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A Conserved Two-Component Regulatory System, Pids/Pidr, Globally Regulates Pigmentation And Virulence-Related Phenotypes Of Burkholderia Glumae, Hari Sharan Karki, Inderjit Kaur Barphagha, Jong Hyun Ham

Faculty Publications

Burkholderia glumae is a rice pathogenic bacterium that causes bacterial panicle blight. Some strains of this pathogen produce dark brown pigments when grown on casamino-acid peptone glucose (CPG) agar medium. A pigment-positive and highly virulent strain of B.glumae, 411gr-6, was randomly mutagenized with mini-Tn5gus, and the resulting mini-Tn5gus derivatives showing altered pigmentation phenotypes were screened on CPG agar plates to identify the genetic elements governing the pigmentation of B.glumae. In this study, a novel two-component regulatory system (TCRS) composed of the PidS sensor histidine kinase and the PidR response regulator was identified as an essential regulatory factor for pigmentation. Notably, …

Inferring The Evolutionary History Of Divergence Despite Gene Flow In A Lizard Species, Scincella Lateralis (Scincidae), Composed Of Cryptic Lineages, Nathan D. Jackson, Christopher C. Austin

Faculty Publications

Although recent radiations are fruitful for studying the process of speciation, they are difficult to characterize and require the use of multiple loci and analytical methods that account for processes such as gene flow and genetic drift. Using multilocus sequence data, we combine hierarchical cluster analysis, coalescent species tree inference, and isolation-with-migration analysis to investigate evolutionary relationships among cryptic lineages of North American ground skinks. We also estimate the extent that gene flow has accompanied or followed diversification, and also attempt to account for and minimize the influence of gene flow when reconstructing relationships. The data best support seven largely …

Sperm Cryopreservation In Live-Bearing Xiphophorus Fishes: Offspring Production From Xiphophorus Variatus And Strategies For Establishment Of Sperm Repositories, Huiping Yang, Rafael Cuevas-Uribe, Markita G. Savage, Ronald B. Walter, Terrence R. Tiersch

Faculty Publications

Cryopreservation of sperm from Xiphophorus fishes has produced live young in three species: X. hellerii, X. couchianus, and X. maculatus. In this study, the goal was to establish protocols for sperm cryopreservation and artificial insemination to produce live young in X. variatus, and to identify needs for repository development. The objectives were to: 1) collect basic biological characteristics of males; 2) cryopreserve sperm from X. variatus, 3) harvest live young from cryopreserved sperm, and 4) discuss the requirements for establishment of sperm repositories. The 35 males used in this study had a body weight of 0.298±0.096g (mean±SD), body length of …

Beyond Theatres : Issue 2, 2012, Department Of Surgery

Department of Surgery Archives

No abstract provided.

First Choice, September 2012, Wusf Public Media

First Choice Monthly Newsletter

The September 2012 issue of WUSF's newsletter "First Choice."

In The Language Of Pictures: How Copyright Law Fails To Adequately Account For Photography, Teresa M. Bruce

West Virginia Law Review

No abstract provided.

Borrelia Burgdorferi Cp32 Bpab Modulates Expression Of The Prophage Nucp Nuclease And Ssbp Single-Stranded Dna-Binding Protein, Alicia M. Chenail, Brandon L. Jutras, Claire A. Adams, Logan H. Burns, Amy Bowman, Ashutosh Verma, Brian Stevenson

Microbiology, Immunology, and Molecular Genetics Faculty Publications

The Borrelia burgdorferi BpaB proteins of the spirochete's ubiquitous cp32 prophages are DNA-binding proteins, required both for maintenance of the bacteriophage episomes and for transcriptional regulation of the cp32 erp operons. Through use of DNase I footprinting, we demonstrate that BpaB binds the erp operator initially at the sequence 5′-TTATA-3′. Electrophoretic mobility shift assays indicated that BpaB also binds with high affinity to sites located in the 5′ noncoding regions of two additional cp32 genes. Characterization of the proteins encoded by those genes indicated that they are a single-stranded DNA-binding protein and a nuclease, which we named SsbP and NucP, …

"As A Bird Sings": Hannah Tapfield King, Poetess And Pioneer, Leonard Reed

BYU Studies

Hannah Tapfield King (1807-1886), converted to The Church of Jesus Christ of Latter-day Saints in 1850 in Cambridge, England, and emigrated with her family to Utah. She was a prolific and popular writer of poetry, essays, and educational pieces for Utah's newspapers, and she had a reputation as a woman of refinement. From her autobiography one can see her complete devotion to the Church. She and her family sacrificed a comfortable middle class life in England running the family farm, and they suffered considerably in Utah's desert. Her husband, Thomas King, eventually joined the Church but was never active or …

Nauvoo Neighbor: The Latter-Day Saint Experience At The Mississippi River, 1843–1845, Susan E. Black

BYU Studies

This excerpt from Susan Easton Black's new book introduces the Mormon newspaper the Nauvoo Neighbor and the significant role it played in Nauvoo between 1843 and 1845.

Law In A Plural Society: Malaysian Experience, Zaki Azmi

BYU Law Review

No abstract provided.

Ask, Tell, But Do Not Get Greedy: The Inequalities That Pervade In The Military In Light Of The Repeal Of “Don’T Ask, Don’T Tell”, David Barnes

Washington and Lee Journal of Civil Rights and Social Justice

No abstract provided.

Variation In Genes Related To Cochlear Biology Is Strongly Associated With Adult-Onset Deafness In Border Collies, Jennifer S. Yokoyama, Ernst T. Lam, Alison L. Ruhe, Carolyn A. Erdman, Kathryn R. Robertson, Aubrey A. Webb, D. Colette Williams, Melanie L. Chang, Marjo K. Hytönen, Hannes Lohi, Steven P. Hamilton, Mark W. Neff

Anthropology Faculty Publications and Presentations

The domestic dog offers a unique opportunity to study complex disorders similar to those seen in humans, but within the context of the much simpler genetic backgrounds of pure breeds, which represent closed populations. We performed a whole-genome search for genetic risk factors of adult-onset deafness in the Border Collie, a breed of herding dog that relies on acute hearing to perceive and respond to commands while working. Adult-onset deafness in Border Collies typically begins in early adulthood and is similar to age-related hearing loss in humans. This earlier onset has particular impact on the utility of working Border Collies …

Maximal Force Characteristics Of The Ca2+-Powered Actuator Of Vorticella Convallaria, Sangjin Ryu, Matthew J. Lang, Paul Matsudaira

Department of Mechanical and Materials Engineering: Faculty Publications

The millisecond stalk contraction of the sessile ciliate Vorticella convallaria is powered by energy from Ca²⁺ binding to generate contractile forces of ~10 nN. Its contractile organelle, the spasmoneme, generates higher contractile force under increased stall resistances. By applying viscous drag force to contracting V. convallaria in a microfluidic channel, we observed that the mechanical force and work of the spasmoneme depended on the stalk length, i.e., the maximum tension (150–350 nN) and work linearly depended on the stalk length (~2.5 nN and ~30 fJ per 1 mm of the stalk). This stalk-length dependency suggests that motor units of …

Endogenous Inhibitor Proteins That Connect Ser/Thr Kinases And Phosphatases In Cell Signaling., Masumi Eto, David L Brautigan

Department of Molecular Physiology and Biophysics Faculty Papers

Protein phosphatase activity acts as a primary determinant of the extent and duration of phosphorylation of cellular proteins in response to physiological stimuli. Ser/Thr protein phosphatase-1 (PP1) belongs to the PPP superfamily, and is associated with regulatory subunits that confer substrate specificity, allosteric regulation, and subcellular compartmentalization. In addition, all eukaryotic cells contain multiple heat-stable proteins that originally were thought to inhibit phosphatase catalytic subunits released from the regulatory subunits, as a fail-safe mechanism. However, discovery of C-kinase-activated PP1 inhibitor, Mr of 17 kDa (CPI-17) required fresh thinking about the endogenous inhibitors as specific regulators of particular phosphatase complexes, acting …

Center For Quantitative And Qualitative Research Methods, Todd C. Headrick, Rhonda K. Kowalchuk, Yanyan Sheng, Jennifer Koran

Reports

No abstract provided.

Southern Alumni, Siu Alumni Association

SIU Alumni Magazine

No abstract provided.

Personal Identity And Nostalgia For The Distant Land Of Past: Legacy Tourism, Nina M. Ray, Gary Mccain

Marketing Faculty Publications and Presentations

"The past is certainly a distant land and getting there is a difficult and imperfect undertaking" (Brown, Hirschman & Maclaran (2006). This paper explores motivations behind how consumers reach that "distant land." Over 1,000 respondents of a variety of ethnic groups show very different stories and diaspora timelines, but personal identity and connection with place are always top ranked motivations for interest in ancestors. How might groups, who may suffer from a lack of identity, fit into these findings when 'personal identity' is the number one reason why consumers engage in genealogy and legacy tourism? Whether a group is well …

Between Heaven And Earth: Christian Perspectives On Environmental Protection (Book Review), Jeff Ploegstra

Pro Rege

Reviewed Title: Van Dyke, Fred. Between Heaven and Earth: Christian Perspectives on Environmental Protection. Santa Barbara: Praeger, 2010. 237 pages. ISBN 978-0-313-37536-1.

Twentieth-Century Catalogs: The Poetics Of Listing, Enumeration, And Copiousness In Joyce, Schuyler, Mccourt, Pynchon, And Perec, Timothy Krause

Dissertations, Theses, and Capstone Projects

This dissertation examines the occurrence of catalogs and lists in the literary works of several twentieth-century authors, including James Joyce, poet James Schuyler, novelist and cultural historian James McCourt, the postmodern fabulist Thomas Pynchon, and the French experimental prose author Georges Perec. The dissertation seeks to trace how each author makes use of catalogs in his work, how catalogs form a central part of his style and subject matter, and how his use of catalogs can be read against the biographical, historical, and social contexts surrounding his life and work. A theoretical introduction situates my work among theorists of epistemology, …

Safety Evaluations Of Ethanolic Extract Of Moringa Oleifera Lam. Seed In Experimental Animals, Songpol Chivapat, Pornchai Sincharoenpokai, Praw Suppajariyawat, Anudep Rungsipipat, Suphan Phattarapornchaiwat, Vanida Chantarateptawan

The Thai Journal of Veterinary Medicine

The safety of ethanolic extract from the seeds of Moringa oleifera was evaluated for its oral toxicity by acute toxicity test and subchronic toxicity study in experimental animals. The acute toxicity test in mice showed that the extract at doses ranging from 5.1 to 10.0 g/kg caused toxic signs and dose dependent mortality. Oral administration of the extract in rats at doses of 100, 500 and 1000 mg/kg/day for 90 consecutive days revealed that male rats treated with the extract at 1000 mg/kg had a significant decrease in RBC but this alteration was within rats reference range. A significant decrease …

Experimental Infection Of Mice And Baby Chickens With Thailand Strain Of Chikungunya Virus, Sonthaya Tiawsirisup, Pichanan Rattanakampol, Warritar Navavichit, Hemmarat Ratpiyapaporn

The Thai Journal of Veterinary Medicine

This study was conducted to investigate Chikungunya virus (CHIKV) infection in mammal and avian byusing mice and baby chickens as model animals. Thailand 2010 and Ross/186 strain (reference strain) were used inthis study. 104, 106 and 108 CID50 of CHIKV were inoculated to four- and six-week-old mice. Blood was collected andtested for virus for seven days. 108 CID50 of CHIKV was inoculated to two-week-old mice. Blood was collected andtested for virus for five days. For the baby chickens, 102, 104, 106 and 108 CID50 of CHIKV were inoculated to five-dayoldbaby chickens. Blood was collected and tested for virus for seven …

Five Year Issue-Based Review: September 2012. A Comprehensive Study Of The Southeast District Of University Of Nebraska–Lincoln Extension

Southeast Research and Extension Center: Reports and Administration

CONTENTS

1. Vision and Overview ... 5-8

2. Next Generation Extension ... 9

a New Delivery Systems ... 11-14

b Measuring Return on Investments ... 15-19

c Partnerships ... 20-23

d Translational Research ... 24-29

e Staying on the Cutting Edge ... 30-31

3. Agriculture and Science Literacy ... 33-34

a Livestock ... 35-39

b Crops ... 40-43

c Water ... 44-47

d Nutrition ... 48-51

4. SREC Contributions: ... 53

IANR Growing a Healthy Future – 2025 Goals

a Food

Crops ... 55-59

Nutrition ... 60-64

Livestock ... 65-66

b Water

Crops & Urban ... 67-68

c People

4-H …

Plains Harvest Mouse (Reithrodontomys Montanus Griseus): Species Conservation Assessment, Melissa J. Panella

Nebraska Game and Parks Commission: Publications

The primary goal in development of at-risk species conservation assessments is to compile biological and ecological information that may assist conservation practitioners in making decisions regarding species of interest. The Nebraska Natural Legacy Project recognizes the plains harvest mouse (Reithrodontomys montanus griseus) as a Tier I at-risk species of high conservation need. Some general management recommendations are made here regarding the plains harvest mouse; however, conservation practitioners will need to use professional judgment to make specific management decisions based on objectives, location, and a multitude of variables. This resource was designed to share available knowledge of the plains harvest mouse …

Topeka Shiner (Notropis Topeka): Species Conservation Assessment, Melissa J. Panella

Nebraska Game and Parks Commission: Publications

The primary goal in development of at-risk species conservation assessments is to compile biological and ecological information that may assist conservation practitioners in making decisions regarding the conservation of species of interest. The Nebraska Natural Legacy Project recognizes the Topeka shiner (Notropis topeka) as a Tier I at-risk species of high priority for conservation. Some general management recommendations are made here regarding Topeka shiners; however, conservation practitioners will need to use professional judgment to make specific management decisions based on objectives, location, and a multitude of variables. This resource was designed to share available knowledge of the Topeka shiner that …

Neeg Tawg Rog (War-Torn People): Linguistic Consciousness In The Hmong Diaspora, Geraldine Craig

Textile Society of America: Symposium Proceedings

This paper examines how new Hmong textile forms that started in Ban Vinai refugee camp have served as dialogical performance, spiritual and political agency, and transnational commodity in the diasporas. These textiles create a hybrid social language that draws from a diversity of linguistic consciousnesses in a culture where there was not a universally understood writing system until the mid-20th century. Traditional paj ntaub (flower cloth) garments in Laos were a primary indicator of Hmong identity and can be seen as an alternate text. The complex, layered geometric patterns in paj ntaub established ethnic group identification and offered passive resistance …

The Wild Civilized: An Environmental Art Studio Installed Outdoors In An Institutional Space, Neil Goss

Textile Society of America: Symposium Proceedings

In 2011, I set up an outdoor dyeing and weaving studio that also functioned as a sculptural installation, The Earthway Studio. The purpose was multi-dimensional: to educate the university community and the public about culturally pluralistic fiber processes such as spinning, dyeing and weaving, to glean and process a bulk of my materials on site and to inspire others through demonstrations and performances to ignite a relationship with the land and to offer the concept of tolerance for diversity when looking at different approaches to living life. The purpose of this conference presentation is to share the experience of the …

Sogdian Textile Design: Political Symbols Of An Epoch, Elmira Gyul

Textile Society of America: Symposium Proceedings

Sogdian fabrics produced in Central Asia became one of the shining symbols of the Early Middle Ages, characterized by the blossoming of city cultures of the region, the broad international contacts created by the emergence of the Great Silk Road, and the active interaction of urban and nomadic societies. These silk fabrics were highly appreciated for their quality and variety of design. As a rule, the designs of Sogdian fabrics were considered from the point of view of their cult or religious character. The general high demand of these fabrics in the countries of both the West and the East, …

Political Voices Of Three Left Coast Artists: Gyongy Laky, Linda Gass, Linda Macdonald, Barbara Shapiro

Textile Society of America: Symposium Proceedings

Northern Californian has a long history of protest and political involvement. For Linda Gass, Gyongy Laky and Linda MacDonald the passion they bring to political issues is reflected in their artwork and the paths their lives have followed. Based on personal interviews and research, this paper will shed light on their views and the artistic means used to express them. Each of these artists is engaged in political activism in her own way. In each case we are drawn in by the form, beauty or humor of the textiles. Closer inspection makes us think deeply about significant political issues. Linda …

Ink (2012-09)

Ink, 2008-

The student-run features magazine covering the fashion, art, music and culture worlds at VCU and in Richmond. Published annually in print and updated regularly online.

A Man's World: Revisiting Histories Of Men And Gender: Review Of "On The Make: Clerks And The Quest For Capital In Nineteenth-Century America; Jolly Fellows: Male Milieus In Nineteenth-Century America" By B.P. Luskey And R. Stott, Bruce Dorsey

History Faculty Works

No abstract provided.