Digital Commons Network^™

Species D Adenoviruses As Oncolytics Against B-Cell Cancers, Christopher Y. Chen, Julien S. Senac, Eric A. Weaver, Shannon M. May, Diane F. Jelinek, Philip Greipp, Thomas Witzig, Michael A. Barry

Nebraska Center for Virology: Faculty Publications

Purpose: Oncolytic viruses are self-amplifying anticancer agents that make use of the natural ability of viruses to kill cells. Adenovirus serotype 5 (Ad5) has been extensively tested against solid cancers, but less so against B-cell cancers because these cells do not generally express the coxsackie and adenoviral receptor (CAR). To determine whether other adenoviruses might have better potency, we "mined" the adenovirus virome of 55 serotypes for viruses that could kill B-cell cancers.

Experimental Design: Fifteen adenoviruses selected to represent Ad species B, C, D, E, and F were tested in vitro against cell lines and primary patient B-cell cancers …

Are Student Nurses Ready For Disasters? An Analysis Of Emergency Preparedness Content In Nursing School Textbooks Used In The Miami Valley, Kimberly Caudill

Master of Public Health Program Student Publications

Results of an analysis of nursing textbooks used in the Miami Valley, indicate that there is minimal emergency preparedness content included. The research was based on the textbooks used in the Introduction to Nursing, Psychiatric Nursing, and Community Health Nursing classes at four local nursing schools: Sinclair Community College, Kettering College of Medical Arts, Cedarville University, and Wright State University. The Community Health Nursing classes have the most prepared content, and include extra content in the form of tables and pictures .The analysis indicates that Wright State University does the best job at preparing student nurses to be ready to …

Islam And The Relation Of Science And The Qur'an, Mohd Yakub @ Zulkifli Mohd Yusoff, Majid Danehsgar

Research Publications (2011 to 2015)

The relation of science and religion is one of the most important interdisciplinary issues in the world from the past to the present. Any kinds of religion generally brought about a new challenging discussion with science; Islam and its scriptures are not exceptions in this case. The Qur'an as a main Islamic scripture has a direct relation with science and scientific matters according to many thinkers' declarations; it is obvious that there are opponents for this subject, on the other side. The purpose of this paper is to examine main categories of the relation of science and religion and then …

Problem Based Learning In Management And Clinical Engineering Course, Nur Azah Hamzaid, Mohd Faiz Md Saaid, Shuhaibul Fadzly Mansor, Azman Hamid

Research Publications (2011 to 2015)

This paper addressed student perceptions and opinion of the problem based learning method, as well as the empirically collected data on students' learning outcomes on a Biomedical Engineering course, i.e. Management and Clinical Engineering. Pure lecture delivery with absence of practical intervention was deemed insufficient to provide appropriate means to achieve the course objectives. Therefore, a Mock Company assignment was introduced in this course as a problem based learning application aimed to aid the achievement of the programme outcome while improving the attainment of the course objectives. The students were divided into groups to form individual mock company. Each mock …

A Brain-Inspired Model Of Hierarchical Planner, Budhitama Subagdja, Ah-Hwee Tan

Research Collection School Of Computing and Information Systems

Hierarchical planning is an approach of planning by composing and executing hierarchically arranged plans to solve some problems. Most symbolic-based hierarchical planners have been devised to allow the knowledge to be described expressively. However, a great challenge is to automatically seek and acquire new plans on the fly. This paper presents a novel neural-based model of hierarchical planning that can seek and acquired new plans on-line if the necessary knowledge are lacking. Inspired by findings in neuropsychology, plans can be inherently learnt, retrieved, and manipulated simultaneously rather than discretely processed like in most symbolic approaches. Using a multi-channel adaptive resonance …

Consistent Community Identification In Complex Networks, Haewoon Kwak, Young-Ho Eom, Yoonchan Choi, Hawoong Jeong

Research Collection School Of Computing and Information Systems

We have found that known community identification algorithms produce inconsistent communities when the node ordering changes at input. We use the pairwise membership probability and consistency to quantify the level of consistency across multiple runs of an algorithm. Based on these two metrics, we address the consistency problem without compromising the modularity. The key insight of the algorithm is to use pairwise membership probabilities as link weights. It offers a new tool in the study of community structures and their evolutions.

Profiles Of Teachers In Selected Curriculum Areas : Further Analyses Of The Staff In Australia’S Schools 2010 Survey, Paul R. Weldon, Glenn Rowley, Phillip Mckenzie

School and system improvement

This report was commissioned by DEEWR and uses data from the 2010 Staff in Australia’s Schools (SiAS) survey to analyse the profiles of the teachers teaching in five selected curriculum areas in primary schools and 12 areas in secondary schools and to compare the 2010 data with that obtained in the 2007 SiAS survey. The areas were selected to help inform policy initiatives as well as concerns about teacher shortages in those areas and other related workforce issues.

This I Believe: The Do-Over, Meredith Doench

English Faculty Publications

I believe in second chances. Even thirds. There’s nothing like the power of a sincere do-over.

As a junior and senior high student, school was never my forte. It wasn’t for lack of effort on my parents’ part—my mother had been a fourth grade teacher and my father, a doctor, worked hard to keep me in one of the best districts in our area. Still, I bucked most school activities. Study groups? No way. Extra-curriculars? Not unless my friends were doing it. Math club? Please!

My junior year I fell into an anxious depression so severe, I required hospitalization. All …

Distress Tolerance Moderates The Relationship Between Negative Affect Intensity With Borderline Personality Disorder Levels, Marina Bornovalova, Alexis Matusiewicz, Elizabeth Rojas

Psychology Faculty Publications

A number of studies have suggested that negative emotionality and negative affect intensity play key roles in the development and maintenance of borderline personality disorder (BPD). However, more recent research indicates that one's response to affective discomfort may be an even more important variable in the pathogenesis of BPD than either negative emotionality or negative affect intensity per se. As such, the current study aimed to empirically test the moderating role of 2 well-validated laboratory measures of the ability to tolerate psychological distress (distress tolerance) in the relationship of negative emotionality and negative affect intensity with BPD levels. Results provide …

Synthesis And Antiproliferative Activity Of Some Steroidal Lactams, Yanmin Huang, Jianguo Cui, Sijing Chen, Chunfang Gan, Aimin Zhou

Chemistry Faculty Publications

Using cholesterol as starting material, a series of 6-substituted-3-aza-A-homo-3-oxycholestanes and 6-substituted-4-aza-A-homo-3-oxycholestanes were synthesized by the oxidation, reduction, oximation, Beckman rearrangement and condensation reaction. These synthesized compounds displayed a distinct cytotoxicity against MGC 7901, HeLa and SMMC 7404 cancer cells. Our results revealed that the structures of functional groups at position-6 on the steroidal ring are crucial for the IC50 value of antiproliferative activities of these compounds and the cytotoxic activity against MGC 7901 and SMMC 7404 cells was not significantly different between 4-N-lactams and 3-N-lactams when its 6-substituted group was a carbonyl or a hydroximino, but all 3-N-lactams showed a …

Boron Stress Activates The General Amino Acid Control Mechanism And Inhibits Protein Synthesis, Irem Uluisik, Alaattin Kaya, Dmitri E. Fomenko, Huseyin C. Karakaya, Bradley A. Carlson, Vadim N. Gladyshev, Ahmet Koc

Department of Biochemistry: Faculty Publications

Boron is an essential micronutrient for plants, and it is beneficial for animals. However, at high concentrations boron is toxic to cells although the mechanism of this toxicity is not known. Atr1 has recently been identified as a boron efflux pump whose expression is upregulated in response to boron treatment. Here, we found that the expression of ATR1 is associated with expression of genes involved in amino acid biosynthesis. These mechanisms are strictly controlled by the transcription factor Gcn4 in response to boron treatment. Further analyses have shown that boron impaired protein synthesis by promoting phosphorylation of eIF2α in a …

Can The Professor Come Out And Play?—Scholarship, Teaching, And Theories Of Play, Bryan Adamson, Lisa Brodoff, Marilyn Berger, Anne Enquist, Paula Lustbader, John B. Mitchell

Seattle Journal for Social Justice

No abstract provided.

Investigating Combinatorial Approaches In Virtual Screening On Human Inducible 6-Phosphofructo-2-Kinase/Fructose-2,6-Bisphosphatase (Pfkfb3): A Case Study For Small Molecule Kinases, Robert B. Crochet, Michael C. Cavalier, Minsuh Seo, Jeong Do Kim, Young Sun Yim, Seung Jong Park, Yong Hwan Lee

Faculty Publications

Efforts toward improving the predictiveness in tier-based approaches to virtual screening (VS) have mainly focused on protein kinases. Despite their significance as drug targets, small molecule kinases have been rarely tested with these approaches. In this paper, we investigate the efficacy of a pharmacophore screening-combined structure-based docking approach on the human inducible 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, an emerging target for cancer chemotherapy. Six out of a total 1364 compounds from NCI's Diversity Set II were selected as true actives via throughput screening. Using a database constructed from these compounds, five programs were tested for structure-based docking (SBD) performance, the MOE of which showed …

Arabidopsis Pht1; 5 Plays An Integral Role In Phosphate Homeostasis, Aaron P. Smith, Vinay K. Nagarajan, Kashchandra G. Raghothama

Faculty Publications

The mobilization of inorganic phosphate (Pi) in planta is a complex process regulated by a number of developmental and environmental cues. Plants possess many Pi transporters that acquire Pi from the rhizosphere and translocate it throughout the plant. A few members of the high-affinity Pht1 family of Pi transporters have been functionally characterized and, for the most part, have been shown to be involved in Pi acquisition. We recently demonstrated that the Arabidopsis Pi transporter, Pht1;5, plays a key role in translocating Pi between tissues. Loss-of-function pht1;5 mutant seedlings accumulated more P in shoots relative to wild type but less …

Radical-Containing Particles Activate Dendritic Cells And Enhance Th17 Inflammation In A Mouse Model Of Asthma, Pingli Wang, Paul Thevenot, Jordy Saravia, Terry Ahlert, Stephania A. Cormier

Faculty Publications

We identified a previously unrecognized component of airborne particulate matter (PM) formed in combustion and thermal processes, namely, environmentally persistent free radicals (EPFRs). The pulmonary health effects of EPFRs are currently unknown. In the present study, we used a model EPFR-containing pollutant-particle system referred to as MCP230. We evaluated the effects of MCP230 on the phenotype and function of bone marrow - derived dendritic cells (BMDCs) in vitro and lung dendritic cells (DCs) in vivo, and the subsequent T-cell response. We also investigated the adjuvant role of MCP230 on airway inflammation in a mouse model of asthma. MCP230 decreased intracellular …

Horizontal Transmission Of Rickettsia Felis Between Cat Fleas, Ctenocephalides Felis, Supanee Hirunkanokpun, Chutima Thepparit, Lane D. Foil, Kevin R. Macaluso

Faculty Publications

Rickettsia felis is a rickettsial pathogen primarily associated with the cat flea, Ctenocephalides felis. Although laboratory studies have confirmed that R. felis is maintained by transstadial and transovarial transmission in C. felis, distinct mechanisms of horizontal transmission of R. felis among cat fleas are undefined. Based on the inefficient vertical transmission of R. felis by cat fleas and the detection of R. felis in a variety of haematophagous arthropods, we hypothesize that R. felis is horizontally transmitted between cat fleas. Towards testing this hypothesis, flea transmission of R. felis via a bloodmeal was assessed weekly for 4 weeks. Rhodamine B …

Draft Environmental Assessment - Biomedical Research Facility Ii, University Of Texas At Brownsville

Campus Master Plans (UTB/UTPA)

The Environmental Assessment (EA) was prepared in accordance with U.S. Department of Health and Human Services (HHS) guidelines, implementing the National Environmental Policy Act of 1969 (NEPA). The EA evaluates the environmental impacts of the proposed Biomedical Research Facility II (BRF II) laboratory expansion at the University of Texas at Brownsville/Texas Southmost College (UTB) campus. The NIH aims to partially fund this project, addressing the critical need for modern laboratory space to support transdisciplinary biomedical research. The No Action Alternative was also considered, but it would not meet the project’s purpose and need. Despite minor design changes, potential environmental impacts …

Molecular Systematics Of The Middle American Genus Hypopachus (Anura: Microhylidae), Eli Greenbaum, Eric N. Smith, Rafael O. De Sá

Biology Faculty Publications

We present the first phylogenetic study on the widespread Middle American microhylid frog genus Hypopachus. Partial sequences of mitochondrial (12S and 16S ribosomal RNA) and nuclear (rhodopsin) genes (1275 bp total) were analyzed from 43 samples of Hypopachus, three currently recognized species of Gastrophryne, and seven arthroleptid, brevicipitid and microhylid outgroup taxa. Maximum parsimony (PAUP), maximum likelihood (RAxML) and Bayesian inference (MrBayes) optimality criteria were used for phylogenetic analyses, and BEAST was used to estimate divergence dates of major clades. Population-level analyses were conducted with the programs NETWORK and Arlequin. Results confirm the placement of Hypopachus …

Full Issue

International Journal of Interpreter Education

No abstract provided.

Heart And Ventilatory Measures In Crayfish During Copulation, Richard M. Cooper, Heidi Schapker Finucane, Megan Adami, Robin L. Cooper

Biology Faculty Publications

Monitoring heart rate (HR) and ventilatory rate (VR) during defined sensory stimuli and during aggressive and submissive social interactions has provided additional information of a crayfish's physiological state which is not achieved by behavioral observations. In this study, the HR and VR of crayfish were monitored before, during and after the act of copulation in both heterosexual partners. The female crayfish abruptly reduces HR and VR during copulation but the male maintains HR and VR. After separation from copulation the female HR and VR are elevated, potentially paying back the O₂ debt. The tight relationship with HR and VR …

The Raf/Mek/Extracellular Signal-Regulated Kinase 1/2 Pathway Can Mediate Growth Inhibitory And Differentiation Signaling Via Androgen Receptor Downregulation In Prostate Cancer Cells., Seung-Keun Hong, Jin-Hwan Kim, Ming-Fong Lin, Jong-In Park

Journal Articles: Biochemistry & Molecular Biology

Upregulated ERK1/2 activity is correlated with androgen receptor (AR) downregulation in certain prostate cancer (PCa) that exhibits androgen deprivation-induced neuroendocrine differentiation, but its functional relevance requires elucidation. We found that sustained ERK1/2 activation using active Raf or MEK1/2 mutants is sufficient to induce AR downregulation at mRNA and protein levels in LNCaP. Downregulation of AR protein, but not mRNA, was blocked by proteasome inhibitors, MG132 and bortezomib, indicating that the pathway regulation is mediated at multiple points. Ectopic expression of a constitutively active AR inhibited Raf/MEK/ERK-mediated regulation of the differentiation markers, neuron-specific enolase and neutral endopeptidase, and the cyclin-dependent kinase …

Poem No. 106, November 2011, Huntsville Literary Association (Huntsvile, Ala.)

POEM

The Huntsville Literary Association was founded in 1967 to provide a community for writers, readers, and artists through workshops, readings, and educational programs. One of their key contributions is the literary magazine titled POEM which showcases original writings from both established and emerging writers. This issue features poems from various writers.

Elimination Of The Nlrp3-Asc Inflammasome Protects Against Chronic Obesity-Induced Pancreatic Damage, Yun Hee Youm, Ayinuer Adijiang, Bolormaa Vandanmagsar, David Burk, Anthony Ravussin, Vishwa Deep Dixit

Faculty Publications

Clinical evidence that the blockade of IL-1β in type-2 diabetic patients improves glycemia is indicative of an autoinflammatory mechanism that may trigger adiposity-driven pancreatic damage. IL-1β is a key contributor to the obesity-induced inflammation and subsequent insulin resistance, pancreatic β-cell dysfunction, and the onset of type 2 diabetes. Our previous studies demonstrated that the ceramides activate the Nod-like receptor family, pyrin domain containing 3 (Nlrp3) inflammasome to cause the generation of mature IL-1β and ablation of the Nlrp3 inflammasome in diet-induced obesity improves insulin signaling. However, it remains unclear whether the post-translational processing of active IL-1β in pancreas is regulated …

Genome-Wide Association Study Identifies Loci Influencing Concentrations Of Liver Enzymes In Plasma, J. C. Chambers, W. Zhang, J. Sehmi, X. Li, M. N. Wass, P. Van Der Harst, H. Holm, S. Sanna, M. Kavousi, S. E. Baumeister, L. J. Coin, G. Deng, C. Gieger, N. L. Heard-Costa, J. J. Hottenga, B. Kühnel, V. Kumar, V. Lagou, L. Liang, J. Luan, P. M. Vidal, I. Mateo Leach, P. F. O'Reilly, J. F. Peden, N. Rahmioglu, P. Soininen, E. K. Speliotes, X. Yuan, G. Thorleifsson, B. Z. Alizadeh, L. D. Atwood, I. B. Borecki, M. J. Brown

Faculty Publications

Concentrations of liver enzymes in plasma are widely used as indicators of liver disease. We carried out a genome-wide association study in 61,089 individuals, identifying 42 loci associated with concentrations of liver enzymes in plasma, of which 32 are new associations (P = 10-8 to P = 10-190). We used functional genomic approaches including metabonomic profiling and gene expression analyses to identify probable candidate genes at these regions. We identified 69 candidate genes, including genes involved in biliary transport (ATP8B1 and ABCB11), glucose, carbohydrate and lipid metabolism (FADS1, FADS2, GCKR, JMJD1C, HNF1A, MLXIPL, PNPLA3, PPP1R3B, SLC2A2 and TRIB1), glycoprotein biosynthesis …

The Efficacy Of Detecting Variants With Small Effects On The Affymetrix 6.0 Platform Using Pooled Dna, Charleston W.K. Chiang, Zofia K.Z. Gajdos, Joshua M. Korn, Johannah L. Butler, Rachel Hackett, Candace Guiducci, Thutrang T. Nguyen, Rainford Wilks, Terrence Forrester, Katherine D. Henderson, Loic Le Marchand, Brian E. Henderson, Christopher A. Haiman, Richard S. Cooper, Helen N. Lyon, Xiaofeng Zhu, Colin A. Mckenzie, Mark R. Palmert, Joel N. Hirschhorn

Faculty Publications

Genome-wide genotyping of a cohort using pools rather than individual samples has long been proposed as a cost-saving alternative for performing genome-wide association (GWA) studies. However, successful disease gene mapping using pooled genotyping has thus far been limited to detecting common variants with large effect sizes, which tend not to exist for many complex common diseases or traits. Therefore, for DNA pooling to be a viable strategy for conducting GWA studies, it is important to determine whether commonly used genome-wide SNP array platforms such as the Affymetrix 6.0 array can reliably detect common variants of small effect sizes using pooled …

The Remarkable Evolutionary History Of Endornaviruses, Marilyn J. Roossinck, Sead Sabanadzovic, Ryo Okada, Rodrigo A. Valverde

Faculty Publications

The family Endornaviridae contains several members from diverse hosts, including plants, fungi and oomycetes. They are found as large dsRNA elements with a nick in the coding strand. All members encode a conserved RNA-dependent RNA polymerase, but no other domain that is conserved among all members. Based on the conserved domain database comparison the various domains have different origins, indicating a highly modular evolutionary history. In some cases, domains with similar putative functions are found that are derived from different protein families, indicating convergent evolution for a required function. © 2011 SGM.

Trace Metals In Porewater Of Surface Sediments And Their Bioavailability In Jiaozhou Bay, Qingdao, China, Siyuan Ye, Edward A. Laws, Xigui Ding, Hongming Yuan, Guangming Zhao, Jin Wang

Faculty Publications

Samples of porewater and the edible tissue of mottled clams, Ruditapes variegatus, were collected simultaneously at each of 12 stations from Jiaozhou Bay near Qingdao, China, in June of 2003. Chemical analysis focused on trace metals and major elements. Porewater concentrations of Cd, Cr, Pb, and Zn were distributed in a bimodal fashion among the 12 stations, with mean concentrations at six of the stations being 8-32 times mean values at the other six stations. The concentrations of the same metals in clams were remarkably similar among stations, the coefficients of variation being only 12-37%. Calculations performed with the computer …

Open Access Advocacy: Beginning Steps In Colorado Academic Libraries, Wendy Highby

University Libraries Publications

This poster, presented by Wendy Highby at the Berlin 9 Open Access Conference in 2011, outlines the early efforts of Colorado academic libraries to advocate for open access. It highlights foundational initiatives including institutional repository development, policy adoption, faculty outreach, and collaborative planning. The poster emphasizes that effective open access advocacy requires aligning publication infrastructure, policy, promotion, and participatory planning, with examples from institutions such as Colorado State University, University of Northern Colorado, and University of Colorado Boulder.

Improving Commercial Fishing Access Rights In Western Australia, Department Of Fisheries

Fisheries Occasional Publications

Access Rights Working Group Report to the Hon Norman Moore, MLC Minister for Fisheries

Former Andrews Professor Ministers To Eunuchs Of India, Ashleigh Jardine

Lake Union Herald

No abstract provided.