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“Everyday Symbols For Mediation” Conflict And Cooperation Over The Management Of Cultural And Natural Resources Within The Big South Fork National River And Recreation Area, Carol Jo Evans

University of Kentucky Doctoral Dissertations

Utilizing quantitative and qualitative methods, this in-depth ethnographic study of the Big South Fork National River and Recreation Area (BSFNRRA) examines social conflict and resistance stemming from competing values, definitions, and concerns over the management of cultural and natural resources within the region. The timing of this project is fortuitous for the National Park Service (NPS) has completed the creation of a ten year General Management Plan. Thus, we are provided with an opportunity to study and analyze the policy and methodology that park officials are required to follow in creating a management plan and eliciting public participation.

The first …

The Role Of Abcg2 In Drug Active Transport In Milk, Lipeng Wang

University of Kentucky Doctoral Dissertations

Drug active transport into milk is a major concern for breastfeeding mothers and healthcare providers. Studies from the literature indicated that breast cancer resistance protein (ABCG2) plays an important role in drug transfer into milk. There has been limited study on stereoselective interactions with ABCG2. A mechanistic analysis of flux across cell monolayer model is a critical first step toward extrapolating in vitro results for predicting in vivo disposition (including distribution into milk), drug disposition or drug-drug interactions.

The objectives of this thesis were (1) to establish a “Chemical knockout model” in rat for studying drug accumulation into milk, (2) …

Function Of Androgen Receptor In Prostate Cancer Epithelial Mesenchymal Transition And Microtubule Targeting, Menglei Zhu

University of Kentucky Doctoral Dissertations

Prostate cancer is the most frequently diagnosed non-skin cancer and the third leading cause of cancer mortality among men in the US. Androgens are functionally required for the normal growth of the prostate gland and play a critical role in prostate tumor development and progression. Epithelial-mesenchymal-transition (EMT) is an important process during normal development, and cancer cell metastasis.

This study examined the ability of androgens to influence EMT of prostate cancer epithelial cells and evaluate the effect of taxol chemotherapy on androgen signaling in prostate cancer cells in prostate cancer. The EMT pattern was evaluated on the basis of expression …

Improvement Of New Oil Crops For Kentucky, Watchareewan Jamboonsri

University of Kentucky Doctoral Dissertations

Three oil crops, chia (Salvia hispanica L.), flax (Linum usitatissimum L.), and castor (Ricinus communis L.), were studied because of their nutritional and industrial values. Chia and flax are rich in an ω3 fatty acid, α-linolenic acid, and castor is a very high oil producer and high in a hydroxy fatty acid. Ethyl methanesulfonate (EMS) and gamma rays were employed to mutagenize chia seeds to produce early flowering mutants. The M₁ population was grown and induced to flower by short-day photoperiods. The M₂ population was planted in the field in Lexington, KY in 2008. Early …

Testing Lesniaski’S Revised Brief Test, M. Sara Lowe, Sean M. Stone

Library Staff Publications and Research

In 2004, Lesniaski revised White’s Brief Test methodology for smaller academic libraries that lack sufficient subject specialist librarians to perform the brief tests as originally proposed byWhite. As a part of regular collection development, Cowles Library implemented Lesniaski’s Brief Tests on the print monograph collection. While primarily a review of Lesniaski’s methodology, this article also discusses how the Brief Tests were used for improved collection evaluation and maintenance.

Does The Experimental-Use Defense To Patent Infringement Still Exist, Sonya J. Bible

SMU Science and Technology Law Review

No abstract provided.

The Advent Of Gene Patenting: Putting The Great Debate In Perspective, Brian Zadorozny

SMU Science and Technology Law Review

No abstract provided.

Challenges Under Ocsla And The Future Of Offshore Drilling Under The Obama Administration, Jennifer Larson

SMU Science and Technology Law Review

No abstract provided.

Antidepressant Stimulation Of Cdp-Diacylglycerol Synthesis Does Not Require Monoamine Reuptake Inhibition, Ashiwel S. Undieh, Marwa A. Aboukhatwa

Publications and Research

Background: Recent studies demonstrate that diverse antidepressant agents increase the cellular production of the nucleolipid CDP-diacylglycerol and its synthetic derivative, phosphatidylinositol, in depression-relevant brain regions. Pharmacological blockade of downstream phosphatidylinositide signaling disrupted the behavioral antidepressant effects in rats. However, the nucleolipid responses were resistant to inhibition by serotonin receptor antagonists, even though antidepressant-facilitated inositol phosphate accumulation was blocked. Could the neurochemical effects be additional to the known effects of the drugs on monoamine transmitter transporters? To examine this question, we tested selected agents in serotonin-depleted brain tissues, in PC12 cells devoid of serotonin transporters, and on the enzymatic activity of …

Integrating Marital Property Into A Spouse’S Elective Share, Raymond C. O'Brien

Scholarly Articles

First, this Article begins with history, as this forms the basis of electiveshare law. It is necessary to begin with the historical basis of a spouse's right to support, and then proceed to examine how and why a spouse obtained a share of the property acquired during marriage. Second, because a spouse's rights at death were often very different from those that a spouse would obtain at divorce, it is necessary to explain the various judicial and statutory models adopted by the states to provide a modicum of protection to a surviving spouse at death. There are many models and …

Introducing Stem Majors To The Teaching Profession Through Authentic Experiences As Tutors, Grinell Smith

Faculty Publications

Recruiting people with rich backgrounds in science, technology, engineering, and mathematics (STEM) into the teaching profession has historically been difficult and remains so today. In this bounded instrumental case study, undergraduate STEM majors were trained and placed as science and math tutors in grades 9-12, with an overarching goal of encouraging them to consider teaching as a possible career path. Several themes emerged from the tutors’ experiences as significant in their willingness to consider a career in teaching, including altruism from helping others, satisfaction from tutees’ improved academic performance, and a more nuanced understanding of the complex nature of teaching.

Salinity Tolerance And Osmotic Response Of The Estuarine Hermit Crab Pagurus Maclaughlinae In The Indian River Lagoon, Florida, Sarah E. Rhodes-Ondi, Richard L. Turner

Ocean Engineering and Marine Sciences Faculty Publications

Pagurus maclaughlinae is the most common hermit in the Indian River Lagoon System. Wide variations in lagoonal salinity make it likely that P. maclaughlinae is euryhaline and that other hermit species in the area are more stenohaline, at least in some stages of their life histories. In a study of salinity tolerance, crabs were held unfed at salinities of 5–50 (25 control) for up to 30 d. Based on survivorship curves, P. maclaughlinae tolerated acute exposure to salinities of 10–45 for up to 18 d, and survivorship up to 30 d at 20–45 equaled or exceeded survivorship of the control. …

Hippocampal And Anterior Cingulate Cortex Volumes In Amnestic And Non-Amnestic Mild Cognitive Impairment, Elizabeth R. Tuminello

Master's Theses

Mild Cognitive Impairment (MCI) is a syndrome thought to fall between cognitively normal aging and dementia. Although much research has investigated the structural neuroimaging correlates of amnestic MCI, little research has been done on the imaging correlates of non-amnestic MCI. Even less research has examined the anterior cingulate cortex (ACC), a region important in executive functions (EFs), in these patients. This study attempted to address this gap by examining hippocampal and ACC volumes among amnestic and non-amnestic MCI patients and cognitively normal controls. Those with amnestic MCI were expected to have smaller hippocampal volumes than controls and those with non-amnestic …

The Distribution Of Gnrh And Erbeta In Pre- And Post-Pubertal Male Rats, Maysaa Nageeb

Master's Theses

The hypothalamic-pituitary- gonadal axis is intrinsic in regulating reproductive function. Each part orchestrates the synthesis and release of a key hormone. At the level of the hypothalamus, Gonadotropin- releasing hormone (GnRH) is released and it is the

most upstream regulator of sexual maturation. GnRH neurons express a type of estrogen receptor, ERbeta, which suggests that estradiol (E2) plays a direct role in modulating GnRH function (Herbison and Pape 2001). The objective of this study was to determine if gonadal steroid hormones regulate the expression of ERbeta and GnRH mRNA in the male brain during pubertal development. In-situ hybridization was used …

Dna Replication And Genome Maintenance Of Human Papillomavirus Type 16 In Mammalian Cells, Daraporn Pittayakhajonwut

School of Biological Sciences: Dissertations, Theses, and Student Research

Human papillomavirus (HPV) is a major causative agent of cervical cencer and a number of human cancers. The high‐risk types can be detected in more than 90% of cervical cancer, of which HPV16 is the most common found. The virus establishes a latent infection in basal epithelial cells in which the viral genomes can be stably maintained as extrachromosomal DNA for decades before the development of cancer. Several attempts have been made in bovine papillomavirus (BPV) to understand how the virus can maintain its genome at constant copy numbers in dividing cells. The viral protein E2 has been proposed to …

B Lymphocyte Development In Galt, Venkata Arunachalam Yeramilli

Dissertations

In rabbits, the primary antibody repertoire is generated in the gut-associated lymphoid tissues (GALT), where bone marrow (BM)-derived B cells undergo a proliferative expansion and somatically diversify the immunoglobulin genes. Unlike in other species, B lymphopoiesis in rabbit arrests a few months after birth, and it is unclear how the peripheral B cell compartment is maintained when there is no influx of newly-made B cells from the BM.

For my dissertation, I investigated how B cells develop in the GALT of rabbits, and how they are maintained in adults after the arrest of lymphopoiesis. To identify cellular signals that promote …

Ethanol Impairs Mechanisms Of Macrophage Phagocytosis And Cytokine Production, John Karavitis

Dissertations

Extensive evidence indicates that alcohol (ethanol) consumption affects human health by altering normal physiological functions of the immune system. This study investigated the effect of a single in vivo exposure of macrophages to clinically relevant levels of ethanol (1.2 and 2.2 g/kg). Following 3 hour exposure, both doses of ethanol decreased ex vivo TNFα production by splenic and alveolar macrophages (AMs). Interestingly, the higher dose of ethanol resulted in sustained suppression of LPS-induced TNFα production at 3 and 6 hours post ethanol administration, as well as decreased IL-6 and IL-12 production after 6 hours, compared control treated groups. LPS or …

Your Change Is Still Behind: Futurity In Early Modern Literature, Tripthi Pillai

Dissertations

A study of Renaissance literature's engagement with temporality, my project is a critical evaluation of the concept of early modern futurity, of which I propose three categories: "Material futurity"; "Biological futurity"; and "Political futurity." In the moments that I identify in texts composed during the Tudor and early Stuart reigns in England, I demonstrate that the future--as an idea--structures individuals' actions and ruptures social formations. Futurity, which I define as a play of multiple desires that exist simultaneously within our present beings, is a volatile agent of imagination in early modern literature. Futurity collides with the cultural sites of memory …

Characterization Of Tram, A Required Bacteroides Fragilis Conjugal Apparatus Protein That Interacts With The B. Fragilis Putative Coupling Protein Bcta And The B. Fragilis Relaxase Protein Bmph, Mai Thi Thanh Nguyen

Dissertations

Bacteroides spp. organisms, the most prominent bacteria of the human colon, are reservoirs of antibiotic resistance genes which promiscuously transfer within and from the genus via conjugation. The B. fragilis clinical isolate LV23 harbors a 37kb conjugative transposon, BTF-37. Two important conjugal apparatus proteins encoded by BTF-37 are BctA, a predicted coupling protein (CP) that powers DNA translocation, and ORF7 (TraM). In this study, we demonstrated that TraM exhibits characteristics of a required conjugal apparatus protein including 1) upregulated expression under conjugation conditions; 2) localization to the bacterial inner membrane; 3) interaction with the putative coupling protein BctA and the …

Morphogenesis Of The Bacillus Anthracis Spore, Michael Mallozzi

Dissertations

With the ability to shut down metabolism, survive exposure to high degrees of heat, ultra-violet and ionizing radiation, digestive enzymes, and antimicrobials, the bacterial spore is one of the most-resistant forms of life on earth. Although most spores are harmless, several species' spores cause serious diseases such as tetanus, anthrax, gas gangrene, botulism, and diarrhea. Spore-forming bacteria differentiate into spores in response to stresses (especially starvation) in a multi-stage developmental pathway involving the coordinated expression of hundreds of genes and the formation of specialized protective organelles which surround and protect the spore during dormancy. The Bacillus anthracis spore (the causative …

Involvement Of The Nmda Receptor In Moderate Ethanol Preconditioning-Dependent Neuroprotection From Amyloid-Beta In Vitro, Robert Matthew Mitchell

Dissertations

Alzheimer's disease (AD) is a progressive, mentally crippling, and eventually fatal form of dementia with growing prevalence in aging populations. In 2009 it was estimated that 5.3 million Americans have AD, with 5.1 million older than age 65 and 200,000 under 65. That accounts for 1 in 8 adults over 65. Furthermore, AD costs Americans 148 billion dollars in direct expenses, and is the leading cause of dementia and the 6th leading cause of death. Since improved medical care in general is increasing average life span and age is the primary risk factor for AD, there is need for basic …

Interpretive Teleology Without Eschatology: A Study Of The Hermeneutic Strategies Of Nietzsche's Genealogical Project, Darin Sean Mcginnis

Dissertations

In contemporary philosophy, there exists a vigorous debate between those thinkers who advocate the need for a truth that exists outside of historical forces and those who hold that all understanding must be historical in its basis. My research is focused on deciphering this problem by providing a description of how understanding and interpretation occur. More specifically, I have proposed a project that explores how the philosophical writings of Friedrich Nietzsche can give some insight into this problem of understanding truth. I argue that Nietzsche develops a method of interpretation - a hermeneutics - that focuses on the manner in …

Immigration As A "Theologizing Experience": Spiritual Well-Being As A Moderating Factor In Migratory Grief And Acculturation, Irma A. Sharp

Dissertations

The purpose of this study was to investigate the relationship of spiritual well-being to migratory grief and acculturation. The study employed a cross-sectional design and a convenience sampling method. Data were collected from 75 Mexican immigrant adult education students in the city of Chicago. Instruments used to collect data were a demographic form, Spiritual Well-Being Scale, Migratory Grief and Loss Questionnaire, and the Stephenson Multigroup Acculturation Scale. All instruments were translated into Spanish, the native language of the participants. Multiple regression analysis was used to test the moderator effects of existential well-being and religious well-being, the two sub-scales of the …

The Complexities Of Attachment, Peer Rejection And Gender Within A Middle School Dynamic - A Glimpse At Early Adolescents And Their Significant Relationships, Barbara Ann Mestling

Dissertations

This was a mixed method two phase explanatory study of attachment, peer rejection and gender in a non-clinical middle school population. The purpose of the study was to illustrate the relationship between these variables with the assumption that there would be differences in attachment style between rejected and non-rejected students and further differences by gender. The Behavioral Systems Questionnaire (BSQ) and the Children's Self-Experience Questionnaire-Self-Report (CSEQ-SR) were utilized to determine interview subjects. A Parent Demographic Form provided background, life experience and social functioning information. There were challenges in the use of both instruments for this study's purposes. Although there were …

The Relationship Among Adult Attachment Styles, Shame, Dissociation, And Abuse Characteristics In Women Survivors Of Intrafamilial Child Sexual Abuse Perpetrated By A Parental-Figure, Grace H. Tomas-Tolentino

Dissertations

The consequences of intrafamilial child sexual abuse (CSA) often evolve beyond the termination of abuse and pervade into adulthood, posing complex and enduring consequences for survivors (Courtois, 1999; Briere, 1992; Herman, 1981, 1991; van der Kolk & Kadish, 1987; Chu & Dill, 1990). The concepts of attachment, shame, and dissociation- in the context of childhood sexual abuse-are significant areas of study when working with adult survivors. The present study included 49 women with a history of childhood sexual abuse perpetrated by a parental-figure. The purpose of the study was to identify the relationships among adult attachment styles, shame, dissociation, and …

Roman Law And The Legal World Of The Romans, James G. Keenan

Classical Studies: Faculty Publications and Other Works

A review of Andrew. M Riggsby's book, Roman Law and the Legal World of the Romans.

The Impact Of Person-Centered Communications On Political Candidate Evaluation: An Experimental Investigation, Randall A. Renstrom

Dissertations

"Person-centeredness" refers to how empathetic and warm a person's communication style is. Although the role of person-centeredness has been documented in various areas concerning interpersonal relations, person-centeredness has not been explored in the political realm. This project investigated how person-centered communications can influence impressions and evaluations of political candidates. In the first study, person-centered (PC) messages were shown to impact candidate trait ratings. Candidates using low PC messages were associated with more instrumental traits but fewer socio-emotional traits, while high PC candidates were assumed to have more socio-emotional traits but fewer instrumental traits. Similar results were found when participants rated …

I Am Black And Beautiful: An Examiniaton Of The Black Coptic Church As The Manifestation Of Liberation Theology, Leonard Mckinnis

Dissertations

What does the Black Coptic Church have to offer the study of Black theology? This is the essential question this dissertation seeks to examine through a descriptive and constructive analysis. I submit the doctrinal formulation of black liberation theology is locatable within the Black Coptic Church, which was founded, and still boasts its largest membership in Chicago. This is the tradition that has informed my own faith background and has played a tremendous role in my earlier theological convictions. In fact, I was attracted to this tradition as a young child because it seemed to offer its adherents a sense …

Deconstructing Ihf-Mediated Inhibition Of The Complex Acs Promoter, David S. Thach

Dissertations

acs encodes a high affinity enzyme that permits survival during carbon starvation. As befits a survival gene, its transcription is subject to complex regulation. Previously, the Wolfe lab reported that CRP activates acs transcription by binding tandem DNA sites located upstream of the major acsP2 promoter and that the nucleoid protein IHF binds three specific sites located just upstream. The most proximal site (IHF III) exhibits reduced transcription compared to the full-length promoter or to a construct lacking all three IHF sites. The goal of my research was to understand how IHF III inhibits CRP-dependent acs transcription. First, I helped …

Altgeld Gardens: Evolution Of Culture And Educatiion In An Isolated African American Community, Beverly Anne Lesueur

Dissertations

Altgeld Gardens, a Chicago public housing project, was selected for this study because its unique attributes were designed as a model, self-contained complex located in a geographically and socially, isolated area of Chicago built to house African American war armament workers and their families beginning in the mid-1940’s. Oral testimonies from teachers and community members chronicle significant changes to the local culture and the primary school’s efforts to provide effective instructional services within the cultural context of this community. The most effective and frequently used modification of instruction was the incorporation of culturally relevant pedagogy. The goal was to effectively …