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2006

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The Guardian, February 15, 2006, Wright State University Student Body Feb 2006

The Guardian, February 15, 2006, Wright State University Student Body

The Guardian Student Newspaper

Twenty-four page issue of The Guardian, the official student-run newspaper for Wright State University. The Guardian has been published regularly since March of 1965.


Occurrence, Persistence, And Expansion Of Saltcedar (Tamarix Spp.) Populations In The Great Plains Of Montana, Jason P. Sexton, Anna Sala, Kevin Murray Feb 2006

Occurrence, Persistence, And Expansion Of Saltcedar (Tamarix Spp.) Populations In The Great Plains Of Montana, Jason P. Sexton, Anna Sala, Kevin Murray

Western North American Naturalist

Saltcedar (Tamarix spp.), a shrub native to Eurasia, is associated with major alterations to wetland and riparian systems in the southwestern United States. Since the 1960s saltcedar has been naturalized in northern states of the U.S. where its growth potential and impacts are not well known. Here, we describe the occurrence, age, size, and relative cover of saltcedar populations in several river basins in central eastern Montana, USA, to identify potential patterns of spread across the region and changes in individual populations as they age. Stands were aged according to the oldest saltcedar individuals and were sampled for dominant …


Composition And Distribution Of The Milliped Tribe Pachydesmini West Of The Mississippi River (Polydesmida: Xystodesmidae), Rowland M. Shelley, Chris T. Mcallister Feb 2006

Composition And Distribution Of The Milliped Tribe Pachydesmini West Of The Mississippi River (Polydesmida: Xystodesmidae), Rowland M. Shelley, Chris T. Mcallister

Western North American Naturalist

The xystodesmid milliped tribe Pachydesmini is supported by the autapomorphic absence of bold aposematic pigmentations and possibly also by relative somatic inflexibility; it comprises 3 component genera: PachydesmusCook, 1895; Dicellarius Chamberlin, 1920; and Thrinaxoria Chamberlin and Hoffman, 1950. Three representatives occur west of the Mississippi River—P. clarus (Chamberlin), P. crassicutis crassicutis (Wood), and T. lampra (Chamberlin). New localities are documented in Louisiana and Texas, and literature records are summarized for these states and Arkansas; records of P. clarus from Cherokee County, Texas, are the westernmost in this state for the east-Nearctic xystodesmid fauna. An apparently allopatric population of …


Community Response Of Nontarget Species To Herbicide Application And Removal Of The Nonindigenous Invader Potentilla Recta L., R. L. Sheley, M. Kirk Denny Feb 2006

Community Response Of Nontarget Species To Herbicide Application And Removal Of The Nonindigenous Invader Potentilla Recta L., R. L. Sheley, M. Kirk Denny

Western North American Naturalist

Our main objective was to improve understanding of herbicide effects on community dynamics to refine the use of technology and advance the development of ecologically based weed management strategies. We hypothesized that native grasslands would exhibit reductions in culturally sensitive forb cover, biomass, and density relative to the rate of application of selective rangeland herbicides, and that hand-removal of sulfur cinquefoil (Potentilla recta L.) would increase indigenous species cover, biomass, density, species richness, and diversity. Treatments consisted of 3 rates each of 2,4-D + clopyralid (0.28 kg ai · ha−1 + 0.0532 kg ai · ha−1, …


Dietary Overlap In Giant Salamanders (Dicamptodon): Applying Null Models To Resource Partitioning, Craig A. Steele, Colin Brammer Feb 2006

Dietary Overlap In Giant Salamanders (Dicamptodon): Applying Null Models To Resource Partitioning, Craig A. Steele, Colin Brammer

Western North American Naturalist

We examined stomach contents of preserved specimens of larval Pacific giant salamander (Dicamptodon tenebrosus) and Cope's giant salamander (D. copei) collected from sympatric and allopatric stream populations. The dietary components of these specimens were used to calculate dietary overlap between the 2 species and to determine if changes in overlap existed between sympatric and allopatric populations. To statistically test overlap values, a randomization algorithm was used to construct a simulated data matrix (i.e., null model) in order to compare observed values of dietary overlap to a distribution of overlap values from the null model. Significant levels …


Introducing The Dragonflies Of British Columbia And The Yukon By Robert A. Cannings, C. Riley Nelson Feb 2006

Introducing The Dragonflies Of British Columbia And The Yukon By Robert A. Cannings, C. Riley Nelson

Western North American Naturalist

No abstract provided.


Recovery And Germinability Of Native Seed Fed To Cattle, Marina K. Whitacre, Christopher A. Call Feb 2006

Recovery And Germinability Of Native Seed Fed To Cattle, Marina K. Whitacre, Christopher A. Call

Western North American Naturalist

Using livestock as seed dispersal agents may be an effective method for increasing species diversity on degraded and previously seeded rangelands. We quantified seed passage and recovery rates, and post-passage germinability of Wyoming big sagebrush (Artemisia tridentata Nutt. ssp. wyomingensis Beetle & Young), bottlebrush squirreltail (Elymus elymoides [Raf.] Swezey), and gooseberry globemallow (Sphaeralcea grossulariaefolia [H. & A.] Rydb.) by feeding Holstein heifers seeds of each species at 3 levels (15,000; 30,000; and 60,000 seeds) over a period of 3 weeks. One-kg fecal samples were collected 1, 2, 3, and 4 days after seed ingestion. Undamaged seeds were …


The Bg News February 15, 2006, Bowling Green State University Feb 2006

The Bg News February 15, 2006, Bowling Green State University

BG News (Student Newspaper)

The BGSU campus student newspaper. February 15, 2006. Volume 96-Issue 101.


Crop Updates 2006 - Oilseeds, Graham Walton, Fiona Martin, Anne Wilkins, Nathan Hancock, Matthew Nelson, Marieclaire Castello, Linda Thompson, Anouska Cousin, Guijun Yan, Wallace Cowling, Moin Salam, Bill Mcloud, Ravjit Khangura, Jean Galloway, Art Diggle, R. F. Brennan, M. D. A. Bolland, P. M. Damon, Z. Rengel, Terry Rose, Qifu Ma, Dave Eksteen Feb 2006

Crop Updates 2006 - Oilseeds, Graham Walton, Fiona Martin, Anne Wilkins, Nathan Hancock, Matthew Nelson, Marieclaire Castello, Linda Thompson, Anouska Cousin, Guijun Yan, Wallace Cowling, Moin Salam, Bill Mcloud, Ravjit Khangura, Jean Galloway, Art Diggle, R. F. Brennan, M. D. A. Bolland, P. M. Damon, Z. Rengel, Terry Rose, Qifu Ma, Dave Eksteen

Crop Updates

This session covers thirteen papers from different authors:

1. INTRODUCTION, Graham Walton, CONVENOR, Department of Agriculture

2. The performance of new TT canola varieties in National Variety Testing (NVT) WA, Fiona Martin, Research Agronomist, Agritech Crop Research

3. Comparison of TT Canola Varieties in Oilseeds WA Trials – 2005, Collated by G.H. Walton, Department of Agriculture, WA, from a collaboration between Oilseeds WA, Seed Companies, Agronomists and Growers

4. An overview of the potential for a Biofuels Industry in Western Australia, Anne Wilkins and Nathan Hancock, Department of Agriculture

5. Retrieval of fertile progeny from interspecific …


Crop Updates 2006 - Cereals, Roger Jones, Nichole Burges, Mohommad Amjad, Ben Curtis, Wal Anderson, Darren Chitty, Brianna Peake, Harm Van Rees, Cherie Reilly, James Hunt, Dean Holzworth, Zvi Hochman, Allan Peake, Neal Dalgliesh, Stephen Van Rees, Trudy Mccann, Peter Carberry, Raj Malik, Kelly Winfield, Brenda Shackley, Judith Devenish, Darshan Sharma, Steve Penny Jr, Christine Zaicou-Kunesch, Mike Bolland, Ross Brennan, Paul Damon, Zed Rengel, Jeremy Lemon, Perry Poulton, Nick Poole, Brooke Thompson, Stephen Loss, Jeff Russell, Angie Roe, James Eyres, Narelle Simpson, Ron Mctaggart, Lionel Martin, Dave Allen, Hossein Golzar, Manisha Shankar, Robert Loughman, John Majewski, Donna Foster, Jamie Piotrowski, Nicole Harry, Geoff Thomas, Kith Jayasena, Svetlana Micic, Paul Matson, Tony Dore, Thomas (Ben) Biddulph, Daryl Mares, Julie Plummer, Tim Setter, Garren Knell, Steve Curtin, Wade Longmuir, Nigel Metz, Tina Botwright Acuña, Len Wade, Cindy Mills, Peter Stone Feb 2006

Crop Updates 2006 - Cereals, Roger Jones, Nichole Burges, Mohommad Amjad, Ben Curtis, Wal Anderson, Darren Chitty, Brianna Peake, Harm Van Rees, Cherie Reilly, James Hunt, Dean Holzworth, Zvi Hochman, Allan Peake, Neal Dalgliesh, Stephen Van Rees, Trudy Mccann, Peter Carberry, Raj Malik, Kelly Winfield, Brenda Shackley, Judith Devenish, Darshan Sharma, Steve Penny Jr, Christine Zaicou-Kunesch, Mike Bolland, Ross Brennan, Paul Damon, Zed Rengel, Jeremy Lemon, Perry Poulton, Nick Poole, Brooke Thompson, Stephen Loss, Jeff Russell, Angie Roe, James Eyres, Narelle Simpson, Ron Mctaggart, Lionel Martin, Dave Allen, Hossein Golzar, Manisha Shankar, Robert Loughman, John Majewski, Donna Foster, Jamie Piotrowski, Nicole Harry, Geoff Thomas, Kith Jayasena, Svetlana Micic, Paul Matson, Tony Dore, Thomas (Ben) Biddulph, Daryl Mares, Julie Plummer, Tim Setter, Garren Knell, Steve Curtin, Wade Longmuir, Nigel Metz, Tina Botwright Acuña, Len Wade, Cindy Mills, Peter Stone

Crop Updates

This session covers twenty nine papers from different authors:

PLENARY

1. The 2005 wheat streak mosaic virus epidemic in New South Wales and the threat posed to the Western Australian wheat industry, Roger Jones and Nichole Burges, Department of Agriculture

SOUTH COAST AGRONOMY

2. South coast wheat variety trial results and best options for 2006, Mohammad Amjad, Ben Curtis and Wal Anderson, Department of Agriculture

3. Dual purpose winter wheats to improve productivity, Mohammad Amjad and Ben Curtis, Department of Agriculture

4. South coast large-scale premium wheat variety trials, Mohammad Amjad and Ben Curtis, Department of Agriculture …


Regulation Of Cytoplasmic Dynein Atpase By Lis1, Mariano T. Mesngon, Cataldo Tarricone, Sachin Hebbar, Aimee Guillotte, E. William Schmitt, Lorene Lanier, Andrea Musacchio, Stephen J. King, Deanna S. Smith Feb 2006

Regulation Of Cytoplasmic Dynein Atpase By Lis1, Mariano T. Mesngon, Cataldo Tarricone, Sachin Hebbar, Aimee Guillotte, E. William Schmitt, Lorene Lanier, Andrea Musacchio, Stephen J. King, Deanna S. Smith

Faculty Publications

Mutations in Lis1 cause classical lissencephaly, a developmental brain abnormality characterized by defects in neuronal positioning.Over the last decade, a clear link has been forged between Lis1 and the microtubule motor cytoplasmic dynein. Substantial evidenceindicates that Lis1 functions in a highly conserved pathway with dynein to regulate neuronal migration and other motile events. Yeasttwo-hybrid studies predict that Lis1 binds directly to dynein heavy chains (Sasaki et al., 2000; Tai et al., 2002), but the mechanistic significance of this interaction is not well understood. We now report that recombinant Lis1 binds to native brain dynein and significantly increases the microtubule-stimulated enzymatic …


Montana Kaimin, February 15, 2006, Students Of The University Of Montana, Missoula Feb 2006

Montana Kaimin, February 15, 2006, Students Of The University Of Montana, Missoula

Montana Kaimin, 1898-present

Student newspaper of the University of Montana, Missoula.


Spartan Daily, February 15, 2006, San Jose State University, School Of Journalism And Mass Communications Feb 2006

Spartan Daily, February 15, 2006, San Jose State University, School Of Journalism And Mass Communications

Spartan Daily (School of Journalism and Mass Communications)

Volume 126, Issue 12


The Spinnaker Vol. 29 No. 22, University Of North Florida Feb 2006

The Spinnaker Vol. 29 No. 22, University Of North Florida

Spinnaker

Student newspaper for the UNF community.


The Effect Of Spiritual Attitudes On Female Hypoactive Sexual Desire Disorder, Ralph W. Fox Ii Feb 2006

The Effect Of Spiritual Attitudes On Female Hypoactive Sexual Desire Disorder, Ralph W. Fox Ii

Doctoral Dissertations and Projects

This study hypothesized that there is a relationship between religious orientation (conservative, moderate, or liberal) and the occurrence of Hypoactive Sexual Desire Disorder (HSDD) among Christian women. The participants of this study were 71 married Christian women who were patients from a Christian obstetrical! gynecological medical practice in southern Georgia and who volunteered for an anonymous survey. The survey instruments used in this study included the Sexual Self-Assessment Questionnaire, the Female Sexual Function Index, and the Christian Religious Orientation Scale. Demographic information was also gathered on each participant.

Results showed that all of the women had a conservative orientation and …


Behavioral And Physiological Responses Of Eared Grebes (Podiceps Nigricollis) To Variations In Brine Shrimp (Artemia Franciscana) Densities, Joe N. Caudell, Michael R. Conover Feb 2006

Behavioral And Physiological Responses Of Eared Grebes (Podiceps Nigricollis) To Variations In Brine Shrimp (Artemia Franciscana) Densities, Joe N. Caudell, Michael R. Conover

Western North American Naturalist

Eared Grebes (Podiceps nigricollis) use the Great Salt Lake (GSL) primarily as a fall staging area. They feed on brine shrimp (Artemia franciscana) to build up energy reserves to complete their southward migration. Whereas it has been speculated that timing of grebe departure from the GSL is due to declining shrimp density, this hypothesis has not been tested. We monitored grebe weight, behavior, and departure dates to test this hypothesis and to determine the effects of declining shrimp densities on Eared Grebes during 1999, 2000, and 2001 when there were different shrimp densities. Despite differences in …


Long-Term Fire History In Great Basin Sagebrush Reconstructed From Macroscopic Charcoal In Spring Sediments, Newark Valley, Nevada, Scott Mensing, Stephanie Livingston, Pat Barker Feb 2006

Long-Term Fire History In Great Basin Sagebrush Reconstructed From Macroscopic Charcoal In Spring Sediments, Newark Valley, Nevada, Scott Mensing, Stephanie Livingston, Pat Barker

Western North American Naturalist

We use macroscopic charcoal analysis to reconstruct fire history in sagebrush (Artemisia tridentata var. wyomingensis and A. tridentata var. tridentata), in Newark Valley, Nevada. We analyzed charcoal at continuous 1-cm intervals (~7–127 years), and pollen at 2- to 10-cm intervals (~70–263 years) in a core spanning the last 5500 cal yr BP (calendar years before present). A charcoal peak in the historic period was associated with a >1400-ha fire dated to 1986 that burned in the watershed. We reconstructed the prehistoric fire history by inferring fires from similar charcoal peaks that were significantly greater than the background charcoal …


Transverse And Longitudinal Variation In Woody Riparian Vegetation Along A Montane River, Jonathan M. Friedman, Gregor T. Auble, Edmund D. Andrews, Gwen Kittel, Richard F. Madole, Eleanor R. Griffin, Tyler M. Allred Feb 2006

Transverse And Longitudinal Variation In Woody Riparian Vegetation Along A Montane River, Jonathan M. Friedman, Gregor T. Auble, Edmund D. Andrews, Gwen Kittel, Richard F. Madole, Eleanor R. Griffin, Tyler M. Allred

Western North American Naturalist

This study explores how the relationship between flow and riparian vegetation varies along a montane river. We mapped occurrence of woody riparian plant communities along 58 km of the San Miguel River in southwestern Colorado. We determined the recurrence interval of inundation for each plant community by combining step-backwater hydraulic modeling at 4 representative reaches with Log-Pearson analysis of 4 stream gaging stations. Finally, we mapped bottomland surficial geology and used a Geographic Information System to overlay the coverages of geology and vegetation. Plant communities were distinctly arrayed along the hydrologic gradient. The Salix exigua Nuttall (sandbar willow) community occurred …


Evaluating Plant Invasions From Both Habitat And Species Perspectives, Geneva W. Chong, Yuka Otsuki, Thomas J. Stohlgren, Debra Guenther, Paul Evangelista, Cynthia Villa, Alycia Waters Feb 2006

Evaluating Plant Invasions From Both Habitat And Species Perspectives, Geneva W. Chong, Yuka Otsuki, Thomas J. Stohlgren, Debra Guenther, Paul Evangelista, Cynthia Villa, Alycia Waters

Western North American Naturalist

We present an approach to quantitatively assess nonnative plant invasions at landscape scales from both habitat and species perspectives. Our case study included 34 nonnative species found in 142 plots (0.1 ha) in 14 vegetation types within the Grand Staircase–Escalante National Monument, Utah. A plot invasion index, based on nonnative species richness and cover, showed that only 16 of 142 plots were heavily invaded. A species invasive index, based on frequency, cover, and number of vegetation types invaded, showed that only 7 of 34 plant species were highly invasive. Multiple regressions using habitat characteristics (moisture index, elevation, soil P, native …


Aspects Of Sacramento Pikeminnow Biology In Nearshore Habitats Of The Sacramento–San Joaquin Delta, California, Matthew L. Nobriga, Frederick Feyrer, Randall D. Baxter Feb 2006

Aspects Of Sacramento Pikeminnow Biology In Nearshore Habitats Of The Sacramento–San Joaquin Delta, California, Matthew L. Nobriga, Frederick Feyrer, Randall D. Baxter

Western North American Naturalist

We documented distribution, relative abundance, diet composition, and body condition of Sacramento pikeminnow Ptychocheilus grandis during 2001 and 2003 at 5 sites in the Sacramento–San Joaquin Delta, California. Sacramento pikeminnow densities in nearshore habitats were higher in 2003 than 2001. In both years, spatial distribution of beach seine densities was similar. There were no significant differences in density among sampling sites except for the southernmost site where the catch was near zero. Based on rotary screw-trap data from a 6th site, we found relative abundance of Sacramento pikeminnow entering the Delta via an artificial floodplain was positively correlated with flow. …


Transborder Associations Of Terrestrial Isopods (Crustacea, Isopoda, Oniscidea) Of Mexico And The United States, Joan P. Jass, Barbara R. Klausmeier Feb 2006

Transborder Associations Of Terrestrial Isopods (Crustacea, Isopoda, Oniscidea) Of Mexico And The United States, Joan P. Jass, Barbara R. Klausmeier

Western North American Naturalist

No abstract provided.


Band-Tailed Pigeon: Wilderness Bird At Risk By Worth Mathewson, Clayton M. White Feb 2006

Band-Tailed Pigeon: Wilderness Bird At Risk By Worth Mathewson, Clayton M. White

Western North American Naturalist

No abstract provided.


The Bates Student - Volume 135 Number 16 - February 14, 2006, Bates College Feb 2006

The Bates Student - Volume 135 Number 16 - February 14, 2006, Bates College

The Bates Student

No abstract provided.


Love And Passion: Man Of Sorrows, Mother Of Compassion, University Of Dayton. Marian Library Feb 2006

Love And Passion: Man Of Sorrows, Mother Of Compassion, University Of Dayton. Marian Library

Marian Library Exhibit Summaries

Archived website and summary of exhibit in the Marian Library

Artist: Ioana Datcu

Exhibit dates: February 14 - March 17, 2006


The Structure Of The Carboxyltransferase Component Of Acetyl-Coa Carboxylase Reveals A Zinc-Binding Motif Unique To The Bacterial Enzyme, Patrick Bilder, Sandra Lightle, Graeme Bainbridge, Jeffrey Ohren, Barry Finzel, Fang Sun, Susan Holley, Loola Al-Kassim, Cindy Spessard, Michael Melnick, Marcia Newcomer, Grover L. Waldrop Feb 2006

The Structure Of The Carboxyltransferase Component Of Acetyl-Coa Carboxylase Reveals A Zinc-Binding Motif Unique To The Bacterial Enzyme, Patrick Bilder, Sandra Lightle, Graeme Bainbridge, Jeffrey Ohren, Barry Finzel, Fang Sun, Susan Holley, Loola Al-Kassim, Cindy Spessard, Michael Melnick, Marcia Newcomer, Grover L. Waldrop

Faculty Publications

Acetyl-coA carboxylase (ACC) is a central metabolic enzyme that catalyzes the committed step in fatty acid biosynthesis: biotin-dependent conversion of acetyl-coA to malonyl-coA. The bacterial carboxyltransferase (CT) subunit of ACC is a target for the design of novel therapeutics that combat severe, hospital-acquired infections resistant to the established classes of frontline antimicrobials. Here, we present the structures of the bacterial CT subunits from two prevalent nosocomial pathogens, Staphylococcus aureus and Escherichia coli, at a resolution of 2.0 and 3.0 Å, respectively. Both structures reveal a small, independent zinc-binding domain that lacks a complement in the primary sequence or structure of …


Stormwater Irrigation Volume I: Evapotranspiration And Nitrate Reduction After Biofiltration To Reduce Health Risks, Martin Wanielista, Ewoud Hulstein Feb 2006

Stormwater Irrigation Volume I: Evapotranspiration And Nitrate Reduction After Biofiltration To Reduce Health Risks, Martin Wanielista, Ewoud Hulstein

BMP Trains Research and Publications

Irrigation of detained stormwater is an option available for volume and pollution control. Volume control is accomplished when detained waters normally discharged from a detention facility are used instead for irrigation. However, a health risk may result if the nitrate in the irrigation water is not attenuated in the plant and soil matrix. The nitrate level in stormwater ponds is generally much less that the health risk specified as 10 mg/L in Drinking water standards. Nevertheless, the fate of nitrate in the soil plant matrix has not been documented. In addition, there are other potential problems associated with not maintaining …


The New Hampshire, Vol. 95 [96], No. 31 (Feb. 14, 2006), The New Hampshire Feb 2006

The New Hampshire, Vol. 95 [96], No. 31 (Feb. 14, 2006), The New Hampshire

The New Hampshire Print Edition

An independent student produced newspaper from the University of New Hampshire. This volume was printed as 95, but it is the 96th volume in the series. This was corrected in pencil.


Garden Show Grows Interest, University Relations Feb 2006

Garden Show Grows Interest, University Relations

Campus News Archive

As a child Sue Gooch viewed gardening as a chore. Now she is so enthused by it that she writes and compiles her own scripts for "Prairie Yard and Garden," the popular show produced by Media Services at UMM and seen by thousands of TV viewers.


Genic Microsatellite Markers Derived From Est Sequences Of Switchgrass (Panicum Virgatum L.), Christian M. Tobias, Daniel M. Hayden, Paul Twigg, Gautam Sarath Feb 2006

Genic Microsatellite Markers Derived From Est Sequences Of Switchgrass (Panicum Virgatum L.), Christian M. Tobias, Daniel M. Hayden, Paul Twigg, Gautam Sarath

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Switchgrass is a large, North American, perennial grass that is being evaluated as a potential energy crop. There is a need to assess genetic diversity in stored accessions and in remaining native stands to assist breeding and conservation efforts. Marker development will also be necessary for genetic linkage mapping. Toward this end, 32 switchgrass genic di-, tri- and tetranucleotide repeat microsatellites were identified from expressed sequence tags (ESTs). These microsatellites were used to screen individuals from two different named cultivars. The markers displayed a high level of polymorphism consistent with the tetraploid, allogamous behaviour of the cultivars tested.


Structural And Function Analyses Of The Global Regulatory Protein Sara From Staphylococcus Aureus, Yingfang Liu, Adhar C. Manna, Cheol-Ho Pan, Irina A. Kriksunov Feb 2006

Structural And Function Analyses Of The Global Regulatory Protein Sara From Staphylococcus Aureus, Yingfang Liu, Adhar C. Manna, Cheol-Ho Pan, Irina A. Kriksunov

Dartmouth Scholarship

The sarA locus in Staphylococcus aureus controls the expression of many virulence genes. The sarA regulatory molecule, SarA, is a 14.7-kDa protein (124 residues) that binds to the promoter region of target genes. Here we report the 2.6 Å-resolution x-ray crystal structure of the dimeric winged helix SarA protein, which differs from the published SarA structure dramatically. In the crystal packing, multiple dimers of SarA form a scaffold, possibly via divalent cations. Mutations of individual residues within the DNA-binding helix–turn–helix and the winged region as well as within the metal-binding pocket implicate basic residues R84 and R90 within the winged …