, Steven H. Reynolds

Faculty & Staff Scholarship

Background

Malignant pleural mesothelioma (MPM) is an aggressive malignancy closely associated with asbestos exposure and extremely resistant to current treatments. It exhibits a steady increase in incidence, thus necessitating an urgent development of effective new treatments.

Methods

Proteasome inhibitors (PIs) and TNFα-Related Apoptosis Inducing Ligand (TRAIL), have emerged as promising new anti-MPM agents. To develop effective new treatments, the proapoptotic effects of PIs, MG132 or Bortezomib, and TRAIL were investigated in MPM cell lines NCI-H2052, NCI-H2452 and NCI-H28, which represent three major histological types of human MPM.

Results

Treatment with 0.5-1 μM MG132 alone or 30 ng/mL Bortezomib alone induced …

Serum Perfluorooctanoate (Pfoa) And Perfluorooctane Sulfonate (Pfos) Concentrations And Liver Function Biomarkers In A Population With Elevated Pfoa Exposure, Valentina Gallo, Giovanni Leonard, Bernd Genser, Maria-Jose Lopez-Espinosa, Stephanie J. Frisbee, Lee Karlsson, Alan M. Ducatman, Tony Fletcher

Faculty & Staff Scholarship

Background: Perfluorooctanoate (PFOA) and perfluorooctane sulfonate (PFOS) persist in the environment and are found in relatively high concentrations in animal livers. Studies in humans have reported inconsistent associations between PFOA and liver enzymes.

Objectives: We examined the cross-sectional association between serum PFOA and PFOS concentrations with markers of liver function in adults.

Methods: The C8 Health Project collected data on 69,030 persons; of these, a total of 47,092 adults were included in the present analysis. Linear regression models were fitted for natural log (ln)-transformed values of alanine transaminase (ALT), γ-glutamyltransferase (GGT), and direct bilirubin on PFOA, PFOS, and potential confounders. …

Currencies Of Mutualisms: Sources Of Alkaloid Genes In Vertically Transmitted Epichloae, Christopher L. Schardl, Carolyn A. Young, Juan Pan, Simona Florea, Johanna E. Takach, Daniel G. Panaccione, Mark L. Farman, Jennifer S. Webb, Jolanta Jaromczyk, Nikki D. Charlton, Padmaja Nagabhyru, Li Chen, Chong Shi, Adrian Leuchtmann

Faculty & Staff Scholarship

The epichloae (Epichloë and Neotyphodium species), a monophyletic group of fungi in the family Clavicipitaceae, are systemic symbionts of cool-season grasses (Poaceae subfamily Poöideae). Most epichloae are vertically transmitted in seeds (endophytes), and most produce alkaloids that attack nervous systems of potential herbivores. These protective metabolites include ergot alkaloids and indole-diterpenes (tremorgens), which are active in vertebrate systems, and lolines and peramine, which are more specific against invertebrates. Several Epichloë species have been described which are sexual and capable of horizontal transmission, and most are vertically transmissible also. Asexual epichloae are mainly or exclusively vertically transmitted, and many are interspecific …

Odor Detection In Manduca Sexta Is Optimized When Odor Stimuli Are Pulsed At A Frequency Matching The Wing Beat During Flight, Kevin C. Daly, Faizan Kalwar, Mandy Hatfield, Erich Staudacher, Samual P. Bradley

Faculty & Staff Scholarship

Sensory systems sample the external world actively, within the context of self-motion induced disturbances. Mammals sample olfactory cues within the context of respiratory cycles and have adapted to process olfactory information within the time frame of a single sniff cycle. In plume tracking insects, it remains unknown whether olfactory processing is adapted to wing beating, which causes similar physical effects as sniffing. To explore this we first characterized the physical properties of our odor delivery system using hotwire anemometry and photo ionization detection, which confirmed that odor stimuli were temporally structured. Electroantennograms confirmed that pulse trains were tracked physiologically. Next, …

Cortactin Is A Substrate Of Activated Cdc42-Associated Kinase 1 (Ack1) During Ligand-Induced Epidermal Growth Factor Receptor Downregulation, Laura C. Kelley, Scott A. Weed

Faculty & Staff Scholarship

Background

Epidermal growth factor receptor (EGFR) internalization following ligand binding controls EGFR downstream pathway signaling activity. Internalized EGFR is poly-ubiquitinated by Cbl to promote lysosome-mediated degradation and signal downregulation. ACK1 is a non-receptor tyrosine kinase that interacts with ubiquitinated EGFR to facilitate EGFR degradation. Dynamic reorganization of the cortical actin cytoskeleton controlled by the actin related protein (Arp)2/3 complex is important in regulating EGFR endocytosis and vesicle trafficking. How ACK1-mediated EGFR internalization cooperates with Arp2/3-based actin dynamics during EGFR downregulation is unclear.

Methodology/Principal Findings

Here we show that ACK1 directly binds and phosphorylates the Arp2/3 regulatory protein cortactin, potentially providing …

Identification And Characterization Of Micrornas In White And Brown Alpaca Skin, Xue Tian, Junbing Jiang, Ruiwen Fan, Haidong Wang, Xialong Meng, Xiaoyan He, Junping He, Hongquan Li, Jianjun Geng, Xiuju Yu, Yunfei Song, Danli Zhang, Jianbo Yao, George W. Smith, Changsheng Dong

Faculty & Staff Scholarship

Background

MicroRNAs (miRNAs) are small, non-coding 21–25 nt RNA molecules that play an important role in regulating gene expression. Little is known about the expression profiles and functions of miRNAs in skin and their role in pigmentation. Alpacas have more than 22 natural coat colors, more than any other fiber producing species. To better understand the role of miRNAs in control of coat color we performed a comprehensive analysis of miRNA expression profiles in skin of white versus brown alpacas.

Results

Two small RNA libraries from white alpaca (WA) and brown alpaca (BA) skin were sequenced with the aid of …

The P53 Codon 72 Pro/Pro Genotype May Be Associated With Initial Central Visual Field Defects In Caucasians With Primary Open Angle Glaucoma, Janey L. Wiggs, Alex W. Hewitt, Bao J. Fan, Dan Y. Wang, Dayse R. Figueiredo Sena, Colm O'Brien, Anthony Realini, Jamie E. Craig, David P. Dimasi, David A. Mackey, Jonathan L. Haines, Louis R. Pasquale

Faculty & Staff Scholarship

Background

Loss of vision in glaucoma is due to apoptotic retinal ganglion cell loss. While p53 modulates apoptosis, gene association studies between p53 variants and glaucoma have been inconsistent. In this study we evaluate the association between a p53 variant functionally known to influence apoptosis (codon 72 Pro/Arg) and the subset of primary open angle glaucoma (POAG) patients with early loss of central visual field.

Methods

Genotypes for the p53 codon 72 polymorphism (Pro/Arg) were obtained for 264 POAG patients and 400 controls from the U.S. and in replication studies for 308 POAG patients and 178 controls from Australia (GIST). …

Characterization Of The Rainbow Trout Egg Microrna Transcriptome, Hao Ma, Mark Hostuttler, Hairong Wei, Caird E. Rexroad, Jianbo Yao

Faculty & Staff Scholarship

MicroRNAs (miRNAs) are a class of endogenous small non-coding RNA molecules that regulate post-transcriptional expression of target genes and play important roles in animal development. The objectives of this study were to characterize the egg miRNA transcriptome and identify novel egg-predominant miRNAs in rainbow trout. Small RNAs isolated from mature unfertilized rainbow trout eggs were subjected to deep sequencing using an Illumina Genome Analyzer. The massive sequencing produced 24,621,741 quality reads, among which, 266 known miRNAs were identified and 230 putatively novel miRNAs were predicted. The most abundantly known miRNAs are let-7 and miR-21, accounting for 24.06% and 18.71% of …

Partial Reconstruction Of The Ergot Alkaloid Pathway By Heterologous Gene Expression In Aspergillus Nidulans, Katy L. Ryan, Christopher T. Moore, Daniel G. Panaccione

Faculty & Staff Scholarship

Ergot alkaloids are pharmaceutically and agriculturally important secondary metabolites produced by several species of fungi. Ergot alkaloid pathways vary among different fungal lineages, but the pathway intermediate chanoclavine-I is evolutionarily conserved among ergot alkaloid producers. At least four genes, dmaW, easF, easE, and easC, are necessary for pathway steps prior to chanoclavine-I; however, the sufficiency of these genes for chanoclavine-I synthesis has not been established. A fragment of genomic DNA containing dmaW, easF, easE, and easC was amplified from the human-pathogenic, ergot alkaloid-producing fungus Aspergillus fumigatus and transformed into Aspergillus nidulans, a model fungus that does not contain any of …

Genome-Wide Association Scan Of Dental Caries In The Permanent Dentition, Xiaojing Wang, John R. Schaffer, Zhen Zeng, Ferdouse Begum, Alexandre R. Vieira, Jacqueline Noel, Ida Anjomshoaa, Karen T. Cuenco, Myoung-Keun Lee, James Beck, Eric Boerwinkle, Marilyn C. Cornelis, Frank B. Hu, David R. Crosslin, Cathy C. Laurie, Sarah C. Nelson, Kimberly F. Doheny, Elizabeth W. Pugh, Deborah E. Polk, Robert J. Weyant, Richard Crout, Daniel W. Mcneil, Daniel E. Weeks, Eleanor Feingold, Mary L. Marazita

Faculty & Staff Scholarship

Background

Over 90% of adults aged 20 years or older with permanent teeth have suffered from dental caries leading to pain, infection, or even tooth loss. Although caries prevalence has decreased over the past decade, there are still about 23% of dentate adults who have untreated carious lesions in the US. Dental caries is a complex disorder affected by both individual susceptibility and environmental factors. Approximately 35-55% of caries phenotypic variation in the permanent dentition is attributable to genes, though few specific caries genes have been identified. Therefore, we conducted the first genome-wide association study (GWAS) to identify genes affecting …

Cloning And Characterization Of A Novel Oocyte-Specific Gene Encoding An F-Box Protein In Rainbow Trout (Oncorhynchus Mykiss), Lei Wang, Swamy K. Tripurani, Warapond Wanna, Caird E. Rexroad, Jianbo Yao

Faculty & Staff Scholarship

Background

Oocyte-specific genes play critical roles in oogenesis, folliculogenesis and early embryonic development. The objectives of this study were to characterize the expression of a novel oocyte-specific gene encoding an F-box protein during ovarian development in rainbow trout, and identify its potential interacting partners in rainbow trout oocytes.

Methods

Through analysis of expressed sequence tags (ESTs) from a rainbow trout oocyte cDNA library, a novel transcript represented by ESTs only from the oocyte library was identified. The complete cDNA sequence for the novel gene (named fbxoo) was obtained by assembling sequences from an EST clone and a 5′RACE product. …

Pulmonary Exposure To Single-Walled Carbon Nanotubes Does Not Affect The Early Immune Response Against Toxoplasma Gondii, Loinda Swedin, Romanico Arrighi, Britta Anderson-Willman, Ashley Murray, Yunying Chen, Mikael C I Karlsson, Susanna K. Georen, Alexey V. Tkach, Anna A. Shvedova, Bengt Fadeel, Antonio Barragan, Annika Scheynius

Faculty & Staff Scholarship

Background

Single-walled carbon nanotubes (SWCNT) trigger pronounced inflammation and fibrosis in the lungs of mice following administration via pharyngeal aspiration or inhalation. Human exposure to SWCNT in an occupational setting may occur in conjunction with infections and this could yield enhanced or suppressed responses to the offending agent. Here, we studied whether the sequential exposure to SWCNT via pharyngeal aspiration and infection of mice with the ubiquitous intracellular parasite Toxoplasma gondii would impact on the immune response of the host against the parasite.

Methods

C57BL/6 mice were pre-exposed by pharyngeal administration of SWCNT (80 + 80 μg/mouse) for two consecutive …

Metastatic Mtln3 And Non-Metastatic Mtc Adenocarcinoma Cells Can Be Differentiated By Pseudomonas Aeruginosa, Matthew J. Novotny, Dacie R. Bridge, Karen H. Martin, Scott A. Weed, Robert B. Wysolmerski, Joan C. Olson

Faculty & Staff Scholarship

Cancer patients are known to be highly susceptible to Pseudomonas aeruginosa (Pa) infection, but it remains unknown whether alterations at the tumor cell level can contribute to infection. This study explored how cellular changes associated with tumor metastasis influence Pa infection using highly metastatic MTLn3 cells and non-metastatic MTC cells as cell culture models. MTLn3 cells were found to be more sensitive to Pa infection than MTC cells based on increased translocation of the type III secretion effector, ExoS, into MTLn3 cells. Subsequent studies found that higher levels of ExoS translocation into MTLn3 cells related to Pa entry and secretion …

Pyrvinium Pamoate Changes Alternative Splicing Of The Serotonin Receptor 2c By Influencing Its Rna Structure, Manli Shen, Stanislav Bellaousov, Michael Hiller, Pierre De La Grange, Trevor P. Creamer, Orit Malina, Ruth Sperling, David H. Mathews, Peter Stoilov, Stefan Stamm

Faculty & Staff Scholarship

The serotonin receptor 2C plays a central role in mood and appetite control. It undergoes pre-mRNA editing as well as alternative splicing. The RNA editing suggests that the pre-mRNA forms a stable secondary structure in vivo. To identify substances that promote alternative exons inclusion, we set up a high-throughput screen and identified pyrvinium pamoate as a drug-promoting exon inclusion without editing. Circular dichroism spectroscopy indicates that pyrvinium pamoate binds directly to the pre-mRNA and changes its structure. SHAPE (selective 2′-hydroxyl acylation analysed by primer extension) assays show that part of the regulated 5′-splice site forms intramolecular base pairs that are …

Predicting Whole Genome Protein Interaction Networks From Primary Sequence Data In Model And Non-Model Organisms Using Ents, Eli Rodgers-Melnick, Mark Culp, Stephen P. Difazio

Faculty & Staff Scholarship

Background

The large-scale identification of physical protein-protein interactions (PPIs) is an important step toward understanding how biological networks evolve and generate emergent phenotypes. However, experimental identification of PPIs is a laborious and error-prone process, and current methods of PPI prediction tend to be highly conservative or require large amounts of functional data that may not be available for newly-sequenced organisms.

Results

In this study we demonstrate a random-forest based technique, ENTS, for the computational prediction of protein-protein interactions based only on primary sequence data. Our approach is able to efficiently predict interactions on a whole-genome scale for any eukaryotic organism, …

Identification Of Functionally Distinct Na-Hco3 Co-Transporters In Colon, Christian Barmeyer, Jeff H. Ye, Carol Soroka, Peter Geibel, Lukas M. Hingsammer, Laurence Weitgasser, Danny Atway, John P. Geibel, Henry J. Binder, Vazhaikkurichi M. Rajendran

Faculty & Staff Scholarship

Na-HCO3 cotransport (NBC) regulates intracellular pH (pHi) and HCO3 secretion in rat colon. NBC has been characterized as a 5,5′-diisothiocyanato-2-2′-stilbene (DIDS)-sensitive transporter in several tissues, while the colonic NBC is sensitive to both amiloride and DIDS. In addition, the colonic NBC has been identified as critical for pHi regulation as it is activated by intravesicular acid pH. Molecular studies have identified several characteristically distinct NBC isoforms [i.e. electrogenic (NBCe) and electroneutral (NBCn)] that exhibit tissue specific expression. This study was initiated to establish the molecular identity and specific function of NBC isoforms in rat colon. Northern blot and reverse transcriptase …

Impairment Of Coronary Arteriolar Endothelium-Dependent Dilation After Multi-Walled Carbon Nanotube Inhalation: A Time-Course Study, Phoebe A. Stapleton, Valerie C. Minarchick, Amy M. Cumpston, Walter Mckinney, Bean T. Chen, Tina M. Sager, David G. Frazer, Robert R. Mercer, James Scabilloni, Michael E. Andrew, Vincent Castranova, Timothy R. Nurkiewicz

Faculty & Staff Scholarship

Engineered nanomaterials have been developed for widespread applications due to many highly unique and desirable characteristics. The purpose of this study was to assess pulmonary inflammation and subepicardial arteriolar reactivity in response to multi-walled carbon nanotube (MWCNT) inhalation and evaluate the time course of vascular alterations. Rats were exposed to MWCNT aerosols producing pulmonary deposition. Pulmonary inflammation via bronchoalveolar lavage and MWCNT translocation from the lungs to systemic organs was evident 24 h post-inhalation. Coronary arterioles were evaluated 24–168 h post-exposure to determine microvascular response to changes in transmural pressure, endothelium-dependent and -independent reactivity. Myogenic responsiveness, vascular smooth muscle reactivity …

Interlaboratory Evaluation Of In Vitro Cytotoxicity And Inflammatory Responses To Engineered Nanomaterials: The Niehs Nano Go Consortium, Tian Xia, Raymond F. Hamilton, James C. Bonner, Edward D. Crandall, Alison Elder, Farnoosh Fazlollahi, Teri A. Girtsman, Kwang Kim, Somenath Mitra, Susana A. Ntim, Galya Orr, Mani Tagmount, Alexia J. Taylor, Donatello Telesca, Ana Tolic, Christopher D. Vulpe, Andrea J. Walker, Xiang Wang, Frank A. Witzmann, Nianqiang Wu, Yumei Xie, Jeffery I. Zink, Andre Nel, Andrij Holian

Faculty & Staff Scholarship

Background: Differences in interlaboratory research protocols contribute to the conflicting data in the literature regarding engineered nanomaterial (ENM) bioactivity.

Objectives: Grantees of a National Institute of Health Sciences (NIEHS)-funded consortium program performed two phases of in vitro testing with selected ENMs in an effort to identify and minimize sources of variability.

Methods: Consortium program participants (CPPs) conducted ENM bioactivity evaluations on zinc oxide (ZnO), three forms of titanium dioxide (TiO2), and three forms of multiwalled carbon nanotubes (MWCNTs). In addition, CPPs performed bioassays using three mammalian cell lines (BEAS-2B, RLE-6TN, and THP-1) selected in order to cover two different species …

Comparative Efficacy Of Tandem Autologous Versus Autologous Followed By Allogeneic Hematopoietic Cell Transplantation In Patients With Newly Diagnosed Multiple Myeloma: A Systematic Review And Meta-Analysis Of Randomized Controlled Trials, Mohamed A. Kharfan-Dabaja, Mehdi Hamadani, Tea Reljic, Taiga Nishihori, William Bensinger, Benjamin Djulbegovic, Ambuj Kumar

Faculty & Staff Scholarship

Background

Despite advances in understanding of clinical, genetic, and molecular aspects of multiple myeloma (MM) and availability of more effective therapies, MM remains incurable. The autologous-allogeneic (auto-allo) hematopoietic cell transplantation (HCT) strategy is based on combining cytoreduction from high-dose (chemo- or chemoradio)-therapy with adoptive immunotherapy. However, conflicting results have been reported when an auto-allo HCT approach is compared to tandem autologous (auto-auto) HCT. A previously published meta-analysis has been reported; however, it suffers from serious methodological flaws.

Methods

A systematic search identified 152 publications, of which five studies (enrolling 1538 patients) met inclusion criteria. All studies eligible for inclusion utilized …