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Full-Text Articles in Entire DC Network
Folk Wiki: The Shared Traditions Of Folk Music And The Wiki Way, Phillip Mark Chamberlin
Folk Wiki: The Shared Traditions Of Folk Music And The Wiki Way, Phillip Mark Chamberlin
USF Tampa Graduate Theses and Dissertations
Wiki is often perceived as representing a revolutionary break from conventional notions of authorship, writing, and textual history. Dialogues concerning Wiki tend to ignore the characteristics that Wiki shares with earlier forms of collaboration, particularly folk music. In both Wiki and folk music, content is often collectively shared and authored, even if specific individuals create and change the content. Many collaborators are anonymous, quasi-anonymous, or pseudo-anonymous, but the perception of this anonymity is, in both genres, problematic. Second, both Wiki documents and folk songs exist in the "Eternal Now," a seemingly perpetual state that makes these texts available for addition, …
The Influence Of Performance Background On Instrumentalists’ Ability To Discriminate And Label Cornet And Trumpet Timbre, Gary Compton
The Influence Of Performance Background On Instrumentalists’ Ability To Discriminate And Label Cornet And Trumpet Timbre, Gary Compton
USF Tampa Graduate Theses and Dissertations
This study was an examination of timbre discrimination and labeling, with specific consideration of the soprano-brass timbres of cornets and trumpets. This study sought to determine whether instrumentalists differed in their ability to label and discriminate cornet and trumpet timbres, and whether these abilities were influenced by the instrumentalists' performing instrument and years of playing experience. Wind, brass and percussion players (n=100) from existing instrumental groups in the Tampa Bay area participated in the study. These groups were collegiate trumpet majors from a large public university school of music, a collegiate wind ensemble from a private university, and a brass …
Drug Resistant Patterns Of Invasive Streptococcus Pneumoniae Infections In The State Of Florida In 2003, Michael T. Drennon
Drug Resistant Patterns Of Invasive Streptococcus Pneumoniae Infections In The State Of Florida In 2003, Michael T. Drennon
USF Tampa Graduate Theses and Dissertations
Streptococcus pneumoniae is a major bacterial pathogen which causes pneumoniae, meningitis, otitis media, and bacteremia. Currently there are two vaccines available, Pneumococcal Polysaccharide Vaccine (PPV) for adults and the Pneumococcal Conjugate Vaccine (PCV) for children. The PCV vaccine was developed in 2000 specifically for children and infants due to the ineffectiveness of the PPV vaccine in children. This is a cross sectional study of invasive S. pneumoniae in Florida during 2003. This study is designed to determine the population characteristics, clinically relevant antibiotic resistance patterns and specific risk factors for development of antibiotic resistance of invasive S. pneumoniae. Participants for …
Optical Imaging Of Radiolabeled Drugs In Tissue Sections Using The Microimager, Paul Dungel
Optical Imaging Of Radiolabeled Drugs In Tissue Sections Using The Microimager, Paul Dungel
USF Tampa Graduate Theses and Dissertations
The MicroImager is a fast, high resolution, real time, digital autoradiographic imaging tool with broad applications. This study utilizes the MicroImager to evaluate radiolabeled drug behavior in subcutaneous tissue. Experiments were conducted in conjunction with mathematical models to determine the diffusion coefficient (D) and elimination constant (k) for radiolabeled dexamethasone. Osmotic pumps containing [3H]dexamethasone were implanted into rat subcutaneous tissue over 6h, 24 h, and 60 h. Local tissue was explanted and slides were prepared for imaging. The MicroImager was then used to quantify the local concentration of 3H-dexamethasone in the tissue surrounding the tip of the osmotic pump. Betavision+ …
Improving Long-Term Resettlement Services For Refugees, Asylees, And Asylum-Seekers: Perspectives From Service Providers, Kristina M. Dunman
Improving Long-Term Resettlement Services For Refugees, Asylees, And Asylum-Seekers: Perspectives From Service Providers, Kristina M. Dunman
USF Tampa Graduate Theses and Dissertations
In the United States, rights of asylum and refuge are extended to people who can prove that they have been politically persecuted. Resettlement services for refugees and asylees often focus on the short-term acquisition of employment and English language skills. These policies ignore the long-term complexities involved in reestablishing individual and group identities after war trauma and resettlement. This research is an investigation into the perspectives of service providers who work with refugees, asylees, and asylum-seekers on the need for more comprehensive, long-term services to assist in the months and years following resettlement, and on potential programs to address those …
Functional Topology And Regulation Of Endothelial Nitric Oxide Synthase And Associated Caveolar Components, Brenda R. Flam
Functional Topology And Regulation Of Endothelial Nitric Oxide Synthase And Associated Caveolar Components, Brenda R. Flam
USF Tampa Graduate Theses and Dissertations
The discovery of nitric oxide (NO) as the endothelial-derived relaxing factor has led to significant research on NO and the proteins involved in its function, generation, location and regulation. Synthesis of NO by blood vessel endothelial cells results from the enzymatic oxidation of arginine by endothelial nitric oxide synthase (eNOS) resulting in the formation of equimolar amounts of NO and citrulline. Citrulline is sequentially recycled to arginine by successive reactions involving the enzymes argininosuccinate synthase (AS) and argininosuccinate lyase (AL), respectively.
eNOS activity has been shown to be regulated by post-translational modifications including dynamic phosphorylation on multiple serine/threonine and tyrosine …
Alcohol Expectancy Cognitions: Psychophysiological Perspective, Inna Fishman
Alcohol Expectancy Cognitions: Psychophysiological Perspective, Inna Fishman
USF Tampa Graduate Theses and Dissertations
Considerable evidence indicates that the expectations individuals hold about the effects of alcohol determine, to a degree, the amount of alcohol they drink. However, the bulk of this evidence was acquired using verbally-based measures of expectancy. The present study sought to extend the validation network by utilizing an electrophysiological measure -- the P300 component of the Event Related Potentials (ERPs) --
which is thought to index fundamental neurophysiological processes sensitive to expectancy.Previous research has demonstrated that, when presented with various outcomes of alcohol consumption, heavier drinkers endorse statements that assert positive and arousing effects of alcohol, while lighter drinkers endorse …
Calling All Learners: An Explanatory Integrative Research Study Of Efl Learner-Learner Corrective Feedback Patterns Within On-Line Synchronous Environments, Annmarie Gorenc Zoran
Calling All Learners: An Explanatory Integrative Research Study Of Efl Learner-Learner Corrective Feedback Patterns Within On-Line Synchronous Environments, Annmarie Gorenc Zoran
USF Tampa Graduate Theses and Dissertations
This mixed methods research study centers on learner-learner interactions; thus, contributing to the on-going investigation within negotiation and interaction, computer-mediated-communication and its role in second language learning. The specific aim was to investigate corrective feedback types, incidences, and the relationship between error and feedback type among peers within online synchronous environments in EFL classes in Slovenia, Europe. Interactional characteristics of corrective feedback with learners having a documented special need (SN) also were explored using qualitative analyses. The study encompassed 208 students that were randomly placed into 104 dyads within intact classes of Grades 7, 8, 10, and 11. There were …
Modulation Of Neural Plasticity By The Adamtss (A Disintegrin And Metalloproteinase With Thrombospondin Motifs), Michelle Grace Hamel
Modulation Of Neural Plasticity By The Adamtss (A Disintegrin And Metalloproteinase With Thrombospondin Motifs), Michelle Grace Hamel
USF Tampa Graduate Theses and Dissertations
Aggregating proteoglycans (PG) bearing chondroitin sulfate (CS) side chains are well-known inhibitors of neural plasticity and associate with hyaluronan and tenascin-R to form a complex of extracellular matrix (ECM) in the central nervous system (CNS). Little is known about whether proteolytic cleavage of the core protein affects neural plasticity. Several members of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family of metalloproteinases are glutamyl-endopeptidases that cleave aggregating PGs. Our initial studies determined that neural cultures secrete a brevican-containing matrix, and that these neural cultures also produced ADAMTS4, a protease that cleaves brevican. Furthermore, this brevican-containing matrix in astrocytes …
Realism, Sovereignty, And International Relations: An Examination Of Power Politics In The Age Of Globalization, Tyler Harrison
Realism, Sovereignty, And International Relations: An Examination Of Power Politics In The Age Of Globalization, Tyler Harrison
USF Tampa Graduate Theses and Dissertations
Many globalization theorists make the claim that the state, in its current Westphalian context, is no longer a viable unit of analysis in comparative politics or international relations. Globalists claim that in the wake of unprecedented, global integration, the state is either in retreat or on the verge of full scale extinction. In a general sense, this paper explores whether there is a sufficient amount of evidence to supports the claims of globalists that the state is dying. Moreover, the paper looks at the specific issue areas of international trade, multilateralism and the environment to determine what effects globalization has …
The Karst Of West-Central Florida, Lee John Florea
The Karst Of West-Central Florida, Lee John Florea
USF Tampa Graduate Theses and Dissertations
Caves, the cornerstone feature of karst aquifers, are little understood in Florida. This dissertation, which analyzes the morphology, elevation, lithologic setting, and hydrology of caves in west-central Florida, demonstrates that the karst of the unconfined Floridan aquifer differs from the paradigm view of karst presented in modern geology textbooks. The differences reflect setting: eogenetic (west-central Florida) vs. telogenetic (conventional). Interpretations about the architecture of cavernous porosity in this dissertation come from detailed surveys (497 stations) of seven air-filled caves.The surveys reveal that solution cavities within the unconfined Floridan aquifer align along NE-SW and NW-SE fractures. The surveys further identify tabular …
Gold Mining In A Tropical Rainforest: Mercury Sorption To Soils In The Mining Region Of Arakaka-Matthew's Ridge, Guyana, Joniqua A'Ja Howard
Gold Mining In A Tropical Rainforest: Mercury Sorption To Soils In The Mining Region Of Arakaka-Matthew's Ridge, Guyana, Joniqua A'Ja Howard
USF Tampa Graduate Theses and Dissertations
Gold mining by artisinal (small-medium scale) miners causes an immense amount of damage to the environment (i.e. soil erosion, mobilization of heavy metals, etc.).1, 2 One of the most popular gold mining techniques employed by artisinal miners in Guyana is mercury amalgamation. During the amalgamation process approximately 300 metric tons/yr 11, 12 of mercury is used. Mercury once in the environment can be transported through the air, soil, and water column. It is estimated that 90-99% of total mercury (THg) is associated with the sediment. An understanding of the geochemical conditions that affect the fate of mercury in soils, which …
Salinity- And Temperature-Dependent Groundwater Flow In The Floridan Aquifer System Of South Florida, Joseph D. Hughes
Salinity- And Temperature-Dependent Groundwater Flow In The Floridan Aquifer System Of South Florida, Joseph D. Hughes
USF Tampa Graduate Theses and Dissertations
Density-dependent groundwater flow in the Floridan aquifer system (FAS) depends on chloride concentrations and fluid temperature. Previous studies addressing the role of chloride concentration and temperatures on groundwater flow in the FAS have relied on observation data or simplified two-dimensional numerical models. A three-dimensional hydrologic analysis of FAS in peninsular Florida was performed using a modified version of SUTRA (SUTRA-MS) capable of simulating multi-species solute and heat transport. SUTRA-MS was developed during this investigation and is capable of reproducing results for several problems with known solutions.The model was developed using available geometric and hydraulic parameter data and calibrated using hydraulic …
Differential Coupling Of Rgs3s And Rgs4 To Gpcr-Girk Channel Signaling Complexes, Cristina Jaén
Differential Coupling Of Rgs3s And Rgs4 To Gpcr-Girk Channel Signaling Complexes, Cristina Jaén
USF Tampa Graduate Theses and Dissertations
'Regulators of G protein signaling' (RGS proteins) modulate the G proteincycle by enhancing the GTPase activity of Ga subunits. These changesaccelerate the kinetics of ion channel modulation by Gai/o-coupled receptors(GPCRs) such as the G protein-gated inward rectifier K+ (GIRK/Kir3) channel. Myexperiments indicate that a single cerebellar granule (CG) neuron, a cell type thatendogenously expresses GIRK channels is able to express a wide variety ofRGS proteins. I selected two of them, which are widely expressed andtranscriptionally regulated during pathophysiologic conditions, to compare theirfunctional properties. I originally described the differential modulatory effects oftwo RGS proteins, the RGS3 short isoform (RGS3s) and RGS4, …
Tyrosinase-Like Activity Of Several Alzheimer’S Disease Related And Model Peptides And Their Inhibition By Natural Antioxidants, Kashmir Singh Juneja
Tyrosinase-Like Activity Of Several Alzheimer’S Disease Related And Model Peptides And Their Inhibition By Natural Antioxidants, Kashmir Singh Juneja
USF Tampa Graduate Theses and Dissertations
Neurodegenerative diseases are associated with loss of neurons ultimately leading to a decline in brain function. Alzheimer's disease (AD) is considered one of the most common neurodegenerative disorders that affects 16 million people worldwide. The cause of the disease remains unknown, although significant evidence proposes the amyloid Beta-peptide (A-Beta) as a potential culprit. The binding of Cu2+ by the soluble fragments of A-Beta have shown to form Type-3 copper centers and catalyze the oxidation of catechol-containing neurotransmitters. Furthermore, the use of flavonoids as antioxidants to slow or inhibit the neurotransmitter oxidation has suggested further health benefits with their consumption. A …
The Realism Of Hans Morgenthau, Brian A. Keaney
The Realism Of Hans Morgenthau, Brian A. Keaney
USF Tampa Graduate Theses and Dissertations
This thesis analyzes the life and career of Hans J. Morgenthau, commonly accepted as the father of the realist paradigm within the field of international relations. It does so by offering a dynamic revisionist account of the nature of Morgenthau's classical realism and suggests how the approach discussed might have wider application in the field of international relations. Traditional perspectives of Morgenthau suggest that in the course of his career, he changed from holding what would generally be labeled a conservative political viewpoint to a liberal political viewpoint. This thesis takes a different starting point. It does so by suggesting …
Structured Light For Three-Dimensional Microscopy, Leo G. Krzewina
Structured Light For Three-Dimensional Microscopy, Leo G. Krzewina
USF Tampa Graduate Theses and Dissertations
The conventional light microscope is an indispensable tool for many physical and life science applications, but is of limited usefulness for three-dimensional imaging due to its increasingly narrow depth of field at high magnifications. Focused regions may be obscured by defocused neighbors or noise from extraneous light sources and subsurface scattering. By rejecting light originating from outside the depth of focus it is possible to minimize these problems. When a contiguous series of such focused slices, or optical sections, are obtained along an axis of an extended object they may be combined to form a complete, focused three-dimensional surface image. …
Part-Time Faculty Job Satisfaction: A Study Of The Influence Of Instructional Technology On Part-Time Faculty In Post-Secondary Institutions, John P. Kurnik
Part-Time Faculty Job Satisfaction: A Study Of The Influence Of Instructional Technology On Part-Time Faculty In Post-Secondary Institutions, John P. Kurnik
USF Tampa Graduate Theses and Dissertations
In 1990, two-year colleges nationwide reported that approximately 38% of their faculty were part-time. According to the National Center for Educational Statistics' (NCES) 1999 National Study of Post-Secondary Faculty (NSOPF), this percentage continues to rise, and currently exists at 40% or more in some two-year and four-year institutions. To retain competent, qualified, and successful teachers, it is critical for higher education administrators to determine factors that may contribute to part-time faculty's job satisfaction. This study investigated whether the use of instructional technology for curriculum delivery affected part-time faculty job satisfaction by investigating four specific areas that may be affected. The …
Occupancy And Function Of The Hepatic Hmg-Coa Reductase Promoter, William Raymond Lagor
Occupancy And Function Of The Hepatic Hmg-Coa Reductase Promoter, William Raymond Lagor
USF Tampa Graduate Theses and Dissertations
HMG-CoA reductase (HMGR) catalyzes the rate controlling step in cholesterol production. This enzyme is highly expressed in the liver where it is subject to extensive hormonal and dietary regulation. This study was undertaken to examine the occupancy and function of the hepatic HMGR promoter in regards to insulin and sterol regulation. HMGR protein and mRNA are substantially decreased in diabetic animals and rapidly restored by administration of insulin. Nuclear run-on assays revealed that HMGR transcription was substantially reduced in the diabetic rats, and fully restored within two hours after insulin treatment. In vivo footprinting revealed several areas of protein binding …
Electrically Charged Sol-Gel Coatings For On-Line Preconcentration And Analysis Of Zwitterionic Biomolecules By Capillary Electrophoresis, Wen Li
USF Tampa Graduate Theses and Dissertations
Novel on-line methods are presented for the extraction, preconcentration and analysis of zwitterionic biomolecules using sol-gel-coated columns coupled to a conventional UV/visible detector. The presented approaches do not require any additional modification of the commercially available standard CE instrument. Extraction, stacking, and focusing techniques were used in the preconcentration procedures. The positively charged sol-gel coatings were created using N-octadecyldimethyl[3-(trimethoxysilyl) proply]ammonium chloride (C18-TMS) in the coating sol solutions. Due to the presence of a positively charged quaternary ammonium moiety in C18-TMS, the resulting sol-gel coating carried a positive charge. The negatively charged sol-gel coatings were due to the presence of sulfonate …
Quantitative Biological Microscopy By Digital Holography, Christopher J. Mann
Quantitative Biological Microscopy By Digital Holography, Christopher J. Mann
USF Tampa Graduate Theses and Dissertations
In this dissertation, improved techniques in digital holography, that have produced high-resolution, high-fidelity images, are discussed. In particular, the angular spectrum method of calculating holographic optical field is noted to have several advantages over the more commonly used Fresnel transformation or Huygens convolution method. It is observed that spurious noise and interference components can be tightly controlled through the analysis and filtering of the angular spectrum. In the angular spectrum method, the reconstruction distance does not have a lower limit, and the off-axis angle between the object and reference waves can be lower than that of the Fresnel requirement, while …
Endothelial Cell Mediators Of Angiogenesis InBartonella Henselae Infection, Amy M. Mccord
Endothelial Cell Mediators Of Angiogenesis InBartonella Henselae Infection, Amy M. Mccord
USF Tampa Graduate Theses and Dissertations
Bacillary angiomatosis (BA), one of the clinical manifestations resulting from infection with the facultative intracellular bacterium Bartonella henselae, is characterized by angiogenic lesions. Endothelial cells have been identified as host cells for this pathogen and are presumed important for pathogenesis as lesions contain bacteria in direct contact with the endothelium. Lesions also contain infiltrating macrophages, which contribute to the angiogenic process during B. henselae infection by secreting vascular endothelial growth factor (VEGF). While virulence factors have been characterized, and the role for macrophages in B. henselae infection has been established, endothelial cell mediators of angiogenesis have not been well defined. …
Analysis Of Acoustic Emission In Cohesionless Soil, Jeyisanker Mathiyaparanam
Analysis Of Acoustic Emission In Cohesionless Soil, Jeyisanker Mathiyaparanam
USF Tampa Graduate Theses and Dissertations
Acoustic emission is a widely used nondestructive technique for identification of structural damage. The AE technique relies on transient energy waves generated by the materials during their failure. As for soils, the basic causes of acoustic emission are the mechanisms which are responsible for shearing of soils. Mobilization of shear strength within a soil itself and the interaction of the soil with the adjacent natural or construction materials are directly related to the level of acoustic emission in soils. It is envisioned that acoustic emission signals in deforming soils can be used as an early warning sign in real time …
A Design Of Experiments Study Of Procedure For Assembling Bascule Bridge Fulcrum, Cuong Q. Nguyen
A Design Of Experiments Study Of Procedure For Assembling Bascule Bridge Fulcrum, Cuong Q. Nguyen
USF Tampa Graduate Theses and Dissertations
A bascule bridge is a type of movable bridge that can be opened or closed to facilitate the movement of water-borne traffic such as ships and yachts. Trunnion-Hub-Girder (THG) assembly plays a role as a fulcrum in the bascule bridge. To make the fulcrum, the trunnion is shrink-fitted into the hub, and then the trunnion-hub assembly is shrink-fitted into the girder. Hundreds of thousands of dollars could be lost due to failures during this step. Crack formations in the hubs of various Florida bascule bridges during assembly led the Florida Department of Transportation to commission a project with USF professors …
Breast Cancer: Relationship Between Acculturation And Barriers To Breast Cancer Screening In Southwest Florida Latinas, Patricia Patino
Breast Cancer: Relationship Between Acculturation And Barriers To Breast Cancer Screening In Southwest Florida Latinas, Patricia Patino
USF Tampa Graduate Theses and Dissertations
Despite multiple campaigns by the American Cancer Society, reports indicate that Latinas living in the United States who contract breast cancer are more likely than Anglos to die. These findings correlate with low participation in breast cancer screenings among Latinas. The objective of this study was to identify key obstacles that influence Latinas' low participation in breast cancer screenings, based on their health beliefs, knowledge of screenings, acculturation, and socio-economic factors.The study was a face-to-face informal interview, combined with a survey questionnaire conducted at churches, social clubs and/or at the participants' homes in a southwest Florida urban community. The sample …
Archival Evaluation Of A Proactive School-Wide Discipline Plan, Beth Rutz-Beynart
Archival Evaluation Of A Proactive School-Wide Discipline Plan, Beth Rutz-Beynart
USF Tampa Graduate Theses and Dissertations
The study conducted was an archival review of school detailed incident discipline records and description of the school-wide proactive discipline plan developed at an elementary school. The study examined the effects of sequential changes in a proactive school-wide discipline plan. Initially, the baseline data consisted of a full year of school without a proactive school-wide discipline plan. This allowed an assessment of the variation in referrals that occurred across a school year. Subsequent years were assessed in relation to this baseline, and the effects from year to year compared to other years. After the baseline year, substantial changes were made …
The Social Construction Of School Refusal: An Exploratory Study Of School Personnel’S Perceptions, Anna Marie Torrens Salemi
The Social Construction Of School Refusal: An Exploratory Study Of School Personnel’S Perceptions, Anna Marie Torrens Salemi
USF Tampa Graduate Theses and Dissertations
Despite a multi-disciplinary, international literature, little research has drawn attention to the phenomenon of school refusal within the school. Most research on school refusal follows a positivist paradigm, focusing on the student, instead of examining the role of schools. Using a qualitative design and a social constructionist framework, this study explored how school personnel perceive school refusal, focusing on the social interactions, processes, and perceptions that construct their understanding. The study was conducted in a large school district in the Southeastern United States.Semi-structured in-depth interviews were conducted with school personnel at the middle school (N=42), high school (N=40), and district …
Assessment Of Biogeochemical Deposits In Landfill Leachate Drainage Systems Phase Ii, Abdul R. Mulla Saleh
Assessment Of Biogeochemical Deposits In Landfill Leachate Drainage Systems Phase Ii, Abdul R. Mulla Saleh
USF Tampa Graduate Theses and Dissertations
Land disposal of solid waste is a vital component of any solid waste management system. Design, operation and closure of municipal solid waste (MSW) landfills are required by regulations to control leachate and gases generated during the life, closure,and post-closure of the facility. Clogging of leachate drainage and removal systems in landfills is a common phenomenon and has been acknowledged in several landfills throughout the United States and abroad. This project was conducted in two phases. Phase I was completed in February of 2005 and Phase II was completed in August of 2006. Leachate characteristics data obtained in Phase I …
Correlates Of Weight In Adolescents: A Path Analysis, Angela T. Sheble
Correlates Of Weight In Adolescents: A Path Analysis, Angela T. Sheble
USF Tampa Graduate Theses and Dissertations
This study examined the interrelationships between adolescent weight and several other variables thought to impact weight and obesity: physical activity, depressive symptoms, binge-eating symptoms, dieting, socio-economic status, special education status, gender, and ethnicity. The sample consisted of 251 high school students in rural Florida who completed measures of depression, binge-eating, dieting, and physical activity. Measurement instruments included the Reynolds Adolescent Depression Scale-2nd Edition (RADS-2), the bulimia scale of the Eating Disorder Inventory-2 (EDI-2), the dieting scale of the Dutch Eating Behavior Questionnaire (DEBQ), and a physical activity questionnaire derived from the Youth Risk Behavior Scale for Students (YRBSS). The study …
Heme Biosynthesis: Structure–Activity Studies Of Murine Ferrochelatase, Zhen Shi
Heme Biosynthesis: Structure–Activity Studies Of Murine Ferrochelatase, Zhen Shi
USF Tampa Graduate Theses and Dissertations
Ferrochelatase catalyzes the terminal step of heme biosynthesis by inserting ferrous iron into protoporphyrin IX. The current study is aimed at understanding the structural basis of porphyrin binding and distortion in ferrochelatase-catalyzed reaction by functional analysis of a highly conserved active site loop motif. The loop was shown to contact bound porphyrin based on crystallographic and molecular modelling observations, and its role in murine ferrochelatase was assessed by random mutagenesis and steady-state kinetic analysis. To overcome the limitations of conventional kinetic assay methods for ferrochelatase, a continuous assay was developed by monitoring porphyrin fluorescence decrease using natural substrates ferrous iron …