Digital Commons Network^™

A Practical Approach To Assess Depression Risk And To Guide Risk Reduction Strategies In Later Life, Osvaldo Almeida, Helman Alfonso, Jane Pirkis, Ngaire Kerse, Moira Sim, Leon Flicker, John Snowdon, Brian Draper, Gerard Byrne, Robert Goldney, Nicola Lautenschlager, Nigel Stocks, Marcia Scazufca, Martijn Huisman, Ricardo Araya, John Pfaff

Research outputs pre 2011

Background: Many factors have been associated with the onset and maintenance of depressive symptoms in later life, although this knowledge is yet to be translated into significant health gains for the population. This study gathered information about common modifiable and non-modifiable risk factors for depression with the aim of developing a practical probabilistic model of depression that can be used to guide risk reduction strategies. Methods: A cross-sectional study was undertaken of 20,677 community-dwelling Australians aged 60 years or over in contact with their general practitioner during the preceding 12 months. Prevalent depression (minor or major) according to the Patient …

The Pregnancy Hormones Human Chorionic Gonadotropin And Progesterone Induce Human Embryonic Stem Cell Proliferation And Differentiation Into Neuroectodermal Rosettes, Miguel Gallego, Prashob Porayette, Maria Kaltcheva, Richard Bowan, Sivan Vadakkadath Meethal, Craig Atwood

Research outputs pre 2011

Introduction: The physiological signals that direct the division and differentiation of the zygote to form a blastocyst, and subsequent embryonic stem cell division and differentiation during early embryogenesis, are unknown. Although a number of growth factors, including the pregnancy-associated hormone human chorionic gonadotropin (hCG) are secreted by trophoblasts that lie adjacent to the embryoblast in the blastocyst, it is not known whether these growth factors directly signal human embryonic stem cells (hESCs). Methods: Here we used hESCs as a model of inner cell mass differentiation to examine the hormonal requirements for the formation of embryoid bodies (EB’s; akin to blastulation) …

Predicting Memory Decline As A Risk Factor For Alzheimer's Disease In Older Post-Menopausal Women: Quod Erat Demonstrandum?, Mark Rodrigues, Jonathan Foster, Giuseppe Verdile, Karen Joesbury, Richard Prince, Amanda Devine, Pankaj Mehta, John Beilby, Ralph Martins

Research outputs pre 2011

Alzheimer's disease (AD) is the major form of age-related dementia worldwide, accounting for more than two-thirds of all dementia cases. The disease is characterized by a progressive loss of cognitive and intellectual functioning (Gilman, 1997). A number of risk factors for AD have been identified. The prevalence of AD increases with age, diabetes, depression, family history of Parkinson's disease and following head injury or exposure to solvents (Jorm et al., 1991; van Duijn et al., 1991; Ott et al., 1995; Yoshitake et al., 1995; Devanand et al., 1996). Published research further suggests that low education levels are associated with increased …

Extensive Proteomic Screening Identifies The Obesity-Related Nyggf4 Protein As A Novel Lrp1-Interactor, Showing Reduced Expression In Early Alzheimer's Disease, Yuji Kajiwara, Sonia Franciosi, Nagahide Takahashi, Lisa Krug, James Schmeidler, Kevin Taddei, Vahram Haroutunian, Ulrik Fried, Michelle Ehrlich, Ralph Martins, Samuel Evans Gandy

Research outputs pre 2011

Background The low-density lipoprotein receptor related protein 1 (LRP1) has been implicated in Alzheimer's disease (AD) but its signalling has not been fully evaluated. There is good evidence that the cytoplasmic domain of LRP1 is involved in protein-protein interactions, important in the cell biology of LRP1. Results We carried out three yeast two-hybrid screens to identify proteins that interact with the cytoplasmic domain of LRP1. The screens included both conventional screens as well as a novel, split-ubiquitin-based screen in which an LRP1 construct was expressed and screened as a transmembrane protein. The split-ubiquitin screen was validated in a screen using …

Host Recognition And Acceptance Behaviour In Cotesia Sesamiae And C. Flavipes (Hymenoptera: Braconidae), Parasitoids Of Gramineous Stemborers In Africa, Meshack Obonyo, Fritz Schulthess, Bruno Le Ru, Johnnie Van Den Berg, Paul André Calatayud

All Peer-Reviewed Publications

The host recognition and acceptance behaviour of two braconid larval parasitoids (Cotesia sesamiae and C. flavipes) were studied using natural stemborer hosts (i.e., the noctuid Busseola fusca for C. sesamiae, and the crambid Chilo partellus for C. flavipes) and a non-host (the pyralid Eldana saccharina). A single larva was introduced into an arena together with a female parasitoid and the behaviour of the wasp recorded until it either stung the larva or for a maximum of 5 min if it did not sting the larva. There was a clear hierarchy of behavioural steps, which was similar for both parasitoid species. …

Synesthetic Landscapes In Harold Pinter’S Theatre: A Symbolist Legacy, Graça Corrêa

Dissertations, Theses, and Capstone Projects

In the light of recent interdisciplinary critical approaches to landscape and space, and adopting phenomenological methods of sensory analysis, this dissertation explores interconnected or synesthetic sensory "scapes" in contemporary British playwright Harold Pinter's theatre. By studying its dramatic landscapes and probing into their multi-sensory manifestations in line with Symbolist theory and aesthetics, I argue that Pinter's theatre articulates an ecocritical stance and a micropolitical critique.

Chapter One explains the dissertation's theoretical framework (landscape theory, Symbolist theory, ecocriticism, phenomenology, and sensory analysis), while arguing for an ecophilosophical reading of Pinter's landscapes that engages not only with spatial patterns but …

"If You See Something, Say Something": The Power Of The 'War On Terrorism' To Name What We See, Polly Sylvia

Dissertations, Theses, and Capstone Projects

This dissertation seeks to understand the cultural politics of the "war on terrorism" through a case study of the "If You See Something, Say Something" campaign within the New York City Metropolitan Transit Authority Subway System. Drawing upon literature that focuses on an understanding of the affective transmission of culture, this research seeks to understand this particular campaign as a technique of social control. Through a content analysis of the advertisements of this campaign and a performative methodology that analyzes the performance of security within the subway system, an understanding of the connections this local campaign (as a security campaign) …

Madness, Sexuality, And Gender In Early Twentieth Century Music Theater Works: Four Interpretive Essays, Megan B. Jenkins

Dissertations, Theses, and Capstone Projects

Diagnoses of madness are inextricably entwined with social and cultural beliefs about gender and sexual behavior. The portrayal of characters in music theater as mad relies on contemporaneous understanding of mental illness, as often resulting from, or expressed in transgression of normative gender roles or heteronormativity, and this may apply either to male or female characters. Such transgressions are explored--with regard to recent reconceptualizations of madness within Disability Studies--in four works: Arnold Schoenberg's monodrama Erwartung (1924); Richard Strauss's opera Salome (1905); Kurt Weill's ballet chanté, Anna-Anna (1933), also known as The Seven Deadly Sins; and Igor Stravinsky's neo-classical …

Effect Of Pineapple (Ananas Comosus L. Merrill) And Papaya (Carica Papaya L.) Fruit Extracts On Sericin Removal From Silk Moths Cocoons In Kenya, Pauline Wairimu Wainaina

All Master’s Theses

Sericulture (Silk farming) is the rearing of silk moths for the production of raw silk. Silk farming originated from China where it was secretly practiced for many years before spreading to other parts of the world. Silk moths are grouped into mulberry silk moths (domesticated) and wild silk moths. Silk moth cocoon has a double filament made of insoluble protein known as fibroin which is embedded in a water soluble protein called sericin. Prior to processing cocoons, are boiled in strong alkali so as to dissolve the sericin a process called degumming. Proteinases enzymes such as papain from papaya (Carica …

Morphormetrics And Dna Barcoding For The Identification Of Wild Silk Moths From Selected Sites In Kenya, Peter Kuria Kamau

All Master’s Theses

Wild silk moth farming is a unique eco-friendly agro-practice with the potential of raising people's standards of living. However, the species identity is poorly understood in East Africa. This undermines efforts in conservation practices. Accurate identification is important in understanding the biology and ecology of the different species. Cocoons of wild silk moths were collected from Eastern and Rift valley provinces in Kenya. The samples included Gonometa species, Epiphora bauhiniae, and Argema besanti. Morphometric studies of the Kenyan wild silk moth species were performed based on three morphometric characters, which included forewing length, forewing width and body length measurements. Voucher …

A Few Inconvenient Truths About Michael Crichton's State Of Fear: Lawyers, Causes And Science, Lea B. Vaughn

Articles

Although Crichton has lost the battle regarding global warming, his characterization of lawyers and law practice remains unchallenged. This article challenges his damning portrait of lawyers as know-nothing, self-aggrandizing manipulators of various social and environmental causes. A more nuanced examination of "cause lawyering" reveals that lawyers are not part of a vast conspiracy to grab power through the causes for which many work; in fact, the rules of professional responsibility as well as the structure of "cause lawyering" limit their power and influence. Regardless, lawyers are nonetheless vital, and generally principled, participants in the debates and causes that inform environmental …

Carbon Capture And Storage In South Dakota: The Need For A Clear Designation Of Pore Space Ownership, Blayne N. Grave

South Dakota Law Review

No abstract provided.

Oxidative Stress Of Burkholderia Cenocepacia Induces Insertion Sequence-Mediated Genomic Rearrangements That Interfere With Macrorestriction-Based Genotyping, Pavel Drevinek, Adam Baldwin, Laurens Lindenburg, Lovleen Tina Joshi, Angela Marchbank, Sarka Vosahlikova, Christopher G. Dowson, Eshwar Mahenthiralingam

Peninsula Dental School

ABSTRACT Burkholderia cenocepacia can cause serious infections and epidemics in patients with cystic fibrosis (CF). A CF population in the Czech Republic experienced an epidemic outbreak caused by a B. cenocepacia ST-32 strain. The clonality of the isolates was evident by multilocus sequence typing; however, fingerprinting profiles obtained by pulsed-field gel electrophoresis (PFGE) showed substantial band variability. We investigated whether the PFGE pattern diversity resulted from genomic rearrangements mediated by insertion sequences (IS); in addition, we determined whether stressful growth conditions altered the transposition activity of these IS. DNA probes for IS commonly found in B. cenocepacia were designed using …

Phylogeographic Patterns And Intervarietal Relationships Within Lupinus Lepidus: Morphological Differences, Genetic Similarities, Kevin Allen Weitemier

Dissertations and Theses

Lupinus lepidus (Fabaceae) contains many morphologically divergent varieties and was restricted in its range during the last period of glaciation. A combination of phylogenetic (with the trnDT and LEGCYC1A loci) and population genetics approaches (with microsatellites and LEGCYC1A are used here to characterize intervarietal relationships and examine hypotheses of recolonization of areas in the Pacific Northwest affected by glaciation. Sequenced loci are not found to form a clade exclusive to L. lepidus, nor are any of the varieties found to form clades. Population genetics analyses reveal only negligible genetic structure within L. lepidus, with the majority of variation …

Understanding The Development Of Self-Determination In Youth With Disabilities In Foster Care, Jennifer L. Powers

Dissertations and Theses

Youth in foster care who experience disabilities face many challenges as they transition out of foster care and into adulthood. In order to assist these youth, it is crucial to understand factors that may impact their self-determination, which research links to positive transition outcomes for youth with disabilities (Wehmeyer, Palmer, Agran, Mithaug, & Martin, 2000). While much of the existing research on the correlates and outcomes of self-determination focuses on young people with disabilities overall, and little is known about whether factors such as abuse, family stressors and extended length of time in care, and frequent placement changes influence self-determination. …

Communication And Essentialism: Grounding The Shared Reality Of A Social Category, Yoshihisa Kashima, Emiko S. Kashima, Paul Bain, Anthony Lyons, R Scott Tindale, Gary Robins, Cedric Vears, Jennifer Whelan

Psychology: Faculty Publications and Other Works

Essentialism is an ontological belief that there exists an underlying essence to a category. This article advances and tests in three studies the hypothesis that communication about a social category, and expected or actual mutual validation, promotes essentialism about a social category. In Study 1, people who wrote communications about a social category to their ingroup audiences essentialized it more strongly than those who simply memorized about it. In Study 2, communicators whose messages about a novel social category were more elaborately discussed with a confederate showed a stronger tendency to essentialize it. In Study 3, communicators who elaborately talked …

Teaching Species, Mark W. Ellis, Paul G. Wolf

Biology Faculty Publications

A clear understanding of the term "species" is fundamental to the subject of evolution. However, introductory textbooks often fail to address this topic until one of the later chapters, after having used the term species in all preceding chapters. Furthermore, definitions of terms critical to a clear understanding of this subject are often vague or absent in chapters on species concepts. We feel the popular notion of a "species problem" has been unnecessarily inflated by this less-than-effective educational approach. Clearly addressing this essential subject at the beginning of a course on evolution will prepare students to learn the details and …

Complete Plastome Sequences Of Equisetum Arvense And Isoetes Flaccida: Implications For Phylogeny And Plastid Genome Evolution Of Early Land Plant Lineages, K. G. Karol, K. Arumuganthan, J. L. Boore, A. M. Duffy, K. D. E. Everett, J. D. Hall, S. K. Hansen, J. V. Mandoli, D. F. Mandoli, B. D. Mishler, R. G. Olmstead, K. S. Renzaglia, Paul G. Wolf

Biology Faculty Publications

Background Despite considerable progress in our understanding of land plant phylogeny, several nodes in the green tree of life remain poorly resolved. Furthermore, the bulk of currently available data come from only a subset of major land plant clades. Here we examine early land plant evolution using complete plastome sequences including two previously unexamined and phylogenetically critical lineages. To better understand the evolution of land plants and their plastomes, we examined aligned nucleotide sequences, indels, gene and nucleotide composition, inversions, and gene order at the boundaries of the inverted repeats. Results We present the plastome sequences of Equisetum arvense, a …

The Evolution Of Chloroplast Genome Structure In Ferns, Paul G. Wolf, J. M. Roper, A. M. Duffy

Biology Faculty Publications

The plastid genome (plastome) is a rich source of phylogenetic and other comparative data in plants. Most land plants possess a plastome of similar structure. However, in a major group of plants, the ferns, a unique plastome structure has evolved. The gene order in ferns has been explained by a series of genomic inversions relative to the plastome organization of seed plants. Here, we examine for the first time the structure of the plastome across fern phylogeny. We used a PCR-based strategy to map and partially sequence plastomes. We found that a pair of partially overlapping inversions in the region …

Chloroplast Genome Sequence Of The Moss Torula Ruralis: Gene Content, Polymorphism, And Structural Arrangement Relative To Other Green Plant Chloroplast Genomes, M. J. Oliver, A. G. Murdock, B. D. Mishler, J. V. Kuehl, J. L. Boore, D. F. Mandoli, K. D. E. Everett, Paul G. Wolf, A. M. Duffy, K. G. Karol

Biology Faculty Publications

Background Tortula ruralis, a widely distributed species in the moss family Pottiaceae, is increasingly used as a model organism for the study of desiccation tolerance and mechanisms of cellular repair. In this paper, we present the chloroplast genome sequence of T. ruralis, only the second published chloroplast genome for a moss, and the first for a vegetatively desiccation-tolerant plant. Results The Tortula chloroplast genome is ~123,500 bp, and differs in a number of ways from that of Physcomitrella patens, the first published moss chloroplast genome. For example, Tortula lacks the ~71 kb inversion found in the large single copy region …

Unfurling Fern Biology In The Genomics Age, M. S. Barker, Paul G. Wolf

Biology Faculty Publications

Twenty-first century technology is addressing many of the questions posed by 20th-century biology. Although the new approaches, especially those involving genomic data and bioinformatic tools, were first applied to model organisms, they are now stretching across the tree of life. Here, we review some recent revelations in the ferns. We first examine how DNA sequence data have contributed to our understanding of fern phylogeny. We then address evolution of the fern plastid genome, including reports of high levels of RNA editing. Recent studies are also shedding light on the evolution of fern nuclear genomes. Initial analyses of genomic data suggest …

Credible Utility In Patent Law, Martin J. Adelman

GW Law Faculty Publications & Other Works

This paper discusses the law relating to the patentability of products and methods of their use. Specifically, the paper examines the circumstance where an inventor has brought forth a product which has no known credible utility or industrial applicability, but adds some guesses to her patent application in the hopes that if she guesses correctly, she will obtain a valuable patent. If one of the guesses proves correct, should this be treated in essence as a constructive reduction to practice sufficient to justify the grant of a valid patent?

This paper suggests that the proper answer should be no because …

Regulatory Blowout: How Regulatory Failures Made The Bp Disaster Possible, And How The System Can Be Fixed To Avoid A Recurrence, Robert L. Glicksman

GW Law Faculty Publications & Other Works

The BP oil spill in the Gulf of Mexico is destined to take its place as one of the greatest environmental disasters in the history of the United States, or for that matter, of the entire planet. Like so many other disasters on that list, it was entirely preventable.

BP must shoulder its share of the blame, of course. Similarly, the Minerals Management Service (MMS) – since reorganized and rebranded – has come under much deserved criticism for its failure to rein in BP’s avaricious approach to drilling even where it was unable to respond to a worst-case scenario in …

Climate Change Adaptation: A Collective Action Perspective On Federalism Considerations, Robert L. Glicksman, Richard E. Levy

GW Law Faculty Publications & Other Works

The buildup of greenhouse gases in the atmosphere and the likely growth in future emissions due to increased energy consumption in developing nations have convinced many scientists and policymakers of the need to develop policies that will allow adaptation to minimize the adverse effects of climate change. Climate change adaptation is designed to increase the resilience of natural and human ecosystems to the threats posed by a changing environment. Although an extensive literature concerning the federalism implications of climate change mitigation policy has developed, less has been written about the federalism issues arising from climate change adaptation policy. This article …

Terrorism And The Law: Cases And Materials, Gregory E. Maggs

GW Law Faculty Publications & Other Works

Terrorism and the Law: Cases and Materials (2d ed. 2010) is a textbook written by Professor Gregory E. Maggs (of the George Washington University Law School) and published by West (ISBN-13: 9780314908582).

The textbook considers legal aspects of a broad range of methods that governments have for fighting terrorism, including criminal penalties, economic sanctions, immigration restrictions, military force, and civil liability. It addresses not just the steps taken in reaction to the 9/11 attacks, but also many other counterterrorism measures by the United States and other nations in recent years. To offer a global and comparative perspective, the materials include …

Embryo Fundamentalism, Naomi R. Cahn, June Caborne

GW Law Faculty Publications & Other Works

The battle for the future of assisted reproduction technologies (ART) has been joined. The tacit compromise underlying assisted reproduction - no laws are passed that even tangentially sanction embryo destruction and no laws are passed that intrude on the profitability of fertility treatments - may be coming to an end. As use of ART has increased, so have calls for supervision and oversight. In the wake of "Octomom" Nadya Suleman's use of in vitro fertilization (IVF) to give birth to octuplets, the calls to regulate assisted reproduction have become even more pressing. At the same time, religious communities ambivalent about …

In Vitro Mammalian Cytotoxicological Study Of Pamam Dendrimers -Towards Quantitative Structure Activity Relationships, Sourav Prasanna Mukherjee, Maria Davoren, Hugh Byrne

Articles

Dendritic polymer nanoparticles such as polyamidoamine dendrimers (PAMAM) show exciting potential for biomedical applications. While the potential commercial applications of such dendrimers have received considerable attention, little is known about their possible adverse effects on both humans and the environment. In this study, the in vitro cytotoxicocity of full generation PAMAM dendrimers to two mammalian cell lines was investigated. Generations G4, G5 and G6 were chosen. Metabolic, lysosomal and mitochondrial activities were evaluated after 24 h exposure. Long term toxicity was evaluated using the clonogenic assay. Particle size and zeta potential were measured in all media. In culture medium, the …

Resonant Mie Scattering (Rmies) Correction Of Infrared Spectra From Highly Scattering Biological Samples Analyst, Paul Bassan, Achim Kohler, Harald Martens, Joe Lee, Hugh Byrne, Paul Dumas, Ehsan Gazi, Michael Brown, Noel Clarke, Peter Gardner

Articles

Infrared spectra of single biological cells often exhibit the “dispersion artefact” observed as a sharp decrease in intensity on the high wavenumber side of absorption bands, in particular the Amide I band at ~1655 cm-1, causing a downward shift of the true peak position. The presence of this effect makes any biochemical interpretation of the spectra unreliable. Recent theory has shed light on the origins of the ‘dispersion artefact’ which has been attributed to resonant Mie scattering (RMieS). In this paper a preliminary algorithm for correcting RMieS is presented and evaluated using simulated data. Results show that the ‘dispersion artefact’ …

Drug-Eluting Stents For Coronary Artery Disease: A Review, David Martin, Fergal Boyle

Articles

Over the past decade the introduction of drug-eluting stents (DESs) has revolutionised the treatment of coronary artery disease. However, in recent years concern has arisen over the long-term safety and efficacy of DESs due to the occurrence of late adverse clinical events such as stent thrombosis. With this concern in mind, research and development is currently centred on increasing the long-term safety and efficacy of DESs. The aim of this paper is to provide a thorough review of currently approved and promising investigational DESs. With dozens of companies involved in the development of new and innovative anti-restenotic agents, polymeric coatings …

The Effects Of Dietary Ascorbic Acid Supplementation On Collagen And Amino Acid Concentrations In Japanese Quails Exposed To Heat Stress, Kami̇l Seyrek, Hakan Erbaş, Mukadder Serter, Çi̇ğdem Yeni̇sey, Funda Kiral, Hüsnü Erbay Bardakçioğlu

Turkish Journal of Veterinary & Animal Sciences

A considerable body of data exists regarding the role of vitamin C in mammalian physiology; however, there are no data about the effects of dietary ascorbic acid supplementation on collagen concentrations and amino acid levels in animals exposed to heat stress. The present study investigated the effects of supplementary ascorbic acid intake on collagen concentrations in the brain and heart tissue in Japanese quails. In addition, glycine, glutamine, histidine, asparagine, and serine contents in the livers of the same animals were measured. Japanese quails were allocated into 4 groups, each of which was exposed to heat stress (34.8 ± 1.25 …