Digital Commons Network^™

Dur3 Is The Major Urea Transporter In Candida Albicans And Is Co-Regulated With The Urea Amidolyase Dur1,2, Dhammika H. M. L. P Navarathna, Aditi Das, Joachim Morschhauser, Kenneth W. Nickerson, David D. Roberts

Kenneth Nickerson Papers

Hemiascomycetes, including the pathogen Candida albicans, acquire nitrogen from urea using the urea amidolyase Dur1,2, whereas all other higher fungi use primarily the nickel-containing urease. Urea metabolism via Dur1,2 is important for resistance to innate host immunity in C. albicans infections. To further characterize urea metabolism in C. albicans we examined the function of seven putative urea transporters. Gene disruption established that Dur3, encoded by orf 19.781, is the predominant transporter. [¹⁴C]Urea uptake was energy-dependent and decreased approximately sevenfold in a dur3D mutant. DUR1,2 and DUR3 expression was strongly induced by urea, whereas the other putative transporter genes …

Arabidopsis G-Protein Interactome Reveals Connections To Cell Wall Carbohydrates And Morphogenesis, Karsten Klopffleisch, Nguyen Phan, Kelsey Augustin, Robert S. Bayne, Katherine S. Booker, Jose R. Botella, Nicholas C. Carpita, Tyrell Carr, Jin-Gui Chen, Thomas Ryan Cooke, Arwen Frick-Cheng, Erin J. Friedman, Brandon Fulk, Michael G. Hahn, Kun Jiang, Lucia Jorda, Lydia Kruppe, Chenggang Liu, Justine Lorek, Maureen C. Mccann, Antonio Molina, Etsuko N. Moriyama, M. Shahid Mukhtar, Yashwanti Mudgil, Sivakumar Pattathil, John Schwarz, Steven Seta, Matthew Tan, Ulrike Temp, Yuri Trusov, Daisuke Urano, Bastian Welter, Jing Yang, Ralph Panstruga, Joachim F. Uhrig, Alan M. Jones

School of Biological Sciences: Faculty Publications

The heterotrimeric G-protein complex is minimally composed of Ga, Gb, and Gc subunits. In the classic scenario, the G-protein complex is the nexus in signaling from the plasma membrane, where the heterotrimeric G-protein associates with heptahelical G-protein-coupled receptors (GPCRs), to cytoplasmic target proteins called effectors. Although a number of effectors are known in metazoans and fungi, none of these are predicted to exist in their canonical forms in plants. To identify ab initio plant G-protein effectors and scaffold proteins, we screened a set of proteins from the G-protein complex using two-hybrid complementation in yeast. After deep and exhaustive interrogation, we …

Nmr Analysis Of A Stress Response Metabolic Signaling Network, Bo Zhang, Steven M. Halouska, Charles E. Schiaffo, Marat R. Sadykov, Greg A. Somerville, Robert Powers

Robert Powers Publications

We previously hypothesized that Staphylococcus epidermidis senses a diverse set of environmental and nutritional factors associated with biofilm formation through a modulation in the activity of the tricarboxylic acid (TCA) cycle. Herein, we report our further investigation of the impact of additional environmental stress factors on TCA cycle activity and provide a detailed description of our NMR methodology. S. epidermidis wild-type strain 1457 was treated with stressors that are associated with biofilm formation, a sub-lethal dose of tetracycline, 5% NaCl, 2% glucose and autoinducer-2 (AI-2). As controls and to integrate our current data with our previous study, 4% ethanol stress …

Postmodernidad Y Metaficción En Hipótesis (1975), Iker González-Allende

Spanish Language and Literature Papers

En su tercera novela, Guerra Garrido explora las posibilidades de que una máquina produzca una narrativa de ficción. El título implica precisamente la duda de si este tipo de escritura es factible y apunta el carácter experimental y lúdico que encierra la novela. Sin embargo, Hipótesis va más allá de las vicisitudes de un personaje escritor en busca de los últimos avances tecnológicos para la consecución de una obra enteramente nueva y original. Los otros cuatro personajes que aparecen en sus páginas adquieren tanta relevancia argumental como el escritor, a pesar de que se convierten en los protagonistas de la …

Speech Corpus Generation From Dvds Of Movies And Tv Series, Veton Z. Kepuska, Pattarapong Rojanasthien

Journal of International Technology and Information Management

Speech corpus is a database of audio files containing spoken words/sentences and text transcriptions. In this work we present a data collection system for creating speech corpora from movies and TV series DVDs. Corpus generation from these DVDs is significantly lower- cost solution comparing to conventional way of obtaining a speech corpus. In addition, it also takes a shorter amount of time to collect the data and processes it into a corpus. In order to be able to perform this operation the Data Collection Toolkit is introduced. This toolkit is an application developed using C# .Net Framework 3.5 in Visual …

Characterizing Rna Dynamics At Atomic Resolution Using Solution-State Nmr Spectroscopy, Jameson R. Bothe, Evgenia N. Nikolova, Catherine D. Eichhorn, Jeetender Chugh, Alexandar L. Hansen, Hashim M. Al-Hashimi

Department of Chemistry: Faculty Publications

Many recently discovered non-coding RNAs do not fold into a single native conformation, but rather, sample many different conformations along their free energy landscape to carry out their biological function. Unprecedented insights into the RNA dynamic structure landscape are provided by solution-state NMR techniques that measure the structural, kinetic, and thermodynamic characteristics of motions spanning picosecond to second timescales at atomic resolution. From these studies a basic description of the RNA dynamic structure landscape is emerging, bringing new insights into how RNA structures change to carry out their function as well as applications in RNA-targeted drug discovery and RNA bioengineering.

Comparative Manufacture And Cell-Based Delivery Of Antiretroviral Nanoformulations, Shantanu Balkundi, Ari S. Nowacek, Ram S. Veerubhotla, Han Chen, Andrea Martinez-Skinner, Upal Roy, R. Lee Mosley, Georgette Kanmogne, Xinming Liu, Alexander V. Kabanov, Tatiana Bronich, Joellyn Mcmillan, Howard E. Gendelman

Nebraska Center for Biotechnology: Faculty and Staff Publications

Nanoformulations of crystalline indinavir, ritonavir, atazanavir, and efavirenz were manufactured by wet milling, homogenization or sonication with a variety of excipients. The chemical, biological, immune, virological, and toxicological properties of these formulations were compared using an established monocyte-derived macrophage scoring indicator system. Measurements of drug uptake, retention, release, and antiretroviral activity demonstrated differences amongst preparation methods. Interestingly, for drug cell targeting and antiretroviral responses the most significant difference among the particles was the drug itself. We posit that the choice of drug and formulation composition may ultimately affect clinical utility.

A Perspective On Thirty Years Of The Webb, Pearman And Leuning Density Corrections, Xuhui Lee, William J. Massman

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

The density correction theory of Webb et al. (1980, Q J Roy Meteorol Soc 106: 85–100, hereafter WPL) is a principle underpinning the experimental investigation of surface fluxes of energy and masses in the atmospheric boundary layer. It has a long-lasting influence in boundary-layer meteorology and micrometeorology, and the year 2010 marks the 30th anniversary of the publication of the WPL theory. We provide here a critique of the theory and review the research it has spurred over the last 30 years. In the authors’ opinion, the assumption of zero air source at the surface is a fundamental novelty that …

A Qualitative And Quantitative Analysis Of Risk Perception And Treatment Options As Related To Wildfires In The Usda Fs Region 3 National Forests, Ingrid M. Martin, Wade E. Martin, Carol B. Raish

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

As the incidence of devastating fires rises, managing the risk posed by these fires has become critical. This report provides important information to examine the ways that different groups or disaster subcultures develop the mentalities or perceived realities that affect their views and responses concerning risk and disaster preparedness. Fire risk beliefs and attitudes of individuals and groups from four geographic areas in the Southwest (U.S. Department of Agriculture, Forest Service, Region 3, Arizona and New Mexico) surrounding the Kaibab, Tonto, Santa Fe, and Lincoln National Forests are presented. Using both quantitative and qualitative methods, we collected information from three …

School-Based Extracurricular Activity Involvement And Adolescent Self-Esteem: A Growth-Curve Analysis, Lisa A. Kort-Butler, Kellie J. Hagewen

Department of Sociology: Faculty Publications

Research on adolescent self-esteem indicates that adolescence is a time in which individuals experience important changes in their physical, cognitive, and social identities. Prior research suggests that there is a positive relationship between an adolescent’s participation in structured extracurricular activities and well-being in a variety of domains, and some research indicates that these relationships may be dependent on the type of activities in which adolescents participate. Building on previous research, a growth-curve analysis was utilized to examine self-esteem trajectories from adolescence (age 14) to young adulthood (age 26). Using 3 waves of data from National Longitudinal Study of Adolescent Health …

Alcohol Use Trajectories And Problem Drinking Over The Course Of Adolescence: A Study Of North American Indigenous Youth And Their Caretakers, Jacob E. Cheadle, Les B. Whitbeck

Department of Sociology: Faculty Publications

This study investigated the links between alcohol use trajectories and problem drinking (DSM-IV abuse/dependence) using five waves of data from 727 North American Indigenous adolescents between 10–17 years from eight reservations sharing a common language and culture. Growth mixture models linking fundamental causes, social stressors, support, and psychosocial pathways to problem drinking via alcohol use trajectories over the early life course were estimated. Results indicated that 20% of the adolescents began drinking at 11–12 years of age and that another 20% began drinking shortly thereafter. These early drinkers were at greatly elevated risk for problem drinking, as were those who …

Introduction: Charlotte Perkins Gilman On The Sociology Of Families, Marriages, And Children, Michael R. Hill

Department of Sociology: Faculty Publications

Charlotte Perkins Gilman died in 1935, but she remains today a provocative sociological writer; she makes us think, argue, and question our preconceptions, especially with regard to marriage and family. Several posthumous volumes of Gilman’s work have been produced and it has been my pleasure to help present three of Gilman’s (1997, 2002, 2004) major sociological writings to new generations of readers in English. As noted in the preface, it has been a special honor to acquaint an audience of Italian readers with a selection of her powerful writings on families, marriages, and children.1 The present volume joins a small …

A Reassessment Of The Role Of Propagule Pressure In Influencing Fates Of Passerine Introductions To New Zealand, Michael P. Moulton, Wendell P. Cropper Jr., Michael L. Avery

United States Department of Agriculture Wildlife Services: Staff Publications

Several studies have argued that principal factor in determining the fate of bird introductions is introduction effort. In large part, these studies have emerged from analyses of historical records from a single place—New Zealand. Here we raise two concerns about these conclusions. First, we argue that although many bird species were introduced repeatedly to New Zealand, in many cases the introductions apparently occurred only after the species were already successfully naturalized. The inclusion of such seemingly superfluous introductions may exaggerate the importance of propagule pressure. And second, we question the reliability of the records themselves. In many cases these records …

Surface Complexation Modeling For Predicting Solid Phase Arsenic Concentrations In The Sediments Of The Mississippi River Valley Alluvial Aquifer, Arkansas, Usa, Md. Salah U. Sharif, Ralph K. Davis, Kenneth F. Steele, Burmshik Kim, Phillip D. Hays, Tim M. Kresse, John A. Fazio

United States Geological Survey: Staff Publications

The potential health impact of As in drinking water supply systems in the Mississippi River Valley alluvial aquifer in the state of Arkansas, USA is significant. In this context it is important to understand the occurrence, distribution and mobilization of As in the Mississippi River Valley alluvial aquifer. Application of surface complexation models (SCMs) to predict the sorption behavior of As and hydrous Fe oxides (HFO) in the laboratory has increased in the last decade. However, the application of SCMs to predict the sorption of As in natural sediments has not often been reported, and such applications are greatly constrained …

Correlation Of Omega-3 Fatty Acids Intakes With Acculturation And Socioeconomic Status In Midwestern Latinas, Karina R. Lora, Nancy M. Lewis, Kent M. Eskridge, Kaye Stanek-Krogstrand, Daryl A. Travnicek

Department of Nutrition and Health Sciences: Faculty Publications

Background: Low socioeconomic status (SES) and acculturation of Latino immigrants in the U.S. are linked to a decrease in diet quality. Methods: Interviews were conducted with 162 first-generation Latinas to examine the association of SES and acculturation with intake of omega-3 (n − 3) fatty acids. Each participant provided dietary intake by use of a validated n − 3 food frequency questionnaire administered twice, 4 weeks apart, three 24-h recalls, sociodemographic information and completed the 5-item Short Acculturation Scale. Results: Mean intakes of Total n − 3, α-linolenic acid (ALA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) (g/d) were …

Ray-Traced Simulation Of Radiation Pressure For Optical Lift, Timothy Peterson

Theses

When light refracts at a surface, it changes the direction and intensity of the light rays. By Newton's 3rd law, this process imparts a small momentum to the object. This effect can be exploited to manipulate very small objects, by carefully selecting the shape and optical properties of the object. This thesis describes a computational model based on the laws of physics to determine the forces and torques resulting from light interacting with a hemicylindrical particle. This model has been successfully used to predict for the first time a phenomenon called optical lift.

Addressing The Gender Gap In Federal Law Enforcement Professions: The Motivational Attraction Of Women In Computing, Maegan Stanek

Theses

Nationwide, federal law enforcement agencies seek candidates with specialized skill sets and more advanced educational knowledge for employment. In view of this, it was proven, after much open source research into federal agencies' qualification requirements, that those who possess a background in select areas, specifically computer science, have a better opportunity for employment with federal law enforcement agencies, particularly in the computing sector; although, in many cases, work experience can be substituted for a college degree [7]. For this reason, many women are not involved in, nor apply for, computing professions within federal law enforcement agencies. A significant step in …

Sirt1 Modulates Aggregation And Toxicity Through Deacetylation Of The Androgen Receptor In Cell Models Of Sbma, Heather L. Montie, Richard G. Pestell, Diane E. Merry

PCOM Scholarly Works

Posttranslational protein modifications can play a major role in disease pathogenesis; phosphorylation, sumoylation, and acetylation modulate the toxicity of a variety of proteotoxic proteins. The androgen receptor (AR) is substantially modified, in response to hormone binding, by phosphorylation, sumoylation, and acetylation; these modifications might thus contribute to DHT-dependent polyglutamine (polyQ)-expanded AR proteotoxicity in spinal and bulbar muscular atrophy (SBMA). SIRT1, a nuclear protein and deacetylase of the AR, is neuroprotective in many neurodegenerative disease models. Our studies reveal that SIRT1 also offers protection against polyQexpanded AR by deacetylating the AR at lysines 630/632/633. This finding suggested that nuclear AR acetylation …

Simulating The Time-Dependent Diffusion Coefficient In Mixed-Pore-Size Materials, Zhigang Zhang, David L. Johnson, Lawrence M. Schwartz

Graduate School of Oceanography Faculty Publications

Porous media with a wide distribution of pore sizes are quite common. We show that variable-step-size random walk simulations can be used to model the time-dependent diffusion coefficient D(t) in such porous media. The issue to be overcome is that, in variable-step-size walks, each walker carries its own “clock,” and its position is known only at a random set of times. Thus, a direct ensemble-average calculation of ⟨Δr2(t)⟩ (the mean-square distance traveled at time t) is problematic. We introduce a sequence of approximations that overcome this apparent difficulty. Calculations are carried out on periodic systems that contain pores of quite …

Highway Safety Investigation Manual For The Oregon Department Of Transportation, Karen Dixon, Christopher Monsere

Civil and Environmental Engineering Faculty Publications and Presentations

Across the state, region, and nation highway safety investigators have developed a wide variety of tools and techniques for highway safety investigation procedures. Analysis techniques can range from systematic evaluation approaches such as the Oregon Department of Transportation’s (ODOT’s) Safety Priority Index System (SPIS) down to specific localized safety assessment strategies. It is important to develop consistent methods for safety evaluations across Oregon to ensure that safety investigations are conducted in a similar manner. It is also important to develop a screening process for proper highway safety investigations and document the procedures used for this assessment.

The objective of this …

Confidentiality Explained: The Dialogue Approach To Discussing Confidentiality With Clients, Elisia M. Klinka, Russell G. Pearce

Faculty Scholarship

Many lawyers lie to their clients by saying, “Everything you tell me is confidential” despite the existence of some rules that require lawyers to disclose client confidences and others that permit lawyers to disclose confidences to protect themselves or collect fees. In an effort to encourage clients to speak honestly, a number of commentators urge lawyers to provide either a general or specific notice of the exceptions to confidentiality. This Article offers a different approach. It seeks to promote an honest and open dialogue between lawyer and client that will create a relationship of mutual trust and will make it …

Assessing The Stability Of Elite Political Culture: An Empirical Analysis Of The Attitudes Of Elected Officials In Canada, Lydia Summerlee

Theses and Dissertations (Comprehensive)

Do attitudes of Members of the 32nd and 40th parliament differ on issues of political ethics? Or have attitudes remained unchanged? Based on interviews of 44 members of the 40th parliament and 107 members of the 32nd parliament, this thesis explores attitude shifts across time. The study reveals that in the areas of conflict of interest and patronage attitudes have hardened. Members of Parliament (MPs) from the 40th parliament are more sensitive to these dimensions of political corruption as opposed to MPs from the 32nd parliament. For ethically charged scenarios dealing with gifts & gains and lying, MPs from the …

Linking Cd Accumulation And Effect In Resistant And Sensitive Freshwater Invertebrates, Tony Straus

Theses and Dissertations (Comprehensive)

There has been recent discussion within the field of environmental toxicology as to the viability of using the bioaccumulation of a chemical toxicant to predict toxic effects. Current literature has shown this concept to carry some merit, although there is a major flaw in that with many cases, namely those involving sensitive species, body burdens cannot be reliably linked to effects (Adams et al. (2010)). Bearing this issue in mind the goal of this thesis was to assess the viability of using bioaccumulation as a measure of effect in a different way.

The proposed idea utilized Cd accumulation in a …

A Review Essay: Four Views On Church And Politics, Brian Edgar

The Asbury Journal

Good and Bad Ways to Think about Religion and Politics

Robert Benne

2010. Grand Rapids: Eerdmans

Politics According to the Bible: A Comprehensive Resource for Understanding Modern Political Issues in Light of Scripture

Wayne Grudem

2010. Grand Rapids: Zondervan

Christianity and Politics: A Brief Guide to the History

C.C. Pecknold

2010. Eugene, Oregon: Cascade Books

The Challenge of Pluralism: Church and State in Five Democracies, 2nd ed.

Stephen Monsma and J. Christopher Soper

2009. Lallham, Maryland: Rowman and Littlefield Publishers

Characterization Of Photochromogenic Mycobacterium Spp. From Chesapeake Bay Striped Bass Morone Saxatilis, D. T. Gauthier, A. M. Helenthal, M. W. Rhodes, W. K. Vogelbein, H. I. Kator

Biological Sciences Faculty Publications

A large diversity of Mycobacterium spp. has been isolated from striped bass Morone saxatilis in Chesapeake Bay, USA. The new species M. shottsii and M. pseudoshottsii are the dominant isolates, while the classical fish pathogen M. marinum is found much less frequently. M. fortuitum and M. chelonae, other Mycobacterium spp. known to commonly infect fishes, have not yet been aseptically isolated from striped bass within Chesapeake Bay. While M. pseudoshottsii and M. shottsii have been phenotypically and genotypically characterized, other less common mycobacterial isolates have not. In the present study, we describe 17 photochromogenic isolates from Chesapeake …

Human Endogenous Retrovirus Transcription Profiles Of The Kidney And Kidney-Derived Cell Lines, Sonja Haupt, Michele Tisdale, Michelle Vincendeau, Mary Anne Clements, David T. Gauthier, Raymond Lance, O. John Demmes, Adriana Tuqueti-Neves, Elfriede Noessner, Christine Leib-Mösch, Alex D. Greenwood

Biological Sciences Faculty Publications

The human genome comprises approximately 8-9% of human endogenous retroviruses (HERVs) that are transcribed with tissue specificity. However, relatively few organs have been examined in detail for individual differences in HERV transcription pattern, nor have tissue-to-cell culture comparisons been frequently performed. Using an HERV-specific DNA microarray, a core HERV transcription profile was established for the human kidney comparing 10 tissue samples. This core represents HERV groups expressed uniformly or nearly so in non-tumour kidney tissue. The profiles obtained from non-tumour tissues were compared to 10 renal tumour tissues (renal cell carcinoma, RCC) derived from the same individuals and additionally, to …

Conformation And Ion Transport Of Neuronal Uncoupling Proteins: Ucp2, Ucp4, And Ucp5, Tuan Hoang

Theses and Dissertations (Comprehensive)

Located in the inner mitochondrial membrane of brown adipose tissue, uncoupling protein -1 (UCP1) dissipates the proton electrochemical gradient, causing reduction in the rate of ATP synthesis, and generates heat by non-shivering thermogenesis. Three other UCP homologs (UCP2, UCP4 and UCP5), expressed in neurons, are suggested to have potential roles in the function and protection of the central nervous system (CNS). Up to date, structural information for UCPs still remains limited. Extensive biochemical studies on UCP2 have provided adequate evidence for its participation in proton and anion transport. So far, no functional studies in proteoliposome systems have been performed on …

An Approach To Engagement With Youth In Gangs Participatory Action Projects, Lisa Armstrong

Theses and Dissertations (Comprehensive)

The purpose of this study was to investigate the process of engaging youth who have been involved with gangs in the context of a local street gang prevention project. A case study of the local project was conducted with interviews with twelve (N= 12) participants, most of whom were project staff Interviews were conducted by the author and two youth co-researchers. Findings illustrate the meaning of youth engagement to the participants, ideal roles for staff members and youth, and the relevance of a youth advisory committee as an outlet for future engagement in this program. Based on these …

Examining Experiences Of English Literacy Development (Eld) Program From Multiple Stakeholders’ Perspectives, Hany Ibraham

Theses and Dissertations (Comprehensive)

The present study, conducted in collaboration with a public school board in southern Ontario, Canada, focused on stakeholders’ perspectives of an English Literacy Development (ELD) program offered in secondary schools within this particular board. The purpose of this research was to identify resources that would help address school absenteeism, credit accumulation and graduation needs of ELD learners, as well as to learn which factors in ELD better assist students who are refugees with school integration and in reaching their academic potentials. The research objectives were (1) to identify factors that influence integration of refugee students in school and (n) to …

Monte Carlo Comparison Of Back-Propagation, Conjugate-Gradient, And Finite-Difference Training Algorithms For Multilayer Perceptrons, Stephen Wehry

Theses

Monte Carlo Simulation is used to compare the performance of the Back-Propagation, Conjugate-Gradient, and Finite-Difference algorithms when training simple Multilayer Perceptron networks to solve pattern recognition and bit counting problems. Twelve individual simulations will be run for each training algorithm-test problem combination, resulting in an overall total of 72 simulations. The random elements in each Monte Carlo simulation are to be the individual synaptic weights between layers, which will be uniformly distributed. Two other factors, the size of the hidden layer and the exponent of the error function, will also be tested within the simulation plan outlined above.