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Multiscale Genomic Analysis Of The Corticolimbic System: Uncovering The Molecular And Anatomic Substrates Of Anxiety-Related Behavior, Khyobeni Mozhui

Theses and Dissertations (ETD)

Genetic diversity generates variation at multiple phenotypic levels, ranging from the most basic molecular to higher-order cognitive and behavioral traits. The far-reaching impact that genes have on higher traits is apparent in several neuropsychiatric conditions such as stress and anxiety disorders. Like most, if not all, neural phenotypes, stress, anxiety, and other emotion-related traits are extremely complex and are defined by the interplay of multiple genetic, environmental, experiential, and epigenetic factors.

The work presented in this dissertation is a multi-scalar, integrative analysis of the molecular and neuroanatomic substrates that underlie emotion-related behavior. The amygdala is a principle component of the …

Pax5 Haploinsufficiency Cooperates With Bcr-Abl1 To Induce Acute Lymphoblastic Leukemia, Christopher B. Miller

Theses and Dissertations (ETD)

Acute lymphoblastic leukemia (ALL) is the commonest pediatric malignancy and comprises several distinct subtypes each with its own unique pathogenesis, clinical behavior, and response to therapy. Chromosomal aberrations are a hallmark of ALL but alone fail to induce leukemia. Pediatric ALLs can be divided into several categories based on the expression of several genetically conserved chromosomal translocations including the t(9,22)[BCR-ABL1], t(1,19)[TCF3-PBX1], t(12,21)[ETV6-RUNX1], MLLrearranged leukemia’s, hyperdiploid and hypodiploid karyotypes, and T-lineage leukemia. Each translocation confers a characteristic transforming phenotype within the cell in which it originates but is alone insufficient to induce overt leukemia. …

Diffusion Tensor Imaging In Pediatric Brain Tumor Patients, Nicholas S. Phillips

Theses and Dissertations (ETD)

In this dissertation, we outline our efforts to introduce an advanced MRI imaging technique called Diffusion Tensor Imaging (DTI) to the pediatric brain tumor population. We discuss the theory and application of DTI as it was performed in a series of translational investigations at St. Jude Children’s Research Hospital. We present evidence of how the introduction of this technique impacted diagnosis, and treatment. And finally, we demonstrate how DTI was used to investigate cognitive morbidities associated with cancer treatment and how this research provided insight into the underlying pathophysiology involved in the development of these treatment sequela.

This research has …

Cost Burden Of The ‘Presenteeism’ Health Outcome In A Diverse Nurse And Pharmacist Workforce: Practice Models And Health Policy Implications, Carol L. Warren

Theses and Dissertations (ETD)

The complex phenomenon of presenteeism is an undesirable health outcome that occurs when employees remain present on-the-job with lowered work productivity caused by personal health conditions. The cost burden of presenteeism in healthcare professionals has been under-explored and the cost burden of presenteeism across racial and ethnic minority employees has been un-explored. Aims of this research were to describe presenteeism and its cost burden among nurses and pharmacists and to determine distinctness of differences across racial/ethnic groups within these professions. In exploring presenteeism, the focus was on recognizing it, characterizing it, and measuring it. In monetizing presenteeism, its costs burden …

The Role Of Inflammation In The Early Radiation Response, Christy Marie Wilson

Theses and Dissertations (ETD)

Radiation is one of the principal treatments for adults and children with brain tumors, and is one of the oldest established treatments for tumors of all types. Currently, the limiting factor for the use of radiation is the effect on normal tissue adjacent to the tumor. Toxicity, including early and late effects from radiation, limits the dose administered to the tumor and reduces the probability of cure. This work has three aims in its attempt to understand and limit early radiation damage: characterize the role of the inflammatory molecules tumor necrosis factor alpha (TNF) and intracellular adhesion molecule-1 (ICAM-1) in …

A Pharmacokinetics And Pharmacodynamics (Pk/Pd) Guided Approach To Lead Optimization Of Nitrofuranylamide Anti-Tuberculosis Agents, Nageshwar Rao Budha

Theses and Dissertations (ETD)

Currently used treatment strategies for tuberculosis (TB) involve administration of multiple drug combinations for a minimum of 6-9 months. However, these prolonged regimens do not always achieve sterilization, as evidenced by post-therapy relapse in a subgroup of treated individuals. In an effort to develop novel therapeutic agents for TB a new class of chemical agents, nitrofuranylamides, is being developed at the University of Tennessee Health Science Center. We hypothesized that the application of an iterative pharmacokinetics and pharmacodynamics (PK/PD) guided approach would facilitate the optimization of nitrofuranylamide lead compounds suitable for further development.

First, we examined the biopharmaceutic properties and …

Insights Into Btb-Cul3 Ubiquitin Ligases From The Structures Of Spop-Substrate Complexes, Min Zhuang

Theses and Dissertations (ETD)

Cullin-Ring ubiquitin ligases (CRLs) are E3 complexes that specifically recognize substrates through substrate adaptors. In the largest CRL subfamily, Cul3 binds a BTB domain, and a protein-interaction domain such as MATH recruits substrates for ubiquitination. Here we present biochemical and structural analyses of the MATH and BTB domain containing protein, SPOP, which regulates diverse signaling pathways. First, we identified a conserved SPOP Binding Consensus (SBC) motif in the transcriptional regulator Ci, the protein phosphatase Puc, and the chromatin component MacroH2A. The SBC motif specifically binds the MATH domain of SPOP, and is required for Puc ubiquitination in vitro and in …

Structural And Kinetics Studies Of The Enzyme Dihydropteroate Synthase And The Implications For Antibiotic Resistance, Katherine A. Ayers

Theses and Dissertations (ETD)

The goal of this study is to develop small molecule inhibitors of DHPS for the treatment of a variety of infectious diseases. According to the World Health Organization (WHO), infectious diseases kill more than 13 million people worldwide every year making it the second leading cause of death behind cardiovascular disease. The sulfonamide class of drugs has been in use since the 1930’s to treat many infectious agents and act by targeting the enzyme dihydropteroate synthase (DHPS) of the prokaryotic and lower eukaryotic folate pathway.

DHPS is an ideal drug target because humans do not synthesize folate de novo and …

Effect Of Obesity On The Traditional And Emerging Cardiovascular Disease Risk Factors In African American Women, Queen Obiageli Henry-Okafor

Theses and Dissertations (ETD)

Background: Obesity is a growing health care concern with cardiovascular disease (CVD) implications. African American women (AAW) have the highest prevalence rate of obesity and highest CVD morbidity and mortality rate of all ethnic groups. The traditional CVD risk factors have not been sufficient to explain this disparity in disease prevalence and outcomes. Current knowledge is limited regarding the interaction between various levels of adiposity and both traditional and emerging CVD risk factors, particularly in AAW. This study sought to explore these interactions.

Methods: The study design was a cross-sectional, descriptive, correlational analysis of 48 AAW ages 18 to 45 …

Evolutionary Analysis Of Host Proteins Cd4, Cxcr4 And Ccr5, And Hiv/Siv Gp12, Lana Bunning

Biological Sciences

The acquired immune deficiency syndrome, AIDS, is a growing epidemic in the United States and the world. Since its discovery in 1981, the virus that causes AIDS, human immunodeficiency virus (HIV), has escalated. .Certain African ape (i.e., chimpanzees and gorillas) and monkey species are known to harbor forms of the virus termed SIV (simian immunodeficiency virus). Chimpanzees are the natural hosts of the SIV strains from which HIV-I evolved, but do not rapidly progress to AIDS, unlike their human relatives. In the wild, gorillas have been observed to harbor SIV, but this species' disease progression is currently unknown. As the …

Immediate Effect Of Complete Denture Occlusal Errors On Masticatory Muscle Emg Activity In Denture Wearers: A Pilot Study, Swati Ahuja

Theses and Dissertations (ETD)

In the last three decades, little scientific progress has been seen in relation to the occlusal aspects in complete dentures. Equal distribution of loading forces to the edentulous denture foundation is an important factor affecting stability, patient comfort and acceptance of complete dentures. Also, understanding the functional behavior of masticatory muscles for complete denture wearers is important for diagnosing and planning the clinical treatment.

The purpose of the present study was to determine the effect of unilateral and bilateral occlusal interferences on the masticatory muscle activity in edentulous patients, and how patients respond to occlusal interferences both physiologically and psychologically …

Regulation Of Pyruvate Dehydrogenase Kinase 4 By Thyroid Hormone / Role Of Peroxisome Proliferator Activated Receptor Gamma Coactivator-1 Alpha And Ccaat Enhancer Binding Protein, Ramy Naguib Attia

Theses and Dissertations (ETD)

Pyruvate dehydrogenase kinase 4 (PDK4) regulates pyruvate oxidation through the phosphorylation and inhibition of the pyruvate dehydrogenase complex (PDC). The PDC catalyzes the conversion of pyruvate to acetyl-CoA and it is an important control point in glucose and pyruvate metabolism. Previous studies had reported that PDK4 gene expression is induced by thyroid hormone (T3). These studies did not investigate the mechanisms by which T3 regulated PDK4 gene expression. I have examined the role of the thyroid hormone receptor (TR), transcriptional coactivators especially the peroxisome proliferator activated receptor gamma coactivator-1 (PGC-1α) and other transcription factors that act as accessory factors in …

Analysis Of Selective Petrochemicals: A Case Study Of The Houston Texas Metropolitan Area, Joana Idakwo

Theses (Pre-2016)

With an increasing focus on environmental issues, a concentration on petrochemicals and their importance in commerce along with the role they play towards the issues affecting the ecosystem is a dominant topic of debate. One major product of crude oil is petroleum, which continues to play an important role in the social, economic, and political history of the United States and the world. Petroleum is a very complex mixture of many hydrocarbons. There are varieties of petroleum products, each of which has its own unique mix of molecules, which delineate its physical and chemical properties. Houston happens to be one …

Analysis Of Hazardous Material Incidents In The Gulf Coast Region: A Case Study Of Houston Texas., Latissha Clark

Theses (Pre-2016)

Petrochemical materials goods used in the manufacturing and production of many consumer products are moved on the United States' transportation network daily. These goods are included under the category of hazardous materials (HAZMAT). An estimated 800,000 shipments of HAZMAT occur every day. Data analysis and simulations can indicate patterns of incidents by using several different variables in the transport of these chemicals. Identifying the frequency enhances the pattern recognition to pinpoint potential dangerous situations. These situations could cause potential harm to the nation's critical infrastructure. Annual data are analyzed from the Pipeline and Hazardous Material Safety Administration (PHMSA), an agency …

Family History Of Alcohol Abuse Moderates Effectiveness Of A Group Motivational Enhancement Intervention In College Women, Joseph W. Labrie, Nashla Feres, Shannon R. Kenney, Andrew Lac

Heads Up!

This study examined whether a self-reported family history of alcohol abuse (FH+) moderated the effects of a female-specific group motivational enhancement intervention with first-year college women. First-year college women (N= 287) completed an initial questionnaire and attended an intervention (n=161) or control (n=126) group session, of which 118 reported FH+. Repeated measures ANCOVA models were estimated to investigate whether the effectiveness of the intervention varied as a function of one’s reported family history of alcohol abuse. Results revealed that family history of alcohol abuse moderated intervention efficacy. Although the intervention was effective in producing …

Methyl Septanoside Hydrolysis As A Measure Of Carbohydrate Ring Flexibility: Substrate Synthesis And Kinetics Analysis, Shawn Michael Miller

Honors Scholar Theses

A number of seven-membered ring systems have been found to have promising enzyme inhibiting properties. The common explanation for their efficacy is the apparent improved flexibility that the ring-system has over smaller rings. In an attempt to probe the validity of this hypothesis, unnatural seven-membered ring carbohydrates have been generated and subjected to kinetics analysis using hydrolysis. In particular, methyl α- and β-D-glycero-D-guloseptanoside were subjected to acid-catalyzed hydrolysis and monitored via HNMR experiments. The rate constants of the reactions were determined and compared to the hydrolysis rate constants of comparable pyranose carbohydrates and found to be significantly faster, indicating an …

Effect Of Work Intensification And Work Extensification On Women’S Health In The Globalised Labour Market, Jinky Leilanie Lu

Journal of International Women's Studies

This study examined the association between labour intensification due to work intensification and work extensification and ill health in women in certain manufacturing work in the Philippines. Work intensification is defined as more workload for each worker, and work extensification as less deadtime or work rest and more overtime. The sample was 23 establishments and 630 respondents. Workplace environment monitoring showed exposure to hazards such as noise, chemicals, poor ventilation, and poor illumination. The most prevalent illnesses and health problems were headache and coughs and colds. Results of focus group discussions showed adverse work conditions, hazard exposures among women workers, …

Understanding Polycyclic Aromatic Hydrocarbon Degradation By Soil Microbial Communites: The Concentration And Distribution Of Aromatic Dioxygenase Genes, Inigo Walker Howlett

Masters Theses and Doctoral Dissertations

The ability to individually identify and enumerate bacterial genes in the environment is an important development in the field of microbial ecology. Researchers are now able to quantify and map populations and communities, the presence of which could only be inferred until recently. Knowledge of this ecology is critical to understanding microbial responses to environmental pollutants, thereby contributing information useful in devising and monitoring bioremediation strategies. The following thesis therefore compares two aromatic dioxygenase genes, nidA and nahAc, which confer the ability for the bacteria carrying the genes to metabolize polycyclic aromatic hydrocarbons (PAHs), persistent pollutant byproducts of coal coking. …

Local Effects Of Culverts On Habitat Features And Fish Assemblages In Blue Ridge Streams, Daniel Huser

Masters Theses and Doctoral Dissertations

I studied environmental conditions, fish assemblages, and culvert features in 11 headwater streams (Tennessee and Conasauga river drainages) in Cherokee National Forest (Blue Ridge ecoregion), Tennessee, May-August 2008. Culvert-related effects on instream habitat and fish assemblages were measured at 10 stream sites: five sites contained culverts with artificial bottom substrates and five sites contained culverts with natural bottom substrates. On each stream, paired sampling reaches (reach length ~35 times mean reach width, drainage area <11 km 2) were established 50 m upstream and 50 m downstream of the culvert. Reaches downstream of culverts with artificial substrates (compared to upstream reaches; related-samples t test) exhibited greater water depths, lower gravel/sediment depths, and higher percentages of bedrock and boulder substrates (P < 0.10). Reaches downstream of natural substrate culverts (compared to upstream reaches) exhibited faster current velocities (P < 0.10). Fish abundance (predominantly western blacknose dace Rhinichthys obtusus) was consistently higher downstream than upstream (P < 0.10), regardless of the culvert type. In addition, reaches downstream of artificial substrate culverts exhibited reduced species evenness compared to upstream reaches. Mark-recapture experiments on two streams documented fish movements through a natural substrate culvert (similar to those in reference areas); however, movements through a perched pipe culvert were not detected. Data collected i n the present study suggest that culverts had localized effects on instream habitat and fish assemblages, and that certain types of culverts may impede fish dispersal.

Evaluating Web-Based Realistic Job Preview Formats: Applicant Attraction, Intentions To Pursue, And Job Acceptance Decisions, Laure L. Rodenbaugh

Masters Theses and Doctoral Dissertations

A recent trend in organizational selection is the use of web-based job search engines to post current employment opportunities. This study utilized a sample of junior and senior level undergraduate business and psychology students expected to actively be searching for permanent employment within one year. Three different job advertisement/RJP conditions were employed: presentation of standard/general job and company information, a written RJP with general company information, and a video-formatted RJP with general information about the company. Each condition was formatted to resemble those job postings currently in use on the web. Corresponding scales intended to measure the participant’s attraction to …

Predicting Athletic Performance: The Effects Of Judgment And Motivation, Matthew E. Smallwood

Masters Theses and Doctoral Dissertations

The purpose of this study was to examine the influence of athlete motivation and judgment on the relationship between athletic ability and athletic performance. Much existing empirical literature has focused on motivation and cognitive ability in relation to athletic performance, but athlete judgment has received relatively little research attention. It was hypothesized that high performers will have stronger judgment and motivation scores than will lower performers. Results suggest the ability to make strategic decisions as operationalized by Systemic Judgment may be predictive of Athletic Performance.

Effects Of Urbanization On Instream Habitat And Fish Assemblages In The Chattanooga Metropolitan Area, Tennessee-Georgia, Joshua Smith

Masters Theses and Doctoral Dissertations

Twenty-one stream sites in the Ridge and Valley ecoregion of Tennessee and Georgia were studied in 2008 to evaluate the effects of watershed urbanization on instream environmental parameters and fish assemblages. I also investigated changes in urbanization and stream conditions at 10 sites over a 10-year period (1998-2008). Electrofishing yielded 12,329 fish, composed of 38 species from eight families. Urbanization in the study watersheds was measured (using ArcGIS 9.3; released June, 2008) by calculating the building density and percent urban land use. Correlation analysis revealed that more urbanized watersheds were characterized by increased proportions of fine sediments and pool areas, …

The Role Of Emotional Intelligence In Leadership Effectiveness: A Meta-Analysis, Ashleigh D. Farrar

Masters Theses and Doctoral Dissertations

Leaders are an essential element of the business world. While good leaders can provide many benefits for an organization, unsuccessful leaders can be detrimental. The notion that emotional intelligence plays a part in whether a leader is effective or not effective has recently been introduced. This study sought to unify the literature evaluating the possible link between emotional intelligence and leadership effectiveness. Meta- analytic techniques were used to analyze this relationship. Results revealed that overall, there is a positive relationship between emotional intelligence and leadership effectiveness. Also, while the type of emotional intelligence measure used served as a moderator to …

Functional Characterization And Assembly Studies Of Carboxysomes In Halothiobacillus Neapolitanus, Zhicheng Dou

Dissertations

Functional characterization and assembly studies of carboxysomes in Halothiobacillus neapolitanus were pursued in order to understand the roles of carboxysomes in the carbon metabolism in H. neapolitanus and the assembly of carboxysomes in vitro and in vivo. Previously, a low abundance H. neapolitanus carboxysomal protein, CsoSCA was identified as a novel carboxysomal shell-bound carbonic anhydrase. The enzyme is thought to dehydrate the cytosolic bicarbonate to CO2, the substrate of the RuBisCO packaged within the carboxysome [14, 21]. In this study, the carboxysomal shell was identified as a diffusion barrier for CO2 and O2 molecules. The shell-bound CsoSCA protein facilitates the …

Oceanic-Atmospheric Modes Of Variability And Their Effect On River Flow And Blue Crab (Callinectes Sapidus) Abundance In The Northcentral Gulf Of Mexico, Guillermo Humberto Sanchez-Rubio

Dissertations

Oceanic-atmospheric modes of variability occur on interdecadal, multidecadal, decadal, and interannual timescales and their influence on climate around the world has been confirmed. The present study investigates Mississippi River and Pascagoula River flows in response to the influence of one or more of the four oceanic-atmospheric modes of variability: the Pacific Decadal Oscillation (PDO), the Atlantic Multidecadal Oscillation (AMO), the North Atlantic Oscillation (NAO), and the El Nino Southern Oscillation (ENSO). These modes of variability are present in phases: PDO warm (PDOw) and cold (PDOc), AMO warm (AMOw) and cold (AMOc), NAO positive (NAOp) and negative (NAOn), and ENSO warm …

Predator And Abiotic Effects On Hatching Phenotype And Survival Of Arboreal Frog Eggs With Implications For Phytoplankton, Jessica Hite

Theses and Dissertations

Historically studies have focused on either the terrestrial or aquatic environments independently. However, these systems are inherently linked through numerous pathways including organisms with complex life cycles. Both abiotic factors and predators of these organisms can influence connections by changing the number of prey moving across habitat boundaries and by changing the phenotype of prey. When the focal organisms are primary consumers, these effects may have important implications for ecosystem processes. My study investigated how terrestrial predators and abiotic factors affect the number and phenotype of herbivorous tadpole inputs into a tropical forest pond. I found that predators and abiotic …

De-Masking The Silence - Drama Therapy: For Children With Asd, Joshua M. Galligan

Theses and Dissertations

This dynamic form of pedagogy attempts to cross the interdisciplinary boundaries between theatre, psychology, and modern medicine. What I attempt to present in this thesis is a systematic developmental framework for the theoretical use of drama therapy with children suffering from Autism Spectrum Disorder. This approach has been based on in-depth interdisciplinary study which makes use of the most current research looking at the neurological makeup of the autistic brain. This systematic approach examines the mirror neuron system which has been labeled as a major defect in children with ASD. Through the use of theatrical techniques such as acting, movement, …

The I-70 Greenfield Rest Area Wetland Projects, Shih-Chieh Kao, Ting Pong Chang, Rebeka Sultana, Thomas Konopka, Rao S. Govindaraju, Barry Partridge

JTRP Technical Reports

On-site treatment of wastewater at highway rest areas poses some unique and difficult challenges because of the rural locale, high variability in wastewater flow rate and strength, and lack of knowledgeable personnel on-site. As a potential alternative, a constructed subsurface wetland system was built at the I-70 rest stop nearby Greenfield, Indiana, in 2003. This wetland system, mainly composed of three wetland cells, also includes draw-and-fill and recirculation mechanisms to increase oxygen transfer to the wastewater and improve the overall treatment performance. Special considerations for highway rest areas have been emphasized. A dynamic hydraulic model was developed to help characterize …

No Future For The Antarctic Treaty?, Sven Thatje, Richard B. Aronson

Ocean Engineering and Marine Sciences Faculty Publications

Climate change is exposing Antarctica’s natural resources to potentially irreversible degradation. The Antarctic Treaty, which turns 50 this year, has shielded the region’s sensitive terrestrial and marine ecosystems, but we are on the brink of the modern world’s last great land-grab. Several nations are making new claims in Antarctica by exploiting loopholes in the Treaty. The international community must now choose between letting the Treaty crumble in the face of these challenges or mobilizing to protect Antarctica’s largely pristine ecosystems on a permanent basis.

Targeted Delivery Of Surface Modified Nanoparticles: Modulation Of Inflammation For Acute Lung Injury, Hari R. Desu

Theses and Dissertations (ETD)

The objective of the study is to demonstrate alleviation of pulmonary inflammation associated with acute lung injury (ALI) using novel surface modified nanoparticles. ALI is characterized by three main pathological events: I) pulmonary edema, II) excessive pro-inflammatory cytokines and chemokines production from alveolar epithelial and endothelial cells and III) leukocyte migration from blood circulation into alveoli. Currently, there is no FDA approved pharmacological treatment available except the supportive mechanical ventilation therapy. Numerous clinical trials involving pharmacological therapies aimed at different pathological targets turned unsuccessful. National Institute of Heart Lung and Blood Institute (NHLBI) cited underappreciation of drug delivery systems as …