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Functional Characterization Of Pag1, The Α7 Subunit Of The 20s Proteasome And Of The Ubiquitin-Specific Protease Subfamilies Ubp12/13 And Ubp3/4 In Arabidopsis Thaliana., Gulsum Soyler-Ogretim

Graduate Theses, Dissertations, and Problem Reports (ETD)

The ubiquitin/26S proteasome system (UPS) is conserved in all eukaryotic species. The 26S proteasome serves as the protease in this pathway and is responsible for the degradation of abnormal and short-lived proteins after their modification with a polyubiquitin tag. It consists of one or two ATP-dependent, 19S regulatory particles and an ATP-independent 20S core protease. PAG1 serves as one of at least 14 different subunits of the 20S core protease. In this study, the role of the 20S core protease in general and PAG1, the α7 subunit of the 26S proteasome, in particular during Arabidopsis growth and development was investigated …

Aging And Gender In The Coronary Microcirculation: Effects Of Oxygen And Hydrogen Peroxide., Lori S. Kang

Graduate Theses, Dissertations, and Problem Reports (ETD)

Study I examined the effects of aging and reactive oxygen species on flow-mediated vasodilation in coronary arterioles. Isolated coronary arterioles of young (4 mo) and aged (24 mo) male Fisher 344 rats were subjected to stepwise increases in flow (0–50 nL/sec) in the presence and absence of superoxide dismutase (SOD) mimetic Tempol (100 µM), hydrogen peroxide (H2 O2) scavenger catalase (100 U/mL), Tempol + catalase, or Tempol + deferoxamine (100 µM), an iron chelator and inhibitor of hydroxyl radical formation. Flow-induced nitric oxide (NO) and H2O 2 production decline with age, as measured by diaminofluorescein (DAF) and dichlorodihydrofluorescein (DCF) fluorescence, …

Pollution Swapping: The Effect Of Phosphorus Sequestration On Denitrification In Wetland Sediments, Nathaniel Femi Adegboyega

Dissertations and Theses @ UNI

Surface water quality is currently one of the most important environmental issues facing the state of Iowa since the ecological and recreational health of water bodies are threatened by non-point pollution. Most of Iowa's lakes are polluted because of their high concentration of nutrients, often phosphorus (P), which leads to excessive algal growth (eutrophication). To prevent eutrophication, it is necessary to prevent P from entering surface waters or to sequester (make unavailable to algae) the P that is already in the water body. One method to achieve this is to introduce substances that can tightly fix P (sorption).

Previous studies …

The Work Product Doctrine And Tax Accrual Workpapers, Steve R. Johnson

Scholarly Publications

In its rehearing of Textron, the First Circuit has an opportunity to rectify an error and curb unwise recent expansion of work product protection for tax accrual workpapers. In August 2007, a district court denied the government’s petition for enforcement of an IRS summons on Textron Inc. and its subsidiaries for tax accrual workpapers in connection with IRS examinations of the taxpayers’ 1998 to 2001 income tax returns. A divided panel of the First Circuit affirmed in January 2009. In March 2009, the First Circuit withdrew the panel opinion and dissent, and it set the case for en banc hearing …

Risk Management Of Commercial Overland Food Transport And Distribution, Lucas Charles Hartman Geadelmann

Dissertations and Theses @ UNI

The purpose of this research was to study the contemporary safety issues related to the overland transport of perishable food items via semi-truck and trailer. An anonymous survey instrument consisting of 20 questions was developed in order to examine semi-truck driver habits. The questions pertained to truck driver safety and subsequent food safety matters related to the perishable food items contained within the trailers. The paper surveys were distributed to the semi-truck driving employees of two food distributors located in the upper Midwest, which had agreed to voluntarily participate in the study. A total of 90 surveys were completed and …

Flipping The Script: Contra Proferentem And Standard Form Contracts, David Horton

University of Colorado Law Review

Virtually all modern contracts are standard forms. Although courts have long interpreted ambiguities in such agreements strictly against the drafter, they have struggled to explain why they do so. Under sustained academic fire, states are beginning to abandon the strict against-the-drafter doctrine. Recent cases have even refused to certify class actions on the grounds that a corporate defendant's nonnegotiated, unilaterally-dictated contract is ambiguous and thus cannot be construed without individualized extrinsic evidence. This Article claims that the rejection of the strict against-the-drafter rule stems from confusion about its normative foundation. Judges and commentators have offered three rationales for the doctrine: …

Toward A Net-Zero Carbon Planet: A Policy Proposal, Matthew J. Kiefer

University of Colorado Law Review

The effort to address climate change is global in scale and increasingly urgent, yet it lacks an effective policy framework. President Obama's determination to elevate clean energy to a national policy priority, Congress's consideration of a federal cap-and-trade regime for greenhouse gases, and the upcoming revisions to the Kyoto Protocol all provide an opportunity to move toward adopting a globally balanced carbon budget. A balanced carbon budget could replace the current, somewhat arbitrary greenhouse gas reduction targets with a scientifically derived calibration limiting global carbon emissions to the rate of carbon absorption. Carbon sub-budgets could then be allocated to each …

Custom-Made Helmet Fabrication For Occupational Therapists Treating Patients With Traumatic Brain Injury, Megan Schill, Michael Shae

Occupational Therapy Capstones

Soldiers within the Veteran's Administration who have sustained a traumatic brain injury (TBI) were the focus of this project. Reports showed a growing number of soldiers have returned from Iraq with head injuries, including cranial and brain defects (Defense and Veterans Brain Injury Center, 2007). Symptoms individuals with TBI commonly exhibit include dizziness, balance problems, sleep problems, excessive fatigue, headaches, and difficulties with cognitive processes. Other symptoms associated with a TBI are blurred vision, ringing in the ears, and bad tastes in the mouth (National Institute of Neurological Disorders and Stroke, 2007). The purpose of this project was to develop …

Electroporation For The Delivery Of Dna-Based Vaccines And Immunotherapeutics: Current Clinical Developments, Angela M. Bodles-Brakhop, Richard Heller, Ruxandra Draghia-Akli

Bioelectrics Publications

Electroporation (EP) has been used in basic research for the past 25 years to aid in the transfer of DNA into cells in vitro. EP in vivo enhances transfer of DNA vaccines and therapeutic plasmids to the skin, muscle, tumors, and other tissues resulting in high levels of expression, often with serological and clinical benefits. the recent interest in nonviral gene transfer as treatment options for a vast array of conditions has resulted in the refinement and optimization of EP technology. current research has revealed that EP can be successfully used in many species, including humans. clinical trials are …

Molecular Dynamics Study Of Single Stranded Peptide Nucleic Acids, Anna K. Manukyan

Theses and Dissertations in Biomedical Sciences

A PNA molecule is a DNA strand where the sugar-phosphate backbone has been replaced by a structurally homomorphous pseudopeptide chain consisting of N(2-aminoethyl)-glycine units. PNA binds strongly to both DNA and RNA. However, an analysis of the X-ray and NMR data show that the dihedral angles of PNA/DNA or PNA/DNA complexes are very different from those of DNA:DNA or RNA:RNA complexes. In addition, the PNA strand is very flexible. One way to improve the binding affinity of PNA for DNA/RNA is to design a more pre-organized PNA structure. An effective way to rigidify the PNA strand is to introduce …

Ecology And Molecular Phylogenetics Of Hydnora (Hydnoraceae) In Southern Africa, Jay Francis Bolin

Biological Sciences Theses & Dissertations

The Hydnoraceae are a clade of root holoparasitic angiosperms that contain two small genera, Hydnora and Prosopanche. This study, focused on Hydnora, presents novel data regarding the pollination biology, germination ecology, parasite-host nutritional relationships, and the molecular systematics of this group. Experimental addition of the primary pollinator, Dermestes maculatus to Hydnora africana chamber flowers demonstrated beetle imprisonment during the carpellate stage. Changes in the inner surfaces of the androecial chamber allowed beetle escape after pollen release. Most beetles escaped, dusted with viable pollen, three days after pollen release. To investigate germination ecology, aqueous root extracts of host and …

A System For Estimating Bowen Ratio And Evaporation From Waste Lagoons, Rezaul Mahmood

School of Natural Resources: Faculty Publications

No abstract provided.

Panarchy: Discontinuities Reveal Similarities In The Dynamic System Structure Of Ecological And Social Systems, Ahjond S. Garmestani, Craig R. Allen, Lance Gunderson

School of Natural Resources: Faculty Publications

In this paper, we review the empirical evidence of discontinuous distributions in complex systems within the context of panarchy theory and discuss the significance of discontinuities for understanding emergent properties such as resilience. Over specific spatial-temporal scale ranges, complex systems can configure in a variety of regimes, each defined by a characteristic set of self-organized structures and processes. A system may remain within a regime or dramatically shift to another regime. Understanding the drivers of regime shifts has provided critical insight into system structure and resilience. Although analyses of regime shifts have tended to focus on the system level, new …

Focal Acidosis In The Pre-Bötzinger Complex Area Of Awake Goats Induces A Mild Tachypnea, K. L. Krause, Hubert V. Forster, S. E. Davis, T. Kiner, Joshua M. Bonis, Lawrence Pan, B. Qian

Physical Therapy Faculty Research and Publications

There are widespread chemosensitive areas in the brain with varying effects on breathing. In the awake goat, microdialyzing (MD) 50% CO(2) at multiple sites within the medullary raphe increases pulmonary ventilation (Vi), blood pressure, heart rate, and metabolic rate (Vo(2)) (11), while MD in the rostral and caudal cerebellar fastigial nucleus has a stimulating and depressant effect, respectively, on these variables (17). In the anesthetized cat, the pre-Bötzinger complex (preBötzC), a hypothesized respiratory rhythm generator, increases phrenic nerve activity after an acetazolamide-induced acidosis (31, 32). To gain insight into the effects of focal acidosis (FA) within the preBötzC during physiological …

Beyond Aica Riboside: In Search Of New Specific Amp-Activated Protein Kinase Activators, Bruno Guigas, Kei Sakamoto, Nellie Taleux, Sara M. Reyna, Nicolas Musi, Benoit Viollet, Louis Hue

School of Medicine Publications

5-Aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICA riboside) has been extensively used in vitro and in vivo to activate the AMP-activated protein kinase (AMPK), a metabolic sensor involved in both cellular and whole body energy homeostasis. However, it has been recently highlighted that AICA riboside also exerts AMPK-independent effects, mainly on AMP-regulated enzymes and mitochondrial oxidative phosphorylation (OXPHOS), leading to the conclusion that new compounds with reduced off target effects are needed to specifically activate AMPK. Here, we review recent findings on newly discovered AMPK activators, notably on A-769662, a nonnucleoside compound from the thienopyridone family. We also report that A-769662 is able to activate …

Concert Recording 2009-03-27, Emily Nelson, Kristin Harwell, James Greeson

Concert Recordings & Programs

Orange dawn / Ian Clarke -- Sonatine for flute and piano / Henri Dutilleux -- L'aube enchantee / Ravi Shankar -- L'oiseau blesse / Denis Gougeon -- Renaissance concerto / Lukas Foss

Symbiotic Effects Of The Fungus Glomus Sp. On Chromium(Iii), Chromium(Vi), And Lead(Ii) Uptake By Mesquite (Prosopis Sp.): A Novel Method To Remediate Heavy Metals, Jack A. Arias

Open Access Theses & Dissertations

The indiscriminate disposal of lead (Pb) and chromium (Cr) containing wastes has resulted in a detrimental impact on environmental health. Lead and Cr are in the EPA priority list due to their degree of toxicity on humans, animals, plants, and other living organisms. The removal of toxic wastes involves technologies that are expensive, sometimes incomplete, and could represent health hazards. In addition, site restoration after contaminant removal encompasses time and technological resources. Phytoremediation, the use of plants alone or associated with microorganisms, represents a potentially cost-effective option in the long term management of contaminated sites. Various plants known as "hyperaccumulators" …

Uptake Of Pahs And Metals By Plants In Arid Environment, Zarhelia Carlo

Open Access Theses & Dissertations

Study of plant uptake of PAHs with the concurrent presence of metals in laboratory conditions was done with the aim to resemble specific combinations of natural environment. Toxicological response of seedlings was tested to define treatment conditions or possible interferences. The levels of PAHs and metals in the treatments were set up to have no toxic effect on plant growth. The complexity of concurrent contamination was demonstrated in a laboratory with soil. No visual differences were observed among various dose treatments of Cu and Zn with organics three PAHs, Phenanthrene (Phen), Acetoanthrancene, and Benzo(a)pyrene (BaP). The metal analysis by ICP/OES …

Ms.Ms., Amanda Elvira Cuellar

Open Access Theses & Dissertations

In this paper, the idea that Shakespeare's Caesar is just as Shavian as Shaw's representation of Julius Caesar is explored. In addition, an examination of the characteristics of the Life Force that prevail in Caesar and Cleopatra is presented. I compare these characteristics to the qualities described in Shakespeare's play. Ultimately, both rulers employ the power of the Life Force which exemplifies each man's genius.

Art In A New Light: Design And Assessment Of Illuminants To Reduce Photochemical Degradation Of Works Of Art, Monica Fabiola Delgado

Open Access Theses & Dissertations

The purpose of this research is to design the best lighting that will minimize long term photochemical degradation of Old Master Drawings and/or other works of art, while maintaining the patron's appreciation of the object's color and detail. The present approach is a technological refinement to the basic underlying earlier work on fluorescent lighting by W. A. Thornton, W.A. at General Electric1. Thin-film dielectric, multi-coating technology is used to create filters that eliminate ultraviolet light, near infrared light and significant unnecessary parts from the visible spectrum, thus maximizing the reduction in photochemical degradation, while maintaining optimal color rendering. Three interference …

The Association Of Dust Events With Asthma Exacerbation In The U.S.-Mexico Border Children: A Pilot Study, Monika Gaytan

Open Access Theses & Dissertations

Background. Asthma is one of the most prevalent chronic pediatric illnesses in the U.S. and a major cause of childhood disability, hospitalization, and missed schooldays. Air pollution is one of a number of known environmental triggers known to exacerbate pediatric asthma. Emerging evidence also suggests that exposure to dust aerosols associated with dust storm events may trigger asthmatic symptoms. The Paso del Norte (PDN) region is the single most dust-prone major metropolitan area in the U.S., averaging 14.5 major dust events/yr. The annual peak in dust event activity occurs during March to May where there is a 42% chance of …

The Evaluation Of A Web-Based Physical Activity Intervention In A Predominantly Hispanic College Population, Dejan Magoc

Open Access Theses & Dissertations

The American Heart Association (AHA) and the American College of Sports Medicine (ACSM) suggest at least 30 min of moderate physical activity at least 5 days a week or 20 min of vigorous physical activity at least 3 days a week. The overall aim of this experiment was to evaluate the efficacy of a web-based intervention - one that relied on existing, easy-to-use course technology - to increase days of weekly physical activity among predominantly Hispanic college students attending a large Southwest University. The principal hypothesis was that the intervention would significantly increase days of physical activity among those participating …

The Exquisite Pain Of Sunny Afternoons, Jessica Jeanne Miller

Open Access Theses & Dissertations

The Exquisite Pain of Sunny Afternoons is a novella exploring one young woman's journey through mental illness. She struggles with many delusions and extreme emotional reactions to these delusions until she finds balance. She finds balance, if not total recovery, through the help of her mother and three young Canadian travelers. The novella begins with an escape from a psychiatric hospital to Oaxaca, Mexico.

Cis-Acting Signals For Replication Of Nodamura Virus Rna1, John Joseph Rosskopf

Open Access Theses & Dissertations

The family Nodaviridae is comprised of two genera: the alphanodaviruses that infect insects and the betanodaviruses, which have been isolated only from fish. Nodamura virus (NoV), the type species of the alphanodavirus genus, can lethally infect both insects (mosquitoes, honey bees, and wax moth larvae) and mammals (suckling mice and suckling hamsters). In addition, nodavirus RNAs can replicate in a wide variety of host cells, including those of mammalian, insect, plant, and yeast origin, when introduced by transfection. The nodavirus positive strand RNA genome is naturally segregated into two segments. RNA1 encodes the viral RNA-dependent RNA polymerase (RdRp) that replicates …

Geography Of Pestecide Exposure In The Lower Valley (El Paso County, Texas), Critina Solis Sanchez

Open Access Theses & Dissertations

Previous studies using GIS have been used to understand the demographics of particular areas of a city and the risks associated with living there. In the Lower Valley (Socorro, Clint, and San Elizario) area of El Paso County (TX), there has been a rapid growth in population. This increase in growth has led to this project about the types of pesticides used in farms and if they are creating possible health risks for people living in this area and which populations are most at risk. To determine population risk, I use existing GIS information, specifically a USDA aerial photograph taken …

Salt Separation Processes In Salt Cedar, Rudy Sookbirsingh

Open Access Theses & Dissertations

Salt cedar (genus Tamarix, Family Tamaricaceae) is an exotic, invasive plant in southwestern North America; it has a poor reputation as a heavy-consumer of water and a threat to the landscape and ecology of the region. This study focuses upon saltseparation mechanisms in the salt cedar species Tamarix ramossissima (Ledeb.) which were investigated with a variety of microscopic techniques. The results indicate that this species can produce different salt crystal aggregates morphologies from its vesiculated trichomes, and may use variable mechanisms for separating anions and cations of various salts. It was additionally found that salt crystals aggregates in this species …

Machiavellian Heroes Through The Prism Of Aristotle, Robert Acosta

Open Access Theses & Dissertations

This Thesis examines the relationship between the political philosophies of Machiavelli and Aristotle. It hypothesizes that Machiavelli incorporates three fundamental premises of Aristotle's political philosophy: 1. Politics is the master science 2. Man is a political animal 3. Aristotle's conception of human nature. In order to test the hypoThesis the Thesis utilizes case studies in the form of Machiavelli's four excellent men (Theseus, Romulus, Cyrus, and Moses) that are named explicitly in Chapter VI of The Prince . It is concluded that there is an element of Aristotle's fundamental political philosophy that is incorporated by Machiavelli. This relationship is often …

Rival Radical Feminists--Frances Willard And Ida B. Wells: The Rhetorical Slugfest Of Two Nineteenth-Century Queen Bees Over Lynching, Anita August

Open Access Theses & Dissertations

Rival Radical Feminists considers the role of gender and race as master status determining traits and examines them as influential social markers of identity and representation within a nineteenth-century feminist social movement (FSM)--the Woman's Christian Temperance Union (WCTU). Rival Radical Feminists examines how, within a FSM where gender issues understandably govern the political narrative that the philosophical core of the movement shifts into separate and competing spheres when gender issues intersect with racial prejudice? Specifically, Rival Radical Feminists argues that when both political actors are female, with one circumscribed politically by her gender, like Willard, and the other by both …

Science Versus Religion: Protestant Dominance And Cultural Discrimination In Public Schools, Melissa Marie Brock

Open Access Theses & Dissertations

This research offers a critical analysis of the current controversy concerning religion. The study explores the establishment of the public school system under Protestant dominance and use of this dominance to assert authority over minority groups. Legislation and court action have created a position of opposing policy; placing science as the opposite of religion, and Atheists and Christian activists are using curriculum as an ideological battle field. Currently, since 9/11, religious discrimination and hate crimes are on the rise. In order to investigate this controversy, this study explores literature, published surveys, and government documents. The American public school systems are …

Using The Taguchi Design And The Central Composite Design To Increase The Robustness Of A Process From Its Raw Material Variability, Alvaro Cuevas

Open Access Theses & Dissertations

This research presents an experimental study where four controllable and one noise factor are analyzed using the Taguchi approach and the Central Composite Design (CCD) in order to induce robustness in a process that is being designed to treat residual grease from small restaurants. Three response variables are analyzed separately to establish the parameters conditions to obtain the most robust process output. These suggested conditions are validated conducting confirmation runs that are compared statistically to the predictions from the Taguchi prediction model for the Taguchi approach, and the Response optimizer for the CCD. The accuracy of each method is measured …