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Synchrotron X-Ray Visualisation Of Ice Formation In Insects During Lethal And Non-Lethal Freezing, Brent J. Sinclair, Allen G. Gibbs, Wah Keat Lee, Arun Rajamohan, Stephen P. Roberts, John J. Socha

Biological Sciences Faculty Research & Creative Works

Although the Biochemical Correlates of Freeze Tolerance in Insects Are Becoming Well-Known, the Process of Ice Formation in Vivo is Subject to Speculation. We Used Synchrotron X-Rays to Directly Visualize Real-Time Ice Formation at 3.3 Hz in Intact Insects. We Observed Freezing in Diapausing 3rd Instar Larvae of Chymomyza Amoena (Diptera: Drosophilidae), Which Survive Freezing If It Occurs above 214uC, and non-Diapausing 3rd Instar Larvae of C. Amoena and Drosophila Melanogaster (Diptera: Drosophilidae), Neither of Which Survive Freezing. Freezing Was Readily Observed in All Larvae, and on One Occasion the Gut Was Seen to Freeze Separately from the Haemocoel. There …

Cal Poly Mid-Year Commencement Set For Dec. 12, Public Affairs Office

Press Releases

No abstract provided.

Overexpression And Partial Purification Of Serine Hydroxymethyltransferase, Kimberly Rose Stevens

Chemistry and Biochemistry

No abstract provided.

Relationship Of Copper, Zinc And Selenium Status With Udder Health And Mastitis Incidence In The Cal Poly Holstein Herd, Michelle Van Ryn

Dairy Science

No abstract provided.

Algae As An Economical Protein Source For Dairy Cattle Nutrition, Brian Azevedo

Dairy Science

Dairying continues to change with economic and environmental demands of today’s fast moving society. Feed costs continue to rise and now account for a large majority of expenses on dairies; especially protein, which has become expensive to purchase. I believe that Algae can serve as a possible solution to aid in alleviating this problem. Algae grow rapidly and consume undesirable carbon dioxide to release oxygen to the environment, making it an environmentally friendly source of feed. There are particular strands of algae that are high in protein. During the course of this project, I intend to discover how effective protein …

2,5-Dichlorothiophene 1,1-Dioxide, Jonathan B. Briggs, Wenling Jia, Mikael D. Jazdzyk, Glen P. Miller

Chemistry

The complete molecule of the title compound, C(4)H(2)Cl(2)O(2)S, is generated by crystallographic twofold symmetry, with the S atom lying on the rotation axis. In the crystal, the molecules are linked by C-H center dot center dot center dot O hydrogen bonds..

Ethnomedicinal Studies Of Some Weeds In Crop Fields Of Marathwada Region, India, J. A. Dhole, N. A. Dhole, S. S. Bodke

Ethnobotanical Leaflets

The aim of the present work was the identification and documentation of ethnomedicinal weeds growing in crop fields in the Marathwada region of India (70o 5’-78o 5’ E longitude and 17o 5’-20o 5’ N longitude). Our survey was also aimed at the possibility of discovering new ways by which such plants could be better utilized for the welfare of human health. A total of 20 species of plants representing 18 Genera and 12 families were collected. In addition to the first hand collection of ethnomedicinal information from the inhabitants of the area, additional traditional uses were obtained through a study …

Ethnomedicinal Plants And Associated Traditional Knowledge Of Jogimatti Forest, Chitradurga District, Karnataka, India, V. T. Hiremath, T. C. Taranath

Ethnobotanical Leaflets

India has one of world’s richest medicinal plant heritages. The wealth is not only in terms of the number of unique species documented, but also in terms of the tremendous depth of traditional knowledge for the uses of human & livestock health and also for agriculture. The medicinal plant species are used by various ethnic communalities for human and veterinary health care, across the various ecosystems from Ladakh in the trans-Himalayas to the southern coastal tip of Kanyakumari and from the deserts of Rajasthan and Kachch to the hills of the Northeast. Chitradurga district at its extreme limits is situated …

Two Additional Invasive Scarabaeoid Beetles (Coleoptera: Scarabaeidae: Dynastinae) In Hawaii, Mary Liz Jameson, Darcy E. Oishi, Brett C. Ratcliffe, Grant T. Mcquate

University of Nebraska State Museum: Entomology Papers

Two species of dynastine scarab beetles are reported for the first time on the island of Hawaii: the Pasadena masked chafer, Cyclocephala pasadenae (Casey) (Scarabaeidae: Dynastinae: Cyclocephalini) from North America and Temnorhynchus retusus (Fabricius) (Scarabaeidae: Dynastinae: Pentodontini) from Africa. The Pasadena masked chafer is established on the island of Hawaii, is a recognized pest of turf grass, and is a concern for agriculture and native Hawaiian arthropod species. Temnorhynchus retusus is not known to be an agricultural pest, but its spread on the islands could pose a problem for Hawaiian native species. We provide an overview of each species, characteristics …

Distribution And Diversity Of Archaeal And Bacterial Ammonia Oxidizers In Salt Marsh Sediments, Nicole S. Moin, Katelyn A. Nelson, Alexander Bush, Anne E. Bernhard

Biology Faculty Publications

Diversity and abundance of ammonia-oxidizing Betaproteobacteria (β-AOB) and archaea (AOA) were investigated in a New England salt marsh at sites dominated by short or tall Spartina alterniflora (SAS and SAT sites, respectively) or Spartina patens (SP site). AOA amoA gene richness was higher than β-AOB amoA richness at SAT and SP, but AOA and β-AOB richness were similar at SAS. β-AOB amoA clone libraries were composed exclusively of Nitrosospira-like amoA genes. AOA amoA genes at SAT and SP were equally distributed between the water column/sediment and soil/sediment clades, while AOA amoA sequences at SAS were primarily affiliated with the …

The Classic, Winter 2009-2010, Public Relations

The Classic, 2000-2009

Zwemer View: A Mission to Live By; In Box; Eight Is Enough; Break for Missions; Crack Coders; Tough Decisions; Winning Hymn; Wrapped in Warmth; Of Course: Connected; Extreme Singing; Prof Appointments; Major Excellence; On the Road; To New Orleans With Love; Alumni Director Search; Vocation Connection; Face Value: Sara Sybesma Tolsma; Red Zone; First Dance; 1000 Words: Home Sweet Home; Free to Be: Students with disabilities thrive at Northwestern; Raider Romance; Honored: Four receive distinguished alumni awards: Darlene (De Beer '52) Vander Aarde, Jean (Tallman '75) and Drew Vogel '72, Randy Oostra '77; Red Weekend; Class Notes; New Arrivals; Marriages; …

Impact Of Parent’S Hiv Status On Their Uninfected Child—A Comparative Analysis Of The Child’S Healthcare Utilization, Access And Health Outcomes, Arijit Ganguli

Theses and Dissertations (ETD)

With the advent of effective antiretroviral medication and increased expectancy of life span among HIV-infected individuals has lead to an increase in the at-risk population of uninfected children living with their HIV-infected parent(s). The purpose of this study was to investigate the impact of parent’s HIV status on their child’s access to healthcare resources, healthcare utilization and health outcomes.

This was a cross-sectional study in which the information on the children of HIV seropositive parent(s) was collected through a face-to-face interview of the HIV-infected parents having children currently residing with them. The comparative group comprising of children of HIV seronegative …

Structural Characterization Of The C-Terminus Of The Cochlear Motor Protein Prestin: Intrinsic Disorder Linked To Voltage Sensor Function, Michael S. Podgorski

Theses and Dissertations (ETD)

The sensitivity of mammalian hearing relies upon the ability to amplify sound. Outer hair cells (OHCs) in the cochlea have motile properties that support this function. The motor protein called prestin resides in the lateral wall of the OHCs. Prestin undergoes voltage-dependent conformational changes, which correlates to the movement of charge through the membrane or non-linear capacitance (NLC). This property underlies OHC electromotility. A mechanistic understanding of prestin’s function remains unknown. Previous research has tied prestin’s C-terminus (residues 499-799) to the voltage-dependent events that occur in the membrane. The folding and biochemical properties of prestin’s C-terminus were characterized. This information …

Daily Announcements December 2009, Wofford College. Office Of Marketing And Communications

Communications Office Daily Announcements

No abstract provided.

Student Sample Perceptions Of Organic Food Products And Buying Habits, Alycia M. Deus

Agricultural Education and Communication

The purposes of this senior project were to identify college students’ perceptions and knowledge of organic food. This study also focuses on the respondents’ purchasing and budgeting of organic food. This information is essential to inform farmers and ranchers because it serves as an indicator of future market’s demands.

A thirteen item questionnaire was distributed to sixty-five students attending California Polytechnic State University, San Luis Obispo, enrolled in either Agricultural Education and Communication courses or Agricultural Business courses. Both upper and lower division classes were surveyed. The questionnaires were distributed and collected from June 2, 2009 to June 4, 2009. …

The Cresset (Vol. Lxxiii, No. 2, Advent/Christmas), Valparaiso University

The Cresset (archived issues)

No abstract provided.

Avian Advice, Winter 2009, Dale Bumpers College Of Agricultural, Food, And Life Sciences (University Of Arkansas, Fayetteville). Center Of Excellence For Poultry Science, University Of Arkansas (System). Cooperative Extension Service

Avian Advice

No abstract provided.

Tc17 Cd8 T Cells: Functional Plasticity And Subset Diversity, Adam J. Adler

UCHC Articles - Research

L-17-secreting CD8 T cells (Tc17) have been described in several settings, but little is known regarding their functional characteristics. While Tc1 cells produced IFN-γ and efficiently killed targets, Tc17 cells lacked lytic function in vitro. Interestingly, the small numbers of IFN-γ-positive or IL-17/IFN-γ-double-positive cells generated under Tc17 conditions also lacked lytic activity and expressed a similar pattern of cell surface proteins to IL-17-producing cells. As is the case for Th17 (CD4) cells, STAT3 is important for Tc17 polarization, both in vitro and in vivo. Adoptive transfer of highly purified, Ag-specific IL-17-secreting Tc17 cells into Ag-bearing hosts resulted in near complete …

Effect Of Hcv Rna Suppression During Peginterferon Alfa-2a Maintenance Therapy On Clinical Outcomes In The Halt-C Trial, Herbert L. Bonkovsky

UCHC Articles - Research

Background and Aims

The HALT-C trial demonstrated that low-dose peginterferon maintenance therapy was ineffective in preventing clinical outcomes in patients with chronic hepatitis C, advanced fibrosis and failure to achieve a sustained virologic response during lead-in phase treatment with standard dose peginterferon/ribavirin. This analysis was performed to determine if suppressing HCV RNA during the trial was associated with a reduction in clinical outcomes.

Methods

764 patients treated during the lead-in phase of HALT-C were randomized to either peginterferon alfa-2a (90 mcg/week) maintenance therapy or no treatment (control) for 3.5 years. Clinical outcomes included an increase in Child-Turcotte-Pugh score, ascites, spontaneous …

Lucerne Guidelines For Western Australia Principles For Integrating A Perennial Pasture Into Broadacre Dryland Farming Systems, Diana Fedorenko, Perry Dolling, Christopher Loo, Tom Bailey, Roy Latta

Bulletins 4000 -

Lucerne, often regarded as the queen of forages, has been used in agriculture for centuries. In Western Australia, however, its potential has never been fully realised despite its well-documented ability to dewater soils and reduce groundwater recharge. Over the years adoption has been limited by a range of factors including pests and diseases, soil acidity, grazing management and economics. During the last decade research has addressed these issues, paving the way for much greater impact at the farm and catchment level.

Primetimes Newsletter, Winter 2009, Office Of Lifespan Studies

PrimeTimes Newsletter

PrimeTimes is the newsletter of the Office of Lifespan Studies in the College of Science at Coastal Carolina University.

Research Supports Un Millennium Development Goals, Sarah Huggett

Research Trends

The UN Millennium Development Goals aim to combat the effects of extreme poverty. Bringing together governments, industry and research, this global effort hopes to solve some of our greatest challenges by 2015. Research Trends looks at how research on HIV/AIDS, tuberculosis and malaria measures up to the impact of these diseases.

Gender Differences In Output Quality And Quantity Under Competition And Time Constraints: Evidence From A Pilot Study, Olga Shurchkov

Fondazione Eni Enrico Mattei Working Papers

Gender gaps in income and level of position in the workplace are widespread. One explanation for this inequality is that the genders perform differently under competitive conditions, as previous experimental studies have found a significant gender gap in competitive tasks that are perceived to favor men. In this paper, we use a verbal task that is perceived to favor women and find no gender difference under competition per se. We also reject the hypothesis that a .stereotype threat. explains the inability of women to improve performance under competition. We propose an alternative explanation for gender inequality: namely, that women and …

Nuclear Versus Coal Plus Ccs: A Comparison Of Two Competitive Base-Load Climate Control Options, Massimo Tavoni, Bob Van Der Zwaan

Fondazione Eni Enrico Mattei Working Papers

In this paper we analyze the relative importance and mutual behavior of two competing base-load electricity generation options that each are capable of contributing significantly to the abatement of global CO2 emissions: nuclear energy and coal-based power production complemented with CO2 capture and storage (CCS). We also investigate how, in scenarios from an integrated assessment model that simulates the economics of a climate-constrained world, the prospects for nuclear energy would change if exogenous limitations on the spread of nuclear technology were relaxed. Using the climate change economics model WITCH we find that until 2050 the resulting growth rates of nuclear …

Charge Switch Nucleotides, John G. K. Williams, Gregory R. Bashford, Jiyan Chen, Dan Draney, Nara Narayanan, Bambi Reynolds, Pamela Sheaff

Biomedical Imaging and Biosignal Analysis Laboratory

The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charged-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation Without interference from unincorporated NPs and Without illuminating the polymerase-DNA complex.

Lmproving Education Through Brain Research, Lisa J. Ihnen

Master of Education Program Theses

Educational practices are improved because of brain research. Cognitive and behavior sciences have provided important concepts for educational practice. Within recent decades, neuroscience has also contributed to the field of brain research, thus enhancing our understanding oflearning. Three main concepts from neuroscience are explored: synaptogenesis, critical periods, and enriched environments. When the entire body of knowledge from all disciplines of brain research is viewed together, then fmdings can be applied to educational practices.

Vitisnet: ‘‘Omics’’ Integration Through Grapevine Molecular Networks, Jerome Grimplet, Grant R. Cramer, Julie A. Dickerson, Kathy Mathiason, John Van Hemert, Anne Y. Fennell

Agronomy, Horticulture and Plant Science Faculty Publications

Background: Genomic data release for the grapevine has increased exponentially in the last five years. The Vitis vinifera genome has been sequenced and Vitis EST, transcriptomic, proteomic, and metabolomic tools and data sets continue to be developed. The next critical challenge is to provide biological meaning to this tremendous amount of data by annotating genes and integrating them within their biological context. We have developed and validated a system of Grapevine Molecular Networks (VitisNet).

Methodology/Principal Findings: The sequences from the Vitis vinifera (cv. Pinot Noir PN40024) genome sequencing project and ESTs from the Vitis genus have been paired and the …

Synthesis And Cytotoxic Analysis Of Some Disodium 3Β,6Β-Dihydroxysterol Disulfates, Jianguo Cui, Hui Wang, Yi Xin, Aimin Zhou

Chemistry Faculty Publications

Disodium 3β,6β-dihydroxy-5α-cholestane disulfate (1) was synthesized in 4 steps with a high overall yield from cholesterol. First, cholesterol (4a) was converted to cholest-4-en-3,6-dione (5a) via oxidation with pyridinium chlorochromate (PCC) and then 5a was reduced by NaBH4 in the presence of NiCl2 to produce cholest-3β,6β-diol (6a). The reaction of 6a with the triethylamine-sulfur trioxide complex generated diammonium 3β,6β-dihydroxy-5α-cholestane disulfate (7a) and the treatment of 7a by cation exchange resin 732 (sodium form)(Na+) yielded the target steroid 1. Disodium 24-ethyl-3β,6β-dihydroxycholest-22-ene disulfate (2) and disodium 24-ethyl-3β,6β-dihydroxycholestane disulfate (3) were synthesized using a similar method. The cytotoxicity of these compounds against Sk-Hep-1 (human …

Phylogeography And Postglacial Dispersal Of Smallmouth Bass (Micropterus Dolomieu) Into The Great Lakes, W. Calvin Borden, Robert A. Krebs

Biological, Geological, and Environmental Faculty Publications

Refugia and dispersal routes of smallmouth bass (Micropterus dolomieu) into the Great Lakes were identified using 427 mitochondrial sequences from across their native range. Overall, smallmouth bass accessed the Great Lakes via long-distance dispersal from multiple refugia, and the distribution of genetic variation reflected the consequences of vicariant, dispersal, and paleogeological events. Proximity of lakes to glacial outlets had a greater predictive power on the resulting distribution of mitochondrial diversity relative to interbasin migration. Populations in the Eastern and Interior Highlands contained the most divergent and oldest haplotypes, reflecting their role as glacial refugia and subsequent high incidence of endemicity. …

Remodeling Of The Tight Junction During Recovery From Exposure To Hydrogen Peroxide In Kidney Epithelial Cells, Jeannette E. Gonzalez, R. J. Digeronimo, D'Ann E. Arthur, Jonathan M. King

Biology Faculty Research

Renal ischemia-reperfusion injury results in oxidative stress-induced alterations in barrier function. Activation of the mitogen-activated protein (MAP) kinase pathway during recovery from oxidative stress may be an effector of oxidant-induced tight junction reorganization. We hypothesized that tight junction composition and barrier function would be perturbed during recovery from oxidative stress. We developed a model of short-term H₂O₂ exposure followed by recovery using Madin Darby canine kidney (MDCK II) cells. H₂O₂ perturbs barrier function without a significant cytotoxic effect except in significant doses. ERK-1/2 and p38, both enzymes of the MAP kinase pathway, were activated …