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2011

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Inhibition And Nucleic Acid Binding Studies Of The Carboxyltransferase Component Of Bacterial Acetyl-Coa Carboxylase, Brian Benson Jan 2011

Inhibition And Nucleic Acid Binding Studies Of The Carboxyltransferase Component Of Bacterial Acetyl-Coa Carboxylase, Brian Benson

LSU Doctoral Dissertations

Acetyl-CoA carboxylase is an essential enzyme, as it catalyzes the first committed and regulated step in fatty-acid biosynthesis in all organisms excepting few Archaea and Eubacteria. Acetyl-CoA carboxylase from gram-negative and gram-positive bacteria is a multifunctional enzyme composed of three separate proteins. The carboxyltransferase subunit catalyzes the transfer of a carboxyl group from carboxybiotin to acetyl-CoA, forming malonyl-CoA. The crystal structure of the Escherichia coli (E. coli) carboxyltransferase component of acetyl-CoA carboxylase revealed a unique Zn-domain, presumed to mediate nucleic acid binding, that is absent in the eukaryotic enzyme. Notably, the Zn-domain, adjacent to the active site of carboxyltransferase, makes …


Investigating Student Understanding Of The Law Of Conservation Of Matter, Shirley Lynn Tremel Jan 2011

Investigating Student Understanding Of The Law Of Conservation Of Matter, Shirley Lynn Tremel

Dissertations and Theses

The purpose of this study was to gather information about how students learn the foundational concept of conservation of matter during a non-chemistry unit on the rock cycle. The unit covered the rock cycle, rock types, and the law of conservation of matter and took place in a sixth grade classroom of 30 students. A mixed methods, quasi-experimental, pre-post, delayed post design was used to measure student understanding of the concept of conservation of matter as it relates to the rock cycle. Students made significant learning gains from pre-test to post-test and showed mastery in less complex subject areas, but …


Application Of Scanning Probe Microscopy For New Physical Measurements And Studies Of Surface Chemical Reactions Of Materials At The Molecular Level, Stephanie Loletha Daniels Jan 2011

Application Of Scanning Probe Microscopy For New Physical Measurements And Studies Of Surface Chemical Reactions Of Materials At The Molecular Level, Stephanie Loletha Daniels

LSU Doctoral Dissertations

Scanning probe microscopy (SPM) provides unique capabilities for surface visualization and measurements that reach atomic and molecular dimensions. My research focus is directed toward applying and developing new measurements for analytical and surface chemistry with SPM. Two distinct goals based on studies with atomic force microscopy (AFM) will be described within this dissertation. The primary goal was to develop and apply a new AFM imaging mode for ultrasensitive measurements of the superparamagnetic properties of proteins. Magnetic sample modulation (MSM)-AFM, has capabilities to investigate and map the magnetic response of nanomaterials with unprecedented spatial resolution. The second goal was to apply …