Digital Commons Network^™

Stat5a Promotes Adipogenesis In Nonprecursor Cells And Associates With The Glucocorticoid Receptor During Adipocyte Differentiation, Z. Elizabeth Floyd, Jacqueline M. Stephens

Faculty Publications

The differentiation of adipocytes is regulated by the activity of a variety of transcription factors, including peroxidase proliferator-activated receptor (PPAR)-γ and C/EBPα. Our current study demonstrates that ectopic expression of STAT5A, such as that of PPAR-γ and C/EBPα, promotes adipogenesis in two nonprecursor fibroblast cell lines. Using morphologic and biochemical criteria, we have demonstrated that STAT5A and the combination of STAT5A and STAT5B are sufficient to induce the expression of early and late adipogenic markers in BALB/c and NIH-3T3 cells. Yet, the ectopic expression of STAT5B alone does not induce the expression of adipocyte genes, but enhances the induction of …

Methylation-Sensitive Binding Of Transcription Factor Yy1 To An Insulator Sequence Within The Paternally Expressed Imprinted Gene, Peg3, Joomyeong Kim, Angela Kollhoff, Anne Bergmann, Lisa Stubbs

Faculty Publications

The 5�-ends of two paternally expressed mouse genes, Peg3 and Usp29, are jointly associated with a CpG island that exhibits allele-specific methylation. Sequence comparison of the regions derived from human, mouse and cow revealed the presence of two evolutionarily conserved sequence motifs including one that is repeated multiple times within the first intron of Peg3 in all three mammals. DNA mobility shift and chromatin immunoprecipitation (ChlP) assays clearly demonstrated that this motif is an in vivo binding site for the Gli-type transcription factor YY1. The YY1-binding site contains one CpG dinucleotide, and methylation of this CpG site abolishes the binding …

Wright State University College Of Engineering And Computer Science Bits And Pcs Newsletter, Volume 19, Number 4, February 2003, College Of Engineering And Computer Science, Wright State University

BITs and PCs Newsletter

An eight page newsletter created by the Wright State University College of Engineering and Computer Science that addresses the current affairs of the college.

Transcriptional Initiation Under Conditions Of Anoxia-Induced Quiescence In Mitochondria From Artemia Franciscana Embryos, Brian D. Eads, Steven C. Hand

Faculty Publications

In response to anoxia, embryos of the brine shrimp Artemia franciscana are able coordinately to downregulate metabolism to levels low enough to permit survival for several years at room temperature. In addition to dramatic decreases in free ATP levels and heat production, intracellular pH drops from 7.8 to 6.3 overnight. Use of isolated mitochondria to study transcriptional responses to anoxia offers several advantages: (1) the localized nature of transcript initiation, processing and degradation, all of which may be followed in organello; (2) the relatively simple cis- and trans-machinery involved and (3) the ability to provide relevant physiological treatments in vitro. …

Localization And Characterization Of Phenamil-Sensitive Na+ Influx In Isolated Rainbow Trout Gill Epithelial Cells, Scott D. Reid, G. S. Hawkings, F. Galvez, G. G. Goss

Faculty Publications

Percoll density-gradient separation, combined with peanut lectin agglutinin (PNA) binding and magnetic bead separation, was used to separate dispersed fish gill cells into sub-populations. Functional characterization of each of the sub-populations was performed to determine which displayed acid-activated phenamil- and bafilomycin-sensitive Na+ uptake. Analysis of the mechanism(s) of 22Na+ influx was performed in control and acid-activated (addition of 10 mmoll-1 proprionic acid) cells using a variety of Na+ transport inhibitors (ouabain, phenamil, HOE-694 and bumetanide) and a V-type ATPase inhibitor (bafilomycin). We found that cells migrating to a 1.03-1.05 g ml-1 Percoll interface [pavement cells (PVCs)] possessed the lowest rates …

A Meta-Analysis Of Genome-Wide Linkage Scans For Hypertension: The National Heart, Lung And Blood Institute Family Blood Pressure Program, Michael A. Province, Sharon L.R. Kardia, Koustubh Ranade, Dabeeru C. Rao, Bonnie A. Thiel, Richard S. Cooper, Neil Risch, Stephen T. Turner, David R. Cox, Steven C. Hunt, Alan B. Weder, Eric Boerwinkle

Faculty Publications

Background: Four multicenter Networks (GenNet, GENOA, HyperGEN, SAPPHIRe) form the National Heart, Lung and Blood Institute Family Blood Pressure Program (FBPP), to search for hypertension/blood pressure (BP) genes. The networks used different family designs and targeted multiple ethnic groups, using standardized protocols and definitions. Linkage genome scans were done on samples within each network (N = 6245 relatives). Methods: The evidence was synthesized using meta-analysis. Results: Combining ethnic groups, no region reached LOD >2, but several small peaks were identified, including chromosome 2p where two other recent reports find hypertension linkage. Conclusions: No regions show uniformly large effects on BP/hypertension …

Race, Genes, And Health - New Wine In Old Bottles?, Richard S. Cooper

Faculty Publications

No abstract provided.

Effect Of Integrating A Sportfishing Curriculum Into A Camp Program On The Knowledge, Awareness, And Attitudes Of Participating Youth, Keith Koupal, Marianne Krasny

Journal of Extension

We evaluated the effect of incorporating a sportfishing and environmental curriculum into a short-term summer camp program on participating youths' knowledge of fishing and biology/ecology, awareness of ethical behavior, and attitudes concerning fishing and saving the environment. Using a pre-/post-survey of camp participants, we determined that the program was successful in developing youth knowledge related to fishing skills and biology/ecology, but did not affect ethical behavior awareness or attitudes of participants. Although longer-term programs may be needed to affect youth attitudes, camp programs offer the opportunity to increase knowledge among a large number of youth with a minimal investment in …

Energetic Adaptations Along A Broad Latitudinal Gradient: Implications For Widely Distributed Assemblages, James E. Garvey, Dennis R. Devries, Russell A. Wright, Jeffrey G. Miner

Publications

Most community-based models in ecology assume that all individuals within a species respond similarly to environmental conditions and thereby exert identical effects as consumers or prey. Rather, individuals differ among systems, with important implications for population demographics and community interactions. For widely distributed assemblages made up of poikilotherms with high first-year mortality, species-specific differences in growth reaction norms as affected by both temperature and genotype will influence biotic interactions. For a broadly distributed fish assemblage, first-year growth does not vary with latitude for a planktivorous prey species, but declines with increasing latitude for a terminal piscivore. Size-based competitive interactions between …

Rhamnolipid Surfactant Production Affects Biofilm Architecture In Pseudomonas Aeruginosa Pao1, Mary E. Davey, Nicky C. Caiazza, George A. O'Toole

Dartmouth Scholarship

In response to certain environmental signals, bacteria will differentiate from an independent free-living mode of growth and take up an interdependent surface-attached existence. These surface-attached microbial communities are known as biofilms. In flowing systems where nutrients are available, biofilms can develop into elaborate three-dimensional structures. The development of biofilm architecture, particularly the spatial arrangement of colonies within the matrix and the open areas surrounding the colonies, is thought to be fundamental to the function of these complex communities. Here we report a new role for rhamnolipid surfactants produced by the opportunistic pathogen Pseudomonas aeruginosa in the maintenance of biofilm architecture. …

Uterine Neoplasm Resembling An Ovarian Sex Cord Tumor, Sarah Kuruvila, Dushyanti Samarasinghe, Sosamma Thomas

Saudi Medical Journal

Uterine neoplasm resembling an ovarian sex cord tumor is a rare neoplasm. Patients are generally of reproductive age, although a few may be perimenopausal or post menopausal. This neoplasm produces abnormal vaginal bleeding and uterine enlargement, suggesting leiomyoma or polyps. Based on the histology and clinical outcome, this tumor can be classified into 2 groups, namely one with focal areas resembling sex cord elements with a tendency to recur or metastasize and another with exclusive sex cord elements which runs a benign course. Immunohistochemical stains of the sex cord elements may show positively for vimentin, cytokeratin, actin and desmin in …

Oncolog, Volume 48, Number 02, February 2003, Sunni Hosemann, Dawn Chalaire, Michael J. Miller Md, Facs

OncoLog MD Anderson's Report to Physicians (All issues) Archives

• Tissue Engineering: Several Avenues of Research Lead Toward a Single Goal
• Researchers Design a Unique Stereotactic Radiotherapy and Radiosurgery System to Treat Patients with Paraspinal Metastases
• House Call: Women and Lung Cancer: News You Can Use
• DiaLog: Applying Tissue Engineering Techniques in the Clinic and the Laboratory, by Michael J. Miller, MD, FACS, Professor, Department of Plastic Surgery

Nebline, February 2003

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Access Extension’s Extensive Resources On the Information Highway
An Inside Look at lancaster.unl.edu
2003 All-America Rose Selections
Garden Guide
Hints for Starting Transplants
Azalea Plant Care
Check for Vole Damage
Hunting Workshop: Spring Turkey
Protect Your Well Water
Natural Insecticides Aren.t Always So Safe
Chemigation Training Feb. 18
Computerized Financial Record Keeping Workshops
Unwanted Pesticide Collection March 17
Workshop Dates and Locations
Latest U.S. Drought Monitor Map
Selling Horticulture Produce
Selecting and Using Hardwood Firewood
Cleaning the Kitchen Cupboard: Can This Food Be Saved?
Healthy Eating: Enjoy Nebraska Foods
Making the Five Food Groups “Come Alive” for Pathways Families
Web …

Genetic Variation In The Midcontinental Population Of Sandhill Cranes, Grus Canadensis, Jessica L. Petersen, Richard Bischof, Gary L. Krapu, Allen L. Szalanski

Nebraska Game and Parks Commission: Staff Research Publications

Three subspecies of sandhill crane (Grus canadensis) are recognized in the Midcontinental population, the lesser (Grus c. canadensis), Canadian (G. c. rowani), and greater (G. c. tabida). Blood samples collected on the population's primary spring staging area in Nebraska, U.S.A., were used to resolve the genetic relationship among these subspecies. Phylogenetic analysis of 27 G. canadensis, by DNA sequencing of a 675 bp region of the mtDNA, supports the subspecies designations of G. c. canadensisand G. c. tabida. G. c. rowani individuals were intermediate with each of the other two …

Water Current, Volume 35, No. 1, February 2003

Water Current Newsletter

Plans Taking Shape for Summer Tour; Geologists to Meet In Lincoln
Meet the Faculty
New Analytic Equipment Increases Water Sciences Lab Capabilities
Effects of Heavy Sedimentation on Rainwater Basin Wetland Hydrology
Students Get Their Feet Wet at Audubon Spring Creek Prairie
Water News Briefs
Free Water and Natural Resources Lectures Continue at UNL

Wright State University Softball Media Guide 2003, Wright State University Athletics

Athletics Publications

A media guide for Wright State University's 2003 softball team. This guide includes photographs, player stats, game results, and records.

The Child Citizenship Act: Too Little, Too Late For Tuan Nguyen, Ashley Moore

William & Mary Journal of Race, Gender, and Social Justice

No abstract provided.

Location And Life: How Stenberg V. Carhart Undercut Roe V. Wade, Richard Stith

William & Mary Journal of Race, Gender, and Social Justice

No abstract provided.

Tumbling Towers As Turning Points: Will 9/11 Usher In A New Civil Rights Era For Gay Men And Lesbians In The United States?, Susan J. Becker

William & Mary Journal of Race, Gender, and Social Justice

No abstract provided.

The Impact Of Future Orientation On The Avoidance Of Teen Pregnancy, Cheryl L. Somers, Traci A. Gizzi, Stephanie A. Johnson

Michigan Journal of Counseling: Research, Theory and Practice

This study explores the relationship between future orientation and teens’ attitudes about pregnancy and avoidance behaviors. Participants were 91 adolescents, ages 15-19, surveyed using measures developed for this study that examined future orientation, attitudes to avoiding pregnancy, child-rearing responsibility, and perceptions about teen parenting. The results suggested that finishing high school was important in predicting a desire to avoid pregnancy. The perception that a teen pregnancy would make it difficult to achieve post-high school educational goals, specifically a college education, was also found to be important. Implications for school counselors and mental health professionals are discussed. Issues specific to non …

Municipal Storm Water Permitting In California, John H. Minan

San Diego Law Review

This Article discusses the regulation of discharges of waste in storm water from municipal separate storm sewer systems and the consequent legal challenges. The author begins by examining the current structure of federal and state regulation of storm water pollution, focusing his analysis on California. Professor Minan then addresses the storm water pollution problem and analyzes the San Diego MS4 permit as an example of the challenges that municipalities face in the permitting process. The author explores the various legal concerns that stem from both state and federal regulation of this area of pollution and provides a framework for understanding …

Monthly Accomplishments For Turnbull National Refuge, February 2003, Turnbull National Wildlife Refuge

Turnbull National Wildlife Refuge Reports

A monthly report of accomplishments at Turnbull National Wildlife Refuge written by Project Leader Nancy Curry.

Biol 104n.00: Diversity Of Life Laboratory, Unknown

University of Montana Course Syllabi, 1990-2010

No abstract provided.

Perspective, Vol. 14 No. 2, Winter 2003, Eastern Washington University. Division Of University Relations.

Alumni Publications

This issue contains articles about the Department of Urban and Regional Planning, alumnus Frank Armijo and the Hispanic Academic Achievers Program (HAAP), the Women's Studies Center, which held a 25th anniversary celebration in November 2002, and McNair Scholars at Eastern.

Psc 150e.01: Introduction To Political Theory, Ramona Grey

University of Montana Course Syllabi

No abstract provided.

Multiple Roles For Hedgehog Signaling In Zebrafish Pituitary Development, Jennifer L. Sbrogna, Michael J.F. Barresi, Rolf O. Karlstrom

Biological Sciences: Faculty Publications

The endocrine-secreting lobe of the pituitary gland, or adenohypophysis, forms from cells at the anterior margin of the neural plate through inductive interactions involving secreted morphogens of the Hedgehog (Hh), fibroblast growth factor (FGF), and bone morphogenetic protein (BMP) families. To better understand when and where Hh signaling influences pituitary development, we have analyzed the effects of blocking Hh signaling both pharmacologically (cyclopamine treatments) and genetically (zebrafish Hh pathway mutants). While current models state that Shh signaling from the oral ectoderm patterns the pituitary after placode induction, our data suggest that Shh plays a direct early role in both pituitary …

Integrating Service-Learning Into Watershed Management Programs: Opportunities And Challenges, Tamim Younos, Raymond De Leon, Christine Lewicki

Special Topics, General

The objective of this article is to open a dialogue on integrating service-learning into community based watershed management programs and to discuss opportunities and challenges that a service-learning program presents to universities and communities. The article presents the concept and definition of service-learning, and arguments concerning why institutions of higher education and university faculty and students should be involved with community based watershed management programs.

Paralyzing Discord: Workplace Safety, Paternalism, And The Accommodation Of Biological Variance In The Americans With Disabilities Act, Alexandra G. White

Louisiana Law Review

No abstract provided.

Efficient Incorporation Of Coa, Nad And Fad Into Rna By In Vitro Transcription, Faqing Huang

Faculty Publications

Protein enzymes frequently recruit small molecule coenzymes to perform a variety of biochemical reactions. While the catalytic activities of RNA have been expanding rapidly, a similar strategy for RNA to utilize coenzymes and to increase its functional capabilities has yet to be demonstrated. A general in vitro transcription procedure has been developed to efficiently prepare RNA with coenzymes CoA, NAD and FAD covalently attached to the 5' end. These adenosine-containing coenzymes initiate transcription under the T7 class II promoter by T7 RNA polymerase. In addition to the three coenzymes, other adenosine-containing molecules may be incorporated into the first nucleotide position …

When Life Is An Injury: An Economic Approach To Wrongful Life Lawsuits, Thomas A. Burns

Duke Law Journal

No abstract provided.