Digital Commons Network^™

Genetically Engineered Human Mesenchymal Stem Cells Produce Met-Enkephalin At Augmented Higher Levels In Vitro, Ikuko Sugaya, Tingyu Qu, Kiminobu Sugaya, George D. Pappas

Faculty Bibliography 2000s

We have reported that transplantation of adrenal medullary chromaffin cells that release endogenous opioid peptides into pain modulatory regions in the CNS produce significant antinociceptive effects in patients with terminal cancer pain. However, the usefulness of this procedure is minimal because the availability of human adrenal tissue is very limited. Alternative xenogeneic materials, such as porcine and bovine adrenal chromaffin cells present problems of immune rejection and possible pathogenic contamination. In an attempt to develop opioid peptide-producing cells of autologous origin, we have transfected human mesenchymal stem cells (hMeSCs) with a mammalian expression vector containing a fusion gene of green …

Whole-Genome Plasticity Among Mycobacterium Avium Subspecies: Insights From Comparative Genomic Hybridizations, Chia-Wei Wu, Jeremy Glasner, Michael Collins, Saleh Naser, Adel M. Talaat

Faculty Bibliography 2000s

Infection with Mycobacterium avium subsp. paratuberculosis causes Johne's disease in cattle and is also implicated in cases of Crohn's disease in humans. Another closely related strain, M. avium subsp. avium, is a health problem for immunocompromised patients. To understand the molecular pathogenesis of M. avium subspecies, we analyzed the genome contents of isolates collected from humans and domesticated or wildlife animals. Comparative genomic hybridizations indicated distinct lineages for each subspecies where the closest genomic relatedness existed between M. avium subsp. paratuberculosis isolates collected from human and clinical cow samples. Genomic islands (n = 24) comprising 846 kb were present in …

Collapsin Response Mediator Protein 1 Mediates Reelin Signaling In Cortical Neuronal Migration, Naoya Yamashita, Yutaka Uchida, Toshio Ohshima, Syu-Ichi Hirai, Fumio Nakamura, Masahiko Taniguchi, Katsuhiko Mikoshiba, Jerôme Honnorat, Pappachan Kolattukudy, Nicole Thomasset, Kohtaro Takei, Takuya Takahashi, Yoshio Goshima

Faculty Bibliography 2000s

Collapsin response mediator protein 1 ( CRMP1) is one of the CRMP family members that mediates signal transduction of axon guidance molecules. Here, we show evidence that CRMP1 is involved in Reelin ( Reln) signaling to regulate neuronal migration in the cerebral cortex. In crmp1(-/-) mice, radial migration of cortical neurons was retarded. This phenotype was not observed in the sema3A(-/-) and crmp1(+/+); sema3A(+/+) cortices. However, CRMP1 was colocalized with disabled- 1 ( Dab1), an adaptor protein in Reln signaling. In the Reln(rl/rl) cortex, CRMP1 and Dab1 were expressed at a higher level, yet tyrosine phosphorylated at a lower level. …

Biodiversity Of Sessile And Motile Macrofauna On Intertidal Oyster Reefs In Mosquito Lagoon, Florida, Michelle L. Boudreaux, Jennifer L. Stiner, Linda J. Walters

Faculty Bibliography 2000s

Our research focused on determining the diversity and abundance of sessile and motile macrofauna that use intertidal reefs of the eastern oyster Crassostrea virginica for feeding, settlement space or refuge in Mosquito Lagoon, Florida. Five replicate lift nets were deployed at six sites (three impacted reefs with seaward margins of disarticulated shells, three reference reefs Without dead margins) to determine the species composition and numbers present on these reef types. All nets were deployed intertidally on backreef areas on living oyster reefs, just above mean low water. One and a half liters of live oysters and oyster shells were placed …

Characterization Of The Ifn-Gamma T-Cell Responses To Immediate Early Antigens In Humans With Genital Herpes, Ralph P. Braun, Lendon G. Payne, Lichun Dong

Faculty Bibliography 2000s

Background: The IFN-gamma ELISPOT assay has been used to examine the T-cell repertoire for many disease states in humans but, as yet, not genital herpes. Using overlapping synthetic peptide libraries, an IFN-gamma ELISPOT assay was established that could measure CD4 and CD8 T-cell responses to HSV-2 antigens in patients with genital herpes. Results: In unexpanded T-cells isolated from peripheral blood, CD4 responses were readily measured against four immediate early antigens (ICP0, ICP4, ICP22 and ICP27), VP22 and gD. The CD4 responses were characterized by a low number of positive cells which produced large ELISPOTs. CD4 responses had a broad specificity …

Mitochondrial Uncoupling Protein-4 Regulates Calcium Homeostasis And Sensitivity To Store Depletion-Induced Apoptosis In Neural Cells, Sic. L. Chan, Dong Liu, George A. Kyriazis, Pamela Bagisyao, Xin Ouyang, Mark P. Mattson

Faculty Bibliography 2000s

An increase in the cytoplasmic-free Ca2+ concentration mediates cellular responses to environmental signals that influence a range of processes, including gene expression, motility, secretion of hormones and neurotransmitters, changes in energy metabolism, and apoptosis. Mitochondria play important roles in cellular Ca2+ homeostasis and signaling, but the roles of specific mitochondrial proteins in these processes are unknown. Uncoupling proteins (UCPs) are a family of proteins located in the inner mitochondrial membrane that can dissociate oxidative phosphorylation from respiration, thereby promoting heat production and decreasing oxyradical production. Here we show that UCP4, a neuronal UCP, influences store-operated Ca2+ entry, a process in …

Theta-Defensins Prevent Hiv-1 Env-Mediated Fusion By Binding Gp41 And Blocking 6-Helix Bundle Formation, Stephen A. Gallo, Wei Wang, Satinder S. Rawat, Grace Jung, Alan J. Waring, Alexander M. Cole, Hong Lu, Xuxia Yan, Norelle L. Daly, David J. Craik, Shibo Jiang, Robert I. Lehrer, Robert Blumenthal

Faculty Bibliography 2000s

Retrocyclin-1, a 0-defensin, protects target cells from human immunodeficiency virus, type 1 (HIV-1) by preventing viral entry. To delineate its mechanism, we conducted fusion assays between susceptible target cells and effector cells that expressed HIV-1 Env. Retrocyclin-1 (4 mu M) completely blocked fusion mediated by HIV-1 Envs that used CXCR4 or CCR5 but had little effect on cell fusion mediated by HIV-2 and simian immunodeficiency virus Envs. Retrocyclin-1 inhibited HIV-1 Env-mediated fusion without impairing the lateral mobility of CD4, and it inhibited the fusion of CD4-deficient cells with cells bearing CD4-independent HIV-1 Env. Thus, it could act without cross-linking membrane …

In Search Of Quorum Effects In Metacommunity Structure: Species Co-Occurrence Analyses, David G. Jenkins

Faculty Bibliography 2000s

Alternative models of community assembly emphasize regional, stochastic, dispersal-based processes or local, deterministic, niche-based processes. Community ecology's historical focus on local processes implicitly assumes that local processes surpass regional processes over time or across space to derive nonrandom metacommunity structure ( i.e., a quorum effect). Quorum effects are expected late in succession among nearby sites, whereas quorum effects are not expected early in succession among distant sites. I conducted a meta-analysis of zooplankton data sets encompassing time scales of one to thousands of years and spatial scales of < 1 m to thousands of kilometers. Species co-occurrence analyses statistically evaluated presence/absence patterns relative to random patterns obtained with Monte Carlo null models. A series of weighted analyses was conducted and alternative randomization algorithms and null models were evaluated. Most zooplankton metacommunities were randomly structured in unweighted analyses, and the distribution of significant structure did not follow quorum effect predictions. Weighted analyses ( e. g., by habitat area) revealed significant, nonrandom structure in most zooplankton metacommunities, but the distribution of significant structure still did not adhere well to quorum effect predictions. Finally, additional weighting for study scale (number of sites) nullified most significant area-weighted structure, and again, the distribution of significant structure did not follow quorum effect predictions. Overall, a quorum effect was not supported, perhaps related to zooplankton life histories and energetics and/ or the quorum effect itself. Results at the presence/absence level of resolution indicated that local processes did not generally override regional processes over time or across space to drive community structure. A full integration of dispersal- and niche-based concepts in metacommunity dynamics will be most fruitful for unraveling community assembly. Species co-occurrence analyses were scale dependent ( habitat area and study size). Future analyses should use weights for important factors ( e. g., habitat area), and meta-analyses should include study scale as an additional factor contributing to apparent patterns.

The Complete Chloroplast Genome Sequence Of Gossypium Hirsutum: Organization And Phylogenetic Relationships To Other Angiosperms, Seung-Bum Lee, Charalambos Kaittanis, Robert K. Jansen, Jessica B. Hostetler, Luke J. Tallon, Christopher D. Town, Hnery Daniell

Faculty Bibliography 2000s

Background: Cotton (Gossypium hirsutum) is the most important fiber crop grown in 90 countries. In 2004-2005, US farmers planted 79% of the 5.7-million hectares of nuclear transgenic cotton. Unfortunately, genetically modified cotton has the potential to hybridize with other cultivated and wild relatives, resulting in geographical restrictions to cultivation. However, chloroplast genetic engineering offers the possibility of containment because of maternal inheritance of transgenes. The complete chloroplast genome of cotton provides essential information required for genetic engineering. In addition, the sequence data were used to assess phylogenetic relationships among the major clades of rosids using cotton and 25 other completely …

Paradigms Of National Identity In Francisco De Quevedo's Poetry: Los(Des) Engaños De La Grandeza, Mark J. Mascia

Languages Faculty Publications

The purpose of this study is to examine the ways in which Quevedo explores Spanish national identity and to arrive at a conclusion as to what he had considered paradigmatic about being Spanish.

Liability, Regulation And Policy In Surgical Innovation: The Cutting Edge Of Research And Therapy, Anna C. Mastroianni

Health Matrix: The Journal of Law-Medicine

No abstract provided.

How Lidocaine Acts In Morphine Dependency, Behnaz Sedighi, Abbas Haghparast, Tajpary Klantary, Maryam Taieban

Neurosciences

OBJECTIVE: To study the effect of analgesia caused by a local anesthetic agent (Lidocaine) in morphine dependent and independent rats. METHODS: We carried out this experimental study in the Neuroscience Research Center of Kerman Medical University, Iran in 2003. We evaluated 2 groups of morphine dependent and independent rats. Morphine dependency was induced orally, and formalin was used as a noxious stimulus. The orofacial formalin test in rats is a valid and reliable model of nociception. The formalin test induces 2 distinct periods of nociception reaction, the first phase occurs in the first 3 minutes and the second phase 15-45 …

The Federal Criminal Code Is A Disgrace: Obstruction Statutes As Case Study, Julie R. O'Sullivan

Journal of Criminal Law and Criminology

No abstract provided.

Come Closer, Eli Marco Hall

Theses

This Culminating Project describes the design process from many different perspectives. It includes the designing of a book of poetry enabling me to apply design concepts.

Working as a graphic designer in the early twenty first century has provided opportunities to witness the effect that technology has had on the industry. Through the ongoing research, the change in roles of the modern graphic designer is established. Also during this time, print and digital media aggressively work toward a merger. When choosing to do a book for the Culminating Project, it was taken into consideration what the digital and print world …

A Procedural Learning Mechanism For Novel Skill Acquisition, S. Mello, U. Ramamurthy, A. Negatu, S. Franklin

CCRG Papers

No abstract provided.

Motivations, Values And Emotions: 3 Sides Of The Same Coin, S. Franklin, U. Ramamurthy

CCRG Papers

No abstract provided.

Lida: A Computational Model Of Global Workspace Theory And Developmental Learning, U. Ramamurthy, S. Mello, S. Franklin

CCRG Papers

No abstract provided.

An Evaluation Of The Effect Of Terrain Normalization On Classification Accuracy Of Landsat Etm+ Imagery, Jesse B. Bishop

Master's Theses and Capstones

More than 60% of land in New Zealand has been converted from native forests to residential areas, agriculture, or forest plantations. Settlers brought many species of plants and animals to New Zealand. Many native species were unable to protect themselves from these new predators, causing numerous extinctions. In light of this rapid decline in biodiversity, the New Zealand government has attempted to mitigate the destruction of endemic flora and fauna through both new environmental policies and intensive land management. Land management techniques include the restoration of developed land and the protection of remaining areas of native forest. Monitoring of restoration …

The Expression Of Heat Shock Protein 70, Heat Shock Protein 90 And Heat Shock Factor 1 Within The Bovine Midcycle Corpus Luteum After Stimulation With Temperature In Vitro And Pgf2alpha In Vitro And In Vivo, Melissa N. Kopka

Master's Theses and Capstones

Environmental and biological stressors affect cellular function. Heat shock proteins (HSPs) are molecular chaperones that safeguard cells against these stressors by protecting endogenous proteins from degradation. Heat shock factors (HSF) are their transcriptional regulators. In the present study, we evaluated whether heat (Experiment 1) or prostaglandin F2alpha (PGF2alpha; Experiments 2 and 3) induces the expression of HSP 70, HSP 90 and HSF1 in the bovine corpus luteum (CL). In summary, in vitro heat treatment elicited a later response in luteal cells by increasing HSP 70 expression at 24 hrs, while in vivo PGF2alpha treatment leads to an earlier response by …

Retinal Tracking System Design For Smart Flashblindness Non Lethal Weapon, Gregory T. Wolff

Master's Theses and Capstones

Development of a non lethal method of providing incapacitation of human subjects is of primary importance in both law enforcement and military fields. The proposed Smart White Light Dazzler is such a device that induces flashblindness in the subject using a flash of white light. It is intended to automatically track the retina of a human subject, and then make adjustments to the power of the flash based on factors that affect the susceptibility of the eye. Temporary blindness is induced in the subject without risking permanent tissue damage.

The primary contribution of this research is the design of a …

Setting The Pole: Critical Factors In Nascar's Success, Matthew N. Maisano

Seton Hall University Dissertations and Theses (ETDs)

This study examines the critical factors that make NASCAR so successful. Now known as the fastest growing sport, NASCAR started out as a limited funding, dirt track racing sport to a multimillion dollar, super speedway sport. Just like every sport, NASCAR has gone through many changes and this study will further discuss how it went from a sport to a business and then to entertainment and now a lifestyle. In the beginning NASCAR drivers were renegades with ties to moonshining; today drivers are considered role models and are active in Pop Culture. Constant changes in the sport have allowed it …

An Exonic Splicing Silencer Is Involved In The Regulated Splicing Of Glucose 6-Phosphate Dehydrogenase Mrna, Wioletta Szeszel-Fedorowicz, Indrani Talukdar, Brian N. Griffith, Callee M. Walsh, Lisa M. Salati

Faculty & Staff Scholarship

No abstract provided.

'Olah: The Rhetoric Of Burnt Offerings, James W. Watts

Religion - All Scholarship

The ‘olah offering receives pride of place in most lists of sacrifices in the Hebrew Bible, including the ritual rules of Leviticus. Its prominence in these texts suggests that the writers expected its mention to have an effect on their audience. This rhetorical effect must be evaluated and understood before the references to the `olah can be used to reconstruct ancient religious practices reliably. A comparative analysis of the rhetoric about the `olah suggests that its priority burnished the image of priests as devoted selflessly to divine worship and drew attention away from their economic interests in the sacrificial system …

A Therapeutic Jurisprudential Approach To Guardianship Of Persons With Mild Cognitive Impairment, Patricia C. Mcmanus

Seton Hall Law Review

No abstract provided.

Pharmacological Characterization Of Brain Angiotensin Ii Receptor Function And Ligand Binding, Surinder Kaur

Electronic Theses and Dissertations

No abstract provided.

Constructing A New Game: J.K. Rowling’S Quidditch And Global Kid Culture, Terri Toles Patkin

Reconstruction: Studies in Contemporary Culture

As part of her wildly popular Harry Potter fantasy series, author J.K. Rowling created a setting of magical characters to populate magical towns, perform magical jobs, purchase magical products -- and play a magical game called Quidditch. This fast-paced team sport played on broomsticks is both a participant and a spectator sport, and forms a nucleus of leisure activity and discourse in the Harry Potter books. From preschoolers to professionals, wizards in Rowling's world incorporate this virtual sport into their everyday lives. Rowling's development of Quidditch -– some have suggested that Rowling's intense dislike of school hockey practices sparked the …

You Need Love And Friendship For This Mission!: The Final Fantasy Series As Social Education For Japanese Youth, Ben Hourigan

Reconstruction: Studies in Contemporary Culture

From the mid to late 1990s, popular and academic discourse on video games, particularly in the United States, has focused on their supposed capacity to engender violent and otherwise anti-social behavior in youth. Much like ludologists' recent claims that video games are not a narrative medium, at least one reason for this is that commentators have too narrow an understanding of the diverse range of games that exist. In addition to this, game demonizers have demonstrated an inadequate understanding of the ways games are related to real life. In order to combat these misunderstandings, this chapter analyzes the role of …

Little Gods And Little Heroes: Hyperreal Ethics In God Games And Online Gaming, G. Christopher Williams

Reconstruction: Studies in Contemporary Culture

Media critics, notably Jean Baudrillard, have suggested that the environment of simulations are to be defined as something more real than the real, or, in his terms, hyperreal. If hyperrealities are indeed the way that we can define the environment of multimedia technologies like video games, what sorts of ethical considerations should exist in a place that gamers conceive as more real than the real? This essay considers what ethical considerations are worth contemplating as gamers begin to take on the roles of gods and heroes in multiplayer video games. The little gods and heroes whose identities gamers have begun …

Cyanidin-3-Glucoside, A Natural Product Derived From Blackberry, Exhibits Chemopreventive And Chemotherapeutic Activity, Min Ding, Rentian Feng, Shiow Y. Wang, Linda Bowman, Yongju Lu, Yong Qian, Vincent Castranova, Bing-Hua Jiang, Xianglin Shi

Faculty & Staff Scholarship

Epidemiological data suggest that consumption of fruits and vegetables has been associated with a lower incidence of cancer. Cyanidin-3-glucoside (C3G), a compound found in blackberry and other food products, was shown to possess chemopreventive and chemotherapeutic activity in the present study. In cultured JB6 cells, C3G was able to scavenge ultraviolet B-induced *OH and O2-* radicals. In vivo studies indicated that C3G treatment decreased the number of non-malignant and malignant skin tumors per mouse induced by 12-O-tetradecanolyphorbol-13-acetate (TPA) in 7,12-dimethylbenz[a]anthracene-initiated mouse skin. Pretreatment of JB6 cells with C3G inhibited UVB- and TPA-induced transactivation of NF-kappaB and AP-1 and expression of …

More Than Girlfriends, Geekettes, And Gladiatixes: Women, Feminism, And Fantasy Role-Playing Games, Rebecca Borah, Inez Schaechterle

Reconstruction: Studies in Contemporary Culture

[First paragraph]

The only function women have in D&D is to serve cookies. Gary Gygax, the most well known of the creators of Dungeons & Dragons, claims he did not make this remark (Gel214th). That he did or not is perhaps, some thirty years after the introduction of the first fantasy role-playing game, of concern only to him. The problematic position of women players is a secure feature of the RPG zeitgeist. While few male gamers may openly challenge the assumption that women can or should play fantasy role games such as Dungeons & Dragons, the function of …