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Rapid Microsatellite Isolation From A Butterfly By De Novo Transcriptome Sequencing: Performance And A Comparison With Aflp-Derived Distances, As Mikheyev, T Vo, B Wee, Mc Singer, C Parmesan

School of Biological and Marine Sciences

BACKGROUND: The isolation of microsatellite markers remains laborious and expensive. For some taxa, such as Lepidoptera, development of microsatellite markers has been particularly difficult, as many markers appear to be located in repetitive DNA and have nearly identical flanking regions. We attempted to circumvent this problem by bioinformatic mining of microsatellite sequences from a de novo-sequenced transcriptome of a butterfly (Euphydryas editha). PRINCIPAL FINDINGS: By searching the assembled sequence data for perfect microsatellite repeats we found 10 polymorphic loci. Although, like many expressed sequence tag-derived microsatellites, our markers show strong deviations from Hardy-Weinberg equilibrium in many populations, and, in some …

Refuge Update Article Index

RefugeUpdate (USFWS-NWRS)

Use the “Find” function on your browser to search the index by topic, refuge name or article author.

Vs - Bovine Tuberculosis (Mycobacterium Bovis) Surveillance Standards 11/2001

Michigan Bovine Tuberculosis Bibliography and Database

Bovine tuberculosis (TB) is an infectious and communicable granulomatous disease caused by the acidfast bacilli bacteria of Mycobacterium bovis (M. bovis). It is commonly a chronic, debilitating disease, but occasionally may assume an acute, rapidly progressive course. M. bovisis a widespread zoonosis that is global in magnitude and affects nearly all species of vertebrates (cattle, sheep, goats, pigs, bison, buffalo, and camelids.) Disease is spread by direct contact, inhalation of infected droplets expelled from infected lungs, and ingestion of contaminated feed or milk. In most countries, TB is a notifiable disease. Overall, TB has an important world-wide …

Plant Neighbor Identity Influences Plant Biochemistry And Physiology Related To Defense, Amanda K. Broz, Corey D. Broeckling, Clelia De-La-Peña, Matthew R. Lewis, Erick Greene, Ragan M. Callaway, Lloyd W. Sumner, Jorge M. Vivanco

Biological Sciences Faculty Publications

Background: Chemical and biological processes dictate an individual organism's ability to recognize and respond to other organisms. A small but growing body of evidence suggests that plants may be capable of recognizing and responding to neighboring plants in a species specific fashion. Here we tested whether or not individuals of the invasive exotic weed, Centaurea maculosa, would modulate their defensive strategy in response to different plant neighbors. Results: In the greenhouse, C. maculosa individuals were paired with either conspecific (C. maculosa) or heterospecific (Festuca idahoensis) plant neighbors and elicited with the plant defense signaling molecule methyl jasmonate to mimic insect …

Optical Measurement Of Environmental Uranium Using Porous Silica Materials, Chien-Cheng Chen

Theses and Dissertations

The focus of this research is on the optical measurement of uranyl in a solid matrix using fluorescence spectroscopy. Nanoporous silica-based materials were used to extract uranyl from contaminated soil and to enhance the fluorescence intensity and lifetime. The fluorescence lifetime and intensity of uranyl ions adsorbed on porous silica-based materials of varying pore size was measured as a function of pH and in the presence of fluoride. The feasibility of uranyl fluorescence detection on the top of soil by silica gel is carried out by four types of natural soil. The results show that the uranyl fluorescence intensity can …

Interview No. 1482, Carlos Guzman

Combined Interviews

Carlos Guzman was born and raised in Puerto Rico and lived in there until he joined the United States Army. Prior to joining the military, Carlos obtained his Bachelor degree from the University of Puerto Rico in biology and chemistry. While in the military, Carlos served as an officer and served in Iraq from 2004 until 2005. Upon returning from Iraq, Carlos began to pursue his M.A. in computer resource management. It was during this time that Carlos met Larry Walter and the two became interested in biofuel technology after a discussion on biofuel. Carlos began investigating what it would …

Mission Assurance: A Review Of Continuity Of Operations Guidance For Application To Cyber Incident Mission Impact Assessment (Cimia), Brian L. Hale

Theses and Dissertations

Military organizations have embedded information technology (IT) into their core mission processes as a means to increase operational efficiency, improve decision-making quality, and shorten the sensor-to-shooter cycle. This IT-to-mission dependence can place the organizational mission at risk when an information incident (e.g., the loss or manipulation of a critical information resource) occurs. Non-military organizations typically address this type of IT risk through an introspective, enterprise-wide focused risk management program that continuously identifies, prioritizes, and documents risks so an economical set of control measures (e.g., people, processes, technology) can be selected to mitigate the risks to an acceptable level. The explicit …

Interview No. 1482, Carlos Guzman

Paso del Norte Entrepreneurship Oral History Project

Carlos Guzman was born and raised in Puerto Rico and lived in there until he joined the United States Army. Prior to joining the military, Carlos obtained his Bachelor degree from the University of Puerto Rico in biology and chemistry. While in the military, Carlos served as an officer and served in Iraq from 2004 until 2005. Upon returning from Iraq, Carlos began to pursue his M.A. in computer resource management. It was during this time that Carlos met Larry Walter and the two became interested in biofuel technology after a discussion on biofuel. Carlos began investigating what it would …

The Impact Of Prior Sexual Victimization And Victim Identification On Threat Recognition In A College Sample, Katherine Porter

Master's Theses and Doctoral Dissertations

Previous research on sexual assault has demonstrated a pattern of revictimization, in which victims of childhood sexual assault are at an increased risk for adult sexual assault (e.g., Tjaden & Thoennes, 2000). Previous findings indicate that participants with a history of sexual assault take longer to identify and respond to risks (Marx et al., 2001; Soler-Baillo et al., 2005; Wilson et al., 1999), are less likely to respond in assertive ways (VanZile-Tamsen et al., 2005), and are more likely to indicate that they would be compliant in risky sexual situations (Naugle, 1999).

The present study attempted to replicate and expand …

Alternative Trans-Splicing Of Caenorhabditis Elegans Sma-9/Schnurri Generates A Short Transcript That Provides Tissue-Specific Function In Bmp Signaling, Jianghua Yin, Ling Yu, Cathy Savage-Dunn

Publications and Research

Background Transcription cofactors related to Drosophila Schnurri facilitate the transcriptional programs regulated by BMP signaling in C. elegans, Drosophila, Xenopus, and mouse. In different systems, Schnurri homologs have been shown to act as either agonists or antagonists of Smad function, and as either positive or negative regulators of transcription. How Schnurri proteins achieve this diversity of activities is not clear. The C. elegans sma-9/schnurri locus undergoes alternative splicing, including an unusual trans-splicing event that could generate two non-overlapping shorter transcripts.

Results We demonstrate here that the shorter transcripts are expressed in vivo. Furthermore, we find that one of the short …

Architectural Considerations For Single Operator Management Of Multiple Unmanned Aerial Vehicles, Gabriel T. Bugajski

Theses and Dissertations

Recently, small Unmanned Aircraft Systems (UAS) have become ubiquitous in military battlefield operations due to their intelligence collection capabilities. However, these unmanned systems consistently demonstrate limitations and shortfalls with respect to size, weight, range, line of sight and information management. The United States Air Force Unmanned Aircraft Systems Flight Plan 2009-2047 describes an action plan for improved UAS employment which calls out single operator, multi-vehicle mission configurations. This thesis analyzes the information architecture using future concepts of operations, such as biologically-inspired flocking mechanisms. The analysis and empirical results present insight into the engineering of single-operator multiple-vehicle architectures.

Use Of Negation In Search, Kristen M. Lancaster

Theses and Dissertations

Boolean algebra was developed in the 1840s. Since that time, negation, one of the three basic concepts in Boolean algebra, has influenced the fields of information science and information retrieval, particularly in the modern computer era. In Web search engines, one of the present manifestations of information retrieval, little use is being made of this functionality and so little attention is given to it in the literature. This study aims to bolster the understanding of the use and usefulness of negation. Specifically, an Internet search task was developed for which negation was the most appropriate search strategy. This search task …

Días Inolvidables (Cápitulo 1-1) [9th-12th Grade], Carol Arnold

Understanding by Design: Complete Collection

This unit was designed utilizing the Realidades 3 Textbook from Prentice Hall for a Spanish 3 level class. I also included some differentiation for Spanish 3 Pre-AP along with supplemental activities at the end of the unit. The goal of the unit was to have students investigate in an authentic manner the positive and negative effects of tourism on Spanish-speaking countries.

Throughout their investigations, students are exposed to and expected to use the preterite and imperfect tense in a real-world context through camping and tourism. One of the other goals of the unit is to have students create a presentation …

Regulatory Mechanism Of Myeloid Derived Suppressor Cell Activity, Cesar Alexander Corzo

USF Tampa Graduate Theses and Dissertations

Myeloid-derived suppressor cells (MDSC) are a major component of the immune suppressive network that develops during cancer. MDSC down-regulate immune surveillance and antitumor immunity and facilitate tumor growth. The ability of MDSC to suppress T cell responses has been documented; however the mechanisms regulating this suppression remain to be understood. This work proposes a biological dichotomy of MDSC regulated by the tumor microenvironment. In peripheral lymphoid organs MDSC cause T-cell non-responsiveness that is antigen-specific. These MDSC have increased expression of NOX2, enabling them to produce large amounts of reactive oxygen species. Since the transcription factor STAT3 is substantially activated in …

Organellar Dna Polymerases Gamma I And Ii In Arabidopsis Thaliana, Jeffrey M. Brammer

Theses and Dissertations

Plants have two organelles outside the nucleus which carry their own DNA, mitochondria and chloroplasts. These organelles are descendants of bacteria that were engulfed by their host according to the endosymbiotic theory. Over time, DNA has been exchanged between these organelles and the nucleus. Two polymerases, DNA Polymerases Gamma I and II, are encoded in the nucleus and remain under nuclear control, but are transported into the mitochondria and chloroplasts. DNA polymerases gamma I and II are two organelle polymerases which have been studied through sequence analysis and shown to localize to both mitochondria and chloroplasts. Little has been done …

Thermodynamics Of The Dna Structural Selectivity Of The Pol I Dna Polymerases From Escherichia Coli And Thermus Aquaticus, Andy J. Wowor, Kausiki Datta, Hiromi S. Brown, Gregory S. Thompson, Sreerupa Ray, Anne Grove, Vince J. Licata

Faculty Publications

Understanding the thermodynamics of substrate selection by DNA polymerase I is important for characterizing the balance between replication and repair for this enzyme in vivo. Due to their sequence and structural similarities, Klenow and Klentaq, the large fragments of the Pol I DNA polymerases from Escherichia coll and Thermus aquaticus, are considered functional homologs. Klentaq, however, does not have a functional proofreading site. Examination of the DNA binding thermodynamics of Klenow and Klentaq to different DNA structures: single-stranded DNA (ss-DNA), primer-template DNA (pt-DNA), and blunt-end double-stranded DNA (ds-DNA) show that the binding selectivity pattern is similar when examined across a …

The Bg News June 16, 2010, Bowling Green State University

BG News (Student Newspaper)

The BGSU campus student newspaper. June 16, 2010. Volume 100-Issue 154.

Morris Faculty And Graduates Serve On Advisory Committee For University Of Minnesota President Search, University Relations

Campus News Archive

Professor Nancy Carpenter, Etty DeVeaux ’86, and Carol Person ’73 are honored to help identify the next University president.

A Multimodal Assessment Of The Genetic Control Over Working Memory, Katherine H. Karlsgodt, Peter Kochunov, Anderson M. Winkler, Angela R. Laird, Laura Almasy, Ravi Duggirala, Rene L. Olvera, Peter T. Fox, John Blangero, David C. Glahn

School of Medicine Publications

Working memory performance is significantly influenced by genetic factors. Here, we assessed genetic contributions to both working memory performance and neuroimaging measures focused on the network of brain regions associated with working memory by using a sample of 467 human participants from extended families. Imaging measures included diffusion tensor imaging indices in major white matter tracts thought to be associated with working memory and structural magnetic resonance imaging measures of frontal and parietal gray matter density. Analyses directly addressed whether working memory performance and neural structural integrity are influenced by common genetic factors (e.g., pleiotropy). While all cognitive measures, gray …

Some Recent French Books On Philosophy.

The Open Court

No abstract provided.

A New History Of Modern Europe., Poultney Bigelow

The Open Court

No abstract provided.

Open Inspiration Versus A Closed Canon And Infallible Bible., Charles W. Pearson

The Open Court

No abstract provided.

Book Reviews And Notes.

The Open Court

No abstract provided.

Haeckel's Work On The Artistic Forms Of Nature.

The Open Court

No abstract provided.

A Call To Arms, Mark Y. Herring

Dacus Library Faculty Publications

This month I’m using my column to issue a call to arms. No, it isn’t a call to arms for war, though it is going to be battle. It is a call to professional librarians who are interested in their jobs lasting more than a few more years. That sounds a bit hysterical but I don’t mean for it to. Yet is it hyperbolic? I don’t think so. We need to rethink, recast, redefine, and refresh our professional métier. I think the last twenty-four months make it imperative that we do so now

Holy Cross Libraries Annual Report Fy2010, Karen Reilly

Holy Cross Libraries Annual Reports

At the end of the fiscal year, the Director of the Holy Cross Libraries issues an annual report that provides a review of library services, details budgets and regular operations, and highlights programs, projects and activities that were initiated, sponsored or assisted by the Libraries.

Purification And Characterization Of A Cellulase From Bacillus Subtilis Yj1, Li-Jung Yin, Hsin-Hung Lin, Zheng-Rong Xiao

Journal of Marine Science and Technology–Taiwan

Broth incubated with Bacillus subtilis YJ1 at 37°C for 36 h was collected and removed bacterial cells by passing through a 0.45 µm membrane. A cellulase was purified to electrophoretical homogeneity by ammonium sulfate precipitation, Macro-Prep ion exchange and Bio-Gel P-100 chromatography. The recovery and purification fold were 9.7% and 289, respectively, after Bio-Gel P-100 chromatograph. The purified cellulase, with a molecular mass (M) of 32.5 kDa, had an optimal pH and temperature at 6.0 and 50-60°C, respectively. It was stable at pH 6.0-7.5 and

Ribosome Recycling Step In Yeast Cytoplasmic Protein Synthesis Is Catalyzed By Eef3 And Atp., Shinya Kurata, Klaus H Nielsen, Sarah F Mitchell, Jon R Lorsch, Akira Kaji, Hideko Kaji

Department of Biochemistry and Molecular Biology Faculty Papers

After each round of protein biosynthesis, the posttermination complex (PoTC) consisting of a ribosome, mRNA, and tRNA must be disassembled into its components for a new round of translation. Here, we show that a Saccharomyces cerevisiae model PoTC was disassembled by ATP and eukaryotic elongation factor 3 (eEF3). GTP or ITP functioned with less efficiency and adenosine 5gamma'-(beta,gamma-imido)triphosphate did not function at all. The k(cat) of eEF3 was 1.12 min(-1), which is comparable to that of the in vitro initiation step. The disassembly reaction was inhibited by aminoglycosides and cycloheximide. The subunits formed from the yeast model PoTC remained separated …

Eng 3702-051: American Literature: Mid-19th Century To 1900, John Allison

Summer 2010

No abstract provided.

Insecticide Use And Crop Selection: A South Dakota Case Study, Tia Mcdonald, Ariel Keating, Scott Fausti, Jing Li, Mike Catangui

Economics Staff Paper Series (1969 - 2017)

South Dakota has recently experienced a significant increase in the proportion of acres treated with insecticide. Unfortunately, data on insecticide usage by crop at the county level is not available. The following case study seeks to uncover the reasons for this increase by analyzing county-level data in South Dakota with a fixed effects panel regression. The study links the proportion of acres planted for a specific crop to the proportion of total acres treated with insecticide. This approach provides insight on how changing cropping patterns in South Dakota have influenced insecticide use.