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Seasonal Migration And Home Ranges Of Female Elk In The Black Hills Of South Dakota And Wyoming, Lakhdar Benkobi, Mark A. Rumble, Cynthia H. Stubblefield, R. Scott Gamo, Joshua J. Millspaugh

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Understanding the movement and dispersion patterns of elk (Cervus elaphus) on public lands and the underlying factors that affect each will facilitate elk management and help resolve conflicts between management that benefit elk and other uses of land resources. Consequently, there is a need to identify and examine the movement and dispersion patterns of elk in the Black Hills of South Dakota and Wyoming. Our study quantified seasonal movements, determined home ranges of female elk in two areas of the Black Hills, and examined underlying factors associated with each. Elk in the northern area did not demonstrate seasonal …

Science Priorities For Reducing The Threat Of Invasive Species To Sustainable Forestry, Elizabeth A. Chornesky, Ann M. Bartuska, Gregory H. Aplet, Kerry O. Britton, Jane Cummings-Carlson, Frank W. Davis, Jessica Eskow, Doria R. Gordon, Kurt W. Gottschalk, Robert A. Haack, Andrew J. Hansen, Richard N. Mack, Frank J. Rahel, Margaret A. Shannon, Lisa A. Wainger, T. Bently Wigley

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Invasive species pose a major, yet poorly addressed, threat to sustainable forestry. Here we set forth an interdisciplinary science strategy of research, development, and applications to reduce this threat. To spur action by public and private entities that too often are slow, reluctant, or unable to act, we recommend (a) better integrating invasive species into sustainable forestry frameworks such as the Montréal Process and forest certification programs; (b) developing improved cost estimates to inform choices about international trade and pest suppression efforts; and (c) building distributed information systems that deliver information on risks, identification, and response strategies. To enhance the …

A Retrospective Analysis Of Known And Potential Risks Associated With Exotic Toadflax-Feeding Insects, Sharlene E. Sing, Robert K.D. Peterson, David K. Weaver, Richard W. Hansen, George P. Markin

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

To date, eight exotic toadflax-feeding insect species have been accidentally or intentionally introduced to North America. Reports on their establishment and impact have been recorded for more than 60 years. Environmental risks linked to biological control of toadflax were identified in terms of host resources and undesirable impacts on the target species through the critical review of this record. Data gaps revealed during this retrospective analysis are addressed through suggestions for future research and associated experimental methodologies. Known and potential impacts of toadflax-feeding insects on both invasive toadflax and non-target species are examined. Recent programmatic demands for demonstrated agent efficacy …

Environmental Quality Incentives Program Contributions To Fish And Wildlife Conservation, Mark W. Berkland, Charles A. Rewa

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

The Environmental Quality Incentives Program (EQIP) is a voluntary program whereby the U.S. Department of Agriculture provides technical and financial assistance to active farmers and ranchers to address natural resource concerns such as soil conservation, water quality and quantity, nutrient management, and fish and wildlife habitat. The Natural Resources Conservation Service (NRCS) is working with these landowners to maximize the environmental benefits gained for the expenditures made in the program. Funding has expanded significantly under the 2002 Farm Bill, with the amount of annual funding authorized reaching $1.3 billion by fiscal year 2007. The EQIP has been used to implement …

Fish And Wildlife Benefits Of The Wildlife Habitat Incentives Program, Randall L. Gray, Sally J. Benjamin, Charles A. Rewa

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Th e Wildlife Habitat Incentives Program (WHIP) is a voluntary program that encourages the establishment and enhancement of a wide variety of fish and wildlife habitats of national, state, tribal, or local significance. Through voluntary agreements, the Natural Resources Conservation Service (NRCS) provides financial and technical assistance to participants who installed habitat restoration and management practices. Since 1998, nearly $150 million has been dedicated to the program and over 2.8 million acres involving over 18,000 contracts have been enrolled. A wide range of habitat-enhancement actions are cost-shared through the program, affecting hundreds of target and non-target species. While few quantitative …

Wildlife Benefi Ts Of The Wetlands Reserve Program, Charles A. Rewa

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Since its initial authorization in 1990, more than 1.6 million acres of primarily drained or degraded wetlands on agricultural lands have been enrolled in the U.S. Department of Agriculture’s (USDA) Wetlands Reserve Program (WRP). The Natural Resources Conservation Service (NRCS) and its partners are working with landowners to restore these lands to ecologically productive wetland and upland buffer habitats. Numerous studies have documented the value of restored and created wetlands to fish and wildlife resources. However, few objective studies have been completed that document fish and wildlife response to wetlands enrolled in and restored through WRP. Preliminary results of some …

Biology And Biological Control Of Knapweed, Linda M. Wilson, Carol Bell Randall

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

The knapweeds comprise a diverse complex of species that predominantly infest rangelands in the western United States and Canada. This manual considers the biological control of six species of knapweeds: 1) spotted knapweed, 2) diffuse knapweed, 3) squarrose knapweed, 4) meadow knapweed, 5) black knapweed, and 6) brown knapweed. Spotted knapweed, Centaurea stoebe, is perhaps the most widespread species, followed in abundance by diffuse knapweed, C. diffusa. A third species, squarrose knapweed, C. virgata var. squarrosa, has a more limited distribution in the West. Three less widespread species, meadow (C. pratensis), brown (C. jacea …

Replication And Encapsidation Of Papillomaviruses In Saccharomyces Cerevisiae, Peter C. Angeletti

Nebraska Center for Virology: Faculty Publications

Improvements in methodologies to recapitulate and study particular biological functions of the

papillomavirus life cycle have led to great advances in our knowledge of these viruses. Described in

this chapter are techniques that allow low-copy and high-copy replication of full-length human

papillomavirus (HPV) genomes, as well as assembly of virus-like particles, in Saccharomyces

cerevisiae (yeast). This system has several distinct advantages that make it an attractive complement

to the well-established raft-culturing system. First, yeast are inexpensive, rapid, and simple to culture

in the lab. Second, they provide an ever-widening array of genetic tools to analyze HPV functions

—most recently notable, …

Antigenicity And Immunogenicity Of A Synthetic Human Immunodeficiency Virus Type 1 Group M Consensus Envelope Glycoprotein, Feng Gao, Eric A. Weaver, Zhongjing Lu, Yingying Li, Hua-Xin Liao, Benjiang Ma, S. Munir Alam, Richard M. Scearce, Laura L. Sutherland, Jae-Sung Yu, Julie M. Decker, George M. Shaw, David C. Montefiori, Bette T. Korber, Beatrice H. Hahn, Barton F. Haynes

Nebraska Center for Virology: Faculty Publications

Genetic variation of human immunodeficiency virus (HIV-1) represents a major obstacle for AIDS vaccine development. To decrease the genetic distances between candidate immunogens and field virus strains, we have designed and synthesized an artificial group M consensus env gene (CON6 gene) to be equidistant from contemporary HIV-1 subtypes and recombinants. This novel envelope gene expresses a glycoprotein that binds soluble CD4, utilizes CCR5 but not CXCR4 as a coreceptor, and mediates HIV-1 entry. Key linear, conformational, and glycan-dependent monoclonal antibody epitopes are preserved in CON6, and the glycoprotein is recognized equally well by sera from individuals infected with different HIV-1 …

Antigenicity And Immunogenicity Of A Synthetic Human Immunodeficiency Virus Type 1 Group M Consensus Envelope Glycoprotein, Feng Gao, Eric A. Weaver, Zhongjing Lu, Yingying Li, Hua-Xin Liao, Benjiang Ma, S. Munir Alam, Richard M. Scearce, Laura L. Sutherland, Jae-Sung Yu, Julie M. Decker, George M. Shaw, David C. Montefiori, Bette T. Korber, Beatrice H. Hahn, Barton F. Haynes

Nebraska Center for Virology: Faculty Publications

Genetic variation of human immunodeficiency virus (HIV-1) represents a major obstacle for AIDS vaccine development. To decrease the genetic distances between candidate immunogens and field virus strains, we have designed and synthesized an artificial group M consensus env gene (CON6 gene) to be equidistant from contemporary HIV-1 subtypes and recombinants. This novel envelope gene expresses a glycoprotein that binds soluble CD4, utilizes CCR5 but not CXCR4 as a coreceptor, and mediates HIV-1 entry. Key linear, conformational, and glycan-dependent monoclonal antibody epitopes are preserved in CON6, and the glycoprotein is recognized equally well by sera from individuals infected with different HIV-1 …

Biological Opinion On The Operation Of The Missouri River Main Stem System, Missouri River Bank Stabilization And Navigation Project, And Operation Of The Kansas River Reservoir System

United States Army Corps of Engineers, Omaha District

The U.S. Army Corps of Engineers (Corps) prepares an annual report for interested parties in accordance with reporting requirements of the "Biological Opinion (BiOp) on the Operation of the Missouri River Main Stem System, Operation and Maintenance of the Missouri River Bank Stabilization and Navigation Project (BSNP), and Operation of the Kansas River Reservoir System" prepared by the U.S. Fish and Wildlife Service (Service) dated November 30,2000 and the Amendment, thereto, dated December 16,2003.

This annual report documents the Corps threatened and endangered species conservation activities to comply with the provisions of the 2000 BiOp and the 2003 Amendment to …

A Retrospective Perspective: Evaluating Population Changes By Repeating Historic Bird Surveys, Lawrence D. Igl, Douglas H. Johnson

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Acquiring an accurate picture of the changes in bird populations often involves a tradeoff between the time and effort required to complete the surveys and the number of years spent surveying the bird populations. An alternative approach to long-term monitoring efforts is to collect current data and contrast those with data collected earlier in a similar fashion on the same study site(s). To evaluate changes in bird populations, we repeated two extensive surveys, one in North Dakota (1967 vs. 1992-1993) and the other in the Platte River Valley of Nebraska (1979-1980 vs. 2001), where large areas of native vegetation had …

Maternal Yolk Steroids: A Potential Compensatory Mechanism For Red-Winged Blackbirds (Agelaius Phoeniceus) In North Dakota, Rachel M. Bush, Mark E. Clark, Wendy L. Reed, George M. Linz

United States Department of Agriculture Wildlife Services: Staff Publications

Many of the suggested management techniques directed at reducing blackbird breeding populations fail to incorporate the underlying mechanisms regulating populations. Theoretically, removal of individuals from the breeding population should lower the breeding densities and presumably reduce recruitment. However, compensatory responses might occur with decreased breeding densities, but no empirical data are available to test this hypothesis. Much of the underlying compensatory theory is based on differential allocation of resources to reproduction vs. self maintenance at different breeding densities, mainly in the form of depensatory effects of resource limitation on growth and survival. However, as the breeding density in an area …

Blackbird Use And Damage Of Wildlife Conservation Sunflower Plots, Jonathan M. Raetzman, George M. Linz, William J. Bleier

United States Department of Agriculture Wildlife Services: Staff Publications

Annual blackbird damage to sunflower is valued at $5 to $10 million. Perhaps more importantly, the National Sunflower Association has identified blackbirds as the key reason for growers to abandon sunflower in areas of prime production. North Dakota and South Dakota together produce approximately 70% of sunflower grown in the United States. This crop adds $906 million to the regional economy. To date, cost effective and environmentally safe methods to reduce bird damage to sunflower remain elusive. In the 1980’s, scientists from the National Wildlife Research Center (NWRC) showed that “decoy” sunflower plots can greatly reduce bird damage to local …

Wildlife Disease Research At The Aphis National Wildlife Research Center, R. G. Mclean, L. Clark, M. R. Dunbar, K. C. Vercauteren, T. A. Campbell

United States Department of Agriculture Wildlife Services: Staff Publications

Research on wildlife diseases at the newly formed Wildlife Disease Program (WDP) at the United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service (APHIS), Wildlife Services (WS), National Wildlife Research Center (NWRC) concentrates on wildlife diseases of importance to domestic animal and human health. We are conducting studies on wildlife rabies, bovine tuberculosis (TB), chronic wasting disease (CWD), West Nile virus (WNV), pathogenic bacteria of birds, and pseudorabies (PR). The goal of the research is to develop innovative methods for surveillance, intervention, prevention, and control of these diseases.

Historic And Current Status Of The American White Pelican Breeding At Chase Lake National Wildlife Refuge, North Dakota, Marsha A. Sovada, D. Tommy King, Michael Erickson, Cheryl Gray

United States Department of Agriculture Wildlife Services: Staff Publications

Chase Lake National Wildlife Refuge currently supports the largest breeding colony of the American White Pelican ( Pelecanus erythrorhynchos ) in North America. The first written account of the colony’s status was in 1905, when approximately 500 pelicans nested on two islands in Chase Lake. The colony grew to nearly 34,000 breeding birds by 2002. Surveys of breeding pelicans in the colony were sporadic from 1905 until 1972. Because the availability of foraging areas affects recruitment, pond counts from south-central North Dakota were used as an index of the relative availability of foraging areas. Pond counts varied widely in the …

Interactions Between The American White Pelican And Aquaculture In The Southeastern United States: An Overview, D. Tommy King

United States Department of Agriculture Wildlife Services: Staff Publications

The eastern metapopulation of the American White Pelican ( Pelecanus erythrorhynchos ) breeds mainly in the Northern Great Plains, migrates through the Great Plains and along the Mississippi River and winters in the lower Mississippi River Valley and along the Gulf of Mexico. The production of farm-raised Channel Catfish ( Ictalurus punctatus ) in the southeastern United States has increased dramatically in the last 25 years. In 1990, U.S. Department of Agriculture Wildlife Services offices in Arkansas, Louisiana and Mississippi began receiving complaints concerning the foraging of pelicans in commercial Channel Catfish ponds. Because of the relatively shallow depth and …

Recent Population Status Of The American White Pelican: A Continental Perspective, D. Tommy King, Daniel W. Anderson

United States Department of Agriculture Wildlife Services: Staff Publications

Breeding bird surveys were conducted at most American White Pelican ( Pelecanus erythrorhynchos ) colonies in North America from 1979-81 and the number of American White Pelicans was estimated at >109,000 breeding individuals in 55 colonies. Although many pelican colonies have not been surveyed since the early 1980s, we present the most current survey numbers available for North American colonies. Using available data (1998-2001) and the North American Continental Divide to delineate eastern from western metapopulations, 27 colonies and 48,240 nests in the East and 15 colonies and 18,790 nests in the West were documented, giving a total of about …

Introduction: Biology And Conservation Of The American White Pelican, Daniel W. Anderson, D. Tommy King

United States Department of Agriculture Wildlife Services: Staff Publications

Two separate, large-scale management units for the American White Pelican ( Pelecanus erythrorhynchos ) in North America are proposed: the eastern and western metapopulations, separated by the North American Continental Divide. The populations on either side of this barrier are subject to contrasting ecological conditions, movement patterns suggest strong geographic separation and each is likely demographically distinct. Subdivisions within these demographic units need to be more precisely defined in the future. Yet, because of the highly colonial nesting habits of the American White Pelican, conservation can still be effective if directed toward separate breeding colonies. Our intent is to provide …

Developing Relative Abundance Techniques (Rats) For Monitoring Rodent Populations, Desely A. Whisson, Richard M. Engeman, Kellie Collins

United States Department of Agriculture Wildlife Services: Staff Publications

Accurate density estimates of rodents are frequently difficult or cost-prohibitive to determine. Thus, a number of techniques (track plates, monitoring blocks, chew cards/sticks, apple slice index, trapping) are often used to index rodent populations. Theoretically, the sensitivity of these indexing techniques could be improved if they applied continuous measurements rather than simple binary measurements (presence/absence). Development of a relative abundance technique that is simple to apply and sensitive to changes in population density is critical for the operational management of rodent pests. In addition to providing a quantitative measure of abundance with statistical parameters, an indexing technique that does not …

The Impact Of Disease In The American White Pelican In North America, Tonie Rocke, Kathryn Converse, Carol Meteyer, Bob Mclean

United States Department of Agriculture Wildlife Services: Staff Publications

Records of reported die-offs of the American White Pelican ( Pelicanus erythrorhynchos ) held by the U.S. Geological Survey National Wildlife Health Center from 1978 through 2003 indicate that type C botulism (caused by Clostridium botulinum ) was the major cause of mortality. In 1996, over 15,000 birds, including 8,500 American White Pelicans, were estimated to have died from type C botulism at the Salton Sea in California. This was the largest documented die-off of any pelican species and was estimated to represent 15-20% of the western metapopulation. This event was also notable in that it was the first time …

Husbandry Of Wild-Caught Greater Sage-Grouse, Paul Oesterle, Robert Mclean, Michael Dunbar, Larry Clark

United States Department of Agriculture Wildlife Services: Staff Publications

This study reports the first successful husbandry and breeding in captivity of wild-caught greater sage-grouse (Ccntroccrcus urophasianus). In October 2003, 2 1 hatch-year greater sage-grouse were trapped in northwestern Nevada and transported to Fort Collins, Colorado. We held grouse in pens at the United States Department of Agriculture's National Wildlife Research Center for 8 months. We offered a varied diet, including native food items such as sagebrush (Arternisia tridentata and A. tripartita) and yarrow (Achilles millefolium). We housed grouse in a large flight pen and allowed to them freerange as one flock. Mortdlity rate was 16.7%. Several of the grouse …

From The Field: Field Searches Versus Vaginal Implant Translocating Elk Calves, Nathan W. Seward, David S. Maehr, Jonathan W. Gassett, John J. Cox, Jeffrey L. Larkin

United States Department of Agriculture Wildlife Services: Staff Publications

We compared the utility of vaginal implant transmitters (VITs) and field searches for locating elk (Cervus elaphus) calves in southeastern Kentucky during the spring of 2001. Retention of VITs among 40 adult females ranged from 1–276 days (x - =61.6±8.4 [SE] days). Approximately half of the transmitters (n=19) were expelled prematurely or malfunctioned. Two of 37 (5%) transmitters in translocated females performed as designed and led to calf capture. We captured more calves (n=9 in 2001; n=16 in 2002) by searching areas where elk exhibited parturition behavior. We suggest that manufacturers refine the current VIT design to increase its application …

Causes Of Wolf Depredation Increase In Minnesota From 1979-1998, Elizabeth K. Harper, William J. Paul, L. David Mech

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Wolf (Canis lupus) depredations on livestock in Minnesota have been increasing over the last 20 years. A major explanation cited for this increase is wolf range expansion, but no studies have tested this explanation. Additional reasons could include 1) wolf colonization of new areas within long-existing wolf range, 2) learning by wolves in established range, and 3) increased wolf density. We did not assess increasing wolf density as a factor because estimated wolf density in Minnesota has not increased. To assess how each of the other factors might have affected depredations, we created and analyzed a database of …

Chlorovirus: A Genus Of Phycodnaviridae That Infects Certain Chlorella-Like Green Algae, Ming Kang, David Dunigan, James L. Van Etten

Department of Plant Pathology: Faculty Publications

Taxonomy: Chlorella viruses are assigned to the family Phycodnaviridae, genus Chlorovirus, and are divided into three species: Chlorella NC64A viruses, Chlorella Pbi viruses and Hydra viridis Chlorella viruses. Chlorella viruses are large, icosahedral, plaque-forming, dsDNA viruses that infect certain unicellular, chlorella-like green algae. The type member is Paramecium bursaria chlorella virus 1 (PBCV-1).

Physical properties: Chlorella virus particles are large (molecular weight ~1 × 10⁹ Da) and complex. The virion of PBCV-1 contains more than 100 different proteins; the major capsid protein, Vp54, comprises ~40% of the virus protein. Cryoelectron microscopy and three-dimensional image reconstruction of PBCV-1 virions …

Proline Suppresses Apoptosis In The Fungal Pathogen Colletotrichum Trifolii, Changbin Chen, Martin B. Dickman

Department of Plant Pathology: Faculty Publications

The role of reactive oxygen species (ROS) in cell communication, control of gene expression, and oxygen sensing is well established. Inappropriate regulation of ROS levels can damage cells, resulting in a diseased state. In Colletotrichum trifolii, a fungal pathogen of alfalfa, the mutationally activated oncogenic fungal Ras (DARas) elevates levels of ROS, causing abnormal fungal growth and development and eventual apoptotic-like cell death but only when grown under nutrient-limiting conditions. Remarkably, restoration to the wild-type phenotype requires only proline. Here, we describe a generally unrecognized function of proline: its ability to function as a potent antioxidant and inhibitor of …

Chlorella Virus Marburg Topoisomerase Ii: High Dna Cleavage Activity As A Characteristic Of Chlorella Virus Type Ii Enzymes, Jennifer S. Dickey, Tae-Jin Choi, James L. Van Etten, Neil Osheroff

Department of Plant Pathology: Faculty Publications

Although the formation of a covalent enzyme-cleaved DNA complex is a prerequisite for the essential functions of topoisomerase II, this reaction intermediate has the potential to destabilize the genome. Consequently, all known eukaryotic type II enzymes maintain this complex at a low steady-state level. Recently, however, a novel topoisomerase II was discovered in Paramecium bursaria chlorella virus-1 (PBCV-1) that has an exceptionally high DNA cleavage activity [Fortune et al. (2001) J. Biol. Chem. 276, 24401-24408]. If robust DNA cleavage is critical to the physiological functions of chlorella virus topoisomerase II, then this remarkable characteristic should be …

Role Of The Hypervariable Hinge Region Of Phosphoprotein P Of Vesicular Stomatitis Virus In Viral Rna Synthesis And Assembly Of Infectious Virus Particles, Subash C. Das, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

The phosphoprotein (P protein) of vesicular stomatitis virus (VSV) is an essential subunit of the viral RNAdependent RNA polymerase and has multiple functions residing in its different domains. In the present study, we examined the role of the hypervariable hinge region of P protein in viral RNA synthesis and recovery of infectious VSV by using transposon-mediated insertion mutagenesis and deletion mutagenesis. We observed that insertions of 19-amino-acid linker sequences at various positions within this region affected replication and transcription functions of the P protein to various degrees. Interestingly, one insertion mutant was completely defective in both transcription and replication. Using …

Genome Of Deerpox Virus, C. L. Afonso, Gustavo A. Delhon, E. R. Tuhlman, Z. Lu, A. Zsak, V. M. Becerra, L. Zsak, G. F. Kutish, D. L. Rock

Nebraska Center for Virology: Faculty Publications

Deerpox virus (DPV), an uncharacterized and unclassified member of the Poxviridae, has been isolated from North American free-ranging mule deer (Odocoileus hemionus) exhibiting mucocutaneous disease. Here we report the genomic sequence and comparative analysis of two pathogenic DPV isolates, W-848-83 (W83) and W-1170-84 (W84). The W83 and W84 genomes are 166 and 170 kbp, containing 169 and 170 putative genes, respectively. Nucleotide identity between DPVs is 95% over the central 157 kbp. W83 and W84 share similar gene orders and code for similar replicative, structural, virulence, and host range functions. DPV open reading frames (ORFs) with putative …

Evolution Of Subtype C Hiv-1 Env In A Slowly Progressing Zambian Infant, Hong Zhang, Federico Hoffmann, Jun He, Xiang He, Chipepo Kankasa, Ruth Ruprecht, John T. West, Guillermo Orti, Charles Wood

Nebraska Center for Virology: Faculty Publications

Background: Given the high prevalence of mother to child infection, the development of a better understanding of African subtype C HIV-1 transmission and natural evolution is of significant importance. In this study, we genotypically and phenotypically characterized subtype C viruses isolated over a 67-month follow-up period from an in utero-infected Zambian infant. Changes in genotype and phenotype were correlated to alterations of the host humoral immune response.

Results: A comparison of baseline maternal and infant samples indicated that the infant sequences are monophyletic and contain a fraction of the diversity observed in the mother. This finding suggests that selective …