Digital Commons Network^™

Novel Redox And Dna-Dependent Conformational Changes In Human Ku, A Dna-Double Strand Break Repair Protein, Jason Alexander Lehman

Browse all Theses and Dissertations

Ionizing radiation (IR) and radiomimetic drugs used in cancer chemotherapy cause DNA double-strand breaks which are repaired by the nonhomologous end joining (NHEJ) pathway. Ku is a heterodimeric protein comprised of 70 and 80 kDa subunits and recognizes free DNA ends. Once Ku is bound to DNA, it binds to the DNA-dependent protein kinase catalytic subunit (DNA-PKcs), and forms a heterotrimeric DNA-PK complex for repair and damage signaling.

We have analyzed the Ku protein using multiple biochemical techniques and uncovered a novel reversible redox change (Andrews, Lehman and Turchi, (2006) JBC 281(19):13596-603). From this data, we hypothesized that a redox-dependent …

Estimation Of Evapotranspiration Of Cottonwood Trees In The Cibola National Wildlife Refuge, Cibola, Arizona, Amity J. Jetton

Browse all Theses and Dissertations

This study used sap flow measurements and satellite imagery to estimatewater use by cottonwood (Populus fremontii S. Wats. ssp) trees in an irrigated restoration plot at Cibola National Wildlife Refuge on the Lower Colorado River. Several thousand hectares of irrigated plots of this type are planned to improve riparian habitat on the river, hence it is important to know how much water the trees require. In this study, the ET rates for 20 Freemont cottonwood trees, from an 8 ha plot, were monitored over a 30-day period. ET rates were estimated by measuring sap flow through branches of the trees. …

Validation Of A Custom-Made Microarray To Study Human Intestinal Microflora, Harshavardhan Kenche

Browse all Theses and Dissertations

Intestinal microflora refers to all the different species of bacteria that reside in the human gut and is an important organ of the human body because almost all the digestive reactions of the host occur in the intestine. The bacteria of the intestine play a key role in this process by supplementing the intestine with various enzymes and proteins that are required for the digestive process. At the same time, these bacteria were shown to be implicated in a variety of gastrointestinal disorders like Irritable Bowel Syndrome, Inflammatory Bowel Disorder and Gastrointestinal Cancer, but with the current knowledge about the …

Different Histories But Similar Genetic Diversity And Structure For Black Walnut In Indiana And Missouri, Erin R. Victory, Jeffrey C. Glaubitz, Jennifer A. Fike, Olin E. Rhodes Jr., Keith E. Woeste

Department of Forestry & Natural Resources Faculty Publications

—Missouri and Indiana have markedly different histories of glaciation and recolonization by forest trees. These states also differ in land use patterns and degree of anthropogenic landscape change such as forest fragmentation. To determine the overall effects of these and other demographic differences on the levels of genetic diversity and structure in black walnut (Juglans nigra L.) more than 550 total black walnut trees from nine populations in Indiana and 10 in Missouri were sampled and analyzed using 12 nuclear microsatellite loci. Although genetic diversity parameters such as allelic richness and expected heterozygosity were high overall, they varied little among …

The Jew’S Text: “Shem The Penman” And “Shaun The Post”, Maren Linett

Department of English Faculty Publications

Wyndham Lewis was one of the few early critics of Ulysses to see that, like Stephen Dedalus, Leopold Bloom is a Joycean self-portrait.1 Driven by his anti-Semitism, Lewis was irritated by the sympathy Joyce garnered by portraying himself as a Jew. In Time and Western Man, he complains that Bloom has too much dramatic power: As it is, of course, the author, thinly disguised as a middle-aged Jew tout (Mr. Leopold Bloom), wins the reader’s sympathy every time he appears; and he never is confronted with the less and less satisfactory Dedalus (in the beau role) without the latter losing …

A Bioarchaeological Perspective On Egyptian Colonialism In Nubia During The New Kingdom, Michele Buzon

Department of Anthropology Faculty Publications

During the New Kingdom, Egypt began a military campaign to regain command over Nubia. Extremely successful, their control extended to the fourth cataract by the time of Thutmose III. The circumstances of this power, however, are not well known. It is unclear whether Egyptian colonists or locals administered Nubia during this time. Using a bioarchaeological approach via the study of human skeletal remains and archaeological data from the New Kingdom site of Tombos in Nubia and comparative Egyptian and Nubian skeletal samples, this paper addresses these long-standing questions. Analysesuggest that the Tombos individuals were an ethnically and biologically mixed group. …

The Effects Of Cold Temperature On Gait Initiation And Dynamic Balance In Young And Older Females, Christi Brewer

Electronic Theses and Dissertations

No abstract provided.

Gas-Phonon Interaction Model For Subcontinuum Thermal Transport Simulations, Xiaohui Guo, Dhruv Singh, Jayathi Murthy, Alina A. Alexeenko

School of Aeronautics and Astronautics Faculty Publications

Subcontinuum thermal transport problems such as the contact thermal resistance of semiconductor-gas interactions may play an important role in micro/nano-scale devices. In present work, a gas-phonon interaction modeling approach is suggested based on Boltzmann transport equations. Verification has been conducted by comparisons with asymptotic analytical solutions as well as previously reported numerical results and experimental data. Thermal transpiration in nano-sized channels and thermal resistance of nano-sized constrictions has been studied using the current model Thermal transpiration is the main mechanism for temperature driven pumps in nano/micro-devices. In order to maintain higher temperature ratio between the two ends of the gas …

Microfluidic Delivery Of Small Molecules Into Mammalian Cells Based On Hydrodynamic Focusing, F Wang, H Wang, J Wang, H.-Y. Wang, P L. Rummel, S V. Garimella, C Lu

CTRC Research Publications

Microfluidics-based cell assays offer high levels of automation and integration, and allow multiple assays to be run in parallel, based on reduced sample volumes. These characteristics make them attractive for studies associated with drug discovery. Controlled delivery of drug molecules or other exogenous materials into cells is a critical issue that needs to be addressed before microfluidics can serve as a viable platform for drug screening and studies. In this study, we report the application of hydrodynamic focusing for controlled delivery of small molecules into cells immobilized on the substrate of a microfluidic device. We delivered calcein AM which was …

A New Paradigm For Human Resuscitation Research Using Intelligent Devices, Charles F. Babbs, Andre E. Kemeny, Weilun Quan, Gary Freeman

Weldon School of Biomedical Engineering Faculty Publications

Objectives: To develop new methods for studying correlations between the performance and outcome of resuscitation efforts in real-world clinical settings using data recorded by automatic devices such as automatic external defibrillators (AEDs), and to explore effects of shock timing and chest compression depth in the field. Methods: In 695 records of AED use in the pre-hospital setting, continuous compression data were recorded using AEDs capable of measuring sternal motion during compressions, together with timing of delivered shocks and the electrocardiogram. In patients who received at least one shock, putative return of spontaneous circulation (P-ROSC) was defined as a regular, narrow …

Prediction Of Cooling Heat Transfer Coefficient Of Supercritical Co2 With Small Amount Of Entraned Lubricating Oil Entrained By Neural Network Method, Chaobin Dang, Eiji Hihara

International Refrigeration and Air Conditioning Conference

No abstract provided.

Analyses Of Expressed Sequence Tags From The Maize Foliar Pathogen Cercospora Zeae-Maydis Identify Novel Genes Expressed During Vegetative, Infectious, And Reproductive Growth, Burton H. Bluhm, Braham Dhillon, Erika A. Lindquist, Gert Hj Kema, Stephen B. Goodwin, Larry D. Dunkle

Department of Botany and Plant Pathology Faculty Publications

Background: The ascomycete fungus Cercospora zeae-maydis is an aggressive foliar pathogen of maize that causes substantial losses annually throughout the Western Hemisphere. Despite its impact on maize production, little is known about the regulation of pathogenesis in C. zeae-maydis at the molecular level. The objectives of this study were to generate a collection of expressed sequence tags (ESTs) from C. zeae-maydis and evaluate their expression during vegetative, infectious, and reproductive growth. Results: A total of 27,551 ESTs was obtained from five cDNA libraries constructed from vegetative and sporulating cultures of C. zeae-maydis. The ESTs, grouped into 4088 clusters and 531 …

Teaching Students To Use Company And Industry Information During Their Job Search, Jeremy R. Garritano

Libraries Faculty and Staff Presentations

Libraries and educational institutions provide access to a wide variety of resources that are useful in investigating potential employers, preparing for interviews, and predicting trends in industry. However, often this information is not found in traditional scientific and tech. resources that many chem. and chem. engineering students are familiar with using. This paper will present a no. of strategies for using company and industry resources to look at financial information, industry developments, and opinions of current employees, among other attributes, to better help student prep. themselves for the job market. [ACS Abstract]

Calculating The Volume Of Water Droplets On Topographically-Modified, Micro-Grooved Aluminum Surfaces, Andrew Sommers, Anthony M. Jacobi

International Refrigeration and Air Conditioning Conference

No abstract provided.

Influence Of Pocket Gopher Mounds On Nonnative Plant Establishment In A Shrubsteppe Ecosystem, G. P. Kyle, Andrew Kulmatiski, Karen H. Beard

Wildland Resources Faculty Publications

Soil disturbances across a wide range of spatial scales have been found to promote the establishment of invasive plant species. This study addresses whether mounds built by northern pocket gophers (Thomomys talpoides) in the shrubsteppe environment of north central Washington are facilitating plant invasions into native-dominated fields. Research was conducted in native-dominated plant communities adjacent to ex-arable, nonnative-dominated fields. To determine the effect of mounds on plant growth, we recorded new establishment and persistence of all plant species over 2 growing seasons on 10–19 mound and intermound areas in 10 fields. Nonnative plant establishment was not affected by mounds, but …

Aging Predisposes Oocytes To Meiotic Nondisjunction When The Cohesin Subunit Smc1 Is Reduced, Vijayalakshmi V. Subramanian, Sharon E. Bickel

Dartmouth Scholarship

In humans, meiotic chromosome segregation errors increase dramatically as women age, but the molecular defects responsible are largely unknown. Cohesion along the arms of meiotic sister chromatids provides an evolutionarily conserved mechanism to keep recombinant chromosomes associated until anaphase I. One attractive hypothesis to explain age- dependent nondisjunction (NDJ) is that loss of cohesion over time causes recombinant homologues to dissociate prematurely and segregate randomly during the first meiotic division. Using Drosophila as a model system, we have tested this hypothesis and observe a significant increase in meiosis I NDJ in experimentally aged Drosophila oocytes when the cohesin protein SMC1 …

Reducing Estate And Trust Litigation Through Disclosure, In Terrorem Clauses, Mediation And Arbitration, Jonathan G. Blattmachr

Cardozo Journal of Conflict Resolution

This Article discusses how litigation relating to the administration of estates and trusts might be reduced through by careful planning prior to the property owner's transfer of wealth during lifetime or at death. As the article details, litigation involving trust and estate matters often involves an emotional element not present in most other legally disputed matters. That also suggests that methods to reduce the risk of such litigation occurring may be unique to such matters. In Part 1, certain background matters relating to the climate for such litigation are presented. In Part 2, the historic "tool" of a disinheritance clause …

Adr And Collective Trauma: Constructing The Forum For The Traumatic Fuss, Michal Alberstein

Cardozo Journal of Conflict Resolution

This paper explores the notion of collective trauma and discusses the implications of its theoretical underpinnings in two Alternative Dispute Resolution (ADR) practices. First, it offers the use of the Narrative Mediation model to construct a method to deal with collective trauma, as it affects individuals. Narrative Mediation is presented as the most comprehensive and transformative tool to address collective trauma. Second, an ADR approach to collective trauma is offered in cases where no mediation is possible. The concept of "fitting the forum to the fuss," which is a fundamental organizing mechanism in the ADR field, is applied in this …

Understanding Indigenous Dispute Resolution Processes And Western Alternative Dispute Resolution: Cultivating Culturally Appropriate Methods In Lieu Of Litigation, Carlo Osi

Cardozo Journal of Conflict Resolution

This article specifically focuses on Indigenous Dispute Resolution, Western-inspired ADR mechanisms, and Indigenized Western ADR as culturally appropriate methods to resolve disputes in lieu of litigation. This article will also focus on how these processes are able to help solve intellectual property rights disputes involving Indigenous communities. Generally, this article will describe how successful all three modes of dispute resolution have been in Indigenous conflict resolution, in the quest for benefit-sharing agreements, and the potential for their use in other controversies. This article proposes the adoption of an Indigenized Western ADR approach on issues surrounding intellectual property rights regimes.

Use Of Palm Trees As A Sleeping Site By Hamadryas Baboons In Ethiopia, Amy Schreier, Larissa Swedell

Publications and Research

Hamadryas baboons sleep on cliffs throughout their range, and this can be attributed to the safety cliffs provide against predators in the absence of tall trees. In this paper, we report the first documented occurrence of hamadryas baboons sleeping in doum palm trees rather than on cliffs. Data derive from a study of hamadryas baboons at the Filoha site in lowland Ethiopia. During all-day follows, data were collected on travel patterns, band activity, and location. Variation in the baboons’ home range was characterized using vegetation transects. We discovered that one band in this population, Band 3, occasionally slept in doum …

Composition And Seasonality Of Diet In Wild Hamadryas Baboons: Preliminary Findings From Filoha, Larissa Swedell, Getenet Hailemeskel, Amy Schreier

Publications and Research

Here we report the first year-round quantitative data on dietary composition and seasonality in wild hamadryas baboons. Study subjects were adult male members of Band 3 at the Filoha field site in central lowland Ethiopia. Data collection consisted of 10-minute focal samples during all-day follows 4-6 days per month over the course of one year. The two largest contributors to the diet were Hyphaene thebaica and Acacia senegal, and these were the only plant species found in the diet during every month of the year. Other relatively major contributors to the diet, such as Cyperus grandibulbosus, Seddera bagshawei, …

Negative Dimensions Of Identity: A Research Agenda For Law And Public Policy, Avner Ben-Ner, Claire A. Hil

Minnesota Journal of Law, Science & Technology

No abstract provided.

Deer Impact On Vegetation In Natural Areas In Southeastern Nebraska, Joseph Gubanyi, Julie A. Savidge, Scott E. Hygnstrom, Kurt C. Vercauteren, Gary W. Garabrandt, Seth Korte

United States Department of Agriculture Wildlife Services: Staff Publications

We studied the effect of browsing by white-tailed deer (Odocoileus virginianus Zimmerman) on vegetation at three sites with high densities of deer and six sites with low densities of deer along the Missouri and Platte Rivers in southeastern Nebraska in 1995-1996. Vegetation volume from 0-1 m high was similar between sites with high and low deer densities (P > 0.05). From 1-2 m high, vegetation volume was less at sites with more deer in both years (P < 0.05). Densities for eight of 11 small woody plant categories (< 3 cm dbh) were less common at sites with high densities of deer (P < 0.05). Overall densities of small trees (3-15 cm dbh) were similar between sites with high and low densities of deer. However, small hackberry trees (Celtis occidentalis L.) were more common (P = 0.03), while all other small trees were less common (P = 0.038), at sites with …

Passive Immunity To West Nile Virus Provides Limited Protection In A Common Passerine Species, Nicole M. Nemeth, Paul T. Oesterle, Richard A. Bowen

United States Department of Agriculture Wildlife Services: Staff Publications

Passerine birds have played an important role in the establishment, maintenance, and spread of West Nile virus (WNV) in North America, and some are susceptible to WNV-associated mortality. Characterization of passive transfer of anti-WNV antibodies in passerines is important to understanding transmission and demographic effects of WNV on wild birds. We showed passively acquired maternal antibodies to WNV in the house sparrow (Passer domesticus). Although all seropositive females (N = 18) produced antibody-positive egg yolks, only 20% of seropositive mothers (3/15) produced seropositive chicks. The estimated average half-life of maternal antibodies in chick sera was 3 days, …

Skunk Rabies In California (1992–2003)—Implications For Oral Rabies Vaccination, Ray T. Sterner, Ben Sun, Jean B. Bourassa, Robert L. Hale, Stephanie A. Shwiff, Michele T. Jay, Dennis Slate

United States Department of Agriculture Wildlife Services: Staff Publications

Skunk-variant rabies is endemic in California (United States), and the development of oral vaccines and baits to vaccinate skunks is in progress. In 2003, the California Department of Public Health (CDPH) began to quantify the impacts of skunk-variant rabies and to assess the feasibility of using oral rabies vaccination (ORV) as a containment measure. The CDPH rabies case data for skunks were spatially depicted and analyzed using a geographic information system. Statewide, rabid skunks (1992–2003) primarily occurred in seven physiographic regions: Central Coast, North Coast, North Sierra, Sacramento Valley, San Francisco Bay and Delta, San Joaquin Valley, and South Sierra. …

Nuclear Magnetic Resonance Investigation Of Loci Of Solubilization And The Effect Of Ionic Strength Changes, Olumuyiwa Olaotan Olaosun

Electronic Theses and Dissertations

No abstract provided.

Greenhouse Gas Emissions From Two Soils Receiving Nitrogen Fertilizer And Swine Manure Slurry, Marek K. Jarecki, Timothy B. Parkin, Alvarus S. K. Chan, Jerry L. Hatfield, Raymond Jones

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The interactive effects of soil texture and type of N fertility (i.e., manure vs. commercial N fertilizer) on N₂O and CH₄ emissions have not been well established. Th is study was conducted to assess the impact of soil type and N fertility on greenhouse gas fluxes (N₂O, CH₄, and CO₂) from the soil surface. The soils used were a sandy loam (789 g kg⁻¹ sand and 138 g kg⁻¹ clay) and a clay soil (216 g kg⁻¹ sand, and 415 g kg⁻¹ clay). Chamber experiments were conducted …

Insect-Attracting And Antimicrobial Properties Of Antifreeze For Monitoring Insect Pests And Natural Enemies In Stored Corn, Xinzhi Ni, Gunawati Gunawan, Steve L. Brown, Paul E. Sumner, John R. Ruberson, G. David Buntin, C. Corley Holbrook, R. Dewey Lee, Douglas A. Streett, James E. Throne, James F. Campbell

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Insect infestations in stored grain cause extensive damage worldwide. Storage insect pests, including the Indianmeal moth, Plodia interpunctella (Hubner) (Lepidoptera: Pyralidae); Sitophilus spp. (Coleoptera: Curculionidae); and their natural enemies [e.g., Cephalonomia tarsalis (Ashmead) (Hymenoptera: Bethylidae), and Anisopteromalus calandrae (Howard) (Hymenoptera: Pteromalidae)] inhabit a temporary, but stable ecosystem with constant environmental conditions. The objective of the present experiment was to assess the efficacy of using ethylene glycol antifreeze in combination with nutrient solutions to monitor storage insect pest and natural enemy populations in three bins of corn, Zea mays L. The treatments were deionized water, a diluted (1:5 antifreeze:water) antifreeze solution, …

The Kinetics Of Neutralizing Anti-Viral Antibodies And The Production Of A T Cell-Dependent Antibody Response During Vesicular Stomatitis Virus Infection, Emily Claire Pyle

All NMU Master's Theses

No abstract provided.

The Characterization And In Vitro Silencing Of The Oncogene Zbtb7a In Malignant Glioma, Justin Joseph Segula

All NMU Master's Theses

No abstract provided.