Digital Commons Network^™

Design By Nature, Timothy Shanahan

Philosophy Faculty Works

No abstract provided.

Expression Of Mslec1 Transgenes In Alfalfa Plants Causes Symbiotic Abnormalities, Nancy Fujishige

Biology Faculty Works

Legume lectins have been proposed to have important symbiotic roles during Rhizobium-legume symbioses. To test this hypothesis, the symbiotic responses of transgenic alfalfa plants that express a portion of the putative alfalfa lectin gene MsLEC1 or MsLEC2 in either the antisense or sense orientation were analyzed following inoculation with wild-type Sinorhizobium meliloti 1021. MsLEC1-antisense (LEC1AS) plants were stunted, exhibited hypernodulation, and developed not only abnormally large nodules but also numerous small nodules, both of which senesced prematurely. MsLEC2-antisense plants were intermediate in growth and nodule number compared with LEC1AS and vector control plants. The symbiotic abnormalities of MsLEC1-sense transgene plants …

The Debate Over Genetically Modified Crops In The United States: Reassessment Of Notions Of Harm, Difference, And Choice, Matthew Rich

Case Western Reserve Law Review

No abstract provided.

Development And Validation Of The Proactive Health Lifestyle Measures, Norma Jean Perez

Open Access Dissertations

This research project targets the three leading causes of death in the U.S., cardiovascular disease, cancer, and stroke through the successful development and validation of six culturally sensitive Proactive Healthy Lifestyle measures (Stage of Change, Decisional Balance, Self-Efficacy, Skills, Relapse, and Processes of Change) targeting diet, exercise, and smoking. 574 college students were recruited and completed self-report surveys. Data from 418 non-Hispanics was used to develop (N = 209) and validate (N = 209) each of the measures. Data from 155 Hispanics was used to further successfully validate the measures, and explore differences in this group with the non-Hispanic group. …

Evaluation Of The Hayes E. Willis Health Center In South Richmond -- Has It Served Its Targeted Population?, Shannon Nicole Shaw

Theses and Dissertations

Objective: The Hayes E. Willis Health Center (HWHC) was opened in a neighborhood of South Richmond in October 1993 to remove the barriers that prevented South Richmond residents from accessing primary healthcare services. The major objective of this study was to determine the effectiveness of the HWHC in providing primary care to the South Richmond Community deemed to be in need. An additional objective was to describe the changes in characteristics of clinic users and illnesses seen over time.Methods: Patient data was compared from the HWHC�s first full year of operation in which patient data was collected (1995) to the …

Antioxidants As Risk Factors For Gingival Bleeding, Hee-Jeong Bahng

Theses and Dissertations

Background: Studies of gingival bleeding and the effects of antioxidants on extracellular matrix and immunologic and inflammatory responses provide a rationale for hypothesizing that antioxidants reduce the risk for gingival bleeding.Methods: This study evaluated the role of antioxidants as contributing risk factors for gingival bleeding utilizing the Third National Health and Nutrition Examination Survey (NAHNES III). A sample of 18,825 adults (20 to ≥ 90 years of age), with dental measurement and assessment of serum levels of antioxidants were included in the study. Gingival bleeding was defined as those who had more than 30 percent of gingival bleeding in 28 …

A Continuing Whimsical Search For The True Meaning Of The Term “Product” In Products Liability Litigation, Charles E. Cantú

Faculty Articles

More than a decade has elapsed since an initial attempt was made to discern the true meaning of the term product in products liability litigation. At the time, a brief history of events leading up to the adoption of Section 402A of the Restatement (Second) of Torts was outlined, and it was emphasized that what had at first seemed so simple subsequently proved to be somewhat complex.

An examination of cases involving the sales/service transaction, as well as those involving real estate, blood, electricity, component parts, water, computer software, and ideas, sometimes held that what was involved was a product. …

Multiple Domains Of The Co-Chaperone Hop Are Important For Hsp70 Binding, Gregory M. Nelson, Patricia E. Carrigan, Patricia J. Roberts, Jha'nae Stoffer, Daniel L. Riggs, David F. Smith

Faculty Publications

The Hop/Sti1 co-chaperone binds to both Hsp70 and Hsp90. Biochemical and co-crystallographic studies have suggested that the EEVD-containing C terminus of Hsp70 or Hsp90 binds specifically to one of the Hop tetratricopeptide repeat domains, TPR1 or TPR2a, respectively. Mutational analyses of Hsp70 and Hop were undertaken to better characterize interactions between the C terminus of Hsp70 and Hop domains. Surprisingly, truncation of EEVD plus as many as 34 additional amino acids from the Hsp70 C terminus did not reduce the ability of Hsp70 mutants to co-immunoprecipitate with Hop, although further truncation eliminated Hop binding. Hop point mutations targeting a carboxylate …

Effects Of Low Tide Rainfall On Intertidal Zone Material Cycling, Raymond Torres, Miguel A. Goni, George Voulgaris, Charles R. Lovell, James T. Morris

Faculty Publications

Sediment transport by rainfall-runoff processes is well documented for terrestrial landscapes but few studies have focused on rainfall-runoff effects in intertidal areas. Here we present geochemical analyses performed on sediment samples collected during low tide irrigation experiments, and tidal channel turbidity measurements taken during natural rainfall over North Inlet Marsh, South Carolina. Order of magnitude approximations indicate that a single 10 minute storm may entrain 8-15% of the local annual average sediment accumulation. This rainfall-entrained material is enriched in organic nitrogen and marine algal matter, and therefore of high nutritional quality.

"Dawn And Doom Was In The Branches": Nature, Vodou And The Pastoral Impulse In Their Eyes Were Watching God, Christopher Rieger

The Marjorie Kinnan Rawlings Journal of Florida Literature

No abstract provided.

In Praise Of A Skeletal Apa: Judicial Discretion, Remedies For Agency Inaction And Apa Amendment, William D. Araiza

Faculty Scholarship

No abstract provided.

Considering Physical Inactivity In Relation To Obesity, Janet C. Struber

Internet Journal of Allied Health Sciences and Practice

Physical inactivity and obesity contribute enormously to the current burden of disease in Australia, as in all Western Countries. Although diet and exercise have been linked to health since antiquity, the connection has recently experienced a revival of interest. There is mounting evidence that the current prevalence of obesity is more closely related to decreases in energy expenditure than to the traditionally accepted imbalance between consumption and expenditure, and that physical activity is therefore crucial in maintaining weight loss. With overweight[1] and obesity[2] now the norm in Australia, physical inactivity is a major issue within and beyond the health sector, …

Strafford County 2003 Annual Report Of The Commissioners, Treasurer, Other County Officers And The Strafford County Delegation Strafford County, New Hampshire For The Year Ending December 31, 2003., Stafford County Representatives

Strafford County, NH Annual Reports

This is an annual report containing vital statistics for a county in the state of New Hampshire.

Lessons From The Interaction Of Biotechnology, Intellectual Property And World Needs, Charles C. Muscoplat

Minnesota Journal of Law, Science & Technology

No abstract provided.

Supercooling Differences In The Eastern Subterranean Termite (Isoptera: Rhinotermitidae), Brian J. Cabrera, Shripat T. Kamble

Department of Entomology: Faculty Publications

Supercooling points were determined for untreated field-collected and untreated laboratory-maintained Reticulitermes flavipes (Kollar) workers and soldiers. Workers treated with antibiotics or had their hindgut-protozoa removed by exposing them to oxygen under pressure to determine the effects of absence of the hindgut fauna on supercooling. Supercooling points were compared be-tween live and freshly killed workers to determine whether supercooling in this species might be simply due to the biochemical properties of body fluids. Laboratory-maintained workers were also subjected to desiccation, starvation, or atmospheric pressure to determine their effects on supercool-ing. Supercooling points were lowest for laboratory workers treated with antibiotics and …

Phospholipase A2 In Salivary Glands Isolated From Tobacco Hornworms, Manduca Sexta, Hasan Tunaz, David W. Stanley

Department of Entomology: Faculty Publications

We describe a phospholipase A₂ (PLA₂) associated with the salivary glands of tobacco hornworms, Manduca sexta. This enzyme is able to hydrolyze arachidonic acid from the sn-2 position of PLs. Addition of the calcium chelator, EGTA, or calcium, to the Tris reaction buffer impaired the PLA₂ activity, from which we infer the enzyme requires very low concentrations of calcium or perhaps other ions for optimal activity. PLA₂ activity was sensitive to protein concentration (highest activity at 25 μg protein per μl), reaction time (optimal at 30 min), buffer pH (optimal at pH 8–10), …

Guidelines For Managing Lesser Prairie-Chicken Populations And Their Habitats, Christian A. Hagen, Brent E. Jamison, Kenneth M. Giesen, Terry Z. Riley

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Lesser prairie-chicken (Tympanuchus paidicinctus) populations have declined by >90% since the 1800s. These declines have concerned both biologists and private conservation groups and led to a petition to list the lesser prairie-chicken as threatened under the Endangered Species Act. Most of the land in the current range of the lesser prairie-chicken is privately owned, and declines have been primarily attributed to anthropogenic factors. Conversion of native rangeland to cropland and excessive grazing have been implicated as leading causes in the species' decline. Periodic drought probably has exacerbated these problems. Little research on habitat requirements was conducted prior to …

Patterns Of Variation In Clutch Sizes In A Guild Of Temperate-Nesting Dabbling Ducks, Gary Krapu, Ronald Reynolds, Glen Sargeant, Randy Renner

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

We investigated patterns and causes of variation in clutch sizes in a guild of five species of temperate-nesting dabbling ducks (Mallard [Anas platyrhynchos], Northern Pintail [“pintail,” A. acuta], Gadwall [A. strepera], Blue-winged Teal [“teal,” A. discors], and Northern Shoveler [“shoveler,” A. clypeata]) during 1993–1995 in the Prairie Pothole Region of midcontinental North America. Clutch sizes (mean ± SE) were largest for teal (10.80 ± 0.03), followed in descending order by those of shoveler (10.31 ± 0.05), Gadwall (9.92 ± 0.04), Mallard (8.91 ± 0.04), and pintail (7.66 ± 0.06). In Mallard, pintail, and …

Less Waste Corn, More Land In Soybeans, And The Switch To Genetically Modified Crops: Trends With Important Implications For Wildlife Management, Gary L. Krapu, David A. Brandt, Robert R. Cox Jr.

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

American agriculture has provided abundant high-energy foods for migratory and resident wildlife populations since the onset of modern wildlife management. Responding to anecdotal evidence that corn residues are declining in cropland, we remeasured waste corn postharvest in the Central Platte River Valley (CPRV) of Nebraska during 1997 and 1998 to compare with 1978. Post-harvest waste corn averaged 2.6% and 1.8% of yield in 1997 and 1998, respectively. After accounting for a 20% increase in yield, waste corn in 1997 and 1998 was reduced 24% and 47% from 1978. We also evaluated use of soybeans by spring-staging sandhill cranes ( Grus …

Tracking Of White-Tailed Deer Migration By Global Positioning System, Michael Nelson, L. David Mech, Paul F. Frame

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

We used global positioning system (GPS) radiocollars on female white-tailed deer (Odocoileus virginianus) to document details of onsets of migrations, rates of travel, patterns of travel, durations of migrations, and distances traveled by 8 deer in spring and 4 deer in autumn in northeastern Minnesota in 1998, 1999, and 2001. In spring, deer migrated 23–45 km during 31–356 h, deviating a maximum 1.6–4.0 km perpendicular from a straight line of travel between their seasonal ranges. They migrated a minimum of 2.1–18.6 km/day over 11–56 h during 2–14 periods of travel. Minimum travel during 1-h intervals averaged 1.5 km/h …

Is Climate Change Affecting Wolf Populations In The High Artic?, L. David Mech

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Gobal climate change may affect wolves in Canada’s High Arctic (80◦ N) acting through three trophic levels (vegetation, herbivores, and wolves). A wolf pack dependent on muskoxen and arctic hares in the Eureka area of Ellesmere Island denned and produced pups most years from at least 1986 through 1997. However, when summer snow covered vegetation in 1997 and 2000 for the first time since records were kept, halving the herbivore nutrition-replenishment period, muskox and hare numbers dropped drastically, and the area stopped supporting denning wolves through 2003. The unusual weather triggering these events was consistent with global-climate-change phenomena.

Seasonal Activity Areas Of Coyotes In The Bear River Mountains Of Utah And Idaho, Glen F. Gantz, Frederick F. Knowlton

United States Department of Agriculture Wildlife Services: Staff Publications

We studied the seasonal movement patterns and dispersal of coyotes (Canis latrans) in the Bear River Mountains of northern Utah and southern Idaho to determine whether coyotes in this montane region exhibit an altitudinal migration on a seasonal basis. We used 3 locational parameters to assess whether a seasonal altitudinal migration was evident, including overlap in seasonal activity areas, distance between harmonic mean centers of activity, and seasonal differences in mean elevations of locations. Winter and summer activity areas of every mature coyote overlapped, with mean distances between harmonic centers of seasonal activity of 1.5 km (range = 0.4–3.3 km). …

Predacides For Canid Predation Management, K. A. Fagerstone, J. J. Johnston, P. J. Savarie

United States Department of Agriculture Wildlife Services: Staff Publications

Throughout the livestock industry in the western United States, control of canid predators was considered to be of considerable importance to the livestock industry, especially to sheep producers, who sufkred high losses from coyotes and wolves. In the 19th century, the demand for predator control was communicated to Congress and the western state assemblies, with the result that predator control was provided in western states by the Fedcral Bureau of Biological Survey in cooperation with state agencies, and by trappers hired by stockmen. Steel traps and poisons were the principal methods used for predator control during the early years of …

A Conceptual Framework For Non-Kin Food Sharing: Timing And Currency Of Benefits, Jeffrey R. Stevens, Ian C. Gilby

Jeffrey Stevens Publications

Many animal species, from arthropods to apes, share food. This paper presents a new framework that categorizes nonkin food sharing according to two axes: (1) the interval between sharing and receiving the benefits of sharing, and (2) the currency units in which benefits accrue to the sharer (especially food versus nonfood). Sharers can obtain immediate benefits from increased foraging efficiency, predation avoidance, mate provisioning, or manipulative mutualism. Reciprocity, trade, status enhancement and group augmentation can delay benefits. When benefits are delayed or when food is exchanged for nonfood benefits, maintaining sharing can become more difficult because animals face discounting and …

Emergence And Survival Of Legumes Seeded Into Pastures Varying In Landscape Position, John A. Guretzky, Kenneth J. Moore, Allen D. Knapp, E. Charles Brummer

Department of Agronomy and Horticulture: Faculty Publications

Landscape position affects legume establishment in pastures. We conducted this study to (i) determine the role of emergence and survival on establishment of legumes on summit and backslope positions and (ii) examine how competition, as influenced by sward-cutting height and N fertilization, affects legume emergence and survival across these positions. We no-till drilled a mixture composed of red clover (Trifolium pratense L.), alfalfa (Medicago sativa L.), and birdsfoot trefoil (Lotus corniculatus L.) into an existing cool-season grass pasture at Rhodes, IA, in 1998 and 1999. Treatments consisted of landscape positions (summit and backslope), N fertilizer rates (0, …

Molecular Mapping And Identification Of Soybean Fatty Acid Modifier Quantitative Trait Loci, D. L. Hyten Jr., Vincent R. Pantalone, Arnold M. Saxton, Michael E. Schmidt, Carl E. Sams

Department of Agronomy and Horticulture: Faculty Publications

Altering FA content in soybean [Glycine max (L.) Merr.] oil for improved functionality is a research goal of many soybean breeders. Several of the genes that alter palmitic, stearic, oleic, linoleic, and linolenic acids are modifier genes with small effects, causing these FA traits to act as quantitative traits. The objective of this study was to identify modifier FA quantitative trait loci (QTL) in soybean. A recombinant inbred line population was created from two prominent ancestors of currently avail-able U.S. cultivars (Essex and Williams) and grown in five environments. One hundred simple se-quence repeat markers spaced throughout the genome …

Changes In Composition And Thermal Transition Temperatures Of Grain Sorghum Wax During Storage, Keum Taek Hwang, Curtis L. Weller, Susan L. Cuppett, Milford Hanna

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Grain sorghum (Sorghum bicolor) wax is composed mainly of aldehydes, alcohols, and acids. Aldehydes, comprising about one-half of the wax, are readily converted to acids in presence of air. In this study, whole sorghum wax and an aldehyde fraction from sorghum wax were subjected to oxidative conditions. Changes in the major components and thermal transition temperatures were determined using HPLC and differential scanning calorimeter (DSC), respectively. The aldehyde fraction was oxidized markedly to acids over 4 months in storage at room temperature. Acid content, in the fraction, was initially 5–7% and increased to 42–51% after 135 days in …

Identification Of Methionine-Rich Clusters That Regulate Copper-Stimulated Endocytosis Of The Human Ctr1 Copper Transporter, Yan Guo

Department of Biochemistry: Faculty Publications

Copper uptake and subsequent delivery to copper-dependent

enzymes are essential for many cellular processes.

However, the intracellular levels of this nutrient

must be controlled because of its potential toxicity. The

hCtr1 protein functions in high affinity copper uptake

at the plasma membrane of human cells. Recent studies

have shown that elevated copper stimulates the endocytosis

and degradation of the hCtr1 protein, and this

response is likely an important homeostatic mechanism

that prevents the overaccumulation of copper. The domains

of hCtr1 involved in copper-stimulated endocytosis

and degradation are unknown. In this study we examined

the importance of potential copper-binding

sequences in …

Mobilization Of Intracellular Copper Stores By The Ctr2 Vacuolar Copper Transporter, Erin M. Rees, Jaekwon Lee, Dennis J. Thiele

Department of Biochemistry: Faculty Publications

Copper plays an essential role in processes including signaling to the transcription and protein trafficking machinery, oxidative phosphorylation, iron mobilization, neuropeptide maturation, and normal development. Whereas much is known about intracellular mobilization of ions such as calcium, little information is available on how eukaryotic cells mobilize intracellular copper stores. We describe a mechanism by which the Saccharomyces cerevisiae Ctr2 protein provides bioavailable copper via mobilization of intracellular copper stores. Whereas Ctr2 exhibits structural similarity to the Ctr1 plasma membrane copper importer, microscopic and biochemical fractionation studies localize Ctr2 to the vacuole membrane. We demonstrate that Ctr2 mobilizes vacuolar copper stores …

Host–Symbiont Stability And Fast Evolutionary Rates In An Ant–Bacterium Association: Cospeciation Of Camponotus Species And Their Endosymbionts, Candidatus Blochmannia, Patrick H. Degnan, Adam B. Lazarus, Chad D. Brock, Jennifer J. Wernegreen

School of Biological Sciences: Faculty Publications

Bacterial endosymbionts are widespread across several insect orders and are involved in interactions ranging from obligate mutualism to reproductive parasitism. Candidatus Blochmannia gen. nov. (Blochmannia) is an obligate bacterial associate of Camponotus and related ant genera (Hymenoptera: Formicidae). The occurrence of Blochmannia in all Camponotus species sampled from field populations and its maternal transmission to host offspring suggest that this bacterium is engaged in a long-term, stable association with its ant hosts. However, evidence for cospeciation in this system is equivocal because previous phylogenetic studies were based on limited gene sampling, lacked statistical analysis of congruence, and have …