Digital Commons Network^™

A Systems Model Of Exocytic Vesicles In Arabidopsis Pollen During Its Germination, Naohiro Kato

Faculty Publications

Abasic systems model for vesicle trafficking in Arabidopsis pollen tubes was constructed. The model was composed of transcriptome data and differential equations. The transcriptome data revealed some genes controlling vesicle trafficking in the pollen tubes, and the differential equations connected the molecular functions of the gene products. The computed pollen tube growth reasonably agreed with biological samples. Here, I expand the computational prediction into exocytic vesicles during pollen germination, which can be used to examine the accuracy of the systems model with biological samples. The computational analysis of the model predicts that the number of exocytic vesicles changes in an …

Evolutionary Studies Illuminate The Structural-Functional Model Of Plant Phytochromes, Sarah Mathews

Faculty Publications

A synthesis of insights from functional and evolutionary studies reveals how the phytochrome photoreceptor system has evolved to impart both stability and flexibility. Phytochromes in seed plants diverged into three major forms, phyA, phyB, and phyC, very early in the history of seed plants. Two additional forms, phyE and phyD, are restricted to flowering plants and Brassicaceae, respectively. While phyC, D, and E are absent from at least some taxa, phyA and phyB are present in all sampled seed plants and are the principal mediators of red/far-red-induced responses. Conversely, phyC-E apparently function in concert with phyB and, where present, expand …

Nuclear Actin-Related Proteins At The Core Of Epigenetic Control, Richard B. Meagher, Muthugapatti K. Kandasamy, Aaron P. Smith, Elizabeth C. Mckinney

Faculty Publications

Nuclear Actin-Related Proteins (ARPs) and actin combine as heterodimers to bind a large helicase subunit and form a core complex essential to the assembly and function of most chromatin remodeling and modifying machines. They are the most common shared subunits of these large and diverse assemblies in eukaryotes. We recently argued that most nuclear ARPs evolved directly from actin prior to the divergence of the eukaryotic kingdoms and did not evolve from pre-existing ARPs.2 Arabidopsis plants defective in nuclear ARP4, ARP5, ARP6 or ARP7 have extreme developmental phenotypes. Our recent publication demonstrates that ARP5-defective plants are not only dwarfed and …

A Conserved Interaction Between The Sdi Domain Of Bre2 And The Dpy-30 Domain Of Sdc1 Is Required For Histone Methylation And Gene Expression, Paul F. South, Ian M. Fingerman, Douglas P. Mersman, Hai Ning Du, Scott D. Briggs

Faculty Publications

In Saccharomyces cerevisiae, lysine 4 on histone H3 (H3K4) is methylated by the Set1 complex (Set1C or COMPASS). Besides the catalytic Set1 subunit, several proteins that form the Set1C (Swd1, Swd2, Swd3, Spp1, Bre2, and Sdc1) are also needed to mediate proper H3K4 methylation. Until this study, it has been unclear how individual Set1C members interact and how this interaction may impact histone methylation and gene expression. In this study, Bre2 and Sdc1 are shown to directly interact, and it is shown that the association of this heteromeric complex is needed for proper H3K4 methylation and gene expression to occur. …

Targeted Gene Replacement By Homologous Recombination In Drosophila Stimulates Production Of Second-Site Mutations, Swarnava Roy, Craig M. Hart

Faculty Publications

Gene replacement by homologous recombination is a powerful technique for generating mutations in Drosophila. While using this technique for the BEAF gene, we encountered non-targeted lethal mutations on the target chromosome that complicated the analysis of the BEAF mutations until they were discovered and removed by meiotic recombination. Subsequent experiments indicated that the gene-targeting method leads to a modest but significant three-fold increase in the rate of production of non-targeted lethal mutations. It is important to be aware of this phenomenon when using this method. © 2010 Landes Bioscience.

Endoreplication Controls Cell Fate Maintenance, Jonathan Bramsiepe, Katja Wester, Christina Weinl, Farshad Roodbarkelari, Remmy Kasili, John C. Larkin, Martin Hülskamp, Arp Schnittger

Faculty Publications

Cell-fate specification is typically thought to precede and determine cell-cycle regulation during differentiation. Here we show that endoreplication, also known as endoreduplication, a specialized cell-cycle variant often associated with cell differentiation but also frequently occurring in malignant cells, plays a role in maintaining cell fate. For our study we have used Arabidopsis trichomes as a model system and have manipulated endoreplication levels via mutants of cell-cycle regulators and overexpression of cell-cycle inhibitors under a trichome-specific promoter. Strikingly, a reduction of endoreplication resulted in reduced trichome numbers and caused trichomes to lose their identity. Live observations of young Arabidopsis leaves revealed …

Pr10 Expression In Maize And Its Effect On Host Resistance Against Aspergillus Flavus Infection And Aflatoxin Production, Zhi Yuan Chen, Robert L. Brown, Kenneth E. Damann, Thomas E. Cleveland

Faculty Publications

Maize (Zea mays L.) is a major crop susceptible to Aspergillus flavus infection and subsequent contamination with aflatoxins, the potent carcinogenic secondary metabolites of the fungus. Protein profiles of maize genotypes resistant and susceptible to A. flavus infection and/or aflatoxin contamination have been compared, and several resistance-associated proteins have been found, including a pathogenesis-related protein 10 (PR10). In this study, RNA interference (RNAi) gene silencing technology was employed to further investigate the importance of PR10. An RNAi gene silencing vector was constructed and introduced into immature Hi II maize embryos through both bombardment and Agrobacterium infection procedures. PR10 expression was …

Development Of A Prototype Bayesian Network Model Representing The Relationship Between Fresh Market Yield And Some Agroclimatic Variables Known To Influence Storage Root Initiation In Sweetpotato, Arthur Villordon, Julio Solis, Don Labonte, Christopher Clark

Faculty Publications

A prototype phenology-driven Bayesian belief network (BBN) model, named BxNET, was developed to represent the relationship between fresh market yield (U.S. #1 grade) and agroclimatic variables known to influence the critical storage root initiation stages in 'Beauregard' sweetpotato. This data-driven model was developed from experimental data collected over 3 years of field trials in which management variables were kept as uniform as possible. The BBN was developed assuming that soil moisture measured at the 15-cm depth was not a limiting variable during the first 20 days after transplanting, during which the onset of storage root initiation determined the majority of …

Components, Volume 6 (2010), Lsu Center For Computation & Technology

Components

No abstract provided.

Chitosan On The Postharvest Control Of Soft Rot In 'Rama Forte' Persimmon, Patrícia Cia, Eliane Aparecida Benato, Sérgio Florentino Pascholati, Ely Oliveira Garcia

Faculty Publications

The objective of this research was to evaluate the effect of chitosan in addition to de-astringency process on the control of Rhizopus stolonifer in 'Rama Forte' persimmon and on in vitro mycelial growth. Persimmon were submitted to de-astringency process using CO2 (70% / 18 h), in hermetic chambers. Next, fruit were inoculated through subcuticular injections of a R. stolonifer spore suspension (3x105 spore mL-1) and 2 hours later at 25 °C immersed into chitosan (0, 0.5, 1.0, 1.5, and 2.0%), for 1 min. Fruit were stored at 25 °C / 80% RH, and checked for rot severity and incidence of …

Darwinian Populations And Natural Selection, Grant Ramsey, Hope Hollocher, Agustín Fuentes, Charles H. Pence, Edwin Siu

Faculty Publications

No abstract provided.

Computational Methods For The Analysis Of Primate Mobile Elements, Richard Cordaux, Shurjo K. Sen, Miriam K. Konkel, Mark A. Batzer

Faculty Publications

Transposable elements (TE), defined as discrete pieces of DNA that can move from one site to another site in genomes, represent significant components of eukaryotic genomes, including primates. Comparative genome-wide analyses have revealed the considerable structural and functional impact of TE families on primate genomes. Insights into these questions have come in part from the development of computational methods that allow detailed and reliable identification, annotation, and evolutionary analyses of the many TE families that populate primate genomes. Here, we present an overview of these computational methods and describe efficient data mining strategies for providing a comprehensive picture of TE …

Laboratory Methods For The Analysis Of Primate Mobile Elements, David A. Ray, Kyudong Han, Jerilyn A. Walker, Mark A. Batzer

Faculty Publications

Mobile elements represent a unique and powerful set of tools for understanding the variation in a genome. Methods exist not only to utilize the polymorphisms among and within taxa to various ends but also to investigate the mechanism through which mobilization occurs. The number of methods to accomplish these ends is ever growing. Here, we present several protocols designed to assay mobile element-based variation within and among individual genomes. © 2010 Springer Science + Business Media, LLC.

Research Matters Summer 2010, Louisiana State University And Agricultural & Mechanical College

Research Matters & RNR Newsletters

Research Matters is a publication which highlights ongoing research, international travel experiences, classroom happenings and degree program developments of and for the School of Renewable Natural Resources' students and faculty.

School Of Renewable Natural Resources Newsletter, Fall 2010, Louisiana State University And Agricultural & Mechanical College

Research Matters & RNR Newsletters

The annual newsletter from the School of Renewable Natural Resources was a publication which aimed to update and entertain interested readers with the news and research from RNR faculty, students and staff.

Teaching The Process Of Molecular Phylogeny And Systematics: A Multi-Part Inquiry-Based Exercise, Nathan H. Lents, Oscar E. Cifuentes, Anthony Carpi

Publications and Research

Three approaches to molecular phylogenetics are demonstrated to biology students as they explore molecular data from Homo sapiens and four related primates. By analyzing DNA sequences, protein sequences, and chromosomal maps, students are repeatedly challenged to develop hypotheses regarding the ancestry of the five species. Although these exercises were designed to supplement and enhance classroom instruction on phylogeny, cladistics, and systematics in the context of a postsecondary majors-level introductory biology course, the activities themselves require very little prior student exposure to these topics. Thus, they are well suited for students in a wide range of educational levels, including a biology …

The Dynamics Of Goethe's Novelle The Never-Ending Journey To Newton's Burg, Heather I. Sullivan

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

"Sickness Among The Slaves" Undermining Pathologies Of The African In Olaudah Equiano's The Interesting Narrative, Tara Czechowski

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

Phenological Partitioning Among Drifting Insect Genera Within Families In A Subtropical, Coastal Plain Stream (Louisiana, Usa), Michael D. Kaller, James D. Hudson

Faculty Publications

We investigated invertebrate drift in a subtropical, coastal plain stream in Louisiana, USA. Once monthly, at a single site on Big Brushy Creek, eight three-hour drift samples (24 hours total) were collected using two 250-micron, 0.13-m2 drift nets. We examined the data for seasonal trends in overall drift density by analysis of covariance and taxonomic composition by principal component analysis and multivariate analysis of covariance. We found no statistically significant seasonal differences in overall drift density, although community composition did statistically differ among months. More interestingly, we found that 26 genera (38%) exhibited asynchrony in peak drift timing within families. …

Sex Discrimination Under Tribal Law, Ann E. Tweedy

William Mitchell Law Review

No abstract provided.

Clean Water In Indian Country: The Risks (And Rewards) Of Being Treated In The Same Manner As A State, Marren Sanders

William Mitchell Law Review

No abstract provided.

Tropical Forest Mitigation Projects And Sustainable Development: Designing U.S. Law For A Supportive Role, Andrew Long

William Mitchell Law Review

No abstract provided.

Spreading Its Wings: Using The Migratory Bird Treaty Act To Protect Habitat, Collette L. Adkins Giese

William Mitchell Law Review

No abstract provided.

China In Context: Energy, Water, And Climate Cooperation, Elizabeth Burleson

William Mitchell Law Review

No abstract provided.

Adjusting Carbon Management Policies To Encourage Renewable, Net-Negative Projects Such As Biochar Sequestration, Darrell A. Fruth, Joseph A. Ponzi

William Mitchell Law Review

No abstract provided.

A "Good Death" Defined By Law: Comparing The Legality Of Aid-In-Dying Around The World, Kristina Ebbott

William Mitchell Law Review

No abstract provided.

The Human-Centric Warehouse, Kevin R. Gue

11th IMHRC Proceedings (Milwaukee, Wisconsin. USA – 2010)

In theory and in practice, the objective of warehouse design has long been to meet operational requirements of throughput and service performance at minimum cost. A natural product of this “bottom line” approach has been warehouse buildings and operations ill-suited to the humans who spend their working lives interacting with them. We offer an explanation for the current approach, and argue that a new approach— a new paradigm —is needed. We then describe a comprehensive approach to warehouse design that includes human well-being as a primary goal.

Core/Shell Nanoparticles As Hybrid Platforms For The Fabrication Of A Hydrogen Peroxide Biosensor, Yu-Ho Won, Doreen Aboagye, Ho Seong Jang, Andrei Jitianu, Lia A. Stanciu

Publications and Research

No abstract provided.

Over-Kill: The Ramifications Of Applying New York's Anti-Terrorism Statute Too Broadly, Louis Jim

Faculty Scholarship

No abstract provided.

Labrad : Vol 35, Issue 1 - January - February 2010, Aga Khan University Hospital, Karachi

LABRAD - Departments of Pathology & Laboratory Medicine and Radiology Archives

• Assessment of Osteoporosis Risk by NTx- A Biochemical Marker
• Antibodies Against Cyclic Citrullinated Peptides (Anti-CCP) Assay in Rheumatoid Arthritis
• A Disintegrin and Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS-13)
• Soluble Transferrin Receptor (sTfR)
• Diagnosis of Cryptococcal Meningitis
• Infections in Thalassaemia
• CDX-2, A Reliable Diagnostic Marker for Carcinomas of Colorectal Origin
• Radiopaque Metallic Marker Insertion in Breast Cancers Prior to Neoadjuvant/Preoperative Chemotherapy
• Meeting Reports: 10TH International Radiological Conference 2009
• Continuing Medical Education Seminar Organised by Clinical Laboratory at AKUH