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Genetic And Biochemical Studies Of Plasmid Pir52-1 In Lactobacillus Helveticus, Cody Alexander Tramp

Undergraduate Honors Capstone Projects

Lactobacillus helveticus is a species of lactic acid bacteria. These bacteria, which produce lactic acid as a major product of carbohydrate metabolism, are used industrially to produce cheese and other fermented dairy products (Ebringer et al., 2008). Many species of lactic acid bacteria also possess probiotic characteristics and when ingested potentially confer increased immune function, regulate gut microbiota, and improve digestion in the host (Reid, 2008). Genetic studies of these probiotic effects and other characteristics of L. helveticus and related species have been hindered by the lack of stably replicating plasmid vectors. Plasmid vectors for several Lactobacillus species have been …

The Male Gender Role And Depression, Tom Liljegren

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

Although depression is a common mental health disorder, less research has been devoted to men's experience with depression compared to women's experiences. Although men may exhibit similar patterns of depression as women, men often have unique pattern of exhibiting depression characterized by substance abuse, irritability, aggression, and interpersonal conflict. The paper presents a review of the relevant literature on male depression and, in particular, how it is potentially affected by male gender role factors. Biological, psychological, social, and artifact theories have been proposed to explain gender differences in how depression is expressed. It is hypothesized that the male gender role …

Design And Scale-Up Of Production Scale Stirred Tank Fermentors, Ryan Z. Davis

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In the bio/pharmaceutical industry, fermentation is extremely important in pharmaceutical development, and in microbial research. However, new fermentor designs are needed to improve production and reduce costs of complex systems such as cultivation of mammalian cells and genetically engineered micro-organisms. Traditionally, stirred tank design is driven by the oxygen transfer capability needed to achieve cell growth. However, design methodologies available for stirred tank fermentors are insufficient and many times contain errors. The aim of this research is to improve the design of production scale stirred tank fermentors through the development of dimensionless correlations and by providing information on aspects of …

Neural Networks And The Natural Gradient, Michael R. Bastian

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Neural network training algorithms have always suffered from the problem of local minima. The advent of natural gradient algorithms promised to overcome this shortcoming by finding better local minima. However, they require additional training parameters and computational overhead. By using a new formulation for the natural gradient, an algorithm is described that uses less memory and processing time than previous algorithms with comparable performance.

Does Empathic Accuracy Mediate The Relationships Between Individual Psychological Characteristics And Adolescent Romantic Relationship Functioning?, Charles George Bentley

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study investigated empathic accuracy in adolescent romantic relationships. The project examined the relationships between psychological characteristics and relationship outcomes (i.e., satisfaction and aggression) to determine if the relationships were mediated by empathic accuracy. Participants were 92 heterosexual couples aged 14-18 years old who lived in rural areas in Utah and Arizona. Couple members completed surveys assessing attitudes and behaviors in their relationships and a video-recall procedure in which partners rated their own and their partner's behaviors during problem-solving discussion.

Empathic accuracy was generally not related to psychological characteristics or outcomes. It became apparent that there were limitations with the …

Comparison Of Agricultural Area Source Ammonia Gas Concentration And Flux Measurements, Emyrei Reese

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study was conducted in order to compare and evaluate a variety of ammonia (NH₃) measurement techniques for determination of ambient gas phase concentration and evaluation of system fluxes. This was accomplished by measuring NH3 emitted from area sources within two dairies, one located in south-central Idaho and another in central California. The measurement techniques utilized included: an array of passive diffusion samplers, a single annular denuder system, a single or paired Fourier transform infrared spectrometer(s) (FTIR), a paired (upwind and downwind) set of differential optical absorption spectrometers (DOAS), and a portable wind tunnel in conjunction with a …

Drivers Of Plant Population Dynamics In Three Arid To Subhumid Ecosystems, Luke J. Zachmann

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Understanding the relative importance of density-dependent and density-independent factors in driving population dynamics is one of the oldest challenges in ecology, and may play a critical role in predicting the effects of climate change on populations. We used long-term observational data to describe patterns in plant population regulation for 57 forb and grass species from three different ecosystems (arid desert grassland, semiarid sagebrush steppe, and subhumid mixed-grass prairie). Using a hierarchical partitioning approach, we (i) quantified the relative influence of conspecific density, heterospecific composition, and climate on temporal variation in population growth rates, and (ii) asked how the relative importance …

Effects Of Professional Development On Infusing Engineering Design Into High School Science, Technology, Engineering, And Math (Stem) Curricula, Zanj Kano Avery

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to examine the effects of professional development (PD) on the infusion of engineering design into high school curricula. Four inservice teachers with backgrounds in physics, chemistry, industrial education, math, and electrical engineering participated in the 2006 National Center of Engineering and Technology Education (NCETE)-sponsored PD workshops at California State University, Los Angeles (CSULA) and provided three sources of data that were used to answer the research question, "What are the effects of PD on infusing engineering design into high school science, technology, engineering, and math (STEM) curricula"?

Three major themes emerged from the data. …

Fundamentals Of Operation Of The Induced Bed Reactor (Ibr) Anaerobic Digester, Jacob Shaun Dustin

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Induced Bed Reactor (IBR) was developed at Utah State University to apply high-rate anaerobic digestion techniques to high solids content substrates. This technology has been successfully implemented at full-scale multiple installations in the United States and Canada as a waste treatment and energy production technology, but the physical processes necessary to further optimize the system were not well understood.

Bench scale IBRs were operated as anaerobic digesters at 35°, 45°, and 55° C under three organic loading rates and three corresponding hydraulic retention times. Reactor performance was monitored at steady state for residence time distribution and substrate reduction.

The …

Introduced Sport Fish And Fish Conservation In A Novel Food Web: Evidence Of Predatory Impact, Kevin Lee Landom

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study addressed a fundamental question in applied ecology and conservation; what is the predatory impact of introduced piscivorous sport fish on imperiled native fish populations? More specifically, which of many introduced species and size-classes represent the greatest threats and should be targeted for control? In order to explore this important question, an integrated analysis of stable isotopes, quantified observed diet analysis, and stable isotope mass-balance models were used to quantify trophic interactions. These tools were used to construct food web models that were then compared to draw inferences regarding the relative contribution of prey fish, including rare native fish, …

Learning Ability And Factors Influencing Nest Establishment Of The Solitary Bees Osmia Lignaria And Megachile Rotundata (Hymenoptera: Megachilidae), Cory A. Vorel

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Over the last several decades, the use of solitary bees as an alternative to honey bees (Apis mellifera L.) for pollination of commercial crops has increased, in part as a response to ongoing problems faced by commercial honey bee populations. Two solitary bee species have exhibited great commercial potential: the blue orchard bee, Osmia lignaria Say, and the alfalfa leafcutting bee, Megachile rotundata Fabricius (Hymenoptera: Megachilidae). However, growth of O. lignaria and M. rotundata populations is limited in commercial systems, mainly due to low establishment of females at provided nesting sites, possibly due to mortality, dispersal, or other causes. …

Characterizing Ecologically Relevant Variations In Streamflow Regimes, Kiran J. Chinnayakanahalli

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Maintaining the ecological health of streams is vital for sustainable water resources management. Streamflow is a primary factor influencing the structure and function of ecological communities. A quantitative understanding of how stream biota respond to variation in streamflow is required for stream bioassessment. This dissertation focuses on quantifying relationships between streamflow regime and stream macroinvertebrate richness and composition. The contribution comprises statistical models that predict stream macroinvertebrate class from streamflow regime and predict streamflow regime from watershed attributes, and a tool that helps derive watershed attribute variables used in these models.

The dissertation is a collection of three papers. In …

The Ecological Influences Of Community Trail Use And Physical Activity Among Female Trail Users, Sarah N. Moulton

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Despite great effort to improve physical activity, little success has come to pass. Due to this, some researchers have shifted from individual approaches to promoting physical activity to multilevel environmental approaches. One of these multifaceted environmental approaches is through community trails. A small body of research has suggested that community trails may be a successful ecological approach to promoting physical activity. However, the research is minimal and inconsistent in supporting effectiveness of community trails for promoting physical activity.

This study sought to examine ecological factors to learn if and how they might influence trail use among women. The ecological factors …

Data Collection And Analysis Methods For Two-Zone Temperature And Solute Model Parameter Estimation And Corroboration, Quinten Glen Bingham

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Water temperature directly affects biological and chemical processes of fresh water ecosystems. Elevated instream temperatures are commonplace in the Virgin River of southwestern Utah during summer due to a hot desert climate and high water demands that result in low stream flows. This is of concern since the Virgin River is home to two endangered species, the Virgin River Chub (Gila seminuda) and Woundfin (Plagopterus argentissimus). Efforts to model instream temperatures within the Virgin River have been undertaken to help mitigate elevated instream temperatures including the development of a two-zone temperature and solute (TZTS) model. This …

Optimizing The Physical And Nutritional Environment Of Unleached Root-Zones, Curtis B. Adams

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Unleached root-zones represent an environmental ideal by eliminating wasteful leaching of nutrients and water. NASA grows plants in space in unleached root-zones, incorporating polymer-coated fertilizer (PCF) into a ceramic media (Turface or Profile). However, lack of growth productivity in space has led to the need for research to improve the nutritional and physical environment of the root-zone, which is the objective of this research. PCF types are diverse in release characteristics and the effects of temperature and substrate water content have not been well characterized. In spite of widespread use, studies on chemical properties and applied studies to verify soil …

Evaluation Of Semiochemical Strategies For The Protection Of Whitebark Pine Stands Against Mountain Pine Beetle Attack Within The Greater Yellowstone Ecosystem, Greta Katherine Schen-Langenheim

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

High-dose verbenone, verbenone plus nonhost volatiles (NHVs), and both semiochemicals in combination with aggregant-baited funnel traps were tested for stand- level protection against mountain pine beetle attack for two consecutive years (2004-2005) at three seral high elevation whitebark pine sites in the Greater Yellowstone Ecosystem. In 2004, two 0.25-hectare treatments comprised of 25 high-dose verbenone pouches or verbenone pouches combined with single baited funnel traps were tested in a push-pull strategy. In 2005, 25 high-dose verbenone and 25 NHV pouches, or verbenone and NHV in combination with baited funnel trap clusters were tested. In both years, treatments were compared to …

Thioester Hydrolysis Reactivity Of Metal Complexes, James Justin Danford

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Glyoxalase II is one of two metalloenzymes found in the glyoxalase pathway and is responsible for catalyzing the hydrolysis of a thioester substrate. Its bimetallic active site is found to contain a variety of metal combinations, including Fe(III)Zn(II). A recent report indicates that human glyoxalase II, while containing a Fe(II)Zn(II) center, is catalytically active as a mononuclear Zn(II) enzyme. Detailed mechanistic studies of glyoxalase II enzymes are limited due to uncertainty in the metal ion content of recombinantly prepared samples. The research presented in this thesis is focused on gaining mechanistic insight into thioester hydrolysis promoted by well-characterized metal complexes …

Mechanisms Of The Extreme Sensitivity Of Turkeys To Aflatoxin B1, Sumit Rawal

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The pathogenesis of hepatotoxic and hepatocarcinogenic actions of the mycotoxin aflatoxin B1 (AFB1) involves initial bioactivation by microsomal cytochrome P450s (P450) to a reactive and electrophilic intermediate, exo-aflatoxin B1-8,9-epoxide (exo-AFBO). Poultry, especially turkeys, are extremely sensitive to AFB1, a condition associated with efficient epoxidation by P450s. The purpose of this research was to 1) discover and characterize the P450s in turkey liver responsible for AFB1 bioactivation, and 2) determine the relative importance of these P450s in turkey liver.

Initial investigations led to the discovery of CYP1A5. We then identified CYP3A37, a human CYP3A4 homologue from turkey liver, …

Physiological And Molecular Function Of Hap3b In Flowering Time Regulation And Cold Stress Response, Mingxiang Liang

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Heme-activated proteins (HAPs) are transcription factors that have multiple roles in plant growth and development, such as embryogenesis, flowering time control, and drought tolerance.

In the present study I found that HAP3b was also involved in controlling response to cold stress. Transcript profiling and gene expression analyses indicated that HAP3b repressed the CBF3 regulon under normal growth conditions. As a result, plants with HAP3b-overexpressed showed decreased survival rates while plants homozygous for the null allele hap3b showed an improved freezing tolerance compared to wild-type plants.

To understand the mechanism of HAP3b in Arabidopsis, i.e. whether it also acts …

Characterization And Physicochemical Modifications Of Polymer Hollow Fiber Membranes For Biomedical And Bioprocessing Applications, Benjamin R. Madsen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Hollow fiber membranes (HFMs) formed through phase inversion methods exhibit specific physicochemical characteristics and generally favorable surface and mechanical properties, supporting their use in diverse applications including ultrafiltration, dialysis, cell culture, bioreactors, and tissue engineering. Characterization of, and modifications to, such membranes are important steps in achieving desired characteristics for specific applications.

HFMs subject to gas, irradiation, and chemical sterilization techniques were characterized based on several analytical techniques. It was revealed that these common sterilization techniques can cause inadvertent changes to HFM properties. While these changes may cause detrimental effects to HFMs used in filtration, the methods of sterilization are …

Snowshoe Hare And Forest Structure Relationships In Western Wyoming, Nathan Daniel Berg

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Snowshoe hares (Lepus americanus) are a critically important prey species for Canada lynx (Lynx canadensis). Determination of snowshoe hare distribution and abundance is needed in western Wyoming for lynx conservation. We used linear regression to examine the correlations between snowshoe hare density, as determined by mark-recapture estimates, and fecal pellet plot counts on both uncleared and annually cleared plots on the Bridger-Teton National Forest, western Wyoming. We found significant correlations between hare density estimates and fecal pellet counts for both uncleared and annually cleared pellet counts; however the relationship was much stronger for annually cleared pellet …

Internal Design Of Mechanically Stabilized Earth (Mse) Retaining Walls Using Crimped Bars, Bernardo A. Castellanos

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Current design codes of Mechanically Stabilized Earth (MSE) Walls allow the use lower lateral earth pressure coefficient (K value) for designing geosynthetics walls than those used to design steel walls. The reason of this is because geosynthetics walls are less rigid permitting the wall to deform enough to work under active pressures instead of at rest pressures as in steel walls.

A new concept of crimped steel bars was recently introduced. This new type of bar was tested for tension and pullout behavior. Results on tests made on crimped bars show that putting those crimps in the steel bar will …

Assessing Quaking Aspen (Populus Tremuloides) Decline On Cedar Mountain In Southern Utah Using Remote Sensing And Geographic Information Systems, Chad M. Oukrop

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Quaking aspen (Populus tremuloides Michx.) is the most widespread deciduous tree species in North America and aspen ecosystems are highly valued for multiple use, being noted for forage production, understory diversity, wildlife habitat, timber, hydrological assets, and aesthetics. However, aspen communities in the Intermountain Region of the western United States are in evident decline, with certain areas experiencing rapid mortality over the past decade. One location of special interest is the quaking aspen on Cedar Mountain in Southern Utah, USA, an isolated population in the southwestern portion of aspen's geographic range.

Lacking critical information on the location, extent, and …

Host Signaling Response To Adhesion Of Bifidobacterium Infantis, Reed N. Gann

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Investigations of the molecular binding partners of the probiotic bacterium Bifidobacterium longum subspecies infantis (B. infantis) and the pathogen Salmonella enterica subspecies enterica serovar Typhimurium LT2 (Salmonella ser. Typhimurium) found that these two very different bacteria bind gangliosides. However, these organisms lead to completely different host health outcomes when present in the gut. B. infantis is the founding microbial population in the intestinal tract of breast-fed infants. S. typhimurium is the most important food-borne pathogen that results in humans. This study used an in vitro gut epithelial cell model to examine the host cellular response to adhesion …

Common Characteristics Of Young People Who Text: The Connection To Autonomy, Identity And Self-Esteem, Elizabeth M. Davis

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study examined the relationship between adolescents' use of texting and their level of identity development, cognitive autonomy, and self-esteem. One hundred and nineteen Utah State University students participated in this study by filling out a questionnaire that included a section designed specifically to learn the texting habits of the participants, and also included sections derived from previously existing measures on identity, autonomy, and self-esteem.

The results show statistical significance of age and texting. Participants who were 23 years+ sent significantly less than those participants who were 19-22 years. Areas of gender, identity, and self-esteem were also analyzed. Implications of …

Analysis Of Ammonia And Volatile Organic Amine Emissions In A Confined Poultry Facility, Hanh Hong Thi Dinh

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The National Air Emission Monitoring Study (NAEMS) project was funded by the Agricultural Air Research Council (AARC) to evaluate agricultural emissions nationwide. Utah State University (USU) is conducting a parallel study on agricultural emissions at a Cache Valley poultry facility. As part of this parallel study, samples of animal feed, eggs and animal waste were collected weekly from three manure barns (designated: manure barn, barn 4 - manure belt and barn 5 - high rise) from May 2008 to November 2009.

These samples were analyzed to determine ammonia content, total Kjeldahl nitrogen content and ammonia emission. The yearly average calculated …

Development Of A Laboratory Based System For Selecting Insect Pathogenic Fungi With Greatest Potential For Success In The Field, Chad Alton Keyser

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Many insects are important agricultural pests, and active control is necessary to keep them at abeyance. The naturally occurring entomopathogenic fungus Metarhizium is a promising tool to control pest insects, and its use avoids the well-known harmful side effects of chemical pesticides. Thousands of unique isolates of Metarhizium exist throughout the world. These isolates vary widely in their ability to cause infection and to tolerate stressful habitats. The research reported here tests the THESIS: A laboratory-based system can be devised that identifies, from among many Metarhizium isolates, those isolates with the greatest potential for successful biological control of pest insects …

Kindergarten Teachers' Developmentally Appropriate Beliefs And Practices And Their Perceptions Of Children's Kindergarten Readiness: Comparing The Beginning And The End Of The School Year, Rachelle Ernest Wright

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study examined 180 matched pretest/posttest surveys of kindergarten teachers' perceptions of the transition children experienced upon kindergarten entry. Investigations of changes in the percentages of children perceived as not being ready for kindergarten and percentages of children who were rated as having had a very successful, moderately successful, or difficult transition from the pretest to the posttest were conducted. Additionally, changes in teachers' developmentally appropriate beliefs and practices from the beginning of the school year (pretest) to the end of the school year (posttest) were explored. Further analyses were conducted to find differences and associations between teacher and classroom …

Quantifying Legacy Effects Of Managed Disturbance On Sagebrush Steppe Resilience And Diversity, Julie Ripplinger

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Land-use legacies can affect landscapes for decades to millennia. A long history of shrub management exists in the sagebrush steppe of the Intermountain West where shrub-removal treatments, a type of managed disturbance, have been implemented for over 50 years to reduce sagebrush cover. The assumption behind managed disturbances is that they will increase forage for domestic livestock and improve wildlife habitat. However, the long-term effects of managed disturbance on plant community composition and diversity are not well understood. We investigated the legacy effects of three common types of managed disturbance (chemical, fire, and mechanical treatments) on plant community diversity and …

Development Of A Discrete Mathematics Textbook And Guide For High School Teachers, Rebecca A. Stokes

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

This project was to create the beginnings a textbook for teacher's to supplement instruction of a discrete mathematics course at the high school level. The development of the text was guided by past and current efforts to place discrete mathematics in high school curriculum. A review of the literature and experiences of instructors were viewed and analyzed to guide the construction of the textbook. The text was written with the goal to give teachers information about the topics of discrete mathematics, extra resources, lesson ideas , and optional worksheets for students. Several lessons were created and one was implemented in …