Digital Commons Network^™

Successful Treatment Of Vancomycin-Intermediate Staphylococcus Aureus Pacemaker Lead Infective Endocarditis With Telavancin, Luis A. Marcos, Bernard C. Camins

Open Access Publications

No abstract provided.

Comparison Of The Eragen Multi-Code Respiratory Virus Panel With Conventional Viral Testing And Real-Time Multiplex Pcr Assays For Detection Of Respiratory Viruses, Max Q. Arens, Richard S. Buller, Anne Rankin, Sheila Mason, Amy Whetsell, Eugene Agapov, Wai-Ming Lee, Gregory A. Storch

Open Access Publications

No abstract provided.

Variation In Streptococcus Pyogenes Nad+ Glycohydrolase Is Associated With Tissue Tropism, David J. Riddle, Debra E. Bessen, Michael G. Caparon

Open Access Publications

No abstract provided.

Enterococcal Biofilm Formation And Virulence In An Optimized Murine Model Of Foreign Body-Associated Urinary Tract Infections, Pascale S. Guiton, Chia S. Hung, Lynn E. Hancock, Michael G. Caparon, Scott J. Hultgren

Open Access Publications

No abstract provided.

Virulence Plasmid Harbored By Uropathogenic Escherichia Coli Functions In Acute Stages Of Pathogenesis, Corinne K. Cusumano, Chia S. Hung, Swaine L. Chen, Scott J. Hultgren

Open Access Publications

No abstract provided.

Introduction: Mjlst At 5 Years, The Consortium At 10, Susan M. Wolf

Minnesota Journal of Law, Science & Technology

No abstract provided.

Faculty Masthead, Mjlst Editorial Board

Minnesota Journal of Law, Science & Technology

No abstract provided.

Characterization Of Erythrocyte Receptor Polymorphisms In A Malaria Endemic Population In Kilifi, Kenya, Irene Akinyi Omedo

All Master’s Theses

Malaria is a strong selective force in the human genome, selecting genes for resistance to disease in human populations living in malaria endemic areas. Selection by malaria has generated genetic variations, providing evolutionary driving force mediating polymorphisms such as Glucose-6-Phosphate Dehydrogenase deficiency and sickle-cell anaemia. Genes encoding erythrocyte receptors of Plasmodium falciparum especially the sialoglycoproteins, glycophorins A, B and C, which are the main parasite receptors, have been shown to be under selection, with Single Nucleotide Polymorphisms (SNPs) and exon deletions being detected. This study analyzed SNPs in glycophorins A, B, and C genes in a population living in the …

Relative Attractiveness Of Anopheles Gambiae Mosquitoes To Synthetic Oduor Blends And Human Emanations, Evelyn Adhiambo Olanga

All Master’s Theses

Mosquito host seeking behaviour is mediated by chemical, physical and visual cues. Identification of chemical and physical cues utilized by mosquitoes in host location can lead to the development of novel vector management strategies. This research set out to identify synthetic odour blends that simulate human body emanations that can be used to develop mass trapping devices for vector management. The relative attractiveness of human body emanations and synthetic odour blends as attractants of Anopheles gambiae mosquitoes was investigated under semi-field conditions at the International Centre for Insect Physiology and Ecology (ICIPE) Mbita Point, western Kenya. The effect of heat …

Entomopathogenicity Of Hyphomycete Fungi To Fruit Fly Bactrocera Invadens (Diptera: Tephritidae) And Their Potential For Biological Control On Mango, Elizabeth Awuor Ouna

All Master’s Theses

Sustainable mango production will rely increasingly on alternatives to conventional chemical insecticides that are environmentally friendly for the management of fruit flies. The use of microbial control agents such as fungi in pest suppression is considered suitable since micro-organisms usually exert low environmental impact and are target specific. Application methods of bio-pesticides in the environment, which use minimal amount of inoculum is currently under improvement from inundative to auto-inoculative methods. Such devices are usually designed to attract insects into focus of the entomopathogen and use the insect as a vector for transmission of pathogen to other members of its population. …

Aku-Nama : Winter 2010, Volume 3, Issue 2, Aga Khan University Alumni Association

The Aga Khan University Newsletter and Magazine for Alumni (AKU-NAMA)

No abstract provided.

Shared Understanding Or Consensus-Masked Disagreement? The Anti-Torture Framework In The Convention On The Rights Of Persons With Disabilities, Janet E. Lord

Articles

No abstract provided.

Immunotherapy For Sepsis - A New Approach Against An Ancient Foe, Richard S. Hotchkiss, Steven Opal

Open Access Publications

No abstract provided.

Blood Meal Analysis To Identify Reservoir Hosts For Amblyomma Americanum Ticks, Brian F. Allan, Lisa S. Goessling, Gregory A. Storch, Robert E. Thach

Open Access Publications

No abstract provided.

Characterization Of Streptolysin O As The Translocator For The Cytolysin-Mediated Translocation Process In Streptococcus Pyogenes, N'Goundo Magassa

All Theses and Dissertations (ETDs)

Secretion of toxins into host cells is an important component of microbial pathogenesis. In order to gain access to the host cell cytosol, toxins must cross the plasma membrane, traverse the membrane of endocytic vesicles, or cross the membrane of organelles. The process cytolysin-mediated translocation: CMT) in Streptococcus pyogenes uses the pore-forming protein streptolysin O: SLO) to translocate the S. pyogenes NAD+-glycohydrolase: SPN) effector into the cytosol of eukaryotic host cells. Although, the fundamentals of this protein translocation process are established the details of the mechanism remain elusive. The current studies illustrate that the translocation process is more complex than …

Poxvirus Complement Control Proteins Are Expressed On The Cell Surface Through An Intermolecular Disulfide Bridge With The Viral A56 Protein, Brian Dehaven

Biology Faculty Scholarship

The vaccinia virus (VACV) complement control protein (VCP) is an immunomodulatory protein that is both secreted from and expressed on the surface of infected cells. Surface expression of VCP occurs though an interaction with the viral transmembrane protein A56 and is dependent on a free N-terminal cysteine of VCP. Although A56 and VCP have been shown to interact in infected cells, the mechanism remains unclear. To investigate if A56 is sufficient for surface expression, we transiently expressed VCP and A56 in eukaryotic cell lines and found that they interact on the cell surface in the absence of other viral proteins. …

The Vaccinia Virus Complement Control Protein Modulates Adaptive Immune Responses During Infection, N. M. Girgis, Brian Dehaven

Biology Faculty Scholarship

Complement activation is an important component of the innate immune response against viral infection and also shapes adaptive immune responses. Despite compelling evidence that complement activation enhances T cell and antibody (Ab) responses during viral infection, it is unknown whether inhibition of complement by pathogens alters these responses. Vaccinia virus (VACV) modulates complement activation by encoding a complement regulatory protein called the vaccinia virus complement control protein (VCP). Although VCP has been described as a virulence factor, the mechanisms by which VCP enhances VACV pathogenesis have not been fully defined. Since complement is necessary for optimal adaptive immune responses to …

Bicarbonate Enhances Expression Of The Endocarditis And Biofilm Associated Pilus Locus, Ebpr-Ebpabc, In Enterococcus Faecalis, Agathe Bourgogne, L Charlene Thomson, Barbara E Murray

Faculty, Staff and Student Publications

BACKGROUND: We previously identified ebpR, encoding a potential member of the AtxA/Mga transcriptional regulator family, and showed that it is important for transcriptional activation of the Enterococcus faecalis endocarditis and biofilm associated pilus operon, ebpABC. Although ebpR is not absolutely essential for ebpABC expression (100-fold reduction), its deletion led to phenotypes similar to those of an ebpABC mutant such as absence of pili at the cell surface and, consequently, reduced biofilm formation. A non-piliated ebpABC mutant has been shown to be attenuated in a rat model of endocarditis and in a murine urinary tract infection model, indicating an important participation …

Caring For The Diaspora: The Life And Work Of Nora Chengeto Tapiwa Of Zimbabwe, Sofia Javed

Kroc IPJ Research and Resources

In the following pages, you will find narrative stories about a Woman PeaceMaker, along with additional information to provide a deep understanding of a contemporary conflict and one person’s journey within it. These complementary components include a brief biography of the peacemaker, a historical summary of the conflict, a timeline integrating political developments in the country with personal history of the peacemaker, a question-and-answer transcript of select interviews, and a table of best practices in peacebuilding as demonstrated and reflected on by the peacemaker during her time at the Joan B. Kroc Institute for Peace & Justice.

A dedicated activist, …

Physical And Chemical Interactions Between Marine Phytoplankton And Particle-Colonizing Bacteria, Jason Robert Graff

Open Access Dissertations

It is inherently difficult to study the direct interactions between marine microbes due to the size of the organisms and microscale environments in which they exist. Recent evidence suggests that the direct associations between marine phytoplankton and particle-colonizing bacteria can influence marine microbial community succession, microalgal primary production, toxin production by marine phytoplankton, and have potential implications for human health. The goal of this dissertation was to elucidate some of the physical and chemical mechanisms that regulate microbial interactions, particularly the colonization of phytoplankton and other marine particles by bacteria. To accomplish these goals, I used a creative and multi-disciplinary …

Phase Six Pandemic: A Call To Re-Evaluate Federal Quarantine Authority Before The Next Catastrophic Outbreak, John T. Clarkson

Georgia Law Review

At some point in the future, the United States will confront a deadly pandemic. One potential response to blunt the impact of an outbreak may be the use of a large-scale quarantine. The current statutory framework governing federal quarantine authority, however, is outdated and in need of revision. Any reform must acknowledge that the federal government may be best positioned to respond to national health threats and must simultaneously create limits on federal power to ensure a proper balance of power remains between the states and the federal government. This Note proposes that Congress focus its reform efforts on adding …

Theory Of Island Biogeography On A Microscopic Scale: Organic Aggregates As Islands For Aquatic Pathogens, M. M. Lyons, J. E. Ward, Holly Gaff, R. E. Hicks, J. M. Drake, Fred C. Dobbs

OES Faculty Publications

Four predictions of the MacArthur-Wilson theory of island biogeography were evaluated to assess the degree to which detrital-based organic aggregates (e.g. marine snow, organic detritus, and bioflocs) may provide a favorable microhabitat (i.e. an 'island') for bacteria in general, and specifically aquatic pathogens. We demonstrate the theory's relevance for microbial communities in aquatic environments by describing the community metabolic response and functional diversity of individual organic aggregates while documenting the persistence of potential pathogens and fecal indicator bacteria. Our results support the 4 predictions, including a significant species-area relationship, consistency of species richness at equilibrium, non-zero level of species turnover …

Effects Of Livestock Antibiotics On Nitrification, Denitrification, And Microbial Community Compositon In Soils Along A Topographic Gradient, Sagarika Banerjee

University of Kentucky Master's Theses

Several types of antibiotics (roxarsone, virginiamycin, and bacitracin) are widely included in poultry feed to improve animal growth yields. Most of the antibiotics are excreted in manure which is subsequently applied to soils. One concern with this practice is that antibiotics may affect several microbially-mediated nutrient cycling reactions in soils that influence crop productivity and water quality. The main objectives of this study were to determine the effects of livestock antibiotics on nitrification, denitrification, and microbial community composition in soils along a topographic gradient. These objectives were addressed in a series of lab experiments by monitoring changes in inorganic N …

Progress Towards The Synthesis Of Type B Polycyclic Polyprenylated Acylphloroglucinol 7-Epi-Clusianone, Pushpa Suresh Jayasekara Mudiyanselage

University of Kentucky Doctoral Dissertations

Plants of the family Guttiferae produce polycyclic polyprenylated acylphloroglucinols (PPAPs), which have interesting biological activities including anticancer and antibacterial properties. The main structural features of PPAPs comprise of bicyclo[3.3.1]nonane-2,4,9-triketone with one acyl group together with prenyl, geranyl, or other C₁₀H₁₇ groups. 7-epi-Clusianone, a type B PPAP with C-7 endo stereochemistry, is being approached by establishing the cis relationship with C(4) allyl group and C(2) methyl ester in the early stage of the synthesis. Then C(2) methyl ester is converted to alkyne aldehyde and syn reduction followed by intramolecular aldol reaction to give bicyclo[3.3.1]nonane structure with …

Improvement Of New Oil Crops For Kentucky, Watchareewan Jamboonsri

University of Kentucky Doctoral Dissertations

Three oil crops, chia (Salvia hispanica L.), flax (Linum usitatissimum L.), and castor (Ricinus communis L.), were studied because of their nutritional and industrial values. Chia and flax are rich in an ω3 fatty acid, α-linolenic acid, and castor is a very high oil producer and high in a hydroxy fatty acid. Ethyl methanesulfonate (EMS) and gamma rays were employed to mutagenize chia seeds to produce early flowering mutants. The M₁ population was grown and induced to flower by short-day photoperiods. The M₂ population was planted in the field in Lexington, KY in 2008. Early …

Synthesis Of Some Pyridazine Derivatives Carrying Urea, Thiourea, And Sulfonamide Moieties And Their Antimicrobial Activity, Deni̇z S. Doğruer, Şölen Urlu, Ti̇jen Önkol, Berri̇n Özçeli̇k, M. Fethi̇ Şahi̇n

Turkish Journal of Chemistry

Some pyridazine derivatives carrying urea, thiourea, and sulfonamide groups were synthesized and evaluated for their antimicrobial activity against gram-positive and gram-negative bacteria, and fungi by using broth microdilution. The structures of these new compounds were confirmed by ^1H-NMR, mass spectrum, and elemental analysis. The synthesized compounds exhibited generally promising inhibitory activity against S. aureus (MIC ranging from 2 to 4 \mu g/mL) and E. coli (MIC ranging from 4 to 16 \mu g/mL). Moreover, all compounds showed antifungal activity against both C. albicans and C. parapsilosis, with a MIC value of 8 \mu g/mL.

Honors @ Georgia Southern, University Honors Program Students And Staff, Georgia Southern University

Honors College: News & Publications (2008-2020)

• A New Home for the Program
• Undergraduate Research
• Meet the new Assistant Director Dr. Francis Desiderio
• Honors in Action
• Alumni Notes

Managing Female Foreign Domestic Workers In Singapore: Economic Pragmatism, Coercive Legal Regulation, Or Human Rights?, Tan K. B. Eugene

Research Collection Yong Pung How School Of Law

Singapore's immigration discourse is deeply influenced by its need to “right-size” its population. As a society that has and remains in need of immigration, contemporary immigration and globalization have rigorously challenged the conventional thinking and understanding of citizenship, as well as notions of who belongs and who does not. Nevertheless, international marriages and pervasive in-and out-migration for purposes of employment, study, and family, conspire to make more pronounced the decoupling of citizenship and residence in Singapore. This transnational dimension sits uncomfortably with the policy makers' desire for, and the imperatives of, state sovereignty, control, and jurisdiction.Although one quarter of people …

Synthesis And Antimicrobial Activities Of 2-(5-Mercapto)-1,3-Oxadiazol-2-Ylmethyl-1,2,4-Triazol-3-One Derivatives, Nesli̇han Demi̇rbaş, Ahmet Demi̇rbaş, Hakan Bektaş, Şengül Alpay Karaoğlu, Deni̇z Şahi̇n

Turkish Journal of Chemistry

The synthesis of 4-amino-2-[(5-mercapto-1,3,4-oxadiazol-2- yl)methyl]-5-(4-methylphenyl)-2,4-dihydro-3H-1,2,4-triazol-3-one (2) and 4-amino-2-[(4-amino-5-mercapto-4H-1,2,4-triazol-3-yl)methyl]- 5-(4-methylphenyl)-2,4-dihydro-3H-1,2,4-triazol-3-one (5) was performed starting from 2-[4-amino-3-(4-methylphenyl)-5-oxo-4,5- dihydro-1H-1,2,4-triazol-1-yl]acetohydrazide (1). The treatment of 2 and 5 with 4-fluorobenzaldehyde (for 8) or salicylaldehyde (for 6) afforded the corresponding Schiff bases (6 and 8). The condensation of 5 with phenacyl bromide produced 4-amino-5-(4-methylphenyl)-2- [(6-phenyl-7H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazin-3-yl)methyl]- 2,4-dihydro-3H-1,2,4-triazol-3-one (7). The alkylation of 2 and 8 was carried out by using methyl iodide in basic media. Compounds 3 and 10a,b were prepared by aminoalkylation of 2 and 8 with 4-fluorophenyl piperazine, morpholine, or 4-methyl piperazine in the presence of formaldehyde. The synthesized compounds were screened for their antimicrobial activities …

Toward A Grounded Theory On The Management Of Orphanages In South Africa And Zimbabwe, Anna Siyavora

Walden Dissertations and Doctoral Studies

The number of orphaned children in many parts of Africa is increasing as their parents die from human immunodeficiency virus (HIV) or acquired immune deficiency syndrome (AIDS). The research problem addressed in this qualitative study was lack of understanding by others about how the managers of orphanages in 2 African countries -- South Africa and Zimbabwe - were responding to the emotional and social needs of these orphans. The purpose of this study was to develop an orphanage management theory or model that could replicate the African kinship environment in the orphanages under study. Nurturing leadership theory provided the conceptual …