Digital Commons Network^™

Integrin-Mediated Mechanotransduction Pathway Of Low-Intensity Continuous Ultrasound In Human Chondrocytes, Nicholas P. Whitney, Allyson C. Lamb, Tobias M. Louw, Anuradha Subramanian

Department of Chemical and Biomolecular Engineering: Faculty Publications

Chondrocytes are mechanosensitive cells that require mechanical stimulation for proper growth and function in in vitro culture systems. Ultrasound (US) has emerged as a technique to deliver mechanical stress; however, the intracellular signaling components of the mechanotransduction pathways that transmit the extracellular mechanical stimulus to gene regulatory mechanisms are not fully defined. We evaluated a possible integrin/mitogen-activated protein kinase (MAPK) mechanotransduction pathway using Western blotting with antibodies targeting specific phosphorylation sites on intracellular signaling proteins. US stimulation of chondrocytes induced phosphorylation of focal adhesion kinase (FAK), Src, p130 Crk-associated substrate (p130Cas), CrkII, and extracellular-regulated kinase (Erk). Furthermore, pre-incubation with inhibitors …

Reconstruction And Comparison Of The Metabolic Potential Of Cyanobacteria Cyanothece Sp. Atcc 51142 And Synechocystis Sp. Pcc 6803, Rajib Saha, Alex T. Verseput, Bertram M. Berla, Thomas J. Mueller, Himadri B. Pakrasi, Costas D. Maranas

Department of Chemical and Biomolecular Engineering: Faculty Publications

Cyanobacteria are an important group of photoautotrophic organisms that can synthesize valuable bio-products by harnessing solar energy. They are endowed with high photosynthetic efficiencies and diverse metabolic capabilities that confer the ability to convert solar energy into a variety of biofuels and their precursors. However, less well studied are the similarities and differences in metabolism of different species of cyanobacteria as they pertain to their suitability as microbial production chassis. Here we assemble, update and compare genome-scale models (iCyt773 and iSyn731) for two phylogenetically related cyanobacterial species, namely Cyanothece sp. ATCC 51142 and Synechocystis sp. PCC 6803. …

Crystalline Nano Structures, Barry Chin Li Cheung, Joseph Reese Brewer, Nirmalendu Deo

Department of Chemistry: Faculty Publications

The present invention comprises nano obelisks and nanostructures and methods and processes for same. The nano obelisks of the present invention are advantageous structures for use as electron source emitters. For example, the ultra sharp obelisks can be used as an emitter source to generate highly coherent and high energy electrons with high current.

The Law And Economics Of Fluctuating Criminal Tendencies And Incapacitation, Murat C. Mungan

Faculty Scholarship

Economic analyses of criminal law are frequently and heavily criticized for being unable to explain many criminal law rules and doctrines that people find intuitively just. Existing economic models cannot properly explain, for instance, why criminal law distinguishes between (i) repeat offenders and first-time offenders, (ii) murder and voluntary manslaughter, and (iii) remorseful and non-remorseful offenders.

In this Article, I propose a new and richer economic theory of crime that captures the rationales behind these practices, and potentially behind many other important criminal law principles and doctrines. Unlike an overwhelming majority of previous economic analyses, my theory accounts not only …

A Chemical Proteomic Probe For Detecting Dehydrogenases: Catechol Rhodanine, Xia Ge, Daniel S. Sem

Chemistry Faculty Research and Publications

The inherent complexity of the proteome often demands that it be studied as manageable subsets, termed subproteomes. A subproteome can be defined in a number of ways, although a pragmatic approach is to define it based on common features in an active site that lead to binding of a common small molecule ligand (ex. a cofactor or a cross-reactive drug lead). The subproteome, so defined, can be purified using that common ligand tethered to a resin, with affinity chromatography. Affinity purification of a subproteome is described in the next chapter. That subproteome can then be analyzed using a common ligand …

Charge Delocalization In Self-Assembled Mixed-Valence Aromatic Cation Radicals, Tushar Navale, Khushabu Thakur, Vijay Vyas, Shriya Wadumethrige, Ruchi Shukla, Sergey V. Lindeman, Rajendra Rathore

Chemistry Faculty Research and Publications

The spontaneous assembly of aromatic cation radicals (D^+•) with their neutral counterpart (D) affords dimer cation radicals (D₂^+•). The intermolecular dimeric cation radicals are readily characterized by the appearance of an intervalence charge-resonance transition in the NIR region of their electronic spectra and by ESR spectroscopy. The X-ray crystal structure analysis and DFT calculations of a representative dimer cation radical (i.e., the octamethylbiphenylene dimer cation radical) have established that a hole (or single positive charge) is completely delocalized over both aromatic moieties. The energetics and the geometrical considerations for the formation …

A Seminaphthofluorescein-Based Fluorescent Chemodosimeter For The Highly Selective Detection Of Cysteine, Xiaofeng Yang, Yixing Guo, Robert M. Strongin

Chemistry Faculty Publications and Presentations

A fluorescent chemodosimeter for cysteine detection was developed based on a tandem conjugate addition and intramolecular cyclization reaction. The method exhibited an excellent selectivity for cysteine over other biothiols such as homocysteine and glutathione.

Overview Of The 2010 Carbonaceous Aerosols And Radiative Effects Study (Cares), Rahul A. Zaveri, William J. Shaw, Daniel J. Cziczo, Beat Schmid, Richard Ferrare, M. Lizabeth Alexander, Raul Alvarez, W. Patrick Arnott, Dean B. Atkinson, Sunil Baidar, Robert M. Banta, James Barnard, Josef Beranek, Larry K. Berg, Fred Brechtel, W. Alan Brewer, John F. Cahill, Brian Cairns, Christopher D. Cappa, D. Chand, S. China, Jennifer M. Comstock, Manvendra K. Dubey, Robert C. Easter, M. H. Erickson, Jerome Fast, Cody Floerchinger, B. Flowers, Edward C. Fortner, Jeffrey S. Gaffney, Mary K. Gilles, Kyle Gorkowski, William Gustafson, Madhu Gyawali, J. Hair, R. M. Hardesty, Joseph Harworth, Scott C. Herndon, Naruki Hiranuma, Chris Hostetler, John M. Hubbe, John T. Jayne, H. Jeong, B. Tom Jobson, Evgueni Kassianov, Lawrence I. Kleinman, C. Kluzek, W. Berk Knighton, Katheryn R. Kolesar, Chongai Kuang, Alena Kubátová, Andrew O. Langford, Alexander Laskin, N. Laulainen, Richard D. Marchbanks, Claudio Mazzoleni, Fan Mei, Ryan C. Moffet, Dan Nelson, Michael Obland, Hilke Oetjen, Timothy B. Onasch, I. Ortega, Matteo Ottaviani, Mikhail Pekour, Kimberly A. Prather, James Gregory Radney, Raymond R. Rogers, Scott P. Sandberg, Arthur J. Sedlacek, Christoph J. Senff, Gunnar I. Senum, Ari Setyan, John E. Shilling, Manishkumar Shrivastava, C. Song, Stephen R. Springston, R. Subramanian, Kaitlyn Suski, Jason Tomlinson, Rainer M. Volkamer, H. W. Wallace, Jian Wang, A. M. Weickmann, Douglas R. Worsnop, Xiao-Ying Yu, Alla Zelenyuk, Qi Zhang

Chemistry Faculty Publications and Presentations

Substantial uncertainties still exist in the scientific understanding of the possible interactions between urban and natural (biogenic) emissions in the production and transformation of atmospheric aerosol and the resulting impact on climate change. The US Department of Energy (DOE) Atmospheric Radiation Measurement (ARM) program’s Carbonaceous Aerosol and Radiative Effects Study (CARES) carried out in June 2010 in Central Valley, California, was a comprehensive effort designed to improve this understanding. The primary objective of the field study was to investigate the evolution of secondary organic and black carbon aerosols and their climate-related properties in the Sacramento urban plume as it was …

Review Of Dust-Up: Asbestos Litigation And The Failure Of Commonsense Policy Reform By Jeb Barnes, Paul Nolette

Political Science Faculty Research and Publications

No abstract provided.

An Empirical Examination Of University Intercollegiate Athletic Expenditures, Jeffery L. Stinson, Adam Marquardt, Joshua Chandley

Marketing Faculty Publications

To date, little empirical work has examined the institutional returns associated with athletic program investments. While intangible brand effects are commonly cited, such as athletics serving as the perceptual "front porch" of the institution, direct examination of the effects of athletic programs has often been narrow in scope. Within this study, we assess the contributions of investment in athletics as compared to other areas of institutional investment, on important institutional outcomes. Data for the study was collected from two datasets, the Integrated Postsecondary Education Data System (IPEDS) and the Equity in Athletics dataset. Fixed effects models for NCAA Football Bowl …

The Newest 'War On Drugs:' Regulating Pharmaceuticals Through State Litigation, Paul Nolette

Political Science Faculty Research and Publications

State-driven litigation has had increasing influence in the development of national policy in recent years, including in national health policy. One prominent recent example includes the efforts of several state governments to bring coordinated constitutional challenges against one of the Obama Administration’s key first term achievements, the Patient Protection and Affordable Care Act. This paper examines how states have influenced health care policy influence in a more subtle but no less important litigation campaign. Over the past decade, state prosecutors have reached numerous multi-million dollar settlements with the nation’s largest pharmaceutical companies imposing a variety of restrictions on prescription drug …

Recent Applications Of The Simple Hydrocarbon Cyclooctatetrene As A Starting Material For Complex Molecule Synthesis, Kevin W. Glaeske, William A. Donaldson

Chemistry Faculty Research and Publications

Cyclooctatetraene [COT], a simple non-aromatic cyclic polyene, is capable of undergoing a variety of oxidation and cycloaddition reactions to afford polycyclic structures. In addition, complexation of COT or the cycloaddition products with transition metals facilitates bond formation. Recent developments in the reactivity of COT and application to the synthesis of naturally occurring and non-naturally occurring compounds is reviewed.

Nmr Line Shapes And Multi-State Binding Equilibria, Evgenii L. Kovrigin

Chemistry Faculty Research and Publications

Biological function of proteins relies on conformational transitions and binding of specific ligands. Protein-ligand interactions are thermodynamically and kinetically coupled to conformational changes in protein structures as conceptualized by the models of pre-existing equilibria and induced fit. NMR spectroscopy is particularly sensitive to complex ligand-binding modes—NMR line-shape analysis can provide for thermodynamic and kinetic constants of ligand-binding equilibria with the site-specific resolution. However, broad use of line shape analysis is hampered by complexity of NMR line shapes in multi-state systems. To facilitate interpretation of such spectral patterns, I computationally explored systems where isomerization or dimerization of a protein (receptor) molecule …

Pyridine Is An Organocatalyst For The Reductive Ozonolysis Of Alkenes, Rachel Willand-Charnley, Thomas J. Fisher, Bradley M. Johnson, Patrick Dussault

Patrick Dussault Publications

Whereas the cleavage of alkenes by ozone typically generates peroxide intermediates that must be decomposed in an accompanying step, ozonolysis in the presence of pyridine directly generates ketones or aldehydes through a process that neither consumes pyridine nor generates any detectable peroxides. The reaction is hypothesized to involve nucleophile-promoted fragmentation of carbonyl oxides via formation of zwitterionic peroxyacetals.

Why Proportionality Matters, Youngjae Lee

Faculty Scholarship

No abstract provided.

Do Boumediene Rights Expire?, Andrew Kent

Faculty Scholarship

In 2008, Guantanamo detainees won a landmark victory in Boumediene v. Bush, which held that the Congress and the President could not prevent the detainees from accessing the courts to seek release via habeas corpus. The Court decided that persons claiming to be innocent civilians deserved a day in court, even though they were noncitizens held by the U.S. military as enemy combatants on foreign territory. The Court applied a fact-specific test that granted habeas rights to noncitizens outside the United States only when a balance of factors — including citizenship, enemy status, the nature of status review procedures, the …

Patient Racial Preferences And The Medical Culture Of Accommodation, Kimani Paul-Emile

Faculty Scholarship

One of medicine’s open secrets is that patients routinely refuse or demand medical treatment based on the assigned physician’s racial identity, and hospitals typically yield to patients’ racial preferences. This widely practiced, if rarely acknowledged, phenomenon — about which there is new empirical evidence — poses a fundamental dilemma for law, medicine, and ethics. It also raises difficult questions about how we should think about race, health, and individual autonomy in this context. Informed consent rules and common law battery dictate that a competent patient has an almost-unqualified right to refuse medical care, including treatment provided by an unwanted physician. …

Notes Toward A Critical Contemplation Of Law, Sonia K. Katyal

Faculty Scholarship

In this tribute to Professor Derrick Bell’s legacy, Professor Katyal reflects on one of Bell’s greatest gifts: the necessary, and perhaps unfinished gift of critical contemplation of law, along with its possibilities and its concomitant limitations. In her paper, Katyal reflects on two seemingly disparate areas of civil rights that might benefit from Bell’s critical vision: the area of LGBT rights and equality, and federal Indian law. Relying on some of Bell’s most valuable insights, Katyal calls for the creation of a “critical sexuality studies” and a “critical indigenous studies” that employs some of Bell’s groundbreaking lessons in reimagining broader …

Annual Report: 2012, Centracare Health

Annual Report

A review of the organizations performance from the 2012 fiscal year

Material And Motion: Phenomenology And The Early Work Of Carolee Schneemann 1957-1973, Regina M. Flowers

School of Art, Art History, and Design: Theses and Student Creative Work

Carolee Schneemann is a multidisciplinary artist known for using her body in her artworks in order to engage with issues of sexuality, gender and identity. Best known for her 1975 performance Interior Scroll, Schneemann’s work is most often theorized in connection with the emergence of Feminist, Performance and Body Art, yet Schneemann has always considered herself primarily a painter. In this thesis I address the disconnect between Schneemann’s repeated insistence on her status as a painter and the scholarly discussion of her work solely in relation to the integration of her body in her performative works. The period covered …

Envisioning Enforcement Of Freedom Of Association Standards In Corporate Codes: A Journey For Sinbad Or Sisyphus?, James J. Brudney

Faculty Scholarship

Since the 1970’s, multinational corporations (MNCs) in large numbers have adopted codes of conduct declaring their commitment to workers’ rights. These codes, however, do not require adherence to specific labor regulations or standards in a global setting. The MNC record on voluntary compliance has been discouraging, especially in labor-intensive industries like apparel, shoes, and toys, where a global supply chain of contractors effectively controls labor conditions. The persistent gap between aspiration and achievement regarding corporate codes has led to disagreement over their meaning and value. MNCs hope to be judged on the basis of the self-regulatory systems they have established. …

Distrust And Clarify: Appreciating Congressional Overrides, James J. Brudney

Faculty Scholarship

Deborah Widiss continues to make important contributions in an area of statutory interpretation that has been largely neglected: the consequences of congressional overrides. Professor Widiss previously demonstrated how the Supreme Court and lower courts often confine the reach of statutes that purposefully override prior court decisions, thereby reviving aspects of the overridden judicial interpretations as ―shadow precedents.‖ In Undermining Congressional Overrides: The Hydra Problem in Statutory Interpretation, Professor Widiss addresses the Supreme Court‘s further expansion of judicial power in the aftermath of congressional disapproval. Faced with the override of its textual interpretation in one employment discrimination statute, the Court inferred …

Networks, Dialogue Or One-Way Traffic? An Empirical Analysis Of Cross-Citations Between Ten European Supreme Courts, Martin Gelter, Mathias Siems

Faculty Scholarship

No abstract provided.

Tensions In Rhetoric And Reality At The Intersection Of Work And Immigration, Jennifer Gordon

Faculty Scholarship

No abstract provided.

Lineage-Specific Differences Between Human And Simian Immunodeficiency Virus Regulation Of Gp120 Trimer Association And Cd4 Binding, Andrés Finzi, Beatriz Pacheco, Shi-Hua Xiang, Marie Pancera, Alon Herschhorn, Liping Wang, Xing Zeng, Anik Desormeaux, Peter D. Kwong, Joseph Sodroski

Nebraska Center for Virology: Faculty Publications

Metastable conformations of the gp120 and gp41 envelope glycoproteins of human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV) must be maintained in the unliganded state of the envelope glycoprotein trimer. Binding of gp120 to the primary receptor, CD4, triggers the transition to an open conformation of the trimer, promoting interaction with the CCR5 chemokine receptor and ultimately leading to gp41-mediated virus-cell membrane fusion and entry. Topological layers in the gp120 inner domain contribute to gp120-trimer association in the unliganded state and to CD4 binding. Here we describe similarities and differences between HIV-1 and SIVmac gp120. In both …

Subunit Organization Of The Membrane-Bound Hiv-1 Envelope Glycoprotein Trimer, Youdong Mao, Liping Wang, Christopher Gu, Alon Herschhorn, Shi-Hua Xiang, Hillel Haim, Xinzhen Yang, Joseph Sodroski

Nebraska Center for Virology: Faculty Publications

The trimeric human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env) spike is a molecular machine that mediates virus entry into host cells and is the sole target for virus-neutralizing antibodies. The mature Env spike results from cleavage of a trimeric gp160 precursor into three gp120 and three gp41 subunits. Here we describe an ~11-Å cryo-EM structure of the trimeric HIV-1 Env precursor in its unliganded state. The three gp120 and three gp41 subunits form a cage-like structure with an interior void surrounding the trimer axis. Interprotomer contacts are limited to the gp41 transmembrane region, the torus-like gp41 ectodomain, and …

A Detrimental Soil Disturbance Prediction Model For Ground-Based Timber Harvesting, Derrick A. Reeves, Matthew C. Reeves, Ann M. Abbott, Deborah S. Page-Dumroese, Mark D. Coleman

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Soil properties and forest productivity can be affected during ground-based harvest operations and site preparation. The degree of impact varies widely depending on topographic features and soil properties. Forest managers who understand site-specific limits to ground-based harvesting can alter harvest method or season to limit soil disturbance. To determine the potential areal extent of detrimental (potentially plant growth limiting) soil disturbance based on site characteristics and season of harvest, we developed a predictive model based on soil monitoring data collected from 167 groundbased harvest units. Data collected included dominant site parameters (e.g., slope, aspect, soil texture, and landtype), harvest season, …

Fish And Other Aquatic Resource Trends In The United States A Technical Document Supporting The Forest Service 2010 Rpa Assessment, Andrew J. Loftus, Curtis H. Flather

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

The Forest and Rangeland Renewable Resources Planning Act (RPA) of 1974 requires periodic assessments of the status and trends in the Nation’s renewable natural resources including fish and other aquatic species and their habitats. Data from a number of sources are used to document trends in habitat quality, populations, resource use, and patterns of imperilment among aquatic fauna. Freshwater habitat quality varied widely across the United States. Nationwide, more than half of monitored lakes were ranked in good condition, but the percentage ranged from a high of 91 percent in the upper Midwest to a low of 1 percent in …

A Synoptic Review Of U.S. Rangelands A Technical Document Supporting The Forest Service 2010 Rpa Assessment, Matthew Clark Reeves, John E. Mitchell

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

The Renewable Resources Planning Act of 1974 requires the USDA Forest Service to conduct assessments of resource conditions. This report fulfills that need and focuses on quantifying extent, productivity, and health of U.S. rangelands. Since 1982, the area of U.S. rangelands has decreased at an average rate of 350,000 acres per year owed mostly to conversion to agricultural and residential land uses. Nationally, rangeland productivity has been steady over the last decade, but the Rocky Mountain Assessment Region appears to have moderately increased productivity since 2000. The forage situation is positive and, from a national perspective, U.S. rangelands can probably …

Nebraska’S Forest Resources, 2012, Dacia M. Meneguzzo, Chuck Barnett

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

This publication provides an overview of forest resource attributes for Nebraska based on an annual inventory conducted by the Forest Inventory and Analysis (FIA) program at the Northern Research Station of the U.S. Forest Service. These estimates, along with web-posted core tables, will be updated annually. For more information please refer to page 5 of this report.