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2002

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Phylogenetic And Phenotypic Analysis Of Hiv Type 1 Env Gp120 In Cases Of Subtype C Mother-To-Child Transmission, Hong Zhang, Guillermo Ortí, Qiujiang Du, Jun He, Chipepo Kankasa, Ganapati Bhat, Charles Wood Jan 2002

Phylogenetic And Phenotypic Analysis Of Hiv Type 1 Env Gp120 In Cases Of Subtype C Mother-To-Child Transmission, Hong Zhang, Guillermo Ortí, Qiujiang Du, Jun He, Chipepo Kankasa, Ganapati Bhat, Charles Wood

Nebraska Center for Virology: Faculty Publications

Human immunodeficiency virus type 1 (HIV-1) subtype C constitutes a majority of the newly transmitted cases of HIV-1 in many developing countries. There is growing evidence suggesting that subtype C viruses may display characteristics that make them distinct from subtype B and other subtypes, and such differences may affect its transmission and pathogenesis in infected individuals. In this study, HIV-1 sequences from the C2–V4 region of the envelope gene were analyzed from four infected mother–infant pairs (MIPs) after perinatal transmission in Zambia. We found that all viral isolates from four Zambian MIPs were of the subtype C HIV-1 subgroup. All …

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Small Glutamine-Rich Protein/Viral Protein U–Binding Protein Is A Novel Cochaperone That Affects Heat Shock Protein 70 Activity, Peter C. Angeletti, Doriann Walker, Antonito T. Panganiban Jan 2002

Small Glutamine-Rich Protein/Viral Protein U–Binding Protein Is A Novel Cochaperone That Affects Heat Shock Protein 70 Activity, Peter C. Angeletti, Doriann Walker, Antonito T. Panganiban

Nebraska Center for Virology: Faculty Publications

Molecular chaperone complexes containing heat shock protein (Hsp) 70 and Hsp90 are regulated by cochaperones, including a subclass of regulators, such as Hsp70 interacting protein (Hip), C-terminus of Hsp70 interacting protein (CHIP), and Hsp70-Hsp90 organizing factor (Hop), that contain tetratricopeptide repeats (TPRs), where Hsp70 refers to Hsp70 and its nearly identical constitutive counterpart, Hsc70, together. These proteins interact with the Hsp70 to regulate adenosine triphosphatase (ATPase) and folding activities or to generate the chaperone complex. Here we provide evidence that small glutamine-rich protein/viral protein U–binding protein (SGT/UBP) is a cochaperone that negatively regulates Hsp70. By “Far-Western” and pull-down assays, SGT/UBP …

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Responses Of Dabbling Ducks To Wetland Conditions In The Prairie Pothole Region, Jane Austin Jan 2002

Responses Of Dabbling Ducks To Wetland Conditions In The Prairie Pothole Region, Jane Austin

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

The relationships between wetland water conditions and breeding numbers of Mallard (Anas platyrhynchos), Northern Pintail (A. acuta), Blue-winged Teal (A. discors), and Northern Shoveler (A. clypeata) during May of 1992-1995, were examined on twelve study areas in the eastern Prairie Pothole Region. Data were collected on water levels (by wetland class [temporary, seasonal, semipermanent]), pond density (density of wet basins), and numbers of indicated pairs for each species from weekly roadside transect surveys. Comparison of models relating duck numbers to wetlands using Akaike’s Information Criterion indicated that measures of water condition generally …

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Diet Of Shovelnose Sturgeon Downstream From Gavins Point Dam: Final Report, Charles R. Berry Jr. Jan 2002

Diet Of Shovelnose Sturgeon Downstream From Gavins Point Dam: Final Report, Charles R. Berry Jr.

United States Army Corps of Engineers, Omaha District

The diet of shovelnose sturgeon (Scaphirhynchus platorhynchus) in the Missouri River downstream from Gavins Point Dam was characterized in 1993 (N=100 fish) and 1994 (N=103 fish) for comparison with similar data collected in 1971-72 (N=130 fish) by Modde and Schmulbach (1977, hereafter Modde). The primary difference among years in the South Dakota studies was river discharge, which was low in 1993 (9-22 kcfs; kcfs=thousand cubic feet per second), moderate in 1994 (18-31 kcfs), and high in 1971-72 (26-49 kcfs). We compared sturgeon diet weight and the weight of benthic and drifting invertebrates among years, among monthly discharges in …

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Does Increasing Daylength Control Seasonal Changes In Clutch Sizes Of Northern Pintails (Ana Acuta)?, Gary Krapu, Glen Sergeant, Alison Perkins Jan 2002

Does Increasing Daylength Control Seasonal Changes In Clutch Sizes Of Northern Pintails (Ana Acuta)?, Gary Krapu, Glen Sergeant, Alison Perkins

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

We evaluated spatiotemporal variation in clutch sizes of Northern Pintails (pintails; Anas acuta) nesting in California (1985 to 1996), North Dakota (1982 to 1985), Saskatchewan (1982 to 1985) and Alaska (1991 to 1993) to determine whether seasonal declines in clutch size varied in ways that were consistent with a controlling influence of increasing day length. Pintails began nesting in mid-March in California, mid-April in North Dakota and Saskatchewan, and mid-May in Alaska. Observed durations of nesting were 70 ± 2.6 days (SE) in California, 60 ± 6.3 days in North Dakota, 66 ± 1.3 days in Saskatchewan, and 42 …

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Effects Of Management Practices On Grassland Birds: Dickcissel, Jill A. Dechant, Marriah L. Sondreal, Douglas H. Johnson, Lawrence D. Igl, Christopher M. Goldade, Amy L. Zimmerman, Betty R. Euliss Jan 2002

Effects Of Management Practices On Grassland Birds: Dickcissel, Jill A. Dechant, Marriah L. Sondreal, Douglas H. Johnson, Lawrence D. Igl, Christopher M. Goldade, Amy L. Zimmerman, Betty R. Euliss

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Dickcissel (Spiza americana):
Breeding range
Suitable habitat
Area requirements
Brown-headed Cowbird brood parasitism
Breeding-season phenology and site fidelity
Species’ response to management
Management Recommendations
Habitat Characteristics

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Effects Of Management Practices On Grassland Birds: Western Meadowlark, Jill A. Dechant, Marriah L. Sondreal, Douglas H. Johnson, Lawrence D. Igl, Christopher M. Goldade, Amy L. Zimmerman, Betty R. Euliss Jan 2002

Effects Of Management Practices On Grassland Birds: Western Meadowlark, Jill A. Dechant, Marriah L. Sondreal, Douglas H. Johnson, Lawrence D. Igl, Christopher M. Goldade, Amy L. Zimmerman, Betty R. Euliss

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Western Meadowlark (Sturnella neglecta):
Breeding range
Suitable habitat
Area requirements
Brown-headed Cowbird brood parasitism
Breeding-season phenology and site fidelity
Species’ response to management
Management Recommendations
Habitat Characteristics

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Effects Of Management Practices On Grassland Birds: Vesper Sparrow, Jill A. Dechant, Meghan F. Dinkins, Douglas H. Johnson, Lawrence D. Igl, Christopher M. Goldade, Betty R. Euliss Jan 2002

Effects Of Management Practices On Grassland Birds: Vesper Sparrow, Jill A. Dechant, Meghan F. Dinkins, Douglas H. Johnson, Lawrence D. Igl, Christopher M. Goldade, Betty R. Euliss

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Upland Sandpiper (Pooecetes gramineus):
Breeding range
Suitable habitat
Area requirements
Brown-headed Cowbird brood parasitism
Breeding-season phenology and site fidelity
Species’ response to management
Management Recommendations
Habitat Characteristics

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Effects Of Management Practices On Grassland Birds: Upland Sandpiper, Jill A. Dechant, Meghan F. Dinkins, Douglas H. Johnson, Lawrence D. Igl, Christopher M. Goldade, Barry D. Parkin, Betty R. Euliss Jan 2002

Effects Of Management Practices On Grassland Birds: Upland Sandpiper, Jill A. Dechant, Meghan F. Dinkins, Douglas H. Johnson, Lawrence D. Igl, Christopher M. Goldade, Barry D. Parkin, Betty R. Euliss

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Upland Sandpiper (Bartramia longicauda):
Breeding range
Suitable habitat
Area requirements
Brown-headed Cowbird brood parasitism
Breeding-season phenology and site fidelity
Species’ response to management
Management Recommendations
Habitat Characteristics

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Effects Of Management Practices On Grassland Birds: Nelson’S Sharp-Tailed Sparrow, Jill A. Dechant, Marriah L. Sondreal, Douglas H. Johnson, Lawrence D. Igl, Christopher M. Goldade, Paul A. Rabie, Betty R. Euliss Jan 2002

Effects Of Management Practices On Grassland Birds: Nelson’S Sharp-Tailed Sparrow, Jill A. Dechant, Marriah L. Sondreal, Douglas H. Johnson, Lawrence D. Igl, Christopher M. Goldade, Paul A. Rabie, Betty R. Euliss

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Nelson’s Sharp-Tailed Sparrow ( Ammodramus nelsoni nelsoni):
Breeding range
Suitable habitat
Area requirements
Brown-headed Cowbird brood parasitism
Breeding-season phenology and site fidelity
Species’ response to management
Management Recommendations
Habitat Characteristics

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Immobilization Of Swift Foxes With Ketamine Hydrochloride-Xylazine Hydrochloride, Rebecca L. Telesco, Marsha A. Sovada Jan 2002

Immobilization Of Swift Foxes With Ketamine Hydrochloride-Xylazine Hydrochloride, Rebecca L. Telesco, Marsha A. Sovada

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

There is an increasing need to develop field immobilization techniques that allow researchers to handle safely swift foxes (Vulpes velox) with minimal risk of stress or injury. We immobilized captive swift foxes to determine the safety and effectiveness of ketamine hydrochloride and xylazine hydrochloride at different dosages. We attempted to determine appropriate dosages to immobilize swift foxes for an adequate field-handling period based on three anesthesia intervals (induction period, immobilization period, and recovery period) and physiologic responses (rectal temperature, respiration rate, and heart rate). Between October 1998–July 1999, we conducted four trials, evaluating three different dosage ratios of …

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Monitoring Black-Tailed Prairie Dog Colonies With High-Resolution Satellite Imagery, John G. Sidle, Douglas H. Johnson, Betty R. Euliss, Malrcus Tooze Jan 2002

Monitoring Black-Tailed Prairie Dog Colonies With High-Resolution Satellite Imagery, John G. Sidle, Douglas H. Johnson, Betty R. Euliss, Malrcus Tooze

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

The United States Fish and Wildlife Service has determined that the black-tailed prairie dog (Cynomys ludovicianus) warrants listing as a threatened species under the Endangered Species Act. Central to any conservation planning for the black-tailed prairie dog is an appropriate detection and monitoring technique. Because coarse-resolution satellite imagery is not adequate to detect black-tailed prairie dog colonies, we examined the usefulness of recently available high-resolution (1-m) satellite imagery. In 6 purchased scenes of national grasslands, we were easily able to visually detect small and large colonies without using image-processing algorithms. The Ikonos (Space Imaging™) satellite imagery was as …

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Effects Of Management Practices On Grassland Birds: Sedge Wren, Jill A. Dechant, Marriah L. Sondreal, Douglas H. Johnson, Lawrence D. Igl, Christopher M. Goldade, Barry D. Parkin, Betty R. Euliss Jan 2002

Effects Of Management Practices On Grassland Birds: Sedge Wren, Jill A. Dechant, Marriah L. Sondreal, Douglas H. Johnson, Lawrence D. Igl, Christopher M. Goldade, Barry D. Parkin, Betty R. Euliss

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Sedge Wren (Cistothorus platensis):
Breeding range
Suitable habitat
Area requirements
Brown-headed Cowbird brood parasitism
Breeding-season phenology and site fidelity
Species’ response to management
Management Recommendations
Habitat Characteristics

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Effects Of Management Practices On Grassland Birds: Northern Harrier, Jill A. Dechant, Marriah L. Sondreal, Douglas H. Johnson, Lawrence D. Igl, Christopher M. Goldade, Melvin P. Nenneman, Betty R. Euliss Jan 2002

Effects Of Management Practices On Grassland Birds: Northern Harrier, Jill A. Dechant, Marriah L. Sondreal, Douglas H. Johnson, Lawrence D. Igl, Christopher M. Goldade, Melvin P. Nenneman, Betty R. Euliss

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Northern Harrier (Circus cyaneus):
Breeding range
Suitable habitat
Prey habitat
Area requirements
Brown-headed Cowbird brood parasitism
Breeding-season phenology and site fidelity
Species’ response to management
Management Recommendations
Habitat Characteristics

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Effects Of Management Practices On Grassland Birds: Mountain Plover, Jill A. Dechant, Marriah L. Sondreal, Douglas H. Johnson, Lawrence D. Igl, Christopher M. Goldade, Melvin P. Nenneman, Betty R. Euliss Jan 2002

Effects Of Management Practices On Grassland Birds: Mountain Plover, Jill A. Dechant, Marriah L. Sondreal, Douglas H. Johnson, Lawrence D. Igl, Christopher M. Goldade, Melvin P. Nenneman, Betty R. Euliss

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Mountain Plover (Charadrius montanus):
Breeding range
Suitable habitat
Area requirements
Brown-headed Cowbird brood parasitism
Breeding-season phenology and site fidelity
Species’ response to management
Management Recommendations
Habitat Characteristics

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Effects Of Management Practices On Grassland Birds: Long-Billed Curlew, Jill A. Dechant, Marriah L. Sondreal, Douglas H. Johnson, Lawrence D. Igl, Christopher M. Goldade, Paul A. Rabie, Betty R. Euliss Jan 2002

Effects Of Management Practices On Grassland Birds: Long-Billed Curlew, Jill A. Dechant, Marriah L. Sondreal, Douglas H. Johnson, Lawrence D. Igl, Christopher M. Goldade, Paul A. Rabie, Betty R. Euliss

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Long-Billed Curlew (Numenius americanus):
Breeding range
Suitable habitat
Area requirements
Brown-headed Cowbird brood parasitism
Breeding-season phenology and site fidelity
Species’ response to management
Management Recommendations
Habitat Characteristics

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Effects Of Management Practices On Grassland Birds: Loggerhead Shrike, Jill A. Dechant, Marriah L. Sondreal, Douglas H. Johnson, Lawrence D. Igl, Christopher M. Goldade, Melvin P. Nenneman, Amy L. Zimmerman, Betty R. Euliss Jan 2002

Effects Of Management Practices On Grassland Birds: Loggerhead Shrike, Jill A. Dechant, Marriah L. Sondreal, Douglas H. Johnson, Lawrence D. Igl, Christopher M. Goldade, Melvin P. Nenneman, Amy L. Zimmerman, Betty R. Euliss

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Loggerhead Shrike (Lanius ludovicianus):
Breeding range
Suitable habitat
Area requirements
Prey habitat
Brown-headed Cowbird brood parasitism
Breeding-season phenology and site fidelity
Species’ response to management
Management Recommendations
Habitat Characteristics

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Effects Of Management Practices On Grassland Birds: Lark Sparrow, Jill A. Dechant, Marriah L. Sondreal, Douglas H. Johnson, Lawrence D. Igl, Christopher M. Goldade, Barry D. Parkin, Betty R. Euliss Jan 2002

Effects Of Management Practices On Grassland Birds: Lark Sparrow, Jill A. Dechant, Marriah L. Sondreal, Douglas H. Johnson, Lawrence D. Igl, Christopher M. Goldade, Barry D. Parkin, Betty R. Euliss

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Lark Sparrow (Chondestes grammacus):
Breeding range
Suitable habitat
Area requirements
Brown-headed Cowbird brood parasitism
Breeding-season phenology and site fidelity
Species’ response to management
Management Recommendations
Habitat Characteristics

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Effects Of Management Practices On Grassland Birds: Grasshopper Sparrow, Jill A. Dechant, Marriah L. Sondreal, Douglas H. Johnson, Lawrence D. Igl, Christopher M. Goldade, Melvin P. Nenneman, Betty R. Euliss Jan 2002

Effects Of Management Practices On Grassland Birds: Grasshopper Sparrow, Jill A. Dechant, Marriah L. Sondreal, Douglas H. Johnson, Lawrence D. Igl, Christopher M. Goldade, Melvin P. Nenneman, Betty R. Euliss

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Grasshopper Sparrow (Ammodramus savannarum):
Breeding range
Suitable habitat
Area requirements
Brown-headed Cowbird brood parasitism
Breeding-season phenology and site fidelity
Species’ response to management
Management Recommendations
Habitat Characteristics

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Genomewide Identification Of Proteins Secreted By The Hrp Type Iii Protein Secretion System Of Pseudomonas Syringae Pv. Tomato Dc3000, Tanja Petnicki-Ocwieja, David J. Schneider, Vincent C. Tam, Scott T. Chancey, Libo Shan, Yashitola Jamir, Lisa M. Schechter, Misty Janes, C. Robin Buell, Xiaoyan Tang, Alan Collmer, James R. Alfano Jan 2002

Genomewide Identification Of Proteins Secreted By The Hrp Type Iii Protein Secretion System Of Pseudomonas Syringae Pv. Tomato Dc3000, Tanja Petnicki-Ocwieja, David J. Schneider, Vincent C. Tam, Scott T. Chancey, Libo Shan, Yashitola Jamir, Lisa M. Schechter, Misty Janes, C. Robin Buell, Xiaoyan Tang, Alan Collmer, James R. Alfano

Department of Plant Pathology: Faculty Publications

The ability of Pseudomonas syringae pv. tomato DC3000 to be pathogenic on plants depends on the Hrp (hypersensitive response and pathogenicity) type III protein secretion system and the effector proteins it translocates into plant cells. Through iterative application of experimental and computational techniques, the DC3000 effector inventory has been substantially enlarged. Five homologs of known avirulence (Avr) proteins and five effector candidates, encoded by genes with putative Hrp promoters and signatures of horizontal acquisition, were demonstrated to be secreted in culture and/or translocated into Arabidopsis in a Hrp-dependent manner. These 10 Hrp-dependent outer proteins (Hops) were designated HopPtoC (Avr- PpiC2 …

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The P23 Protein Of Citrus Tristeza Virus Controls Asymmetrical Rna Accumulation, Satyanarayana Tatineni, Siddarame Gowda, Maria A. Ayllon, Maria R. Albiach-Marti, Shailaja Rabindran, William O. Dawson Jan 2002

The P23 Protein Of Citrus Tristeza Virus Controls Asymmetrical Rna Accumulation, Satyanarayana Tatineni, Siddarame Gowda, Maria A. Ayllon, Maria R. Albiach-Marti, Shailaja Rabindran, William O. Dawson

Department of Plant Pathology: Faculty Publications

Citrus tristeza virus (CTV), a member of the Closteroviridae, has a 19.3-kb positive-stranded RNA genome that is organized into 12 open reading frames (ORFs) with the 10 3' genes expressed via a nested set of nine or ten 3'-coterminal subgenomic mRNAs (sgRNAs). Relatively large amounts of negative-stranded RNAs complementary to both genomic and sgRNAs accumulate in infected cells. As is characteristic of RNA viruses, wild-type CTV produced more positive than negative strands, with the plus-to-minus ratios of genomic and sgRNAs estimated at 10 to 20:1 and 40 to 50:1, respectively. However, a mutant with all of the 3' genes …

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Discounting And Reciprocity In An Iterated Prisoner’S Dilemma, David W. Stephens, Colleen M. Mclinn, Jeffrey R. Stevens Jan 2002

Discounting And Reciprocity In An Iterated Prisoner’S Dilemma, David W. Stephens, Colleen M. Mclinn, Jeffrey R. Stevens

Jeffrey Stevens Publications

The Iterated Prisoner’s Dilemma (IPD) is a central paradigm in the study of animal cooperation. According to the IPD framework, repeated play (repetition) and reciprocity combine to maintain a cooperative equilibrium. However, experimental studies with animals suggest that cooperative behavior in IPDs is unstable, and some have suggested that strong preferences for immediate benefits (that is, temporal discounting) might explain the fragility of cooperative equilibria. We studied the effects of discounting and strategic reciprocity on cooperation in captive blue jays. Our results demonstrate an interaction between discounting and reciprocity. Blue jays show high stable levels of cooperation in treatments with …

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Influence Of Protein Tyrosine Kinases On Cell Volume Change-Induced Taurine Release, Herminia Pasantes-Morales, Rodrigo Franco Jan 2002

Influence Of Protein Tyrosine Kinases On Cell Volume Change-Induced Taurine Release, Herminia Pasantes-Morales, Rodrigo Franco

School of Veterinary and Biomedical Sciences: Faculty Publications

Taurine efflux occurs in association with cell swelling in both hyposmotic and isosmotic conditions and during cell shrinkage in apoptotic death. Release occurs through a leak pathway, is largely Ca2+-independent and is sensitive to Cl– channel blockers. Taurine efflux elicited by hyposmolarity is reduced or suppressed by tyrosine kinase blockers and increased by tyrosine phosphatase inhibitors. The specific kinases involved are still unknown and may be different in the various cell types. Non-receptor and scr-related protein kinases have been identified in some cells as elements that directly phosphorylate the taurine efflux pathway. Possible tyrosine kinase targets are …

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Methods And Compositions For Protection Against Bovine Viral Diseases, Subramaniam Srikumaran Jan 2002

Methods And Compositions For Protection Against Bovine Viral Diseases, Subramaniam Srikumaran

School of Veterinary and Biomedical Sciences: Faculty Publications

The present invention relates to methods and compositions for eliciting an immune response against bovine viral epitopes. The methods comprise combining at least one heat shock protein with at least one bovine viral epitope to form a purified epitope/heat shock protein complex and administration of an immune system stimulating amount of the purified epitope/heat shock protein complex. The compositions comprise, a purified epitope/heat shock protein complex comprising at least one bovine viral epitope complexed with at least one heat shock protein, and a pharmaceutically acceptable carrier, diluent or excipient.

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Cellular Tropisms And Co-Receptor Usage Of Hiv-1 Isolates From Vertically Infected Children With Neurological Abnormalities And Rapid Disease Progression, Micheline Mccarthy, Jun He, Denise Auger, Rebeca Geffin, Cecilia Hutto, Charles Wood, Gwendolyn Scott Jan 2002

Cellular Tropisms And Co-Receptor Usage Of Hiv-1 Isolates From Vertically Infected Children With Neurological Abnormalities And Rapid Disease Progression, Micheline Mccarthy, Jun He, Denise Auger, Rebeca Geffin, Cecilia Hutto, Charles Wood, Gwendolyn Scott

Nebraska Center for Virology: Faculty Publications

The longitudinal evolution of HIV-1 phenotypes was studied in a cohort of six vertically infected children with early onset and rapid progression of clinical disease. Among 30 viral isolates obtained from peripheral blood, tropisms for both human blood-derived cells (macrophages, T-lymphocytes), and for human neural (brain-derived) cells (microglia, astrocytes) were determined, as was chemokine co-receptor usage. All children harbored from birth macrophage-tropic isolates using the CCR5 co-receptor. Two children later developed T-cell tropic isolates with CXCR4 and CCR3 usage. While all six patients developed neurological abnormalities, only three produced neural cell tropic isolates, which used CCR5. However, early and persistent …

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Unifying Lectures, Recitations & Laboratories As A Part Of The Humanized Physics Project, Vicki L. Plano Clark, Robert G. Fuller Jan 2002

Unifying Lectures, Recitations & Laboratories As A Part Of The Humanized Physics Project, Vicki L. Plano Clark, Robert G. Fuller

Robert G. Fuller Publications

As a part of our work in the Humanized Physics Project, we have made the content of the physics laboratories the keystone of our physics content coverage. After having selected an appropriate human application of physics for the laboratory each week, we have shaped the lecture, recitation and homework assignments to support the laboratory. Our poster will provide examples from both semesters of our general physics course.

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Segregation Of Spermatozoa Within Sperm Storage Tubules Of Fowl And Turkey Hens, L. M. King, J. P. Brillard, M. R. Bakst, A. M. Donoghue Jan 2002

Segregation Of Spermatozoa Within Sperm Storage Tubules Of Fowl And Turkey Hens, L. M. King, J. P. Brillard, M. R. Bakst, A. M. Donoghue

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In avian species, spermatozoa reside in the oviduct for prolonged periods in specialized structures known as sperm storage tubules, but little is known about the relative distribution of spermatozoa in these tubules after successive inseminations by different males. The staining efficacies of various fluorescent dyes for fowl and turkey spermatozoa were evaluated to investigate one proposed mechanism of sperm competition. Hens were then inseminated at different intervals with stained and unstained spermatozoa to observe the spatial distribution of spermatozoa within the storage tubules. Several novel fluorescent lipophilic tracers that successfully stain mammalian spermatozoa either did not stain fowl or turkey …

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Safety Of Brucella Abortus Strain Rb51 Vaccine In Non-Target Ungulates And Coyotes, Terry J. Kreeger, Thomas J. Deliberto, Steven C. Olsen, William H. Edwards, Walter E. Cook Jan 2002

Safety Of Brucella Abortus Strain Rb51 Vaccine In Non-Target Ungulates And Coyotes, Terry J. Kreeger, Thomas J. Deliberto, Steven C. Olsen, William H. Edwards, Walter E. Cook

United States Department of Agriculture Wildlife Services: Staff Publications

Brucellosis is endemic in free-ranging elk (Cervus elaphus) and bison (Bison bison) in the Greater Yellowstone Area (GYA; USA). It is possible that an oral brucellosis vaccine could be developed and disseminated in the GYA to reduce disease transmission. Should this occur, non-target species other than elk and bison may come in contact with the vaccine resulting in morbidity or mortality. To assess biosafety, bighorn sheep (Ovis canadensis; n=10), pronghorn (Antilocapra americana; n=9), mule deer (Odocoileus hemionus; n=11), moose (Alces alces shirasi; n=10), and …

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Corn Yield Potential And Optimal Soil Productivity In Irrigated Corn/Soybean Systems, Achim R. Dobermann, Timothy J. Arkebauer, Kenneth G. Cassman, Rhae A. Drijber, J. Lindquist, S. Madhaven, John P. Markwell, Lenis Alton Nelson, James E. Specht, Daniel T. Walters, Haishun Yang, Brigid Amos, Darren L. Binder, C. Murphy, Gregory J. Teichmeier Jan 2002

Corn Yield Potential And Optimal Soil Productivity In Irrigated Corn/Soybean Systems, Achim R. Dobermann, Timothy J. Arkebauer, Kenneth G. Cassman, Rhae A. Drijber, J. Lindquist, S. Madhaven, John P. Markwell, Lenis Alton Nelson, James E. Specht, Daniel T. Walters, Haishun Yang, Brigid Amos, Darren L. Binder, C. Murphy, Gregory J. Teichmeier

Department of Agronomy and Horticulture: Faculty Publications

In 1999, an interdisciplinary research team at the University of Nebraska established a field experiment to (1) quantify and understand the yield potential of corn and soybean under irrigated conditions, (2) identify efficient crop management practices to achieve yields that approach potential levels, and (3) determine the energy use efficiency, global warming and soil C-sequestration potential of intensively managed corn systems. The experiment compares systems that represent different levels of management intensity expressed as combinations of crop rotation (continuous corn, corn-soybean), plant density (low, medium, high) and nutrient management (recommended best management vs. intensive management). Detailed measurements include soil nutrient …

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Crop Sequence And Nitrogen Fertilization Effects On Soil Properties In The Western Corn Belt, M. A. Liebig, Gary E. Varvel, John W. Doran, Brian J. Wienhold Jan 2002

Crop Sequence And Nitrogen Fertilization Effects On Soil Properties In The Western Corn Belt, M. A. Liebig, Gary E. Varvel, John W. Doran, Brian J. Wienhold

Department of Agronomy and Horticulture: Faculty Publications

Understanding long-term management effects on soil properties is necessary to determine the relative sustainability of cropping systems. Soil physical, chemical, and biological properties were measured in a long-term cropping system study in the Western Corn Belt. Properties were evaluated after 16 yr in four crop sequences [continuous corn (Zea mays L.) (CC), corn–soybean [Glycine max. (L.)] (C–SB), corn–oat (Avena sativa L.) + clover (80% sweet clover [Melilotus officinalis L.] and 20% red clover [Trifolium pratense L.])–grain sorghum [(Sorghum bicolor (L.) Moench)–soybean (C–OCL–SG– SB), and corn–soybean-grain sorghum–oat + clover (C–SB–SG– OCL)] each at three …

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