Digital Commons Network^™

Economic Impact Of Bovine Tuberculosis On Minnesota’S Cattle And Beef Sector, Brian Buhr, Kyle Mckeever, Kenji Adachi

Michigan Bovine Tuberculosis Bibliography and Database

In September 2005, the first case of bovine tuberculosis (BTB) in 34 years was discovered in Minnesota and led to a loss of Minnesota’s bovine TB free status. In October 2007 and January and February 2008, four additional cattle herds and deer infected with BTB were discovered in Roseau and Beltrami counties and Minnesota’s BTB status was changed from “modified accredited advanced” to “modified accredited” in April 2008. Each of these designations has increased regulatory requirements (and costs) for animal testing, record keeping, eradication practices, slaughtering and shipments of cattle within state and across state lines.

Humoral Immune Responses Of White-Tailed Deer (Odocoileus Virginianus) To Mycobacterium Bovis Bcg Vaccination And Experimental Challenge With M. Bovis, P. Nol, K. P. Lyashchenko, R. Greenwald, J. Esfandiari, W. R. Waters, M. V. Palmer, B. J. Nonnecke, T. J. Keefe, T. C. Thacker, J. C. Rhyan, F. E. Aldwell, M. D. Salman

Michigan Bovine Tuberculosis Bibliography and Database

Monitoring of the kinetics of production of serum antibodies to multiple mycobacterial antigens can be useful as a diagnostic tool for the detection of Mycobacterium bovis infection as well as for the characterization of disease progression and the efficacy of intervention strategies in several species. The humoral immune responses to multiple M. bovis antigens by white-tailed deer vaccinated with BCG orally via a lipid-formulated bait (n = 5), orally in liquid form (n = 5), and subcutaneously (n = 6) were evaluated over time after vaccination and after experimental challenge with virulent M. bovis and were compared to the responses …

Evaluation Of Blood Assays For Detection Of Mycobacterium Bovis In White-Tailed Deer (Odocoileus Virginianus) In Michigan, Daniel J. O’Brien, Stephen M. Schmitt, Konstantin P. Lyashchenko, W. Ray Waters, Dale E. Berry, Mitchell V. Palmer, Jim Mcnair, Rena Greenwald, Javan Esfandiari, Melinda K. Cosgrove

Michigan Bovine Tuberculosis Bibliography and Database

Surveillance and control activities related to bovine tuberculosis (TB) in free-ranging, Michigan white-tailed deer (Odocoileus virginianus) have been underway for over a decade, with significant progress. However, foci of higher TB prevalence on private lands and limited agency ability to eliminate them using broad control strategies have led to development and trial of new control strategies, such as live trapping, testing, and culling or release. Such strategies require a prompt, accurate live animal test, which has thus far been lacking. We report here the ability of seven candidate blood assays to determine the TB infection status of Michigan …

Tuberculosis: A Re-Emerging Disease In Animals And Humans, Charles O. Thoen, Philip A. Lobue, Donald A. Enarson, John B. Kaneene, Isabel N. De Kantor

Michigan Bovine Tuberculosis Bibliography and Database

Tuberculosis continues to be an important disease both in humans and animals. It causes morbidity, mortality, and economic loss worldwide. The occurrence of Mycobacterium bovis disease in humans, domesticated and wild animals confirms the relevance of this zoonosis. M. bovis in humans continues to be reported in industrialized countries and in immigrants from regions of the world where tuberculosis in cattle is endemic. The real incidence of M. bovis in humans in developing countries continues to be roughly underestimated due to the scarcity of appropriate laboratory facilities to isolate and to differentiate M. bovis strains. In Latin America, less than …

The Leading Object: August 2009, John C. Owens

Leading Object

Perspectives; Undergraduate students from Chinese university conduct research at UNL; Water Center change underway; Waldren retires after 35 years with UNL; Hansen agricultural ties important in his legislative service; Dairy Store’s goal is to serve its customers well; UNL’s organic certified acres to provide valuable insight to producers; Extension plan helps keep lakes free of toxic algae; Henneman receives nutrition award; Rasby receives beef industry award

The Leading Object: July 2009, John C. Owens

Leading Object

Perspectives; Costa Rican study trip helps insect science students learn biodiversity; Berger named head of Department of Animal Science; Milligan stays close to Nebraska agricultural roots; Awards; Ag Builders group gets insights into workings of IANR efforts; Deaths; Hostetler, Denning receive OEA honors

The Leading Object: March 2009, John C. Owens

Leading Object

Perspectives; Agreement expected to expand food industry in Nebraska, India; Holling Family Awards presented to faculty; Love of animals leads to career in veterinary medicine; CASNR Week planned April 17-25; Meat judging team places high in competitions; UNL selected to participate in USDA pilot biotech project; Dewey sees storm chasing as both educational and a service; Chesnin, former agronomy professor, dies

The Leading Object: November/December 2009, John C. Owens

Leading Object

Perspectives; EARTH University students gain extension experience in Grand Island; NCTA accreditation continues into next decade; 91-year-old alumus deciding next chapter in life; Whitman rancher says extension enables profitability; Commission approves school name; Glewen receives Omtvedt Innovation Award; Awards; Deaths

The Leading Object: October 2009, John C. Owens

Leading Object

Perspectives; IANR faculty helps develop forensic science in Lagos; Agronomy professor emeritus dies; Mazour works to produce food to feed world’s population; Food ingredient with soybeans, tallow shown to lower cholesterol; Baenziger receives 2009 Omtvedt Innovation Award; Cunningham steps down as ARD dean and director; Agribusiness club gives awards

The Leading Object: September 2009, John C. Owens

Leading Object

Perspectives; Awareness and demand for local foods keep growing; CASNR alumni enjoy reunion, auction; Bosshamer’s interest in beef started at an early age; Construction progress, new opportunities greet returning students; FPC holding food workshops for audiences around the world; Dvorak named interim Water Center director; CASNR grad gets ACT award; Sutton named Fellow of Society of Landscape Architects; Cunningham receives KUDO

A New Holcocera Clemens From Guatemala And Redescription Of H. Iceryaeella (Riley) From The United States (Lepidoptera: Coleophoridae: Blastobasinae: Holcocerini): Two Congeners With Incidental Preference For Avocado, David Adamski, Mark Hoddle

USDA Systematic Entomology Laboratory

Two species of Holcocera Clemens (Lepidoptera: Coleophoridae: Blastobasinae: Holcocerini) are known to feed on Persea americana Mill. (Lauraceae), but their frequency of infestation appears low. One species, Holcocera plagatola, n. sp., from Guatemala, is described herein. Holcocera iceryaeella (Riley) is known only from California and has been recorded on many different host plants; it is also known to be a predator of immature Hemiptera and scale insects. Photographs of the imagos of both Holcocera species are included, in addition to illustrations of the male and female genitalia. We comment on host preferences for both species.

Paris Main Street, Josh Hollis

Historic Preservation Final Projects

No abstract provided.

Genetic And Evolutionary Considerations In Fishery Management: Research Needs For The Future, Robin Waples, Kerry A. Naish

United States Department of Commerce: Staff Publications

Genetic methods have become indispensable for sound fishery management and will become even more so in the twenty-first century. Selectively neutral genetic markers are widely used in stock identification, mixed-stock fishery analysis, monitoring levels of genetic diversity within populations and levels of connectivity among populations, and for a range of other applications. We expect that future research will continue to provide incremental improvements in the number and type of genetic markers available, as well as in the methods for data analysis and the necessary computational resources. Topics that will merit special consideration include: (1) developing a better understanding of the …

Seasonality, Estrous Cycle Characterization, Estrus Synchronization, Semen Cryopreservation, And Artificial Insemination In The Pacific White-Sided Dolphin (Lagenorhynchus Obliquidens), T. R. Robeck, K. J. Steinman, M. Greenwell, K. Ramirez, W. Van Bonn, M. Toshioka, E. Katsumata, L. Dalton, S. Osborn, J. K. O'Brien

United States Department of Commerce: Staff Publications

The reproductive physiology of the Pacific white-sided dolphin, Lagenorhynchus obliquidens, was characterized to facilitate the development of artificial insemination (AI) using cryopreserved spermatozoa. Specific objectives were to: 1) describe reproductive seasonality of the Pacific white sided dolphins; 2) describe urinary LH and ovarian steroid metabolites during the estrous cycle; 3) correlate LH and ovarian steroidal metabolite patterns to ultrasound-monitored follicular growth and ovulation; and 4) assess the efficacy of synchronizing estrus, sperm collection/cryopreservation, and intrauterine insemination. Ovulations (64%, n=37) and conceptions (83%, n=18) occurred from August to October. Peak mean serum testosterone (24 ng/ml), cross-sectional testicular area (41.6 cm …

Tomato Bushy Stunt Virus (Tbsv), A Versatile Platform For Polyvalent Display Of Antigenic Epitopes And Vaccine Design, Shantanu Kumar, Wendy Ochoa, Pratik Singh, Catherine Hsu, Anette Schneemann, Marianne Manchester, Mark Olson, Vijay Reddy

United States Army: Publications

Viruses-like particles (VLPs) are frequently being used as platforms for polyvalent display of foreign epitopes of interest on their capsid surface to improve their presentation enhancing the antigenicity and host immune response. In the present study, we used the VLPs of Tomato bushy stunt virus (TBSV), an icosahedral plant virus, as a platform to display 180 copies of 16 amino acid epitopes of ricin toxin fused to the C-terminal end of a modified TBSV capsid protein (NΔ52). Expression of the chimeric recombinant protein in insect cells resulted in spontaneous assembly of VLPs displaying the ricin epitope. Cryo-electron microscopy and image …

Proteomic Analyses Of Native Brain Kv4.2 Channel Complexes, Celine Marionneau, Richard D. Leduc, Henry W. Rohrs, Andrew J. Link, R. Reid Townsend, Jeanne M. Nerbonne

Open Access Publications

No abstract provided.

High-Resolution Sonography Of The Normal Extrapelvic Vas Deferens, William D. Middleton, Nirvikar Dahiya, Cathy K. Naughton, Sharlene A. Teefey, Cary A. Siegel

Open Access Publications

No abstract provided.

Phenotypic And Gene Expression Changes Among Clonal Type I Strains Of Toxoplasma Gondit, Asis Khan, Michael S. Behnke, Ildiko R. Dunay, Michael W. White, L. David Sibley

Open Access Publications

No abstract provided.

Mechanoregulation Of Proliferation, Xiaogang Jiang, Paul F. Austin, Robert A. Niederhoff, Scott R. Manson, Jacob J. Riehm, Brian L. Cook, Gina Pengue, Kanchan Chitaley, Keiko Nakayama, Keiichi I. Nakayama, Steven J. Weintraub

Open Access Publications

No abstract provided.

Mrn Complex Function In The Repair Of Chromosomal Rag-Mediated Dna Double-Strand Breaks, Beth A. Helmink, Andrea L. Bredemeyer, Baeck-Seung Lee, Ching-Yu Huang, Girdhar G. Sharma, Laura M. Walker, Jeffrey J. Bednarski, Wan-Ling Lee, Tej K. Pandita, Craig H. Bassing, Barry P. Sleckman

Open Access Publications

The Mre11-Rad50-Nbs1 (MRN) complex functions in the repair of DNA double-strand breaks (DSBs) by homologous recombination (HR) at postreplicative stages of the cell cycle. During HR, the MRN complex functions directly in the repair of DNA DSBs and in the initiation of DSB responses through activation of the ataxia telangiectasia-mutated (ATM) serine-threonine kinase. Whether MRN functions in DNA damage responses before DNA replication in G0/G1 phase cells has been less clear. In developing G1-phase lymphocytes, DNA DSBs are generated by the Rag endonuclease and repaired during the assembly of antigen receptor genes by the process of V(D)J recombination. Mice and …

Wolf, Canis Lupus, Visits To White-Tailed Deer, Odocoileus Virginianus, Summer Ranges: Optimal Foraging?, Dominic J. Demma, L. David Mech

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

We tested whether Wolf (Canis lupus) visits to individual female White-tailed Deer (Odocoileus virginianus) summer ranges during 2003 and 2004 in northeastern Minnesota were in accord with optimal-foraging theory. Using GPS collars with 10- to 30-minute location attempts on four Wolves and five female deer, plus eleven VHF-collared female deer in the Wolves’ territory, provided new insights into the frequency of Wolf visits to summer ranges of female deer. Wolves made a mean 0.055 visits/day to summer ranges of deer three years and older, significantly more than their 0.032 mean visits/day to ranges of two-year-old deer, …

White-Nose Syndrome Threatens The Survival Of Hibernating Bats In North America, U.S. Geological Survey

United States Geological Survey: Publications

During the winter of 2006/2007, an affliction of unknown origin dubbed “White-Nose Syndrome” (WNS) began devastating colonies of hibernating bats in a small area around Albany, New York. Colonies of hibernating bats were reduced 81-97% at the affected caves and mines that were surveyed. Since then, White-Nose Syndrome has been detected more than 700 kilometers (450 mi) away from the original site, and has infected bats in eight surrounding states. Most species of bats that hibernate in the region are now known to be affected and little brown bats (Myotis lucifugus), northern long-eared bats (M. septentrionalis), …

Ecological Requirements For Pallid Sturgeon Reproduction And Recruitment In The Lower Missouri River: A Research Synthesis 2005–08, Aaron J. Delonay, Robert B. Jacobson, Diana M. Papoulias, Darin G. Simpkins, Mark L. Wildhaber, Joanna M. Reuter, Tom W. Bonnot, Kimberly A. Chojnacki, Carl E. Korschgen, Gerald E. Mestl, Michael J. Mac

United States Geological Survey: Publications

This report provides a synthesis of results obtained between 2005 and 2008 from the Comprehensive Sturgeon Research Program, an interagency collaboration between the U.S. Geological Survey, Nebraska Game and Parks Commission, U.S. Fish and Wildlife Service, and the U.S. Army Corps of Engineers’ Missouri River Recovery―Integrated Science Program. The goal of the Comprehensive Sturgeon Research Program is to improve fundamental understanding of reproductive ecology of the pallid sturgeon with the intent that improved understanding will inform river and species management decisions. Specific objectives include:

• Determining movement, habitat-use, and reproductive behavior of pallid sturgeon;

• Understanding reproductive physiology of pallid …

Chapter 2: Long-Term Research On Wolves In The Superior National Forest, L. David Mech

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

The seeds for the blossoming of the wolf (Canis lupus) population throughout the upper Midwest were embodied in a long line of wolves that had persisted in the central part of the Superior National Forest (SNF) of northeastern Minnesota, probably since the retreat of the last glaciers. This line of wolves had withstood not only the various natural environmental factors that had shaped them through their evolution but also the logging, fires, market hunting of prey animals, and even the bounties, aerial hunting, and poisoning that had exterminated their ancestors and their dispersed offspring only a few wolf …

Proteomic Comparison Of Entamoeba Histolytica And Entamoeba Dispar And The Role Of E. Histolytica Alcohol Dehydrogenase 3 In Virulence, Paul H. Davis, Minghe Chen, Xiaochun Zhang, C. Graham Clark, R. Reid Townsend, Samuel L. Stanley Jr.

Open Access Publications

No abstract provided.

Effects Of Mining-Derived Metals On Riffle-Dwelling Benthic Fishes In Southeast Missouri, Usa, A. L. Allert, J. F. Fairchild, C. J. Schmitt, J. M. Besser, W. G. Brumbaugh, S. J. Olson

United States Geological Survey: Staff Publications

We studied the ecological effects of mining-derived metals on riffle-dwelling benthic fishes at 16 sites in the Viburnum Trend lead–zinc mining district of southeast Missouri. Fish community attributes were compared to watershed features and to physical and chemical variables including metal concentrations in sediment pore water and fish. Ozark sculpin (Cottus hypselurus), rainbow darter (Etheostoma caeruleum), Ozark madtom (Noturus albater), and banded sculpin (Cottus carolinae) were the most abundant fishes collected. Species richness and density of riffle-dwelling benthic fishes were negatively correlated with metal concentrations in pore water and in fish. Sculpin …

Persistence Of Organochlorine Chemical Residues In Fish From The Tombigbee River (Alabama, Usa): Continuing Risk To Wildlife From A Former Ddt Manufacturing Facility, Jo Ellen Hinck, Ross J. Norstrom, Carl E. Orazio, Christopher J. Schmitt, Donald E. Tillitt

United States Geological Survey: Staff Publications

Organochlorine pesticide and total polychlorinated biphenyl (PCB) concentrations were measured in largemouth bass from the Tombigbee River near a former DDT manufacturing facility at McIntosh, Alabama. Evaluation of mean p,p'- and o,p'-DDT isomer concentrations and o,p'- versus p,p'-isomer proportions in McIntosh bass indicated that DDT is moving off site from the facility and into the Tombigbee River. Concentrations of p,p'-DDT isomers in McIntosh bass remained unchanged from 1974 to 2004 and were four times greater than contemporary concentrations from a national program. Total DDT in McIntosh bass exceeded dietary effect concentrations developed for bald eagle and osprey. Hexachlorobenzene, PCBs, and …

Toxicity Of Polybrominated Diphenyl Ethers (De-71) In Chicken (Gallus Gallus), Mallard (Anas Platyrhynchos), And American Kestrel (Falco Sparverius) Embryos And Hatchlings, Moira A. Mckernan, Barnett A. Rattner, Robert C. Hales, Mary Ann Ottinger

United States Geological Survey: Staff Publications

Embryonic survival, pipping and hatching success, and sublethal biochemical, endocrine, and histological endpoints were examined in hatchling chickens (Gallus gallus), mallards (Anas platyrhynchos), and American kestrels (Falco sparverius) following air cell administration of a pentabrominated diphenyl ether (penta-BDE; DE-71) mixture (0.01–20 µg/g egg) or polychlorinated biphenyl (PCB) congener 126 (3,3’,4,4’,5-pentachlorobiphenyl; 0.002 µg/g egg). The penta-BDE decreased pipping and hatching success at concentrations of 10 and 20 µg/g egg in kestrels but had no effect on survival endpoints in chickens or mallards. Sublethal effects in hatchling chickens included ethoxyresorufin-O-dealkylase (EROD) induction and histological …

Characterization Of Electrochemically Active Bacteria Utilizing A High-Throughput Voltage-Based Screening Assay, Justin C. Biffinger, Meghann Ribbens, Bradley Ringeisen, Jeremy Pietron, Steven Finkel, Kenneth Nealson

United States Navy: Publications

Metal reduction assays are traditionally used to select and characterize electrochemically active bacteria (EAB) for use in microbial fuel cells (MFCs). However, correlating the ability of a microbe to generate current from an MFC to the reduction of metal oxides has not been definitively established in the literature. As these metal reduction assays may not be generally reliable, here we describe a four- to nine-well prototype high throughput voltage-based screening assay (VBSA) designed using MFC engineering principles and a universal cathode. Bacterial growth curves for Shewanella oneidensis strains DSP10 and MR-1 were generated directly from changes in open circuit voltage …

Swine Immunity And Genetic Resistance To Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Infection, Joan K. Lunney, Derek Petry, Rodger Johnson, Daniel Kuhar, Ramon Molina, Jane Christopher-Hennings, Jeffrey Zimmerman, R. R. R. Rowland

School of Veterinary and Biomedical Sciences: Faculty Publications

Current vaccines are only partially effective against porcine reproductive and respiratory syndrome (PRRS) virus infection because they elicit a weak immune response that is not fully protective. PRRS is the most economically significant disease facing the swine industry today, costing U.S. pork producers at least $560 million annually. Despite substantial research efforts the exact components of a protective anti-PRRSV immune response are still not known, thus we are testing alternate approaches to evaluate immunity and genetic resistance to PRRSV. We used host genomics to compare different lines of pigs and look for factors that correlated with PRRSV resistance/ susceptibility. Viremia, …