Digital Commons Network^™

Formulation Devlopment Of Mesoporous Silica Nanoparticles As An Injectable Delivery System, Amit S. Wani

Wayne State University Dissertations

Our long term goal is to develop a versatile and robust injectable carrier based on Mesoporous Silica Nanoparticles (MSN) for drug/drug combination therapies. The objective of my dissertation was to optimize surface functionality, particle shape and methods of colloidal stabilization of mesoporous silica by PEG for delivery of drug/drug combinations. This was achieved by pursuing the following three aims:

1. Optimize surface functionality of MSN for high drug loading and controlled release

2. Investigate the effect of particle shape and PEGylation on drug delivery in hypoxic tumor cells

3. Develop MSN capable of delivering drug/drug combinations

To investigate the effect …

Intronic Non-Cg Dna Hydroxymethylation And Alternative Mrna Splicing In Honey Bees, Pablo Cingolani, Xiaoyi Cao, Radhika S. Khetani, Chieh-Chun Chen, Melissa Coon, Alya'a Sammak, Aliccia Bollig-Fischer, Susan Land, Yun Huang, Matthew E. Hudson, Mark D. Garfinkel, Sheng Zhong, Gene E. Robinson, Douglas M. Ruden

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

Previous whole-genome shotgun bisulfite sequencing experiments showed that DNA cytosine methylation in the honey bee (Apis mellifera) is almost exclusively at CG dinucleotides in exons. However, the most commonly used method, bisulfite sequencing, cannot distinguish 5-methylcytosine from 5-hydroxymethylcytosine, an oxidized form of 5-methylcytosine that is catalyzed by the TET family of dioxygenases. Furthermore, some analysis software programs under-represent non-CG DNA methylation and hydryoxymethylation for a variety of reasons. Therefore, we used an unbiased analysis of bisulfite sequencing data combined with molecular and bioinformatics approaches to distinguish 5-methylcytosine from 5-hydroxymethylcytosine. By doing this, we have performed the first whole …

Residential Proximity To Industrial Combustion Facilities And Risk Of Non-Hodgkin Lymphoma: A Case–Control Study, Anjoeka Pronk, John R. Nuckols, Anneclaire J. De Roos, Matthew Airola, Joanne S. Colt, James R. Cerhan, Lindsay Morton, Wendy Cozen, Richard Severson, Aaron Blair, David Cleverly, Mary H. Ward

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

Residence near municipal solid waste incinerators, a major historical source of dioxin emissions, has been associated with increased risk of non-Hodgkin lymphoma (NHL) in European studies. The aim of our study was to evaluate residence near industrial combustion facilities and estimates of dioxin emissions in relation to NHL risk in the United States.

Methods

We conducted a population-based case–control study of NHL (1998–2000) in four National Cancer Institute-Surveillance Epidemiology and End Results centers (Detroit, Iowa, Los Angeles, Seattle). Residential histories 15 years before diagnosis (similar date for controls) were linked to an Environmental Protection Agency database of dioxin-emitting …

Beneath The Printed Pattern: Display And Disguise In Ukiyo-E Bijinga, Anna Moblard Meier

Books, pamphlets, catalogues, and scrapbooks

Text panels and selected images from an exhibition curated by Anna Moblard Meier held in the Bryn Mawr College Class of 1912 Rare Book Room, Canaday Library, from September 25, 2013- December 20, 2013.

Hydrofracking: State Preemption, Local Power, And Cooperative Governance, John R. Nolon, Steven E. Gavin

Case Western Reserve Law Review

No abstract provided.

Meat-Wise Eating Habits, J. Haycock, K. Anderson, Roslynn Brain

Environment and Society Faculty Publications

Whether from a cow, pig, bird, fish, or other domestic or game animal, meat is universally enjoyed as part of the cultural culinary experience. It is also an excellent source of protein, which is important for a healthy body. Many people who eat meat, however, are not aware of the impact their consumption has on the environment. This guide provides a set of quick facts and action tools to make wiser choices concerning meat consumption.

Negotiating Successfully In Asia, Michael Benoliel

Research Collection Lee Kong Chian School Of Business

Cross-cultural negotiations are complex, challenging, and difficult to navigate because much of the Asian culture is unstated, implicit, and internalized in subtle behavioral patterns. It is like an iceberg; more is invisible and less is visible. To understand how the Asian negotiation values and practices are different from those in the West, I describe briefly the Asian cultural roots, highlight the major dimensions that differentiate cultures, explore the factors that influence the Asian negotiation processes and outcomes, and provide a list of practical suggestions for negotiating successful deals with Asian negotiators.

Contributions Of Ocular Surface Components To Matrix-Metalloproteinases (Mmp)-2 And Mmp-9 In Feline Tears Following Corneal Epithelial Wounding, A Petznick, M Madigan, Q Garrett, D Sweeney, M Evans

Medical Papers and Journal Articles

Purpose: This study investigated ocular surface components that contribute to matrix-metalloproteinase (MMP)-2 and MMP-9 found in tears following corneal epithelial wounding.

Methods: Laboratory short-haired cats underwent corneal epithelial debridement in one randomly chosen eye (n = 18). Eyeflush tears were collected at baseline and during various healing stages. Procedural control eyes (identical experimental protocol as wounded eyes except for wounding, n = 5) served as controls for tear analysis. MMP activity was analyzed in tears using gelatin zymography. MMP staining patterns were evaluated in ocular tissues using immunohistochemistry and used to determine MMP expression sites responsible for tear-derived MMPs.

Results: …

Efficacy Of Osmoprotectants On Prevention And Treatment Of Murine Dry Eye, W Chen, X Zhang, J Li, Y Wang, Q Chen, C Hou, Q Garrett

Medical Papers and Journal Articles

Purpose: To evaluate the efficacy of osmoprotectants on prevention and treatment of dry eye in a murine model.

Methods: Dry eye was induced in mice by using an intelligently controlled environmental system (ICES). Osmoprotectants betaine, L-carnitine, erythritol, or vehicle (PBS) were topically administered to eyes four times daily following two schedules: schedule 1 (modeling prevention): dosing started at the beginning of housing in ICES and lasted for 21 or 35 days; schedule 2 (modeling treatment): dosing started after ICES-housed mice developed dry eye (day 21), continuing until day 35. Treatment efficacy was evaluated for corneal fluorescein staining; corneal epithelial apoptosis …

Decrease In Hyperosmotic Stress-Induced Corneal Epithelial Cell Apoptosis By L-Carnitine, N Khandekar, M Willcox, S Shih, P Simmons, J Vehige, Q Garrett

Medical Papers and Journal Articles

Purpose: To characterize the osmoprotective properties of L-carnitine on human corneal epithelial cell volume and apoptosis during hyperosmotic stress.

Methods: Human corneal limbal epithelial (HCLE) cells were exposed to culture medium at 300 mOsm (isotonic) or 500 mOsm (hyperosmotic) with or without L-carnitine (10 mM). Induction of apoptosis was detected by quantifying the proteolytic activity of caspase-8, caspase-9, and caspase-3/7 using caspase activity assays, the expression of tumor necrosis factor (TNF)-α with enzyme-linked immunosorbent assay, and annexin V/propidium iodide staining of HCLE cells evaluated with confocal microscopy and flow cytometry. Cell volume changes in response to hyperosmotic stress were analyzed …

Maternal And Infant Infections Stimulate A Rapid Leukocyte Response In Breastmilk, F Hassiotou, A Hepworth, P Metzger, C Lai, N Trengove, P Hartmann, L Filgueira

Health Sciences Papers and Journal Articles

Breastmilk protects infants against infections; however, specific responses of breastmilk immune factors to different infections of either the mother or the infant are not well understood. Here, we examined the baseline range of breastmilk leukocytes and immunomodulatory biomolecules in healthy mother/infant dyads and how they are influenced by infections of the dyad. Consistent with a greater immunological need in the early postpartum period, colostrum contained considerable numbers of leukocytes (13–70% out of total cells) and high levels of immunoglobulins and lactoferrin. Within the first 1–2 weeks postpartum, leukocyte numbers decreased significantly to a low baseline level in mature breastmilk (0–2%) …

Development Of A Gravid Trap For Collecting Live Malaria Vectors Anopheles Gambiae S.L, Sisay Dugassa, Jenny M. Lindh, Florence Oyieke, Wolfgang R. Mukabana, Steven W. Lindsay, Ulrike Fillinger

All Peer-Reviewed Publications

BACKGROUND: Effective malaria vector control targeting indoor host-seeking mosquitoes has resulted in fewer vectors entering houses in many areas of sub-Saharan Africa, with the proportion of vectors outdoors becoming more important in the transmission of this disease. This study aimed to develop a gravid trap for the outdoor collection of the malaria vector Anopheles gambiae s.l. based on evaluation and modification of commercially available gravid traps. METHODS: Experiments were implemented in an 80 m(2) semi-field system where 200 gravid Anopheles gambiae s.s. were released nightly. The efficacy of the Box, CDC and Frommer updraft gravid traps was compared. The Box …

Food $Ense Website, Heidi Leblanc, D. Christofferson, E. Milligan

Nutrition, Dietetics, and Food Sciences Faculty Publications

No abstract provided.

What?: Visual Interpretations Of The Miscommunication Between The Hearing And Deaf, Virginia Shou

Theses and Dissertations

This thesis visualizes the communication challenges both latent and obvious of my daily life as a hard of hearing individual. By focusing on a variety of experiences and examples I demonstrate the implications of a hard of hearing individual’s life. The prints, objects and videos that I have created for my visual thesis aim to enrich the understanding of a broader public on issues regularly faced by Deaf people. At the heart of my work my goal is to generate mutual empathy between the hearing and the Deaf.

Early Adolescent Nicotine Exposure Has Long-Lasting Effects On Cocaine-Induced Behaviors In Mice, Mai Alajaji

Theses and Dissertations

Nicotine is one of the most commonly used drugs among adolescent populations. Given the fact that adolescence is a unique developmental stage, during which nicotine has long-term effects on future drug-taking behavior, it is essential to understand how early exposure to nicotine during adolescence may affect the abuse liability of other drugs. We hypothesize that repeated exposure to low doses of nicotine in adolescence induce age-specific enhancement of the rewarding effects of several drugs of abuse in the conditioned place preference (CPP) test. Furthermore, we predict that these changes in behavioral responses are mediated by nicotine-induced brain region-specific increases in …

Kinetics And Mechanisms Of Accumulation For Liposomal Ciprofloxacin Into Rat Alveolar Macrophages, Sayeed Mossadeq

Theses and Dissertations

The kinetics and mechanism of accumulation for liposomal ciprofloxacin (Lipo-CPFX) into the rat alveolar macrophage NR8383 cells were studied in vitro, in comparison to unformulated ciprofloxacin (CPFX). Upon incubation with CPFX or Lipo-CPFX, cellular drug accumulation was determined from the cell lysates or efflux was from the extracellular media by fluorescence-HPLC. The accumulation for Lipo-CPFX reached the asymptotic values at ≥ 2 hours, which was a result of uptake and efflux. The uptake appeared to be due to liposomes, mediated via cellular energy-independent mechanism like lipid fusion. In contrast, the efflux appeared to be due to ciprofloxacin, partly cellular energy-dependent, …

Cannabinoid Modulation Of Hiv-1 Tat-Stimulated Adhesion Of Macrophage-Like Cells To The Extracellular Matrix, Johnathan Drevik

Theses and Dissertations

HIV-Associated Neurocognitive Disorders (HANDs) are becoming one of the largest problems in patients infected with HIV-1. The ability of infected cells such as monocytes and microglial cells to act as viral reservoirs causes extreme inflammation in the CNS and has led to several different types of neurocognitive problems. Specifically these HIV-1 infected monocytes are able to secrete inflammatory factors such as the regulatory protein Tat which acts as a chemoattractant for monocytes while also promoting the adhesion of leukocytes to the extracellular matrix (ECM). We have shown that one of the major features of the Tat protein is that it …

Calcium/Calmodulin-Dependent Protein Kinase Ii Beta (Camkiiβ): A Regulator Of Oligodendrocyte Maturation And Myelination, Christopher Waggener

Theses and Dissertations

Oligodendrocytes are cells located in the central nervous system (CNS) that are responsible for the production of the lipid rich membrane, myelin. Myelin and the process of making and wrapping myelin around an axon (also known as myelination) are critical for normal development since they ensure proper signal conduction in the vertebrate CNS. The loss or damage of this myelin, which is typically associated with the demyelinating disease multiple sclerosis (MS), is associated with improper axonal protection along with disrupted nerve signaling which can lead to a variety of different debilitating phenotypic responses. It has been shown that there are …

The Metabolic Transitions Regulated By The Estrogen-Related Receptor (Err) In Drosophila Melanogaster, Yan Li

Theses and Dissertations

In multicellular organism, bioenergetic metabolism is strictly regulated toward efficient generation of ATP. However, in certain situations, such as in limiting oxygen or in the rapidly proliferating system like growing juvenile or cancer cells, organisms apply the metabolic strategy that favors the production of biomass (e.g., nucleotides, amino acids, and lipids) over efficiency of ATP generation. The conserved estrogen-related receptors (ERRs) are master regulators in controlling metabolic homeostasis, and good candidates for mediating the metabolic transition induced by hypoxia and development. First, we investigate how dERR influences hypoxic adaptation in Drosophila melanogaster. We find that dERR is required for a …

In Vitro Lung Epithelial Cell Transport And Anti-Inflammatory Activity For Liposomal Ciprofloxacin, Ruba Darweesh

Theses and Dissertations

Liposomal ciprofloxacin (Lipo-CPFX) is being developed for inhalation, with a goal of sustaining the therapeutic activity, compared to unformulated ciprofloxacin (CPFX). However, the kinetics and mechanism of its sustained local lung retention and pharmacological activity are yet to be fully characterized. This project hypothesized that Lipo-CPFX enables slower and sustained lung epithelial transport and uptake, compared to CPFX, thereby producing prolonged local pharmacological actions. The human bronchial epithelial Calu-3 cells were used as monolayers to characterize the kinetics and mechanism of transport and/or uptake, and to assess the effects of such slow kinetics for Lipo-CPFX on its inhibition against lipopolysaccharide …

White Trash, Heather Law

Theses

White Trash, is body of work that aims to reflect upon our personal role and contribution to the solid waste stream through routine consumption and waste disposal. From everything we eat and drink, to the refuse we flush down the drain, we all consume resources. Living in a materially saturated, mass-consumer society, we have evolved into an increasingly disposable culture. By identifying with our waste production, we then can better evaluate the pitfalls of our materialistic ways. Common consumer products stuffed inside a toilet creates a recognizable waste pattern found within the domestic household. Our trash is considered pollution. Once …

La Carte D’Identité De Jean-Marie Adiaffi Ou La Quête Identitaire À Travers La Symbolique De L’Initiation Allégorique, Theodore Akohoue

The Coastal Review

Since the earliest contact between Sub-Saharan Africans and Europeans, European literature tends to erase African history and civilization. Later, after an unsuccessful erasure, colonial literature denigrates it as primitive. Using the “identity card” as a motif in his novel, Jean-Marie Adiaffi exposes the defects of European colonization in its African colonies. Through an approach of allegorical initiation symbolism, Addiaffi revalorizes his culture, tradition, and identity all denied by colonial rule.

The Evolution Of Animal Identification In Beef Cattle, Kristy E. Boehler

Nebraska Law Review

The United States’ beef cattle industry’s recent past has been plagued by a lack of a national animal identification system. The lack of a national animal identification system has made it difficult, if not impossible, to track a diseased animal back to its farm of origin or determine what other cattle have been in contact with the diseased animal. In fact, during the investigation of the December 2003 Bovine Spongiform Encephalopathy (Mad Cow Disease) outbreak, the lack of a national animal identification system meant that the United States was only able to locate twenty-eight of the eighty cows that entered …

Like A Glass Slipper On A Stepsister: How The One Ring Rules Them All At Trial, Cathren Koehlert-Page

Nebraska Law Review

This article will present the literary concept of endowed objects, provide examples of endowed objects in literature, provide examples of endowed objects in trials, and then discuss how attorneys can identify and use evidence to create an endowed object. Part II of this article explains why endowed objects are useful at trial. Part III defines endowed objects, explains the similarities and differences between endowed objects and other symbols, and illustrates the use of both in fiction and at trial. Part IV describes the process of endowing an object in both fiction narratives and legal narratives. Part V discusses how endowed …

Masthead And Table Of Contents

Nebraska Law Review

No abstract provided.

It Is Time The Law Begins To Protect Consumers From Significantly One-Sided Arbitration Clauses Within Contracts Of Adhesion, Anjanette Raymond

Nebraska Law Review

Businesses are accused of using arbitration as a private dispute resolution system that shields their transgressions from public scrutiny, uses arbitrators and institutions that are perceived as biased, and prevents any real means of justice to the consumer. Something must be done to ensure that consumers facing mandatory arbitration clauses within contracts of adhesion are protected from businesses that seek to use arbitration as an advantageous means to resolve disputes. The American public, a section of Congress, and the arbitration community are all responding, but out-of-date law and recent decisions are thwarting their efforts. It is time for Congress to …

Hydropower: It’S A Small World After All, Gina S. Warren

Nebraska Law Review

Part I of this Article will explore the history and rise of hydroelectric power generation—from water mills to mega dams—and its regulation in the United States. The water mill was one of the first mechanisms used by mankind to harness power. It is a simple use of momentum to create energy. Water mills were used to grind grain, saw logs, create textiles, and fashion tools. As societies evolved, so did the use of water mills, and once electricity and generators arrived on the scene in the late 1800s, hydropower proved to be an efficient means of producing electricity. By the …

Children And Categorization: Maintaining A Standard For Recognizing Speech Categories In Brown V. Entertainment Merchants Ass’N, 131 S. Ct. 2729 (2011), Erick D. Reitz

Nebraska Law Review

The freedom to speak one’s mind free of persecution has long occupied a place of particular gravity in American culture. At its core is the idea that government cannot control our access to certain ideas by restricting some messages but not others—it prohibits government from discriminating against content. To be workable, however, this right must acknowledge exceptions, and it does so by allowing discrimination against certain types of speech to bypass this strict First Amendment protection. So while the Supreme Court prohibits discrimination of most content, there are a few types of speech, such as libel or incitement, that receive …

Forging Toward Coexistence, Laurie J. Beyranevand

Nebraska Law Review

This Article argues that because current federal policies hamper the ability of organic and non-GE farmers to ensure the safety and authenticity of their products, the AC21 needs to meet its charge and reach consensus on USDA supported mechanisms that encourage and facilitate farmers to develop long term, viable solutions that address the interests of all parties. Without USDA support, farmers have little incentive to attempt collaboration under the umbrella of existing federal policies. Part II of this Article provides a detailed history of the regulatory scheme that governs genetically engineered plants and products and demonstrates the clear preference for …

Private Memorials On Public Space: Roadside Crosses At The Intersection Of The Free Speech Clause And The Establishment Clause, Amanda Reid

Nebraska Law Review

Private roadside memorials are part of a growing trend among the bereaved who seek to “make sense of senseless deaths” along the public roadways.2 These memorials, which often feature a Latin cross, are part of deeply meaningful bereavement practices that support the grieving process. As such, these memorials are created to satisfy a human need during a time of crisis and are, therefore, largely constructed without regard to the legality of erecting such markers.3 Courts have yet to provide clear guidance on the constitutionality of erecting and maintaining these private memorials on public spaces. This Article considers the constitutional guideposts …