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Transcriptional Reprogramming Of Gene Expression In Bovine Somatic Cell Chromatin Transfer Embryos, N. Rodriguez-Osorio, Zhongde Wang, G. P. Page, J. M. Robl, E. Memili

Animal, Dairy, and Veterinary Science Faculty Publications

Background Successful reprogramming of a somatic genome to produce a healthy clone by somatic cells nuclear transfer (SCNT) is a rare event and the mechanisms involved in this process are poorly defined. When serial or successive rounds of cloning are performed, blastocyst and full term development rates decline even further with the increasing rounds of cloning. Identifying the "cumulative errors" could reveal the epigenetic reprogramming blocks in animal cloning. Results Bovine clones from up to four generations of successive cloning were produced by chromatin transfer (CT). Using Affymetrix bovine microarrays we determined that the transcriptomes of blastocysts derived from the …

“And The Word Was Made Flesh”: A Latter-Day Saint Exegesis Of The Blood And Water Imagery In The Gospel Of John, Eric D. Huntsman

Studies in the Bible and Antiquity

Critical to understanding the widespread symbolism and imagery pointing to Jesus Christ in the New Testament is an exegetical grasp of the content--that is, an understanding of the historical, literary, and theological context of the language. The image of water recurs frequently throughout the New Testament Gospels as a symbol of the Savior's purity, cleansing power, true doctrine, and so forth. Similarly, blood is used often to reflect the sacred mission of Christ and the price of our salvation. This article investigates this imagery, particularly as used by Apostle John, to explain the significance of the Savior's mission in mortality …

Natal Habitat Use By Dragonflies Along Landscape Gradients In Rhode Island, Maria Adella Aliberti Lubertazzi

Open Access Dissertations

Urban environments often support depauperate insect faunas, although they can serve as suitable habitats for some taxa. The potential value of urban wetlands as habitat for regional dragonfly populations has not been well studied. Landscape patterns of natal habitat use by lentic dragonflies were studied at small wetlands in Rhode Island, U.S.A. during three field seasons (2004, 2005, 2006). Dragonfly exuviae were collected along defined perimeter paths on six (2004) or five (2005, 2006) site visits per season (May- October). The exuviae were identified in the laboratory, and the number collected per species/hour was tallied for each field season. Three …

Nitric Oxide Cytotoxicity And Functions Of Iron-Sulfur Enzymes In Escherichia Coli, Binbin Ren

LSU Doctoral Dissertations

Iron-sulfur proteins are ubiquitous in biological processes. Here we report that NO, a physiological free radical, can effectively inhibit cell growth of Escherichia coli in minimal medium under anaerobic growth conditions. Fractionation of the cell extracts obtained from NO-exposed cells shows a broad distribution of the protein-bound dinitrosyl-iron complexes (DNICs) formed by NO. On the other hand, the cell growth of E. coli can be restored when NO-exposed cells are either supplemented with the branched-chain amino acids (BCAAs) anaerobically or returned to aerobic growth conditions. It turns out that dihydroxyacid dehydratase (IlvD), an iron-sulfur enzyme essential for the BCAAs biosynthesis, …

The Feasibility Of Using Select Landscape Species For Phytoremediation Of Cumene And 4-Cumylphenol Contaminated Groundwater, Kathryn Karsh Fontenot

LSU Doctoral Dissertations

Chemical manufactures are often associated with a negative publicity due to chemical spills that can cause human health problems and environmental pollution. Innovative methods such as phytoremediation in lieu of traditional remediation methods are being researched to determine environmentally friendly options for remediation. Phytoremediation was studied as an alternative remediation method for removal of chemicals in a contaminated groundwater plume in Louisiana. The main constituents of concern in the plume were cumene and 4-cumylphenol. Two pilot phytotoxicity studies were funded to determine an optimum tree species for removal and control of the constituents of concern. A greenhouse and hydroponic system …

Detection Of Stress Biomarkers In Sperm, Embryonic, And Early Larval States Of Aquatic Invertebrates Following Pesticide Exposure, Karen Perry Favret

LSU Doctoral Dissertations

Current regulatory testing provides the basis for determining acceptable levels of pollutants in the environment, yet these acceptable levels of contaminants have resulted in undesirable consequences to organisms. The purpose of this dissertation was to test the hypothesis that biomarkers of cellular stress could be detected from sub-lethal exposure to pesticides in sperm and early life stages of broadcast spawning invertebrates. Exposures were conducted on oyster (Crassostrea virginica), mussel (Mytilus galloprovincialis and Dreissena polymorpha), and sea urchin (Lytechinus variegatus) embryos and larvae for 4 and 24 h to Bayluscide® and Roundup®. DNA fragmentation, a characteristic of apoptotic cells, was detected …

Essays On Environmental Issues Associated With The Diary Production Region In Louisiana, Larry Michael Hall

LSU Doctoral Dissertations

The Louisiana dairy production region (LDPR) is located in southeast Louisiana and includes five parishes (Tangipahoa, Washington, Livingston, St. Helena, and St. Tammany). It is home to approximately 90% of the state’s dairy industry and is a major contributor to nonpoint source pollution (NPS) in the area’s watershed through nitrogen, phosphorus, and sediment effluents. While point source (PS) effluents are easily identified and subject to federal and state regulations, NPS mitigation efforts must address uncertainties such as location of the NPS as well as stochastic parameters such as rainfall and its effect on nutrient and sediment flow. This dissertation presents …

Pore-Scale Analysis Of Dnapl Dissolution And Biomass Distribution, Keegan L. Roberts

LSU Doctoral Dissertations

A comparison of equilibrium and non-equilibrium dissolution of tetrachloroethylene (PCE) was conducted to ascertain how PCE saturation, individual blob properties (volume, surface area, sphericity), and PCE occupied pores are affected by two distinct dissolution regimes. One-dimensional columns were imaged at various dissolution stages using high resolution (~10 um) synchrotron x-ray tomography (XT) and image subvolumes were analyzed using a series of grain, pore network structure, and blob analysis algorithms. An analysis of algorithm-generated data was conducted to determine grain and pore statistics, PCE saturation and individual blob properties, and correlations between PCE blobs and pore network structure. Grain and pore …

The Isolation And Characterization Of Bovine Adult Derived Adipose Stem Cells For The Use In Nuclear Transfer, Alicia A. Picou

LSU Master's Theses

Since the cloning of Dolly there has been little change in the efficiency of nuclear transfer (NT). Research is beginning to investigate the characteristics of donor cells. Adiposetissue is an abundant source of adult-derived cells that have displayed “stemness” in-vitro(Gimble et al., 2003). The overall goal of this research was to define the in-vitro characteristicsof bovine adipose-derived adult stem cells (ADAS) for the use in NT. Isolation methods weredetermined by a 3 x 3 factorial design. 1 g of subcutaneous fat was collected and subjected to0.10%, 0.25% or 0.50% collagenase type I solution for 1, 2 and 3 h. Nucleated …

Translocation Success Of Adult Red-Cockaded Woodpeckers, Erin Herbez

LSU Master's Theses

The red-cockaded woodpecker (Picoides borealis; hereafter RCW) is a cooperative breeder endemic to open old-growth pine savannah ecosystems in the southeastern United States (Jackson 1994). The RCW was listed as federally endangered in 1973, after a population decline due to habitat loss. Habitat fragmentation produces isolated populations of RCWs, which in turn ultimately limits the success of the species. RCW biologists and managers counteract effects of fragmentation by aggregating recruitment clusters and translocation. Although several studies examined subadult RCW translocation, detailed studies examining adult translocation have been limited. My study was conducted on a 3500 ha area owned by Plum …

The Assessment Of And Differences Among Intellectually Disabled Adults With Comorbid Autism Spectrum Disorders And Epilepsy, Kimberly Robin Michelle Smith

LSU Doctoral Dissertations

The goal of this study was to systematically examine group differences among those with intellectual disabilities (ID) and comorbid Autism Spectrum Disorders (ASD) and epilepsy in an adult population through a detailed exploration of the characteristics that these disorders present in the areas of psychopathology, behavior problems, and impaired social behavior. Previous studies indicating that individuals with ID have comorbid ASD and neurological conditions tend to stop short of addressing these disorders’ impact on the full range of psychosocial issues, particularly in adult samples. Assessments of psychopathology, behavior problems, and impaired social behavior were made with the ASD-Comorbidity-Adult Version and …

Antiviral Chemotherapeutic Agents Against Equine Herpesvirus Type 1: The Mechanism Of Antiviral Effects Of Porphyrin Derivatives, Galena V. Rybachuk

LSU Doctoral Dissertations

Equine Herpesvirus-1 (EHV-1) is an important ubiquitous enzootic equine pathogen, causing significant economic losses to the horse industry. Despite extensive vaccination protocols, EHV-1 continues to be a major cause of epidemic abortion, perinatal mortality, respiratory disease and neurologic disease. EHV-1 infections are usually dealt with by using management practices that limit spread of the disease and secondary complications, providing symptomatic relief to infected horses, but no specific treatment is available. New therapeutic or virucidal agents could have great utility in slowing both the progression and spread of the disease in an epidemic situation. A number of porphyrins and their derivatives …

Heidegger And Dewey: Science In A Post-Metaphysical World, Michael Allen Dusch

LSU Master's Theses

The aim of this paper is to compare and contrast the philosophies of Martin Heidegger and John Dewey regarding metaphysics and science. Concerning metaphysics, there are two main areas of attention, the historical emergence of metaphysics in the philosophical tradition, and the abandonment of the metaphysical notions of substance and subjectivity. After examining these two areas of both thinkers’ philosophies, we then turn to their philosophy of science, demonstrating how the scientific thought of both thinkers is inseparable from their metaphysical critiques. For Heidegger, modern science is merely a continuation of the metaphysics of the past, while for Dewey; science …

A Critical Examination Into Motivation And Gender In Youth Physical Fitness Testing, Elizabeth A. Domangue

LSU Doctoral Dissertations

Fitness testing in physical education classes has emerged as an important component in efforts to assess and address health concerns related to children’s physical inactivity. To date, motivational aspects of fitness testing, especially in regard to students’ future intention to participate in fitness-related activities, have not been closely examined. Fitness testing practices have been identified as a dominant discourse in physical education that continue to separate girls’ and boys’ physicalities along gendered lines. This lack of understanding about the motivational implications of fitness testing programs, coupled with concerns related to the gendered nature of fitness testing, emphasize the significance of …

Studies On The Effect Of Natural Organic Matter And Hydration On The Sorption And Desorption Of Trifluorinated Pesticides, Charisma Vinarao Lattao

LSU Doctoral Dissertations

This work was done in order to deepen our molecular level understanding of how soil organic matter (SOM) is assembled in a whole soil and to provide further insight on the effect of SOM assembly on the uptake and release of hydrophobic organic compounds. Various techniques, including ultraviolet absorbance, fluorescence, and total carbon analysis, demonstrate that hydration/solvation of SOM is kinetically controlled. Initial wetting of a soil releases the hydrophobic moieties that are located at the outer surface of SOM, and longer wetting times exfoliate more hydrophobic quinone type moieties that are present in the middle layer, which in turn …

Asynchronous Replication Of Metadata Across Multi-Master Servers In Distributed Data Storage Systems, Ismail Akturk

LSU Master's Theses

In recent years, scientific applications have become increasingly data intensive. The increase in the size of data generated by scientific applications necessitates collaboration and sharing data among the nation's education and research institutions. To address this, distributed storage systems spanning multiple institutions over wide area networks have been developed. One of the important features of distributed storage systems is providing global unified name space across all participating institutions, which enables easy data sharing without the knowledge of actual physical location of data. This feature depends on the ``location metadata'' of all data sets in the system being available to all …

Investigation Into The Hydrothermal Treatment Of Sugarcane Bagasse Under Near- And Supercritical Conditions, John E. White

LSU Doctoral Dissertations

Processing biomass in near- and supercritical water has garnered increasing attention because of its ability to accept a variety of wet feedstocks, energy efficiency, and ability to regulate the solubility and separation of components. Very few studies of biomass hydrothermal conversion provide a comprehensive evaluation of multiple process parameters and various additives and their effects on a single product phase. This research examined the influence of temperature, residence time, biomass concentration, and particle size on volatiles production from the hydrothermal conversion of sugarcane bagasse. Temperature had the greatest impact on volatile yields with the largest increase (23 wt %) occurring …

Supercritical Fluid Extraction And Analysis Of Plant Oils, Tianchuan Du

LSU Master's Theses

Supercritical fluid extraction (SFE) of oxeye daisy (Chrysanthemum leucanthemum L.) seed, heartwood of Port-Orford cedar (POC) (Chamaecyparis lawsoniana), Alaska yellow cedar (AYC) (Chamaecyparis nootkatensis), and Eastern red cedar (ERC) (Juniperus virginiana L), and Chinese tallow (Sapium sebiferum L. Roxb. ) seed was investigated in this study and compared with other extraction methods. For the oxeye daisy seed extraction, Soxhlet extraction (SE) with hexane, microwave assisted extraction (MAE), and supercritical fluid extraction (SFE) were conducted. The results showed that as the extraction temperature decreased from 100 °C, the extraction rate increased until 30 °C using SFE. With an increase of extraction …

Scanning Probe Microscopy Investigations Of (1) Arrays Of Cysteine-Coated Cds Nanoparticles And (2) Structures Formed During The Early Stages Of The Corrosion Of Copper Surfaces, Brian Robert Lewandowski

LSU Doctoral Dissertations

Scanning probe microscopy (SPM) characterizations are becoming more prevalent for surface investigations due to their capabilities for obtaining structural information and physical measurements. New capabilities of SPM for studying and controlling nanoscale processes are emerging as valuable assets in research. A fundamental understanding of the interactions of surface reactions provides essential information for developing workable applications for nanotechnology. Two applications of SPM are discussed in this dissertation. The first investigation uses atomic force microscopy (AFM) for the characterization of nanostructures produced with a newly developed lithographic technique called “two-particle” lithography. This new technique is based on particle lithography for the …

Photocatalytic Reaction In Monolithic Optical Fiber Reactor With Inverse Opal Catalyst, Maoming Ren

LSU Doctoral Dissertations

The development of photocatalytic reactor is essential for the successful application of heterogeneous semiconductor in environmental study, which has been shown to be photoactive and effective to oxidate organic pollutant and photoreduce CO2 to useful compounds. In this dissertation, a monolithic optical fiber reactor (MOFR) coated with inverse opal titania, which uses optical fibers as light-transmitting conductor and support of catalyst, was developed for both photodegradation and photoreduction. 1,2-dichlorobenzene, a volatile organic compound (VOC), was selected as the organic pollutant. This configuration of reactor and catalyst provides a high surface area, enhances mass transfer within the catalyst, manipulates photons transmission …

A Multi-Function, Disposable, Microfluidic Module For Mutation Detection, Tae Yoon Lee

LSU Doctoral Dissertations

Recognition of point mutations in a codon 12 of the K-ras gene, most frequently observed, is considered to be useful in the early diagnosis of several types of the human cancers. We have developed a multifunction, disposable, microfluidic module which detects low-abundant point mutations in human genomic DNA in modular architecture. Each functional component including a microfluidic PCR reactor, a passive diffusional micromixer reactor, and a microfluidic LDR reactor was separately designed and fabricated. Fluidic interconnects were also developed to make a fluidic passage between the functional components. Polycarbonate substrates were micro-molded, using hot embossing with micro-milled brass mold inserts …

Collpas As Activity Hotspots For Frugivorous Bats (Stenodermatinae) In The Peruvian Amazon: Underlying Mechanisms And Conservation Implications, Adriana Bravo Ordonez

LSU Doctoral Dissertations

In western Amazonia, large numbers of frugivorous bats regularly visit natural forest clearings known locally as collpas (also called clay licks or mineral licks). Bats arrive at collpas to drink water that has accumulated in depressions created by larger mammals that consume soil. Although collpa visitation by bats appears relatively common in western Amazonia, little is known about its causes and its ecological implications. In this dissertation I describe general and seasonal patterns of collpa visitation by frugivorous bats in the Peruvian Amazon, and I investigate potential explanations for this unique behavior. Regardless of season, collpas seem to be activity …

Testicular Toxicity And The Potential For 1, 2 -Ethlyene Dichloride (Edc) To Initiate Epigenetic Disruption Of The Paternal Genome, Harold J. Daigle

LSU Doctoral Dissertations

Our genetic inheritance begins with one genome copy from each parent. The presence of one or more errors in either the maternal or the paternal genome can lead to genetic disease or disruption of the embryonic program and potential loss of the offspring. Numerous chemical and physical toxicants are known to produce germ line mutagenesis based on their ability to produce DNA sequence mutations. Exposures to these cytotoxic and mutagenic agents pose a risk for human offspring. However, very little is known regarding the sensitivity of the epigenetic patterns involved in reproductive functions to adverse effects of chemical agents. The …

Tnf-Alpha Inhibition Of Adiponectin Expression By Targeting Ppar-Gamma And C/Ebp In Adipocytes, Yanning Wang

LSU Master's Theses

Chronic inflammation is involved in the adipose tissue dysfunction through regulation of endocrine and storage function of adipocytes. As a representative proinflammatory cytokine, TNF-α was reported to inhibit expression of adiponectin. However, the mechanism of inhibition remains to be identified. Here, we provide experimental evidence that TNF-α inhibits adiponectin at both transcriptional and posttranscriptional levels. In three animal models (aP2-P65, ob/ob and high fat diets-fed mice), an increase in TNF-α expression was associated with a decrease in adiponectin expression. In 3T3-L1 adipocytes, TNF-α inhibition of adiponectin was observed at mRNA and protein levels. Luciferase reporter assay and mRNA stability tests …

Sialic Acid Conjugated Chitosan For The Attenuation Of Amyloid-Beta Toxicity, Dhruva Dilip Dhavale

LSU Master's Theses

Amyloid-beta (Aβ), a 39 to 43 amino acid long peptide, is the primary species identified in senile plaques associated with Alzheimer’s disease (AD) and has been implicated in the neurotoxicity associated with AD. It is believed that Aβ toxicity is mediated through the interaction with neuronal membranes. A variety of evidence indicates that 1) Aβ may bind to the cell surface sialic acids, 2) the affinity of this interaction is higher if the gangliosides or sialic acids on the cell surface are clustered, 3) the removal of the surface sialic acids attenuate Aβ toxicity. Based on this data, we hypothesized …

Atom-Based Computer Simulation Studies Of Gas-To-Liquid Nucleation In Atmospherically Relevant Systems: Clarifying Discrepancies And Elucidating Mechanisms, Ricky Bendanillo Nellas

LSU Doctoral Dissertations

For over a century, nucleation for all systems was thought simplistically to be a process that advances through the formation of critical clusters with a well-defined composition. Our results show intriguing nucleation mechanisms that challenge the aforementioned notion. We employed the simple TraPPE-UA (transferable potential for phase equilibria – united atom) force field and the AVUS-HR approach (a combination of aggregation-volume-bias Monte Carlo, umbrella sampling, and histogram reweighting), to investigate the homogeneous vapor-to-liquid nucleation of various nucleating systems. We found out that these systems could nucleate through a variety of unique non-ideal mechanisms. Alongside existing experimental investigations, this dissertation presents …

Intracellular Ice Formation In Adult Stem Cells In The Presence Of Polyvinyl Pyrrolidone, Avishek Guha

LSU Master's Theses

The main objective of this work was to assess the effect of 10% (w/v) polyvinylpyrrolidone (PVP) on the pattern of intracellular ice formation (IIF) in human adipose tissue derived adult stem cells (ASCs) in the absence of serum and other cryoprotective agents (CPAs). Passage 1 (P1) ASCs were cultured, washed and suspended in either 1x PBS (Phosphate Buffered Saline) or 10% w/v solution of PVP in 1x PBS. The freezing experiments were carried out using a fluorescence microscope equipped with a Linkam™ cooling stage using two different temperature/time cooling protocols. Both the cooling protocols had a common cooling ramp: cells …

Seasonal Growth And Shading Potential Of Sugarcane (Saccharum Spp. Hybrids) And Shade Response Of Perennial Weeds, Mariana Ferreira Bittencourt

LSU Master's Theses

Seasonal growth characteristics and shading ability of six sugarcane varieties were compared during the second production year. For each variety, trend analysis of data showed a significant linear trend. Differences in shoot emergence among the varieties were observed beginning in early April and as the season progressed, shoot production and shoot height increased for ‘L 97-128’, ‘Ho 95-988’, ‘L 99-226’, and ‘L 99-233’ but lagged for ‘HoCP 96-540’ and ‘LCP 85-384’. Beginning in mid-April ground cover increased most rapidly for L 99-233. In late May ground cover was around 60% for LCP 85-384 and HoCP 96-540; around 70% for L …

Cbod₅ Treatment And Nitrogen Transformations Of The Marshland Upwelling System In Intermediate And Saltwater Marshes, Lorna Anne Putnam

LSU Doctoral Dissertations

The marshland upwelling system (MUS) was designed to treat domestic wastewater from coastal dwellings where conventional methods are inadequate due to high water tables, poor hydraulic soil conditions, anaerobic soils, and saline groundwater. Currently there is no adequate treatment system available and coastal dwellings are contributing to water quality problems. This study focused on determining the treatment effectiveness of the MUS for organic matter and understanding the specific processes involved in nitrogen treatment.

The treatment of organic matter, measured as five-day carbonaceous biological oxygen demand (CBOD₅), was effective in field tests for both saltwater and intermediate marshes. Global …

Characterization Of Endoreplicated Nuclei In Arabidopsis Thaliana, Rasheed A. Ahmad

LSU Master's Theses

A model plant, Arabidopsis thaliana, duplicates its chromosomes without cell division, in a process known as endoreplication. The primary objective of this study was to identify genes and proteins that specifically accumulate in endoreplicated nuclei in Arabidopsis thaliana. Analysis of nuclei sorted by fluorescent activated cell sorter (FACS) revealed that ploidy levels and nuclear volume were positively correlated. However, the protein amounts among 2C, 4C, and 8C nuclei had no significant differences. This indicated a decrease of the protein concentration in endoreplicated nuclei. In contrast, the RNA amount in the 8C nuclei was 1.3- and 1.4-fold higher than that in …