Digital Commons Network^™

The Rna Binding Domain Of The Hantaan Virus N Protein Maps To A Central, Conserved Region, Colleen Jonsson, Xiaolin Xu, William Severson, Noah Villegas, Connie Schmaljohn

United States Army: Publications

The nucleocapsid (N) protein of hantaviruses encapsidates both viral genomic and antigenomic RNAs, although only the genomic viral RNA (vRNA) is packaged into virions. To define the domain within the Hantaan virus (HTNV) N protein that mediates these interactions, 14 N- and C-terminal deletion constructs were cloned into a bacterial expression vector, expressed, and purified to homogeneity. Each protein was examined for its ability to bind the HTNV S segment vRNA with filter binding and gel electrophoretic mobility shift assays. These studies mapped a minimal region within the HTNV N protein (amino acids 175 to 217) that bound vRNA. Sequence …

Invisible, Underserved, And Diverse: The Health Of Women In Prison, Janette Y. Taylor, Rachel Williams, M. J. Eliason

International Journal of Global Health

In the United States of America, women are the fastest growing segment of the criminal justice system. They are entering the system with far greater physical and mental health problems than men, but with fewer health services. Additionally, within this expanding population of incarcerated women, are disproportionately represented poor women of color with serious health needs. This article: a) uses an ecosocial model to examine and critique the health and healthcare of women in prison, b) examines social structures that influence incarceration and health status, and c) proposes reconsideration of current prison health services and education.

Suffolk Journal, Vol. 62, No. 3, 11/13/2002, Suffolk Journal

Suffolk Journal

No abstract provided.

2002 Stethoscope, University Of Health Sciences College Of Osteopathic Medicine

Yearbooks (1927-2005)

Annual publication of the University of Health Sciences College of Osteopathic Medicine.

Print copy located in the Special Collections room in the D'Angelo Library.

Characterization Of Cd4-Induced Epitopes On The Hiv Type 1 Gp120 Envelope Glycoprotein Recognized By Neutralizing Human Monoclonal Antibodies, Shi-Hua Xiang, Najah Doka, Rabeéa K. Choudhary, Joseph Sodroski, James E. Robinson

Nebraska Center for Virology: Faculty Publications

The entry of human immunodeficiency virus (HIV-1) into target cells typically requires the sequential binding of the viral exterior envelope glycoprotein, gp120, to CD4 and a chemokine receptor. CD4 binding exposes gp120 epitopes recognized by CD4-induced (CD4i) antibodies, which can block virus binding to the chemokine receptor. We identified three new CD4i antibodies from an HIV-1-infected individual and localized their epitopes. These epitopes include a highly conserved gp120 β-strand encompassing residues 419–424, which is also important for binding to the CCR5 chemokine receptor. All of the CD4i antibodies inhibited the binding of gp120–CD4 complexes to CCR5. CD4i antibodies and …

The Role Of Suggestions And Personality Characteristics In Producing Illness Reports And Desires For Suing The Responsible Party, Marc A. Lindberg Ph.D.

Psychology Faculty Research

For this project, 92 students entered an abandoned theater room in an old basement of the university where sand was scattered throughout. The purpose of the study was to experimentally demonstrate that psychological suggestions could produce illness reports and to explore who is most likely to say that they would sue for personal damages. The students filled out the Trait-State Anger Scale and two subscales, Anger Temperament and Anger Reaction as well as the Costello-Corey Anxiety Scale, the Hardiness Inventory, the Pennebaker Inventory of Limbic Languidness, and, embedded in the Hardiness Inventory, measures of current illness as a result of …

Moisture & Bugs, Leona K. Hawks, Andria B. Hansen

All Archived Publications

No abstract provided.

Bacterial Infectious Disease Control By Vaccine Development, Roy Curtiss Iii

Open Access Publications

No abstract provided.

Immune Responses To Recombinant Pneumococcal Pspa Antigen Delivered By Live Attenuated Salmonella Enterica Serovar Typhimurium Vaccine, Ho Young Kang, Jay Srinivasan, Roy Curtiss Iii

Open Access Publications

No abstract provided.

No. 24: Spaces Of Vulnerability: Migration And Hiv/Aids In South Africa, Brian Williams, Eleanor Gouws, Mark Lurie, Jonathan Crush

Southern African Migration Programme

Seventy per cent of the 36 million people infected worldwide with HIV live in Sub-Saharan Africa and within this region the countries of Southern Africa are the worst affected. The eight countries with the highest rates of infection are in Southern Africa, followed by six countries in East Africa, and then five other countries, only one outside Africa. The reasons why the highest rates of infection in the world occur in Southern Africa are unclear. Although the countries of the region have much in common, their histories over the last twenty years have been very different.

A number of different …

The Genome Of Swinepox Virus, C. L. Afonso, E. R. Tulman, Z. Lu, L. Zsak, Fernando A. Osorio, C. Balinsky, G. F. Kutish, D. L. Rock

Nebraska Center for Virology: Faculty Publications

Swinepox virus (SWPV), the sole member of the Suipoxvirus genus of the Poxviridae, is the etiologic agent of a worldwide disease specific for swine. Here we report the genomic sequence of SWPV. The 146-kbp SWPV genome consists of a central coding region bounded by identical 3.7-kbp inverted terminal repeats and contains 150 putative genes. Comparison of SWPV with chordopoxviruses reveals 146 conserved genes encoding proteins involved in basic replicative functions, viral virulence, host range, and immune evasion. Notably, these include genes with similarity to genes for gamma interferon (IFN-γ) receptor, IFN resistance protein, interleukin-18 binding protein, IFN-α/β binding protein, …

Adobe Medicus 2002 1 January-February, Health Sciences Library And Informatics Center

adobe medicus

No abstract provided.

Active Surveillance For Scrapie By Third Eyelid Biopsy And Genetic Susceptibility Testing Of Flocks Of Sheep In Wyoming, Katherine I. O'Rourke, John V. Duncan, James R. Logan, Anne K. Anderson, Dianne K. Norden, Elizabeth S. Williams, Bret A. Combs, Robert H. Stobart, Gary E. Moss, Diane L. Sutton

Zoonotics and Wildlife Disease: Publications

Control of scrapie, an ovine transmissible spongiform encephalopathy or prion disorder, has been hampered by the lack of conventional antemortem diagnostic tests. Currently, scrapie is diagnosed by postmortem examination of the brain and lymphoid tissues for PrP^Sc, the protein marker for this group of disorders. For live, asymptomatic sheep, diagnosis using tonsil or third-eyelid lymphoid tissue biopsy and PrP^Sc assay has been described. To evaluate the feasibility and efficacy of third-eyelid testing for identification of infected flocks and individual infected sheep, 690 sheep from 22 flocks were sampled by third-eyelid lymphoid tissue biopsy and immunohistochemistry. Sheep were …

Comparison Of Histological Lesions And Immunohistochemical Staining Of Proteinase-Resistant Prion Protein In A Naturally Occurring Spongiform Encephalopathy Of Free-Ranging Mule Deer (Odocoileus Hemionus) With Those Of Chronic Wasting Disease Of Captive Mule Deer, T. R. Spraker, R. R. Zink, B. A. Cummings, M. A. Wild, M. W. Miller, Katherine I. O'Rourke

Zoonotics and Wildlife Disease: Publications

In this investigation, the nature and distribution of histologic lesions and immunohistochemical staining (IHC) of a proteinase-resistant prion protein were compared in free-ranging mule deer (Odocoileus hemionus) dying of a naturally occurring spongiform encephalopathy (SE) and captive mule deer dying of chronic wasting disease (CWD). Sixteen free-ranging deer with SE, 12 free-ranging deer without SE, and 10 captive deer with CWD were examined at necropsy. Tissue sections were stained with hematoxylin and eosin, and duplicate sections were stained with a monoclonal antibody (F89/160.1.5). Histological lesions in the freeranging deer with SE and captive deer with CWD were found …

Greenhouse Gas Emissions And Emissions Trading In North America: Kyoto Treaty And U.S. Initiatives, Dale E. Stephenson

Canada-United States Law Journal

greenhouse gas emissions

Office Of Research And Graduate Studies -- Annual Report 2001/2002

Office of Research and Economic Development: Publications

03/04 Nanotechnology
05/06 Research Highlights
07/08 Biomedical Research
09/10 Children, Families & Education
11/12 Initiatives
13/14 Genomics
15/16 Research Centers
17/18 Graduate Studies
19/20 Technology Transfer

When I came to the University of Nebraska–Lincoln last year, I was not only impressed by the faculty and administration, but inspired by the potential to move UNL research to a new level of excellence. In the past fiscal year, we’ve experienced exceptional growth. Our total sponsored programs awards grew 25 percent, which is a direct result of our strategic initiatives that provide new resources, leverage existing resources, develop grantsmanship skills and increase collaborations …

Department Of Veterinary And Biomedical Sciences: 2002 Annual Report

School of Veterinary and Biomedical Sciences: Information and History

Foreword 2002
VBMS Faculty
VBMS Faculty and Staff, by Function and Unit
Honors. Awards and Recognitions. 2002
VBMS Committee Assignments. 2002
Faculty Profiles
VBMS Research Associates Profiles
VBMS Teaching Program
Great Plains Veterinary Educational Center (GPVEC)
VBMS Research Program
International Activities
Veterinary Extension Program
Nebraska Veterinary Diagnostic Laboratory Systems (NVDLS)
VBMS Grants and Contracts Program
Patents by VBMS Faculty in 2002
Publications by VBMS Faculty
Presentations by VBMS Faculty
Articles Regarding the Department
Selected Committees. Editorial and Other Appointments
Departmental Budget Summaries
Nebraska Agricultural Statistics

Short Report: Hepatitis B Infection And Severe Plasmodium Falciparum Malaria In Vietnamese Adults

United States Public Health Resources

We investigated the prevalence of infection with hepatitis B virus among adult Vietnamese patients hospitalized for severe Plasmodium falciparum malaria. Sera from patients admitted with severe malaria in Ho Chi Minh City, Vietnam, between May 1991 and January 1996 were assayed for hepatitis B surface antigen (HBsAg) by a commercial enzyme-linked immunosorbent assay kit. The overall prevalence of HB_sAg was 23.77% (77 of 324). This was higher than reported estimates of prevalence in the general catchment population for the study hospital (mean, 9.8%; range, 9–16%). No association was found between risk of death caused by severe malaria and …

Chloroquine For The Treatment Of Uncomplicated Malaria In Guyana, J. Kevin Baird, T. Tiwari, G. J. Martin, C. L. Tamminga, T. M. Prout, J. Tjaden, P. P. Bravet, S. Rawlins, M. Ferrel, D. Caruucci, S. L. Hoffman

United States Public Health Resources

At a public hospital in Georgetown, Guyana, 44 patients seeking treatment for symptomatic, slide-confirmed malaria were given standard chloroquine (CQ) therapy and followed for 28 days. The patients apparently had pure infections with Plasmodium falciparum (14), P. vivax (13) or P. malariae (one), or mixed infections either of P. falciparum and P. vivax (17) or of P. falciparum, P. malariae and P. vivax (two). Each received supervised treatment with 10 mg CQ base/kg on each of days 0 and 1, and 5 mg/kg on day 2. On the day of enrollment (day 0), the patients complained of fever (100%), …

Pathogenesis Of Human And Bovine Cryptosporidium Parvum In Gnotobiotic Pigs, Sonia J. Pereira, Norma E. Ramirez, Lihua Xiao, Lucy A. Ward

United States Public Health Resources

To compare the pathogenesis of human genotype 1 (HuGl) and bovine genotype 2 (BoG2) Cryptosporidium parvum, neonatal gnotobiotic pigs were given 1-10 HuGl or BoG2 oocysts. The prepatent and patent periods were significantly longer for HuGl than for BoG2 C. parvum (prepatent, 8.6 vs. 5.6 days; patent, 16.6 vs. 10.3 days). BoG2-infected pigs developed signif- icantly more severe disease than did HuGl-infected pigs. BoG2 parasites were seen micro- scopically throughout the intestines during the prepatent and patent periods. HuGl parasites were only detected during the patent period in the ileum and colon but colonized the mucosal surface in significantly …

A Preliminary Linkage Map Of The Tick, Ixodes Scapularis, Amy J. Ullmann, Joseph Piesman, M.C. Dolan, William C. Black, Iv

United States Public Health Resources

A linkage map of the Ixodes scapularis genome was constructed based upon segregation amongst 127 loci. These included 84 random amplified polymorphic DNA (RAPD) markers, 32 Sequence-Tagged RAPD (STAR) markers, 5 cDNAs, and 5 microsatellites in 232 F₁ intercross progeny from a single, field-collected P₁ female. A preliminary linkage map of 616 cM was generated across 14 linkage groups with one marker every 10.8 cM. Assuming a genome size of ∼10⁹ bp, the relationship of physical to genetic distance is ∼300 kb/cM in the I. scapularis genome.

Anopheles Gambiae Genome: Completing The Malaria Triad, Kathryn S. Aultman, Michael Gottlieb, Maria Y. Giovanni, Anthony S. Fauci

United States Public Health Resources

The genome sequence of Anopheles gambiae, the major mosquito vector of the malaria parasite Plasmodium falciparum, as well as analyses of its gene content and organization, are reported by several teams of investigators in this issue. In addition, the sequence of the P. falciparum genome appears in this week’s issue of Nature. The genomes of these two organisms, along with that of the human, provide a triad of critical genetic information relevant to all stages of the malaria transmission cycle and offer unprecedented opportunities to the scientific and public health communities engaged in the fight against malaria, …

Scwds Briefs: Volume 17, Number 4 (January 2002)

Southeastern Cooperative Wildlife Disease Study: Publications

• CWD News from Nebraska and Kansas
• Tropical Bont Tick Threat: Amblyomma variegatum
• Avian vacuolar myelinopathy (AVM) was confirmed by SCWDS diagnosticians in two dead bald eagles and is suspected in another four decomposed eagle carcasses recovered since mid-November at Clarks Hill Lake along the Georgia/South Carolina border.
• The Office International des Epipoozties (OIE) agreed to restore the United Kingdom's Foot-and-Mouth Disease (FMD) free status without vaccination for the purposes of international trade.
• Raccoon Roundworms – Public Health Update: Baylisascaris procyonis
• CSF Control Targets Wild Boars: Classical swine fever (CSF), also called hog cholera, is …

Risk Factors For Drug-Resistant Mycobacterium Tuberculosis In Saudi Arabia, Abdulrahman A. Alrajhi, Shahab Abdulwahab, Edna Almodovar, Hail M. Al-Abdely

Neurosciences

OBJECTIVE: To identify rates of primary and secondary drug-resistant Mycobacterium tuberculosis and their risk factors from a tertiary-care center in the Kingdom of Saudi Arabia. METHODS: Review of microbiological and clinical data of all patients with positive isolates of Mycobacterium tuberculosis between 1995 and 2000 at King Faisal Specialist Hospital and Research Centre, Riyadh, Kingdom of Saudi Arabia. RESULTS: Susceptibility to antituberculosis agents was tested in 320 isolates from 320 patients. The median age was 50 years. Pulmonary tuberculosis was diagnosed in 106 (33%) patients, extrapulmonary in 183 (57%), and both in 31 (10%) patients. Two hundred forty-six isolates were …

Bioterrorism And Medical Risk Management, Paul E. Pepe, Kathy J. Rinnert

The International Lawyer

No abstract provided.

International Health Law, Michele Forzley, Genevieve Grabman, Edmund G. Howe Iii

The International Lawyer

No abstract provided.

The Future Of Compulsory Licensing: Deciphering The Doha Declaration On The Trips Agreement And Public Health, Divya Murthy

American University International Law Review

No abstract provided.

The Utilization Of Services Of Health Care Providers Among Patients Presenting To Family Physicians At A Teaching Hospital In Karachi, Pakistan, Waris Qidwai, Iqbal Azam, S. S. Ali, S. Ayub

Department of Family Medicine

Objective: To study the utilization of services of Health care providers among patients presenting to Family Physicians in a teaching hospital in Karachi.
Methodology: It was a cross sectional study. A questionnaire was developed to collect patient’s utilization of services of health care providers. The investigators administered the questionnaire to 387 patients, after purpose of the study was explained, written consent was taken and confidentiality was assured.
Results: The study population included more females than males, with average age of 32.6 years. The majority were married, well educated, in private and government service, were students or housewives. …

Hiv/Aids Prevention Guidance For Reproductive Health Professionals In Developing-Country Settings, Helen Epstein, Daniel Whelan, Janneke Van De Wijgert, Purnima Mane, Suman Mehta

HIV and AIDS

While the integration of information, technologies, and services to respond to HIV/AIDS may seem closely related, there are a variety of issues that continue to create obstacles to integration. Among these are the stigmatizing nature of HIV infection and AIDS and discrimination faced by those who are infected or perceived to be infected; sexual practices and identities that remain socially unacceptable; gender roles and relations that make it difficult for women and men to access information, services, and technologies on HIV prevention; reluctance to recognize the special needs of young people; and barriers to service delivery created by broader economic, …

Cost Of Care And Support Services For Plha: Implications For The Financial Sustainability Of Nongovernmental Organizations, Horizons Program

HIV and AIDS

As HIV/AIDS prevalence increases, providers of care and support services will face greater demand for their services. The ability of nongovernmental organizations (NGOs) to help meet this increased demand will be influenced in part by their ability to control the cost of their services while generating sufficient revenue to meet expenses. The Y.R. Gaitonde Centre for AIDS Research and Education (YRG CARE) in Chennai, India, was one of the earliest providers of integrated care and support services in South India and currently serves clients from the four South Indian states. This brief highlights the key findings of a study that …