Digital Commons Network^™

A Set Of Phosphatase-Inert “Molecular Rulers” To Probe For Bivalent Mannose 6-Phosphate Ligand-Receptor Interactions, Xiang Fei, Christopher M. Connelly, Richard G. Macdonald, David B. Berkowitz

David Berkowitz Publications

A set of bivalent mannose 6-phosphonate “molecular rulers” has been synthesized to examine ligand binding to the M6P/IGF2R. The set is estimated to span a P-P distance range of 16–26 Å (MMFF energy minimization on the hydrated phosphonates). Key synthetic transformations include sugar triflate displacement for phosphonate installation and Grubbs I cross-metathesis to achieve bivalency. Relative binding affinities were tested by radioligand displacement assays versus PMP-BSA (pentamannose phosphate-bovine serum albumin). These compounds exhibit slightly higher binding affinities for the receptor (IC_50’s = 3.7–5 μM) than the parent, monomeric mannose 6-phosphonate ligand and M6P itself (IC₅₀ = 11.5 ± …

Louisiana State University Health Sciences Center - 2008 - 134th Commencement, Office Of The Registrar, Lsu Health Sciences Center-No

Graduation & Commencement Programs

The program for the 134th Commencement of LSU Health Sciences Center - New Orleans held May 15, 2008.

Evidence In The Carotenoid Biosynthetic Pathway For Variation In Evolutionary Rates, Stephanie A. Anderson

Biology Honors Theses

Many cellular reactions occur in linear metabolic pathways where gene products act sequentially to produce needed compounds. The interrelationships between the products of these loci raises the question of whether they evolve in concert or independently. Previous research addressing this question indicated that in the anthocyanin pathway, which produces important secondary metabolites in plants, the genes encoding downstream enzymes show an accelerated rate of evolution when compared to upstream loci. The hypothesized cause of these differences has been attributed to relaxed selective constraint. This pattern and process has not, however, been tested in other systems. The carotenoid biosynthetic pathway, which …

Central Florida Future, Vol. 40 No. 47, May 15, 2008

Central Florida Future

Colbourn burglarized on exam week; Our roots grow deep; Medical school makes history; Looking to wrap UCF up.

388 A Structure Of Cytoplasmic Polyhedrosis Virus By Cryo-Electron Microscopy, Xuekui Yu, Lei Jin, Z Hong Zhou

Faculty, Staff and Student Publications

Cytoplasmic polyhedrosis virus (CPV) is unique within the Reoviridae family in having a turreted single-layer capsid contained within polyhedrin inclusion bodies, yet being fully capable of cell entry and endogenous RNA transcription. Biochemical data have shown that the amino-terminal 79 residues of the CPV turret protein (TP) is sufficient to bring CPV or engineered proteins into the polyhedrin matrix for micro-encapsulation. Here we report the three-dimensional structure of CPV at 3.88 A resolution using single-particle cryo-electron microscopy. Our map clearly shows the turns and deep grooves of alpha-helices, the strand separation in beta-sheets, and densities for loops and many bulky …

Energization-Dependent Endogenous Activation Of Proton Conductance In Skeletal Muscle Mitochondria., Nadeene Parker, Charles Affourtit, Antonio Vidal-Puig, Martin D. Brand

School of Biomedical Sciences

Leak of protons into the mitochondrial matrix during substrate oxidation partially uncouples electron transport from phosphorylation of ADP, but the functions and source of basal and inducible proton leak in vivo remain controversial. In the present study we describe an endogenous activation of proton conductance in mitochondria isolated from rat and mouse skeletal muscle following addition of respiratory substrate. This endogenous activation increased with time, required a high membrane potential and was diminished by high concentrations of serum albumin. Inhibition of this endogenous activation by GDP [classically considered specific for UCPs (uncoupling proteins)], carboxyatractylate and bongkrekate (considered specific for the …

Highlights In Jazz Concert 288- An Evening With Dave Brubeck, Jack Kleinsinger, Danny Gottlieb

Jack Kleinsinger Presents Highlights in Jazz

Jack Kleinsinger presents Highlights in Jazz. The concert was held at The Tribeca Performing Arts Center, Thursday, May 15th, 2008 at 8:00 pm. Jack served as producer and master of ceremonies for the series of concerts. Artists for the concert include Dave Brubeck, Bobby Millitello, Michael Moore, Randy Jones, and surprise guests Hilliard Greene and Freddy Bryant.

The Portland Spectator, May 2008, Portland State University. Student Publications Board

Portland Spectator

Editor: Tessie M. Lopez

Articles in this issue include: Dirty politics: A play-by-play of a Portland Mayoral debate at Portland State University; Environmental arrogance: An exploration into the effects of global environment efforts; Nuclear power: Discussion on the forgotten ‘clean’ power; and Ecological economics: Questions on whether one person can really make a difference on the environment.

Washington University Record, May 15, 2008

Washington University Record

No abstract provided.

Exercise Induced Alterations In Rat Monocyte Number, Morphology, And Function, Marcia Guereschi†, Jonato Prestes‡, Felipe Donatto‡, Rodrigo Dias‡, Anelena Frollini‡, Clílton Ferreira‡, Claudia Cavaglieri‡, Adrianne Palanch‡

International Journal of Exercise Science

The purpose of this study was to verify the histophysiological alterations in monocytes and macrophages induced by short periods of exercise. Male Wistar rats (age = 2 months, body weight = 200g) were divided into seven groups (N=6 each): sedentary control (C), groups exercised (swimming) at low intensity for 5 (5L), 10 (10L), and 15 minutes (15L), and groups exercised at moderate intensity for 5 (5M), 10 (10M) or 15 minutes (15M). At moderate intensity the animals carried a load of 5% of body weight on their backs. Blood monocytes were evaluated for quantity and morphology, and peritoneal macrophages were …

Observer, Central Washington University

Student Newspaper

Student newspaper for Central Washington University for May 14, 2008

Volume 45, Number 38: May 14, 2008, University Of North Dakota

University Letter Archive

No abstract provided.

Mustang Daily, May 14, 2008

Cal Poly Student Newspaper

Student newspaper of California Polytechnic State University, San Luis Obispo, CA.

The Dark Side Of Recycling And Reusing Electronics: Is Washington's E-Cycle Program Adequate?, Nicola J. Templeton

Seattle Journal for Social Justice

No abstract provided.

Sticky Gecko Feet: The Role Of Temperature And Humidity, Peter H. Niewiarowski, Stephanie Lopez, Liehui Ge, Emily Hagan, Ali Dhinojwala

College of Polymer Science and Polymer Engineering

Gecko adhesion is expected to be temperature insensitive over the range of temperatures typically experienced by geckos. Previous work is limited and equivocal on whether this expectation holds. We tested the temperature dependence of adhesion in Tokay and Day geckos and found that clinging ability at 12 degrees C was nearly double the clinging ability at 32 degrees C. However, rather than confirming a simple temperature effect, our data reveal a complex interaction between temperature and humidity that can drive differences in adhesion by as much as two-fold. Our findings have important implications for inferences about the mechanisms underlying the …

A Specific Role For Ca2+-Dependent Adenylyl Cyclases In Recovery From Adaptive Presynaptic Silencing, Krista L. Moulder, Xiaoping Jiang, Chunyun Chang, Amanda A. Taylor, Ann M. Benz, Alana C. Conti, Louis J. Muglia, Steven Mennerick

Neurosurgery Faculty Publications

Glutamate generates fast postsynaptic depolarization throughout the CNS. The positive-feedback nature of glutamate signaling likely necessitates flexible adaptive mechanisms that help prevent runaway excitation. We have previously explored presynaptic adaptive silencing, a form of synaptic plasticity produced by ongoing neuronal activity and by strong depolarization. Unsilencing mechanisms that maintain active synapses and restore normal function after adaptation are also important, but mechanisms underlying such presynaptic reactivation remain unexplored. Here we investigate the involvement of the cAMP pathway in the basal balance between silenced and active synapses, as well as the recovery of baseline function after depolarization-induced presynaptic silencing. Activation of …

Sticky Gecko Feet: The Role Of Temperature And Humidity, Peter H. Niewiarowski, Stephanie Lopez, Liehui Ge, Emily Hagan, Ali Dhinojwala

Biology Faculty Research

Gecko adhesion is expected to be temperature insensitive over the range of temperatures typically experienced by geckos. Previous work is limited and equivocal on whether this expectation holds. We tested the temperature dependence of adhesion in Tokay and Day geckos and found that clinging ability at 12 degrees C was nearly double the clinging ability at 32 degrees C. However, rather than confirming a simple temperature effect, our data reveal a complex interaction between temperature and humidity that can drive differences in adhesion by as much as two-fold. Our findings have important implications for inferences about the mechanisms underlying the …

Natives, Nostalgia, And Nature In Children's Popular Film Narratives, C. Richard King

CLCWeb: Comparative Literature and Culture

In his paper "Natives, Nostalgia, and Nature in Children's Popular Film Narratives," C. Richard King offers a critical reading in an effort to appreciate the entanglements of nature, natives, and nostalgia in children's narratives. In this context, an analysis of Spirit: Stallion of the Cimarron Spirit affords an ideal occasion for such a reading because it centers on the relations between Native Americans, Euroamericans, and the natural world as it tells the story of a wild mustang living in western North America in the late nineteenth century. Indeed, as close examination reveals, the film uses race, gender, civilization, and wildness …

Race And Gender Representations In Advertising In Cable Cartoon Programming, Debra L. Merskin

CLCWeb: Comparative Literature and Culture

In her paper "Race and Gender Representations in Advertising in Cable Cartoon Programming," Debra L. Merskin explores what children see and what they learn about racial, ethnic, and gender identity in television advertisements. Merskin examines race and ethnicity in commercials on cable television, specifically on the Turner Cartoon Network, an environment devoted entirely to cartoons and to children. Her content analysis of 381 advertisements reveals that while White and African American children are overrepresented in relationship to their percentage of the U.S. population, other minority group children are rarely portrayed. In only one instance were children of color shown alone, …

Magassa, Bandiougou And Fofana, Lassana, Bronx African American History Project

Oral Histories

Interviewer: Mark Naison

Interviewees: Lassana Fofana, Bandiogou Magassa

Summarized By: Eddie Mikus

Lassana Fofana and Bandiogou Magassa are two immigrants to the Bronx from the African country of Mal. Their story stands as an example of the challenges that immigrants face when arriving in a new country.

Fofana and Magassa grew up in a rural part of Mali which did not have electricity or television. Fofana attended school in Mali up until the ninth grade while Magassa attended through the 12^th grade (although Magassa enrolled in Martin Luther King High School after arriving in the United States). Magassa cited …

Polly Bemis, Pedagogy, And Multiculturalism In The Classroom, Katy Fry

CLCWeb: Comparative Literature and Culture

As Katy Fry suggests in her paper "Polly Bemis, Pedagogy, and Multiculturalism in the Classroom," the history of the US-American West is too often taught in mythological terms. This is especially true in grade school classrooms, where children are told that the West was settled by courageous men and women who dared to come to and conquer a wild, untamed place. The notion of what it meant to be a pioneer remains simple and uncomplicated. As Fry points out, however, there were pioneers of a different sort, such as the Chinese immigrant Polly Bemis. Fry's article examines the various pedagogic …

Introduction To Racialized Narratives For Children, C. Richard King

CLCWeb: Comparative Literature and Culture

No abstract provided.

Differences In Preferences Towards The Environment: The Impact Of A Gender, Age And Parental Effect, Benno Torgler, María A. García Valiñas, Alison Macintyre

Fondazione Eni Enrico Mattei Working Papers

The paper investigates empirically the differences in preferences towards protection of the environment. Using seven different dependent variables to focus on the impact of age, gender and children we use a large micro data set covering data from 33 Western and Eastern European countries. The results indicate that women have both a stronger preference towards the environment and a stronger willingness to contribute. Moreover, we observe the tendency of a negative correlation between age and environmental preferences. However, a positive effect is visible once we focus on the impact of age on social norms (environmental morale). Finally, we were not …

Preserving Sacred Places: Free Exercise And Historic Preservation In The Context Of Third Church Of Christ, Scientist, Washington, Dc, Bryan Stockton

Georgetown Law Historic Preservation Papers Series

This paper will analyze the tension between the historic preservation of sacred places and the free exercise of religion as seen through the recent controversy surrounding the landmarking of Third Church of Christ, Scientist, in Washington, D.C. Assuming Third Church would bring a free exercise and RLUIPA challenge if the District denied a demolition permit, this paper will examine how such a suit would likely fail.

After describing the factual background, the paper will evaluate questions of standing and ripeness. The mere fact of landmarking does not create a cause of action recognized by District of Columbia courts, so any …

Over My Dead Property! Why The Owner Consent Provisions Of The National Historic Preservation Act Strike The Wrong Balance Between Private Property And Preservation, Jess Theodore

Georgetown Law Historic Preservation Papers Series

It is not unusual for an article about the tension between property rights and historic preservation to begin with a gloomy scenario. One day, you, the private property owner, receive a knock at the door. Upon opening the door, you discover an ominous government official who demands that you immediately stop construction on the addition to your home.

Flabbergasted, you begin to protest, naming the many reasons why the addition must be built. But, the official does not care that the quintuplets have outgrown your present home. Neither is he sympathetic when you tell him that you paid fair market …

Mustang Daily, May 13, 2008

Cal Poly Student Newspaper

Student newspaper of California Polytechnic State University, San Luis Obispo, CA.

Selfishness As Second-Order Altruism, Omar Tonsi Eldakar, David Sloan Wilson

Biology Faculty Articles

Selfishness is seldom considered a group-beneficial strategy. In the typical evolutionary formulation, altruism benefits the group, selfishness undermines altruism, and the purpose of the model is to identify mechanisms, such as kinship or reciprocity, that enable altruism to evolve. Recent models have explored punishment as an important mechanism favoring the evolution of altruism, but punishment can be costly to the punisher, making it a form of second-order altruism. This model identifies a strategy called “selfish punisher” that involves behaving selfishly in first-order interactions and altruistically in second-order interactions by punishing other selfish individuals. Selfish punishers cause selfishness to be a …

Catalytic Wet Oxidation Of Lactose, Joseph Holles

Michigan Tech Patents

A process for converting lactose into carbon dioxide and/or carbon monoxide using catalytic wet oxidation. Oxygen gas and an aqueous solution of lactose are fed to a reactor comprising a Pt/Al203 catalyst, a Mn/Ce catalyst or a Pt/Mn—Ce catalyst, and the lactose is oxidized in the reactor at elevated temperature and pressure to produce at least one of small organic acids, carbon dioxide, carbon monoxide, water and combinations thereof. The small organic acids may be further degraded by feeding the small organic acids and oxygen gas into a reactor containing a Mn/Ce catalyst and oxidizing the small organic acids to …

The Role Of Mitochondria Atp-Sensitive Potassium Channel In Cardioprotection, Ksenia Andrukhiv Everton

Dissertations and Theses

Mitochondrial ATP-sensitive potassium channel (mitoK_ATP) has been suggested to be the mediator of cardiac preconditioning. All of the diverse pharmacological and physiological agents that open mitoK_ATP provide protection against ischemia-reperfusion injury. Some investigators have pointed out that some of these mitoK_ATP channel openers also inhibit succinate dehydrogenase (SDH), complex II of the electron transport chain. Based on this observation they suggest that it is the inhibition of SDH, and not the opening of mitoK_ATP channel, that mediates the observed cardioprotection. In this study, I examined four chemically distinct and unrelated pharmacological agents, diazoxide, 3-Nitropropionic Acid …

Recombinant Mycobacteria Overexpressing D-Alanine Ligase Gene And Uses Therefore: United States Patent No. Us 7,371,571 B2, Raul G. Barletta, Zhengyu Feng

School of Veterinary and Biomedical Sciences: Faculty Publications

Recombinant mycobacterial strains which overproduce essential biosynthetic enzymes of pathogenic mycobateria are provided. These strains overproduce enzymes involved in the synthesis and incorporation of D-alanine into mycobacterial peptidoglycan, the backbone of the mycobacterial cell wall. These overproducing strains may be used as reference strains in in vitro screening methods to identify antimycobacterial agents.