Digital Commons Network^™

Development Of Novel Ahr Antagonists, Hyosung Lee

University of Kentucky Doctoral Dissertations

Aryl hydrocarbon receptor (AHR) is a sensor protein, activated by aromatic chemical species for transcriptionally regulating xenobiotic metabolizing enzymes. AHR is also known to be involved in a variety of pathogenesis such as cancer, diabetes mellitus, cirrhosis, asthma, etc. The AHR signaling induced by xenobiotics has been intensively studied whereas its physiological role in the absence of xenobiotics is poorly understood. Despite a number of ligands of AHR have been reported thus far, further applications are still hampered by the lack of specificity and/or the partially agonistic activity. Thus, a pure AHR antagonist is needed for deciphering the AHR cryptic …

Evaluation And Enhancement Of Seed Lot Quality In Eastern Gamagrass [Tripsacum Dactyloides (L.) L.], Cynthia Hensley Finneseth

University of Kentucky Doctoral Dissertations

Eastern gamagrass [Tripsacum dactyloides (L.) L.] is a warm-season, perennial grass which is native to large areas across North America. Cultivars, selections and ecotypes suitable for erosion control, wildlife planting, ornamental, forage and biofuel applications are commercially available. Plantings are commonly established from seed; however, seed quality and dormancy are barriers to establishment. The objective here was to investigate parameters that contribute to inconsistent performance of this seed kind to subsequently improve seed lot quality.

Forty-two seed lots were used, with seed fill and germination potential ranging from 78- 100% and 11-91%, respectively. Actual germination and dormant seed ranged …

Biochemical Characterization Of Human Mismatch Recognition Proteins Mutsα And Mutsβ, Lei Tian

University of Kentucky Doctoral Dissertations

The integrity of an organism's genome depends on the fidelity of DNA replication and the efficiency of DNA repair. The DNA mismatch repair (MMR) system, which is highly conserved from prokaryotes to eukaryotes, plays an important role in maintaining genome stability by correcting base-base mismatches and insertion/deletion (ID) mispairs generated during DNA replication and other DNA transactions. Mismatch recognition is a critical step in MMR. Two mismatch recognition proteins, MutSα (MSH2-MSH6 heterodimer) and MutSβ (MSH2-MSH3 heterodimer), have been identified in eukaryotic cells. MutSα and MutSβ have partially overlapping functions, with MutSα recognizing primarily base-base mismatches and 1-2 nt ID mispairs …

The Role Of Racial Information In Infant Face Processing, Angela Nicole Hayden

University of Kentucky Doctoral Dissertations

The present research addressed the development of specialization in face processing in infancy by examining the roles of race and emotion. An other-race face among own-race faces draws adults’ attention to a greater degree than an own-race face among other-race faces due to the “other-race” feature in other-race faces. This feature underlies race-based differences in adults’ face processing. The current studies investigated the development of this mechanism as well as the influence that this mechanism has on emotion processing in infancy.

In Experiment 1, Caucasian 3.5- and 9- month-olds exhibited a preference for a pattern containing an Asian face among …

Explorations In Homeoviscous Adaptation And Mass Spectral Analysis Of Membrane Lipids, Michael Douglas Timmons

University of Kentucky Doctoral Dissertations

The focus of this dissertation is centered on the mass spectral analysis of lipids and changes occurring in keeping with the concept of homeoviscous adaptation [1]. Homeoviscous adaptation is the process of modification of membrane lipids in response to environmental stimuli [1]. Dissertation investigations applied this concept to prokaryotic and eukaryotic organisms, and expanded the perception of environmental factors from exogenous organic solvents to intracellular environment.

The field of lipidomics deals with the analysis of phospholipid and fatty acid components of membranes the changes that occur due to environmental stimuli and their biological significance [2-6]. The high sensitivity of mass …

Morphology And Systematics Of Braconid Wasps, Charles Andrew Boring

University of Kentucky Doctoral Dissertations

The following morphological structures of the ovipositor of Homolobus truncator (Say) (Hymenoptera : Braconidae) are described and hypotheses of their functions are proposed: a series of sharp ridges on the distal surface of the notch helps maintain a grip on the inner surface of the host cuticle; the sperone directs eggs away from the inner surface of the ventral valves; a flap-like structure on each ventral valve covers the portal through which eggs pass; the valvillus maintains position of the egg within the ovipositor and acts against the egg to force it out; ctenidia on the inner surface of the …

Nm23-H1 Blocks Cell Motility Independently Of Its Known Enzymatic Activities In A Cohort Of Human Melanoma Cells, Joseph Robert Mccorkle

University of Kentucky Doctoral Dissertations

The metastasis suppressor gene NM23-H1 has been shown to possess three enzymatic activities including nucleoside diphosphate kinase, histidine-dependent protein kinase and 3’-5’ exonuclease activity. While these properties have been demonstrated in vitro using recombinant proteins, the contribution of these activities to suppression of metastatic dissemination is unknown. Site-directed mutagenesis studies were used to identify amino acid residues which are required for proper function of each enzymatic activity associated with H1, providing a platform for studying the importance of each function on an individual basis. To assess the relevance of these activities to melanoma progression, a panel of mutants harboring selective …

Late Pleistocene-Early Holocene Colonization And Regionalization In Northern Perú: Fishtail And Paiján Complexes Of The Lower Jequetepeque Valley, Greg J. Maggard

University of Kentucky Doctoral Dissertations

Until relatively recently, the view of Late Pleistocene hunter-gatherers in the Americas was dominated by the “Clovis-first” paradigm. However, recent discoveries have challenged traditional views and forced reconsiderations of the timing, processes, and scales used in modeling the settlement of the Americas. Chief among these discoveries has been the recognition of a wide range of early cultural diversity throughout the Americas that is inconsistent with previously held notions of cultural homogeneity.

During the Late Pleistocene-Early Holocene, the development of widely varying economic, technological and mobility strategies in distinct environments is suggestive of a range of different adaptations and traditions.

It …

Dissecting The Biosyntheses Of Gilvocarcins And Ravidomycins, Madan Kumar Kharel

University of Kentucky Doctoral Dissertations

Gilvocarcin V (GV) and ravidomycin (RMV) exhibit excellent antitumor activities in the presence of near-UV light at low concentration maintaining a low in vivo cytotoxicity. Although, the exact molecular mechanism for in vivo actions of these antibiotics has yet to be determined, a [2+2] cycloaddition reaction of the vinyl side chain with DNA thymidine residues in addition to the inhibition of topoisomerase II and DNAhistone H3 cross-linking are reported for the GV’s mechanism of action. Such activities have made these molecules interesting candidates for the biosynthetic investigation to generate analogues with improved activity/solubility. Previous biosynthetic studies have suggested that the …

Localized Mechanical Deformation And Dissolution Of 45s5 Bioglass, Ding Li

University of Kentucky Doctoral Dissertations

Bioactive glasses react with the human physiological solution in control of their biofunctionality. The stress state in bioactive glasses determines the chemomechanical reaction and their biofunctionality. Using the microindentation technique, the effect of the indentation deformation on the surface damage and material dissolution of 45S5 bioglass was investigated.

The indentation-induced residual stresses were calculated. Complete anelastic recoveries of the indentation depths and the impression marks were observed for the first time, which was likely driven by the stored strain energy over the anelastic deformation zone. The indentation-induced local surface damages were revealed before and after the immersion tests in phosphate …

Analysis Of The Crmp Gene In Drosophila: Determining The Regulatory Role Of Crmp In Signaling And Behavior, Deanna Hardt Morris

University of Kentucky Doctoral Dissertations

The mammalian genome encodes five collapsin response mediator protein (CRMP) isoforms. Cell culture studies have shown that the CRMPs mediate growth cone dynamics and neuron polarity through associations with a variety of signal transduction components and cytoskeletal elements. CRMP is also a member of a protein family including the presumably ancestral dihydropyrimidinase (DHP) protein that catalyzes the second step in pyrimidine degradation. In Drosophila, CRMP and DHP proteins are produced by alternatively spliced transcripts of the CRMP gene. The alternative protein forms have a 91% sequence identity, but unique expression patterns. CRMP is found exclusively in neuronal tissues and …

Evolution And The End Of A World, David Edward Long

University of Kentucky Doctoral Dissertations

This dissertation examines college student understanding and attitudes toward biological evolution. In ethnographic work, I followed a cohort of 31 students through their required introductory biology class. In interviews, students discuss their life history with the concept - in school, at home, at church, and in their communities. For some Creationist students, confronting evolution in class has meant confronting existential issues regarding both the basis of science and the basis of faith. For other Creationist students, claims of evolution's theoretical strength are eschewed for its direct challenge to their worldview. For most students, science holds minimal interest against other values …

Synthesis And Characterization Of Magnetic Hydrogel Nanocomposites For Cancer Therapy Applications, Samantha Ann Meenach

University of Kentucky Doctoral Dissertations

Currently, cancer is the second leading cause of death in the United States. Conventional cancer treatment includes chemotherapy, radiation, and surgical resection, but unfortunately, all of these methods have significant drawbacks. Hyperthermia, the heating of cancerous tissues to between 41 and 45°C, has been shown to improve the efficacy of cancer therapy when used in conjunction with irradiation and/or chemotherapy. In this work, a novel method for remotely administering heat is presented. This method involves heating of tumor tissue using hydrogel nanocomposites containing magnetic nanoparticles which can be remotely heated upon exposure to an external alternating magnetic field (AMF). The …

Cognitive Therapy For The Treatment Of Depressive Symptoms In Patients With Heart Failure, Rebecca L. Dekker

University of Kentucky Doctoral Dissertations

Depressive symptoms are common in patients with heart failure (HF) and adversely affect mortality, morbidity, and health-related quality of life. Cognitive therapy (CT) has been proposed as a non-pharmacological treatment for depressive symptoms in patients with HF. However, there is currently little evidence to support use of CT in patients with HF.

The purpose of this dissertation was to develop and test a brief, nurse-delivered CT intervention for the treatment of depressive symptoms in patients with HF. Prior to testing the intervention, preliminary work was conducted resulting in four manuscripts: 1) a review of the evidence for CT in treating …

In Vivo Oxidative Stress In Alzheimer Disease Brain And A Mouse Model Thereof: Effects Of Lipid Asymmetry And The Single Methionine Residue Of Amyloid-Β Peptide, Miranda Lu Bader Lange

University of Kentucky Doctoral Dissertations

Studies presented in this dissertation were conducted to gain more insight into the role of phospholipid asymmetry and amyloid-β (Aβ)-induced oxidative stress in brain of subjects with amnestic mild cognitive impairment (aMCI) and Alzheimer disease (AD). AD is a largely sporadic, age-associated neurodegenerative disorder clinically characterized by the vast, progressive loss of memory and cognition commonly in populations over the age of ~65 years, with the exception of those with familial AD, which develop AD symptoms as early as ~30 years-old. Neuropathologically, both AD and FAD can be characterized by synapse and neuronal cell loss in conjunction with accumulation of …

Environmental Effects On Behavior And Physiology In Crayfish, Sonya M. Bierbower

University of Kentucky Doctoral Dissertations

Despite dramatic morphological differences between animals from different taxa, several important features in organization and sensory system processing are similar across animals. Because of this similarity, a number of different organisms including mammals, insects, and decapod crustaceans serve as valuable model systems for understanding general principles of environmental effects. This research examines intrinsic and extrinsic factors by behaviorally and physiologically means to identify the impact of environmental conditions on two distinct crayfish species- Procambarus clarkii (surface) and Orconectes australis packardi (cave).

The research identified behavioral and physiological responses in these two morphological and genetically distinct species. The studies also examined …

Building Sustainable Societies: Exploring Sustainability Policy And Practice In The Age Of High Consumption, Cindy Isenhour

University of Kentucky Doctoral Dissertations

This dissertation is an attempt to examine how humans in wealthy, post-industrial urban contexts understand sustainability and respond to their concerns given their sphere of influence. I focus specifically on sustainable consumption policy and practice in Sweden, where concerns for sustainability and consumer-based responses are strong. This case raises interesting questions about the relative strength of sustainability movements in different cultural and geo-political contexts as well as the specific factors that have motivated the movement toward sustainable living in Sweden.

The data presented here supports the need for multigenic theories of sustainable consumerism. Rather than relying on dominant theories of …

Shame, Guilt, And Knowledge Of Hpv In Women Recently Diagnosed With Hpv-Related Cervical Intraepithelial Neoplasia (Cin), Sarah E. Flynn

University of Kentucky Doctoral Dissertations

The current study investigated the relationships between state shame, guilt, and disease knowledge in women recently diagnosed with cervical intraepithelial neoplasia (CIN) caused by the human papillomavirus (HPV). Recent research has indicated that diagnosis of HPV can elicit negative self-directed affect, including persistent experiences of shame. Studies have also shown that knowledge of HPV is low in the general population, even though it is the most common sexually transmitted infection. It is important to understand how shame affects those with HPV because shame is related to a decline in important immune parameters that may be essential in HPV clearance. A …

Reflections On The Movement Toward A More Child-Centered Adoption, Annette R. Appell

Western New England Law Review

No abstract provided.

Family Law—Maternal And Joint Custody Presumptions For Unmarried Parents: Constitutional And Policy Considerations In Massachusetts And Beyond, Bernardo Cuadra

Western New England Law Review

No abstract provided.

Effect Of Ingestion By Alligator Snapping Turtles (Macrochelys Temminckii) On Seeds Of Riparian Vegetation, Jean Pierre Elbers

Graduate Theses/Dissertations

The alligator snapping turtle (Macrochelys temminckii) is a large freshwater turtle that inhabits many lotic water bodies in the Southeastern United States. The species consumes primarily fish but also consumes large amounts of vegetation including seeds of common persimmon (Diospyros virginiana), tupelos (Nyssa sp.), willow oak (Quercus phellos), and pecan (Carya illinoensis). Captive specimens of M. temminckii were fed samples of the above-mentioned seeds to assess how the species affects ingested seeds in order to evaluate the potential role this species may play as a seed disperser. The proportion of seeds defecated intact varied with species (57−99 %), was lowest …

Emerging Law Addressing Climate Change And Water, Elizabeth Burleson

Elisabeth Haub School of Law Faculty Publications

The World Economic Forum recognizes that while restrictions on energy affect water systems and vise versa, energy and water policy are rarely coordinated. The International Panel on Climate Change predicts that wet places will become wetter and dry places will become dryer. Transboundary water, energy and climate coordination can occur through international consensus building.

Significant Statistics: The Unwitting Policy Making Of Mathematically Ignorant Judges, Michael I. Meyerson, William Meyerson

All Faculty Scholarship

This article will explore several areas in which judges, hampered by their mathematical ignorance, have permitted numerical analysis to subvert the goals of our legal system. In Part II, I will examine the perversion of the presumption of innocence in paternity cases, where courts make the counter-factual assumption that regardless of the evidence, prior to DNA testing, a suspect has a 50/50 chance of being the father. In Part III, I will explore the unnecessary injection of race into trials involving the statistics of DNA matching, even when race is entirely irrelevant to the particular case. Next, in Part IV, …

Prognostic Significance Of Trail Death Receptors In Middle Eastern Colorectal Carcinomas And Their Correlation To Oncogenic Kras Alterations, Prashant Bavi, Sarita E Prabhakaran, Jehad Abubaker, Zeeshan Qadri, Thara George, Nasser Al-Sanea, Alaa Abduljabbar, Luai H Ashari, Samar Alhomoud, Fouad Al-Dayel, Azhar Hussain, Shahabuddin, Khawla S Al-Kuraya

Centre for Regenerative Medicine & Stem Cell Research

Background: Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) is a member of the tumour necrosis factor cytokine family that induces apoptosis upon binding to its death domain containing receptors, TRAIL receptor 1 (DR4) and TRAIL receptor 2 (DR5). Expression of TRAIL receptors is higher in colorectal carcinoma (CRC) as compared to normal colorectal mucosa and targeted therapy with TRAIL leads to preferential killing of tumor cells sparing normal cells.
Methods: We investigated the expression of TRAIL and its receptors in a tissue microarray cohort of 448 Middle Eastern CRC. We also studied the correlation between TRAIL receptors and various clinico-pathological …

Dna And Due Process, Brandon L. Garrett

Fordham Law Review

The U.S. Supreme Court in District Attorney’s Office v. Osborne confronted novel and complex constitutional questions regarding the postconviction protections offered to potentially innocent convicts. Two decades after DNA testing exonerated the first inmate in the United States, the Court heard its first claim by a convict seeking DNA testing that could prove innocence. I argue that, contrary to early accounts, the Court did not reject a constitutional right to postconviction DNA testing. Despite language suggesting the Court would not “constitutionalize the issue” by announcing an unqualified freestanding right, Chief Justice Roberts’s majority opinion proceeded to carefully fashion an important, …

The Internet Is A Semicommons, James Grimmelmann

Fordham Law Review

No abstract provided.

Synergistic Cancer Treatment: A Hypothetical Combination Of Several Unique Cancer Treatments, Clinton Belott

A with Honors Projects

A hypothetical treatment, which combines the effects of alpha interferon, gamma interferon, phosphatidylserine, and the vesicular stomatitis virus. (Revised May, 2011.)

Introduction, William Michael Treanor

Fordham Law Review

In view of the profound importance of questions of presidential succession and the lack of attention paid to them, this issue of the Fordham Law Review is, very simply, a great public service. It presents the papers produced by The Adequacy of the Presidential Succession System in the 21st Century Symposium, which was held at Fordham Law School on April 16 and April 17, 2010. Sitting in the audience during the Symposium and listening to the papers presented here, I was struck by how many gaps there are in our current system and what dangers those gaps pose.

It was …

Bathsheba's Dilemma: Defining, Discovering, And Defending Anglo-American Feminist Theories Of Rhetoric(S), Krista Ratcliffe

English Faculty Research and Publications

No abstract provided.

Individualization Claims In Forensic Science: Still Unwarranted, Jonathan J. Koehler, Michael J. Saks

Brooklyn Law Review

No abstract provided.