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The Occurrence Of An Abdominal Fauna In An Articulated Tapir (Tapirus Polkensis) Recovered From The Miocene Gray Fossil Site Northeast Tennessee., Shannon M. Mcconnell

Electronic Theses and Dissertations

The analysis of samples recovered from the abdominal area of an articulated tapir from the Late Miocene (4.5-7 million bp) Gray Fossil Site, revealed a rich palyno-fauna comprised of about 94% egg/oocyst-like structures and 6% pollen and other palynomorphs. In addition, a tight grouping of six hickory nuts (Carya) was recovered from the same area suggesting that the samples represent the abdominal contents. The analysis of a sample from immediately outside the tapir produced a sample with 98% pollen and less than 0.5% egg-like structures. The size, shape, and general morphology of egg-like structures were analyzed with light …

'Reflection' And Other Stories, Melissa Turner

All Theses

A creative thesis made up of four fiction stories. The stories focus on identity within the major characters and how they cope with the circumstances of life. 'Reflection and Other Stories' is realistic in plot and style.

Epigeic Spider (Araneae) Diversity And Habitat Distributions In Kings Mountain National Military Park, South Carolina, Sarah Stellwagen

All Theses

This study examined the epigeic spider fauna in Kings Mountain National Military Park. The aim of this study is to make this information available to park management for use in the preservation of natural resources. Pitfall trapping was conducted monthly for one year in three distinct habitats: riparian, forest, and ridge-top. The study was conducted from August 2009 to July 2010. One hundred twenty samples were collected in each site. Overall, 289 adult spiders comprising 66 species were collected in the riparian habitat, 345 adult comprising 57 species were found in the forest habitat, and 240 adults comprising 47 species …

Multi-Scale Modeling Of The Fontan Circulation Using A Mock Circulatory System, John Chiulli

All Theses

The Fontan circulation is the result of a series of operations performed to save the lives of children born with univentricular circulations. The Fontan procedure achieves venous return to the pulmonary circulation without a ventricular power source. The load on the heart is reduced to normal, and these patients can lead a normal life into adulthood, although late complications continue to prevent normal lifespan. A unique feature of the Fontan circulation is the dependency of inferior vena cava flow on respiration. The Fontan circulation has been modeled experimentally using an adjustable mock circulatory system, which for the first time includes …

How Do Planners Participate In Formulating Viable Green Infrastructure Plans?, Kaylen Francis

All Theses

The planning field is increasingly involved in balancing biodiversity with the expansion of urban areas to meet changing societal demands. Regions, states and cities have begun trying to mitigate the negative effects of sprawl by creating green infrastructure plans to conserve open space. However, little is known about planners' roles in facilitating and/or implementing these visions in cities with a population of over one million people in the core and hinterlands. The goal of this project was to study the Chicago Wilderness Green Infrastructure Vision to determine if planners played a role in its formulation and/or implementation, and if so, …

Acclerated Vehicle Retirement: Toward A Conceptualized Framework For Design And Implementation, Nathan Woods

All Theses

ABSTRACT
In the past Accelerated Vehicle Retirement (AVR) programs have been implemented to combat the economic and environmental costs of automobile dependent societies. Seventy-five such programs have been implemented worldwide since 1990. This thesis examines correlations among factors affecting program performance, the relationship of articulated program objectives to program performance, and how factors affecting program performance influence environmental concerns. Employing a mixed methodology, this analysis answers how an accelerated vehicle retirement program can be designed to maximize desired outcomes and minimize undesirable outcomes. The results of this analysis demonstrate that the order and type of objective stipulated by a program …

Assessment Of Conceptual Understanding Of Atomic Structure, Covalent Bonding, And Bond Energy, Alma Gonzales

All Theses

This research work relied upon the importance of conceptual understanding to meaningful learning. Conceptual understanding is strongly influenced by students' prior knowledge (1). Many of these ideas pose strong barriers to deeper understanding and are called misconceptions since they are inconsistent or in conflict with generally accepted scientific facts (2). Thus, it is beneficial for instructors to initially elicit students' conceptual understanding in order to properly address student's misconceptions during the learning process. However, results are very dependent on the instrument used to elicit conceptual understanding (3). The most commonly used tools are summative assessments or achievement tests (4). They …

A Study Of The Biltmore Estate's Ramble: Researching Design Intent, Evaluating Change Over Time, And Proposing A Rehabilitation Plan, Owen Mclaughlin

All Theses

The purpose of this study is to propose a historically appropriate rehabilitation plan for the Biltmore Estate's Ramble based upon Olmsted's original design intent. In order to ascertain Frederick Law Olmsted's original design intent for the Ramble, an extensive review of Victorian Era garden design, cultural landscapes, the U.S. Horticultural Industry, and possible design influences on Olmsted as he designed the site was performed. Using primary archival materials and secondary sources, the developmental history of the Ramble from 1890 to present was studied. From that work, design intent was determined by analyzing character-defining features from the original 1893 planting plan …

Evaluating The Impacts Of Production Process And Product Origin On Willingness To Pay For Shrimp, Addison Ellis

All Theses

The 2002 Farm Bill included a provision requiring various agricultural commodities to comply with country of origin labeling (COOL). Proponents of COOL argued that the policy would support U.S. producers and benefit U.S. consumers. This argument was based on the assumption that U.S. consumers prefer domestic products to imported products and hence the demand for U.S. products would increase following the implementation of COOL. However, analyzing the impact of COOL on shrimp, one of the first commodities covered the program, is complicated by recent trade disputes, concerns over the safety of farm raised products and potential stigma effects associated with …

Simultaneous Optimization Of Macronutrient Factors In Plant Tissue Culture Using Turmeric (Curcuma Longa L.) As A Model, Sean Halloran

All Theses

Murashige and Skoog 1962 performed one-factor-at-a-time (OFAT) experimentation in order to regenerate tobacco callus on semi-solid agar medium. This work became an established medium for tissue culture micropropagation and experimentation. Micropropagation is done in niche markets with herbaceous perennials, among other crops, and the optimization of various inputs to produce maximal responses is a necessary step towards process development. This current study of macronutrient factors simultaneously altered media volume, amount of tissue (plants per vessel), sucrose, nitrogen (as NO3- and NH4+ ions), and K+ in a d-optimal design space with only 55 experimental units (including 5 true replicates). The first …

Application Of Gis And Geographically Weighted Regression To Evaluate The Spatial Non‐Stationarity Relationships Between Precipitation Vs. Irrigated And Rainfed Maize And Soybean Yields, Vivek Sharma, Ayse Kilic, Isa Kabenge, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Understanding the relationship between the spatial distribution of precipitation and crop yields on large scales (i.e., county, state, regional) while accounting for the spatial non‐stationarity can help managers to better evaluate the long‐term trends in agricultural productivity to make better assessments in food security, policy decisions, resource assessments, land and water resources enhancement, and management decisions. A relatively new technique, geographically weighted regression (GWR), has the ability to account for spatial non‐stationarity with space. While its application is growing in other scientific disciplines (i.e., social sciences), the application of this new technique in agricultural settings has not been practiced. The …

Narrow Grass Hedge Control Of Nutrient Loads Following Variable Manure Applications, John E. Gilley, Lisa M. Durso, Roger Eigenberg, David B. Marx, Brian L. Woodbury

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The effectiveness of a narrow grass hedge in reducing runoff nutrient loads following manure application was examined in this study. Beef cattle manure was applied to 0.75 m wide by 4.0 m long plots established on an Aksarben silty clay loam located in southeast Nebraska. Manure was added at rates required to meet none or the 1, 2, or 4 year nitrogen requirements for corn. Runoff water quality was measured during three 30 min simulated rainfall events. Manure application rate significantly affected dissolved phosphorus (DP) and total phosphorus (TP) loads in runoff on the plots without a grass hedge. However, …

Enigmatic Ornamentation Eases Male Reliance On Courtship Performance For Mating Success, Eileen Hebets, Jay A. Stafstrom, Rafael L. Rodriguez, Dustin J. Wilgers

Eileen Hebets Publications

Female preferences are frequently invoked to explain the widespread occurrence of elaborate male ornaments, yet empirical data demonstrating such preferences are sometimes equivocal or even contradictory. In the wolf spider Schizocosa stridulans, despite evidence of strong female choice, prior research has been unable to link the conspicuous sexually dimorphic foreleg ornamentation of males to their mating success. We conducted three experiments aimed at determining the function of this previously enigmatic ornamentation. Our first two experiments used males with phenotypically modified foreleg phenotypes in simple and complex mating environments in order to examine the relationship between the presence/absence of ornamentation …

Expression And Purification Of Recombinant Hemoglobin In Escherichia Coli, Chandrasekhar Natarajan, Xiaoben Jiang, Angela Fago, Roy E. Weber, Hideaki Moriyama, Jay F. Storz

Jay F. Storz Publications

Background: Recombinant DNA technologies have played a pivotal role in the elucidation of structure-function relationships in hemoglobin (Hb) and other globin proteins. Here we describe the development of a plasmid expression system to synthesize recombinant Hbs in Escherichia coli, and we describe a protocol for expressing Hbs with low intrinsic solubilities. Since the alpha- and beta-chain Hbs of different species span a broad range of solubilities, experimental protocols that have been optimized for expressing recombinant human HbA may often prove unsuitable for the recombinant expression of wildtype and mutant Hbs of other species.

Methodology/Principal Findings: As a test case …

Phylogenetic Diversification Of The Globin Gene Superfamily In Chordates, Jay F. Storz, Juan C. Opazo, Federico G. Hoffmann

Jay F. Storz Publications

Phylogenetic reconstructions provide a means of inferring the branching relationships among members of multigene families that have diversified via successive rounds of gene duplication and divergence. Such reconstructions can illuminate the pathways by which particular expression patterns and protein functions evolved. For example, phylogenetic analyses can reveal cases in which similar expression patterns or functional properties evolved independently in different lineages, either through convergence, parallelism, or evolutionary reversals. The purpose of this article is to provide a robust phylogenetic framework for interpreting experimental data and for generating hypotheses about the functional evolution of globin proteins in chordate animals. To do …

Host And Distribution Lists Of Chiggers (Trombiculidae And Leeuwenhoekiidae), Of North American Wild Vertebrates North Of Mexico, Brianne L. Walters, John O. Whitaker Jr., Nicholas S. Gikas, William J. Wrenn

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Information concerning chiggers found on wild vertebrates of North America north of Mexico is summarized. Included are lists a) of the chiggers organized taxonomically, b) hosts from which each species has been reported, and c) states and provinces with references for each separate record

Mitostatin Is Down-Regulated In Human Prostate Cancer And Suppresses The Invasive Phenotype Of Prostate Cancer Cells., Matteo Fassan, Domenico D'Arca, Juraj Letko, Andrea Vecchione, Marina P Gardiman, Peter Mccue, Bernadette Wildemore, Massimo Rugge, Dolores Shupp-Byrne, Leonard G Gomella, Andrea Morrione, Renato V Iozzo, Raffaele Baffa

Kimmel Cancer Center Faculty Papers

MITOSTATIN, a novel putative tumor suppressor gene induced by decorin overexpression, is expressed in most normal human tissues but is markedly down-regulated in advanced stages of mammary and bladder carcinomas. Mitostatin negatively affects cell growth, induces cell death and regulates the expression and activation levels of Hsp27. In this study, we demonstrated that ectopic expression of Mitostatin in PC3, DU145, and LNCaP prostate cancer cells not only induced a significant reduction in cell growth, but also inhibited migration and invasion. Moreover, Mitostatin inhibited colony formation in soft-agar of PC3 and LNCaP cells as well as tumorigenicity of LNCaP cells in …

L-Arginine-Glycine Amidinotransferase, Betaine-Homocysteine S-Methyltransferase, And Neuropolypeptide H3 Are Diminished In Renal Clear Cell Carcinoma Of Humans., Jian-Ping Peng, Jie Zhang, Hsuan-Wei Huang, Feng-Hua Wang, Xian-Jin Du, Peng-Cheng Luo

Saudi Medical Journal

OBJECTIVES: To identify renal clear cell carcinoma-associated marker proteins. METHODS: Twelve patients with renal cell carcinoma (RCC) were collected and processed in the Department of Urology, Renmin Hospital, Wuhan University, China, between January 2008 and September 2009. Two-dimensional polyacrylamide gel electrophoresis and matrix assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) were employed to investigate differentially expressed protein spots between RCC tissues and adjacent normal tissues, then reverse transcription polymerase chain reaction and western blot were employed to confirm the proteomic results. RESULTS: One protein spot was upregulated, 13 were downregulated, and 22 were absent in RCC tissues. Four of …

In Vitro Antimicrobial Activity Of Propolis, Biopure Mtad, Sodium Hypochlorite, And Chlorhexidine On Enterococcus Faecalis And Candida Albicans., Soley Arslan, Hatice Ozbilge, Esma G. Kaya, Ozgur Er

Saudi Medical Journal

OBJECTIVES: To evaluate the antimicrobial effect by measuring the minimum inhibitory concentration MIC and minimum bactericidal concentration MBC of propolis, BioPure MTAD, 5% sodium hypochlorite (NaOCl), and 2% chlorhexidine CHX on Enterococcus faecalis (E. faecalis) and Candida albicans (C. albicans) in vitro. METHODS: This study was performed in the Faculty of Dentistry and Pharmacy at Erciyes University, Kayseri, Turkey from February to April 2010. Ethanol extract of propolis (EEP) was prepared from propolis collected from Kayseri, Turkey, and proper media for microorganisms were prepared using sterile broth medium to give final concentrations between 0.002-2.4 mg/ml for propolis, 0.000125-0.512 mg/ml for …

Drug Susceptibility Pattern Of Mycobacterium Tuberculosis Isolates Against Conventional Anti-Tuberculosis Drugs In Dhaka, Bangladesh., Abdul Mottalib, Murad Hossain, Ibrahim Khalil, Sirazul Islam, Anwar Hossain

Saudi Medical Journal

OBJECTIVES: To investigate the drug susceptibility pattern of isolated Mycobacterium tuberculosis (M. tuberculosis) against conventional anti-tuberculosis drugs in Dhaka, Bangladesh. METHODS: Sputum samples from 101 suspected new and previously treated patients were collected and M. tuberculosis was identified by microscopic observation and Ziehl-Neelsen staining. Drug susceptibility was performed against 4 anti-tuberculosis drugs, and the obtained data was analyzed. This study was performed in the Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders Hospital, Dhaka, Bangladesh between October 2008 and November 2009. RESULTS: Among 101 suspected, 59 (58.4%) cases were identified as M. tuberculosis and the drug …

Extremely Elevated Erythrocyte Sedimentation Rate., Mahmood D. Al-Mendalawi

Saudi Medical Journal

No abstract provided.

Applying Grounded Theory To Weight Management Among Women: Making A Commitment To Healthy Eating, Christie Zunker, Nataliya Ivankova

The Qualitative Report

In this study we developed a theory grounded in data from women who continued healthy eating behaviors after a weight management program. Participant recruitment was guided by theoretical sampling strategies for focus groups and individual interviews. Inclusion criteria were: African American or Caucasian women aged 30+ who lost > 5% of their body weight in a weight management program > year ago. Participants > 5% below their baseline weight were maintainers (n = 9); those above were non-maintainers (n = 14). We asked open-ended questions regarding healthy eating behaviors. The systematic design is described in detail, including categories from open coding, connection during …

Characterization Of Drug Interactions With Serum Proteins By Using High-Performance Affinity Chromatography, David S. Hage, Jeanethe Anguizola, Omar S. Barnaby, Abby Jo Jackson, Michelle J. Yoo, Efthimia Papastavros, Erika Pfaunmiller, Matt Sobansky, Zenghan Tong

David Hage Publications

The binding of drugs with serum proteins can affect the activity, distribution, rate of excretion, and toxicity of pharmaceutical agents in the body. One tool that can be used to quickly analyze and characterize these interactions is high-performance affinity chromatography (HPAC). This review shows how HPAC can be used to study drug-protein binding and describes the various applications of this approach when examining drug interactions with serum proteins. Methods for determining binding constants, characterizing binding sites, examining drug-drug interactions, and studying drug-protein dissociation rates will be discussed. Applications that illustrate the use of HPAC with serum binding agents such as …

The Effect Of The Chemical Composition Of Low Salinity Water On The Growth, Development, And Survival Of Cultured Juvenile Blue Crabs, Dyan Patricia Gibson

Master's Theses

Cultured juvenile blue crabs ( Callinectes sapidus Rathbun) were held for up to 77 days and monitored for survival, growth and development in low salinity water containing various compositions of ions. ~rabs were isolated in separate containers and placed in thirty gallon aquaria containing 2ppt of ion solution. Temperature, ambient, humidity, and nitrite levels were monitored daily. Molts were measured to determine growth, and molt increments were recorded to determine rate of development. The first part of the study compared ratios of sodium chloride (NaCl) to commercial sea salt (SS) in five treatments: 100% NaCl, 100% SS, 25:75 NaCl/SS, 50:50 …

Rickettsia Parkeri In Gulf Coast Ticks, Southeastern Virginia, Usa, Chelsea L. Wright, Robyn M. Nadolny, Ju Jiang, Allen L. Richards, Daniel E. Sonenshine, Holly D. Gaff, Wayne L. Hynes

Biological Sciences Faculty Publications

We report evidence that Amblyomma maculatum tick populations are well established in southeastern Virginia. We found that 43.1% of the adult Gulf Coast ticks collected in the summer of 2010 carried Rickettsia parkeri, suggesting that persons living in or visiting southeastern Virginia are at risk for infection with this pathogen.

Effects Of Gypsy Moth Outbreaks On North American Woodpeckers, Walter D. Koenig, Eric L. Walters, Andrew M. Liebhold

Biological Sciences Faculty Publications

We examined the effects of the introduced gypsy moth (Lymantria dispar) on seven species of North American woodpeckers by matching spatially explicit data on gypsy moth outbreaks with data on breeding and wintering populations. In general, we detected modest effects during outbreaks: during the breeding season one species, the Red-headed Woodpecker (Melanerpes erythrocephalus), increased over pre-outbreak levels, while during the winter one species, the Yellow-bellied Sapsucker (Sphyrapicus varius), increased and one, the Downy Woodpecker (Picoides pubescens), decreased from pre-outbreak levels. Responses following outbreaks were similarly variable, and in general we were unsuccessful …

Reframing Suicide In The Military, George R. Mastroianni, Wilber J. Scott

The US Army War College Quarterly: Parameters

No abstract provided.

Mechanisms Of Non-Enzymatic Peptide Bond Formation: Thioester And Transamidation Routes, Danielle Amie Landry

Master's Theses

The reaction mechanisms employed during non-ribosomal peptide (amide) synthesis in the non-ribosomal modular enzyme complex and in the enzyme glutaminyl cyclase were also used for spontaneous peptide (amide) synthesis in a buffered, abiotic solution. Analytical analyses revealed that spontaneous peptide (amide) bond formation occurred under mild prebiotic conditions during both the thioester-based production of phenylalanine peptides and peptidyl thioesters, and the spontaneous cyclization of glutamine t-butyl ester (Gln-tbe) into pyroglutamic acid t-butyl ester. Internal standard-based quantitative MS determined that 4.9 x 10-5 sec-1 was the rate constant for spontaneous Gln-tbe cyclization in imidazole buffer, pH 7, at 50°C, and that …

Investigating Mechanisms Of Co-Occurrence Between Two Similar Specis Of Predaceous Diving Beetles (Coleoptera: Dytiscidae) In Temporary Aquatic Habitats, Kristopher Alexander Pitcher

Master's Theses

Predaceous diving beetles (Coleoptera: Dytiscidae) are a model group of organisms for testing coexistence and exclusion mechanisms because numerous species of the family interact in isolated, aquatic habitats, and are ubiquitous throughout landscapes. Two morphologically similar species, laccophilus fasciatus rufus and Laccophilus proximus, co-occur in numerous habitats in South Mississippi, but are hypothesized to have strong interspecific interactions. I investigated several possible mechanisms that may promote such coexistence, including segregation in habitat domain, differences in behavior, and differences in dispersal that may allow co-occurrence between these two species of beetle. Habitat domain and behaviors such as activity and prey consumption …

Discovery And Molecular Characterization Of Selenoprotein M In The Salivary Glands Of Amblyomma Maculatum, The Gulf Coast Tick, Parul Singh

Master's Theses

The Gulfcoast ticks transmit disease-causing pathogens to humans and animals. Rickettsia parkeri is notable among the pathogens transmitted by A. maculatum to humans. Heavy infestations of A. maculatum on animal ears cause them to become thickened and curled, a condition commonly called "gotch ear." The tick's multifunctional salivary glands are vital to their biological success and likely also play a critical role in transmission of disease; tick saliva contains a broad array of secretory products that facilitate prolonged tick attachment and feeding; disrupting tick blood feeding or inactivating key tick salivary proteins presents a novel strategy for tick-borne disease prevention. …