Digital Commons Network^™

Helminth Genomics: The Implications For Human Health, Paul J. Brindley, Makedonka Mitreva, Elodie Ghedin, Sara Lustigman

Open Access Publications

No abstract provided.

Degradation Of Host Sphingomyelin Is Essential For Leishmania Virulence, Ou Zhang, Mattie C. Wilson, Wei Xu, Fong-Fu Hsu, John Turk, F. Matthew Kuhlmann, Yingwei Wang, Lynn Soong, Phillip Key, Stephen M. Beverley, Kai Zhang

Open Access Publications

No abstract provided.

Selection At A Single Locus Leads To Widespread Expansion Of Toxoplasma Gondii Lineages That Are Virulent In Mice, Asis Khan, Sonya Taylor, James W. Ajioka, Benjamin M. Rosenthal, L. David Sibley

Open Access Publications

No abstract provided.

A Role Of Scn9a In Human Epilepsies, As A Cause Of Febrile Seizures And As A Potential Modifier Of Dravet Syndrome, Nanda A. Singh, Chris Pappas, E. Jill Dahle, Lieve R. F. Claes, Timothy H. Pruess, Peter De Jonghe, Joel Thompson, Missy Dixon, Christina Gurnett, Andy Peiffer, H. Steve White, Francis Filloux, Mark F. Leppert

Open Access Publications

No abstract provided.

In Vitro Growth Environment Produces Lipidomic And Electron Transport Chain Abnormalities In Mitochondria From Nontumorigenic Astrocytes And Brain Tumours, Michael A. Kiebish, Xianlin Han, Hua Cheng, Thomas N. Seyfried

Open Access Publications

No abstract provided.

Bringing Kash Under The Sun: The Many Faces Of Nucleo-Cytoskeletal Connections, David Razafsky, Didier Hodzic

Open Access Publications

No abstract provided.

Evidence For High Bi-Allelic Expression Of Activating Ly49 Receptors, Arefeh Rouhi, C. B. Lai, Tammy P. Cheng, Fumio Takei, Wayne M. Yokoyama, Dixie L. Mager

Open Access Publications

No abstract provided.

Nematode.Net Update 2008: Improvements Enabling More Efficient Data Mining And Comparative Nematode Genomics, John Martin, Sahar Abubucker, Todd Wylie, Yong Yin, Zhengyuan Wang, Makedonka Mitreva

Open Access Publications

No abstract provided.

Key Role Of T Cell Defects In Age-Related Vulnerability To West Nile Virus, James D. Brien, Jennifer L. Uhrlaub, Alec Hirsch, Clayton A. Wiley, Janko Nikolich-Zugich

Open Access Publications

West Nile virus (WNV) infection causes a life-threatening meningoencephalitis that becomes increasingly more prevalent over the age of 50 and is 40-50x more prevalent in people over the age of 70, compared with adults under the age of 40. In a mouse model of age-related vulnerability to WNV, we demonstrate that death correlates with increased viral titers in the brain and that this loss of virus control with age was the result of defects in the CD4 and CD8 T cell response against WNV. Specific age-related defects in T cell responses against dominant WNV epitopes were detected at the level …

Trem-2 (Triggering Receptor Expressed On Myeloid Cells 2) Is A Phagocytic Receptor For Bacteria, Elsa-Noah N'Diaye, Catherine S. Branda, Steven S. Branda, Lisette Nevarez, Marco Colonna, Clifford Lowell, Jessica A. Hamerman, William E. Seaman

Open Access Publications

Phagocytosis, which is essential for the immune response to pathogens, is initiated by specific interactions between pathogens and cell surface receptors expressed by phagocytes. This study identifies triggering receptor expressed on myeloid cells 2 (TREM-2) and its signaling counterpart DAP12 as a molecular complex that promotes phagocytosis of bacteria. Expression of TREM-2-DAP12 enables nonphagocytic Chinese hamster ovary cells to internalize bacteria. This function depends on actin cytoskeleton dynamics and the activity of the small guanosine triphosphatases Rac and Cdc42. Internalization also requires src kinase activity and tyrosine phosphorylation. In bone marrow-derived macrophages, phagocytosis is decreased in the absence of DAP12 …

Cdc42 Antagonizes Rho1 Activity At Adherens Junctions To Limit Epithelial Cell Apical Tension, Stephen J. Warner, Gregory D. Longmore

Open Access Publications

In epithelia, cells are arranged in an orderly pattern with a defined orientation and shape. Cadherin containing apical adherens junctions (AJs) and the associated actomyosin cytoskeleton likely contribute to epithelial cell shape by providing apical tension. The Rho guanosine triphosphatases are well known regulators of cell junction formation, maintenance, and function. Specifically, Rho promotes actomyosin activity and cell contractility; however, what controls and localizes this Rho activity as epithelia remodel is unresolved. Using mosaic clonal analysis in the Drosophila melanogaster pupal eye, we find that Cdc42 is critical for limiting apical cell tension by antagonizing Rho activity at AJs. Cdc42 …

Impact Speed Does Not Determine Severity Of Spinal Cord Injury In Mice With Fixed Impact Displacement, Joong Hee Kim, Tsang-Wei Tu, Philip V. Bayly, Sheng-Kwei Song

Open Access Publications

No abstract provided.

A Proposal For A Coordinated Effort For The Determination Of Brainwide Neuroanatomical Connectivity In Model Organisms At A Mesoscopic Scale, Joseph L. Price, David C. Van Essen, Et Al.

Open Access Publications

No abstract provided.

Identification Of Atg5-Dependent Transcriptional Changes And Increases In Mitochondrial Mass In Atg5-Deficient T Lymphocytes, Linda M. Stephenson, Brian C. Miller, Aylwin Ng, Jason Eisenberg, Zijiang Zhao, Ken Cadwell, Daniel B. Graham, Noboru N. Mizushima, Ramnik Xavier, Herbert W. Virgin, Wojciech Swat

Open Access Publications

No abstract provided.

Skin-Derived Tslp Triggers Progression From Epidermal- Barrier Defects To Asthma, Shadmehr Demehri, Mitsuru Morimoto, Michael J. Holtzman, Raphael Kopan

Open Access Publications

No abstract provided.

How Do Animal Mitochondria Tolerate Water Stress?, Michael Menze, Steven Hand

Faculty and Staff Scholarship

The vast majority of animal species do not tolerate severe water stress, but the encysted embryo of the brine shrimp Artemia franciscana is an exceptionally useful organism to investigate physiological mechanisms for enduring extreme environmental insults. Any substantial reduction in cellular water poses a threat to survival. Nevertheless anhydrobiotic animals survive virtually complete loss of cellular water. The mechanisms that govern “life without water” (anhydrobiosis) are still not well understood. With certain exceptions, it seems that a recurring strategy for tolerating severe water loss involves the accumulation of both low molecular weight solutes (e.g. trehalose or other polyol) and highly …

Do Radiation-Induced Bystander Effects Correlate To The Intrinsic Radiosensitivity Of Individuals And Have Clinical Significance?, Orla Howe, Jacintha O'Sullivan, Blathnaid Nolan, Joe Vaughan, Sheeona Gorman, Colin Clarke, Brendan Mcclean, Fiona M. Lyng

Research Outputs: 2025-Present

It is well known that patients can vary in their normal tissue response to radiotherapy, and this can be problematic. As a result, radiobiologists have been using in vitro models to assess variation in response and elucidate the genetic determinants of this variation. However, the clinical relevance of these models is currently unknown. In this study, blood samples from healthy controls (n 20) and colorectal carcinoma patients (n 60) were cultured in vitro to assess two radiobiological end points in parallel: intrinsic radiosensitivity assayed by chromosomal aberrations (G2 scores) and radiation-induced bystander effects assayed by viability testing. Increased intrinsic radiosensitivity …

Kevan Francis V. The State Of Utah : Brief Of Appellant, Utah Supreme Court

Utah Supreme Court Briefs (2000–2020)

BRIEF OF PLAINTIFFS-APPELLANTS No. 20090256 Oral argument requested Appeal from the Judgment of the Fourth Judicial District Court, Utah County The Honorable Gary D. Stott, District Court Judge, Presiding District Court Case No. 080401029

A-T Asphalt Paving Inc V. Braffits Creek Estates, Inc., Omnia Development, Inc., Crescent Properties, Llc, Anb Financial N.A., Kennedy Funding, Inc., Schwab Sales, Llc, And John Does 1-5 : Brief Of Appellee, Utah Supreme Court

Utah Supreme Court Briefs (2000–2020)

Appeal from the Fifth District Court, Iron County Honorable John J. Walton, District Judge

Influence Of Vitamin E On Innate And Humoral Components Of The Immune System Of Pregnant Ewes And Their Lambs, Saritha Anugu

Open Access Master's Theses

The effect of parenteral administration of vitamin E during the pregnancy and lactation period of ewes and their lambs on innate and humoral components of the immune system was investigated in a 3 part study. 1.) Pregnant/Lactating ewes: Fifteen pregnant Dorset sheep were supplemented with vitamin E (30 IU d-α-tocopherol /Kg body weight (BW), E, n=l0) or placebo (emulsified base, P, n=5) from the last trimester of gestation through weaning. Blood and colostrum were analyzed every 2 wk for α-tocopherol, selenium, glutathione peroxidase activity, lysozyme activity, total IgG and IgG antibodies against tetanus toxoid. 2.) Lambs (0-8wks): Lambs born to …

"Say On Pay": Cautionary Notes On The U.K. Experience And The Case For Shareholder Opt-In, Jeffrey N. Gordon

Faculty Scholarship

Shareholder and public dissatisfaction with executive compensation has led to calls for an annual shareholder advisory vote on firms’ compensation practices and policies, so-called “say on pay.” Proposed federal legislation would mandate “say on pay” generally for U.S. public companies. This Article assesses the case for such a mandatory federal rule in light of the U.K. experience with a similar regime adopted in 2002. The best argument for a mandatory rule is that it would destabilize pay practices that have produced excessive compensation and that would not yield to firm-by-firm pressure. This has not been the U.K. experience; pay continues …

Tall Fescue (Lolium Arundinaceum (Schreb.) S.J. Darbyshire) Cultivar Performance In The Central California Coastal Region, Seril Shimizu

Master's Theses

Tall fescue (Lolium arundinaceum (Schreb.) S.J. Darbyshire) has long been known as a turfgrass that utilizes lower levels of inputs than most other cool season turfgrasses (Turgeon, 2005). In recent years, turf breeders have been selecting for cultivars of tall fescue that have finer leaf textures. As the use of tall fescue expands, consumers want to now what cultivar to use and how to manage it. This study looked at 18 tall fescue cultivars grown at two mowing heights of five and 10 centimeters and evaluated plant quality based on stand density, leaf texture, and genetic plant color. In the …

The Effects Of Aging On Muscle Loss And Nuclear Factor Kappa-B Levels In Rats Fed A Diet Containing Suboptimal Leucine Levels, Corinne Rose Kohlen

Master's Theses

Loss of muscle due to aging is often associated with significant detrimental effects. Therefore, it is crucial to understand signaling molecules that may trigger the muscle loss or prevent the process. The transcription factor, Nuclear Factor Kappa-B (NF-κB), is associated with both catabolic and anabolic pathways of muscle metabolism and may be involved in age-related muscle loss. Leucine is an essential amino acid that is required for both protein synthesis and intracellular signaling pathways that regulate protein synthesis and degradation. The current study examined muscle NF-kB levels in male Sprague-Dawley rats, aged 6 (adult) and 21 months (old) fed a …

A Unified Resource Platform For The Rapid Development Of Scalable Web Applications, Russell Palmiter

Master's Theses

This thesis presents Web Utility Kit (WUT): a platform that helps to simplify the process of creating modern web applications. It addresses the need to simplify the web development process through the creation of a hosted service that provides access to a unified set of resources. The resources are made available through a variety of protocols and formats to help simplify their consumption. It also provides a uniform model across all of its resources making multi-resource development an easier and more familiar task. WUT saves the time and cost associated with deployment, maintenance, and hosting of the hardware and software …

Burden Of Proof In Environmental Disputes In The Wto: Legal Aspects, Henrik Horn, Petros C. Mavroidis

Faculty Scholarship

This paper discusses allocation of burden of proof in environmental disputes in the WTO system. Besides laying down the natural principles that (i) the complainant carries the burden to (ii) make a prima facie case that its claim holds, WTO adjudicating bodies have said little of more general nature. The paper therefore examines the case law of relevance to environmental policies, to establish the rules concerning burden of proof that are likely to be applied in such disputes. Evaluating this case law, the paper makes two observations,: First, in cases submitted under the GATTWTO, adjudicating bodies have committed errors regarding …

The Writer-Of-Violence-As-School-Shooter Stereotype: How Columbine, Virginia Tech, And Public Fear Make Writing Violence Dangerous For Students, Matt Foy

Dissertations and Theses @ UNI

In a period when students, parents, teachers and administrators are bombarded with images related to school shooting attacks, efforts to prevent such attacks in the future and to identify students who pose a threat to school safety have intensified. These efforts to fight the seemingly mounting wave of school violence have contributed to the rise of stereotypes of what a school shooter looks, talks, and acts like. This study examines one of these stereotypes: the writer-of-violence-as-school-shooter stereotype. This stereotype, which gained attention after 1990s rampages such as those at Heath High School (Kentucky) and Columbine High School and became highly …

Construction And Sequence Sampling Of Deep-Coverage Large-Insect Bac Libraries For Three Model Lepidopteran Species, Chengcang Wu, Dina Proestou, Dorothy Carter, Erica Nicholson, Filippe Santos, Shaying Zhao, Hong-Bin Zhang, Marian R. Goldsmith

Biological Sciences Faculty Publications

Background: Manduca sexta, Heliothis virescens, and Heliconius erato represent three widely-used insect model species for genomic and fundamental studies in Lepidoptera. Large-insert BAC libraries of these insects are critical resources for many molecular studies, including physical mapping and genome sequencing, but not available to date.

Results: We report the construction and characterization of six large-insert BAC libraries for the three species and sampling sequence analysis of the genomes. The six BAC libraries were constructed with two restriction enzymes, two libraries for each species, and each has an average clone insert size ranging from 152–175 kb. We estimated that …

Political Control Of Federal Prosecutions: Looking Back And Looking Forward, Daniel C. Richman

Faculty Scholarship

This Essay explores the mechanisms of control over federal criminal enforcement that the administration and Congress used or failed to use during George W. Bush's presidency. It gives particular attention to Congress, not because legislators played a dominant role, but because they generally chose to play such a subordinate role. My fear is that the media focus on management inadequacies or abuses within the Justice Department during the Bush administration might lead policymakers and observers to overlook the hard questions that remain about how the federal criminal bureaucracy should be structured and guided during a period of rapidly shifting priorities …

The Effect Of Zinc On L-Leucine Transport In Lobster Intestine, Anna Lynn Mullins

UNF Graduate Theses and Dissertations

The current study is a characterization of L-leucine absorption across the American lobster intestine in order to determine the likelihood of a bis-complex formation with zinc ([Leu]-Zn-[Leu]) as a possible substrate for PEPT1. This study required isolated lobster intestines to be mounted in a perfusion chamber in order to quantify 3H-L-leucine transport. It was hypothesized that an apical dipeptide transporter, PEPT1, was responsible for uptake of L-leucine via formation of a bis-complex with zinc ([Leu]-Zn-[Leu]) in the form of molecular mimicry, where the bis-complex mimics the normal dipeptide substrate of the proposed carrier system. It was found that L-leucine transport …

Arabidopsis Thaliana Glx2-1 Contains A Dinuclear Metal Binding Site, But Is Not A Glyoxalase 2, Pattraranee Limphong, Michael W. Crowder, Brian Bennett, Christopher A. Makaroff

Physics Faculty Research and Publications

In an effort to probe the structure and function of a predicted mitochondrial glyoxalase 2, GLX2-1, from Arabidopsis thaliana, GLX2-1 was cloned, overexpressed, purified and characterized using metal analyses, kinetics, and UV–visible, EPR, and ¹H-NMR spectroscopies. The purified enzyme was purple and contained substoichiometric amounts of iron and zinc; however, metal-binding studies reveal that GLX2-1 can bind nearly two equivalents of either iron or zinc and that the most stable analogue of GLX2-1 is the iron-containing form. UV–visible spectra of the purified enzyme suggest the presence of Fe(II) in the protein, but the Fe(II) can be oxidized over …