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2009

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Wus And Stm-Based Reporter Genes For Studying Meristem Development In Poplar, Y. Bao, P. Dharmawardhana, R. S. Arias, M. B. Allen, C. Ma, Steven H. Strauss Jan 2009

Wus And Stm-Based Reporter Genes For Studying Meristem Development In Poplar, Y. Bao, P. Dharmawardhana, R. S. Arias, M. B. Allen, C. Ma, Steven H. Strauss

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We describe the development of a reporter system for monitoring meristem initiation in poplar using promoters of poplar homologs to the meristem-active regulatory genes WUSCHEL (WUS) and SHOOTMERISTEMLESS (STM). When ~3 kb of the 5' flanking regions of close homologs were used to drive expression of the GUSPlus gene, 50–60% of the transgenic events showed expression in apical and axillary meristems. However, expression was also common in other organs, including in leaf veins (40 and 46% of WUS and STM transgenic events, respectively) and hydathodes (56% of WUS transgenic events). Histochemical GUS staining of explants during callogenesis and shoot …


Particle Size Effects On The Quality Of Flour Tortillas Enriched With Whole Grain Waxy Barley, Naruemon Prasopsunwattana, Marfa Botero Omary, Elizabeth A. Arndt, Peter H. Cooke, Rolando A. Flores, Wallace Yokoyama, Ayako Toma, Sutida Chongcham, Sylvia P. Lee Jan 2009

Particle Size Effects On The Quality Of Flour Tortillas Enriched With Whole Grain Waxy Barley, Naruemon Prasopsunwattana, Marfa Botero Omary, Elizabeth A. Arndt, Peter H. Cooke, Rolando A. Flores, Wallace Yokoyama, Ayako Toma, Sutida Chongcham, Sylvia P. Lee

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Wheat tortillas were enriched with whole barley flour (WBF) of different particle sizes including 237 µm (regular [RD. 131 µm (intermediate [IM]). and 68 µm (microground [MG], Topographical and fluorescent microstructure images of flours, doughs, and tortillas were examined, Flours and tortillas were analyzed for color, protein, ash, starch, moisture. and β-glucan content. Farinograph testing was conducted on the flour blends, Water activity and texture analyses of tortillas were conducted, A 9-point hedonic scale was used by 95 untrained panelists to evaluate tortilla appearance, color, flavor, texture, and overall acceptability, Two commercial products (CP) were included in …


Reproductive And Sphingolipid Metabolic Effects Of Fumonisin B1 And Its Alkaline Hydrolysis Product In Lm/Bc Mice: Hydrolyzed Fumonisin B1 Did Not Cause Neural Tube Defects, Kenneth A. Voss, Ronald T. Riley, Maurice E. Snook, Janee Gelineau-Van Waes Jan 2009

Reproductive And Sphingolipid Metabolic Effects Of Fumonisin B1 And Its Alkaline Hydrolysis Product In Lm/Bc Mice: Hydrolyzed Fumonisin B1 Did Not Cause Neural Tube Defects, Kenneth A. Voss, Ronald T. Riley, Maurice E. Snook, Janee Gelineau-Van Waes

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Fumonisins are mycotoxins produced by Fusarium verticillioides. They are toxic to animals and exert their effects through mechanisms involving disruption of sphingolipid metabolism. Fumonisins are converted to their hydrolyzed analogs by alkaline cooking (nixtamalization). Both fumonisins and hydrolyzed fumonisins are found in nixtamalized foods such as tortillas, and consumption of tortillas has been implicated as a risk factor for neural tube defects (NTD). Fumonisin B1 (FB1) induced NTD when given (ip) to pregnant LM/Bc mice; however, neither the NTD induction potential of hydrolyzed fumonisin B1 (HFB1) nor its affect on sphingolipid metabolism in …


Effects Of Spontaneous Heating On Fiber Composition, Fiber Digestibility, And In Situ Disappearance Kinetics Of Neutral Detergent Fiber For Alfalfa-Orchardgrass Hays, W. K. Coblentz, P. C. Hoffman Jan 2009

Effects Of Spontaneous Heating On Fiber Composition, Fiber Digestibility, And In Situ Disappearance Kinetics Of Neutral Detergent Fiber For Alfalfa-Orchardgrass Hays, W. K. Coblentz, P. C. Hoffman

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

During 2006 and 2007, forages from 3 individual hay harvests were utilized to assess the effects of spontaneous heating on concentrations of fiber components, 48-h neutral detergent fiber (NDF) digestibility (NDFD), and in situ disappearance kinetics of NDF for large-round bales of mixed alfalfa (Medicago sativa L.) and orchardgrass (Dactylis glomerata L.). Over the 3 harvests, 96 large-round bales were made at preset bale diameters of 0.9, 1.2, or 1.5 m, and at moisture concentrations ranging from 9.3 to 46.6%. Internal bale temperatures were monitored daily during an outdoor storage period, reaching maxima (MAX) of 77.2°C and 1,997 heating degree …


A Whole-Genome Assembly Of The Domestic Cow, Bos Taurus, Aleksey V. Zimin, Arthur L. Delcher, Liliana Florea, David R. Kelley, Michael C. Schatz, Daniela Puiu, Finnian Hanrahan, Geo Pertea, Curtis P. Van Tassell, Tad S. Sonstegard, Guillaume Marçais, Michael Roberts, Poorani Subramanian, James A. Yorke, Steven. L Salzberg Jan 2009

A Whole-Genome Assembly Of The Domestic Cow, Bos Taurus, Aleksey V. Zimin, Arthur L. Delcher, Liliana Florea, David R. Kelley, Michael C. Schatz, Daniela Puiu, Finnian Hanrahan, Geo Pertea, Curtis P. Van Tassell, Tad S. Sonstegard, Guillaume Marçais, Michael Roberts, Poorani Subramanian, James A. Yorke, Steven. L Salzberg

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Background: The genome of the domestic cow, Bos Taurus, was sequenced using a mixture of hierarchical and whole-genome shotgun sequencing methods.

Results: We have assembled the 35 million sequence reads and applied a variety of assembly improvement techniques, creating an assembly of 2.86 billion base pairs that has multiple improvements over previous assemblies: it is more complete, covering more of the genome; thousands of gaps have been closed; many erroneous inversions, deletions, and translocations have been corrected; and thousands of single-nucleotide errors have been corrected. Our evaluation using independent metrics demonstrates that the resulting assembly is substantially more accurate …


Expression And Functional Characterization Of The Plant Antimicrobial Snakin-1 And Defensin Recombinant Proteins, Natalia Kovalskaya, Rosemarie W. Hammond Jan 2009

Expression And Functional Characterization Of The Plant Antimicrobial Snakin-1 And Defensin Recombinant Proteins, Natalia Kovalskaya, Rosemarie W. Hammond

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In this study, for the first time, functionally active, recombinant, cysteine-rich plant proteins snakin-1 (SN1) and defensin (PTH1) were expressed and purified using a prokaryotic expression system. The overall level of antimicrobial activities of SN1 and PTH1 produced in Escherichia coli was commensurate with that of the same proteins previously obtained from plant tissues. Both proteins exhibited strong antibacterial activity against the phytopathogenic bacterium Clavibacter michiganensis subsp. sepedonicus (50% inhibitory concentration (IC50) 1.5–8 lM) and antifungal activity against the phytopathogenic fungi Colletotrichum coccoides and Botrytis cinerea (IC50 5–14 lM). Significantly weaker activity was observed against Pseudomonas syringae …


Open Field Host Selection And Behavior By Tamarisk Beetles (Diorhabda Spp.) (Coleoptera: Chrysomelidae) In Biological Control Of Exotic Saltcedars (Tamarix Spp.) And Risks To Non-Target Athel (T. Aphylla) And Native Frankenia Spp., Patrick J. Moran, C. Jack Deloach, Tom L. Dudley, Joaquin Sanabria Jan 2009

Open Field Host Selection And Behavior By Tamarisk Beetles (Diorhabda Spp.) (Coleoptera: Chrysomelidae) In Biological Control Of Exotic Saltcedars (Tamarix Spp.) And Risks To Non-Target Athel (T. Aphylla) And Native Frankenia Spp., Patrick J. Moran, C. Jack Deloach, Tom L. Dudley, Joaquin Sanabria

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Biological control of invasive saltcedars (Tamarix spp.) in the western U.S. by exotic tamarisk leaf beetles, Diorhabda spp., first released in 2001 after 15 years of development, has been successful. In Texas, beetles from Crete, Greece were first released in 2004 and are providing control. However, adults alight, feed and oviposit on athel (Tamarix aphylla), an evergreen tree used for shade and as a windbreak in the southwestern U.S. and México, and occasionally feed on native Frankenia spp. plants. The ability of tamarisk beetles to establish on these potential field hosts was investigated in the field. In …


Efficacy And Safety Of Catnip (Nepeta Cataria) As A Novel Filth Fly Repellent, J. J. Zhu, X.-P. Zeng, D. Berkebile, H.-J. Du, Y. Tong, K. Qian Jan 2009

Efficacy And Safety Of Catnip (Nepeta Cataria) As A Novel Filth Fly Repellent, J. J. Zhu, X.-P. Zeng, D. Berkebile, H.-J. Du, Y. Tong, K. Qian

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Catnip (Nepeta cataria) is known for its pseudo-narcotic effects on cats. Recently, it has been reported as an effective mosquito repellent against several Aedes and Culex species, both topically and spatially. Our laboratory bioassays showed that catnip essential oil (at a dosage of 20 mg) resulted in average repellency rates of 96% against stable flies, Stomoxys calcitrans (L.) and 79% against houseflies, Musca domestica (L.), respectively. This finding suggested that the application of repellent could be used as part of filth fly management. Further evaluations of catnip oil toxicity were conducted to provide a broad-spectrum safety profile of …


Physiological And Environmental Regulation Of Interannual Variability In Co2 Exchange On Rangelands In The Western United States, H. Wayne Polley, William Emmerich, James A. Bradford, Phillip L. Sims, Douglas H. Johnson, Nicanor Z. Saliendra, Tony Svejcar, Raymond Angell, Albert B. Frank, Rebecca L. Phillips, Keirith A. Snyder, Jack A. Morgan Jan 2009

Physiological And Environmental Regulation Of Interannual Variability In Co2 Exchange On Rangelands In The Western United States, H. Wayne Polley, William Emmerich, James A. Bradford, Phillip L. Sims, Douglas H. Johnson, Nicanor Z. Saliendra, Tony Svejcar, Raymond Angell, Albert B. Frank, Rebecca L. Phillips, Keirith A. Snyder, Jack A. Morgan

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

For most ecosystems, net ecosystem exchange of CO2 (NEE) varies within and among years in response to environmental change.We analyzed measurements of CO2 exchange from eight native rangeland ecosystems in the western United States (58 site-years of data) in order to determine the contributions of photosynthetic and respiratory (physiological) components of CO2 exchange to environmentally caused variation in NEE. Rangelands included Great Plains grasslands, desert shrubland, desert grasslands, and sagebrush steppe.


Public Bioethics And The Bush Presidency, O. Carter Snead Jan 2009

Public Bioethics And The Bush Presidency, O. Carter Snead

Journal Articles

Public bioethics figured prominently during the tenure of President George W. Bush. This Article explores the Bush legacy in this domain. It begins by articulating and examining the grounding norms of President Bush’s approach to public bioethics. Next, it analyzes how these norms were applied to concrete areas of concern. Building on this analysis, the next section reflects on what the President’s actions illustrate about the capacity of the Executive Branch to shape public bioethics. The Article concludes with a brief discussion of the possible metrics by which the Bush Administration’s efforts might be judged, and then offers several assessments …


The Effectiveness Of Biodiversity Law, John Copeland Nagle Jan 2009

The Effectiveness Of Biodiversity Law, John Copeland Nagle

Journal Articles

The Endangered Species Act (ESA) has generated a heated debate between those who believe that the law has succeeded and those who believe that the law has failed. The resolution to that debate depends upon whether the law’s stated purposes or some other criteria provide the basis for judging a law’s effectiveness. Meanwhile, since the enactment of the ESA in 1973, biodiversity protection has received growing attention in the nations of southeastern Asia. So far, the law has been much less effective in protecting Asian biodiversity from habitat loss, commercial exploitation, and other threats, yet southeastern Asia’s biodiversity law has …


H.L.A. Hart: A Twentieth-Century Oxford Political Philosopher, John M. Finnis Jan 2009

H.L.A. Hart: A Twentieth-Century Oxford Political Philosopher, John M. Finnis

Journal Articles

This essay offers first a sketch (by a student and colleague) of H.L.A. Hart's life; second an account of the political philosophy which he explicitly articulated in The Concept of Law (1961), and of its relation to the main currents of Oxford political philosophy in the 1950s; and thirdly an exposition and critical assessment of the normative political theory deployed, to widespread acclaim, in his Law, Liberty & Morality (1963).


Vegetation Of Utah, R. Douglas Ramsey, Neil E. West Jan 2009

Vegetation Of Utah, R. Douglas Ramsey, Neil E. West

Wildland Resources Faculty Publications

In order to organize and facilitate management of Utah’s ecosystems, generalization of the ecological variation found across the state is necessary. Ecosystems involve complex interactions between environment and biota, and there have been many efforts to generalize and categorize these interactions in order to gain a better understanding of their structure and organization. The most common method of categorizing ecological variations across large landscapes today incorporates the ecoregion concept. Ecoregions are geographic delineations of landscapes containing ecosystems linked by similar climatic, geologic, soil, and landform characteristics. The primary characteristics used to delineate ecoregions vary depending on the overall goal of …


Molecular Monitoring Of African Swine Fever Control Areas: Adult-Tick Targeted Re-Evaluation Of Mkuze Game Reserve, South Africa, L. F. Arnot, Johan T. Du Toit, A. D.S. Bastos Jan 2009

Molecular Monitoring Of African Swine Fever Control Areas: Adult-Tick Targeted Re-Evaluation Of Mkuze Game Reserve, South Africa, L. F. Arnot, Johan T. Du Toit, A. D.S. Bastos

Wildland Resources Faculty Publications

The Mkuze Game Reserve (MGR), in north-eastern KwaZulu-Natal Province, South Africa is an African swine fever virus (ASF) controlled area. In a survey conducted in 1978, ASF prevalence in warthogs and Ornithodoros ticks in MGR was determined to be 2% and 0.06%, respectively. These values, acknowledged as being unusually low compared to other East and southern African ASF-positive sylvatic-cycle host populations, have not been assessed since. The availability of a sensitive PCR-based virus detection method, developed specifically for the sylvatic tampan host, prompted a re-evaluation of ASF virus (ASFV) prevalence in MGR ticks. Of the 98 warthog burrows inspected for …


Staphylococcus Aureus Activates Type I Ifn Signaling In Mice And Humans Through The Xr Repeated Sequences Of Protein A, Francis J. Martin, Marisa I. Gomez, Dawn M. Wetzel, Guido Memmi Jan 2009

Staphylococcus Aureus Activates Type I Ifn Signaling In Mice And Humans Through The Xr Repeated Sequences Of Protein A, Francis J. Martin, Marisa I. Gomez, Dawn M. Wetzel, Guido Memmi

Dartmouth Scholarship

No abstract provided.


Sexual Attitudes And Perceptions Of Gendered Sexual Roles From The Perspective Of Urban South African Women, Duduzile Phindile Mashinini Jan 2009

Sexual Attitudes And Perceptions Of Gendered Sexual Roles From The Perspective Of Urban South African Women, Duduzile Phindile Mashinini

All NMU Master's Theses

No abstract provided.


Analysis And Quantitation Of The Cross Presentation Of Tumor Antigens Using The Hiv Protein Transduction Domain Transactivating Regulatory Protein (Tat) To Alter Presentation, Jason Paul Aun Jan 2009

Analysis And Quantitation Of The Cross Presentation Of Tumor Antigens Using The Hiv Protein Transduction Domain Transactivating Regulatory Protein (Tat) To Alter Presentation, Jason Paul Aun

All NMU Master's Theses

No abstract provided.


Cease To Exit, Eric Edwin-Joseph Benac Jan 2009

Cease To Exit, Eric Edwin-Joseph Benac

All NMU Master's Theses

No abstract provided.


Effects Of Collaborative Action Research And Biology Journaling Notebooks On High School Student Achievement, Elsa Joan Clark Clement Jan 2009

Effects Of Collaborative Action Research And Biology Journaling Notebooks On High School Student Achievement, Elsa Joan Clark Clement

All NMU Master's Theses

No abstract provided.


Effect Of Antigenic Site Mutations On The Binding Specificity Of An Anti-Hemagglutinin Antibody To H3n2 Influenza Virus Isolates, Juliana Liambaya Hagembe Jan 2009

Effect Of Antigenic Site Mutations On The Binding Specificity Of An Anti-Hemagglutinin Antibody To H3n2 Influenza Virus Isolates, Juliana Liambaya Hagembe

All NMU Master's Theses

No abstract provided.


Seasonal Changes In Growth And Thyroid Hormones In Lake Superior Brook Trout, Carla A. Serfas Jan 2009

Seasonal Changes In Growth And Thyroid Hormones In Lake Superior Brook Trout, Carla A. Serfas

All NMU Master's Theses

No abstract provided.


Optimizing Her2 Antigen Presentation In The Mhc I, Trisha Rene Sippel Jan 2009

Optimizing Her2 Antigen Presentation In The Mhc I, Trisha Rene Sippel

All NMU Master's Theses

No abstract provided.


The Comparison Of Co-Stimulatory Surface Molecule Expression On Human Monocyte-Derived Dendritic Cells Infected With Two Strains Of Influenza Virus, Jessie M. Simmon Jan 2009

The Comparison Of Co-Stimulatory Surface Molecule Expression On Human Monocyte-Derived Dendritic Cells Infected With Two Strains Of Influenza Virus, Jessie M. Simmon

All NMU Master's Theses

No abstract provided.


Evaluation Of The Trophic Ecology Of A Freshwater Sponge And Two Sponge Predators Via Stale Isotope Analyses, James Skelton Jan 2009

Evaluation Of The Trophic Ecology Of A Freshwater Sponge And Two Sponge Predators Via Stale Isotope Analyses, James Skelton

All NMU Master's Theses

No abstract provided.


Miss Those Men, Sarah J. Wangler Jan 2009

Miss Those Men, Sarah J. Wangler

All NMU Master's Theses

No abstract provided.


Blood Chimerism Confounds Genetic Relative Susceptibility Testing For Classical Scrapie In Sheep, David A. Schneider, Ahmed Tibary, Terje Taudsepp, Pranab J. Das, Katherine I. O'Rourke Jan 2009

Blood Chimerism Confounds Genetic Relative Susceptibility Testing For Classical Scrapie In Sheep, David A. Schneider, Ahmed Tibary, Terje Taudsepp, Pranab J. Das, Katherine I. O'Rourke

Zoonotics and Wildlife Disease: Publications

Classical scrapie disease is a transmissible spongiform encephalopathy of sheep that is enzootic in the United States. Susceptibility of sheep to classical scrapie is linked to single nucleotide polymorphisms in the prion protein gene (PRNP), forming the basis for genetic testing strategies used by national efforts to eradicate scrapie. Such efforts are occasionally hampered by inconclusive results stemming from the detection of ‘‘complex’’ genotypes. Naturally occurring cases of ovine chimerism are thought to account for some of these instances. In the current report, 4 naturally occurring ovine chimeras are documented through cytogenetic and molecular analyses. All 4 of these sheep …


Nor98 Scrapie Identified In The United States, Christie M. Loiacono, Bruce V. Thomsen, S. Mark Hall, Matti Kiupel, Diane Sutton, Katherine I. O'Rourke, Bradd Barr, Lucy Anthenill, Delwyn Keane Jan 2009

Nor98 Scrapie Identified In The United States, Christie M. Loiacono, Bruce V. Thomsen, S. Mark Hall, Matti Kiupel, Diane Sutton, Katherine I. O'Rourke, Bradd Barr, Lucy Anthenill, Delwyn Keane

Zoonotics and Wildlife Disease: Publications

A distinct strain of scrapie identified in sheep of Norway in 1998 has since been identified in numerous countries throughout Europe. The disease is known as Nor98 or Nor98-like scrapie, among other names. Distinctions between classic scrapie and Nor98 scrapie are made based on histopathology and immunodiagnostic results. There are also differences in the epidemiology, typical signalment, and likelihood of clinical signs being observed. In addition, sheep that have genotypes associated with resistance to classic scrapie are not spared from Nor98 disease. The various differences between classic and Nor98 scrapie have been consistently reported in the vast majority of cases …


Dna Delivery From Matrix Metalloproteinase Degradable Poly (Ethylene Glycol) Hydrogels To Mouse Cloned Mesenchymal Stem Cells, Yuguo Lei, Tatiana Segura Jan 2009

Dna Delivery From Matrix Metalloproteinase Degradable Poly (Ethylene Glycol) Hydrogels To Mouse Cloned Mesenchymal Stem Cells, Yuguo Lei, Tatiana Segura

Department of Chemical and Biomolecular Engineering: Faculty Publications

The ability to genetically modify mesenchymal stem cells (MSCs) seeded inside synthetic hydrogel scaffolds would offer an alternative approach to guide MSC differentiation and to study molecular pathways in three dimensions than protein delivery. In this report, we explored gene transfer to infiltrating MSCs into matrix metalloproteinase (MMP) degradable hydrogels that were loaded with DNA/poly(ethylene imine) (PEI) polyplexes. DNA/PEI polyplexes were encapsulated inside poly (ethylene glycol) (PEG) hydrogels crosslinked with MMP degradable peptides via Michael Addition chemistry. A large fraction of encapsulated polyplexes remained active after encapsulation (65%) and the mechanical properties of the hydrogels were unchanged by the encapsulation …


Diversity Congruency Within Organizations: The Relationship Among Emotional Intelligence, Personality Structure, Ethnic Identity, Organizational Context And Perceptions Of Organizational Diversity, David Hurlic Jan 2009

Diversity Congruency Within Organizations: The Relationship Among Emotional Intelligence, Personality Structure, Ethnic Identity, Organizational Context And Perceptions Of Organizational Diversity, David Hurlic

Theses and Dissertations

This study explores the relationship among Emotional Intelligence (EI) (consisting of perception and appraisal, facilitating thinking, understanding emotions, and regulating emotions), Personality Structure (FFM) (consisting of extroversion, agreeableness, conscientiousness, neuroticism, and openness to experiences), Ethnic Identity, Organizational Context (OC), and Perceptions of Organizational Diversity. It is hoped that by examining the impact of individual personality structures, as well as, ethnic identity and organizational context as precursors to individual perceptions between EI, Personality Structure, and diversity and affirmative action, a more coherent method of analysis is created allowing organizations to identify specific factors contributing to affirmative action and diversity program acceptance …


A Clinicians' Guide To Integrating Mindfulness Into Evidence-Based Practice: A Common Elements Approach, Lara Fielding Jan 2009

A Clinicians' Guide To Integrating Mindfulness Into Evidence-Based Practice: A Common Elements Approach, Lara Fielding

Theses and Dissertations

Stemming from Eastern practices, mindfulness entails intentionally bringing one's attention to all aspects of experience in the present moment, and holding these experiences with non-judgmental acceptance. In recent years mindfulness-based interventions have been gaining empirical support together with clinician interest in their use. The APA now mandates the use of evidence-based practices in psychology (EBPP), which incorporate the best empirical evidence, clinical expertise, and individual population characteristics and culture. However, at present, because there have been relatively few dismantling studies, clinicians are forced to either adopt an entire treatment protocol or pick and choose individual interventions in a relatively ad …