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Natural And Anthropogenic Influences On Flow Patterns And Sediment Characteristics In The Dona And Roberts Bay Estuarine System, Sarasota County, Florida, Kyle W. Kelso

USF Tampa Graduate Theses and Dissertations

The Dona and Roberts Bay watershed comprises one of the five major watersheds in Sarasota County, Florida. It is connected to the Gulf of Mexico via Venice Inlet. Like many estuaries in the Florida, significant modifications have been made to the drainage basins, principally to the main tributaries. The system has undergone several anthropogenic modifications to enhance water management efforts. Creeks that comprise fresh water input into the system have been dammed in order to inhibit the upstream flow of salt water during the dry season. They are also deepened or lengthened to allow for better drainage. In addition, biogenic …

Crystallization Studies Of Epigallocatechin Gallate, Sheshanka Kesani

USF Tampa Graduate Theses and Dissertations

Flavonoids are a long and well known class of natural products. Their potential health benefits can be attributed to their antioxidant activity, and modulation of cell signaling pathways. Green tea one of the most widely consumed beverages, consist of flavonoids such as catechins and tannins. Epigallocatechin gallate (EGCG) the major catechin of green tea exhibits multiple health benefits due to its antioxidant nature. The radical scavenging activity of EGCG is attributed to its structure. Therefore, a study on molecular features of EGCG would provide valuable information on structural modifications, which may change the physiochemical properties such as bioavailability and solubility. …

The Impact Of Host Rock Geochemistry On Bacterial Community Structure In Oligotrophic Cave Environments, Hazel A. Barton, Nicholas M. Taylor, Michael P. Kreate, Austin C. Springer, Stuart A. Oehrle, Janet L. Bertog

International Journal of Speleology

Despite extremely starved conditions, caves contain surprisingly diverse microbial communities. Our research is geared toward understanding what ecosystems drivers are responsible for this high diversity. To asses the effect of rock fabric and mineralogy, we carried out a comparative geomicrobiology study within Carlsbad Cavern, New Mexico, USA. Samples were collected from two different geologic locations within the cave: WF1 in the Massive Member of the Capitan Formation and sF88 in the calcareous siltstones of the Yates Formation. We examined the organic content at each location using liquid chromatography mass spectroscopy and analyzed microbial community structure using molecular phylogenetic analyses. In …

2007 Weed Science Research Report – White Section, Ndsu Weed Science, Dept. Of Plant Sciences

Plant Sciences Weed Science Reports

The Weed Science team at North Dakota State University conduct field research in the State of North Dakota. This specific document provides environmental, indexes, abbreviations and collaborators in 2007.

Regulation Of Mdmx Nuclear Import And Degradation By Chk2 And 14-3-3, Cynthia Lebron

USF Tampa Graduate Theses and Dissertations

The MDM2 homolog MDMX is an important regulator of p53 during mouse embryonic development. DNA damage promotes MDMX phosphorylation, nuclear translocation, and degradation by MDM2. Here we show that MDMX copurifies with 14-3-3, and DNA damage stimulates MDMX binding to 14-3-3. Chk2-mediated phosphorylation of MDMX on S367 is important for stimulating 14-3-3 binding, MDMX nuclear import by a cryptic NLS, and degradation by MDM2. Mutation of MDMX S367 inhibits ubiquitination and degradation by MDM2, and prevents MDMX nuclear import. Expression of 14-3-3 stimulates the degradation of phosphorylated MDMX. Chk2 and 14-3-3 cooperatively stimulate MDMX ubiquitination and overcome the inhibition of …

Comparative Effects Of The Toxic Dinoflagellate, Karenia Brevis, On Bivalve Molluscs From Florida, James R. Leverone

USF Tampa Graduate Theses and Dissertations

The effects of the toxic dinoflagellate, karenia brevis (Wilson clone), on larval survival and development of the northern quahog (=hard clam, Mercenaria mercenaria), eastern oyster (Crassostrea virginica) and bay scallop (Argopecten irradians) were studied in the laboratory. The effects of K. brevis on feeding activities of juveniles from these species plus the green mussel (Perna viridis) were also examined. Finally, adult bay scallops were exposed to K. brevis for two weeks to investigate possible cytotoxic effects. Survival of 3-day-old larvae was generally > 85% for all shellfish species at Karenia brevis densities of 100 cells . ml-1 or less, and not …

Faust In Lolita: Composing Sins, Souls, And Rhetorical Redemption, Aurora Mackey

USF Tampa Graduate Theses and Dissertations

Goethe's Faust and Nabokov's Humbert both are erudite, middle-aged European scholars who, experiencing a convergence of academic and existential ennui, set eyes upon a young girl and instantly are consumed with lust. In both works the girls' widowed mothers die as a result of the protagonists' lustful intentions; a cross-country flight ensues; the once-respected scholars are wanted for murder; and Gretchen and Lolita each suffer from their sexual and emotional objectification. But the connections between Goethe's play and Nabokov's novel extend far beyond plot points, or even their decidedly different receptions in early 19th century Germany versus mid-20th century America. …

Cogito Ergo Doceo: A Cross-Case Study Of Latin Teacher Cognition In Technology-Rich Communities Of Practice, Ramã³N Anthony Madrigal

USF Tampa Graduate Theses and Dissertations

This empirical research study investigated the phenomenon of ancient language teacher cognition in the technology-rich contexts of two secondary teachers of Latin. Although one teacher was an inexperienced teacher working in a traditional, face-to-face classroom in an independent school, the other teacher was an experienced teacher working at a public virtual school. Features of teacher cognition, such as pre-active planning and inter-active decision-making were examined from a communities-of-practice theoretical perspective (Wenger, 1998). An exploratory, sequential mixed-methods research design was implemented in this cross-case study. Among the research findings that emerged from this investigation was the notion of the expedient integration …

Using In Situ Click Chemistry To Modulate Protein-Protein Interactions: Bcl-XL As A Case, Lisa M. Malmgren

USF Tampa Graduate Theses and Dissertations

Protein-protein interactions are central to most biological processes. Although in the field of drug discovery there is a great interest in targeting protein-protein interactions, the discovery and development of small-molecules, which effect these interactions has been challenging. The purpose of this project is to determine if in situ click chemistry is a practical approach towards testing whether Bcl-XL is capable of assembling it's own inhibitory compounds. Abbott laboratories developed compound ABT-737, which binds with high affinity (Ki < 1 nM) to the binding sites of Bcl-XL.³⁶. Based on ABT-737, two acetylene anchor molecules AM3 and AM4 have been synthesized. These anchor molecules are distinguished by the reactivity …

A Comparative Ecological Study Between Coyotes (Canis Latrans) In A Protected And Urban Habitat: A Closer Look At Enteric Parasites And Diet Between Florida Coyotes, Denara Lynn Manning

USF Tampa Graduate Theses and Dissertations

Coyotes (Canis latrans) have inhabited Florida (USA) since the 1960s and are currently found throughout the state. The purpose of the present study was to obtain information on enteric parasites and diet of Florida coyotes from two different habitat types. Seasonal variation in diet was also examined. Fresh coyote fecal samples were collected from protected and urban habitats in Pinellas County, Florida (USA; 27°54' n, 82°41'w) from may 2005 to march 2007. A standard fecal flotation examination and formalin-ethyl acetate sedimentation were utilized on fecal samples collected from the protected (n=40) and urban (n=50) habitats. Five novel (newly documented) parasites …

Transient Fluorescence Spectroscopy And Laser Induced Fluorescence Lifetimes Of Terbium Doped Dipicolinic Acid, Anali Makoui

USF Tampa Graduate Theses and Dissertations

We have investigated the use of deep UV laser induced fluorescence for the sensitive detection and spectroscopic lifetime studies of terbium doped dipicolinic acid (DPA-Tb) and used this to study the optical characteristics of DPA which is a chemical surrounding most bacterial spores. Background absorption spectra, fluorescence spectra, and Excitation Emission Matrix (EEM) spectra were made of the DPA-Tb complex, using both fixed 266 nm wavelength and tunable (220 nm --

280 nm) UV laser excitations. Of importance, the fluorescence lifetimes of the four main fluorescence peaks (488 nm, 543 nm, 581 nm, and 618 nm) of the DPA-Tb complex …

On The Theory Of Records And Applications, Alfred Kubong Mbah

USF Tampa Graduate Theses and Dissertations

No abstract provided.

The Effect Of Optional Real World Application Projects On Mathematics Achievement Among Undergraduate Students, David Milligan

USF Tampa Graduate Theses and Dissertations

Many undergraduate students enrolled in institutions of higher learning wish to connect their learning to real life experiences. By linking reality to academics, students see first hand the practical value in their studies. The purpose of this study was to critically analyze the practice of application projects in undergraduate mathematics courses to determine if, and if so how, students benefit from optional real world application projects. The study was limited to specific courses within a non-math major's undergraduate mathematics program of study at one large research university. Until the appearance of "The Mathematics Umbrella: Modeling and Education" (Grinshpan, 2005), no …

Between Knowledge & Practice: Factors That Influence The Operationalization Of Sexual Health Knowledge In African American Female College Students, Jamae F. Morris

USF Tampa Graduate Theses and Dissertations

This paper explores some of the factors that influence whether African American female college students implement safe sex knowledge. High rates of HIV/AIDS and other sexually-transmitted infections (STIs) in the black community and the overrepresentation of individuals under the age of 25 in sexually-transmitted infection (STI) reports indicate the need for research that investigates the physical, social, and cultural aspects of high-risk sexual behaviors and the factors that influence them for this group. African American female college students present a unique challenge to sexual health educators; a challenge that has been relatively under acknowledged and under addressed in research and …

Molecular And Genomic Studies Of Temperate Phages From Halomonas Aquamarina And Bacillus Spp. Isolates From The Gulf Of Mexico, Jennifer M. Mobberley

USF Tampa Graduate Theses and Dissertations

Viruses are the most abundant biological entities in the ocean and are believed to contribute to nutrient cycling, bacterial diversity, and horizontal gene exchange. However, little is known about the relationship between temperate phages and their hosts in marine environments. In this thesis, phage-host systems from the Gulf of Mexico were used to study the influence of temperate phages in bacteria. PhiHAP-1 is a temperate myovirus induced with mitomycin C from Halomonas aquamarina isolate. The genome of this phage was 39,245 nucleotides long and contained 46 predicted genes. Besides genes involved in lysogeny, PhiHAP-1 contained a protelomerase, which is responsible …

Self Assembly Of Complex Structures, Michael Nellis

USF Tampa Graduate Theses and Dissertations

The state of the art in artificial micro self assembly concepts are reviewed. The history of assembly is presented with a comparison to macro assembly, which has been widely studied, and micro self assembly. Criteria were developed and tested to show that macro assembly is more complex in ways that micro self assembly is not. Self assembly requirements for successful and complex self assembly, which evolved from the macro and micro comparison, are also established and tested. A method to assemble complex structures in the micro scale is proposed and demonstrated at the meso scale. The basic concepts of self …

A Three-Dimensional Biophysical Model Of Light, Nutrient, And Grazing Controls On Phytoplankton Competition Affecting Red Tide Maintenance On The West Florida Shel, Scott P. Milroy

USF Tampa Graduate Theses and Dissertations

A coupled, three-dimensional, time-dependent numerical model of water circulation, spectral light, plankton dynamics, nutrient/CDOM loadings, and zooplankton grazing provided an assessment of the factors affecting the growth and maintenance of red tides on the west Florida shelf (WFS). The coupled biophysical model consisted of state variable quantities for temperature, salinity, horizontal/vertical velocity components, turbulent diffusion, spectral light, colored dissolved organic matter (CDOM), dissolved organic and inorganic carbon, particulate silica, four dissolved inorganic nutrient pools (nitrate, ammonium, phosphate, and silicate), and four phytoplankton groups (diatoms, microflagellates, non-toxic dinoflagellates, and the red tide organism Karenia brevis).

The model also included a complex …

Akt Function And Human Oncogenesis, Sungman Park

USF Tampa Graduate Theses and Dissertations

Accumulated evidence indicates that, by the phosphorylation of its physiological substrates, Akt promotes cell survival, proliferation and angiogenesis. While a number of Akt targets have been identified, the mechanism by which Akt regulates cell survival and growth and induces malignant transformation still remains elusive. During the last 5 years, I have shown that AKT1 cross-talks with Src/Stat3 pathway. AKT1 is a direct target gene of Stat3. Protein/mRNA levels and promoter activity of AKT1 are significantly induced by constitutively active Src and Stat3. Knockdown of Stat3 or dominant-negative Stat3 reduced AKT1 expression induced by constitutively active Src. Blockage of AKT1 expression …

An Existential-Phenomenological Investigation Of Self-Cutting Among Adolescent Girls, Amanda A. Privé

USF Tampa Graduate Theses and Dissertations

The present study examined the experience of self-cutting, what stops adolescent girls from engaging in self-cutting, and what message adolescent girls who are self-cutting would want to send to other girls taking part in this behavior. Using an existential-phenomenological method of interviewing, adolescent girls were asked a serious of questions in order to gain insight into their thoughts, feelings, and beliefs about the experience of self-cutting. Each interview was tape recorded, transcribed, and thematized. The participants in this study were labeled co-researchers due to the significant role that they had in the completion of this study. The co-researchers consisted of …

Modifiable Factors For Disability: Is There Potential For Reducing Racial Disparities In Disability In Older Age?, Mihaela A. Popa

USF Tampa Graduate Theses and Dissertations

White-Black disparities in disability in the older population are consistently reported in the literature, and are usually ascribed to differences in socio-economic, health, and cognitive status. However, the role of modifiable influences on disability, such as health and lifestyle behaviors or health care utilization on these differences is less clear. This dissertation examines: (1) longitudinal White-Black differences in disability and the potential contributions of distinct health behaviors to these differences; (2) White-Black differences in the effects of health care utilization on trajectories of disability; and (3) whether cognition mediates the effects of health behaviors on disability in Whites and in …

Real Challenges, Virtual Challengers: The Democracy For America Movement, Noah Porter

USF Tampa Graduate Theses and Dissertations

The project examines the effects of Internet use by the social movement organization (SMO) Dean for America/Democracy for America (DFA). This study describes the relationship between Internet usage and SMO beliefs, organization, causes, reasons for joining, strategies, reactions, and effects. Dean for America started out with few resources or supporters, and was seen as an unlikely contender for the 2004 presidential campaign. From its humble beginnings, it skyrocketed from being virtually unknown to frontrunner status in the span of a year. After Howard Dean withdrew from the race in February 2004, Dean for America transformed itself into Democracy for America, …

A 1400-Year Multi-Proxy Record Of Climate Variability From The Northern Gulf Of Mexico, Julie N. Richey

USF Tampa Graduate Theses and Dissertations

A continuous, decadal-scale resolution multi-proxy record of climate variability over the past 1400 years in the northern Gulf of Mexico (GOM) was constructed from a box core recovered in the Pigmy Basin. Proxies include paired analyses of Mg/Ca and oxygen isotopes in the white variety of the planktic foraminifer Globigerinoides ruber and relative abundance variations of G. sacculifer in the foraminifer assemblages. Two multi-decadal intervals of sustained high Mg/Ca values indicate GOM sea-surface temperatures (SSTs) were as warm or warmer than near-modern conditions between 1000 and 1400 yrs BP. Foraminiferal Mg/Ca values during the coolest interval of the Little Ice …

The Influence Of The Loop Current On The Diversity, Abundance And Distribution Of Zooplankton In The Gulf Of Mexico, Katie Rathmell

USF Tampa Graduate Theses and Dissertations

Physical processes in the Gulf of Mexico (GOM) and mesoscale (10-300 km) processes associated with the Loop Current are fairly well known. However, little is known about the physical/ biological interactions of the frontal boundary system of the Loop Current. Zooplankton abundance and distribution was determined at 28 stations in the vicinity of the Loop Current. Species richness was high at all stations. Copepods comprised 60% of the total zooplankton collected. Oithona plumifera, Nannocalanus minor and Euchaeta marina were the most abundant copepods. Chaetognaths and ostracods were also very abundant and made up 11 and 5 % respectively of the …

Relationship Between Nurses’ Management Of Pediatric Oncology Patients’ Symptoms And Job Satisfaction, Jennifer I. Rheingans

USF Tampa Graduate Theses and Dissertations

A primary function of the pediatric oncology nurse is to provide symptom management to children with cancer. Symptom management strategies have been published, but there is scarce literature examining neither the actual use of these nursing interventions, nor the effects of using these interventions on the nurses' perceived work environment. The purpose of this study was to examine the nursing interventions used in treating pediatric oncology patients' symptoms, as well as the emotional sequelae from providing this care. Phase One of this study examined the content validity of the newly developed Nurses Distress and Interventions for Symptoms Survey (NDISS) utilizing …

Perceptions Of Senior Faculty Concerning Doctoral Student Preparation For Faculty Roles, Jennifer M. Purcell

USF Tampa Graduate Theses and Dissertations

Calls for reform in doctoral education are not new. However, the past decade has experienced renewed interest and discussion in preparing the future professoriate. Whereas most studies of graduate student socialization and preparation for faculty roles have focused on doctoral students or new faculty, this study examined the perceptions of senior faculty members involved in doctoral education. All senior faculty (n=4970) in biological sciences, English, mathematics, and non-clinical psychology from a stratified sample of 69 research universities nationwide were invited to participate. More than 1150 faculty completed a web-based survey. Respondents rated the importance of 18 competencies (based on a …

Determining Habitat Preferences Of The Juvenile Gopher Tortoise (Gopherus Polyphemus) Using Spatially Modeled Vegetation On A Central Florida Sandhill, Kristan Marie Nicole Raymond

USF Tampa Graduate Theses and Dissertations

Public and private conservation areas are becoming increasingly important to the continued survival of the gopher tortoise, making it imperative that land managers know the specific habitat requirements of juvenile gopher tortoises because recruitment is key to species persistence. Little is currently known about environmental factors that underlie hatchling and juvenile survival and recruitment in gopher tortoise populations. Because of the short duration and distance of juvenile tortoise foraging journeys, food availability, thermoregulatory conditions, and refugia near the burrow may considerably affect juvenile growth and survival. This two-year study of a central Florida sandhill examines the spatial relationship between juvenile …

Bcl-2 Related Ovarian Killer, Bok, Is Cell Cycle Regulated And Sensitizes To Stress-Induced Apoptosis, José M. Rodríguez

USF Tampa Graduate Theses and Dissertations

Bok/Mtd (Bcl-2-related ovarian killer/Matador) is considered a pro-apoptotic member of the Bcl-2 family. Though identified in 1997, little is known about its biological role. We have previously demonstrated that Bok mRNA is upregulated following E2F1 over-expression. In the current work, we demonstrate that Bok RNA is low in quiescent cells and rises upon serum stimulation. To determine the mechanism underlying this regulation, we cloned and characterized the mouse Bok promoter. We find that the mouse promoter contains a conserved E2F binding site (-43 to -49) and that a Bok promoter-driven luciferase reporter is activated by serum stimulation dependent on this …

Credibility Perceptions Of Television And Online News, Charmy G. Sabigan

USF Tampa Graduate Theses and Dissertations

Three major factors influence audience's credibility perception of mediated news on television and on the internet. This study found that reporters' credibility, media credibility, and news credibility had direct influence to the credibility of news presented on both media. Reporters' credibility on both media could be measured by their expertise, intelligence, education, trustworthiness, and authoritativeness. Television and the internet were evaluated differently. Television was measured by its comprehensiveness, concern for the interest of the public, and fairness. The internet was assessed on its trustworthiness, consideration of public interest, and objectivity. News credibility for both media, however, could be evaluated using …

An Experimental Study Of Pro-Dieting And Anti-Dieting Psychoeducational Messages: Effects On Immediate And Short-Term Psychological Functioning And Weight Control Practices In College Women, Megan Roehrig

USF Tampa Graduate Theses and Dissertations

While dieting is relatively normative in our society, it is controversial within the fields of eating disorders and obesity. Dieting for weight loss has been touted by the obesity prevention field as a solution to the growing obesity epidemic, yet a body of research in the eating disorders field has also implicated it in the etiology and maintenance of eating pathology. Thus, a divergence in approaches toward dieting has emerged, with both prodieting and anti-dieting messages being recommended. Little is known, however, about the impact of these two types of messages on immediate and short-term psychological functioning and weight control …

Synthesis And Characterization Of Interfaces Between Naturally Derived And Synthetic Nanostructures For Biomedical Applications, Souheil Zekri

USF Tampa Graduate Theses and Dissertations

The use of nanotechnology to develop methods for fabrication and characterization of organized hybrid nanostructures that include integrated polymeric, biological and inorganic compounds has increased exponentially during the last decade. Such bio-nano-composite materials could be used in solving current biomedical problems spanning from nanomedicine to tissue engineering and biosensing. In this dissertation, a systematic study has been carried out on the synthesis, characterization, of two interfaces between naturally derived and synthetic nanostructures. Carbon nanotubes and porous silicon represent the synthetic nanostructures that were developed for the purpose of interfacing with the naturally derived bovine type I collagen and respiratory syncytial …