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Articles 4831 - 4860 of 5262
Full-Text Articles in Entire DC Network
Responders And Non-Responders To Probiotic Interventions, Gregor Reid, Estelle Gaudier, Francisco Guarner, Gary B. Huffnagle, Jean M. Macklaim, Alicia Murcia Munoz, Margaret Martini, Tamar Ringel-Kulka, Balfor R. Sartor, Robert R. Unal, Kristin Verbeke, Jens Walter
Responders And Non-Responders To Probiotic Interventions, Gregor Reid, Estelle Gaudier, Francisco Guarner, Gary B. Huffnagle, Jean M. Macklaim, Alicia Murcia Munoz, Margaret Martini, Tamar Ringel-Kulka, Balfor R. Sartor, Robert R. Unal, Kristin Verbeke, Jens Walter
Department of Food Science and Technology: Faculty Publications
As with many clinical studies, trials using probiotics have shown clearly that some patients benefit from the treatment while others do not. For example if treatment with probiotics leads to 36% cure rate of diarrhea, why did the other 64% not have the same result? The issue is important for human and indeed experimental animal studies for two main reasons: (i) Would changing the design of the study result in more subjects responding to treatment? (ii) If a subject does not respond what are the mechanistic reasons? In order to tackle the issue of responders and non-responders to therapy, a …
Migration Of Northern Yellowstone Elk: Implications Of Spatial Structuring, P. J. White, Kelly M. Proffitt, L. David Mech, Shaney B. Evans, Julie A. Cunningham, Kenneth L. Hamlin
Migration Of Northern Yellowstone Elk: Implications Of Spatial Structuring, P. J. White, Kelly M. Proffitt, L. David Mech, Shaney B. Evans, Julie A. Cunningham, Kenneth L. Hamlin
United States Geological Survey, Northern Prairie Wildlife Research Center: Publications
Migration can enhance survival and recruitment of mammals by increasing access to higher-quality forage or reducing predation risk, or both. We used telemetry locations collected from 140 adult female elk during 2000– 2003 and 2007–2008 to identify factors influencing the migration of northern Yellowstone elk. Elk wintered in 2 semidistinct herd segments and migrated 10–140 km to at least 12 summer areas in Yellowstone National Park (YNP) and nearby areas of Montana. Spring migrations were delayed after winters with increased snow pack, with earlier migration in years with earlier vegetation green-up. Elk wintering at lower elevations outside YNP migrated an …
Proportion Of Calves And Adult Muskoxen, Ovibos Moschatus Killed By Gray Wolves, Canis Lupus, In July On Ellesmere Island, L. David Mech
Proportion Of Calves And Adult Muskoxen, Ovibos Moschatus Killed By Gray Wolves, Canis Lupus, In July On Ellesmere Island, L. David Mech
United States Geological Survey, Northern Prairie Wildlife Research Center: Publications
Generally Gray Wolves (Canis lupus L., 1758) tend to focus predation on young-of-the-year ungulates during summer, and I hypothesized that wolves preying on Muskoxen (Ovibos moschatus Zimmerman, 1780) in summer would follow that trend. Over 23 July periods observing wolves on Ellesmere Island, Nunavut, Canada, I found that packs of 2-12 adult wolves killed seven calves, one yearling, and five adult muskoxen at distances of 2.9 to 32 km from their current dens and pups. Given a possible bias against finding calves because of their fewer remains, these results do not necessarily refute the hypothesis, but they do …
Neuroaids In Africa, Kevin Robertson, Jeff Liner, James Hakim, Jean-Louis Sankalé, Igor Grant, Scott Letendre, David Clifford, Amadou Gallo Diop, Assan Jaye, Georgette Kanmogne, Alfred Njamnshi, T. Dianne Langford, Tufa Gemechu Weyessa, Charles Wood, Mwanza Banda, Mina Hosseinipour, Ned Sacktor, Noeline Nakasuja, Paul Bangirana, Robert Paul, John Joska, Joseph Wong, Michael Boivin, Penny Holding, Betsy Kammerer, Annelies Van Rie, Prudence Ive, Avindra Nath, Kathy Lawler, Clement Adebamowo, Walter Royal Iii, Jeymohan Joseph
Neuroaids In Africa, Kevin Robertson, Jeff Liner, James Hakim, Jean-Louis Sankalé, Igor Grant, Scott Letendre, David Clifford, Amadou Gallo Diop, Assan Jaye, Georgette Kanmogne, Alfred Njamnshi, T. Dianne Langford, Tufa Gemechu Weyessa, Charles Wood, Mwanza Banda, Mina Hosseinipour, Ned Sacktor, Noeline Nakasuja, Paul Bangirana, Robert Paul, John Joska, Joseph Wong, Michael Boivin, Penny Holding, Betsy Kammerer, Annelies Van Rie, Prudence Ive, Avindra Nath, Kathy Lawler, Clement Adebamowo, Walter Royal Iii, Jeymohan Joseph
Nebraska Center for Virology: Faculty Publications
In July 2009, the Center for Mental Health Research on AIDS at the National Institute of Mental Health organized and supported the meeting “NeuroAIDS in Africa.” This meeting was held in Cape Town, South Africa, and was affiliated with the 5th IAS Conference on HIV Pathogenesis, Treatment and Prevention. Presentations began with an overview of the epidemiology of HIV in sub-Saharan Africa, the molecular epidemiology of HIV, HIV-associated neurocognitive disorders (HANDs), and HAND treatment. These introductory talks were followed by presentations on HAND research and clinical care in Botswana, Cameroon, Ethiopia, The Gambia, Kenya, Malawi, Nigeria, Senegal, South Africa, Uganda, …
Enhancement Of Autophagy During Lytic Replication By The Kaposi’S Sarcoma-Associated Herpesvirus Replication And Transcription Activator, Hui-Ju Wen, Zhilong Yang, You Zhou, Charles Wood
Enhancement Of Autophagy During Lytic Replication By The Kaposi’S Sarcoma-Associated Herpesvirus Replication And Transcription Activator, Hui-Ju Wen, Zhilong Yang, You Zhou, Charles Wood
Nebraska Center for Virology: Faculty Publications
Autophagy is one of two major degradation systems in eukaryotic cells. The degradation mechanism of autophagy is required to maintain the balance between the biosynthetic and catabolic processes and also contributes to defense against invading pathogens. Recent studies suggest that a number of viruses can evade or subvert the host cell autophagic pathway to enhance their own replication. Here, we investigated the effect of autophagy on the KSHV (Kaposi’s sarcoma-associated herpesvirus) life cycle. We found that the inhibition of autophagy reduces KSHV lytic reactivation from latency, and an enhancement of autophagy can be detected during KSHV lytic replication. In addition, …
Fine-Scale Genetic And Social Structuring In A Central Appalachian White-Tailed Deer Herd, Brad F. Miller, Randy W. Deyoung, Tyler A. Campbell, Benjamin R. Laseter, W. Mark Ford, Karl V. Miller
Fine-Scale Genetic And Social Structuring In A Central Appalachian White-Tailed Deer Herd, Brad F. Miller, Randy W. Deyoung, Tyler A. Campbell, Benjamin R. Laseter, W. Mark Ford, Karl V. Miller
United States Department of Agriculture Wildlife Services: Staff Publications
Spatial genetic structure in white-tailed deer (Odocoileus virginianus) has been examined at regional scales, but genetic markers with the resolution to detect fine-scale patterns have appeared only recently. We used a panel of microsatellite DNA markers, radiotelemetry data, and visual observations of marked deer to study fine-scale social and genetic structure in a high-density population of white-tailed deer (12–20 deer/km2). We collected genetic data on 229 adult females, 102 of which were assigned to 28 social groups. Our results were consistent with the conceptual model of white-tailed deer social structure, where philopatric females form social groups …
Review Of Issues Concerning The Use Of Reproductive Inhibitors, With Particular Emphasis On Resolving Human-Wildlife Conflicts In North America, Kathleen A. Fagerstone, Lowell A. Miller, Gary Killian, Christi A. Yoder
Review Of Issues Concerning The Use Of Reproductive Inhibitors, With Particular Emphasis On Resolving Human-Wildlife Conflicts In North America, Kathleen A. Fagerstone, Lowell A. Miller, Gary Killian, Christi A. Yoder
United States Department of Agriculture Wildlife Services: Staff Publications
This manuscript provides an overview of past wildlife contraception efforts and discusses the current state of research. Two fertility control agents, an avian reproductive inhibitor containing the active ingredient nicarbazin and an immunocontraceptive vaccine, have received regulatory approval with the Environmental Protection Agency and are commercially available in the USA. OvoControl G Contraceptive Bait for Canada Geese and Ovo Control for pigeons are delivered as oral baits. An injectable immunocontraceptive vaccine (GonaCon Immunocontraceptive Vaccine) was registered with the Environmental Protection Agency for use in female white-tailed deer in September 2009. An injectable product (GonaCon Immunocontraceptive Vaccine) is registered for use …
Emissions Savings In The Corn-Ethanol Life Cycle From Feeding Coproducts To Livestock, Virgil R. Bremer, Adam Liska, Terry J. Klopfenstein, Galen E. Erickson, Haishun Yang, Daniel T. Walters, Kenneth G. Cassman
Emissions Savings In The Corn-Ethanol Life Cycle From Feeding Coproducts To Livestock, Virgil R. Bremer, Adam Liska, Terry J. Klopfenstein, Galen E. Erickson, Haishun Yang, Daniel T. Walters, Kenneth G. Cassman
Department of Agronomy and Horticulture: Faculty Publications
Environmental regulations on greenhouse gas (GHG) emissions from corn (Zea mays L.)-ethanol production require accurate assessment methods to determine emissions savings from coproducts that are fed to livestock. We investigated current use of coproducts in livestock diets and estimated the magnitude and variability in the GHG emissions credit for coproducts in the corn-ethanol life cycle. The coproduct GHG emissions credit varied by more than twofold, from 11.5 to 28.3 g CO2e per MJ of ethanol produced, depending on the fraction of coproducts used without drying, the proportion of coproduct used to feed beef cattle (Bos taurus …
Effect Of Nitrogen Addition And Weed Interference On Soil Nitrogen And Corn Nitrogen Nutrition, John L. Lindquist, Sean P. Evans, Charles A. Shapiro, Stevan Z. Knezevic
Effect Of Nitrogen Addition And Weed Interference On Soil Nitrogen And Corn Nitrogen Nutrition, John L. Lindquist, Sean P. Evans, Charles A. Shapiro, Stevan Z. Knezevic
Department of Agronomy and Horticulture: Faculty Publications
Weeds cause crop loss indirectly by reducing the quantity of resources available for growth. Quantifying the effects of weed interference on nitrogen (N) supply, crop growth, and N nutrition may assist in making both N and weed management decisions. Experiments were conducted to quantify the effect of N addition and weed interference on soil nitrate-N (NO3- N) over time and the dependence of corn growth on NO3-N availability, determine the corn N nutrition index (NNI) at anthesis, and evaluate if relative chlorophyll content can be utilized as a reliable predictor of NNI. Urea was applied at 0, 60, …
Rna-Seq Atlas Of Glycine Max: A Guide To The Soybean Transcriptome, Andrew J. Severin, Jenna L. Woody, Yung-Tsi Bolon, Bindu Joseph, Brian W. Diers, Andrew Farmer, Gary J. Muehlbauer, Rex T. Nelson, David Grant, James E. Specht, Michelle A. Graham, Steven B. Cannon, Gregory D. May, Carroll P. Vance, Randy C. Shoemaker
Rna-Seq Atlas Of Glycine Max: A Guide To The Soybean Transcriptome, Andrew J. Severin, Jenna L. Woody, Yung-Tsi Bolon, Bindu Joseph, Brian W. Diers, Andrew Farmer, Gary J. Muehlbauer, Rex T. Nelson, David Grant, James E. Specht, Michelle A. Graham, Steven B. Cannon, Gregory D. May, Carroll P. Vance, Randy C. Shoemaker
Department of Agronomy and Horticulture: Faculty Publications
Background: Next generation sequencing is transforming our understanding of transcriptomes. It can determine the expression level of transcripts with a dynamic range of over six orders of magnitude from multiple tissues, developmental stages or conditions. Patterns of gene expression provide insight into functions of genes with unknown annotation.
Results: The RNA Seq-Atlas presented here provides a record of high-resolution gene expression in a set of fourteen diverse tissues. Hierarchical clustering of transcriptional profiles for these tissues suggests three clades with similar profiles: aerial, underground and seed tissues. We also investigate the relationship between gene structure and gene expression and find …
Economic Potential Of Substituting Legumes For Synthetic Nitrogen In Warm Season Perennial Grasses Used For Stocker Cattle Grazing, Maru Kering, John Biermacher, Ryan Reuter, Twain Butler, Job D. Springer, James Rogers, John Blanton, Joe Bouton, John A. Guretzky
Economic Potential Of Substituting Legumes For Synthetic Nitrogen In Warm Season Perennial Grasses Used For Stocker Cattle Grazing, Maru Kering, John Biermacher, Ryan Reuter, Twain Butler, Job D. Springer, James Rogers, John Blanton, Joe Bouton, John A. Guretzky
Department of Agronomy and Horticulture: Faculty Publications
Stocker cattle grazing warm season perennial grasses is an important economic activity in the southern Great Plains. Substantial increases in the price of nitrogen fertilizer is negatively affecting forage producers‟ profitability. Two alternative nitrogen management systems that use annual and perennial legumes have been developed for bermudagrass pastures. The goal of the study is to determine if the legumes systems are more profitable than the conventional practice of applying synthetic sources of nitrogen. Results of the two-year grazing study show that the legume systems could not compete economically with the common practice.
Fertilizer Rate Effects On Forage Yield Stability And Nutrient Uptake Of Midland Bermudagrass, John A. Guretzky, Maru K. Kering, Jagadeesh Mosali, Eddie Funderburg, Jon T. Biermacher
Fertilizer Rate Effects On Forage Yield Stability And Nutrient Uptake Of Midland Bermudagrass, John A. Guretzky, Maru K. Kering, Jagadeesh Mosali, Eddie Funderburg, Jon T. Biermacher
Department of Agronomy and Horticulture: Faculty Publications
Our objectives were to document effects of nitrogen (N), phosphorus (P), and potassium (K) fertilizer rates on forage yields and uptake of N, P, and K by Midland bermudagrass [Cynodon dactylon (L.) Pers.] on a Minco fine, sandy loam in southern Oklahoma. After six years of this long-term experiment, forage yield responses to fertilization were mixed and depended on year. Stability analysis indicated forage yields responded positively to N fertilization during favorable weather conditions but negatively during poor weather conditions. Application of 112 kg N ha−1 provided the best yield stability and mean annual forage yield among treatments, …
Evaluation Of The Intercom Model For Predicting Growth Of Forest Herbs, Zorica S. Popović, J. L. Lindquist
Evaluation Of The Intercom Model For Predicting Growth Of Forest Herbs, Zorica S. Popović, J. L. Lindquist
Department of Agronomy and Horticulture: Faculty Publications
The ecophysiological model INTERCOM was evaluated for its suitability in predicting the growth of perennial forest herbs. A field experiment was conducted to obtain data on photosynthesis and growth parameters of two spring flowering understorey geophytes. Results were used to parameterize the model and its performance was evaluated using the average normalized difference (AE) between predicted and observed biomass and the leaf area index. The model was assumed to provide accurate simulations if the AE was smaller than 0.4. Adjusting the photosynthetic intensity parameters in the model to reflect observed changes in photosynthesis throughout the growing period resulted in the …
High-Throughput Snp Discovery And Assay Development In Common Bean, David L. Hyten, Qijian Song, Edward W. Fickus, Charles V. Quigley, Jong-Sung Lim, Ik-Young Choi, Eun-Young Hwang, Marcial Pastor-Corrales, Perry B. Cregan
High-Throughput Snp Discovery And Assay Development In Common Bean, David L. Hyten, Qijian Song, Edward W. Fickus, Charles V. Quigley, Jong-Sung Lim, Ik-Young Choi, Eun-Young Hwang, Marcial Pastor-Corrales, Perry B. Cregan
Department of Agronomy and Horticulture: Faculty Publications
Background: Next generation sequencing has significantly increased the speed at which single nucleotide polymorphisms (SNPs) can be discovered and subsequently used as molecular markers for research. Unfortunately, for species such as common bean (Phaseolus vulgaris L.) which do not have a whole genome sequence available, the use of next generation sequencing for SNP discovery is much more difficult and costly. To this end we developed a method which couples sequences obtained from the Roche 454-FLX system (454) with the Illumina Genome Analyzer (GA) for high-throughput SNP discovery. Results: Using a multi-tier reduced representation library we discovered a total of …
Modeling Changes In Biomass Composition During Microwave-Based Alkali Pretreatment Of Switchgrass, Deepak R. Keshwani, Jay J. Cheng
Modeling Changes In Biomass Composition During Microwave-Based Alkali Pretreatment Of Switchgrass, Deepak R. Keshwani, Jay J. Cheng
Department of Agricultural and Biological Systems Engineering: Faculty Publications
This study used two different approaches to model changes in biomass composition during microwave-based pretreatment of switchgrass: kinetic modeling using a time-dependent rate coefficient, and a Mamdani-type fuzzy inference system. In both modeling approaches, the dielectric loss tangent of the alkali reagent and pretreatment time were used as predictors for changes in amounts of lignin, cellulose, and xylan during the pretreatment. Training and testing data sets for development and validation of the models were obtained from pretreatment experiments conducted using 1–3% w/v NaOH (sodium hydroxide) and pretreatment times ranging from 5 to 20 minutes. The kinetic modeling approach for lignin …
In Vitro Stable Isotope Labeling For Discovery Of Novel Metabolites By Liquid Chromatography-Mass Spectrometry: Confirmation Of Γ-Tocopherol Metabolism In Human A549 Cell, Wen-Chu Yang, Fred E. Regnier, Qing Jiang, Jiri Adamec
In Vitro Stable Isotope Labeling For Discovery Of Novel Metabolites By Liquid Chromatography-Mass Spectrometry: Confirmation Of Γ-Tocopherol Metabolism In Human A549 Cell, Wen-Chu Yang, Fred E. Regnier, Qing Jiang, Jiri Adamec
Department of Biochemistry: Faculty Publications
A general approach for discovering novel catabolic metabolites from a parent biocompound was developed and validated on metabolism of γ-tocopherol in human A549 cell. Method is based on LC-MS analysis of in vitro stable isotope labeled metabolites and assumes that a parent compound and its metabolites share a common functional group that can be derivatized by well-documented reagents. In this method, two equal aliquots of extracted metabolites are separately derivatized with isotope-coded (heavy) and non-isotope-coded (light) form of derivatizing reagent, mixed at 1:1 ratio and analyzed using LC-MS. The metabolites with common functional group are then easily recognized by determination …
Quantitative Nuclear Proteomics Identifies Mtor Regulation Of Dna Damage Response, Sricharan Bandhakavi, Young-Mi Kim, Seung-Hyun Ro, Hongwei Xie, Getiria Onsongo, Chang-Bong Jun, Do-Hyung Kim, Timothy J. Griffin
Quantitative Nuclear Proteomics Identifies Mtor Regulation Of Dna Damage Response, Sricharan Bandhakavi, Young-Mi Kim, Seung-Hyun Ro, Hongwei Xie, Getiria Onsongo, Chang-Bong Jun, Do-Hyung Kim, Timothy J. Griffin
Department of Biochemistry: Faculty Publications
Cellular nutritional and energy status regulates a wide range of nuclear processes important for cell growth, survival, and metabolic homeostasis. Mammalian target of rapamycin (mTOR) plays a key role in the cellular responses to nutrients. However, the nuclear processes governed by mTOR have not been clearly defined. Using isobaric peptide tagging coupled with linear ion trap mass spectrometry, we performed quantitative proteomics analysis to identify nuclear processes in human cells under control of mTOR. Within 3 h of inhibiting mTOR with rapamycin in HeLa cells, we observed downregulation of nuclear abundance of many proteins involved in translation and RNA modification. …
Bioassays Of Compounds With Potential Juvenoid Activity On Drosophila Melanogaster: Juvenile Hormone Iii, Bisepoxide Juvenile Hormone Iii, And Methyl Farnesoates, Lawrence G. Harshman, Ki-Duck Song, Josephina Casas, A. Schuurmans, Eichii Kuwano, Stephen D. Kachman, Lynn M. Riddiford, Bruce D. Hammock
Bioassays Of Compounds With Potential Juvenoid Activity On Drosophila Melanogaster: Juvenile Hormone Iii, Bisepoxide Juvenile Hormone Iii, And Methyl Farnesoates, Lawrence G. Harshman, Ki-Duck Song, Josephina Casas, A. Schuurmans, Eichii Kuwano, Stephen D. Kachman, Lynn M. Riddiford, Bruce D. Hammock
Lawrence G. Harshman Publications
Metabolites of the 6,7,10,11 bisepoxide juvenile hormone III (JHB3), and other potential juvenoids, were tested for juvenile hormone activity using early instar or early stage pupae of Drosophila mela-nogaster. Importantly, methyl farnesoates were tested as they might have JH-like activity on Dipteran juveniles. Larvae were exposed to compounds in medium, or the compounds were applied to white puparia. In the assays employed in the present study, there was no indication for JH activity associ-ated with the metabolites of JHB3. The activity of methyl farnesoate (MF) was higher than that of JH III and far greater …
Multimodal Courtship Efficacy Of Schizocosa Retrorsa Wolf Spiders: Implications Of An Additional Signal Modality, Aaron S. Rundus, Roger D. Santer, Eileen Hebets
Multimodal Courtship Efficacy Of Schizocosa Retrorsa Wolf Spiders: Implications Of An Additional Signal Modality, Aaron S. Rundus, Roger D. Santer, Eileen Hebets
Eileen Hebets Publications
Here, we simultaneously examine both content and efficacy-based sources of selection on the visual and seismic multimodal courtship display of the wolf spider Schizocosa retrorsa. Immature field-collected S. retrorsa were reared in the laboratory on either high-quantity diet (HD) or low-quantity diet (LD) treatments. On maturation, females of each diet treatment were run in simultaneous mate choice trials with both a HD and an LD male (content-based selection). Simultaneous mate choice trials were conducted across different signaling environments (efficacy-based selection) in a fully crossed 2 × 2 design with visual treatments of light/dark (visual signal present/absent) and seismic treatments …
Evolutionary Aspects Of Urea Utilization By Fungi, Dhammika H. M. L. P Navarathna, Steven D. Harris, David D. Roberts, Kenneth W. Nickerson
Evolutionary Aspects Of Urea Utilization By Fungi, Dhammika H. M. L. P Navarathna, Steven D. Harris, David D. Roberts, Kenneth W. Nickerson
Kenneth Nickerson Papers
The higher fungi exhibit a dichotomy with regard to urea utilization. The hemiascomycetes use urea amidolyase (DUR1,2), whereas all other higher fungi use the nickel-containing urease. Urea amidolyase is an energy-dependent biotincontaining enzyme. It likely arose before the Euascomycete/Hemiascomycete divergence c. 350 million years ago by insertion of an unknown gene into one copy of a duplicated methylcrotonyl CoA carboxylase (MccA). The dichotomy between urease and urea amidolyase coincides precisely with that for the Ni/Co transporter (Nic1p), which is present in the higher fungi that use urease and is absent in those that do not. We suggest that …
Soil-Transmitted Helminthiases: Implications Of Climate Change And Human Behavior, Haylee J. Weaver, John M. Hawdon, Eric P. Hoberg
Soil-Transmitted Helminthiases: Implications Of Climate Change And Human Behavior, Haylee J. Weaver, John M. Hawdon, Eric P. Hoberg
Harold W. Manter Laboratory of Parasitology: Faculty Publications
Soil-transmitted helminthiases (STHs) collectively cause the highest global burden of parasitic disease after malaria and are most prevalent in the poorest communities, especially in sub-Saharan Africa. Climate change is predicted to alter the physical environment through cumulative impacts of warming and extreme fluctuations in temperature and precipitation, with cascading effects on human health and wellbeing, food security and socioeconomic infrastructure. Understanding how the spectrum of climate change effects will influence STHs is therefore of critical importance to the control of the global burden of human parasitic disease. Realistic progress in the global control of STH in a changing climate requires …
Using Nmr Metabolomics To Investigate Tricarboxylic Acid Cycle-Dependent Signal Transduction In Staphylococcus Epidermidis, Marat R. Sadykov, Bo Zhang, Steven M. Halouska, Jennifer L. Nelson, Lauren W. Kreimer, Yefei Zhu, Robert Powers, Greg A. Somerville
Using Nmr Metabolomics To Investigate Tricarboxylic Acid Cycle-Dependent Signal Transduction In Staphylococcus Epidermidis, Marat R. Sadykov, Bo Zhang, Steven M. Halouska, Jennifer L. Nelson, Lauren W. Kreimer, Yefei Zhu, Robert Powers, Greg A. Somerville
Robert Powers Publications
Staphylococcus epidermidis is a skin-resident bacterium and a major cause of biomaterial-associated infections. The transition from residing on the skin to residing on an implanted biomaterial is accompanied by regulatory changes that facilitate bacterial survival in the new environment. These regulatory changes are dependent upon the ability of bacteria to “sense” environmental changes. In S. epidermidis, disparate environmental signals can affect synthesis of the biofilm matrix polysaccharide intercellular adhesin (PIA). Previously, we demonstrated that PIA biosynthesis is regulated by tricarboxylic acid (TCA) cycle activity. The observations that very different environmental signals result in a common phenotype (i.e. increased PIA …
Purification Of Parvalbumin From Carp: A Protocol That Avoids Heat Treatment, Stef J. Koppelman, Roland A. Romijn, Harmen H. J. De Jongh, Julie A. Nordlee, Sander Piersma, Martin Hessing, Steve L. Taylor
Purification Of Parvalbumin From Carp: A Protocol That Avoids Heat Treatment, Stef J. Koppelman, Roland A. Romijn, Harmen H. J. De Jongh, Julie A. Nordlee, Sander Piersma, Martin Hessing, Steve L. Taylor
Department of Food Science and Technology: Faculty Publications
Parvalbumin from carp, a major allergen,was purified to homogeneity using ion exchange chromatography and size exclusion chromatography (estimated purity > 95% to 98% based on SDS-PAGE and native PAGE) with a yield of 318 mg, and a number of basic biochemical characteristics were determined. The identity was confirmed by peptide-mass fingerprinting, and IgE-binding was demonstrated. The UV/Vis absorbance spectra were explained using the previously published amino acid sequences. Far UV-CD spectroscopy was used to confirm the folding character of parvalbumin. We conclude that parvalbumin from carp can be purified on a comparatively large (hundreds of milligrams) scale using a purification protocol …
Is Long-Term Hunger (Ramadan Model) A Risk Factor For Acute Appendicitis?, Barlas Sulu, Yusuf Gunerhan, Baris Ozturk, Hasan Arslan
Is Long-Term Hunger (Ramadan Model) A Risk Factor For Acute Appendicitis?, Barlas Sulu, Yusuf Gunerhan, Baris Ozturk, Hasan Arslan
Saudi Medical Journal
OBJECTIVE: To investigate whether long-term hunger and the changes in nutritional routines during Ramadan constitute risk factors in acute appendicitis on the model of Ramadan fasting in this study. METHODS: This retrospective study was carried out in 2 different hospitals (Istanbul Haydarpasa Numune Training and Research Hospital and Kars State Hospital, Kars, Turkey) between January 2004 and December 2007. The data obtained from the patients were classified according to age, gender, age group, and pathological characteristics of the appendix. The data obtained during the Ramadan fasting period was compared with the periods before and after. RESULTS: Nine hundred and ninety-two …
Vitamin D Status In Health Care Professionals In Qatar, Salah Mahdy, Samar A. Al-Emadi, Izzat A. Khanjar, Mohammed M. Hammoudeh, Housam A. Sarakbi, Abdulrahim M. Siam, Mohammed Osman Abdelrahman
Vitamin D Status In Health Care Professionals In Qatar, Salah Mahdy, Samar A. Al-Emadi, Izzat A. Khanjar, Mohammed M. Hammoudeh, Housam A. Sarakbi, Abdulrahim M. Siam, Mohammed Osman Abdelrahman
Saudi Medical Journal
OBJECTIVE: To investigate the prevalence of vitamin D deficiency among health care professionals working at Hamad Medical Corporation in Doha, Qatar. METHODS: Between 15th January 2007 and 15th January 2008, 340 healthy volunteers were included in this study. Each subject completed a diary to determine the duration of sunlight exposure, and vitamin D supplements. Serum levels of 25-hydroxyvitamin D (25OHD), parathyroid hormone (PTH), calcium, phosphorus, alkaline phosphatase, total protein, and albumin were obtained. Those with abnormal results were called for counselling. RESULTS: The mean overall vitamin D level was 11.7 ng/ml. It was lower in females (10.3 ng/ml) than in …
Characteristics Of Women Attending A Urogynecology Clinic In Riyadh, Ahmed H. Al-Badr, Aneela G. Kamil, Ehab F. Wahbah, Valerie A. Zimmerman, Mohamad A. Al-Tannir
Characteristics Of Women Attending A Urogynecology Clinic In Riyadh, Ahmed H. Al-Badr, Aneela G. Kamil, Ehab F. Wahbah, Valerie A. Zimmerman, Mohamad A. Al-Tannir
Saudi Medical Journal
No abstract provided.
The Polypeptide Syn67 Interacts Physically With Human Holocarboxylase Synthetase, But Is Not A Target For Biotinylation, Yousef I. Hassan, Hideaki Moriyama, Janos Zempleni
The Polypeptide Syn67 Interacts Physically With Human Holocarboxylase Synthetase, But Is Not A Target For Biotinylation, Yousef I. Hassan, Hideaki Moriyama, Janos Zempleni
School of Biological Sciences: Faculty Publications
Holocarboxylase synthetase (HCS) catalyzes the binding of biotin to lysines in carboxylases and histones in two steps. First, HCS catalyzes the synthesis of biotinyl-5′-AMP; second, the biotinyl moiety is ligated to lysine residues. It has been proposed that step two is fairly promiscuous, and that protein biotinylation may occur in the absence of HCS as long as sufficient exogenous biotinyl-5′- AMP is provided. Here, we identified a novel polypeptide (Syn67) with a basic patch of lysines and arginines. Yeast-two-hybrid assays and limited proteolysis assays revealed that both N- and C-termini of HCS interact with Syn67. A potential target lysine in …
Temporal Variation In Terrestrial Invertebrate Consumption By Laughing Gulls In New York, Glen E. Bernhardt, Lisa Kutschbach-Brohl, Brian E. Washburn, Richard Chipman, Laura Francoeur
Temporal Variation In Terrestrial Invertebrate Consumption By Laughing Gulls In New York, Glen E. Bernhardt, Lisa Kutschbach-Brohl, Brian E. Washburn, Richard Chipman, Laura Francoeur
United States Department of Agriculture Wildlife Services: Staff Publications
Laughing gulls (Larus atricilla) are commonly found in many areas of North America and little is known about their diet, particularly in coastal-urban interfaces where gull-aircraft collisions can be a serious concern. The objective of this study was to describe and quantify the consumption of terrestrial invertebrates by laughing gulls at a coastal-urban interface in the northeastern United States. We examined the stomach contents of laughing gulls (n = 1053) collected during wildlife damage management operations at John F. Kennedy International Airport during the summers of 2003 and 2004. Terrestrial invertebrates consumed by laughing gulls represented 2 taxonomic …
Review Of Issues Concerning The Use Of Reproductive Inhibitors, With Particular Emphasis On Resolving Human-Wildlife Conflicts In North America, Kathleen A. Fagerstone, Lowell A. Miller, Gary Killian, Christi A. Yoder
Review Of Issues Concerning The Use Of Reproductive Inhibitors, With Particular Emphasis On Resolving Human-Wildlife Conflicts In North America, Kathleen A. Fagerstone, Lowell A. Miller, Gary Killian, Christi A. Yoder
United States Department of Agriculture Wildlife Services: Staff Publications
This manuscript provides an overview of past wildlife contraception efforts and discusses the current state of research. Two fertility control agents, an avian reproductive inhibitor containing the active ingredient nicarbazin and an immunocontraceptive vaccine, have received regulatory approval with the Environmental Protection Agency and are commercially available in the USA. OvoControl G Contraceptive Bait for Canada Geese and Ovo Control for pigeons are delivered as oral baits. An injectable immunocontraceptive vaccine (GonaCon Immunocontraceptive Vaccine) was registered with the Environmental Protection Agency for use in female white-tailed deer in September 2009. An injectable product (GonaCon Immunocontraceptive Vaccine) is registered for use …
Dramatic And Immediate Improvements In Insular Nesting Success For Threatened Sea Turtles And Shorebirds Following Predator Management, Richard M. Engeman, Anthony Duffiney, Sally Braem, Christina Olsen, Bernice Constantin, Parks Small, John Dunlap, J.C. Griffin
Dramatic And Immediate Improvements In Insular Nesting Success For Threatened Sea Turtles And Shorebirds Following Predator Management, Richard M. Engeman, Anthony Duffiney, Sally Braem, Christina Olsen, Bernice Constantin, Parks Small, John Dunlap, J.C. Griffin
United States Department of Agriculture Wildlife Services: Staff Publications
Predation critically threatens reproductive success of sea turtles and shorebirds at many of Florida's beaches. We examined the biological and bioeconomic results of predator management on two adjacent barrier islands, Cayo Costa and North Captiva, along Florida's west coast. Both islands suffered severe nesting losses due to predation and disturbance due to raccoons, while Cayo Costa also was impacted by a large population of feral swine. In 2006, our initial year of study, neither island received predator management and no least tern production occurred on either island, and sea turtle nest predation was 74% and 60%, respectively, for Cayo Costa …