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2011

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Factors Controlling Dissolved Organic Carbon Lability And Ecological Fate In The East Branch Swift River, Massachusettes, Hall Eric Dec 2011

Factors Controlling Dissolved Organic Carbon Lability And Ecological Fate In The East Branch Swift River, Massachusettes, Hall Eric

Theses and Dissertations

Fluvial systems have been estimated to transform, transport, or store 2.75 petagrams (Pg) of Organic Carbon (OC) per year. Although approximately 1Pg per year of terrestrial carbon is fluxed to the atmosphere through inland waters, little is known about the factors regulating its eventual ecological fate. 28 day lability incubations were conducted concurrent with the measurement of several environmental parameters including discharge, nutrient concentration, DO13C, and DOC:DON at several sites along Bigelow Brook and the East Branch of the Swift River, Massachusetts. Temporal and spatial variation of DOC, DOC:DON and DO13C were explored. Two distinct DOC consumption rates, short and …


Global Change, Global Trade, And The Next Wave Of Plant Invasions, Bethany A. Bradley, Dana M. Blumenthal, Regan Early, Edwin D. Grosholz, Joshua J. Lawler, Luke P. Miller, Cascade J.B. Sorte, Carla M. D'Antonio, Jeffrey M. Diez, Jeffrey S. Dukes, Ines Ibanez, Julian D. Olden Dec 2011

Global Change, Global Trade, And The Next Wave Of Plant Invasions, Bethany A. Bradley, Dana M. Blumenthal, Regan Early, Edwin D. Grosholz, Joshua J. Lawler, Luke P. Miller, Cascade J.B. Sorte, Carla M. D'Antonio, Jeffrey M. Diez, Jeffrey S. Dukes, Ines Ibanez, Julian D. Olden

Faculty Publications, Biological Sciences

Many non-native plants in the US have become problematic invaders of native and managed ecosystems, but a new generation of invasive species may be at our doorstep. Here, we review trends in the horticultural trade and invasion patterns of previously introduced species and show that novel species introductions from emerging horticultural trade partners are likely to rapidly increase invasion risk. At the same time, climate change and water restrictions are increasing demand for new types of species adapted to warm and dry environments. This confluence of forces could expose the US to a range of new invasive species, including many …


Montana Kaimin, December 2, 2011, Students Of The University Of Montana, Missoula Dec 2011

Montana Kaimin, December 2, 2011, Students Of The University Of Montana, Missoula

Montana Kaimin, 1898-present

Student newspaper of the University of Montana, Missoula.


Pulmonary Diffusional Screening And The Scaling Laws Of Mammalian Metabolic Rates, Chen Hou, Michael Mayo Dec 2011

Pulmonary Diffusional Screening And The Scaling Laws Of Mammalian Metabolic Rates, Chen Hou, Michael Mayo

Biological Sciences Faculty Research & Creative Works

Theoretical considerations suggest that the mammalian metabolic rate is linearly proportional to the surface areas of mitochondria, capillary, and alveolar membranes. However, the scaling exponents of these surface areas to the mammals' body mass (approximately 0.9-1) are higher than exponents of the resting metabolic rate (RMR) to body mass (approximately 0.75), although similar to the one of exercise metabolic rate (EMR); the underlying physiological cause of this mismatch remains unclear. The analysis presented here shows that discrepancies between the scaling exponents of RMR and the relevant surface areas may originate from, at least for the system of alveolar membranes in …


Spatial, Ontogenetic, And Sexual Effects On The Diet Of A Teiid Lizard In Arid South America, J Van Leeuwen, Alessandro Catenazzi, M Holmgren Dec 2011

Spatial, Ontogenetic, And Sexual Effects On The Diet Of A Teiid Lizard In Arid South America, J Van Leeuwen, Alessandro Catenazzi, M Holmgren

Publications

Most lizard species feed on small arthropods, and although some are omnivorous, only a few species are strict herbivores. We studied the diet of Dicrodon guttulatum, a teiid lizard endemic to the arid coastal deserts and dry forests of northern Peru. Herbivory by this lizard has been identified as a potential limiting factor in the regeneration of plant communities in these dry ecosystems. We collected gastric and fecal samples of adult males, adult females, and juveniles of D. guttulatum in different plant communities. Based on the ontogenetic shift from insectivory to herbivory observed in other herbivorous lizards, we hypothesized …


Using Prolonged Exposure To Treat Abortion-Related Posttraumatic Stress Disorder In Alcohol-Dependent Men: A Case Study, Aisha Denise Baker, Jay A. Morrison, Scott Coffey Dec 2011

Using Prolonged Exposure To Treat Abortion-Related Posttraumatic Stress Disorder In Alcohol-Dependent Men: A Case Study, Aisha Denise Baker, Jay A. Morrison, Scott Coffey

Student Publications

Men’s reactions to a partner’s abortion are an understudied area. Few studies have examined abortion as it relates to posttraumatic stress disorder (PTSD) in males, and no studies have examined the use of an empirically supported behavioral treatment for PTSD in this population. The current case study examines prolonged exposure (PE) for the treatment of abortion-related PTSD in a 46-year-old White male who also has alcohol dependence. The patient was involved in a residential substance abuse treatment program at the time of treatment. After receiving 12 sessions of PE, the patient experienced a decrease in PTSD symptoms as measured by …


Quantifying Burned Area For North American Forests: Implications For Direct Reduction Of Carbon Stocks, Eric S. Kasischke, Tatiana Loboda, Louis Giglio, Nancy H. F. French, E. E. Hoy, Bernardus De Jong, David Riano Dec 2011

Quantifying Burned Area For North American Forests: Implications For Direct Reduction Of Carbon Stocks, Eric S. Kasischke, Tatiana Loboda, Louis Giglio, Nancy H. F. French, E. E. Hoy, Bernardus De Jong, David Riano

Michigan Tech Research Institute Publications

A synthesis was carried out to analyze information available to quantify fire activity and burned area across North America, including a comparison of different data sources and an assessment of how variations in burned area estimate impact carbon emissions from fires. Data sets maintained by fire management agencies provide the longest record of burned area information. Canada and Alaska have the most well developed data sets consisting of the perimeters of large fires (>200 ha) going back to 1959 and 1950, respectively. A similar data set back to 1980 exists for the Conterminous U.S., but contains data only from …


Model Comparisons For Estimating Carbon Emissions From North American Wildland Fire, Nancy H. F. French, Willam J. De Groot, Liza K. Jenkins, Brendan M. Rogers, Ernesto Alvarado, Brian Amiro, Bernardus De Jong, Scott Goetz, Elizabeth Hoy, Edward Hyer, Robert Keane, B. E. Law, Donald Mckenzie, Steven G. Mcnulty, Roger Ottmar, Diego R. Perez-Salicrup, James Randerson, Kevin M. Robertson, Merritt Turetsky Dec 2011

Model Comparisons For Estimating Carbon Emissions From North American Wildland Fire, Nancy H. F. French, Willam J. De Groot, Liza K. Jenkins, Brendan M. Rogers, Ernesto Alvarado, Brian Amiro, Bernardus De Jong, Scott Goetz, Elizabeth Hoy, Edward Hyer, Robert Keane, B. E. Law, Donald Mckenzie, Steven G. Mcnulty, Roger Ottmar, Diego R. Perez-Salicrup, James Randerson, Kevin M. Robertson, Merritt Turetsky

Michigan Tech Research Institute Publications

Research activities focused on estimating the direct emissions of carbon from wildland fires across North America are reviewed as part of the North American Carbon Program disturbance synthesis. A comparison of methods to estimate the loss of carbon from the terrestrial biosphere to the atmosphere from wildland fires is presented. Published studies on emissions from recent and historic time periods and five specific cases are summarized, and new emissions estimates are made using contemporary methods for a set of specific fire events. Results from as many as six terrestrial models are compared. We find that methods generally produce similar results …


Vulnerability Of High Latitude Soil Organic Carbon In North America To Disturbance, Guido Grosse, Jennifer Harden, Merritt Turetsky, David A. Mcguire, Philip Camill, Charles Tarnocai, Steve Frolking, Edward A.G. Schuur, Torre Jorgenson, Sergei Marchenko, Vladimir Romanovsky, Kimberly P. Wickland, Nancy H. F. French, Mark Waldrop, Laura Bourgeau-Chavez, Robert G. Striegl Dec 2011

Vulnerability Of High Latitude Soil Organic Carbon In North America To Disturbance, Guido Grosse, Jennifer Harden, Merritt Turetsky, David A. Mcguire, Philip Camill, Charles Tarnocai, Steve Frolking, Edward A.G. Schuur, Torre Jorgenson, Sergei Marchenko, Vladimir Romanovsky, Kimberly P. Wickland, Nancy H. F. French, Mark Waldrop, Laura Bourgeau-Chavez, Robert G. Striegl

Michigan Tech Research Institute Publications

This synthesis addresses the vulnerability of the North American high-latitude soil organic carbon (SOC) pool to climate change. Disturbances caused by climate warming in arctic, subarctic, and boreal environments can result in significant redistribution of C among major reservoirs with potential global impacts. We divide the current northern high-latitude SOC pools into (1) near-surface soils where SOC is affected by seasonal freeze-thaw processes and changes in moisture status, and (2) deeper permafrost and peatland strata down to several tens of meters depth where SOC is usually not affected by short-term changes. We address key factors (permafrost, vegetation, hydrology, paleoenvironmental history) …


Biological Sex As A Predictor Of Competitive Success In Intercollegiate Forensics, Kiranjeet Dhillon, April Larson Dec 2011

Biological Sex As A Predictor Of Competitive Success In Intercollegiate Forensics, Kiranjeet Dhillon, April Larson

National Forensic Journal

This study examines biological sex as a predictor of the level of success in intercollegiate policy debate, impromptu speaking, and extemporaneous speaking. Secondary data analysis of tabulation sheets from NDT, AFA-NIET, and NFA, revealed three findings. First, there are more male than female competitors in policy debate and males significantly experienced more out-round success than females. Second, there are more males than females in impromptu speaking; however, there was no significance between biological sex and success in out-rounds. Third, there are more male than female competitors in extemporaneous speaking and males significantly experienced more out-round success than females.


The Economics Of Partial Artemia Replacement Using Two Commercially Available Feeds In The Diets Of Litopenaeus Vannamei From Z3/M1 – Pl10, Monique A. Giguere Dec 2011

The Economics Of Partial Artemia Replacement Using Two Commercially Available Feeds In The Diets Of Litopenaeus Vannamei From Z3/M1 – Pl10, Monique A. Giguere

Open Access Theses

The purpose of this study was twofold: 1) to identify commercially available feeds that could serve as suitable replacements for newly hatched Artemia in the diets of L. vannamei from Z3/M1 to PL10 without significantly affecting survival, final length and weight, and quality of the larvae and 2) to identify an ideal substitution rate between live Artemia and a replacement feed that maximizes feed and labor costs savings, survival, and PL quality. In Experiment 1, two commercially available Artemia replacement feeds, Zeigler EZ Artemia and Bernaqua Vitellus, were administered according to manufacturer’s guidelines in order to identify which feed served …


Modularity, Theory Of Mind, And Autism Spectrum Disorder, Marcus P. Adams Dec 2011

Modularity, Theory Of Mind, And Autism Spectrum Disorder, Marcus P. Adams

Philosophy Faculty Scholarship

The theory of mind (ToM) deficit associated with autism spectrum disorder has been a central topic in the debate about the modularity of the mind. In a series of papers, Philip Gerrans and Valerie Stone argue that positing a ToM module does not best explain the deficits exhibited by individuals with autism. In this article, I first criticize Gerrans and Stone’s account. Second, I discuss various studies of individuals with autism and argue that they are best explained by positing a higher-level, domain-specific ToM module.


Polymorphic Variation Of The Dgat1 Gene In Cal Poly Holstein And Jersey Breeds And The Correlation Of Milk Fat Content, And Genetic Values, Andrea M. Laubscher Dec 2011

Polymorphic Variation Of The Dgat1 Gene In Cal Poly Holstein And Jersey Breeds And The Correlation Of Milk Fat Content, And Genetic Values, Andrea M. Laubscher

Master's Theses

The selection of dairy cattle is dictated by improvement of yield and composition of milk. Genotyping tests give breeders the opportunity to use previously unavailable information to increase the frequency of beneficial alleles in their herds. A QTL influencing milk yield and composition is located in the centromeric end of chromosome 14. The polymorphism being analyzed is a K232A substitution (lysine to alanine amino acid change) in exon VII of the diacylglycerol acyl transferase 1 (DGAT1) gene. DGAT1 is a key acyltranferase in triglyceride biosynthesis. DGAT1 encodes an enzyme which catalyzes the final step of mammalian triglyceride synthesis from carbohydrates. …


Case 279 Proposed Order, Joe L. Allen Dec 2011

Case 279 Proposed Order, Joe L. Allen

Hedden-Nicely Collection, All

Case 279 involves several claims, all involving the Sycan River, involving tribal claims for instream water rights. (Proposed Order)


Case 279 Proposed Order, Joe L. Allen Dec 2011

Case 279 Proposed Order, Joe L. Allen

In re Klamath River (Klamath Tribe)

Case 279 involves several claims, all involving the Sycan River, involving tribal claims for instream water rights. (Proposed Order)


Mole Street Journal, November 2011, University Of Arkansas, Fayetteville. Dept. Of Chemistry And Biochemistry Dec 2011

Mole Street Journal, November 2011, University Of Arkansas, Fayetteville. Dept. Of Chemistry And Biochemistry

Mole Street Journal

No abstract provided.


Neuronal Growth Cone Retraction Relies Upon Proneurotrophin Receptor Signaling Through Rac, Richard E. Mains, Betty A. Eipper Dec 2011

Neuronal Growth Cone Retraction Relies Upon Proneurotrophin Receptor Signaling Through Rac, Richard E. Mains, Betty A. Eipper

UCHC Articles - Research

Growth of axons and dendrites is a dynamic process that involves guidance molecules, adhesion proteins, and neurotrophic factors. Although neurite extension during development has been extensively studied, the intracellular mechanisms that mediate neurite retraction are poorly understood. Here, we show that the proneurotrophin, proNGF, induces acute collapse of growth cones of cultured hippocampal neurons. This retraction is initiated by an interaction between p75NTR and the sortilin family member, SorCS2 (sortilin-related VPS10 domain containing receptor 2). Binding of proNGF to the p75NTR-SorCS2 complex induced growth cone retraction by initiating the dissociation of the guanine-nucleotide exchange factor Trio from …


What Are The Effects Of Raw Vs. Pasteurized Milk Consumption On Growth Rate And Fertility In A Colony Of Mice?, Kari Sholing, Lindsay Ganong, Amy Olson Dec 2011

What Are The Effects Of Raw Vs. Pasteurized Milk Consumption On Growth Rate And Fertility In A Colony Of Mice?, Kari Sholing, Lindsay Ganong, Amy Olson

Nutrition Student Work

Interest in consuming unprocessed foods has led to assumptions that raw milk has health benefits over pasteurized milk. This study was designed to evaluate the belief that raw milk is nutritionally superior. Eighteen mice were randomly assigned to one of two groups: raw milk or pasteurized milk. Mice were grouped into breeding trios and given a fresh supply of milk every four hours between 7 a.m. and 11 p.m. for 55 days. Milk consumption was tracked at each feeding by measuring the amount of milk that was provided and the amount of milk that was left from the previous feeding. …


Primetimes Newsletter, Winter 2011, Office Of Lifespan Studies Dec 2011

Primetimes Newsletter, Winter 2011, Office Of Lifespan Studies

PrimeTimes Newsletter

PrimeTimes is the newsletter of the Office of Lifespan Studies in the College of Science at Coastal Carolina University.


Adaptive Management Of Bull Trout Populations In The Lemhi Basin, Andrew J. Tyre, James T. Peterson, Sarah J. Converse, Tiffany Bogich, Damien Miller, Max Post Van Der Burg, Carmen Thomas, Ralph Thompson, Jeri Wood, Donna C. Brewer, Michael C. Runge Dec 2011

Adaptive Management Of Bull Trout Populations In The Lemhi Basin, Andrew J. Tyre, James T. Peterson, Sarah J. Converse, Tiffany Bogich, Damien Miller, Max Post Van Der Burg, Carmen Thomas, Ralph Thompson, Jeri Wood, Donna C. Brewer, Michael C. Runge

School of Natural Resources: Faculty Publications

The bull trout Salvelinus confluentus, a stream-living salmonid distributed in drainages of the northwestern United States, is listed as threatened under the Endangered Species Act because of rangewide declines. One proposed recovery action is the reconnection of tributaries in the Lemhi Basin. Past water use policies in this core area disconnected headwater spawning sites from downstream habitat and have led to the loss of migratory life history forms. We developed an adaptive management framework to analyze which types of streams should be prioritized for reconnection under a proposed Habitat Conservation Plan. We developed a Stochastic Dynamic Program that identified optimal …


Monascus Pigment Production By Solid-State Fermentation With Corn Cob Substrate, Palanivel Velmurugan, Hyun Hur, Vellingiri Balachandar, Seralathan Kamala-Kannan, Kui-Jae Lee, Sang-Myung Lee, Jong-Chan Chae, Patrick Shea, Byung-Taek Oh Dec 2011

Monascus Pigment Production By Solid-State Fermentation With Corn Cob Substrate, Palanivel Velmurugan, Hyun Hur, Vellingiri Balachandar, Seralathan Kamala-Kannan, Kui-Jae Lee, Sang-Myung Lee, Jong-Chan Chae, Patrick Shea, Byung-Taek Oh

School of Natural Resources: Faculty Publications

Natural pigments are an important alternative to potentially harmful synthetic dyes. We investigated the feasibility of corn cob powder as a substrate for production of pigments by Monascus purpureus KACC 42430 in solid-state fermentation. A pigment yield of 25.42 OD Units/gram of dry fermented substrate was achieved with corn cob powder and optimized process parameters, including 60% (w/w) initial moisture content, incubation at 30°C, inoculation with 4 mL of spores/gram of dry substrate, and an incubation period of 7 days. Pigment yield using corn cobs greatly exceeded those of most other agricultural waste substrates. The pigments were stable at acidic …


Characterizing The Performance Of Ecosystem Models Across Time Scales: A Spectral Analysis Of The North American Carbon Program Site-Level Synthesis, Michael C. Dietze, Rodrigo Vargas, Andrew D. Richardson, Paul C. Stoy, Alan G. Barr, Ryan S. Anderson, M. Altaf Arain, Ian T. Baker, T. Andrew Black, Jing M. Chen, Philippe Ciais, Lawrence B. Flanagan, Christopher M. Gough, Robert F. Grant, David Hollinger, R. Cesar Izaurralde, Christopher J. Kucharik, Peter Lafleur, Shugang Liu, Erandathie Lokupitiya, Yiqi Luo, J. William Munger, Changhui Peng, Benjamin Poulter, David T. Price, Daniel M. Ricciuto, William J. Riley, Alok Kumar Sahoo, Kevin Schaefer, Andrew E. Suyker, Hanqin Tian, Christina Tonitto, Hans Verbeeck, Shashi B. Verma, Weifeng Wang, Ensheng Weng Dec 2011

Characterizing The Performance Of Ecosystem Models Across Time Scales: A Spectral Analysis Of The North American Carbon Program Site-Level Synthesis, Michael C. Dietze, Rodrigo Vargas, Andrew D. Richardson, Paul C. Stoy, Alan G. Barr, Ryan S. Anderson, M. Altaf Arain, Ian T. Baker, T. Andrew Black, Jing M. Chen, Philippe Ciais, Lawrence B. Flanagan, Christopher M. Gough, Robert F. Grant, David Hollinger, R. Cesar Izaurralde, Christopher J. Kucharik, Peter Lafleur, Shugang Liu, Erandathie Lokupitiya, Yiqi Luo, J. William Munger, Changhui Peng, Benjamin Poulter, David T. Price, Daniel M. Ricciuto, William J. Riley, Alok Kumar Sahoo, Kevin Schaefer, Andrew E. Suyker, Hanqin Tian, Christina Tonitto, Hans Verbeeck, Shashi B. Verma, Weifeng Wang, Ensheng Weng

School of Natural Resources: Faculty Publications

Ecosystem models are important tools for diagnosing the carbon cycle and projecting its behavior across space and time. Despite the fact that ecosystems respond to drivers at multiple time scales, most assessments of model performance do not discriminate different time scales. Spectral methods, such as wavelet analyses, present an alternative approach that enables the identification of the dominant time scales contributing to model performance in the frequency domain. In this study we used wavelet analyses to synthesize the performance of 21 ecosystem models at 9 eddy covariance towers as part of the North American Carbon Program’s site-level intercomparison. This study …


Heritable Choice Of Colony Size In Cliff Swallows: Does Experience Trump Genetics In Older Birds?, Erin A. Roche, Charles R. Brown, Mary Bomberger Brown Dec 2011

Heritable Choice Of Colony Size In Cliff Swallows: Does Experience Trump Genetics In Older Birds?, Erin A. Roche, Charles R. Brown, Mary Bomberger Brown

School of Natural Resources: Faculty Publications

The variation in breeding colony size seen in populations of most colonial birds may reflect heritable choices made by individuals that are phenotypically specialized for particular social environments. Although a few studies have reported evidence for genetically based choice of group sizes in birds, we know relatively little about the extent to which animals potentially rely on experience versus innate preferences in deciding how many conspecifics to settle with at different times of their lives. We conducted a cross-fostering experiment in 1997–1998 on cliff swallows, Petrochelidon pyrrhonota, in southwestern Nebraska, USA, in which some individuals were reared in colonies …


Characterization Of An S-Layer-Like Protein And Select Antimicrobial Factors Of The Novel Species Lactobacillus Coleohominis, Nicole Ann Marie Ayala Dec 2011

Characterization Of An S-Layer-Like Protein And Select Antimicrobial Factors Of The Novel Species Lactobacillus Coleohominis, Nicole Ann Marie Ayala

Theses & Dissertations

Lactobacilli in the vaginal tract have been reported be protective against infections with vaginal pathogenic microorganisms through the production of antimicrobial factors, competition for adherence to vaginal epithelium, and coaggregation with pathogens to aid in their clearing. In addition, the antimicrobial factors produced by certain species of lactobacilli have even been shown to interrupt the replication or alter the function of select viruses. Vaginal lactobacilli have been investigated for their antimicrobial characteristics, but little is known about the novel species L coleohominis. recently described in vaginal isolates (Nikolaitchouk et al. 2001). It was hypothesized that L coleohominis, found …


No True Men In The State : Pseudo/Translation And "Feminine" Voice In The Late-Qing = 國無人乎? : 晚清的偽/翻譯和"女性"話語, Michael Gibbs Hill Dec 2011

No True Men In The State : Pseudo/Translation And "Feminine" Voice In The Late-Qing = 國無人乎? : 晚清的偽/翻譯和"女性"話語, Michael Gibbs Hill

Journal of Modern Literature in Chinese 現代中文文學學報

No abstract provided.


Organ-Distribution Of The Metabolite 2-Aminothiazoline-4-Carboxylic Acid In A Rat Model Following Cyanide Exposure, Ilona Petrikovics, David E. Thompson, Gary A. Rockwood, Brian A. Logue Dec 2011

Organ-Distribution Of The Metabolite 2-Aminothiazoline-4-Carboxylic Acid In A Rat Model Following Cyanide Exposure, Ilona Petrikovics, David E. Thompson, Gary A. Rockwood, Brian A. Logue

Chemistry and Biochemistry Faculty Publications

The reaction of cyanide (CN−) with cystine to produce 2-aminothiazoline-4-carboxylic acid (ATCA) is one of the independent detoxification pathways of cyanide in biological systems. In this report, in vivo production of ATCA and its distributions in plasma and organs were studied after a subcutaneous sublethal dose of 4 mg/kg body weight potassium cyanide (KCN) administration to rats. At this sublethal dose of KCN, ATCA concentration was not significantly increased in the plasma samples, however, it was found significantly increased in liver samples. These results suggested that ATCA might not be a good diagnostic biomarker in plasma for sublethal …


Infection Connection, Lehigh Valley Health Network Dec 2011

Infection Connection, Lehigh Valley Health Network

Infection Connection

No abstract provided.


Progress Notes, Lehigh Valley Health Network Dec 2011

Progress Notes, Lehigh Valley Health Network

Progress Notes

No abstract provided.


The Lab Report, Volume 05, Issue 07, Todd Ristau Dec 2011

The Lab Report, Volume 05, Issue 07, Todd Ristau

Lab Reports

  • Tesseract Theatre Calls for Submissions
  • Student Spotlight: Taylor Gruenloh
  • Guest Profile: Gina Kaufmann
  • Last Hope for Twenty Miles by Ricky Steven Young
  • War Bride Read at SkyPilot
  • No Shame Central Coast Celebrates First Anniversary
  • Fast N' Loose #40 Includes Hollins Playwright
  • Mary’s Dream by Yulia Gray
  • 12 Plays of Christmas at SkyPilot
  • The Perfect Gift by Wendy-Marie Foerster at Secret Rose Theatre
  • Clarissa's Cat by Darlene Fedele at James River Conference Center
  • After Hours at the Dead Legend at University of Great Falls
  • Consider a Year End Gift to the New Works Initiative
  • From the Director: What's in Your Manifesto?


Citrate Binding To The Membrane Protein Proteorhodopsin, Farhana F. Syed Dec 2011

Citrate Binding To The Membrane Protein Proteorhodopsin, Farhana F. Syed

Chemistry - Dissertations

Proteorhodopsin (pR) is an intrinsic membrane protein with an important role in solar-energy storage of the biosphere. Earlier work in our lab has shown that polyhistidine-tagged pR can be purified by means of selective precipitation with citrate under specific conditions, as can a number of mutants based on this His6-tagged pR. Purification of a heterologously-expressed trans-membrane protein by a simple salt such as citrate is novel. However, such a phenomenon leads to several questions: How does citrate cause pR precipitation? Does the polyhistidine-tag assist in such a precipitation? Is this precipitation pH-specific? Does citrate affect the function of …