Digital Commons Network^™

Researching African Women And Gender Studies: New Social Science Perspectives, Akosua Adomako Ampofo, Josephine Beoku-Betts, Mary J. Osirim

Sociology Faculty Research and Scholarship

Research on African women and gender studies has grown substantially to a position where African-centered gender theories and praxis contribute to theorizing on global feminist scholarship. Africanist scholars in this field have explored new areas such as transnational and multiracial feminisms, both of which address the complex and interlocking conditions that impact women's lives and produce oppression, opportunity and privilege. In addition, emergent African-centered research on women and gender explores those critical areas of research frequently addressed in the global North which have historically been ignored or marginalized in the African context such as family, work, social and political movements, …

Children's Beliefs And Family Law, Margaret F. Brinig

Journal Articles

In a recent series of opinions authored by Justice Stevens, the Court has recognized that children may have independent religious rights, and that these may be in conflict with their parents'. The questions for this piece are whether considering children's rights independently is a good thing whether it is warranted by children's actual religious preferences and whether children's religious activities actually do anything measurable for the children.

I do not advocate that the Supreme Court become more involved with family law than it has been since the substantive due process days of Meyer and Pierce. I am also not one …

Utep Magazine: The University Of Texas At El Paso, Utep University Communications

UTEP Magazine

To the Ends of the Earth UTEP at The Poles

Microbial Community Structure Dynamics In Ohio River Sediments During Reductive Dechlorination Of Pcbs, Andres Enrique Nunez

University of Kentucky Doctoral Dissertations

The entire stretch of the Ohio River is under fish consumption advisories due to contamination with polychlorinated biphenyls (PCBs). In this study, natural attenuation and biostimulation of PCBs and microbial communities responsible for PCB transformations were investigated in Ohio River sediments.

Natural attenuation of PCBs was negligible in sediments, which was likely attributed to low temperature conditions during most of the year, as well as low amounts of available nitrogen, phosphorus, and organic carbon. Moreover, surface sediments were relatively oxidized, as indicated by the prevalence of aerobic bacteria such as beta- Proteobacteria, alpha-Proteobacteria, Sphingobacteria, and Nitrospira in 16S rRNA sediment …

Valuations For Spike Train Prediction, Vladimir Itskov, Carina Curto, Kenneth D. Harris

Department of Mathematics: Faculty Publications

The ultimate product of an electrophysiology experiment is often a decision on which biological hypothesis or model best explains the observed data. We outline a paradigm designed for comparison of different models, which we refer to as spike train prediction. A key ingredient of this paradigm is a prediction quality valuation that estimates how close a predicted conditional intensity function is to an actual observed spike train. Although a valuation based on log likelihood (L) is most natural, it has various complications in this context. We propose that a quadratic valuation (Q) can be used as an alternative to L. …

Getting Past Conflict Resolution: A Complexity View Of Conflict, Leticia Andrade, Donde Ashmos Plowman, Dennis Duchon

Department of Management: Faculty Publications

The traditional view of conflict, as a problematic condition always requiring reduction or elimination and whose conditions or outcomes can be predicted, is incompatible with a complex adaptive systems view of organizations. Thus, conventional approaches to reducing conflict are often futile because the fundamental properties of complex adaptive systems are the source of much organizational ‘conflict.’ In this paper we offer an alternative view of conflict as pattern fluctuations in complex adaptive systems. Rather than needing reduction or elimination, conflict is the fuel that drives system growth and enables learning and adaptive behaviors, making innovation possible. Instead of focusing on …

Canine Vector-Borne Diseases In Brazil [Review], Filipe Dantas-Torres

Harold W. Manter Laboratory of Parasitology: Library Materials

Canine vector-borne diseases (CVBDs) are highly prevalent in Brazil and represent a challenge to veterinarians and public health workers, since some diseases are of great zoonotic potential. Dogs are affected by many protozoa (e.g. Babesia vogeli, Leishmania infantum, and Trypanosoma cruzi), bacteria (e.g. Anaplasma platys and Ehrlichia canis), and helminths (e.g. Dirofilaria immitis and Dipylidium caninum) that are transmitted by a diverse range of arthropod vectors, including ticks, fleas, lice, triatomines, mosquitoes, tabanids, and phlebotomine sand flies. This article focuses on several aspects (etiology, transmission, distribution, prevalence, risk factors, diagnosis, control, prevention, and public health …

Comparison And Validation Of Methods To Quantify Cry1ab Toxin From Bacillus Thuringiensis For Standardization Of Insect Bioassays, Andre L. B. Crespo, Terrence A. Spencer, Emily Nekl, Marianne Pusztai-Carey, William J. Moar, Blair D. Siegfried

Department of Entomology: Faculty Publications

Standardization of toxin preparations derived from Bacillus thuringiensis (Berliner) used in laboratory bioassays is critical for accurately assessing possible changes in the susceptibility of field populations of target pests. Different methods were evaluated to quantify Cry1Ab, the toxin expressed by 80% of the commercially available transgenic maize that targets the European corn borer, Ostrinia nubilalis (Hubner). We compared three methods of quantification on three different toxin preparations from independent sources: enzyme-linked immunosorbent assay (ELISA), sodium dodecyl sulfate-polyacrylamide gel electrophoresis and densitometry (SDS-PAGE/densitometry), and the Bradford assay for total protein. The results were compared to those obtained by immunoblot analysis and …

Predation Of The Chinch Bug, Blissus Occiduus Barber (Hemiptera: Blissidae) By Geocoris Uliginosus (Say) (Hemiptera: Lygaeidae), J. D. Carstens, Frederick P. Baxendale, Tiffany Heng-Moss, Robert J. Wright

Department of Entomology: Faculty Publications

Big-eyed bugs have been well documented as predators on a diverse group of arthropod prey in turfgrasses; however, little is known about the big-eyed bug species associated with buffalograss, or their feeding habits relative to the western chinch bug, Blissus occiduus Barber. This research documented that Geocoris uliginosus (Say) was the predominant big-eyed bug species associated with buffalograss, obtained information on its feeding behavior, and characterized predation rates. Laboratory studies documented G. uliginosus, as a predator of B. occiduus. While all life stages of B. occiduus were attacked by G. uliginosus, predation was greater on 1st through …

A Synopsis Of Nebraska Grasshopper Distributions, Mathew L. Brust, William Wyatt Hoback, Robert J. Wright

Department of Entomology: Faculty Publications

Between 2005 and 2007, 656 new county records and eight new state records were obtained for Nebraska’s short-horned grasshoppers (Acrididae and Romaleidae). This represents an increase of 30% in the known grasshopper county records in Nebraska. Based on the large number of new distribution records, it appears that the previous distributions of Nebraska’s grasshoppers greatly underestimated the actual distributions. The new grasshopper state records were: Boopedon gracile, Chloealtis abdominalis, Encoptolophus subgracilis, Melanoplus bispinosus, Melanoplus borealis, Melanoplus punctulatus, Paratylotropidia brunneri, and Psinidia fenestralis were obtained. These new records will aid in both grasshopper monitoring for pest outbreaks and in conservation of …

Influence Of Temperature On Rate Of Uptake And Subsequent Horizontal Transfer Of [14C]Fipronil By Eastern Subterranean Termites (Isoptera: Rhinotermitidae), Neil A. Spomer, Shripat T. Kamble, Richard A. Warriner, Robert W. Davis

Department of Entomology: Faculty Publications

The effect of temperature on [¹⁴C]fipronil uptake and transfer from donor (D) to recipient (R) Reticulitermes flavipes (Kollar) (Isoptera: Rhinotermitidae) workers was evaluated. Test chambers used in the fipronil uptake study were constructed from petri dishes containing autoclaved soil treated with 1 ppm [¹⁴C]fipronil (1.14 μCi of total radioactivity per petri dish), distilled water, and R. flavipes workers. Test chambers were held in environmental growth chambers preset at 12, 17, 22, 27, and 32°C. For the fipronil transfer study, donor termites stained with Nile blue-A were exposed to soil treated with 1 ppm [¹⁴C]fipronil …

Life In A Temperate Polar Sea: A Unique Taphonomic Window On The Structure Of A Late Cretaceous Arctic Marine Ecosystem, Karen Chin, John Bloch, Arthur Sweet, Justin Tweet, Jaelyn Eberle, Stephen Cumbaa, Jakub Witowski, David M. Harwood

Department of Earth and Atmospheric Sciences: Faculty Publications

As the earth faces a warming climate, the rock record reminds us that comparable climatic scenarios have occurred before. In the Late Cretaceous, Arctic marine organisms were not subject to frigid temperatures but still contended with seasonal extremes in photoperiod. Here, we describe an unusual fossil assemblage from Devon Island, Arctic Canada, that offers a snapshot of a ca 75 _MYR ago marine paleoecosystem adapted to such conditions. Thick siliceous biogenic sediments and glaucony sands reveal remarkably persistent high primary productivity along a high-latitude Late Cretaceous coastline. Abundant fossil feces demonstrate that this planktonic bounty supported benthic invertebrates and large, …

Elevated Primary Productivity Of Calcareous Nannoplankton Associated With Ocean Anxoic Event 1b During The Aptian/Albian Transition (Early Cretaceous), Emily L. Browning, David K. Watkins

Department of Earth and Atmospheric Sciences: Faculty Publications

Previous isotopic investigations of Aptian/Albian oceanic anoxic event (OAE) 1b from the western North Atlantic (Blake Nose) posited that increased sea surface temperatures and decreased salinity led to stratification of the upper water column, resulting in lowered dissolved oxygen and enhanced organic matter preservation. We examined calcareous nannofossils from the same site in the western North Atlantic (Blake Nose) to evaluate changes in surface water conditions prior to, during, and after the Aptian/Albian OAE1b. The results of our analysis conflict somewhat with the previous interpretation that OAE1b at Blake Nose was linked to water column stratification. The signal from calcareous …

Gene Expression Analysis Of Embryonic Stem Cells Expressing Ve-Cadherin (Cd144) During Endothelial Differentiation, Vesna Nikolova-Krstevski, Manoj Bhasin, Hasan H. Otu, Towia A. Libermann, Peter Oettgen

Department of Electrical and Computer Engineering: Faculty Publications

Background: Endothelial differentiation occurs during normal vascular development in the developing embryo. This process is recapitulated in the adult when endothelial progenitor cells are generated in the bone marrow and can contribute to vascular repair or angiogenesis at sites of vascular injury or ischemia. The molecular mechanisms of endothelial differentiation remain incompletely understood. Novel approaches are needed to identify the factors that regulate endothelial differentiation.

Methods: Mouse embryonic stem (ES) cells were used to further define the molecular mechanisms of endothelial differentiation. By flow cytometry a population of VEGF-R2 positive cells was identified as early as 2.5 days after differentiation …

A 20-Year Plan For Hunter/Angler Recruitment, Development And Retention In Nebraska, Mark Pinkerton, Ron Stave, Mick Jensen, James Ziebarth, Jerrod Burke, Lynn Berggren, George Hall, Kent Forney

Department of Electrical and Computer Engineering: Faculty Publications (to 2015)

Hunting and fishing are important parts of Nebraska’s rich heritage. These time-honored outdoor pursuits are important to overall lifestyle, well-being and natural resources of the citizens of Nebraska. The North American Model for Conservation was based primarily on the activism from our nation’s hunters and anglers. Their role helped develop and instill the seven basic premises of the model: Wildlife is to be held in public trust, there should be an elimination of market hunting, wildlife should be allocated by law, there should be hunting/angling opportunities for all citizens, wildlife should only be killed for legitimate reasons, wildlife should be …

Short-Term Memory, Working Memory, And Executive Functioning In Preschoolers: Longitudinal Predictors Of Mathematical Achievement At Age 7 Years, Rebecca Bull, Kimberly Andrews Espy, Sandra A. Wiebe

Department of Educational Psychology: Faculty Publications

This study examined whether measures of short-term memory, working memory, and executive functioning in preschool children predict later proficiency in academic achievement at 7 years of age (third year of primary school). Children were tested in preschool (M age = 4 years, 6 months) on a battery of cognitive measures, and mathematics and reading outcomes (from standardized, norm-referenced school-based assessments) were taken on entry to primary school, and at the end of the first and third year of primary school. Growth curve analyses examined predictors of math and reading achievement across the duration of the study and revealed that …

Contributions Of Children’S Temperament To Teachers’ Judgments Of Social Competence From Kindergarten Through Second Grade, Kathleen Moritz Rudasill, Timothy R. Konold

Department of Educational Psychology: Faculty Publications

Research Findings: Children’s social competence has been linked to successful transition to formal school. The purpose of this study was to examine the contributions of children’s temperament to teachers’ ratings of their social competence from kindergarten through 2nd grade. Children (N = 1,364) from the National Institute of Child Health and Human Development Early Child Care Research Network participated in this study. Mothers rated children’s shyness, attentional focusing, and inhibitory control with the Children’s Behavior Questionnaire at 4½ years, and teachers rated children’s social competence with three subscales (cooperation, assertion, and self-control) of the Social Skills Rating System at …

Cell Autonomous Expression Of Inflammatory Genes In Biologically Aged Fibroblasts Associated With Elevated Nf-Kappab Activity., Andres Kriete, Kelli L Mayo, Nirupama Yalamanchili, William Beggs, Patrick Bender, Csaba Kari, Ulrich Rodeck

Department of Dermatology and Cutaneous Biology Faculty Papers

BACKGROUND: Chronic inflammation is a well-known corollary of the aging process and is believed to significantly contribute to morbidity and mortality of many age-associated chronic diseases. However, the mechanisms that cause age-associated inflammatory changes are not well understood. Particularly, the contribution of cell stress responses to age-associated inflammation in 'non-inflammatory' cells remains poorly defined. The present cross-sectional study focused on differences in molecular signatures indicative of inflammatory states associated with biological aging of human fibroblasts from donors aged 22 to 92 years. RESULTS: Gene expression profiling revealed elevated steady-state transcript levels consistent with a chronic inflammatory state in fibroblast cell-strains …

Demographic Effects Of Canine Parvovirus On A Free-Ranging Wolf Population Over 30 Years, L. David Mech, Sagar M. Goyal, William J. Paul, Wesley E. Newton

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

We followed the course of canine parvovirus (CPV) antibody prevalence in a subpopulation of wolves (Canis lupus) in northeastern Minnesota from 1973, when antibodies were first detected, through 2004. Annual early pup survival was reduced by 70%, and wolf population change was related to CPV antibody prevalence. In the greater Minnesota population of 3,000 wolves, pup survival was reduced by 40–60%. This reduction limited the Minnesota wolf population rate of increase to about 4% per year compared with increases of 16–58% in other populations. Because it is young wolves that disperse, reduced pup survival may have caused reduced …

Weight Changes In Wild Wolves, Canis Lupus, From Ages 2 To 24 Months, L. David Mech

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Weights of 118 female and 141 male Minnesota Wolves (Canis lupus) aged 2-24 months increased almost linearly from about 8 kg for females and 10 kg for males at 3 months to 30 kg for females and 32 kg for males at 10-12 months and then tended to increase much more slowly in an overall curvilinear trend. Considerable variation was apparent for both sexes during their first year.

Spatial And Temporal Differences In Giant Kidney Worm, Dictophyma Renale, Prevalence In Minnesota Mink, Mustela Vison, L. David Mech

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Examination of 110 Mink (Mustela vison) carcasses from 1998 through 2007 indicated that the giant kidney worm, Dioctophyma renale, occurred in Pine and Kanabec Counties of eastern Minnesota with annual prevalences of 0-92%. Worm prevalence increased from 20% in 1999 to 92% in 2001 and decreased to 6% in 2005. During 2000 to 2007, no worms were found in Mink from Anoka and Chisago Counties (n = 54), and in 2000, none in 107 Mink from LeSeur, Freeborn, Redwood, Brown and Watonwan Counties. Changes in kidney worm prevalence were positively related to trapping success, considered an index …

Wolf Body Mass Cline Across Minnesota Related To Taxonomy?, L. David Mech, William J. Paul

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Recent genetic studies suggest that in northern Minnesota two species of wolves (Canis lupus L., 1758 or western wolf and Canis lycaon Schreber, 1775 (= Canis rufus Audubon and Bachman, 1851) or eastern wolf) meet and hybridize. However, little morphological information is available about these two types of wolves in Minnesota. We analyzed the mass of 950 female wolves and 1006 males older than 1 year from across northern Minnesota and found that it increased from 26.30 ± 0.56 kg (mean ± SE) for females and 30.60 ± 0.72 kg for males in northeastern Minnesota to 30.01 ± 0.43 …

Biology And Biological Control Of Exotic True Thistles, Rachel Winston, Rich Hansen, Mark Schwarzlander, Eric Coombs, Carol Bell Randall, Rodney Lym

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

“Thistle” is an old English name for a large variety of weedy, prickly plants that grow throughout the world. The most notable characteristics of thistles are the prickly stems and leaves and the bracts around the flower head. While many different plants have “thistle” in their common name, only certain plant species fit the taxonomic requirements of being considered “true thistles.” True thistle species fall within the family Asteraceae, the tribe Cardueae, and the subtribe Carduinae. Examples of plants that are not true thistles include yellow starthistle (subtribe Centaureinae), sow thistle (subtribe Sonchinae), and Russian thistle (family Chenopodiaceae). Only true …

Evolutionary And Structural Analyses Of Alpha-Papillomavirus Capsid Proteins Yields Novel Insights Into L2 Structure And Interaction With L1, John Lowe, Debasis Panda, Suzanne Rose, Ty Jensen, Willie Hughes, For Yue Tso, Peter C. Angeletti

Nebraska Center for Virology: Faculty Publications

Background: PVs (PV) are small, non-enveloped, double-stranded DNA viruses that have been identified as the primary etiological agent for cervical cancer and their potential for malignant transformation in mucosal tissue has a large impact on public health. The PV family Papillomaviridae is organized into multiple genus based on sequential parsimony, host range, tissue tropism, and histology. We focused this analysis on the late gene products, major (L1) and minor (L2) capsid proteins from the family Papillomaviridae genus Alpha-papillomavirus. Alpha-PVs preferentially infect oral and anogenital mucosa of humans and primates with varied risk of oncogenic transformation. Development of evolutionary associations between …

Early Childhood Infection By Human Herpesvirus 8 In Zambia And The Role Of Human Immunodeficiency Virus Type 1 Coinfection In A Highly Endemic Area, Veenu Minhas, Kay L. Crabtree, Ann Chao, Tendai J. M'Sola, Chipepo Kankasa, Marc Bulterys, Charles D. Mitchell, Charles Wood

Nebraska Center for Virology: Faculty Publications

Kaposi’s sarcoma occurs at high incidence among Zambian adults and children, but there is a paucity of data on human herpesvirus 8 (HHV-8) incidence and routes of infection, especially in children. Between 1998 and 2004, the authors conducted a prospective study of viral transmission in a cohort of 684 children in Lusaka, Zambia, to estimate the annual incidence of HHV-8 from birth through 48 months of age. Maternal and pediatric human immunodeficiency virus type 1 (HIV-1) infection status was also determined. The results, based on 1,532 child-years of follow-up, showed that HHV-8 seroconversion occurs early in life. The incidence rate …

Modified Kigali Combined Staging Predicts Risk Of Mortality In Hiv-Infected Adults In Lusaka, Zambia, Philip J. Peters, Isaac Zulu, Nzali G. Kancheya, Shabir Lakhi, Elwyn Chomba, Cheswa Vwalika, Dhong-Jin Kim, Ilene Brill, Jareen Meinzen-Derr, Amanda Tichacek, Susan A. Allen

Nebraska Center for Virology: Faculty Publications

We assessed the utility of the modified Kigali combined (MKC) staging system for predicting survival in HIV-infected Zambian adults in a prospective, longitudinal, open cohort. From 1995 to 2004, HIV-discordant couples (one HIV-infected partner and one HIV-negative partner) were recruited from couples’ voluntary counseling and testing centers in Lusaka, Zambia and followed at 3-month intervals. MKC stage, which incorporates clinical stage with erythrocyte sedimentation rate (ESR), hematocrit, and body mass index (BMI), was determined at enrollment. Kaplan–Meier survival and Cox proportional hazard methods were used to calculate median survival and relative hazards. We enrolled 1479 HIV-discordant couples with a combined …

Development Of An Immunofluorescence Assay Using Recombinant Proteins Expressed In Insect Cells To Screen And Confirm Presence Of Human Herpesvirus 8-Specific Antibodies, Veenu Minhas, Lynsey N. Crosby, Kay L. Crabtree, Saul Phiri, Tendai J. M'Soka, Chipepo Kankasa, William J. Harrington, Charles D. Mitchell, Charles Wood

Nebraska Center for Virology: Faculty Publications

Human herpesvirus 8 (HHV-8), or Kaposi’s sarcoma (KS)-associated herpesvirus, has been linked to all forms of KS. The results of most current serological assays for the detection of HHV-8-specific antibodies have low levels of concordance among themselves. To establish a sensitive and specific testing strategy that can be used to screen for HHV-8-specific antibodies, three HHV-8 proteins, ORF65, ORF73, and K8.1A, were expressed by using baculoviral vectors in insect cells and incorporated into a monoclonal antibodyenhanced immunofluorescence assay (mIFA) termed the Sf9 three-antigen mIFA. The results obtained by this mIFA were compared to those obtained by a standard mIFA with …

Small-Molecule Cd4 Mimics Interact With A Highly Conserved Pocket On Hiv-1 Gp120, Navid Madani, Arne Schön, Amy M. Princiotto, Judith M. Lalonde, Joel R. Cpurter, Takahiro Soeta, Danny Ng, Liping Wang, Evan T. Brower, Shi-Hua Xiang, Young Do Kwon, Chih-Chin Huang, Richard Wyatt, Peter D. Kwong, Ernesto Freire, Amos B. Smith Iii, Joseph Sodroski

Nebraska Center for Virology: Faculty Publications

Human immunodeficiency virus (HIV-1) interaction with the primary receptor, CD4, induces conformational changes in the viral envelope glycoproteins that allow binding to the CCR5 second receptor and virus entry into the host cell. The small molecule NBD-556 mimics CD4 by binding the gp120 exterior envelope glycoprotein, moderately inhibiting virus entry into CD4-expressing target cells, and enhancing CCR5 binding and virus entry into CCR5-expressing cells lacking CD4. Studies of NBD-556 analogues and gp120 mutants suggest that: 1) NBD-556 binds within the Phe 43 cavity, a highly conserved, functionally important pocket formed as gp120 assumes the CD4- bound conformation; 2) the NBD-556 …

The Human Immunodeficiency Virus Type 1 Envelope Confers Higher Rates Of Replicative Fitness To Perinatally Transmitted Viruses Than To Nontransmitted Viruses, Xiaohong Kong, John T. West, Hong Zhang, Danielle M. Shea, Tendai J. M’Soka, Charles Wood

Nebraska Center for Virology: Faculty Publications

Selection of a minor viral genotype during perinatal transmission of human Immunodeficiency virus type 1

(HIV-1) has been observed, but there is a lack of information on the correlation of the restrictive transmission

with biological properties of the virus, such as replicative fitness. Recombinant viruses expressing the enhanced

green fluorescent protein or the Discosoma sp. red fluorescent (DsRed2) protein carrying the V1 to V5

regions of env from seven mother-infant pairs (MIPs) infected by subtype C HIV-1 were constructed, and

competition assays were carried out to compare the fitness between the transmitted and nontransmitted

viruses. Flow cytometry was used to …

Varying Efficiency Of Long-Term Replication Of Papillomaviruses In Saccharomyces Cerevisiae, Adam J. Rogers, Malte Loggen, Karen Lee, Peter C. Angeletti

Nebraska Center for Virology: Faculty Publications

Human papillomaviruses (HPVs) replicate in mitotically active basal keratinocytes. Two virally encoded proteins, E1, a helicase, and E2, a transcription factor, are important players in replication and maintenance of HPV episomes. We previously showed that HPV16 could replicate stably in Saccharomyces cerevisiae [Angeletti, P.C., Kim, K., Fernandes, F.J., and Lambert, P.F. (2002)] and we identified cis-elements that mediate replication and maintenance [J. Virol. 76(7), 3350-3358.; Kim, K., Angeletti, P.C., Hassebroek, E.C., and Lambert, P.F. (2005)]. Here, we demonstrate that although multiple HPV genomes replicate stably in yeast, they do so with differing long-term efficiency; HPV6-Ura3 is replicated at the …