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Effects Of Vector Migration On Sylvatic Trypanosoma Cruzi Transmission, Britnee A. Crawford

Mathematics Dissertations - Archive

Vector-borne diseases have had a major impact on global health concerns since their discovery in the 1800's. A vector-borne disease is one transmitted to human or animal host via an invertebrate vector (usually an insect). Chagas' disease, caused by the parasite Trypanosoma cruzi, is transmitted via insect vectors from the Triatoma family. Although human infection with Chagas' is of importance, the disease is maintained in sylvatic (in the wild) transmission cycles. This study examines the effects of vector migration on the spread of T. cruzi in certain sylvatic cycles in the southeastern U.S. and Mexico from several angles. First, a …

Anticancer Activity And Biophysical Reactivity Of Copper Complexes Of 2-(Benzo[D][1,3]Dioxol-5-Ylmethylene)-N-Alkylhydrazinecarbothioamides, Floyd A. Beckford, Jeffrey Thessing, Alyssa Stott, Alvin A. Holder, Oleg G. Poluektov, Liya Li, Navindra P. Seeram

Faculty Publications

A series of copper complexes were synthesized from benzo[d][1,3]dioxole-5-carbaldehyde (piperonal) thiosemicarbazones (RHpTSC where R = H, CH3, C2H5 or C6H5 (Ph)). The complexes show interesting variations in geometry depending on the thiosemicarbazone; a dinuclear complex [Cu(HpTSC)Cl]2, a mononuclear complex [Cu(RHpTSC)2Cl2] (R = CH3 or C2H5) and another mononuclear complex [Cu(PhHpTSC)(PhpTSC)Cl] was generated. The complexes bind in a moderately strong fashion to DNA with binding constants on the order of 10⁴ M^{− 1}. They are also strong binders of human serum albumin with binding constants near 10⁴ M^{− 1}. The complexes show good in vitro …

Report And Reflections From Year One Of The Nsf Gk- 12 Program At Usm, Sherry S. Herron, J. Hanna, K. Wingo

Faculty Publications

No abstract provided.

Extreme Sports: Are They Worth The Risk?, Angela Walin

3690: A Journal of First-Year Student Research Writing

Overview: Broken bones, head trauma, shark attacks, and casualties all while playing the sport you love. Is it worth it? With extreme sports come extreme risks. There are many controversies over these sports as to whether they are ethical or not and why anybody with the right mindset would consider participating in such events. These sports often involve high speed, great heights, a high level of physical exertion, and highly specialized gear or spectacular stunts. Some popular and quickly growing extreme sports in today’s society are snowboarding, speed and freestyle skiing, surfing, sky diving, mountain climbing, and wake boarding. Many …

Science, C. Mackenzie Brown

Religion Faculty Research

No abstract provided.

A Survey Of Top 200 Drugs—Inconsistent Practice Of Drug Strength Expression For Drugs Containing Salt Forms, Daniel Prohotsky, Fang Zhao

Doctoral External Publications

Many ionizable drugs are developed and marketed as salt forms. However, there are no clear US regulatory guidelines on drug strength labeling for salts. The strengths of some drugs are expressed as salts and some as free acids/bases. This study surveyed the top 200 US drugs to assess the common practice in industry. The top 200 drugs prescribed in the United States were included in this survey. The drugs containing active pharmaceutical ingredient (API) salts were selected for analysis. Generic or combination products with redundant API salts were excluded. The package insert of each selected drug was reviewed, and the …

Stress Hormone In White-Throated Sparrows (Zonotrichia Albicollis) Is Not Influenced By The Cleanliness Of A Cotton Bag, Nidun Daniel, Gregory B. Cunningham

Biology Faculty/Staff Publications

Bird banding stations are used throughout North America to trap and band birds, which allow researchers and government agencies to monitor populations, migration routes, health and a variety of other elements of avian physiology and biology. Birds are commonly trapped in mistnets, placed into a cotton bag, and brought to a research station where they are banded, weighed, measured and released. On some days, the number of birds caught may be so high that birds are left to hang in the bags for 10 – 20 minutes while other birds are processed. During this time in the bag the birds …

Internationalization Of An African University In The Post-Colonial Era: A Case Study Of The University Of Nairobi, Iddah A. Otieno

Theses and Dissertations--Educational Policy Studies and Evaluation

This case study uses post-colonial and dependency theoretical lenses to investigate the forces influencing policy, procedures, and participation in international activity in the post-colonial African university environment of Kenya’s first national public university—the University of Nairobi (UoN). The research addresses (1) the approaches and strategies adopted by UoN to engage in international activity; (2) the changes that have taken place over time in international activity engagement at UoN since the attainment of political independence by the Republic of Kenya; and (3) the rationales driving participation in international activity. This investigation included library research, document analysis, multiple campus visits, and 20 …

Use Of Hybrid Diffuse Optical Spectroscopies In Continuous Monitoring Of Blood Flow, Blood Oxygenation, And Oxygen Consumption Rate In Exercising Skeletal Muscle, Katelyn Gurley

Theses and Dissertations--Biomedical Engineering

This study combines noninvasive hybrid diffuse optical spectroscopies [near-infrared spectroscopy (NIRS) and diffuse correlation spectroscopy (DCS)] with occlusive calibration for continuous measurement of absolute blood flow (BF), tissue blood oxygenation (S_tO₂), and oxygen consumption rate (VO₂) in exercising skeletal muscle. Subjects performed rhythmic dynamic handgrip exercise, while an optical probe connected to a hybrid NIRS/DCS flow-oximeter directly monitored oxy-, deoxy-, and total hemoglobin concentrations ([HbO₂], [Hb], and [tHb]), S_tO₂, relative BF (rBF), and relative VO₂ (rVO₂) in the forearm flexor muscles. Absolute baseline BF and …

Identifying Hot-Spots Of Fecal Contamination In The Royal Spring Karstshed, Samuel C. Lee

Theses and Dissertations--Civil Engineering

The City of Georgetown, Kentucky relies on a vast karst spring network as a drinking water source. This karst feature has several inputs from sinkholes and streams in the Cane Run Watershed: a watershed associated with a variety of land uses in the recharge area. The recharge area encompasses the area from North Lexington to Georgetown and is composed of urban, suburban, agricultural and industrial usage. A serious water quality issue exists with respect to the impact of fecal contamination within the spring recharge area. Identification of fecal contamination is quantified by microbial indicators adapted from surface water applications: fecal …

Functionalized Silica Materials And Mixed-Matrix Membranes For Environmental Applications, Noah Daniel Meeks

Theses and Dissertations--Chemical and Materials Engineering

Functionalized silica materials are synthesized for various environmental applications. The overall objective is functionalization with sulfur-containing moieties for mercury sorption and as a platform for nanoparticle synthesis. The first objective is quantifying this functionalization for various silica platforms. The second objective is development of effective mercury sorbents, for both aqueous mercury and elemental mercury vapor. Third, those sorbents are incorporated into mixed matrix membranes (MMM) for aqueous mercury sorption. Fourth, functionalized silica materials are developed as platforms for the synthesis of reactive metal nanoparticles (NP) for the degradation of trichloroethylene.

Thiol-functionalized silica is used as a sorbent for aqueous mercury, …

Inner Bluegrass Agriculture: An Agroecological Perspective, 1850-1880, Andrew Parker Patrick

Theses and Dissertations--History

This study examines agriculture in the Inner Bluegrass Region of Central Kentucky from 1850 to 1880. It utilizes an agroecological perspective, which interprets agriculture through the lens of ecology, to highlight the complex natural and cultural factors that combined to form one of the nation's most prosperous agricultural systems during the nineteenth century. Chapter One explores the agroecosytem Bluegrass farmers created and maintained, emphasizing dynamics in crop and livestock diversity and agricultural technology. Chapter Two examines the African-American labor force that played a key role in shaping the system, first as slaves and later as free men and women. Chapter …

The Life And Work Of Gloria Anzaldúa: An Intellectual Biography, Elizabeth Anne Dahms

Theses and Dissertations--Hispanic Studies

The writings and life of Gloria Evangelina Anzaldúa (1942-2004) have had an immense impact in a variety of disciplines. Her oft-cited text Borderlands/La Frontera: The New Mestiza (1987) is included in many university courses’ reading lists for its contributions to discourses of hybridity, linguistics, intersectionality and women of color feminism, among others. Unfortunately, most scholars content themselves with the intricacies of Borderlands to the neglect of her corpus of work, which includes essays, books, edited volumes, children’s literature and fiction/autohistorias. This analysis presented here wishes to expand our understandings of Anzaldúa’s work by engaging with her pre- and post-Borderlands …

Literary Africa: Spanish Reflections Of Morocco, Western Sahara, And Equatorial Guinea In The Contemporary Novel, 1990-2010, Mahan L. Ellison

Theses and Dissertations--Hispanic Studies

This dissertation analyzes the strategies that Spanish and Hispano-African authors employ when writing about Africa in the contemporary novel (1990-2010). Focusing on the former Spanish colonial territories of Morocco, Western Sahara, and Equatorial Guinea, I analyze the post-colonial literary discourse about these regions. This study examines the new ways of conceptualizing Africa that depart from an Orientalist framework as advanced by the novelists Lorenzo Silva, Concha López Sarasúa, Ramón Mayrata, María Dueñas, Fernando Gamboa, Montserrat Abumalham, Javier Reverte, Alberto Vázquez-Figueroa, and Donato Ndongo. Their works are representative of a recent trend in Spanish letters that signals a literary focus on …

Deconstructing An Icon: Fidel Castro And Revolutionary Masculinity, Krissie Butler

Theses and Dissertations--Hispanic Studies

The goal of this project is to investigate the way in which various representations of Fidel Castro, between the years 1957-1965, have left an indelible mark on Cuba, transforming its landscape, I argue, through gendered means and conscious strategies. Thus it is less concerned with Fidel as an historical person than with examining with a gendered lens the ways in which he has been represented in foundational photographs, interviews, songs, and texts (both narrative and poetry as well as blogs). Drawing from theories of masculinity, which conceive masculinity as both a social construction and material body, my dissertation explores the …

Central And Peripheral Regulation Of Circadian Gastrointestinal Rhythms, Jaclyn Malloy

Theses and Dissertations--Biology

Circadian clocks are responsible for daily rhythms in gastrointestinal function which are vital for normal digestive rhythms and health. The present study examines the roles of the circadian pacemaker, the suprachiasmatic nuclei (SCN), and the sympathetic nervous system in regulation of circadian gastrointestinal rhythms in Mus musculus. Surgical ablation of the SCN abolishes circadian locomotor, feeding, and stool output rhythms when animals are presented with food ad libitum, while restricted feeding reestablishes these rhythms temporarily. In intact mice, chemical sympathectomy with 6- hydroxydopamine has no effect on feeding and locomotor rhythmicity, but attenuates stool output rhythms. Again, restricted …

The Biology And Management Of Brucellosis In Yellowstone Bison, John Joseph Treanor

Theses and Dissertations--Biology

Disease management along the boundaries of wildlife reserves is a growing conservation problem worldwide, as infected wildlife can migrate outside protected areas and pose a threat to livestock and human health. The bison Bison bison population in Yellowstone National Park has long been infected with Brucella abortus, the bacterium causing bovine brucellosis. Concern over migratory bison transmitting B. abortus to cattle herds on lands adjacent to Yellowstone has led to proposals for bison vaccination. Model simulations suggest that vaccination is unlikely to eradicate B. abortus from Yellowstone bison but could be an effective tool for reducing the level of …

The Role Of Social Interactions On The Development And Honesty Of A Signal Of Status, Sanh K. Diep

Theses and Dissertations--Biology

Badges of status are supposed to have insignificant production costs, so use costs are thought to be most important in ensuring signal reliability. Use costs arise from the use of the status signal in social interactions. Social experiences that arise from the use of inappropriate signals in social interactions may drive mechanisms that result in reduced fitness for inappropriate signalers. The role of social control, probing and familiarity in producing use costs was explored. There was no evidence that social control by dominants produced a cost for cheaters and no evidence that social control by subordinates produced a cost for …

Evaluation Of Heat Shock Protein 70a (Hsp70a) In Chlamydomonas Reinhardtii, Sarah Nicole Short

Theses and Dissertations--Biosystems and Agricultural Engineering

Algae are being considered as a possible tool for carbon dioxide mitigation because they uptake carbon dioxide during photosynthesis. Using flue gas from a coal-fired power plant as a carbon source would allow the algae to remove CO₂ from the flue gas before it is emitted into the atmosphere. Because algae do not grow well at the high temperature, low pH conditions presented by flue gas, the traditional approach has been to alter the flue gas to suit the needs of the algae; however, this work aimed to genetically modify the algae Chlamydomonas reinhardtii to grow better at less …

Ultrasonic Concentration Of Microorganisms, Samuel J. Mullins

Theses and Dissertations--Biosystems and Agricultural Engineering

Concentration of microorganisms from a sample volume would increase the limits of detection of samples used for rapid-detection methods. Rapid detection methods are is advantageous for the food industry to rapidly test for bacteria in order release products on a timely basis. Ultrasonic concentration was considered a promising method for manipulation of microorganisms. An ultrasonic chamber consisting of parallel piezoceramic discs with a reticulated polyurethane foam mesh was used to concentrate Saccharomyces cerevisiae yeast and Escherichia coli bacteria. The concentration of yeast was seen to increase by 200% (from 8.0 x 10⁴ cells mL^-1 to 2.4 x 10 …

Assessment Of Conductivity Sensors Performance For Monitoring Mined Land Discharged Waters And An Evaluation Of The Hydrologic Performance Of The Guy Cove Stream Restoration Project, Travis Pritchard Maupin

Theses and Dissertations--Biosystems and Agricultural Engineering

The surface mining method of mountaintop removal has been shown to adversely affect the water quality and hydrologic characteristics of downstream regions. Based on recent scientific literature, the U.S. EPA issued guidance on the specific conductivity (EC25ºC) of waters discharged from mined lands in the Appalachian Coal Belt Region stating that these waters should have an EC25ºC less than 300-500 μS cm-1. Hence, accurately measuring EC25ºC levels of mine discharged waters has significant implications. Furthermore, the development of reclamation techniques that positively impact the hydrological and water quality aspects of valley fill (VF) discharge is needed. To tackle these questions, …

Evaluation Of Transitions For Testing Agricultural Ventilation Fans With The Fan Assessment Numeration System (Fans), Igor Moreira Lopes

Theses and Dissertations--Biosystems and Agricultural Engineering

The Fan Assessment Numeration System (FANS) is an improved air velocity traverse method for measuring in situ fan performance. The FANS has been widely used, but variations of its test procedure are commonly employed to accommodate physical or operational barriers encountered in the field. This laboratory study evaluated the use of transitions to connect a 1.37m FANS unit to two smaller fans (1.22m and 0.91m diameter) and one 1.37m diameter fan. Tests were conducted with the FANS unit positioned on both intake and discharge sides of the fans. Three different transition angles (30^o, 45^o and 60^{o …}

Evaluation Of Separation Method Additives For The Recovery Of Bacteria From Food Matrices, Jennifer Leanne Frederick

Theses and Dissertations--Biosystems and Agricultural Engineering

The microbiological testing of foods is a well-established science. Due to the severity of foodborne pathogen illnesses, the widespread use and implementation of rapid detection methods in food testing labs is increasingly important. The first step for successful testing is sampling. Surfactants have been highly used in food microbiology, but there is not much, if any, published research about the use of fatty alcohols and chemical dispersants as aids in microbial separation. The microbial extraction efficiency of Escherichia coli K12 and Listeria innocua from hot dogs, spinach, and milk was measured using chemical additives (surfactants, fatty alcohols, and a chemical …

Evaluating The Effectiveness Of Weep Berm Systems For Treating Runoff From A Horse Muck Composting Operation, Ross L. Guffey

Theses and Dissertations--Biosystems and Agricultural Engineering

Two contour weep berms systems were designed and implemented to evaluate their performance at mitigating water quantity problems from a horse muck composting operation. The field-scale study focused on the hydrologic response of a standard contour weep berm and a modified contour weep berm. The modified contour weep berm incorporated a woodchip trench upgradient of a typical standard contour weep design. Monitoring occurred from July 2011 through spring 2012. Eight storm events produced measureable runoff for the standard contour weep berm; however, only five storm events produced measurable runoff for the modified contour weep berm. The largest storm event occurred …

Investigating The Role Of Prion Protein Polymorphisms On Prion Pathogenesis, Eri Saijo

Theses and Dissertations--Microbiology, Immunology, and Molecular Genetics

Transmissible spongiform encephalopathies (TSEs), also known as prion diseases, are lethal and infectious neurodegenerative diseases of humans and animals. The misfolding of the normal, or cellular isoform of the prion protein (PrP^C) into the abnormal disease-associated isoform of PrP (PrP^Sc) could change the properties of PrP, consequently, PrP^Sc has lethal infectivity to transmit diseases. The proteinaceous infectious particle consisting mainly of PrP^Sc is called prion. Transmissibility of prions is strongly influenced by multiple factors including PrP polymorphisms, species barriers (PrP sequence specificity) and prion strains (conformational specificity) by unknown mechanisms. Even though the ability …

Role Of The Immune System And Bioactive Lipids In Trafficking Bone Marrow-Derived Stem Cells In Patients With Ischemic Heart Disease, Ahmed Abdel-Latif

Theses and Dissertations--Microbiology, Immunology, and Molecular Genetics

Acute myocardial infarction (AMI) triggers the mobilization of stem/progenitor cells from bone marrow (BMSPCs) into peripheral blood (PB). The underlying mechanisms orchestrating this mobilization and subsequent homing of BMSPCs to the myocardium are poorly understood. While the role of traditional chemokines in the mobilization and homing of hematopoietic stem cell (HSCs) to BM niches is undisputed, their role in directing BMSPCs to the highly proteolytic environment of the ischemic myocardium is debatable and other redundant mechanism may exist. Based on our observation that bioactive lipids, such as sphingosine-1 phosphate (S1P) and ceramide-1 phosphate (C1P), play an important role in regulating …

Regulation Of Hepatic Gene Expression During Liver Development And Disease, Hui Ren

Theses and Dissertations--Microbiology, Immunology, and Molecular Genetics

My first project was to investigate the role of Hepatocyte Nuclear Factor 1 (HNF1) and Nuclear Factor I (NFI) on alpha-fetoprotein (AFP) promoter activity during liver development. AFP is highly expressed in the fetal liver, silenced at birth, and remains at very low levels in the adult liver. A GA substitution located at -119 of the human AFP promoter is associated with hereditary persistence of AFP (HPAFP) expression in the adult liver (Hum Molec Genet, 1993, 2:379). The -120 region harbors overlapping binding sites for HNF1 and NFI. While it has been shown that the GA substitution increases HNF1 binding, …

Signaling Mechanisms Involved In The Generation Of Human Peripheral Itregs, Mary Catherine Reneer

Theses and Dissertations--Microbiology, Immunology, and Molecular Genetics

Maintaining balance in the human immune system is critical for the body’s ability to discriminate between foreign and self-antigens. This balance is achieved, in part, by a subpopulation of T cells known as induced regulatory T cells (iTregs). Dysregulation of this population may contribute to the onset and progression of cancer, chronic inflammation and autoimmune diseases. Therefore, manipulation of iTreg development holds promising therapeutic potential; however, studying this vital population has proven difficult due to low numbers, heterogeneous cell populations, substantial phenotypic differences between mouse and human cells, and the high plasticity seen in iTregs. These current limitations have prevented …

Crosstalk Between Gastrointestinal Epithelial Cells And Resident Microbiota Promotes Immune Homeostasis, Eric William Rogier

Theses and Dissertations--Microbiology, Immunology, and Molecular Genetics

The gastrointestinal tract houses one of the most dense and diverse communities of bacteria on the planet. The mutualistic relationship between the host and commensal microbe permits the microbe an ideal environment to grow and provides the host with increased caloric intake, maturation of the adaptive immune system, and resistance against invading pathogens. To maintain a system in which both parties benefit, the epithelium has evolved numerous strategies to ensure epithelial cells respond to microbes appropriately and that potentially hazardous commensals remain distanced from the soma proper. Breakdown of these propitiating mechanisms elicits unchecked inflammation and can lead to pathology …

The Role Of Intestinal Epithelial Cells And The Regulation Of The Polymeric Immunoglobulin Receptor In Homeostasis And Inflammation, Aubrey Leigh Frantz

Theses and Dissertations--Microbiology, Immunology, and Molecular Genetics

The mammalian intestine harbors an estimated 100 trillion microorganisms, which normally maintain a mutually beneficial relationship with the host. The intestinal epithelium consists of a single layer of intestinal epithelial cells (IECs) that provides a physical barrier as well as innate immune defense, preventing this vast community of microbes from entering host tissues. Secretory immunoglobulin A (SIgA) acts as the first line of antigen-specific immunity at the interface between the gut microbiota and the intestinal epithelium. Polymeric IgA secreted by plasma cells in the intestinal lamina propria is transported across IECs by the polymeric immunoglobulin receptor (pIgR). Defects in epithelial …