Digital Commons Network^™

Induction Of B Cell Responses Upon Experimental Infection Of Neonatal Calves With Mycobacterium Avium Subsp. Paratuberculosis, Judith R. Stabel, John Bannantine, Shigetoshi Eda, Suelee Robbe-Austerman

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The objective of this study was to determine if experimental infection of neonatal calves with Mycobacterium avium subsp. paratuberculosis would invoke changes in the percentages of total B cells in the peripheral blood mononuclear cell population and of subpopulations of B cells as determined by CD5, CD25, and CD45RO markers during a 12-month period. Experimental infection groups included control (noninfected), oral (infected with M. avium subsp. paratuberculosis strain K-10), oral/DXM (pretreatment with dexamethasone before oral inoculation), i.p. (intraperitoneal inoculation), and oral/M (oral inoculation with mucosal scrapings from a cow with clinical disease) groups. Over the course of the study, the …

Past And Projected Future Changes In Snowpack And Soil Frost At The Hubbard Brook Experimental Forest, New Hampshire, Usa, John L. Campbell, Scott V. Ollinger, Gerald N. Flerchinger, Haley Wicklein, Katharine Hayhoe, Amey S. Bailey

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Long-term data from the Hubbard Brook Experimental Forest in New Hampshire show that air temperature has increased by about 1°C over the last half century. The warmer climate has caused significant declines in snow depth, snow water equivalent and snow cover duration. Paradoxically, it has been suggested that warmer air temperatures may result in colder soils and more soil frost, as warming leads to a reduction in snow cover insulating soils during winter. Hubbard Brook has one of the longest records of direct field measurements of soil frost in the United States. Historical records show no long-term trends in maximum …

Regulatory Control Of High Levels Of Carotenoid Accumulation In Potato Tubers, Xiangjun Zhou, Ryan Mcquinn, Zhangjun Fei, Anne-Marie A. Wolters, Joyce Van Eck, Charles Brown, James J. Giovannoni, Li Li

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Potato (Solanum tuberosum L.) tubers contain a wide range of carotenoid contents. To decipher the key factors controlling carotenoid levels in tubers, four potato lines (Atlantic, Désirée, 91E22 and POR03) were examined by a combination of biochemical, molecular and genomics approaches. These lines contained incremental levels of carotenoids, which were found to be associated with enhanced capacity of carotenoid biosynthesis as evident from norflurazon treatment. Microarray analysis of high and low carotenoid lines (POR03 versus Atlantic) revealed 381 genes that showed significantly differential expression. The carotenoid metabolic pathway genes b-carotene hydroxylase 2 (BCH2) and b-carotene hydroxylase 1 (BCH1), along …

Invited Commentary: Dietary Pattern Analysis, Fumiaki Imamura, Paul F. Jaques

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The analytic approaches used in nutritional epidemiology for dietary pattern analyses share common characteristics with those of genetic epidemiology. In this issue of the Journal, Gorst-Rasmussen et al. (Am J Epidemiol. 2011;173(10):1097–1104) discuss one such approach. Application of methods used in genetic pattern analyses to nutritional epidemiology could prove valuable but raises important issues that need to be considered because dietary and genetic studies often address different types of questions in analyzing interrelated variables. These different aims require statistical methods that assume different characteristics of the underlying patterns. The authors briefly describe such differences to facilitate interpretation and …

Physiology And Endocrinology Symposium: Role Of The Oviduct In Maintaining Sustained Fertility In Hens, M. R. Bakst

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In poultry, sperm transferred by natural mating or AI into the distal end of the vagina immediately begin their ascent to the uterovaginal junction (UVJ) at the anterior end of the vagina. However, due to an intense selection process in the vagina, less than 1% of the sperm transferred actually reach the UVJ. Those sperm that do reach the UVJ enter numerous tubular invaginations of the surface epithelium of the vagina located in the UVJ mucosa, collectively referred to as the sperm-storage tubules (SST). Sperm residing in the SST lumen are capable of surviving up to several weeks while retaining …

26th Hohenheim Consensus Conference, September 11, 2010 Scientific Substantiation Of Health Claims: Evidence-Based Nutrition, Hans Konrad Biesalski, Peter J. Aggett, Robert Anton, Paul S. Bernstein, Jeffrey Blumberg, Robert P. Heaney, Jeya Henry, John M. Nolan, David P. Richardson, Ben Van Ommen, Renger F. Witkamp, Ger T. Rijkers, Iris Zöllner

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Objective: The objective was to define the term evidence based nutrition on the basis of expert discussions and scientific evidence.

Methods and procedures: The method used is the established Hohenheim Consensus Conference. The term “Hohenheim Consensus Conference” defines conferences dealing with nutrition-related topics. The major aim of the conference is to review the state of the art of a given topic with experts from different areas (basic science, clinicians, epidemiologists, etc.). Based on eight to 12 questions, the experts discuss short answers and try to come to a consensus. A scientifically based text is formulated that justifies the …

Response Of Tribolium Castaneum And Tribolium Confusum Adults To Vertical Black Shapes And Its Potential To Improve Trap Capture, Altair A. Semeao, James F. Campbell, R. Jeff Whitworth, Phillip E. Sloderbeck

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Tribolium castaneum and Tribolium confusum can be monitored in food processing facilities using traps baited with pheromones and kairomones, but beetle response to traps might be enhanced by adding visual cues. Against a white background, T. castaneum adults were more likely to visit black pillars than white pillars when presented with a choice (e.g., 73% of beetles visited black and 17% visiting white pillar), and visits to black pillars increased with pillar height. When tested against a black background, beetles did not show a significant preference for either color pillar regardless of height. When comparing beetle’s captures in pheromone/kairomone baited …

Effect Of Maternal Restraint Stress During Gestation On Temporal Lipopolysaccharide-Induced Neuroendocrine And Immune Responses Of Progeny, C.T. Collier, P.N. Williams, J.A. Carroll, T.H. Welsh Jr, J.C. Laurenz

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The impact of gestational dam restraint stress on progeny immune and neuroendocrine temporal hormone responses to lipopolysaccharide (LPS) challenge was assessed. Maternal stress (5-min snout snare restraint stress during days 84 to 112 of gestation) increased (P < 0.05) the magnitude of tumor necrosis factor (TNF)-α, interleukin-6, epinephrine (E), norepinephrine, and serum amyloid A (SAA) production following LPS infusion in the offspring. Moreover, these effects appear to be dependent on gender for TNF-α, E, and cortisol production. However, maternal stress did not affect (P < 0.05) the normalization of proinflammatory cytokines or neuroendocrine hormones produced following LPS. Collectively, these results indicate that maternal stress impacts aspects of the proinflammatory cytokine and stress hormone response in their progeny following LPS dosing of the offspring. This response is potentially responsible in part for the resultant changes to SAA production. Because several of the changes observed here are dependent on pig gender, these results are also the first evidence that inherent epigenetic factors coupled with maternal stress impact the cumulative response to stress and LPS in young pigs.

Influence Of Ensiling Time And Inoculation On Alteration Of The Starch-Protein Matrix In High-Moisture Corn, P. C. Hoffman, N. M. Esser, R. D. Shaver, W. K. Coblentz, M. P. Scott, A. L. Bodnar, R. J. Schmidt, R. C. Charley

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The fates of hydrophobic zein proteins, which encapsulate corn starch to create vitreous endosperm, have not been investigated in high-moisture corn (HMC). To assess influences of ensiling time and inoculation on zein proteins in HMC, quadruplicate samples of 2 random corn hybrids (A and B), containing 25.7 and 29.3% moisture, were ground, inoculated with (I) or without 600,000 cfu/g of Lactobacillus buchneri 40788 (Lallemand Animal Nutrition, Milwaukee, WI), and ensiled for 0, 15, 30, 60, 120, and 240 d. Nutrient composition [crude protein (CP), starch, acid detergent fiber, and neutral detergent fiber], fermentation (pH, lactate, and acetate), and protein degradation …

Evaluation Of Two Mutants Of Mycobacterium Avium Subsp. Paratuberculosis As Candidates For A Live Attenuated Vaccine For Johne’S Disease, Kun Taek Park, Andrew J. Allen, John P. Bannantine, Keun Seok Seo, Mary J. Hamilton, Gaber S. Abdellrazeq, Heba M. Rihan, Amanda Grimm, William C. Davis

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Control of Johne’s disease, caused by Mycobacterium avium subsp. paratuberculosis, has been difficult because of a lack of an effective vaccine. To address this problem we used targeted gene disruption to develop candidate mutants with impaired capacity to survive ex vivo and in vivo to test as a vaccine. We selected relA and pknG, genes known to be important virulence factors in Mycobacterium tuberculosis and Mycobacterium bovis, for initial studies. Deletion mutants were made in a wild type Map (K10) and its recombinant strain expressing the green fluorescent protein (K10-GFP). Comparison of survival in an ex vivo …

A Comparison Of Internal And External Lipids Of Nondiapausing And Diapause Initiation Phase Adult Colorado Potato Beetles, Leptinotarsa Decemlineata, George D. Yocum, James S. Buckner, Charlotte L. Fatland

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The Colorado potato beetle, Leptinotarsa decemlineata, reared under diapause-inducing conditions will emerge fromthe soil as an adult and enter the diapause initiation phase, a periodwheremetabolic reserves are stockpiled before the beetles enter the nonfeeding diapause maintenance phase. Internal and external lipids were characterized during the diapause initiation phase (IP) and compared to the lipid profiles of nondiapausing adults. The primary internal lipids of both diapause IP and nondiapausing adults are triacylglycerols. Only trace amounts of internal lipids were detected in day 1 diapause IP adults. A dramatic increase in internal lipids was observed between day 7 and day 15 …

Quality And Agronomic Effects Of Three High-Molecular-Weight Glutenin Subunit Transgenic Events In Winter Wheat, Robert A. Graybosch, Bradford Seabourn, Yuanhong R. Chen, Ann E. Blechl

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Quality and agronomic effects of three transgenic high molecular weight glutenin subunit (HMW-GS) events were characterized in advanced generation breeding lines of hard winter wheat (Triticum aestivum L.) in three Nebraska crop years. Two or the transgenic events studied, Dy10-E and B52a-6, ovenexpress HMW-GS IDY10, while the third event, Dx5 +Dy10-H, overexpresses HMW-GS IDx5 and to a much lesser extent. IDY10. In addition, novel proteins possessing solubility characteristics defined as HMW-GS were present in Dx5+Dy10-H and B52a-6. Average grain yield of lines derived from the three transgenic events was statistically lower than that of a group of control cultivars …

Evaluation Of Propane Combustion Traps For The Collection Of Phlebotomus Papatasi (Scopoli) In Southern Israel, Daniel Kline, Gunter Miller, Jerome Hogsette

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In this study, we evaluated the efficacy of eleven commercial models of propane combustion traps for catching male and female Phlebotomus papatasi. The traps differed in physical appearance, amount of carbon dioxide produced and released, type and location of capturing device, and the method by which the trap suction fans were powered. The traps tested were the Mosquito Magnet^™(MM)-Pro, MM-Liberty, MM-Liberty Plus, MM-Defender, SkeeterVac^®(SV)-35, SV-27, Mosquito Deleto™(MD)-2200, MD-2500, MT150-Power Trap, and two models of The Guardian Mosquito Traps (MK-01 and MK-12). All trap models except the SV-35, the SV-27, the MD-2500, and the MK-12 attracted …

Improving Animal Welfare And Reducing Animal Use For Veterinary Vaccine Potency Testing: State Of The Science And Future Directions, William S. Stokes, Karen Brown, Jodie Kulpa-Eddy, Geetha Srinivas, Marlies Halder, Hans Draayer, Jeffrey Galvin, Ivo Claassen, Glen Gifford, Ralph Woodland, Vivian Doelling, Brett Jones

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Veterinary vaccines contribute to improved human and animal health and welfare by preventing diseases and deaths caused by a wide range of infectious agents. However, testing necessary to ensure vaccine effectiveness and safety can involve large numbers of animals and significant pain and distress. NICEATM and ICCVAM convened an international workshop to review the state of the science of human and veterinary vaccine potency and safety testing methods and to identify opportunities to advance new and improved methods that can further reduce, refine, and replace animal use. This workshop report is the fourth in a series of six, and addresses …

Alpine Russet: A Potato Cultivar Having Long Tuber Dormancy Making It Suitable For Processing From Long-Term Storage, Jonathan L. Whitworth, Richard G. Novy, Jeffrey C. Stark, Joseph J. Pavek, Dennis L. Corsini, Steven L. Love, Nora Olsen, Sanjay K. Gupta, Tina Brandt, M. Isabel Vales, Alvin R. Mosley, Solomon Yilma, Steve R. James, Dan C. Hane, Brian A. Charlton, Clinton C. Shock, N. Richard Knowles, Mark J. Pavek, Jeffrey S. Miller, Charles R. Brown

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Abstract Alpine Russet is a later maturing, oblong-long, lightly russeted potato cultivar, notable for having tuber dormancy comparable to Russet Burbank. Processing quality of Alpine Russet from long-term storage is superior to Russet Burbank, with low percent reducing sugars and uniform fry color due to a low percentage difference of sugars between the bud and stem ends. Alpine Russet yields were comparable to Russet Burbank in early harvest trials and were comparable or significantly larger in late harvest trials depending on the location. At two late season locations, Alpine Russet had the largest total and percent No. 1 yields and …

A Spatially Explicit Degree-Day Model Of Rift Valley Fever Transmission Risk In The Continental United States, Sarah K. Konrad, Scott N. Miller, Will K. Reeves

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A spatially explicit degree-day model was used to evaluate the risk of Rift Valley fever virus (RVFV) transmission by mosquitoes to humans and livestock within five target states in the continental United States: California, Minnesota, Nebraska, New York, and Texas. A geographic information system was used to model potential virus transmission based on a 12-day moving window assessment of the extrinsic incubation period theorized for RVFV in the United States. Risk of potential virus transmission in each state was spatially evaluated on a 10-km grid using average historical daily temperature data from 1994 to 2003. The highest levels of transmission …

A Threat Taxonomy For Mhealth Privacy, David Kotz

Dartmouth Scholarship

Networked mobile devices have great potential to enable individuals (and their physicians) to better monitor their health and to manage medical conditions. In this paper, we examine the privacy-related threats to these so-called \emphmHealth\/ technologies. We develop a taxonomy of the privacy-related threats, and discuss some of the technologies that could support privacy-sensitive mHealth systems. We conclude with a brief summary of research challenges.

The Seaside Resort Towns Of Cape May And Atlantic City, New Jersey Development, Class Consciousness, And The Culture Of Leisure In The Mid To Late Victorian Era, Tatyana Ressetar

Electronic Theses and Dissertations

"Victorianism" is a highly controversial, sometimes ironic, term penned by historians throughout various works that has come to hold dramatic weight in both its meaning and its influence. Though the term is usually most closely associated with nineteenth century England, Victorianism was a highly influential movement in American culture simultaneously as well, specifically in the spheres of home, work, and play. Of those, "play," or leisure, is undoubtedly the least explored, especially before the latter decades of the twentieth century. Prior to this period, most literature about the Victorians, with the exception of a few works, only dealt with masculinity, …

A Laudable Ambition Fired Her Soul Conduct Fiction Helps Define Republican Womanhood, Female Communities, And Women's Education In The Works Of Judith Sargent Murray, Hannah Webster Foster, And Susanna Haswell Rowson, Jessica Crystal Workman

Electronic Theses and Dissertations

This study examines the major works of Judith Sargent Murray, Hannah Webster Foster, and Susanna Haswell Rowson, three major writers of the 1790s whose writing responds to the ideologies of the early American Republic. I suggest that Murray, Foster, and Rowson write conduct fiction which responds to the changing attitudes toward women and education after the American Revolution. Using fiction, these authors comment on the republican woman, the need for women’s education, and the necessity for women to gather in communities for support. Despite the prevailing notion that reading too many novels would corrupt young women, Judith Sargent Murray’s novella, …

Stable Expression Of Tuberculosis Vaccine Antigen In Lettuce Chloroplasts, Priya Saikumar Lakshmi

Electronic Theses and Dissertations

Tuberculosis (TB) is caused by Mycobacterium tuberculosis and is one of the leading reasons of death by an infectious bacterial pathogen. The development of TB vaccines has been recognized as a major public health priority by the World Health Organization. In this study, a potential candidate antigen, ESAT-6 (6 kDa early secretory antigenic target) was fused with cholera toxin B subunit (CTB). Transplastomic lettuce plants were generated expressing these fusion proteins. Site-specific transgene integration into the chloroplast genome was confirmed by polymerase chain reaction and Southern blot analysis. In transplastomic leaves, expression levels of fusion protein (CTB-ESAT6) varied depending upon …

Evolutionary Relationships Among Staphylococci And The Prevention Of Staphylococcus Aureus Nasal Colonization, Ryan Paul Lamers

Electronic Theses and Dissertations

Staphylococcus is a significant cause of human infection and mortality, worldwide. Currently, there are greater than 60 taxa within Staphylococcus, and nearly all are pathogenic. The collective potential for virulence among species of Staphylococcus heightens the overall clinical significance of this genus and argues for a thorough understanding of the evolutionary relationships among species. Within Staphylococcus, aureus is the most common cause of human infection, where nasal carriage of this bacterium is a known risk factor for autoinfection. The predisposition to infection by nasal carriers of S. aureus, and the ease with which strains are transferred between individuals, suggests that …

Prediction Of Survival Of Early Stages Lung Cancer Patients Based On Er Beta Cellular Expressions And Epidemiological Data, Evgeny Martinenko

Electronic Theses and Dissertations

We attempted a mathematical model for expected prognosis of lung cancer patients based on a multivariate analysis of the values of ER-interacting proteins (ERbeta) and a membrane bound, glycosylated phosphoprotein MUC1), and patients clinical data recorded at the time of initial surgery. We demonstrate that, even with the limited sample size available to use, combination of clinical and biochemical data (in particular, associated with ERbeta and MUC1) allows to predict survival of lung cancer patients with about 80% accuracy while prediction on the basis of clinical data only gives about 70% accuracy. The present work can be viewed as a …

Indirect Estimates Of Gene Flow And Its Conservation Implications In The Striped Newt (Notophthalmus Perstriatus), Sarah Elizabeth May

Electronic Theses and Dissertations

This study used indirect methods to estimate patterns of gene flow in a rare salamander species, the striped newt (Notophthalmus perstriatus). First, we used combined genetic and ecological methods to determine whether populations that appear to exist in two regions separated by 125 km, exhibited genetic and ecological distinctness such that the regions demarcate separate conservation units. Using mtDNA (cyt-b), we found that haplotypes were shared between localities within each region but none were shared between regions. Niche-based distribution modeling revealed significant differences in the ecological setting between the two regions. In combination, the absence of evidence for recent genetic …

The Effects Of Urbanization On Cypress (Taxodium Distichum) In Central Florida, Lisa A. Mccauley

Electronic Theses and Dissertations

Urbanization is accelerating in the United States and is contributing to fragmentation of natural habitats, causing changes in species composition and declines in native species. Human population growth in Orlando is typical of growth in the southeastern United States and throughout the range of cypress (Taxodium distichum). Orlando has numerous isolated cypress wetlands, called cypress domes, and many remain among the current urbanized area. This makes Orlando ideal to study the effects of urbanization on cypress domes. Specifically, I tested how urbanization and its effects on fragmentation, hydrology, and fire regime) affected (a) the numbers and spatial pattern of cypress …

Synthesis, Characterization And Antibacterial Activity Of Silver Embedded Silica Nanoparticle/Nanogel Formulation, Roseline Menezes

Electronic Theses and Dissertations

The antibacterial property of silver (Ag) has been known since ancient time. It is reported in the literature that silver nanoparticles (AgNPs) exhibit improved antibacterial properties in comparison to silver ions of equivalent metallic Ag concentration. Such improvement in antibacterial activities is due to the high surface area to volume ratio of AgNPs (which facilitates interaction with the bacterial cells), increased release of silver ions and direct intra-cellular uptake of AgNPs leading to localized release of Ag ions. To date, over 300 consumer products containing AgNPs are available in the market and the inventory is rapidly expanding. The antibacterial efficacy …

Expression And Functional Evaluation Of Exendin 4 Fused To Cholera Toxin B Subunit In Tobacco Chloroplast To Treat Type 2 Diabetes, Ramya Nityanandam

Electronic Theses and Dissertations

The prevalence of type 2 diabetes has been steadily increasing around the globe. Glucagon like peptide (GLP-1), a powerful incretin increases insulin secretion in a glucose dependent manner. But GLP-1 is subjected to rapid enzymatic degradation (half-life: 2 min in circulation). The commercially available GLP-1 analog, exenatide has a longer half life with potent insulinotropic effects (about 2.4 hr) which requires cold storage and daily subcutaneous injections. In this study, exendin 4 (EX4), lizard derived GLP-1R agonist, was expressed as cholera toxin B subunit (CTB)-fusion protein in chloroplasts of tobacco to facilitate transmucosal delivery in the gut by utilizing the …

Direct, Hands-On Or Inquiry Instruction A Study Of Instructional Sequencing And Motivation In The Science Classroom, Jamie Vander Wiede

Electronic Theses and Dissertations

Currently, a debate exists between the strengths and weaknesses of direct and inquiry instruction. Inquiry instruction is related to positive effect on learner motivation whereas supporters of direct instruction point to its ability to adequately support learners’ working memories (Hmelo-Silver, Duncan, & Chinn, 2007; Kirschner, Sweller, & Clark, 2006; Kuhn, 2007; Sweller, 1988). This study examined the possibility of combining the best features of both inquiry and direct instruction by sequencing them together. A two-part lesson on electrical circuits was presented in three separate sequences of instruction to middle school students to determine if differences in student motivation and academic …

Media Effects On Body Image In The Context Of Environmental And Internal Influences What Matters Most?, Kristen E. Vanvonderen

Electronic Theses and Dissertations

Media effects on body dissatisfaction is a long-studied issue; however, aspects of the research – such as those regarding cultivation theory and its effects on body image – are unclear or incomplete. This study attempts to clarify the relationship between cultivation and body dissatisfaction. Besides cultivation, social comparison theory is also examined because upward comparisons with media images and peers can shape and reinforce body image attitudes as well. Additionally, the study examines the connection between media and body dissatisfaction by looking at a broader social context – one that includes other social/environmental influences, such as peer and parental attitudes, …

Expression Of Trichoderma Reesei Beta]-Mannanase In Tobacco Chloroplasts And Its Utilization In Lignocellulosic Woody Biomass Hydrolysis, Pankaj M. Agrawal

Electronic Theses and Dissertations

Lignocellulosic ethanol offers a promising alternative to conventional fossil fuels. One among the major limitations in the lignocellulosic biomass hydrolysis is unavailability of efficient and environmentally biomass degrading technologies. Plantbased production of these enzymes on large scale offers a cost effective solution. Cellulases, hemicellulases including mannanases and other accessory enzymes are required for conversion of lignocellulosic biomass into fermentable sugars. β- mannanase catalyzes endo-hydrolysis of the mannan backbone, a major constituent of woody biomass. In this study, man1 gene encoding β-mannanase was isolated from Trichoderma reesei and expressed via the chloroplast genome. PCR and Southern hybridization analysis confirmed the site-specific …

The Effects Of Presentation Mode And Pace On Learning Immunology With Computer Simulation A Cognitive Evaluation Of A Multimedia Learning Resource, Radakovich Kristy Bradley

Electronic Theses and Dissertations

Multimedia learning tools have the potential to benefit instructors and learners as supplemental learning materials. However, when such tools are designed inappropriately, this can increase cognitive taxation and impede learning, rendering the tools ineffective. Guided by the theoretical underpinnings provided by cognitive load theory and the cognitive theory of multimedia learning, this study sought to empirically evaluate the effectiveness of a multimedia simulation tool aimed at teaching immunology to novices in an instructional setting. The instructional mode and pace of the tool were manipulated, the three levels of each variable yielding nine experimental groups. The effects of mode and pace …