Digital Commons Network^™

The Effects Of Seeding Rate Of Prairie Grasses And Forbs On Native Plant Establishment In A Prairie Reconstruction. Iowa Department Of Transportation Final Report., Dave W, Williams, Daryl D. Smith

Faculty Work

The Iowa DOT is seeking ways to improve practices associated with revegetation projects to increase establishment of native grasses and forbs. Iowa DOT has developed various seed mixes for re-vegetation projects (Iowa DOT 2005). Each seed mix has the potential to result in a different plant community. Results from this research project will provide information on the seeding rates for species needed for optimal establishment of grasses and wildflowers in future Iowa DOT prairie plantings as well as determine if current seeding practices are adequate. This information can be used to revise or develop seeding rate recommendations for revegetation projects.

Fisheries Occasional Publication 40: A Summary Of Data Collected By The Angler’S Daily Log Book And Fishing Tournament Monitoring Programs In 2004-2006, Kimberley A. Smith, M Hammond, Joshua Brown

Fisheries Occasional Publications

Catch information and biological samples collected by recreational anglers in daily log books can provide valuable information about local fish stocks. The numbers and lengths of important recreational target species can be used to monitor their abundance and population structure, which can form the basis of fishery stock assessments. In addition, the total composition of the catch and observations about fish health provide measures of local biodiversity and ecosystem health. The Department of Fisheries (DoF) Recreational Angler’s Daily Log Book Program was launched in March 2004 as part of the DoF Research Angler Program (RAP). The current log book format …

Reading, Writing, And Race: The Constitutionality Of Educational Strategies Designed To Teach Racial Literacy, Michael J. Kaufman

University of Richmond Law Review

No abstract provided.

The Modulation Of Stat5a/Gr Complexes During Fat Cell Differentiation And In Mature Adipocytes, James E. Baugh, Z. Elizabeth Floyd, Jacqueline M. Stephens

Faculty Publications

Objective: Signal transducer and activator of transcription (STAT) 5A has been shown to interact with the glucocorticoid receptor (GR) in adipocytes. The aim of this study was to investigate the subcellular locations and modulation of STAT5A/GR complexes during adipogenesis and in mature adipocytes. Research Methods and Procedures: Both 3T3-L1 and 3T3-F442A cells were studied by performing subcellular fractionations, immunoprecipitation, and Western blotting after various treatments. Results: The formation of nuclear STAT5A/GR complexes was regulated in the cytosol and in the nucleus at distinct times during adipogenesis and in mature adipocytes. STAT5A, but not STAT5B, forms a complex with GR in …

Landscape Context Outweighs Local Habitat Quality In Its Effects On Herbivore Dispersal And Distribution, Kyle J. Haynes, Forrest P. Dillemuth, Bryan J. Anderson, Alyssa S. Hakes, Heather B. Jackson, S. Elizabeth Jackson, James T. Cronin

Faculty Publications

Past studies with spatially structured herbivore populations have emphasized the primacy of intrinsic factors (e.g., patch quality), patch geometry (e.g., patch size and isolation), and more recently landscape context (e.g., matrix composition) in affecting local population abundance and dispersal rate. However, few studies have examined the relative importance of each factor, or how they might interact to affect herbivore abundance or dispersal. Here, we performed a factorial field experiment to examine the independent and interactive effects of patch quality (plant biomass, leaf protein, leaf phenolics) and matrix composition [mudflat or non-host grass (Bromus inermis)] on planthopper (Prokelisia crocea) emigration from …

A Genetic Screen Supports A Broad Role For The Drosophila Insulator Proteins Beaf-32a And Beaf-32b In Maintaining Patterns Of Gene Expression, Swarnava Roy, Yian Yee Tan, Craig M. Hart

Faculty Publications

Chromatin domain insulators are thought to insulate adjacent genes, including their regulatory elements, from each other by organizing chromatin into functionally independent domains. Thus insulators should play a global role in gene regulation by keeping regulatory domains separated. However, this has never been demonstrated. We previously designed and characterized a transgene that is under GAL4 UAS control and encodes a dominant-negative form of the Boundary Element-Associated Factors BEAF-32A and BEAF-32B. The BID transgene encodes the BEAF self-interaction domain but lacks a DNA binding domain. Expression of BID in eye imaginal discs leads to a rough eye phenotype. Here we screen …

Alkalilimnicola Ehrlichii Sp. Nov., A Novel, Arsenite-Oxidizing Haloalkaliphilic Gammaproteobacterium Capable Of Chemoautotrophic Or Heterotrophic Growth With Nitrate Or Oxygen As The Electron Acceptor, Shelley E. Hoeft, Jodi Switzer Blum, John F. Stolz, F. Robert Tabita, Brian Witte, Gary M. King, Joanne M. Santini, Ronald S. Oremland

Faculty Publications

A facultative chemoautotrophic bacterium, strain MLHE-1T, was isolated from Mono Lake, an alkaline hypersaline soda lake in California, USA. Cells of strain MLHE-1T were Gram-negative, short motile rods that grew with inorganic electron donors (arsenite, hydrogen, sulfide or thiosulfate) coupled with the reduction of nitrate to nitrite. No aerobic growth was attained with arsenite or sulfide, but hydrogen sustained both aerobic and anaerobic growth. No growth occurred when nitrite or nitrous oxide was substituted for nitrate. Heterotrophic growth was observed under aerobic and anaerobic (nitrate) conditions. Cells of strain MLHE-1T could oxidize but not grow on CO, while CH4 neither …

Color-Blind Justice: Albion Tourge And The Quest For Racial Equality From The Civil War To Plessy V. Ferguson, Jane Dailey

Civil War Book Review

One Man's Search for Equality in America

A Mobile Element-Based Evolutionary History Of Guenons (Tribe Cercopithecini), Jinchuan Xing, Hui Wang, Yuhua Zhang, David A. Ray, Anthony J. Tosi, Todd R. Disotell, Mark A. Batzer

Faculty Publications

Background: Guenons (tribe Cercopithecini) are a species-rich group of primates that have attracted considerable attention from both primatologists and evolutionary biologists. The complex speciation pattern has made the elucidation of their relationships a challenging task, and many questions remain unanswered. SINEs are a class of non-autonomous mobile elements and are essentially homoplasy-free characters with known ancestral states, making them useful genetic markers for phylogenetic studies. Results: We identified 151 novel Alu insertion loci from 11 species of tribe Cercopithecini, and used these insertions and 17 previously reported loci to infer a phylogenetic tree of the tribe Cercopithecini. Our results robustly …

Human Genomic Dna Quantitation System, H-Quant: Development And Validation For Use In Forensic Casework, Jaiprakash G. Shewale, Elaine Schneida, Jonathan Wilson, Jerilyn A. Walker, Mark A. Batzer, Sudhir K. Sinha

Faculty Publications

The human DNA quantification (H-Quant) system, developed for use in human identification, enables quantitation of human genomic DNA in biological samples. The assay is based on real-time amplification of AluYb8 insertions in hominoid primates. The relatively high copy number of subfamily-specific Alu repeats in the human genome enables quantification of very small amounts of human DNA. The oligonucleotide primers present in H-Quant are specific for human DNA and closely related great apes. During the real-time PCR, the SYBR® Green I dye binds to the DNA that is synthesized by the human-specific AluYb8 oligonucleotide primers. The fluorescence of the bound SYBR® …

Requirement Of Nhp6 Proteins For Transcription Of A Subset Of Trna Genes And Heterochromatin Barrier Function In Saccharomyces Cerevisiae, Priscilla Braglia, Sandra L. Dugas, David Donze, Giorgio Dieci

Faculty Publications

A key event in tRNA gene (tDNA) transcription by RNA polymerase (Pol) III is the TFIIIC-dependent assembly of TFIIIB upstream of the transcription start site. Different tDNA upstream sequences bind TFIIIB with different affinities, thereby modulating tDNA transcription. We found that in the absence of Nhp6 proteins, the influence of the 5′-flanking region on tRNA gene transcription is dramatically enhanced in Saccharomyces cerevisiae. Expression of a tDNA bearing a suboptimal TFIIIB binding site, bat not of a tDNA preceded by a strong TFIIIB binding region, was strongly dependent on Nhpf6 in vivo. Upstream sequence-dependent stimulation of tRNA gene transcription by …

The Pan American (2007-03-01), Claudette Gonzalez

UTPA Archives - The Pan American Newspaper

56th Year, No. 21

Effects Of Ice Melting On Grace Observations Of Ocean Mass Trends, Don P. Chambers, Mark E. Tamisiea, R Steven Nerem, John C. Ries

Marine Science Faculty Publications

The Gravity Recovery and Climate Experiment (GRACE) was designed to measure variations in the Earth's gravity field from space at monthly intervals. Researchers have used these data to measure changes in water mass over various regions, including the global oceans and continental ice sheets covering Greenland and Antarctica. However, GRACE data must be smoothed in these analyses and the effects of geocenter motions are not included. In this study, we examine what effect each of these has in the computation of ocean mass trends using a simulation of ice melting on Greenland, Antarctica, and mountain glaciers. We find that the …

Protein Design By Mining And Sampling An Undirected Graphical Model Of Evolutionary Constraints, John Thomas, Naren Ramakrishnan, Chris Bailey-Kellogg

Computer Science Technical Reports

Evolutionary pressures on proteins to maintain structure and function have constrained their sequences over time and across species. The sequence record thus contains valuable information regarding the acceptable variation and covariation of amino acids in members of a protein family. When designing new members of a protein family, with an eye toward modified or improved stability or functionality, it is incumbent upon a protein engineer to uncover such constraints and design conforming sequences. This paper develops such an approach for protein design: we first mine an undirected probabilistic graphical model of a given protein family, and then use the model …

The Evolution Of Zinjanthropus Boisei, Paul J. Constantino, Bernard A. Wood

Biological Sciences Faculty Research

Many people assume that OH 5, the type specimen of Paranthropus boisei, collected in 1959, was the first evidence of that taxon to be found, but OH 3, recovered in 1955, predated the discovery of OH 5 by four years. Thus, Paranthropus boisei recently celebrated the equivalent of its fiftieth birthday. This review marks that milestone by examining the way our understanding of this taxon has changed during its fifty, or so, year history.

Novel Free Ceramides As Components Of The Soldier Defense Gland Of The Formosan Subterranean Termite (Coptotermes Formosanus), Masaya Ohta, Fumito Matsuura, Gregg Henderson, Roger A. Laine

Faculty Publications

Of the lipid extracts of the defense secretion from the Formosan subterranean termite, Coptotermes formosanus Shiraki, on high-performance thin-layer chromatography analysis, no glycolipids or phospholipids were detected, but free fatty acids and three novel ceramides were found (termed TL-1, TL-2, and TL-3). Free fatty acids were confirmed to be lignoceric acid (C24:0) and hexacosanoic acid (C26:0), as described previously [Chen, J., G. Henderson, and R. A. Laine. 1999. Lignoceric acid and hexacosanoic acid: major components of soldier frontal gland secretions of the Formosan subterranean termite (Coptotermes formosanus). J. Chem. Ecol. 25: 817-824]. TL-1, TL-2, and TL-3 were characterized as ceramides …

Tools For Evaluating Lipolexis Oregmae (Hymenoptera: Aphidiidae) In The Field: Effects Of Host Aphid And Host Plant On Mummy Location And Color Plus Improved Methods For Obtaining Adults, Raghuwinder Singh, Marjorie A. Hoy

Faculty Publications

Lipolexis oregmae Gahan was introduced into Florida in a classical biological control program directed against the brown citrus aphid, Toxoptera citricida (Kirkaldy), on citrus. Prior to evaluating distribution, host range, and potential nontarget effects of L. oregmae in Florida, we evaluated the role of other potential host aphids and host plants on mummy production and location. Under laboratory conditions, this parasitoid produced the most progeny on the target pest, the brown citrus aphid on citrus. This parasitoid, unlike the majority of aphidiids, did not produce mummies on any of the host plants tested when reared in black citrus aphid T. …

Analysis Of A Decade In Library Literature: 1994-2004, Kelly D. Blessinger, Michelle Frasier-Robinson

Faculty Publications

The purpose of this study was to analyze trends in publication and citation in library and information science journals over a decade (1994-2004) of the literature. This examination revealed the areas of concentration within the research, frequently published subjects through the years, and the characteristics of the top-cited authors and resources during this time. This information allows those in the field to follow the trends in publication, gives researchers the tools to determine which journals might give their work the most exposure and recognition, and can help libraries to make collection management decisions in this subject area.

Mobile Element-Based Forensic Genomics, David A. Ray, Jerilyn A. Walker, Mark A. Batzer

Faculty Publications

Mobile elements are commonly referred to as selfish repetitive DNA sequences. However, mobile elements represent a unique and underutilized group of molecular markers. Several of their characteristics make them ideally suited for use as tools in forensic genomic applications. These include their nature as essentially homoplasy-free characters, they are identical by descent, the ancestral state of any insertion is known to be the absence of the element, and many mobile element insertions are lineage specific. In this review, we provide an overview of mobile element biology and describe the application of certain mobile elements, especially the SINEs and other retrotransposons, …

Distribution Of The Hiv Resistance Ccr5-Δ32 Allele Among Egyptians And Syrians, Abdel Halim Salem, Mark A. Batzer

Faculty Publications

A mutant allele of the β-chemokine receptor gene CCR5 bearing a 32-basepair (bp) deletion that prevents cell invasion by the primary transmitting strain of HIV-1 has recently been characterized. Individuals homozygous for the mutation are resistant to infection, even after repeated high-risk exposure, but this resistance appears not absolute, as isolated cases of HIV-positive deletion homozygotes are emerging. The consequence of the heterozygous state is not clear, but it may delay the progression to AIDS in infected individuals. In order to evaluate the frequency distribution of CCR5-Δ32 polymorphism among Egyptians, a total of 200 individuals (154 from Ismailia and 46 …

Production Of F1 Interspecies Hybrid Offspring With Cryopreserved Sperm From A Live-Bearing Fish, The Swordtail Xiphophorus Helleri, Huiping Yang, Leona Hazlewood, Sheila J. Heater, Paula A. Guerrero, Ronald B. Walter, Terrence R. Tiersch

Faculty Publications

Despite study of sperm cryopreservation in more than 200 fish species, production of broods from cryopreserved sperm in live-bearing fish has not been demonstrated. This has not been due to a lack of effort, but instead is a result of the unique morphology, biology, and biochemistry of reproduction in viviparous fishes. For example, sperm of Xiphophorus helleri have a cylindrical nucleus, can swim for days after being activated, have glycolytic capabilities, and can reside in the female reproduction tract for months before fertilization. These traits are not found in fishes with external fertilization. The long-standing research use of the genus …

Early Out-Of-Season Induced Spawning Of Channel Catfish Ictalurus Punctatus (Rafinesque) Conditioned In Heated Earthen Ponds, R. Paul Lang, Terrence R. Tiersch

Faculty Publications

This study documents early out-of-season induced spawning of channel catfish Ictalurus punctatus. During the early spring (February-April) of 1999, 2000 and 2001, ponds containing (1) male and female channel catfish (mixed-sex ponds) or (2) male channel and blue catfish I. furcatus only, or female channel catfish only (single-sex ponds) were heated to 24-30°C to encourage gonadal maturation and spawning. Unheated ponds were stocked with males and females and were monitored during the duration of heating. When natural spawning occurred in the heated ponds, the fish were captured by seining and unspawned females were injected with 100 μg kg-1 of synthetic …

Pediatric Neuroimaging Ethics, Jocelyn Downie, Jennifer Marshall

Articles, Book Chapters, & Popular Press

Neuroimaging has provided insight into numerous neurological disorders in children, such as epilepsy and cerebral palsy. Many clinicians and investigators believe that neuroimaging holds great promise, especially in the areas of behavioral and cognitive disorders. However, concerns about the risks of various neuroimaging modalities and the potential for misinterpretation of imaging results are mounting. Imaging evaluations also raise questions about stigmatization, allocation of resources, and confidentiality. Children are particularly vulnerable in this milieu and require special attention with regards to safety guidelines and modality adaptations. This article examines pediatric neuroimaging practice through an ethics lens. Most authors in the field …

March 1, 2007, University Of North Dakota

University Senate Meeting Minutes

Minutes from the March 1, 2007, meeting of the University Senate. 7 pages

Behavioral And Neurochemical Sources Of Variability Of Circadian Period And Phase: Studies Of Circadian Rhythms Of Npy-/- Mice, Mary E. Harrington, Penny Molyneux, Stephanie Soscia, Cheruba Prabakar, Judy Mckinley-Brewer, Gurprit Lall

Psychology: Faculty Publications

The cycle length or period of the free-running rhythm is a key characteristic of circadian rhythms. In this study we verify prior reports that locomotor activity patterns and running wheel access can alter the circadian period, and we report that these treatments also increase variability of the circadian period between animals. We demonstrate that the loss of a neurochemical, neuropeptide Y (NPY), abolishes these influences and reduces the interindividual variability in clock period. These behavioral and environmental influences, from daily distribution of peak locomotor activity and from access to a running wheel, both act to push the mean circadian period …

Neural Basis Of Stereotype-Induced Shifts In Women's Mental Rotation Performance, Maryjane Wraga, Molly Helt, Emily Jacobs, Kerry Sullivan

Psychology: Faculty Publications

Recent negative focus on women's academic abilities has fueled disputes over gender disparities in the sciences. The controversy derives, in part, from women's relatively poorer performance in aptitude tests, many of which require skills of spatial reasoning. We used functional magnetic imaging to examine the neural structure underlying shifts in women's performance of a spatial reasoning task induced by positive and negative stereotypes. Three groups of participants performed a task involving imagined rotations of the self. Prior to scanning, the positive stereotype group was exposed to a false but plausible stereotype of women's superior perspective-taking abilities; the negative stereotype group …

The Telecommunications Economy And Regulation As Coevolving Complex Adaptive Systems: Implications For Federalism, Barbara A. Cherry

Federal Communications Law Journal

Satisfying the constraints for sustainable regulatory telecommunications policies is more challenging for regulatory regimes based on competition than monopoly. In an earlier paper, Johannes Bauer and I used complexity theory to improve our understanding of the requirements for sustainable telecommunications policies, showing that regulation has a diminishing capacity to achieve specifically desired outcomes and greater attention must be paid to the adaptability of policies and policymaking processes themselves. The present Article examines the implications of the complexity theory perspective for federalism. Federalism is a distinctive (patching) algorithm that confers system advantages for adaptability through diversity and coupling of policymaking jurisdictions-mechanisms …

Transgenic Avian-Derived Recombinant Human Interferon-Alpha2b (Avi-005) In Healthy Subjects: An Open-Label, Single-Dose, Controlled Study., T B Patel, E Pequignot, S H Parker, M C Leavitt, H E Greenberg, Walter K. Kraft

Department of Pharmacology and Experimental Therapeutics Faculty Papers

BACKGROUND/AIMS: This study characterized the safety and pharmacological properties of AVI-005, a novel glycosylated recombinant human interferon-alpha2b produced from the egg whites of chickens transfected with human cDNA.

METHODS: 18 healthy volunteers received single subcutaneous rising doses (0.5, 1.66 or 5 million international units, MIU) of AVI-005. A randomized parallel comparator group of 10 subjects received 5 MIU of unglycosylated IFN-alpha2b (Intron A). The pharmacokinetic parameters t1/2, tmax, Cmax, AUC0-24h, Vd, and clearance were compared between AVI-005 and unglycosylated IFN-alpa2b.

RESULTS: At equipotent doses, AVI-005 had a larger AUC0-24h than the control interferon. Pharmacodynamic markers ofneopterin and beta2-microglobulin for the …

Association Of The Tensin N-Terminal Protein-Tyrosine Phosphatase Domain With The Alpha Isoform Of Protein Phosphatase-1 In Focal Adhesions, Masumi Eto, Jason Kirkbride, Elizabeth Elliott, Su Hao Lo, David L. Brautigan

Department of Molecular Physiology and Biophysics Faculty Papers

Focal adhesions attach cultured cells to the extracellular matrix, and we found endogenous protein phosphatase-1alpha isoform (PP1alpha) localized in adhesions across the entire area of adherent fibroblasts. However, in fibroblasts migrating into a scrape wound or spreading after replating PP1alpha did not appear in adhesions near the leading edge but was recruited into other adhesions coincident in time and space with incorporation of tensin. Endogenous tensin and PP1alpha co-precipitated from cell lysates with isoform-specific PP1 antibodies. Chemical cross-linking of focal adhesion preparations with Lomant's reagent demonstrated molecular proximity of endogenous PP1alpha and tensin, whereas neither focal adhesion kinase nor vinculin …

2007 Abstract Book, Undergraduate Research Forum

Undergraduate Creative Activities and Research Forum Abstract Books

No abstract provided.