Digital Commons Network^™

In Vivo Measurement Of Apolipoprotein E From The Brain Interstitial Fluid Using Microdialysis, Jason D. Ulrich, Jack M. Burchett, Jessica L. Restivo, Dorothy R. Schuler, Philip B. Verghese, Thomas E. Mahan, Gary E. Landreth, Joseph M. Castellano, Hong Jiang, John R. Cirrito, David M. Holtzman

Open Access Publications

No abstract provided.

Emerging Roles For Immunomodulatory Functions Of Free Isg15, Jessica A. Campbell, Deborah J. Lenschow

Open Access Publications

No abstract provided.

Diapause And Anhydrobiosis In Embryos Of Artemia Franciscana : Metabolic Depression, Lea Proteins And Water Stress., Steven Handel, Yuvraj Patil, Shumin Li, Nilay Charkraborty, Arpurva Borcar, Michael Menze, Leaf Boswell, Daniel Moore, Mehmet Toner

Faculty and Staff Scholarship

Metabolic depression is typically correlated with extended survival of environmental challenge and energy-limitation in early life stages of various invertebrates and vertebrates. Diapause is an ontogenetically-programmed reduction of development and often metabolism seen in many invertebrates. When embryos of Artemia franciscana enter the state of diapause, the overall metabolic depression is estimated to be greater than 99%. These embryos also contain trehalose and express multiple isoforms of Late Embryogenesis Abundant (LEA) proteins, constituents often present in a number of such anhydrobiotic animals. The mRNA levels for LEA proteins are highest in diapause and post-diapause embryos that possess desiccation tolerance, but …

Grutter's Denouement: Three Templates From The Roberts Court, Ellen D. Katz

Articles

Precedent from the Roberts Court shows the Justices taking three distinct approaches to precedent they dislike. Each provides a template for the Court to criticize race-based affirmative action in higher education, as Fisher v. University of Texas at Austin is widely expected to do. Most narrowly, the Court might use Fisher to issue a warning, much like it did in 2009 when it sidestepped a constitutional challenge to the Voting Rights Act; under this approach, the opinion would spell out why the Justices think the diversity celebrated in Grutter v. Bollinger no longer provides sufficient justification for the use of …

Transcriptome Of The Eastern Oyster Crassostrea Virginica In Response To Bacterial Challenge, Ian Mcdowell

Open Access Master's Theses

The Eastern oyster Crassostrea virginica, an ecologically and economically important estuarine organism, suffers mortalities as high as 90-100% in affected areas due to Roseovarius Oyster Disease (ROD), caused by the bacterial pathogen, Roseovarius crassostreae. Advanced genotypic breeding techniques necessitate information regarding markers and genes associated with disease resistance. As yet, the host-pathogen interaction between C. virginica and R. crassostreae is poorly understood at the molecular level. The identification of potential genes and pathways responsible for an effective host defense response in the Eastern oyster to R. crassostreae is important not only to provide a basis for enhanced breeding techniques, …

Myostatin And Tgf-Β Function In The Regulation Of Myogenesis In Rainbow Trout (Oncorhynchus Mykiss), A Basal Teleost, Ian Morrison Jaffe

Open Access Master's Theses

Myogenesis in vertebrates is a complex and intricately regulated process, various mechanisms of which have been alternately conserved or lost throughout the course of vertebrate evolution. Mammalian myogenesis is highly regulated by myostatin, a member of the Transforming Growth Factor-β (TGF-β) family of cytokines, however, a similar role for myostatin in fish currently remains the subject of inquiry. Myostatin initiates signal transduction by means of the activin type IIb receptor, and shares considerable sequence and structural homology across vertebrate taxa, though its functional homology remains unclear. Two families of transgenic (TG) rainbow trout (RBT), one in which myostatin was inhibited …

Limited Entry In Rhode Island Tracing An Idea From Open Access To License Limitation To Catch Shares, Jonathon M. Peros

Open Access Master's Theses

For centuries fisheries institutions have shaped Rhode Island’s storied fishing tradition, a hallmark of which has been access to marine resources by the residents of the state. In 2001, after a series of moratoriums on commercial fishing licenses, Rhode Island embarked on an extensive public process through the Coastal Institute at the University of Rhode Island to identify fishery management options based on the input of stakeholders. The outcome of that process was the passage of Senate Bill 2771 sub A – otherwise known as Rhode Island Gen. Law 20.2-1 (2002). The legislation ended a moratorium on the issuance of …

Modulation Of Cd8+ T Cell Responses To Aav Vectors With Igg-Derived Mhc Class Ii Epitopes, Daniel J. Hui, Etiena Banser-Tschakarjan, Yifeng Chen, Robert J. Davidson, George Buchlis, Mustafa Yazicioglu, Gary C. Pien, Jonathan D. Finn, Virginia Haurigot, Alex Tai, David W. Scott, Leslie P. Cousens, Shangzhen Zhou, Anne S. De Groot, Federico Mingozzi

Institute for Immunology and Informatics Faculty Publications

Immune responses directed against viral capsid proteins constitute a main safety concern in the use of adeno-associated virus (AAV) as gene transfer vectors in humans. Pharmacological immunosuppression has been proposed as a solution to the problem; however, the approach suffers from several potential limitations. Using MHC class II epitopes initially identified within human IgG, named Tregitopes, we showed that it is possible to modulate CD8⁺ T cell responses to several viral antigens in vitro. We showed that incubation of peripheral blood mononuclear cells with these epitopes triggers proliferation of CD4⁺CD25⁺FoxP3⁺ T cells that …

Making Vaccines "On Demand", Anne S. De Groot, Leo Einck, Leonard Moise, Michael Chambers, John Ballantyne, Robert W. Malone, Matthew Ardito, William Martin

Institute for Immunology and Informatics Faculty Publications

The integrated US Public Health Emergency Medical Countermeasures Enterprise (PHEMCE) has made great strides in strategic preparedness and response capabilities. There have been numerous advances in planning, biothreat countermeasure development, licensure, manufacturing, stockpiling and deployment. Increased biodefense surveillance capability has dramatically improved, while new tools and increased awareness have fostered rapid identification of new potential public health pathogens. Unfortunately, structural delays in vaccine design, development, manufacture, clinical testing and licensure processes remain significant obstacles to an effective national biodefense rapid response capability. This is particularly true for the very real threat of “novel pathogens” such as the avian-origin influenzas H7N9 …

Degradation Of Mangrove Tissues By Arboreal Termites (Nasutitermes Acajutlae) And Their Role In The Mangrove C Cycle (Puerto Rico): Chemical Characterization And Organic Matter Provenance Using Bulk Δ13C, C/N, Alkaline Cuo Oxidation‐Gc/Ms, And Solid‐State 13C Nmr, Christopher H. Vane, Alexander W. Kim, Vicky Moss-Hayes, Collin E. Snape, Miguel Castro Diaz, Nicole S. Khan, Simon E. Engelhart, Benjamin P. Horton

Geosciences Faculty Publications

Arboreal termites are wood decaying organisms that play an important role in the first stages of C cycling in mangrove systems. The chemical composition of Rhizophora mangle, Avicennia germinans, and Laguncularia racemosa leaf, stem, and pneumatophore tissues as well as associated sediments was compared to that of nests of the termite Nasutitermes acajutlae. Nests gave δ¹³C values of −26.1 to −27.2‰ (±0.1) and C/N of 43.3 (±2.0) to 98.6 (±16.2) which were similar to all stem and pneumatophores but distinct from mangrove leaves or sediments. Organic matter processed by termites yielded lignin phenol …

Regulatory T Cell Epitopes (Tregitopes) In Igg Induce Tolerance In Vivo And Lack Immunogenicity Per Se, Yan Su, Robert Rossi, Anne S. De Groot, David W. Scott

Institute for Immunology and Informatics Faculty Publications

Tregitopes are a set of epitopes, derived from IgG, that bind to MHCII, activate nTregs, and promote tolerance. We have now confirmed that coadministration of Tregitopes with a range of proteins (autoantigens and nominal antigens, such as OVA) in vitro and in vivo leads to suppression of T cell and antibody responses to the test antigens. In this study, we demonstrate that Tregitopes are not immunogenic in vivo even when emulsified with strong adjuvants, such as IFA or CFA. Moreover, in vivo administration of Tregitopes with IFA or CFA does not induce Th1 or Th2 cytokine expression under restimulation conditions …

Program Evaluation In The Nonprofit Sector : An Exploratory Study Of Leaders' Perceptions, Gail A. Patin

UNF Graduate Theses and Dissertations

The purpose of the study was to understand the role of and capacity for program evaluation from the perspectives of leaders in the Northeast Florida nonprofit sector. The study was a two-phased qualitative design and had three research questions related to the role of program evaluation, the capacity for program evaluation, and the influence of the relationship between providers and funders on these efforts. The first phase was a nominal group process using the Delphi survey method. The second phase involved in-depth interviews. Study participants were chosen based on specific criteria.

The Delphi phase had four rounds of surveys. Findings …

Communication And Language Recovery In People With Aphasia: Using Gestures In Speech-Language Therapy, Amanda M. Mokaya

Research Papers

No abstract provided.

Animal-Assisted Therapy And The Child-Animal Bond: Children's Well-Being And Behavior, Connie I. Jacobs

Research Papers

No abstract provided.

Examining The Role Of Interagency Coordination When Addressing Flood Risk Management And Flood Disasters In Southern Illinois, Irene J. Upson

Research Papers

No abstract provided.

Turing Test As A Defining Feature Of Ai-Completeness, Roman V. Yampolskiy

Faculty and Staff Scholarship

The paper contributes to the development of the theory of AI-Completeness by formalizing the notion of AI-Complete, C-Complete and AI-Hard problems. The intended goal is to provide a classification of problems in the field of Artificial General Intelligence. We prove Turing Test to be an instance of an AI-Complete problem and further show certain AI problems to be AI-Complete or AI-Hard via polynomial time reductions. Finally, the paper suggests some directions for future work on the theory of AI-Completeness.

Unprotected Sex: The Pregnancy Discrimination Act At 35, Deborah L. Brake, Joanna L. Grossman

Hofstra Law Faculty Scholarship

Thirty-five years ago, Congress passed the Pregnancy Discrimination Act to overturn a Supreme Court decision refusing to recognize pregnancy discrimination as a form of discrimination based on sex. Now, three and a half decades later, women whose work lives are impacted by pregnancy are again finding themselves unprotected from discrimination. Lower court rulings have eviscerated the Act’s protections at the same time that an expansion of worker rights under the Americans with Disabilities Act should redound to the benefit of pregnant women by expanding the pool of comparators who receive accommodations. By following trends in discrimination law generally - equating …

What Was Wrong With The Record?, Ellen D. Katz

Articles

Shelby County v. Holder offers three reasons for why the record Congress amassed to support the 2006 reauthorization of the Voting Rights Act (VRA) was legally insufficient to justify the statute's continued regional application: (1) the problems Congress documented in 2006 were not as severe as those that prompted it to craft the regime in 1965; (2) these problems did not lead Congress to alter the statute's pre-existing coverage formula; and (3) these problems did not exclusively involve voter registration and the casting of ballots.

The Dual Standard Of Review In Contracts Clause Jurisprudence, Brenner M. Fissell

Hofstra Law Faculty Scholarship

The article discusses American constitutional law, the U.S. Supreme Court's ruling in the 1977 case United States Trust Co. of New York v. New Jersey, and a reported dual standard of review in regards to Contracts Clause jurisprudence under the the nation's Constitution as of April 2013. Legislative repudiations of private agreements, government contracting, and public choice theory are addressed, along with trusteeship and police powers. A reasonableness analysis for contracts is mentioned.

Impact Of Nutritional Status On The Somatotropic Axis And Ghrelin In Phocid Seals, Rachael Dailey

UNF Graduate Theses and Dissertations

Metabolic hormones and their axes, including the target tissues and receptors, regulate the tissue specific utilization of nutrients with in the body. The purpose of this research was to understand the hormonal control of complex nutrient partitioning mechanisms involved in young, growing animals. Specifically, this involved the investigation of metabolic hormones and the regulation of growth in two common species of phocids (true seals): harbor seal (Phoca vitulina) and Northern elephant seal (Mirounga angustirostris) pups. This longitudinal study examines young phocids from nutritional nadir through realimentation (realimentation) to investigate how metabolic hormones involved in both food intake and nutrient partitioning …

The Eternal Present: Slow Knowledge And The Renewal Of Time, Douglas E. Christie

Theological Studies Faculty Works

No abstract provided.

‘Jesus Wept’: Mourning As Imitation Of Christ In Bernard’S Sermon Twenty-Six On The Song Of Songs, Anna Harrison

Theological Studies Faculty Works

No abstract provided.

Bioactive Glass Scaffolds For The Regeneration Of Load-Bearing Bone, Xin Liu

Doctoral Dissertations

"Biocompatible scaffolds that replicate the structure and function of bone would be ideal bone substitutes for structural bone loss, provided they have the requisite mechanical properties for reliable long-term loading. In this dissertation, strong porous scaffolds of silicate 13-93 bioactive glass, created with two different microstructures, were evaluated to determine their mechanical properties and their capacity to regenerate bone in a rat calvarial defect model. Scaffolds with an oriented microstructure of columnar pores were prepared by unidirectional freezing of camphene-based suspensions, followed by thermal annealing and sintering. By optimizing the freezing conditions, annealing time, and sintering temperature, constructs (porosity = …

Evaluation Of Single Electron Transfer Reduction Products Of 3-Oxoindoles As Antibacterial Agents, Debasish Bandyopadhyay, Irani Biswas, Subrata Laskar, Bidyut Basak

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Chemistry)

Objective: The objective of the present study is to assess the antibacterial properties of six indole derivatives viz, 3-(Naphth-1-y - methyl) –indole (II), 3-(1-Naphthylcarbonyl – indole (III), 3,3' –diformyl-2,2' –bis indole (IV), 1,4- (3,3' –di indolyl) – 1,4-dioxobutane (V), 3-benzoyl indole (VI) and 1,1 – bis – (3-indolyl)- ethane (VII) synthesized by single electron transfer reduction of 3-formylindole (I) against two Gram +ve and two Gram –ve bacteria which usually cause human infections.

Methods: Synthesis of 3-formyl indole from which the indole derivatives were prepared were further characterized by IR and NMR analysis. The six synthesized compounds were screened for …

Photothermal Desorption Of Toluene From Single Walled Carbon Nanotube And Activated Carbon Sorbents, Evan Lee Floyd

All ETDs from UAB

Millions of workers are occupationally exposed to volatile organic compounds (VOC) annually, requiring exposure assessments through air sampling. Reducing time to knowledge while maintaining adequate sensitivity and affordability is a constant struggle. Sorbent sampling is the preferred technique for VOC exposure assessments. Chemical desorption of sorbents is the most common desorption technique, but sensitivity is limited due to the small injection fraction. Thermal desorption has excellent sensitivity, but provides only one analysis per sample and is cost prohibitive. The goal of this dissertation was to develop a desorption technique that bridges the sensitivity gap between chemical desorption (0.1%) and thermal …

The Development Of An In Vitro Surrogate Assay For Screening Protective Antibodies Against Pneumococcal Surface Protein A (Pspa), Kristopher Ruben Genschmer

All ETDs from UAB

PCV13 is the current pneumococcal protein conjugated vaccine made up of the 13 most clinically relevant capsular polysaccharides. While protective, it is limited to a small portion of the overall serotypes of Streptococcus pneumoniae. The possibility of "serotype replacement" by serotypes not covered by the vaccine and the high cost of producing this conjugated polysaccharide vaccine stress the need for a protein based vaccine, one that is more broad in its protection. Pneumococcal surface protein A (PspA) has been shown to be both immunogenic and protective in mice and is a promising protein vaccine candidate. However, there are no current …

Predictors Of Physical Activity In Child And Adolescent Survivors Of Cancer, Margaux Barnes Gilliam

All ETDs from UAB

Childhood cancer survivors are at increased risk for future health problems. As such, physical activity (PA) has been targeted as a health promotion priority in child and adolescent cancer survivors. Despite research suggesting PA has protective health benefits, a large proportion of survivors do not meet PA recommendations. Development of effective interventions to increase PA in pediatric cancer survivors requires an understanding of the processes that determine survivor PA. Research on the determinants of PA in child and adolescent survivors is sparse, with most research focusing on late adolescent, young adult, and adult survivors of childhood cancer. The following papers …

Experience-Dependent Synaptic Integration Of Adult-Generated Dentate Granule Cells, Jessica Hotard Chancey

All ETDs from UAB

The dentate gyrus (DG) contains neural stem cells that continually generate new neurons throughout life. The contribution of adult neurogenesis to hippocampal function is not well understood, but ablation or silencing of adult-generated neurons in the DG disrupts learning and memory, and neurogenesis is altered in many pathologies associated with impaired cognition including Alzheimer's Disease, epilepsy, and schizophrenia. Neurogenesis in the DG is a stepwise process in which neural stem cells leave the cell cycle and undergo an approximately two month long maturation process to form fully functional dentate granule cells (GCs) that are indistinguishable from those formed during development. …

Targeting Ovarian Cancer Through Epigallocatechin Gallate And Sulforaphane Combinational Treatment, Huaping Chen

All ETDs from UAB

The cellular development of resistance to chemotherapy contributes to the high mortality noted in patients affected by ovarian cancer. Novel compounds that can enhance efficacy of conventional chemotherapy or target both drug-sensitive and -resistant ovarian cancers directly are highly desired. Previous epidemiological studies indicate that consumption of green tea and cruciferous vegetables is inversely associated with occurrence of ovarian cancer. Therefore, revealing the effects and mechanisms of major components of green tea (epigallocatechin gallate, EGCG) and cruciferous vegetables (sulforaphane, SFN) on ovarian cancer cells will provide necessary knowledge for developing potential novel treatments for the disease. Since drug resistance generation …

Cortical Thickness In Amnestic Mild Cognitive Impairment And Its Relationships With Neuropsychological Functioning And Financial Capacity, Jacquelynn Nicosia Copeland

All ETDs from UAB

Diagnostic criteria for amnestic mild cognitive impairment (aMCI) include subjective memory complaint, objective memory impairment, preserved general cognitive function, intact functional abilities, and absence of dementia diagnosis (Petersen, 2004). However, there is evidence to suggest that some aMCI patients show declines in complex instrumental activities of daily living. Under the conceptual model proposed in this study, neuropathological changes occurring in the brain of aMCI and Alzheimer's disease (AD) patients underlie specific forms of neurocognitive dysfunction, which in turn drive decline in everyday functional skills such as financial capacity. The current study examined the relationships between structural brain changes in aMCI, …