Digital Commons Network^™

Acquisition And Retention Of Bacterial Spores (Bacillus Atrophaeus) By Eight Insect Species, Kieron Marie Torres

Theses and Dissertations

Acquisition and retention of spores of an anthrax surrogate, Bacillus atrophaeus Nakamura ("BG") were evaluated in eight insect species. Species included: house cricket (Acheta domesticus L.), German cockroach (Blatella germanica L.), common house fly (Musca domestics L.), blue bottle fly (Calliphora vomitoria L.), hairy rove beetle Creophilus maxillosus L.), yellow mealworm (Tenebrio molitor L.), common paper wasps (Polistes exclamans exclamans Viereck), red paper wasps (Polistes Carolina L.), red harvester ant (Pogonymyrmex barbatus Smith). Individual insects were offered BG-treated food and sacrificed at specified time intervals following one, two or three meals. Resulting samples were surface-washed five consecutive times then homogenized …

The Antiviral Action Of Common Household Disinfectants And Antiseptics Against Murine Hepatitis Virus, Christine Bernadette Dellanno

Theses, Dissertations and Culminating Projects

The 2003 outbreak of Severe Acute Respiratory Syndrome (SARS) infected over 8,000 people and killed 784 leaving many questions concerning the effectiveness of common household disinfectants and antiseptics at preventing viral transmission. In order to determine how well standard disinfectants and detergents were at eliminating viral particles from surfaces, the antiviral action of triclosan, pine oil, bleach, chloroxylenol and quaternary ammonium compound/ethanol based products were assayed against Murine Hepatitis Virus (MHV), a virus genetically similar to SARS. Using the Environmental Protection Agency (EPA) guidelines for the virucidal assay, it was determined that alkyl dimethyl benzyl ammonium saccharinate in 79% ethanol …

Use Of Polyhydroxyalkanoates For Denitrification In Recirculating Aquaculture Systems, Maria Teresa Gutierrez-Wing

LSU Doctoral Dissertations

Increasing demand of aquacultural products is driving the industry toward more intensive culture practices. Aquacultural operations are constrained by limitations on the availability high quality waters and regulatory pressures on the discharges. Recirculating systems can address these issues by reducing the amount of water required in the operation. Closed systems are especially suited where biosecurity is important. In closed systems, nitrate accumulates, limiting the time the water can be reused. In marine inland systems, water reuse reduces the cost of salt for make up water. To increase the water reuse time, some form of nitrate removal process must be incorporated …

Physical And Health Assessment Of A Population Of Raccoon (Procyon Lotor) In Northeastern Florida, Eric C. Munscher

UNF Graduate Theses and Dissertations

The purpose of this research was to study the potential negative effects that mesopredators have on their environment and to promote control of mesopredator populations. Overabundant predatory species such as the raccoons (Procyon lotor) and Virginia opossums (Didelphis virginiana) can have significant pernicious effects on populations of autochthonous prey species, particularly when super predators such as the red wolf (Canis rufus) and Florida panther (Felis concolor coryii) are absent. These overabundant species, coined as mesopredators, are often responsible for extreme levels of predation on prey species and/or their young. The mesopredator release hypothesis involves the "release" or increased density of …

Sharp Increases In Heating Prices And Limited Government Assistance Spark Concerns About Potential Home Heating Crisis, Claire Mariano

Public Interest Law Reporter

No abstract provided.

Outlook Magazine, Fall 2006

Outlook Magazine

No abstract provided.

Washington University Magazine, Spring 2006

Washington University Magazine

No abstract provided.

Outlook Magazine, Winter 2006

Outlook Magazine

No abstract provided.

Literacy And Recent History Of Diarrhoea Are Predictive Of Plasmodium Falciparum Parasitaemia In Kenyan Adults, Rodney Coldren, Trish Prosser, Fredrick Ogolla, Victor Ofula

Research outputs pre 2011

Background - Malaria is one of the most serious health problems in Kenya. In 2004, the Kenya Medical Research Institute and the US Army Medical Research Unit – Kenya surveyed adults in Samburu, Malindi, and Busia districts to determine socioeconomic risk factors for infection.

Methods - Sociodemographic, health, and antimalarial data were collected along with blood for malaria testing. A smear was considered negative only if no Plasmodium falciparum parasites were observed in 100 high-powered fields. Univariate analysis was performed with Pearson's Chi-square test and univariate logistic regression. A multivariate logistic regression model was then created which included only variables …

Free To Be Obese In A 'Super Nanny State'?, Nadine Henley

Research outputs pre 2011

Should individuals be free to make lifestyle decisions (such as what, when and how much to eat and how much physical activity to take), without undue interference from the state, even when their decisions may lead to negative consequences (obesity, heart disease, diabetes)?

Enhanced Disease Surveillance Through Private Health Care Sector Cooperation In Karachi, Pakistan: Experience From A Vaccine Trial, M. Imran Khan, Shah Muhammad Sahito, Mohammad Javed Khan, Shafi Mohammad Wassan, Abdul Wahab Shaikh, Ashok Kumari Maheshwari, Camilo J. Acosta, Claudia M. Galindo, Rion Leon Ochia, Shahid Rasool, Sheeraz Peerwan, Mahesh K. Puri, Mohammad Ali, Afia Zafar, Rumina Hasan, Lorenz Von Seidlein, John D. Clemens, Shaikh Qamaruddin Nizami, Zulfiqar Ahmed Bhutta

Centre of Excellence in Women and Child Health

INTRODUCTION: In research projects such as vaccine trials, accurate and complete surveillance of all outcomes of interest is critical. In less developed countries where the private sector is the major health-care provider, the private sector must be included in surveillance systems in order to capture all disease of interest. This, however, poses enormous challenges in practice. The process and outcome of recruiting private practice clinics for surveillance in a vaccine trial are described.
METHODS: The project started in January 2002 in two urban squatter settlements of Karachi, Pakistan. At the suggestion of private practitioners, a phlebotomy team was …

Rehabilitation After Brain Injury : Identifying Predictors Throughout Post Acute Rehabilitation, Elisabeth L. Bond

PCOM Psychology Dissertations

The purpose of this study was to investigate the functional outcomes of clients who received post-acute rehabilitation services following a Traumatic Brain Injury (TBI) in an effort to identify predictors affecting outcome. An archival database was used to study three different outcome measure ratings over two and or three consecutive time periods. There were 105 participants, 82 male, 23 female, 87 residential and 18 outpatient, who were 19-78 years of age. A multivariate analysis of variance (MANOVA) was conducted within subjects to identify changes across time. No significant changes were found. A between subjects MANOVA was conducted to study relationships …

Toward Facilitating A Voice For Politically Marginalized Minorities And Enhancing Presidential Public Accountability And Transparency In Foreign Health Policymaking, Nina J. Crimm

Vanderbilt Journal of Transnational Law

Residents of underdeveloped countries who belong to ethnic, racial, sexual, and political minorities usually endure relatively ineffective political voices. More than any other world population segment, these marginalized people are vulnerable to, and suffer from, compromised health and life expectancies. Their immense human tolls have spawned severe global humanitarian, economic, social, political, and security dilemmas contrary to the strategic interests of the United States. Despite recognition of these devastating harms here and abroad, the president as de facto primary U.S. foreign policymaker continues to formulate foreign health policy in an insular policymaking environment. The insularity enables the president to design …

Feminizing Waste: Waste-Picking As An Empowerment Opportunity For Women And Children In Impoverished Communities, Catherine A. Madsen

Colorado Environmental Law Journal

No abstract provided.

An Investigation Of The Chemistry And Antimalarial Activity Of The Marine Soft Coral Asterospicularia Randalli, S. Duane Burt

Electronic Theses and Dissertations

No abstract provided.

Innate Antiviral Response Targets Hiv-1 Release By The Induction Of Ubiquitin-Like Protein Isg15, Atsushi Okumura, Gengshi Lu, Ian Pitha-Rowe, Paula M. Pitha

Dartmouth Scholarship

The goal of this study was to elucidate the molecular mechanism by which type I IFN inhibits assembly and release of HIV-1 virions. Our study revealed that the IFN-induced ubiquitin-like protein ISG15 mimics the IFN effect and inhibits release of HIV-1 virions without having any effect on the synthesis of HIV-1 proteins in the cells. ISG15 expression specifically inhibited ubiquitination of Gag and Tsg101 and disrupted the interaction of the Gag L domain with Tsg101, but conjugation of ISG15 to Gag or Tsg101 was not detected. The inhibition of Gag-Tsg101 interaction was also detected in HIV-1 infected, IFN-treated cells. Elimination …

Epidemiological Models For Mutating Pathogens With Temporary Immunity, Neeta Singh

Electronic Theses and Dissertations

Significant progress has been made in understanding different scenarios for disease transmissions and behavior of epidemics in recent years. A considerable amount of work has been done in modeling the dynamics of diseases by systems of ordinary differential equations. But there are very few mathematical models that deal with the genetic mutations of a pathogen. In-fact, not much has been done to model the dynamics of mutations of pathogen explaining its effort to escape the host's immune defense system after it has infected the host. In this dissertation we develop an SIR model with variable infection age for the transmission …

Experimental Infection Of Fox Squirrels (Sciurus Niger) With West Nile Virus, J. Jeffrey Root, Paul T. Oesterle, Nicole M. Nemeth, Kaci Klenk, Daniel H. Gould, Robert G. Mclean, Larry Clark, Jeffrey S. Hall

United States Department of Agriculture Wildlife Services: Staff Publications

Tree squirrels (Sciurus spp.) have exhibited high seroprevalence rates, suggesting that they are commonly exposed to West Nile virus (WNV). Many characteristics of WNV infections in tree squirrels, such as the durations and levels of viremia, remain unknown. To better understand WNV infections in fox squirrels (S. niger), we subcutaneously inoculated fourteen fox squirrels with WNV. Peak viremias ranged from 10^4.00 plaque-forming units (PFU)/mL of serum on day 2 post-infection (DPI) to 10^4.98 PFU/mL on 3 DPI, although viremias varied between individuals. Oral secretions of some fox squirrels were positive for WNV viral RNA, occasionally …

Distribution Of The Deer Tick (Ixodes Scapularis) In A Corridor Between Marquette And Menominee In The Upper Peninsula Of Michigan, Kimberly Jaclynn Miedema

All NMU Master's Theses

No abstract provided.

Mechanisms Of The Persistence Of Antibiotic Resistant Enteric Bacteria In Freshwater Wetland Environments, Andrekeus Lee

Electronic Theses and Dissertations

No abstract provided.

Progress In Diagnosis, Treatment And Elimination Of Echinococcosis And Cysticercosis, Peter M. Schantz

Zoonotics and Wildlife Disease: Publications

Here I review the current status of geographical occurrence and public health significance of echinococcosis (Echinococcus spp. infections) and cysticercosis (Taenia solium infection) with special emphasis on the remarkable technologic progress achieved in recent decades that has led to greater understanding of the biology and epidemiology of these cestode infections. The greatest remaining challenges are to apply this knowledge and technology to improved medical management and prevention of these infections.

Intercellular Spreading Of Porphyromonas Gingivalis Infection In Primary Gingival Epithelial Cells, Özlem Yilmaz, Philippe Verbeke, Richard J. Lamont, David M. Ojcius

All Dugoni School of Dentistry Faculty Articles

Porphyromonas gingivalis, an important periodontal pathogen, is an effective colonizer of oral tissues. The organism successfully invades, multiplies in, and survives for extended periods in primary gingival epithelial cells (GECs). It is unknown whether P. gingivalis resides in the cytoplasm of infected cells throughout the infection or can spread to adjacent cells over time. We developed a technique based on flow cytofluorometry and fluorescence microscopy to study propagation of the organism at different stages of infection of GECs. Results showed that P. gingivalis spreads cell to cell and that the amount of spreading increases gradually over time. There was a …

In Vitro Toxicity Assessment Of Silver Nanoparticles In Rat Alveolar Macrophages, Cataleya Carlson

Browse all Theses and Dissertations

The present study was conducted to assess the toxicity of nanosized silver particles (Ag-15nm, 30nm and 55nm) in rat alveolar macrophages. For toxicity evaluations, cellular morphology, mitochondrial function (MTT assay), membrane leakage (LDH assay) and reactive oxygen species generation (ROS) were assessed after a 24h under control and exposed conditions. The morphological appearance of control and exposed cells were observed by an inverted, phase contrast microscope and the uptake of nanoparticles was observed using the CytoViva Ultra Resolution Imaging (URI) system. Morphological images captured at 100x magnification demonstrated that nanoparticle-exposed cells at higher doses became abnormal in size, and most …

Susceptibility Of Primary Eosinophils To Infection With Hiv-1 Strain Htlv-Iiib, Jai Govind Marathe

Browse all Theses and Dissertations

Over the past two decades, much research has been done in the field of human immunodeficiency virus (HIV) and acquired immunodeficiency syndrome (AIDS). However, many of the aspects of pathogenesis of HIV infection and its persistence in the body, despite treatment, remain a mystery. Recent evidence suggests that HIV positive patients develop eosinophilia, especially in the later stages of infection and AIDS. Eosinophils are CD4 positive cells that have the potential to be infected by HIV. Studies have shown that an eosinophilic cell line, AML14.3D10, can be productively infected with a T-cell tropic, CXCR4-using (X4) strain of HIV-1. In this …

Phenotypic Characterization Of Escherichia Coli Strains Taken From Human Intestinal And Urinary Tracts, Kruthi Murthy

Browse all Theses and Dissertations

Escherichia coli (E. coli) is a gram negative bacterium commonly found as a commensal in the intestinal tract of humans and other warm blooded animals. The commensal strains of E. coli are non-pathogenic and do not cause an infection in the host. However, some strains of E. coli are pathogenic and can cause several diseases in humans that include neonatal meningitis, intestinal infections and urinary tract infections. Almost 80-85% of the uncomplicated urinary tract infections are caused by uropathogenic E. coli. Our project involved the characterization of 12 strains of E. coli, isolated from humans in health and disease. These …

Colloquy: Moving Forward In Food Safety & Defense, Julie Ostrowsky

Minnesota Journal of Law, Science & Technology

No abstract provided.

Myxozoan Genera: Definition And Notes On Taxonomy, Life-Cycle Terminology And Pathogenic Species, Jiří Lom, Iva Dyková

Harold W. Manter Laboratory of Parasitology: Library Materials

A list of myxozoan genera is presented in the current taxonomical scheme. These genera are defined; their type species and most important pathogens along with their hosts are listed. Simultaneously, definitions of actinospore stages representing sexual stages of the myxosporean life cycle are given; altogether, 17 actinospore collective groups with 180 types have been described. Life cycles of the two classes of the phylum Myxozoa, Malacosporea and Myxosporea, are briefly outlined with specification of the appropriate terms. Up to now, 4 malacosporean and 2,180 myxosporean species assigned to a total of 62 genera, have been established. The surviving classification of …

Long-Term Reduction Of Trypanosoma Cruzi Infection In Sylvatic Mammals Following Deforestation And Sustained Vector Surveillance In Northwestern Argentina, L. A. Ceballos, M. V. Cardinal, G. M. Vazquez-Prokopec, M. A. Lauricella, M. M. Orozco, M. Roberto Cortinas, A. G. Schijman, M. J. Levin, Uriel Kitron, R. E. Gürtlera

Department of Entomology: Faculty Publications

Long-term variations in the dynamics and intensity of sylvatic transmission of Trypanosoma cruzi were investigated around eight rural villages in the semiarid Argentine Chaco in 2002–2004 and compared to data collected locally in 1984–1991. Of 501 wild mammals from 13 identified species examined by xenodiagnosis, only 3 (7.9%) of 38 Didelphis albiventris opossums and 1 (1.1%) of 91 Conepatus chinga skunks were infected by T. cruzi. The period prevalence in opossums was fourfold lower in 2002–2004 than in 1984–1991 (32–36%). The infection prevalence of skunks also decreased five-fold from 4.1–5.6% in 1984–1991 to 1.1% in 2002–2004. Infection in opossums increased …

Design Clues From Functional Constraints And Broadly Neutralizing Antibodies, Tongqing Zhou, Ling Xu, Barna Dey, Ann J. Hessell, Shahzad Majeed, Donald Van Ryk, Shi-Hua Xiang, Xinzhen Yang, Mei-Yun Zhang, Michael B. Zwick, James Arthos, Dennis R. Burton, Dimiter S. Dimitrov, Joseph Sodroski, Richard Wyatt, Gary J. Nabel, Peter D. Kwong

Nebraska Center for Virology: Faculty Publications

No abstract provided.

Cross-Subtype T-Cell Immune Responses Induced By A Human Immunodeficiency Virus Type 1 Group M Consensus Env Immunogen, Eric A. Weaver, Zenaido T. Camacho, Fatiha Moukdar, Hua-Xin Liao, Ben-Jiang Ma, Mark Muldoon, James Theiler, Gary J. Nabel, Norman L. Letvin, Bette T. Korber, Beatrice H. Hahn, Barton F. Haynes, Feng Gao, Zhongjing Lu

Nebraska Center for Virology: Faculty Publications

The genetic diversity among globally circulating human immunodeficiency virus type 1 (HIV-1) strains is a serious challenge for HIV-1 vaccine design. We have generated a synthetic group M consensus env gene (CON6) for induction of cross-subtype immune responses and report here a comparative study of T-cell responses to this and natural strain env immunogens in a murine model. Three different strains of mice were immunized with CON6 as well as subtype A, B, or C env immunogens, using a DNA prime-recombinant vaccinia virus boost strategy. T-cell epitopes were mapped by gamma interferon enzyme-linked immunospot analysis using five overlapping Env peptide …