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The Role Of Notch Signaling In The Pathogenesis Of Acute Promyelocytic Leukemia, Nicole Grieselhuber

All Theses and Dissertations (ETDs)

The t(15;17) translocation is found in nearly 98% of acute promyelocytic leukemia: APL, FAB subtype M3) cases and results in the fusion of the promyelocytic leukemia: PML) gene with the retinoic acid receptor alpha: RARA) gene. The fusion product, PML-RARA, encodes a functionally altered transcription factor that is the initiating event in APL. To better understand the transcriptional changes associated with APL pathogenesis, we compared the gene expression profiles of APL samples to those of other acute myeloid leukemia FAB subtypes and of enriched normal human promyelocytes. We identified a signature of genes that are specifically dysregulated in APL relative …

The Role Of Autophagy And Il-17 In Bone Resorption, Carl Deselm

All Theses and Dissertations (ETDs)

Osteoclasts are essential for skeletal homeostasis: Teitelbaum, 2000). These macrophage-lineage cells function by generating a polarized microenvironment between themselves and bone wherein skeletal matrix is degraded. This resorptive compartment is isolated from the general extracellular space by an actin ring which encompasses the ruffled border, a convoluted plasma membrane structure formed by its fusion with lysosome-related vesicles containing an electrogenic H+ATPase, a chloride channel, LAMP-1, and cathepsin K. In consequence, resorption of bone reflects secretion of HCl to mobilize mineral, and cathepsin K to degrade the collagen-rich organic matrix, into the resorptive space: Stenbeck, 2002; Zhao et al., 2008). We …

Atm Deficiency -- A Multifaceted Defect In Lymphocyte Development, Beth Helmink

All Theses and Dissertations (ETDs)

The generation of a functional antigen receptor gene in developing lymphocytes requires that the second exon be assembled through a process known as V(D)J recombination, a process that necessarily involves the generation and repair of DNA double-strand breaks made by the Rag endonuclease. Double strand breaks incurred during G1 of the cell cycle activate ATM, a PI3-kinase-like kinase that, in response to genotoxic DNA damage, is known to phosphorylate hundreds of proteins with unique and diverse functions. Notably, deficiencies in ATM lead to ataxia-telangiectasia, a syndrome characterized by lymphopenia, genomic instability and a predisposition to tumors involving antigen receptor loci …

Glycerin Reformation In High Temperature And Pressure Water, Jason W. Picou

Doctoral Dissertations

"The noncatalytic reformation of glycerin in supercritical water was studied in a Haynes 282 tubular reactor. In order to determine which parameters were the most influential, a 2³ experimental matrix was conducted, with temperatures of 500 and 700⁰C, water/glycerin molar ratios of 3:1 and 13:1, and residence times of 30 and 90 seconds, all at a pressure of 24 MPa. It was found that temperature had the largest effect on the two gasification parameters deemed most important, gasification percentage and hydrogen yield. Based on this, the effect of temperature was further investigated by looking at 5⁰C intervals from 500 to …

Mechanosensitive Channels Protect Plastids From Hypoosmotic Stress During Normal Plant Growth, Kira M. Veley, Sarah Marshburn, Cara E. Clure, Elizabeth S. Haswell

Biology Faculty Research

Cellular response to osmotic stress is critical for survival and involves volume control through the regulated transport of osmolytes [1-3]. Organelles may respond similarly to abrupt changes in cytoplasmic osmolarity [4-6]. The plastids of the Arabidopsis thaliana leaf epidermis provide a model system for the study of organellar response to osmotic stress within the context of the cell. An Arabidopsis mutant lacking two plastid-localized homologs of the bacteria mechanosensitive channel MscS (MscS-like [MSL] 2 and 3) exhibits large round epidermal plastids that lack dynamic extensions known as stromules [7]. This phenotype is present under normal growth conditions and does not …

Plastids And Pathogens: Mechanosensitive Channels And Survival In A Hypoosmotic World, Kira M. Veley, Elizabeth S. Haswell

Biology Faculty Research

In bacteria, MscS-type mechanosensitive channels serve to protect cells from lysis as they swell during extreme osmotic stress. We recently showed that two MscS homologs from Arabidopsis thaliana serve a similar purpose in the epidermal plastids of the leaf, indicating that the plant cell cytoplasm can present a dynamic osmotic challenge to the plastid. MscS homologs are predicted to be targeted to both plastids and mitochondrial envelopes and have been found in the genomes of intracellular pathogens. Here we discuss the implications of these observations, and propose that MS channels provide an essential mechanism for osmotic adaptation to both intracellular …

Organoclay Dispersion In Linear Low-Density Polyethylene And Maleated Linear Low-Density Polyethylene Via Supercritical Carbon Dioxide Processing, Matthew J. Factor

Doctoral Dissertations

"Research into polymer-clay nanocomposites (PCN's) has been ongoing for decades as a result of the property enhancements offered by clay. To fully exploit these property enhancements, organically modified clays (organoclays) are utilized to promote clay delamination by reducing the disparity between the hydrophilicity of the clay and the hydrophobicity of the highly used polyolefin polymer. Since the organic modification of organoclays can degrade at temperatures typical to many polymers during melt-mix processing, this work utilizes the low-temperature processing fluid supercritical carbon dioxide (scCO₂) to disperse an organoclay into the highly used polymer LLDPE and ascertains the associated processing conditions for …

Data Fusion By Using Machine Learning And Computational Intelligence Techniques For Medical Image Analysis And Classification, Beibei Cheng

Doctoral Dissertations

"Data fusion is the process of integrating information from multiple sources to produce specific, comprehensive, unified data about an entity. Data fusion is categorized as low level, feature level and decision level. This research is focused on both investigating and developing feature- and decision-level data fusion for automated image analysis and classification. The common procedure for solving these problems can be described as: 1) process image for region of interest' detection, 2) extract features from the region of interest and 3) create learning model based on the feature data. Image processing techniques were performed using edge detection, a histogram threshold …

Complex Scaling Behavior In Animal Foraging Patterns, Prabhavi Kaushalya Premachandra

Doctoral Dissertations

This dissertation attempts to answer questions from two different areas of biology, ecology and neuroscience, using physics-based techniques. In Section 2, suitability of three competing random walk models is tested to describe the emergent movement patterns of two species of primates. The truncated power law (power law with exponential cut off) is the most suitable random walk model that characterizes the emergent movement patterns of these primates. In Section 3, an agent-based model is used to simulate search behavior in different environments (landscapes) to investigate the impact of the resource landscape on the optimal foraging movement patterns of deterministic foragers. …

Palynomorph And Palynofacies Assemblages Of Neutral-Alkaline And Acid Lakes South Of Norseman, Southern Western Australia, Lutfia Grabel

Masters Theses

"The Yilgarn Craton in Western Australia hosts hundreds of shallow ephemeral hypersaline lakes, the majority of which have acid to neutral pH values. As part of a multidisciplinary study of the evolution of hypersalinity and acidity in the region, three drill cores were studied for their palynofacies and palynomorph contents in order to characterize palynofloral response to environmental changes. Drill cores from two acid lakes, Prado Lake (PL1-09 and PL2-09) and Twin Lake West (TLW1-09), and the neutral-alkaline Gastropod Lake (GLE1-09) south of Norseman recovered Miocene to Holocene sediments of the Revenge and Polar Bear formations. Transmitted light microscopy was …

Depth Profiling Of Pahs Treated With Activated Carbon Using In-Situ Spme, Ryan D. Stringer

Masters Theses

"Novel and efficient methods to measure the bioavailability of hydrophobic organic contaminants (HOCs) in contaminated sediments will play an important role in the acceptance of alternative sediment remediation strategies. In this project, solid phase microextraction (SPME) fibers, protected in perforated steel tubes, were used as in situ passive samplers to measure the treatment of activated carbon (AC) in polycyclic aromatic hydrocarbon (PAH) contaminated sediment. Contaminated sediment was treated with two modes of AC waterjet amendment. In the first treatment, a single 2-min injection was shot into the center of a test vessel and in the second treatment, multiple 7-sec injections …

Meso-Scale Fluidic Devices With Chemical Sensors For Biological Applications, Satya Gowthami Achanta

Doctoral Dissertations

"Molecular oxygen and humidity are some of the major environmental quantities being measured for various industrial and commercial applications. This dissertation focuses on the design, fabrication and characterization of optofluidic biosensor systems for oxygen and humidity quantification using color charge-coupled device (CCD) camera as a photodetector and LED panel as an excitation source. Meso-scale fluidic devices with integrated oxygen and humidity sensors for potential applications to hydrotropic and oxytropic studies of small plant roots have been investigated in this study. Meso-scale sensor platform was fabricated using porphyrine complex as the sensitive dye embedded within Ethyl Cellulose (EC) polymer matrix. Green …

Quantitative Analysis Of Co-Enzyme A In A Plant Tissues And Cancer Biomarkers In Urine Samples By Using Capillary Electrophoresis And Liquid Chromatography-Mass Spectrometry, Yongqing Jiang

Doctoral Dissertations

"Despite continuous effort and progress in cancer detection and therapy, cancer remains one of our greatest health concerns because of its low survival rate and rapid incidence increase. One reason for this is the late detection and therefore pre-cancer diagnosis is crucial. In pre-cancer studies, cancer biomarkers become significant because of the useful information it contains such as possible cancer type and stage. Separation and detection technique plays an important role in cancer biomarker identification.

Coenzyme A (CoA) facilitates more than 100 chemical reactions in cells. Because of its low abundance accumulated in cells, it is critical to develop a …

Fabrication Of Complex Ceramic Parts With Sacrificial Material Using Freeze-Form Extrusion Fabrication, Diego Armando Garcia MontañO

Masters Theses

"An additive manufacturing process called Freeze-form Extrusion Fabrication (FEF) was developed in this study to fabricate complex ceramic parts that require the use of sacrificial materials. The aqueous paste of alumina (Al₂O₃) was used as the main material, with solids loadings as high as 50 vol. % and water as the main liquid medium. Methyl cellulose, with a 10 vol. % solids loading and 90 vol. % water content, was used as the sacrificial material. The freeze-form extrusion machine has multiple extruders (extrusion devices) capable of fabricating parts from multiple materials without mixing them. The dynamic process of extruding alumina …

Using Lidar As A Monitoring Device To Calculate Volume Of Rockfall Over Time, Travis James Kassebaum

Masters Theses

"There are many natural hazards which threaten the stability of highway infrastructure and the safety of motoring public. Rockfall and unstable slopes are one of the major concerns. This thesis describes a method which safely and accurately evaluates rockfall on rock outcrops through the use of Light Detection and Ranging (LiDAR) data. LiDAR is an imaging technology which can be used to measure and evaluate possible rock outcrop hazards such as: rockfall, rock-avalanches, debris flows, and landslides. LiDAR produces a three dimensional point cloud of millions of points which gives spatial data on the scanned object, accurate to millimeters, in …

Investigation Of Biomarkers For Noninvasive Early Diagnosis Of Cancer, Sanjeewa Bandara Gamagedara

Doctoral Dissertations

"Cancer is the second leading cause of death worldwide, and deaths from cancer worldwide are projected to continue rising, with an estimated 12 million deaths in 2030. Cancer occurs when cells in a part of the body begin to grow out of control. It develops very rapidly, and early diagnosis offers a greater chance of successful treatment and a higher survival rate. Biomarkers are good potential tools for early diagnosis. The main hypothesis of this study is that the levels of certain metabolites present in human urine can indicate the current or future behavior of cancer. Therefore, these studies focused …

"...Bless Her Little Heart!": The Culture Of Honor And Emotion Recognition, Forrest J. Rackham

College of Graduate Studies: Theses & Dissertations

Author's abstract: Some researchers assert that cultural display rules may explain differences in perceiving emotions (Matsumoto, Yoo, & Chung, 2010). The current study examined the display rule of masking within the Southern culture of honor. It was hypothesized that masking within the culture of honor negatively affects emotion perception sensitivity, particularly in the speed and accuracy of recognizing anger. Southern undergraduate students were primed with the culture of honor and then presented with the Emotional Expression Multimorph Task. Participants chose one of the six emotions (i.e., sad, happy, surprise, fear, disgust, or anger). It was hypothesized participants in the masking …

Effect Of Honey On Anaerobic Performance In Male Collegiate Soccer Players, Katelyn G. Woolfolk

College of Graduate Studies: Theses & Dissertations

Author's abstract: Carbohydrate supplementation has been associated with increased performance in aerobic and anaerobic activity. Honey, specifically, has shown to increase performance in aerobic exercise as well as provide other health benefits. The purpose of this study was to see if honey, a natural substance, has a positive effect on anaerobic performance, rates of perceived exertion, and gastrointestinal distress. Subjects consisted of 18 healthy, Division 1 collegiate male soccer players (19.39 ± 0.85 yr, 75.39 ± 7.97 kg). All had no allergies and were cleared for participation by a certified athletic trainer. Subjects were randomly assigned to one of three …

Simultaneous Detection Of Six Diarrhea-Causing Bacterial Pathogens With An In-House Pcr-Luminex Assay, Jie Liu, Jean Gratz, Athanasia Maro, Happy Kumburu, Gibson Kibiki, Mami Taniuchi, Arif Mahmud Howlader, Shihab U. Sobuz, Rashidul Haque, Kaisar A. Talukder, Shahida Qureshi, Anita K. M. Zaidi, Doris M. Haverstick, Eric R. Houpta

Department of Paediatrics and Child Health

Diarrhea can be caused by a range of pathogens, including several bacteria. Conventional diagnostic methods, such as culture, biochemical tests, and enzyme-linked immunosorbent assay (ELISA), are laborious. We developed a 7-plex PCR-Luminex assay to simultaneously screen for several of the major diarrhea-causing bacteria directly in fecal specimens, including pathogenic Aeromonas, Campylobacter jejuni, Campylobacter coli, Salmonella, Shigella, enteroinvasive Escherichia coli (EIEC), Vibrio, and Yersinia. We included an extrinsic control to verify extraction and amplification. The assay was first validated with reference strains or isolates and exhibited a limit of detection of 10(3) to 10(5) CFU/g of stool for each pathogen as …

Examination Of Mediational Models Of The Hostility-Health Link, Carol Goulet

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study investigated health behavior as a possible mediator of the link between hostility and poor physical and psychological health. The relations of health behavior, character strength, and virtuous behavior to hostility and health were also examined. A sample of 689 undergraduate participants (69.4% female) completed a series of on-line assessments that measured hostility, physical health, psychological health, health behavior, social support, character strength, and virtuous behavior. Health behavior was examined and found to be a significant partial mediator of the relations between hostility and physical and psychological health. Furthermore, the results of multiple regressions revealed that gender, hostility, and …

Evaluation Of Novel Sigma Receptor Antagonists Against Meth-Induced Neurotoxicity: In Vivo And In Vitro Studies, Nidhi Kaushal

Graduate Theses, Dissertations, and Problem Reports (ETD)

Methamphetamine (METH) is a highly addictive psychostimulant drug of abuse, causing hyperthermia and neurotoxicity at high doses. Currently, there is no clinically proven pharmacotherapy to treat these effects of METH, necessitating identification of potential novel therapeutic targets. Earlier studies showed that METH binds to sigma receptors in the brain at physiologically relevant concentrations, where it acts in part as an agonist. AC927 (1-(2-phenethyl)piperidine oxalate), CM156 (3-(4-(4-cyclohexylpiperazin-1-yl)butyl)benzo[d]thiazole-2(3H)-thione) and SN79 (6-acetyl-3-(4-(4-(4-florophenyl)piperazin-1-yl)butyl)benzo[d]oxazol-2(3H)-one) were synthesized as putative sigma receptor antagonists with nanomolar affinity and selectivity for sigma receptors over 29-80 other binding sites. AC927, CM156 and SN79 pretreatment afforded protection against METH-induced hyperthermia and …

The Role Of Src-Homology 2 Domain-Containing Tyrosine Phosphatase 2 In Signaling And Tumorigenesis Of Basal Type/Triple Negative Breast Cancer, Fatimah Kh. Matalkah

Graduate Theses, Dissertations, and Problem Reports (ETD)

Basal type/triple negative breast cancer (BTBC) represents a distinct tumor group that is characterized by an aggressive disease phenotype and poor clinical outcome. Patients with this subtype cannot benefit from the available anti-hormone and anti-HER2 targeted therapies (e.g. tamoxifen and herceptin). Therefore, characterizing potential therapeutic targets is urgently needed. We reasoned that the Src-homology 2 domain-containing tyrosine phosphatase 2 (SHP2) could serve as a therapeutic target in BTBC, because of its critical regulation of epidermal growth factor receptor (EGFR) which is overexpressed in BTBC. Here, we demonstrated that SHP2 knockdown in BTBC cell lines suppressed cell proliferation, migration, and transformation. …

Treatment Of Nightmares Using Imagery Rehearsal Therapy: A Multiple Baseline Study, Michael R. Nadorff

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nightmares have been linked with several negative consequences, including death by suicide. Further, nightmare sufferers report that nightmares have a significant negative impact on their lives. To address this problem, several nightmare treatments have been studied, with Imagery Rehearsal Therapy (IRT) having the greatest amount of empirical support. However, there are many unanswered questions in regard to IRT, such as how quickly one may see an effect and whether or not there is a temporary increase in nightmares prior to a decrease. The current study aimed to address these questions by examining IRT using a non-concurrent multiple baseline design. Four …

Examining The Dynamics Of Policy Change And U.S. Narcotics Policy: Implications For The Global Narcotics Regime, Felix Kumah-Abiwu

Graduate Theses, Dissertations, and Problem Reports (ETD)

The prohibition approach to narcotics control has been the dominant policy paradigm of the United States (US) and the global narcotics regime under the United Nations (UN) for more than a century. In recent years, however, key questions have been raised on the existing prohibition policy approach, which many consider as ineffective and unchanging from a broader perspective. It is based on this appearance of unchanging policy that this study seeks to investigate whether the US narcotics policy is undergoing any type of change or not. The study also seeks to explore the implications of US narcotics policy change on …

A University Course's Influence On Students' Perpectives Regarding Sabbathkeeping As Spiritual Discipline, Ifeoma I. Kwesi

Doctor of Ministry Projects

Problem

Although most students who attend Oakwood University are self-identified Seventh-day Adventist Christians, from general observation of their activities, conversations, and attitudes displayed on any given Friday night and/or Saturday (i.e., Sabbath), it would be easy to conclude that many neither enter nor exit their collegiate experience with a holistic understanding or experience of Sabbath and Sabbathkeeping.

Methodology

1. As part of the research for this project I used a pre- and post-test methodology to determine the cognitive, behavioral and affective realms of Sabbath and Sabbathkeeping among students enrolled in RP131: Spiritual Formation for Ministry. The intervention was the aforementioned …

Apparent Prevalence Of Swine Brucellosis In Feral Swine In The United States, Kerri Pedersen, Sarah N. Bevins, Brandon S. Schmit, Mark W. Lutman, Michael P. Milleson, Clint T. Turnage, Troy T. Bigelow, Thomas J. Deliberto

Human–Wildlife Interactions

Samples were collected in 35 states as part of a national monitoring system to detect multiple diseases in feral swine (Sus scrofa). During March 2009 through December 2010, we collected serum samples from 4,479 feral swine from 13 states, and 159 animals tested were seropositive for brucellosis. No difference in likelihood of infection was found between males and females, but adults were more likely than sub-adults or juveniles to be exposed to brucellosis. Feral swine sampled during winter months also were more likely to be seropositive than animals sampled during other seasons. Apparent prevalence varied among states, and …

Black Men's Responsible Fatherhood Narratives: Fatherhood, Responsibility, Race, And Gender, Shane Chaplin

Electronic Theses and Dissertations

Over the last few decades increasing rates of single mother households in the United States have triggered a national alarm over the effects of father absence on society. Father absence has been linked specifically to many of the problems plaguing black communities in the United States (e.g. poverty, low educational attainment, etc.) and as a result community and political leaders alike have consistently promoted responsible fatherhood practices as a way to address them. Although responsible fatherhood has received, in this context, a considerable amount of social attention, this attention has come intertwined with considerable political and moral rhetoric at all …

Computational Ligand-Based Cns Therapeutic Design: The Search For Novel-Scaffold Norepinephrine Transporter Inhibitors, Anna Chaly

Electronic Theses and Dissertations

Monoamine transporter (MAT) proteins are responsible for regulating cellular signal transduction through control of neurotransmitter reuptake in the synapse, and are therefore relevant to diseases including addiction, psychosis, anxiety and depression. MATs, specifically the serotonin transporter (SERT or 5-HTT), norepinephrine transporter (NET), and dopamine transporter (DAT), serve as the principal targets for antidepressant drugs, such as SSRIs (selective serotonin reuptake inhibitors), NRIs (norepinephrine reuptake inhibitors) and TCAs (tricyclic antidepressants), as well as psychostimulant drugs of abuse such as cocaine and the amphetamines. Due to a lack of crystallographic MAT data, it is unclear as to which of two MAT protein …

The Lived Experience Of Parenting A Child Adopted From An Institution In Eastern Europe, Kimberly A Curry-Lourenco

Electronic Theses and Dissertations

This study sought to explore the lived experience of parenting a child adopted from an institution in Eastern Europe. A hermeneutic, phenomenologic method following the Utrecht School was used. The setting for this study was a southern state on the east coast of the United States. The sample consisted of 11 participants, nine mothers and two fathers, self- identified as adoptive parents to children from institutions in Eastern Europe. Semi-structured interviews, with thematic analysis of transcriptions were conducted. Five major themes were identified: choosing-being chosen, weaving the fabric, the importance of doing, remaining mindful, and looking-in, looking out. Conclusions were …

Enrichment, Characterization And Identification Of Microbial Communities Associated With Unconventional Shale Gas Production Water, J. Lucas Eastham

Electronic Theses and Dissertations

Unconventional natural gas extraction from the Marcellus Shale requires millions of gallons of water to fracture shale and release natural gas from the formation. This process produces water with high levels of total dissolved solids (TDS); and, efforts to recycle these fluids has stimulated microbial growth in produced water. The objective of this study was to analyze the ionic composition of and characterize microorganisms from Marcellus produced water samples. A semi-synthetic culture medium was designed with high TDS to enrich for halophilic microbes, which yielded robust cultures that were able to grow over a wide range of salinities. DNA extracted …