Digital Commons Network^™

The Biomechanics Of The Tendu In Closing To The Traditional Position, Pli#233; And Relev#233;, Nyssa Catherine Masters

USF Tampa Graduate Theses and Dissertations

Dancers spend many years practicing repetitive movements in order for their bodies to gain flexibility, strength and muscle memory. This thesis investigated the biomechanics of a dance student's knee motion during a tendu to the front in first, third, and fifth positions. A dancer will often perform 75 or more tendu closing actions during one technique class - particularly in ballet technique. During a tendu the moving foot moves along the floor but the toes never leave the floor. The tendu is used to strengthen the leg muscles, particularly the quadriceps and gluteal muscles. Flexion/extension, abduction/adduction, and rotation angles of …

Prenatal Stress, Depression, And Herpes Viral Titers, Pao-Chu Hsu

USF Tampa Graduate Theses and Dissertations

Recent studies suggest that some cases of prenatal depression may be associated with reactivation of latent infections of the herpesvirus family. The possible relationships among stress, prenatal depression, and herpes viral reactivation in pregnancy are understudied and the molecular pathways such as the neuroimmune biogenic amine pathway are unidentified. Chronic stress shifts the T helper-1 cell (Th1) cytokine profile to a Th2 profile, which favors virus induced pathogenesis and survival. Pregnancy is also associated with a similar Th2 dominance. In non-pregnant individuals, exposure to psychological or physical stress may be associated with latent herpes viral reactivation and could result in …

Phenotypic Characterization Of The Pancreatic-Derived Factor (Pander) Knockout Mouse On Pure C57bl/6 Background, Shari Moak

USF Tampa Graduate Theses and Dissertations

PANcreatic-DERived Factor (PANDER), or FAM3B, is a 235-amino acid protein strongly expressed within and secreted from the endocrine pancreas. Research surrounding PANDER has revealed a large role for the protein in maintaining glucose homeostasis, as evidenced by several Ad-PANDER overexpressing murine models, our lab's pancreas-specific PANDER transgenic overexpressor, and most recently our mixed genetic C57/129J PANDER knockout (PANKO) mouse. However, PANDER's overall role in glycemic regulation and glucose homeostasis has yet to be studied in a purebred C57BL/6J PANDER knockout model. Here we present the first phenotypic characterization of our global PANDER knockout mouse on a C57BL/6J background (PANKO-C57) where …

Differentiating Between Objectification And Animalization: Associations Between Women, Objects, And Animals, Kasey Lynn Morris

USF Tampa Graduate Theses and Dissertations

While it's clear that the objectification of women is a prominent feature of Western society, it is far less clear what it actually means to be objectified. Philosophers, feminist scholars and psychologist agree that objectification involves a denial of humanity, however, the nature of this dehumanization has yet to be explained. Although existing research provides evidence that objectified women are associated both with objects and animals, no research has examined the conditions under which women are likely to be dehumanized by one form or another. Here, I propose that animalization, characterized by an association with animals, occurs when a woman …

Assessing Appropriate Technology Handwashing Stations In Mali, West Africa, Colleen Claire Naughton

USF Tampa Graduate Theses and Dissertations

Proper hand hygiene is the most effective and efficient method to prevent over 1.3 million deaths annually from diarrheal disease and Acute Respiratory Infections (ARIs). Hand hygiene is also indispensable in achieving the fourth Millennium Development Goal (MDG) to reduce the childhood mortality rate by 2/3rds between 1990 and 2015. Handwashing has been found in a systematic review of studies to reduce diarrhea by 47%#37; and is, thus, capable of preventing a million deaths (Curtis et. al., 2003). Despite this evidence, hand washing rates remain seriously low in the developing world (Scott et al., 2008).

This study developed and implemented …

Science Informational Trade Books: An Exploration Of Text-Based Practices And Interactions In A First-Grade Classroom, Virginia Anne Schreier

USF Tampa Graduate Theses and Dissertations

Although scholars have long advocated the use of informational texts in the primary grades, gaps and inconsistencies in research have produced conflicting reports on how teachers used these texts in the primary curriculum, and how primary students dealt with them during instruction and on their own (e.g., Saul & Dieckman, 2005). Thus, to add to research on informational texts in the primary grades, the purpose of this study was to examine: (a) a first-grade teacher's use of science informational trade books (SITBs) in her classroom, (b) the ways students responded to her instruction, and (c) how students interacted with these …

Weather Patterns Associated With Green Turtle Hypothermic Stunning Events In St. Joseph Bay And Mosquito Lagoon, Florida, Kelsey Roberts

USF Tampa Graduate Theses and Dissertations

January of 2010 brought record-breaking cold temperatures to Florida. Such freeze events can upset vulnerable populations of marine life and other species that rely on stable water temperatures. Sea turtles are one group of species that are particularly susceptible to abrupt drops in water temperature. When water temperatures drop below 10°C, a mass hypothermic stunning, or cold-stunning, event for sea turtles can be expected, with many debilitated turtles washing onshore with a very limited time window to be rehabilitated (Foley et al. 2007). The species of sea turtle that appears to cold-stun with the most frequency is the green turtle, …

Molecular Technologies In The Science And Policy Of Florida Largemouth Bass Micropterus Floridanus Management In Florida, Josh Sakmar

USF Tampa Graduate Theses and Dissertations

Advances in molecular technologies have provided conservation biologist with the opportunity to quantify the genetic structure of a population and, in turn develop management guidelines and policies aimed at preserving the genetic diversity of fish stocks challenged by human activities. This thesis examines the status of genetics as applied to the management of freshwater fisheries by state natural resource agencies with a purpose of understanding the keys to a successful genetics program. An online survey was used to investigate the breadth of molecular marker application to freshwater fisheries management by state natural resource departments. Seven questions were posed to 50 …

Resistance And Tolerance To Trematode Parasites In Larval Anurans, Brittany Sears

USF Tampa Graduate Theses and Dissertations

Nearly every species on the planet has at least one parasite, which, by definition, incurs a

cost in the host. Therefore, organisms must resist parasites - preventing or reducing infections -

or tolerate parasites - reducing the costs of infection - in order to maintain their fitness despite

the presence of parasites. Here, I investigated: 1) whether parasitic, larval trematodes (cercariae)

can detect the least resistant tadpole host species, 2) a hypothetical framework for how host life

history impacts the utilization of inflammation and thus, resistance and tolerance, 3) whether a

common anesthesia technique used in experimental infections immunocompromises tadpoles, …

Molecular And Cellular Mechanisms Of Reelin Signaling In The Adult Hippocampus, Justin Howard Trotter

USF Tampa Graduate Theses and Dissertations

The Reelin signaling pathway is critical for neuronal migration during development and the function of excitatory synapses in the adult forebrain. Despite a growing body of evidence implicating impaired Reelin signaling in the pathogenesis of numerous neuropsychiatric and neurodegenerative disorders, including Schizophrenia and Alzheimer's disease, little is known still regarding the specific molecular and cellular mechanisms whereby Reelin signaling modulates the function of synapses to enable normal learning and memory. In this dissertation, we addressed these knowledge gaps by identifying mechanisms of Reelin proteolysis following synaptic potentiation (Chapter 2) and dissociated the synaptic function of Reelin signaling at excitatory (Chapter …

Use Of Stable Isotope And Trace Metal Signatures To Track The Emigration Of Female Blue Crabs, Callinectes Sapidus, From Tampa Bay, Sky Barrington Williams

USF Tampa Graduate Theses and Dissertations

The blue crab, Callinectes sapidus, supports a successful fishery in the Atlantic Ocean and throughout the Gulf of Mexico, with a total landing of 8,158,788 lb. and a total value of $10,562,128 for the state of Florida during 2012 (FWC 2012 Annual Landings Summary). An accurate and comprehensive understanding of the blue crab's life history and seasonal migration behavior is essential in defining effective management strategies for the fishery. Tag recapture studies and ultrasonic tracking methods for studying blue crab migrations are costly in terms of time and resources. In this study an alternative approach, microchemical natural tagging, was …

When Celebrity Women Tweet: Examining Authenticity, Empowerment, And Responsibility In The Surveillance Of Celebrity Twitter, Megan M. Wood

USF Tampa Graduate Theses and Dissertations

This thesis is a textual analysis of stories in online celebrity news articles about celebrity women and their use of Twitter. It adds to the burgeoning discussion about gendered and racialized bodies online using scholarship from critical feminist, surveillance, and digital media studies. Throughout, my work attends to notions of authenticity and surveillance, examining how what I term a "call to authenticity"--the use of technologies of self-surveillance to verify "authentic" displays of the self--serves to animate contradictory post-feminist paradigms of femininity which function together to discipline and subjugate femininity. I ask: How do post-feminist questions of empowerment and responsibility become …

Innate Immune Responses To Respiratory Syncytial Virus: Age-Associated Changes, Terianne Maiko Wong

USF Tampa Graduate Theses and Dissertations

Respiratory syncytial virus (RSV) infection causes ~64 million cases of respiratory disease and 200,000 deaths annually worldwide, yet there is no broadly effective prophylactic or treatment regimen. RSV can produce acute respiratory illness in patients of all ages but strikes the age extremes, infants and the elderly, with highest frequency presumably due to innate immune deficiencies. A higher morbidity and mortality has been reported for the elderly above 65 years of age, which has been attributed to immune senescence. Efforts to generate an effective vaccine have thus far been unsuccessful.

The innate immune system provides the first line of defense …

Development Of A Novel Method For Deriving Thresholds Of Toxicological Concern (Ttcs) For Vaccine Constituents, Jennifer Jessica White

USF Tampa Graduate Theses and Dissertations

Abstract

Safety assessment relating to the presence of impurities, residual materials and contaminants in vaccines is a focus area of research at the United States Food and Drug Administration (FDA). Sponsors who submit Investigational New Drug (IND) applications for new vaccine products must report the results of safety assessments to the Division of Vaccines and Related Products Applications (DVRPA). Scientifically defining thresholds of toxicological concern (TTCs) as they apply to vaccine constituents will provide a useful aid to the sponsors and public regarding safety assessments of compounds for which there is little or no toxicity data. TTCs are mathematically modeled …

Synthesis Of Amphiphilic Α- And Γ-Aapeptides For Antimicrobial, Self-Assembly, And Mineralization Studies, Mohamad N. Amin

USF Tampa Graduate Theses and Dissertations

Seven novel, amphiphilic AApeptides were prepared. Two cationic, lipo-α-AApeptides, NA-75 and NA-77 were found to possess potent antimicrobial activity against Gram-positive bacteria, with almost no hemolytic activity. In addition to NA-75 and 77, four amphiphilic, γ-AApeptides, NA-133, 135, 137, and 139, and one anionic lipo-α-AApeptide, NA-81, were prepared for molecular self-assembly studies, with several interesting nanostructures observed by TEM. Mineralization of calcium carbonate from gaseous CO2 and Ca2+ in the presence of the 7 AApeptide amphiphiles was also observed by optical microscopy. Several AApeptides were found to be able to influence CaCO3 crystal morphology. Another α-AApeptide, NA-63, was synthesized by …

Attitude Toward Digital And Print-Based Reading: A Survey For Elementary Students, Diedre D. Allen

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to create a valid and reliable survey to measure third through fifth grade students' attitudes toward reading across three mediums: print, e-reader, and Internet. The theoretical framework pulls from self-determination theory and affective models to guide the development of a survey intended for use with intermediate elementary students. The Attitude Toward Reading Survey (ATRS) was developed and field-tested, revised, and field-tested again. Data analysis included confirmatory and exploratory factor analysis, Cronbach's alpha reliability, and cumulative logit modeling. The results indicate the survey is a reliable and valid tool for teachers to use. The ATRS …

Investigation Of Colored Dissolved Organic Matter (Cdom) Optical Properties, Nutrients, And Salinity In Coastal Florida: Springshed To Estuaries, Ana Rosa Arellano

USF Tampa Graduate Theses and Dissertations

Optical parameters measured via absorption spectroscopy and high-resolution fluorescence spectroscopy were used to characterize dissolved organic matter (DOM) in the springshed of Kings Bay, a spring-fed estuary located on Florida's Springs Coast. Over the past 40 years, springs supplying groundwater to Kings Bay have shown an increase in nitrate concentration. The overall goal of this project was to fingerprint wells and spring sites with elevated nitrogen concentrations using CDOM optical properties and establish relationships between nutrient and optical parameters. Samples were obtained from various sites: springs, Kings Bay surface (KBS), wells, coastal waters in and at the mouth of Crystal …

Diet-Induced Ketosis And Calorie Restriction In Mouse Models Of Alzheimer's Pathology, Milene Lara Brownlow

USF Tampa Graduate Theses and Dissertations

Dietary manipulations and their pharmacological outcomes have been increasingly studied in neurodegenerative diseases. However, a systematic comparison among different methods in validated animal models of Alzheimer's disease is made necessary due to several different approaches applied in recent studies. Moreover, despite the large body of evidence on the effects of calorie restriction (CR) and ketogenic diets (KDs) on amyloid pathology, no consistent data is available on the effects of calorie restriction, ketogenic diet or ketone supplements on tau pathology in transgenic models of AD. Moreover, the ketogenic diet used in our studies was custom made with low carbohydrate content and …

Defining Semantic Space And Degree Of Association Using Brainwaves: An Erp Investigation Of Alcohol Expectancies, Ty Brumback

USF Tampa Graduate Theses and Dissertations

The current study investigated the cognitive organization of alcohol expectancies using event-related potentials (ERPs). Building on previous behavioral and ERP paradigms, the goal of the current study was to quantify the relationship among alcohol expectancies using ERP indices of salience, congruence, and cognitive distance. The ERP components being evaluated fit perfectly into the alcohol expectancy theory and research; however, implementing specific paradigms to reliably measure individual differences in alcohol expectancies using ERPs has proven to be more elusive than originally thought. This study utilized established cognitive modeling techniques coupled with ERP responses to linguistic stimuli. In essence, this study provides …

Apathy In Parkinson's Disease: A Behavioral Intervention Study, London Butterfield

USF Tampa Graduate Theses and Dissertations

Apathy, a symptom reflecting motivational and self-initiation impairment, is one of the most common neuropsychiatric symptoms in Parkinson's disease (PD), with an average estimated prevalence of 40-45%. Elevated apathy has been associated with a host of negative associates and consequences, including cognitive impairment, poor daily functioning, poor treatment compliance and illness outcome, reduced quality of life, and increased caregiver burden and distress. While some studies have evaluated pharmacologic approaches to the treatment of apathy, few studies have evaluated non-pharmacologic approaches and we have identified no studies that have evaluated the efficacy of non-pharmacologic treatments of apathy in Parkinson's patients despite …

Application And Characterization Of Self-Assembled Monolayers In Hybrid Electronic Systems, Michael Enoch Celesin

USF Tampa Graduate Theses and Dissertations

In this study, we explore ultra-thin insulators of organic and inorganic composition and their potential role as high-speed rectifiers. Typical applications for these structures include IR sensing, chemical detection, high speed logic circuits, and MEMS enhancements. While there are many elements in the functional group required to create a rectifying antenna (rectenna), the primary thrust of this work is on the rectifier element itself.

To achieve these research goals, a very good understanding of quantum tunneling was required to model the underlying phenomenon of charge conduction. The development of a multi-variable optimization routine for tunneling prediction was required. MATLAB was …

Asbestos Exposure In The Research Laboratory, Ediberto D. Garcia

USF Tampa Graduate Theses and Dissertations

Introduction: Asbestos has been employed in a myriad of industrial applications for more than a century. Of the 181 Mt of asbestos produced worldwide, over 31 Mt was consumed in the U.S. The production and consumption of this mineral eventually was found to cause asbestosis, lung cancer and mesothelioma. While the brunt of the human exposure to asbestos occurred in insulation work, many more workers in other jobs were expose to asbestos fibers. Very little is known about the potential exposure to asbestos in research labs.

Goal: In order to investigate potential research lab work exposures, we simulated lab work …

Immobilization And Characterization Of Physisorbed Antibody Films Using Pneumatic Spray As Deposition Technique, Jhon J. Figueroa

USF Tampa Graduate Theses and Dissertations

The immobilization of antibodies on silica surfaces has been a wide and common practice via cross-linking with the formation of covalent bonds between surface and antibody. The formation of antibody thin films on solid surfaces using pneumatic spray (PS) as the deposition technique and the analysis of the surface morphology of these films were investigated during this study. The pneumatic spray method was compared with the covalent bonding method Avidin-Biotin Bridge (ABB). The intensities and capture efficiency tests showed similar results for both techniques with a lower signal-to-noise ratio (SNR) for the PS deposited films. Specificity tests suggested that the …

Optical Investigations Of Neurohypophysial Excitability And Amyloid Fibril Formation, Joseph Leo Foley

USF Tampa Graduate Theses and Dissertations

This dissertation describes the work done on two distinct projects. In the first part I sought to unravel the mechanisms that underlie the activity-dependent modulation of response in the excitation-secretion coupling of the neurohypophysis. In the second part, I optically monitored and analyzed the secondary structure changes accompanying amyloid fibril formation along multiple pathways, under both denaturing and near-physiological conditions.

Neuronal plasticity plays an important role in regulating various biological systems by modulating release of hormones or neurotransmitters. The changing response to the same stimulus, depending on the context and previous stimulation events, is also the basis of learning and …

An(Other) Rhetoric: Rhetoric, Ethics, And The Rhetorical Tradition, Kathleen Sandell Hardesty

USF Tampa Graduate Theses and Dissertations

Rhetoric as a discipline is still touched by the shadow of ancient Greece. Rhetoric was defined famously by Aristotle as the "available means of persuasion," codified into five canons in classical Rome, and has since been a central part of Western education to train speakers and writers to effectively move their audiences. However, particularly beginning in the mid-20th Century, the discipline's understanding of rhetoric as a means of persuasion (or even manipulation) passed down from our ancient roots began to shift to a sense of rhetoric as matters of ethics and a concern for the other. It begs the question: …

Investigation Of Condition Effects On Batch Fecundity Of The Common Snook, Centropomus Undecimalis, In Tampa Bay, Florida, Catherine Michelle Bruger Hayslip

USF Tampa Graduate Theses and Dissertations

Common snook, Centropomus undecimalis, is a popular game fish species that occurs throughout central and southern Florida, yet many questions remain unanswered regarding their reproductive strategies, including estimates of fecundity with relation to size, age, and condition of the female. These relationships are critical to improving stock assessments and fundamental to estimating spawning potential ratio, the measure by which snook are managed. Spawning snook were collected from a known spawning site, Rattlesnake Key, Tampa Bay, Florida, during three consecutive spawning seasons (2009-2011) and batch fecundity for snook was reported for the first time. Of the 43 females suitable for fecundity …

Metabolism In Corals From Antarctica, The Deep-Sea, And The Shallow Subtropics: Contrasts In Temperature, Depth, And Light, Lara Henry

USF Tampa Graduate Theses and Dissertations

Coral habitats span the range from tropical to polar, extremely shallow to thousands of meters deep. The differences in light and temperature experienced in these varied habitats likely affect the metabolic rates of the corals residing there. The metabolism of three coral species from different habitats have been examined to elucidate the effects of these environmental parameters on metabolism, an under-studied aspect of coral biology. For all three species, measurements of oxygen uptake, ammonium excretion, and activity of the enzymes lactate dehydrogenase (LDH), malate dehydrogenase (MDH), and citrate synthase (CS) were used to characterize their metabolism. Off Florida's Gulf coast, …

World Heritage Status, Governance And Perception In The Pitons Management Area, St.Lucia, Vernice Camilla Hippolyte

USF Tampa Graduate Theses and Dissertations

There are currently 962 geographic sites in the world that have been classified as World Heritage. World Heritage is a unique concept, privy to and defined by UNESCO-- the United Nations, Educational, Scientific and Cultural organization, one of the specialized agencies and autonomous organizations established within the UN-United Nations system. World Heritage is governed by an international treaty called the Convention Concerning the Protection of the World Cultural and Natural Heritage, adopted by UNESCO in 1972 (The `Convention'). The inscription of a World Heritage Site or designation of World Heritage Status is highly coveted and considered in UNESCO parlance to …

Climate, Neo-Spinozism, And The Ecological Worldview, Nancy M. Kettle

USF Tampa Graduate Theses and Dissertations

The global community faces ecological problems with the natural environment and cultural impediments to solving them. Natural systems are constantly changing and so are cultural practices. Humans need to address both: the interaction between those dynamic systems, the natural and cultural, because what happens in one system changes things in the other. The changes to the ecosystems are rapid and sometimes irreversible while dealing with them has been inadequate. Environmental movements, including deep ecology, have been at the forefront of the efforts to engage the public, various groups, politicians, and world governments to address environmental problems on a coordinated large …

Variations Of Ghrelin, Growth Hormone, And Insulin-Like Growth Factor I In The West Indian Manatee (Trichechus Manatus), Rachel Lynn Cimino

UNF Graduate Theses and Dissertations

The metabolic hormones ghrelin, growth hormone, and insulin-like growth factor I are influenced by developmental age, sex, and nutritional status in domestic and free-ranging species. However the role these hormones play has not previously been explored in sub-tropical/ tropical mammals. Furthermore, the seasonality of species with less dynamic environmental cues, such as the West Indian manatee, have not been studied. The West Indian manatee is and endangered species distributed in the southeastern United States and throughout the Caribbean basin, and its nutritional physiology is influenced by environmental factors. Understanding the hormone response to nutritional status in this species and its …