Digital Commons Network^™

Georgeoral Immunization Of Rhesus Macaques With Adenoviral Hiv Vaccines Using Enteric-Coated Capsules, George T. Mercier, Pramod N. Nehete, Marco F. Passeri, Bharti N. Nehete, Eric A. Weaver, Nancy Smyth Templeton, Kimberly Schluns, Stephanie S. Buchl, K. Jagannadha Sastry, Michael A. Barry

Nebraska Center for Virology: Faculty Publications

Targeted delivery of vaccine candidates to the gastrointestinal (GI) tract holds potential for mucosal immunization, particularly against mucosal pathogens like the human immunodeficiency virus (HIV). Among the different strategies for achieving targeted release in the GI tract, namely the small intestine, pH sensitive enteric coating polymers have been shown to protect solid oral dosage forms from the harsh digestive environment of the stomach and dissolve relatively rapidly in the small intestine by taking advantage of the luminal pH gradient. We developed an enteric polymethacrylate formulation for coating hydroxy-propyl-methyl-cellulose (HPMC) capsules containing lyophilized Adenoviral type 5 (Ad5) vectors expressing HIV-1 gag …

Survey Of Selected Pathogens And Blood Parameters Of Northern Yellowstone Elk: Wolf Sanitation Effect Implications, Shannon M. Barber-Meyer, P. J. White, L. David Mech

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

The restoration or conservation of predators could reduce seroprevalences of certain diseases in prey if predation selectively removes animals exhibiting clinical signs. We assessed disease seroprevalences and blood parameters of 115 adult female elk (Cervus elaphus) wintering on the northern range of Yellowstone National Park [YNP] during 2000– 2005 and compared them to data collected prior to wolf (Canis lupus) restoration (WR) in 1995 and to two other herds in Montana to assess this prediction. Blood parameters were generally within two standard deviations of the means observed in other Montana herds (Gravelly-Snowcrest [GS] and Garnet Mountain …

Differential Engagement Of Tim-1 During Activation Can Positively Or Negatively Costimulate T Cell Expansion And Effector Function, Vijay K. Kuchroo, Sheng Xiao, Nader Najafian, Jay Reddy, Monica Albin, Chen Zhu, Eric Jensen, Jaimie Imitola, Thomas Korn, Ana C. Anderson, Zheng Zhang, Cristina Gutierrez, Thomas Moll, Raymond A. Sobel, Dale T. Umetsu, Hideo Yagita, Hisaya Akiba, Terry Strom, Mohamed H. Sayegh, Rosemarie H. Dekruyff, Samia J. Khoury

School of Veterinary and Biomedical Sciences: Faculty Publications

It has been suggested that T cell immunoglobulin mucin (Tim)-1 expressed on T cells serves to positively costimulate T cell responses. However, crosslinking of Tim-1 by its ligand Tim-4 resulted in either activation or inhibition of T cell responses, thus raising the issue of whether Tim-1 can have a dual function as a costimulator. To resolve this issue, we tested a series of monoclonal antibodies specifi c for Tim-1 and identifi ed two antibodies that showed opposite functional effects. One anti–Tim-1 antibody increased the frequency of antigen-specifi c T cells, the production of the proinfl ammatory cytokines IFN-γ and IL-17, …

Modulation Of Cd4 Co-Receptor Limits Spontaneous Autoimmunity When High-Affinity Transgenic Tcr Specific For Self-Antigen Is Expressed On A Genetically Resistant Background, Zsolt Illés, Hanspeter Waldner, Jay Reddy, Ana C. Anderson, Raymond A. Sobel, Vijay K. Kuchroo

Jay Reddy Publications

Myelin proteolipid protein (PLP) 139–151 is an immunodominant peptide that induces experimental autoimmune encephalomyelitis (EAE) in H-2^s SJL/J mice. While PLP 139–151-specific TCR transgenic (tg) 4E3 mice develop fulminant spontaneous disease on the susceptible SJL/J background, spontaneous EAE is dramatically reduced on the H-2^s congenic B10.S background. On this resistant background, we observed a high frequency of positively selected tg CD42CD82 (DN) thymocytes and peripheral DN tg T cells. Splenic DN tg T cells responded to anti-CD3 stimulation similarly to CD41 cells, but proliferative and cytokine responses to PLP 139–151 were blunted, implying that CD4 coreceptor down-regulation modulated …

Species-Specific Visitation And Removal Of Baits For Delivery Of Pharmaceuticals To Feral Swine, Tyler A. Campbell, David B. Long

United States Department of Agriculture Wildlife Services: Staff Publications

Within the domestic swine industry there is growing trepidation about the role feral swine (Sus scrofa) play in the maintenance and transmission of important swine diseases. Innovative disease management tools for feral swine are needed. We used field trials conducted in southern Texas from February to March 2006 to compare species-specific visitation and removal rates of fish-flavored and vegetable-flavored baits with and without commercially available raccoon (Procyon lotor) repellent (trial 1) and removal rates of baits deployed in a systematic and cluster arrangement (trial 2). During trial 1, 1) cumulative bait removal rates after four nights …

Review Of Infectious Diseases Of Wild Rodents By Dennis Padovan, Robert G. Mclean

United States Department of Agriculture Wildlife Services: Staff Publications

Infectious Diseases of Wild Rodents is a collection of reference material on a wide variety of infectious diseases documented in rodent species throughout the world. Editorially, this was a massive undertaking to provide a comprehensive and systematic coverage of the information on so many rodent species and infectious diseases in one volume. Although the information is not presented in much detail, the breadth of coverage makes this a good reference book for those interested in rodent diseases throughout the world. The information is presented in two different formats: disease information in the first part of the book is arranged by …

Protection With Recombinant Clostridium Botulinum C1 And D Binding Domain Subunit (Hc) Vaccines Against C And D Neurotoxins, Robert P. Webb, Theresa J. Smith, Patrick M. Wright, Vicki A. Montgomery, Michael M. Meagher, Leonard A. Smith

Michael Meagher Publications

Recombinant botulinum Hc (rBoNT Hc) vaccines for serotypes C1 and D were produced in the yeast Pichia pastoris and used to determine protection against four distinct BoNT C and D toxin subtypes. Mice were vaccinated with rBoNT/C1 Hc, rBoNT/D Hc, or with a combination of both vaccines and challenged with BoNT C1, D, C/D, or D/C toxin. Mice receiving monovalent vaccinations were partially or completely protected against homologous toxin and not protected against heterologous toxin. Bivalent vaccine candidates completely survived challenges from all toxins except D/C toxin. These results indicate the recombinant C1 and D Hc vaccines are not only …

Genetic Microheterogeneity And Phenotypic Variation Of Helicobacter Pylori Arginase In Clinical Isolates, Justin Hovey, Emily Watson, Melanie Langford, Ellen Hildebrandt, Sangeetha Bathala, Jeffrey Bolland, Domenico Spadafora, George Mendz, David Mcgee

School of Biological Sciences: Faculty Publications

Background: Clinical isolates of the gastric pathogen Helicobacter pylori display a high level of genetic macro- and microheterogeneity, featuring a panmictic, rather than clonal structure. The ability of H. pylori to survive the stomach acid is due, in part, to the arginase-urease enzyme system. Arginase (RocF) hydrolyzes L-arginine to L-ornithine and urea, and urease hydrolyzes urea to carbon dioxide and ammonium, which can neutralize acid.

Results: The degree of variation in arginase was explored at the DNA sequence, enzyme activity and protein expression levels. To this end, arginase activity was measured from 73 minimally-passaged clinical isolates and six laboratory-adapted strains …

Asymmetric Localizations Of Lin-17/Fz And Mig-5/Dsh Are Involved In The Asymmetric B Cell Division In C. Elegans, Mingfu Wu, Michael A. Herman

School of Biological Sciences: Faculty Publications

LIN-44/Wnt and LIN-17/Frizzled (Fz) function in a planar cell polarity (PCP)-like pathway to regulate the asymmetric B cell division in Caenorhabditis elegans. We observed asymmetric localization of LIN-17/Frizzled (Fz) and MIG-5/Disheveled (Dsh) during the B cell division. LIN-17∷GFP was asymmetrically localized within the B cell prior to and after the B cell division and correlated with B cell polarity. Asymmetric localization of LIN-17∷GFP was dependent upon LIN-44/Wnt and MIG-5/Dsh function. The LIN-17 transmembrane domain and a portion of the cysteine-rich domain (CRD) were required for LIN-17 function and asymmetric distribution to the B cell daughters, while the conserved KTXXXW …

First Observation Of Elaeophora Schneideri Wehr And Dikmans, 1935 (Nematoda:Filariidae) In Mule Deer From Nebraska, R. D. Mcknown, M. C. Sterner, D. W. Oates

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Between November 2000 and November 2005, approximately 200 mule deer (Odocoileus hemionus hemionus) and whitetailed deer (Odocoileus virginianus) from western Nebraska were extensively examined for the presence of Elaeophora schneideri, Wehr and Dikmans, 1935; three adult E. schneideri were detected from three mule deer. This represents the first documented occurrence of E. schneideri from wild deer in Nebraska.

Parasitism Of Hylodes Phyllodes (Anura: Cycloramphidae) By Hannemania Sp. (Acari: Trombiculidae) In An Area Of Atlantic Forest, Ilha Grande, Southeastern Brazil, F. H. Hatano, Donald Gettinger, M. Van Sluys, C. F. D. Rocha

Harold W. Manter Laboratory of Parasitology: Faculty Publications

We studied some parameters of the parasitism by the mite Hannemania sp. on the endemic frog Hylodes phyllodes in the Atlantic Forest of Ilha Grande (Rio de Janeiro State, Brazil). Prevalence, mean abundance, mean intensity and total intensity of infestation, body regions infected, and host sexual differences in parasitism rate of the larvae of Hannemania sp. on individuals of H. phyllodes were assessed. Prevalence was 86.5 % (N = 37; total of 1,745 larvae of Hannemania sp) for male hosts and 91.7 % (N =12; total of 330 larvae) for female hosts, with no significant difference between the sexes. Overall …

Protostrongylid Parasites And Pneumonia In Captive And Wild Thinhorn Sheep (Ovis Dalli), Emily J. Jenkins, A. M. Veitch, Susan J. Kutz, T. K. Bollinger, J. M. Chirino-Trejo, B. T. Elkin, K. H. West, Eric P. Hoberg, L. Polley

Harold W. Manter Laboratory of Parasitology: Faculty Publications

We describe health significance of protostrongylid parasites (Parelaphostrongylus odocoilei and Protostrongylus stilesi) and other respiratory pathogens in more than 50 naturally infected Dall’s sheep (Ovis dalli dalli) from the Mackenzie Mountains, Northwest Territories (1998–2002) as well as in three Stone’s sheep (O. d. stonei) experimentally infected with P. odocoilei (2000–2002). Histological lesions in the brain and distribution of P. odocoilei in the muscles of experimentally and naturally infected sheep were consistent with a previously hypothesized ‘‘central nervous system to muscle’’ pattern of migration for P. odocoilei. Dimensions of granulomas associated with eggs of …

Eastward Ho: Phylogeographical Perspectives On Colonization Of Hosts And Parasites Across The Beringian Nexus [Guest Editorial], Eric Waltari, Eric P. Hoberg, Enrique P. Lessa, Joseph A. Cook

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The response of Arctic organisms and their parasites to dramatic fluctuations in climate during the Pleistocene has direct implications for predicting the impact of current climate change in the North. An increasing number of phylogeographical studies in the Arctic have laid a framework for testing hypotheses concerning the impact of shifting environmental conditions on transcontinental movement. We review 35 phylogeographical studies of trans-Beringian terrestrial and freshwater taxa, both hosts and parasites, to identify generalized patterns regarding the number, direction and timing of trans-continental colonizations. We found that colonization across Beringia was primarily from Asia to North America, with many events …

Antenatal Risk Factors, Cytokines And The Development Of Atopic Disease In Early Childhood, Esther K. Chung, Rachel L. Miller, Michael T. Wilson, Stephen J. Mcgeady, Jennifer F. Culhane

Department of Pediatrics Faculty Papers

Atopic diseases are complex entities influenced by an array of risk factors including genetic predisposition, environmental allergens, antenatal exposures, infections and psychosocial factors. One proposed mechanism by which these risk factors contribute to the development of atopic disease is through alterations in the production of T helper type 1 (Th1) and type 2 (Th2) cytokines. The objectives of this review are to discuss antenatal exposures that are associated with pediatric atopic diseases, to discuss the influence of the intrauterine environment on neonatal immune responses, to provide an overview of the Th1 and Th2 pathways and how they relate to atopic …

Chlorophacinone Baiting For Belding’S Ground Squirrels, Craig A. Ramey, George H. Matschke, Richard M. Engeman

United States Department of Agriculture Wildlife Services: Staff Publications

The efficacy of using 0.01% chlorophacinone on steam-rolled oat (SRO) groats applied in CA alfalfa by spot-baiting/hand baiting around burrow entrances (~11.5 g) to control free-ranging Belding's ground squirrels (Spermophilus beldingi) were compared in 6 randomly assigned square treatment units (TUs). Four TUs were given the rodenticide and 2 treated with placebo bait. Each TU was a 0.4 ha square surrounded by a similarly treated 5.5 ha square buffer zone. Baits were applied on May 13 and re-applied, on May 20 and May 22, after 7 days of un-forecasted cool wet weather greatly reduced their above ground activity. Pesticide (EPA …

Prevalence Of Extended-Spectrum Beta-Lactamases-Producing Isolates Over A 1-Year Period At A University Hospital In Oman, Akbar M. Rafay, Zakariya Al-Muharrmi, Robert Toki

Saudi Medical Journal

OBJECTIVE: To evaluate the prevalence of extended-spectrum beta-lactamases isolates over one year period at Sultan Qaboos University Hospital. METHODS: We identified the ESBL isolates during a 12-month period from July 2004 to June 2005, using a commercial system, and confirmed the result using the National Committee for Clinical Laboratory Standards-approved double-disk diffusion method. RESULTS: Sensitivity was recorded for a wide range of antibiotics, aminoglycosides, carbapenem, cephalosporins, quinolones, aztreonam, ampicillin, amoxicillin/clavulanate, ampicillin/sulbactam, piperacillin-tazobactam, trimethoprim/ sulfamethoxazole and nitrofurantoin. Of the total ESBL isolated, 29.6% were from medical ward, followed by outpatients clinic, 24.3%. Urine was the main source of ESBLs 70.4%, followed …

Causes Of Nosocomial Pneumonia And Evaluation Of Risk Factors In A University Hospital In Turkey, Lutfu Savas, Yusuf Onlen, Nizami Duran, Nazan Savas

Saudi Medical Journal

OBJECTIVE: To determine the incidence, risk factors, mortality rate, and organisms causing nosocomial pneumonia (NP). METHODS: We carried out this study in the Intensive Care Units (ICUs), General Medical and Surgical wards of Baskent University, Training and Research Hospital in Adana, Turkey, between November 2000 and August 2002. Patients were observed from the time of admission until 48 hours after discharge from the hospital. RESULTS: In this study, 618 (2.1%) nosocomial infections (NIs) were detected in 29778 patients. One hundred and fifteen of these infections were NP and investigated with surveillance prospectively. The most frequently isolated microorganisms in NP were …

Bk Virus Infection In A Renal Transplant Saudi Child, Mohamed Maghrabi, Dalia Marwan, Abimbola O. Osoba

Saudi Medical Journal

BK human polyomavirus BKV causes an asymptomatic primary infection in children, but later, establishes latency mainly in the urinary tract. Virus-host interactions influencing persistence and pathogenicity are not well-understood. We present here a 12-year-old Saudi boy, who had renal transplant in Egypt. Seven months later, he was admitted to our Pediatric Nephrology Unit as a case of renal impairment. He developed BKV infection, diagnosed and successfully managed in our hospital. This case demonstrates the expanding clinical importance of BKV in a post renal transplant patient. This virus can be detected in transitional cells in the urine decoy cells using cytology. …

Parasites, Proteomics And Performance: Effects Of Gregarine Gut Parasites On Dragonfly Flight Muscle Composition And Function, Rudolf J. Schilder, James H. Marden

School of Biological Sciences: Faculty Publications

In previous work, we found that dragonflies infected with gregarine gut parasites have reduced muscle power output, loss of lipid oxidation in their flight muscles, and a suite of symptoms similar to mammalian metabolic syndrome. Here, we test the hypothesis that changes in muscle protein composition underlie the observed changes in contractile performance. We found that gregarine infection was associated with a 10-fold average reduction in abundance of a ~155·kDa fragment of muscle myosin heavy chain (MHC; ~206·kDa intact size). Insect MHC gene sequences contain evolutionarily conserved amino acid motifs predicted for calpain cleavage, and we found that calpain digestion …

Grânulos De Amido E Fitólitos Em Cálculos Dentários Humanos: Contribuição Ao Estudo Do Modo De Vida E Subsistência De Grupos Sambaquianos Do Litoral Sul Do Brasil, Verônica Wesolowski, Sheila Maria Ferraz Mendonça De Souza, Karl Reinhard, Gregório Ceccantini

Karl Reinhard Publications

Este artigo tem por objetivo apresentar os resultados da recuperação e análise de micro-vestígios vegetais retidos em cálculos dentários de grupos de pescadores-coletores do litoral sul do Brasil. Apresenta também os resultados das relações mantidas entre grânulos de amido e cárie, e entre fitólitos e desgaste dentário.

Através da dissolução química de cálculos dentários foram recuperados microvestígios vegetais em todos os indivíduos estudados. Foi evidenciado o consumo de alimentos amiláceos em todas as séries esqueléticas estudadas, demonstrando independência entre dieta com aporte de produtos vegetais e utilização de cerâmica. Foram encontrados resultados sugestivos de padrões de escolha de alimentos diferenciados …

How Will Global Climate Change Affect Parasite-Host Assemblages?, Eric P. Hoberg, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Parasites are integral components of the biosphere. Host switching correlated with events of episodic climate change is ubiquitous in evolutionary and ecological time. Global climate change produces ecological perturbations, which cause geographical and phenological shifts, and alteration in the dynamics of parasite transmission, increasing the potential for host switching. The intersection of climate change with evolutionary conservative aspects of host specificity and transmission dynamics, called ecological fitting, permits emergence of parasites and diseases without evolutionary changes in their capacity for host utilization.

Nearctic Shrews, Sorex Spp., As Paratenic Hosts Of Soboliphyme Baturini (Nematoda: Soboliphymidae), S. V. Karpenko, N. E. Dokuchaev, Eric P. Hoberg

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Third-stage larvae (L3) of Soboliphyme baturini were discovered for the first time in shrews, Sorex cinereus and Sorex tundrensis from Alaska and the Nearctic. Shrews were found to be infected with L3 at Suloia Lake, southeastern Alaska, Togiak National Wildlife Refuge, southwestern Alaska, and at the Yukon-Charley Rivers National Preserve near the Yukon-Alaska border. Larvae in shrews from Alaska were morphologically indistinguishable from those known in both insectivores and arvicoline rodents from Russia. The occurrence of S. baturini in Alaskan insectivores further establishes shrews as important hosts in the transmission of S. baturini among mustelids and other carnivores and indicates …

First Record Of Alcataenia Larina Larina (Cestoda: Dilepididae) In Atlantic Puffins (Aves, Alcidae, Fratercula Arctica) From Newfoundland, Canada, Sabir B. Muzaffar, Eric P. Hoberg, Ian L. Jones

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The tapeworm Alcataenia larina (Cestoda: Cyclophyllidea: Dilepididae) is a parasite of gulls (Laridae) with a Holarctic distribution in oceanic and littoral habitats. Two subspecies Alcataenia larina pacifica and Alcataenia larina larina are recognized with the former occurring in the North Pacific basin and the latter in the North Atlantic. Alcids serve as incidental hosts for both species and infections are generally rare, usually involving few specimens. We report A. l. larina in Atlantic puffins (Fratercula arctica) collected from the Bay of Exploits, Newfoundland. The 14 strobilate and early gravid specimens were clearly distinguishable from the closely related Alcataenia …

Serologic Evidence Of West Nile Virus Exposure In North American Mesopredators, Kevin T. Bentler, Jeffrey S. Hall, J . Jeffrey Root, Kaci Klenk, Brandon S. Schmit, Bradley F. Blackwell, Paul C. Ramey, Larry Clark

United States Department of Agriculture Wildlife Services: Staff Publications

Sera from 936 mammalian mesopredators (Virginia opossums, gray foxes, striped skunks, hooded skunks, raccoons, a bobcat, and a red fox) were collected during 2003 and 2004 in California, Arizona, Texas, Louisiana, Ohio, and Wyoming and screened for flavivirus-specific antibodies by an epitope-blocking enzyme-linked immunosobent assay (blocking ELISA). Serum samples positive for antibodies against flaviviruses were screened for West Nile virus (WNV)– specific antibodies by blocking ELISA and selectively confirmed with plaque-reduction neutralization tests. High prevalence rates were observed in raccoons (45.6%) and striped skunks (62.9%). The high WNV antibody prevalence noted in mesopredators, their peridomestic tendencies, and their overall pervasiveness …

Current And Historical Use Of Alpha-Chloralose On Wild Turkeys, David Bergman, Brian F. Wakeling, Timothy B. Veenendaal, John D. Eisemann, Thomas W. Seamans

United States Department of Agriculture Wildlife Services: Staff Publications

Alpha-chloralose (AC) has been used as an anesthetic since 1897 to capture or sedate wildlife, including waterfowl, wood-pigeon (Columba palumbus), and black bear (Ursus americana). The first use of AC in the United States was for the capture of house sparrows (Passer domesticus), red-winged blackbirds (Agelaius phoeniceus), and wild turkeys (Meleagris gallopavo) in 1964. Prior to the 1990s, AC was not registered by the Food and Drug Administration (FDA) for use as an immobilizing agent in the United States for wild animals that might be used for human consumption. In …

Management Strategies For Addressing Wildlife Disease Transmission: The Case For Fertility Control, Gary Killian, Kathleen Fagerstone, Terry Kreeger, Lowell Miller, Jack Rhyan

United States Department of Agriculture Wildlife Services: Staff Publications

Transmissible diseases among wildlife species, and between wildlife and domestic livestock, are a matter of increasing concern. A combination of approaches should be considered for controlling disease transmission. If a vaccine is available or can economically be developed for the disease, it should be used as the primary component of the disease management strategy. For example, development of an oral rabies vaccine has led to programs where baits are dispersed on a large scale in a barrier zone to prevent the spread of rabies. However, there are no vaccines available for many diseases and incentives are frequently not present for …

Avian Influenza In Wild Birds: Environmental Sampling For The Rapid Detection Of Avian Influenza Viruses, Robert G. Mclean, Jeffrey S. Hall, Alan B. Franklin, Heather Sullivan, Kaci K. Vandalen, Susan A. Shriner, Matthew Farnsworth, Paul Oesterle, Ginger Young, Jenny Carlson, Kacy Cobble, Stacey Elmore, Ted Anderson, Sean Hauser, Kevin Bentler, Nicole Mooers, Kathryn P. Huyvaert, Tom Deliberto, Seth Swafford

United States Department of Agriculture Wildlife Services: Staff Publications

All subtypes of influenza Type A viruses infect wild birds, especially waterfowl and shorebirds, but rarely cause disease or mortality in these aquatic species. Aquatic birds are the natural reservoirs for low pathogenic avian influenza viruses (LPAI) that are distributed globally. However, some AI subtypes can be virulent in other animals and humans and some highly pathogenic AI viruses (HPAI) have caused major outbreaks in poultry and even pandemics in the human population. The emergence of a HPAI H5N1 subtype in southeast Asian poultry in 1997 subsequently involved migratory waterfowl in 2005 and has since spread westward throughout the Asian, …

Evidence Of Feline Immunodeficiency Virus, Feline Leukemia Virus, And Toxoplasma Gondii In Feral Cats On Mauna Kea, Hawaii, Raymond M. Danner, Daniel M. Goltz, Steven C. Hess, Paul C. Banko

United States Geological Survey: Staff Publications

We determined prevalence to feline immunodeficiency virus (FIV) antibodies, feline leukemia virus (FeLV) antigen, and Toxoplasma gondii antibodies in feral cats (Felis catus) on Mauna Kea Hawaii from April 2002 to May 2004. Six of 68 (8.8%) and 11 of 68 (16.2%) cats were antibody positive to FIV and antigen positive for FeLV, respectively; 25 of 67 (37.3%) cats were seropositive to T. gondii. Antibodies to FeLV and T. gondii occurred in all age and sex classes, but FIV occurred only in adult males. Evidence of current or previous infections with two of these infectious agents was …

Research Note: Hymenolepis Horrida (Cestoda: Hymenolepididae) And Catenotaenia Peromysci (Cestoda: Anoplocephalidae) In Voles From The Canadian Rockies, Yeen Ten Hwang, Scott Lyell Gardner, John S. Millar

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Cestodes from Clethrionomys gapperi (n=34) and Microtus longicaudus (n=8) from Kootenay National Park, British Columbia, Canada are reported. Two species of cestodes, Hymenolepis horrida (sensu lato) and Catenotaenia peromysci, were found in Clethrionomys gapperi, and one species of cestode, H. horrida, was found in Microtus longicaudus. Clethrionomys gapperi represents a new host record for C. peromysci. The Canadian Rockies is a new locality record for both cestode species.

Multiplication Of Ovaries In Ctenotaenia Marmotae (Frölich, 1802) (Cestoda: Anoplocephalidae), Sumiya Ganzorig, Yuzaburo Oku, Scott Lyell Gardner, Masao Kamiya

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Individuals of the cestode species Ctenotaenia marmotae (Frölich, 1802) (Cestoda: Anoplocephalidae) possessing duplicated rudimentary ovaries toward the medial parts of the segments were found in material collected from the Siberian marmot (Marmota sibirica) in Mongolia. This tapeworm is characterized by possessing one pair of female genitals per segment. The extra rudimentary ovaries that we found ranged from one to six in number per segment and were much smaller than the main pairs of ovaries. Although multiplication of ovaries was reported to occur in a species of Diandrya (Darrah, 1930) by Rausch (1980), this is the first report of …