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The Role Of Oxidative Stress In The Pathogenesis Of Epithelial Ovarian Cancer, Nicole Marie King

Wayne State University Dissertations

Ovarian cancer is the leading cause of death from gynecologic malignancies yet the underlying pathophysiology is not clearly established. The disease is usually diagnosed in the advanced stage and carries a poor prognosis. The 5-year survival rate is greater than 75% if diagnosis of the cancer occurs at an early stage; however, this rate drops to 20% when the tumor has spread beyond its origin. Thus, a method for early detection is critically needed, which can help prolong, or even save lives. Currently, an effective screening test for ovarian cancer is lacking. Many tests have been evaluated but have been …

A Juvenile Perspective: What Affects Attitudes Of African American, Hispanic, And White Youth Toward The Police., Rodney Lamon Lake

Wayne State University Dissertations

Race is one of the most powerful variables explaining the public's attitudes toward the police. The majority of studies on race and attitudes toward the police have explored differences between African Americans and Whites. The emphasis of previous research on Black-White comparisons has left unanswered many questions about minority group differences in attitudes toward the police, especially differences between Latinos and African Americans. With the usage of secondary data ("Outcome Evaluation of the Teens, Crime, and the Community/Community Works (TCC/CW) Training Program in Nine Cities across Four States, 2004-2005), this study determined which independent variables (race, age, gender, class, self …

Characterization Of High-Voltage-Activated Calcium Channels In Retinal Bipolar Cells, Qi Lu

Wayne State University Dissertations

Retinal bipolar cells, conveying visual information from photoreceptors to ganglion cells, segregate visual information into multiple parallel pathways through their diversified cell types and physiological properties. Voltage-gated Ca²⁺ channels could be particularly important underlying the diversified physiological properties of different BCs. In this dissertation, I investigated the high-voltage-activated (HVA) calcium current in retinal bipolar cells in mice. In the first part of my dissertation, I characterized multiple bipolar cell-expressing GFP and/or Cre transgenic mouse lines. In the second part of my dissertation, by performing whole-cell patch-clamp recordings, I examined the electrophysiological properties of HVA calcium currents among CBCs and …

The Drosophila Interactions Database: Integrating The Interactome And Transcriptome, Thilakam Murali

Wayne State University Dissertations

In this thesis I describe the integration of heterogeneous interaction data for Drosophila into DroID, the Drosophilainteractions database, making it a one-stop public resource for interaction data. I have also made it possible to filter the interaction data using gene expression data to generate context-relevant networks making DroID a one-of-a kind resource for biologists. In the two years since the upgraded DroID has been available, several studies have used the heterogeneous interaction data in DroID to advance our understanding of Drosophila biology thus validating the need for such a resource for biologists. In addition to this, I have identified …

A Cognitively Enabling Approach: Cognitive Diversity In Composition Studies, Crystal June Starkey

Wayne State University Dissertations

ABSTRACT

A COGNITIVELY ENABLING APPROACH: COGNITIVE DIVERSITY IN COMPOSITION STUDIES.

by

CRYSTAL STARKEY

August 2013

Advisor: Dr. Jeff Pruchnic

Major: English (Composition Studies)

Degree: Doctor of Philosophy

My dissertation project investigates the rhetoric of cognitive disability in relation to the theory and teaching of rhetoric and composition in entry-level writing courses. Students with Autism Spectrum Disorder (ASD), who frequently struggle with generalizing as well as feeling and expressing emotions, are often perceived to have a decreased intellectual ability and thus not often considered part of the traditional student body of higher education classrooms. Yet, as ASD diagnostic criteria has changed …

Impact Of Cryoablation On Tumor Immunity, Jesse Veenstra

Wayne State University Dissertations

Background and Objectives: Percutaneous cryoablation is a minimally invasive procedure for tumor debulking, which has the potential to initiate or amplify tumor immunity through the release of tumor-associated antigens and endogenous danger signals. However, enhanced immunity is rarely observed in treated patients, suggesting the need for mechanistic analysis. The goal is to determine how cryoablation affects tumor specific immunity and if the response can be improved through exogenous TLR9 stimulation.

Methodology: We evaluated anti-Her2/neu immunity following cryoablation in wt BALB/c and tolerant NeuT mice inoculated with neu or Her2 expressing mammary tumors TUBO and D2F2/E2 respectively. Mice were treated with …

Music And Movement Share A Dynamic Structure That Supports Universal Expressions Of Emotion, Beau Sievers, Larry Polansky, Michael Casey, Thalia Wheatley

Dartmouth Scholarship

Music moves us. Its kinetic power is the foundation of human behaviors as diverse as dance, romance, lullabies, and the military march. Despite its significance, the music-movement relationship is poorly understood. We present an empirical method for testing whether music and movement share a common structure that affords equivalent and universal emotional expressions. Our method uses a computer program that can generate matching examples of music and movement from a single set of features: rate, jitter (regularity of rate), direction, step size, and dissonance/visual spikiness. We applied our method in two experiments, one in the United States and another in …

Roles Of Neuregulin1 In Neuromuscular Junction Development, Jiajing Wang

Wayne State University Dissertations

Neuromuscular junction (NMJ) development is a multistep process mediated by coordinated interactions between nerve terminals, target muscles, and peri-synaptic glial cells, and thus requires reciprocal signals derived from every cell type. Neuregulin1s (NRG1s) are a family of predominantly neuronal growth and differentiation factors that are important for many aspects of nervous system development. In this thesis, both the effects of NRG1 on NMJ development and reciprocal effects of neurotrophic factors on NRG1 expression were studied as a means to define the complex regulatory communication at the NMJ. Using the chicken embryo as a model, methods were developed to study the …

Towards Personalized Medicine Using Systems Biology And Machine Learning, Calin Voichita

Wayne State University Dissertations

The rate of acquiring biological data has greatly surpassed our ability to interpret it. At the same time, we have started to understand that evolution of many diseases such as cancer, are the results of the interplay between the disease itself and the immune system of the host. It is now well accepted that cancer is not a single disease, but a “complex collection of distinct genetic diseases united by common hallmarks”. Understanding the differences between such disease subtypes is key not only in providing adequate treatments for known subtypes but also identifying new ones. These unforeseen disease subtypes are …

Gleevec, An Abl Family Inhibitor, Produces A Profound Change In Cell Shape And Migration, Zaozao Chen, Elizabeth Lessey, Matthew E. Berginski, Li Cao, Jonathan Li, Xavier Trepat, Michelle Itano, Shawn M. Gomez, Maryna Kapustina, Cai Huang, Keith Burridge, George Truskey, Ken Jacobson

Markey Cancer Center Faculty Publications

The issue of how contractility and adhesion are related to cell shape and migration pattern remains largely unresolved. In this paper we report that Gleevec (Imatinib), an Abl family kinase inhibitor, produces a profound change in the shape and migration of rat bladder tumor cells (NBTII) plated on collagen-coated substrates. Cells treated with Gleevec adopt a highly spread D-shape and migrate more rapidly with greater persistence. Accompanying this more spread state is an increase in integrin-mediated adhesion coupled with increases in the size and number of discrete adhesions. In addition, both total internal reflection fluorescence microscopy (TIRFM) and interference reflection …

Phenotypic Characterization Of Peripheral Osteoclast Precursors Their Lineage Relation To Macrophages And Dendritic Cells And Their Population Dynamics Influenced By Parathyroid Hormone And Inflammatory Signals., Christian E. Jacome-Galarza

Doctoral Dissertations

Osteoclasts are unique multinuclear cells that are highly specialized for resorbing bone tissue and therefore play crucial roles in bone remodeling. The identification of bona fide populations of osteoclast precursors (OCPs) is crucial to understand their biology in homeostatic and pathological conditions. We identified a bone marrow precursor with high osteoclastogenic activity (Jacquin et al., 2006) and extended these findings to test their clonogenic potential and bone resorptive capacity at the single cell level. We also identified the phenotype of peripheral blood and spleen OCPs. In chapter III, we showed that bone marrow and peripheral OCPs gave rise to macrophages …

Microstructural Abnormalities In Striatial And Medial-Temporal Tracts In Children With A History Of Early Severe Deprivation: A Diffusion Tensor Imaging Study, Amy Veenstra

Wayne State University Dissertations

The purpose of this study was to determine if early social deprivation led to different patterns of frontal and/or medial-temporal cortical connectivity in internationally adopted children and if these patterns of cortical connectivity were related to specific white matter tract or behavioral differences. Based on theories of brain development, it was expected that environmental factors would impact children's tract based differences and behavioral differences. A sample of 30 internationally adopted children and 12 comparison children participated in functional and structural imaging and a neuropsychological evaluation. For internationally adopted children frontal-cortical connectivity showed patterns of diffuse and low connectivity, whereas medial-temporal …

Comparison Of The Therapeutic Effects Of Human And Mouse Adipose-Derived Stem Cells In A Murine Model Of Lipopolysaccharide-Induced Acute Lung Injury, Shijia Zhang, Svitlana Danchuk, Kathleen M. P. Imhof, Julie A. Semon, Brittni A. Scruggs, Ryan W. Bonvillain, Amy L. Strong, Jeffrey M. Gimble, Aline M. Betancourt, For Full List Of Authors, See Publisher's Website.

Biological Sciences Faculty Research & Creative Works

Introduction. Adipose-derived stem cells (ASCs) have emerged as important regulators of inflammatory/immune responses in vitro and in vivo and represent attractive candidates for cell-based therapies for diseases that involve excessive inflammation. Acute lung injury (ALI) is an inflammatory condition for which treatment is mainly supportive due to lack of effective therapies. In this study, the therapeutic effects of ASC-based therapy were assessed in vivo by comparison of the anti-inflammatory properties of both human and murine ASCs in a mouse model of lipopolysaccharide (LPS)-induced ALI.

Methods. Human ASCs (hASCs) or mouse ASCs (mASCs) were delivered to C57Bl/6 mice (7.5 x 10 …

Obesity Associated Alterations In The Biology Of Adipose Stem Cells Mediate Enhanced Tumorigenesis By Estrogen Dependent Pathways, Amy L. Strong, Thomas A. Strong, Lyndsay V. Rhodes, Julie A. Semon, Xiujuan Zhang, Zhenzhen Shi, Shijia Zhang, Jeffrey M. Gimble, Matthew E. Burow, Bruce A. Bunnell

Biological Sciences Faculty Research & Creative Works

Introduction: Obesity has been associated with increased incidence and mortality of breast cancer. While the precise correlation between obesity and breast cancer remains to be determined, recent studies suggest that adipose tissue and adipose stem cells (ASCs) influence breast cancer tumorigenesis and tumor progression.

Methods: Breast cancer cells lines were co-cultured with ASCs (n = 24), categorized based on tissue site of origin and body mass index (BMI), and assessed for enhanced proliferation, alterations in gene expression profile with PCR arrays, and enhanced tumorigenesis in immunocompromised mice. The gene expression profile of ASCs was assess with PCR arrays and qRT-PCR …

Analysis Of Feeding Behavior Of Group Housed Growing–Finishing Pigs, T.M. Brown-Brandl, G.A. Rohrer, R.A. Eigenberg

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Feeding behavior and time spent eating contains valuable information that can be used for managing livestock, identifying sick animals, and determining genetic differences within a herd. Individual animal feeding behavior, in a commercial-sized pen, was recorded using radio-frequency identification (RFID) technology and a series of multiplexers. Data were collected on 960 pigs (mixed barrows, 406 and gilts, 600) over 4 grow-out periods. The animals entered the facility at 24.6 ± 5.4 kg (mean ± standard deviation) at approximately 65 days of age and exited the facility at 101.4 ± 13.8 kg (between 116 and 133 days later). Time spent at …

Small Ruminant Lentivirus Genetic Subgroups Associate With Sheep Tmem154 Genotypes, Lucia H. Sider, Michael P. Heaton, Carol G. Chitko-Mckown, Greg P. Harhay, Timothy P. L. Smith, Kreg A. Leymaster, William W. Laegreid, Michael L. Clawson

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Small ruminant lentiviruses (SRLVs) are prevalent in North American sheep and a major cause of production losses for the U.S. sheep industry. Sheep susceptibility to SRLV infection is influenced by genetic variation within the ovine transmembrane 154 gene (TMEM154). Animals with either of two distinct TMEM154 haplotypes that both encode glutamate at position 35 of the protein (E35) are at greater risk of SRLV infection than those homozygous with a lysine (K35) haplotype. Prior to this study, it was unknown if TMEM154 associations with infection are influenced by SRLV genetic subgroups. Accordingly, our goals were to characterize SRLVs …

Fy 2013 Annual Report, Tennessee. Division Of Forestry, Tennessee. Department Of Agriculture

Forestry Annual Reports

This annual report presents an overview of Tennessee Forestry Commission programs and management.

New Clone Particle Swarm Optimization-Based Particle Filter Algorithm And Its Application, Yuming Bo, Zhimin Chen, Jie Zhang, Jianliang Zhu

Applied Mathematics & Information Sciences

A new particle filter algorithm based on new Clone particle swarm optimization（NCPSO-PF） is presented in this paper in order to solve the problem of low precision and complicated calculation of particle filter based on particle swarm optimization algorithm(PSO-PF). The algorithm enables the particles to fit the environment better and then reach the goal of global optimization through orthogonal initialization, clonal selection and local searching of self-learning. Finally different models are used for simulation experiment and the results indicate that this new algorithm improves the operation speed and precision of practical engineering application.

Tricarballylic Ester Formation During Biosynthesis Of Fumonisin Mycotoxins In Fusarium Verticillioides, Yaoyao Li, Lili Lou, Ronald Cerny, Robert A. E. Butchko, Robert H. Proctor, Yuemao Shen, Liangcheng Du

Ronald Cerny Publications

Fumonisins are agriculturally important mycotoxins produced by the maize pathogen Fusarium verticillioides. The chemical structure of fumonisins contains two tricarballylic esters, which are rare structural moieties and important for toxicity. The mechanism for the tricarballylic ester formation is not well understood. FUM7 gene of F. verticillioides was predicted to encode a dehydrogenase/reductase, and when it was deleted, the mutant produced tetradehydro fumonisins (DH₄–FB). MS and NMR analysis of DH₄–FB₁ indicated that the esters consist of aconitate with a 3′-alkene function, rather than a 2′-alkene function. Interestingly, the purified DH₄–FB₁ eventually yielded …

Chromogenic Agar Medium For Detection And Isolation Of Escherichia Coli Serogroups O26, O45, O103, O111, O121, And O145 From Fresh Beef And Cattle Feces, Norasak Kalchayanand, Terrance M. Arthur, Joseph M. Bosilevac, James E. Wells, Tommy L. Wheeler

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Non-O157 Shiga toxin–producing Escherichia coli (STEC) strains are clinically important foodborne pathogens. Unlike E. coli O157:H7, these foodborne pathogens have no unique biochemical characteristics to readily distinguish them from other E. coli strains growing on plating media. In this study, a chromogenic agar medium was developed in order to differentiate among non-O157 STEC strains of serogroups O26, O45, O103, O111, O121, and O145 on a single agar medium. The ability of this chromogenic agar medium to select and distinguish among these pathogens is based on a combination of utilization of carbohydrates, β-galactosidase activity, and resistance to selective agents. The agar …

A Comparison Between Male Perpetrators Of Intimate Partner Violence And Child Sexual Abuse: A Feminist Perspective, Christelle Schafer

Electronic Theses and Dissertations

The purpose of this study was to explore whether attributes of sex role identity and gender role stress differed between perpetrators of child sexual abuse (CSA) and perpetrators of intimate partner violence (IPV). The primary research question posed in the research sought to determine if participants’ attitudes on gender role stereotyping or gender role stress were significantly different between perpetrators of CSA and perpetrators of IPV. Participants in this study were a convenience sample of adult males with histories of CSA and IPV from two different outpatient counseling programs. Participants completed the Bem Sex Role Inventory-Short Form (BSRI-SF) and the …

Cholera Toxin Activates The Unfolded Protein Response Through An Adenylate Cyclase-Independent Mechanism, Neyda Vanbennekom

Electronic Theses and Dissertations

Cholera toxin (CT) is a bacterial protein toxin responsible for the gastrointestinal disease known as cholera. CT stimulates its own entry into intestinal cells after binding to cell surface receptors. Once internalized, CT is delivered via vesicle-mediated transport to the endoplasmic reticulum (ER), where the CTA1 subunit dissociates from the rest of the toxin and is exported (or translocated) into the cytosol. CTA1 translocates from the ER lumen into the host cytosol by exploiting a host quality control mechanism called ER-associated degradation (ERAD) that facilitates the translocation of misfolded proteins into the cytosol for degradation. Cytosolic CTA1, however, escapes this …

Town Of Dunbarton, New Hampshire For The Fiscal Year Ending December 31, 2012., Dunbarton Town Representatives

Dunbarton, NH Annual Reports

This is an annual report containing vital statistics for a town/city in the state of New Hampshire.

Current Thoughts On Maternal Nutrition And Fetal Programming Of The Metabolic Syndrome, B. Brenseke, M. R. Prater, J. Bahamonde, J. C. Gutierrez

Osteopathic Medicine, Jerry M. Wallace School of

No abstract provided.

2013 Nebraska Water Monitoring Programs Report, Marty Link, Joe Kenning

Nebraska Department of Environmental Quality: Reports

The Nebraska Department of Environmental Quality (NDEQ) is charged with monitoring, assessing, and to the extent possible, managing the state’s water resources. The purpose of this work is to protect and maintain good quality water and encourage or execute activities to improve poor water quality. Monitoring is done on the over 16,000 miles of flowing rivers and streams, our greater than 148,000 acres of surface water in lakes and reservoirs, and the vast storage of groundwater in Nebraska’s aquifers.

Isolation And Characterization Of The Grain Mold Fungi Cochliobolus And Alternaria Spp. From Sorghum Using Semiselective Media And Dna Sequence Analyses, Deanna L. Funnell-Harris, Louis K. Prom, Jeffrey F. Pedersen

Department of Plant Pathology: Faculty Publications

Mold diseases, caused by fungal complexes including Alternaria, Cochliobolus, and Fusarium species, limit sorghum grain production. Media were tested by plating Fusarium thapsinum, Alternaria sp., and Curvularia lunata, individually and competitively. Dichloran chloramphenicol rose bengal (DRBC) and modified V8 juice (ModV8) agars, found to be useful, were compared with commonly used agar media, dichloran chloramphenicol peptone (DCPA) and pentachloronitrobenzene (PCNB). Radial growth, starting with mycelia or single-conidia and hyphal tips, demonstrated an effect of media. For isolation of grain fungi, DRBC and ModV8 were similar or superior to DCPA and PCNB. When seedlings were inoculated with conidia of C. lunata, …

Occurrence And Distribution Of Triticum Mosaic Virus In The Central Great Plains, E. Byamukama, D. L. Seifers, G. L. Hein, E. De Wolf, N. A. Tisserat, M. A. C. Langham, L. E. Osborne, A. Timmerman, S. N. Wegulo

Department of Plant Pathology: Faculty Publications

Wheat curl mite (WCM)-transmitted viruses—namely, Wheat streak mosaic virus (WSMV), Triticum mosaic virus (TriMV), and the High Plains virus (HPV)—are three of the wheat-infecting viruses in the central Great Plains of the United States. TriMV is newly discovered and its prevalence and incidence are largely unknown. Field surveys were carried out in Colorado, Kansas, Nebraska, and South Dakota in spring and fall 2010 and 2011 to determine TriMV prevalence and incidence and the frequency of TriMV co-infection with WSMV or HPV in winter wheat. WSMV was the most prevalent and was detected in 83% of 185 season–counties (= s-counties), 73% …

Pacs Bulletin 2013-2014, Program In Audiology And Communication Sciences

PACS News

No abstract provided.

A Proposal For A Problem-Driven Mathematics Curriculum Framework, Judith S. Zawojewski, Marta T. Magiera, Richard Lesh

The Mathematics Enthusiast

A framework for a problem-driven mathematics curriculum is proposed, grounded in the assumption that students learn mathematics while engaged in complex problem-solving activity. The framework is envisioned as a dynamic technologicallydriven multi-dimensional representation that can highlight the nature of the curriculum (e.g., revealing the relationship among modeling, conceptual, and procedural knowledge), can be used for programmatic, classroom and individual assessment, and can be easily revised to reflect ongoing changes in disciplinary knowledge development and important applications of mathematics. The discussion prompts ideas and questions for future development of the envisioned software needed to enact such a framework.

Improved Detection Of Common Variants Associated With Schizophrenia And Bipolar Disorder Using Pleiotropy-Informed Conditional False Discovery Rate, Ole A. Andreassen, Wesley K. Thompson, Andrew J. Schork, Stephan Ripke, Morten Mattingsdal, John R. Kelsoe, Kenneth S. Kendler, Michael C. O'Donovan, Dan Rujescu, Thomas Werge, Pamela Sklar, J. Cooper Roddey, Chi-Hua Chen, Linda Mcevoy, Rahul S. Desikan, Srdjan Djurovic, Anders M. Dale

Psychiatry Publications

Several lines of evidence suggest that genome-wide association studies (GWAS) have the potential to explain more of the “missing heritability” of common complex phenotypes. However, reliable methods to identify a larger proportion of single nucleotide polymorphisms (SNPs) that impact disease risk are currently lacking. Here, we use a genetic pleiotropy-informed conditional false discovery rate (FDR) method on GWAS summary statistics data to identify new loci associated with schizophrenia (SCZ) and bipolar disorders (BD), two highly heritable disorders with significant missing heritability. Epidemiological and clinical evidence suggest similar disease characteristics and overlapping genes between SCZ and BD. Here, we computed conditional …