Digital Commons Network^™

Mutagenic Stabilization And/Or Disruption Of A Cd4-Bound State Reveals Distinct Conformations Of The Human Immunodeficiency Virus Type 1 Gp120 Envelope Glycoprotein, Shi-Hua Xiang, Peter D. Kwong, Rishi Gupta, Carlo D. Rizzuto, David J. Casper, Richard Wyatt, Liping Wang, Wayne A. Hendrickson, Michael L. Doyle, Joseph Sodroski

Nebraska Center for Virology: Faculty Publications

The human immunodeficiency virus type 1 (HIV-1) gp120 exterior envelope glycoprotein is conformationally flexible. Upon binding to the host cell receptor CD4, gp120 assumes a conformation that is recognized by the second receptor, CCR5 and/or CXCR4, and by the CD4-induced (CD4i) antibodies. Guided by the X-ray crystal structure of a gp120-CD4-CD4i antibody complex, we introduced changes into gp120 that were designed to stabilize or disrupt this conformation. One mutant, 375 S/W, in which the tryptophan indole group is predicted to occupy the Phe 43 cavity in the gp120 interior, apparently favors a gp120 conformation closer to that of the CD4-bound …

Human Immunodeficiency Virus Type 1 (Hiv-1) Tat Induces Nitric-Oxide Synthase In Human Astroglia, Xiaojuan Liu, Malabendu Jana, Subhajit Dasgupta, Sreenivas Koka, Jun He, Charles Wood, Kalipada Pahan

Nebraska Center for Virology: Faculty Publications

Human immunodeficiency virus type 1 (HIV-1) infection is known to cause neuronal injury and dementia in a significant proportion of patients. However, the mechanism by which HIV-1 mediates its deleterious effects in the brain is poorly defined. The present study was undertaken to investigate the effect of the HIV-1 tat gene on the expression of inducible nitric-oxide synthase (iNOS) in human U373MG astroglial cells and primary astroglia. Expression of the tat gene as RSV- tat but not that of the CAT gene as RSV-CAT in U373MG astroglial cells led to the induction of NO production and the expression of iNOS …

On A Collision Course--Two Potential Environmental Conflicts Between The U.S. And Canada, Frank E. Loy

Canada-United States Law Journal

environmental law--Canada, environmental law--United States

Canada-U.S. Environmental Cooperation, Alan Nymark

Canada-United States Law Journal

environmental law--Canada, environmental law--United States

Greenhouse Gas Emissions And Emissions Trading In North America: Kyoto Treaty And U.S. Initiatives - Introduction, Jonathan Adler

Canada-United States Law Journal

greenhouse gas emissions

Fresh Water: Environment Or Trade, Sanford E. Gaines

Canada-United States Law Journal

fresh water and trade, fresh water and environment

The Press Looks At The Problem Of Reconciling The Meeting Of Energy Demands With Environmental Protection: Background On The Issues, Peter Janson

Canada-United States Law Journal

energy and environment--Canada, energy and environment--United States

Discussion Following The Remarks Of Ms. Askari And Dr. Waddell, Discussion

Canada-United States Law Journal

energy and environment--Canada, energy and environment--United States

The Environmental Implications Of The Discovery And Delivery Of New Energy Resources In The Canadian Context, David Luff

Canada-United States Law Journal

new energy resources--Canada, alternative energy resources--United States

Discussion Following The Remarks Of Dr. Jhirad, Discussion

Canada-United States Law Journal

energy policy--21st century

0502-1 2002 Ballot Information Booklet, Colorado Legislative Council

All Publications (Colorado Legislative Council)

No abstract provided.

0502-4 2002 Ballot Information Booklet, Colorado Legislative Council

All Publications (Colorado Legislative Council)

No abstract provided.

0502-9 2002 Ballot Information Booklet, Colorado Legislative Council

All Publications (Colorado Legislative Council)

No abstract provided.

Masthead, Volume 12

Health Matrix: The Journal of Law-Medicine

No abstract provided.

University Report 2001-2002, Various

General Fiscal Chronicle

Annual Report to the Board Of Trustees of George Fox College

Annual Report Of The Officers For The Town Of Lincoln, N.H., Year Ending December 31, 2001., Lincoln Town Representatives

Lincoln, NH Annual Reports

This is an annual report containing vital statistics for a town/city in the state of New Hampshire.

Contempt Proceedings: Who Has The Onus For Investigating And Initiating The Charges Of Contempt – The Prosecutor, The Judge Or A Third Party?, Jennifer M. Wetmore

War Crimes Memoranda

No abstract provided.

Material Facts Not Pleaded In The Indictment, Jessica A. A. Levenberg

War Crimes Memoranda

No abstract provided.

Sentencing: An Assessment Of Rule 101 Of The Ictr Rules Of Procedure And Evidence, Federal Sentencing Laws In England, France And Other Countries As Well As In Prisoner-Receiving Countries, And The Kayishema/Ruzindana Judgement., Dora Kripapuri

War Crimes Memoranda

No abstract provided.

Whether A Guilty Plea Is A Mitigating Factor For Sentencing Purposes, And If So, Is It Mandatory Or Permissive?: A Critique Of The Kambanda Decision And A Comparative Study Of Plea Bargaining And Sentencing Issues In Ictr And Icty Jurisprudence And Other Common Law Countries, Amar Khoday

War Crimes Memoranda

No abstract provided.

Benjamin N. Cardozo: New York Giant, Robert M. Jarvis, Phyllis Coleman

Marquette Sports Law Review

No abstract provided.

The New Thought Police: Inside The Left’S Assault On Free Speech And Free Minds (Book Review), John W. Teeter Jr

Faculty Articles

Attacks on political correctness have grown both plentiful and rather tiresome. Such tomes occasionally score valid ideological points, but one grows weary of the bitter repetitiveness of it all. The New Thought Police might seem to offer a little novelty to the litany. Bruce is undeniably bright, impassioned, and edgy. Her book, however, is decidedly a mixed bag. The best parts center on her controversial role as a feminist spokeswoman during the O.J. Simpson murder trial. Bruce cogently emphasized that the case was a tragic paradigm of domestic violence rather than a racist conspiracy against a black cultural icon.

Bruce’s …

2002 Eastern Washington University Volleyball Program, Eastern Washington University. Associated Students

EWU Volleyball Programs

The program for the 2002 EWU volleyball season. The Eagles finished 29-2 that year and were coached by Wade Benson.

Animal Science Alumni Newsletter, Winter 2002

Department of Animal Science: Departmental News

Contents:

From the Department Head’s Desk
Faculty Retirement
New Faculty
100 Years of 4-H in Nebraska
Feedlot Internship Program and Feedlot Extension Meetings
Japanese Agricultural Training Program
Graduate Program
Animal Science Student Ambassadors
Bud Britton Animal Science Golf Classic
Masters Week
Deaths
Emeriti/Retiree Reception
Emeriti Update
Block & Bridle Activities
Animal Science Graduate Association
Recent Department Awards and Recognitions
CASNR Alumni Board
Academic Quadrathlon
Judging Team Activities
Attention — Judging Team Alumni
Alumni News
Alumni Newsletter Wins Award
Your Newsletter Committee
Call for Information for the 2003 Issue of the Animal Science Alumni Newsletter
Animal Science Trivia
Calendar of Events …

The Ogoni Of Nigeria, A. Olu Oyinlade, Jeffery M. Vincent

Department of Anthropology: Faculty Publications

The Ogoni are a minority ethnic people who live in the Western Niger Delta Region of southern Nigeria. During the 1970s, Ogoniland, or the Ogoni Nation, became part of the Rivers State of Nigeria. There are approximately 500,000 Ogoni who represent less than 0.05 percent of Nigeria's 100 to 120 million people. The population density of this region equals 1,233 people per square mile, making it one of the most densely populated areas of Nigeria. Reliable information about the origin of the Ogoni is limited. Archaeological and oral historical evidence suggests that the Ogoni have inhabited the area for over …

Uncoupling Proteins And Energy Expenditure In Mice Divergently Selected For Heat Loss, T. G. Mcdaneld, Merlyn K. Nielsen, Jess Miner

Department of Animal Science: Faculty Publications

The objective of this study was to determine whether variation in energy expenditure created by selection on heat loss is mediated by uncoupling protein-1 (UCP1) in brown adipose tissue. Divergent selection for heat loss developed lines of mice with high (MH) and low (ML) maintenance energy expenditure. Concentration of UCP1 mRNA in brown adipose tissue (BAT) was 93% greater in ML than in MH mice (P < 0.02). Two new lines of mice, KH and KL, were bred by backcrossing a UCP1 knockout gene into the MH and ML lines, respectively; KH and KL with both knockout (−/−) and wild type (+/+) UCP1 genotypes were generated. At 13 wk of age, KH mice exhibited greater heat loss (166 kcal∙kg^0.75∙d⁻¹) than KL mice (126.4 kcal∙ kg^0.75∙d⁻¹) regardless of the UCP1 knockout (P < 0.0001). Concentration of UCP2 mRNA in BAT was 74% greater in UCP1 knockout mice (−/−) than in wild type (+/+; P = 0.0001). We conclude that response to selection for increased energy expenditure was not mediated by increased expression or function of UCP1.

Effect Of Corn Processing On Starch Digestion And Bacterial Crude Protein Flow In Finishing Cattle, R. J. Cooper, C. T. Milton, Terry J. Klopfenstein, T. L. Scott, C. B. Wilson, R. A. Mass

Department of Animal Science: Faculty Publications

Six ruminally and duodenally cannulated yearling steers (523 kg) were used in a replicated 3 × 3 Latin square design experiment to study the effects of corn processing on nutrient digestion, bacterial CP production, and ruminal fermentation. Dietary treatments consisted of 90% concentrate diets that were based on dry-rolled (DRC), high-moisture (HMC), or steam-flaked (SFC) corn. Each diet contained 2.0% urea (DM basis) as the sole source of supplemental nitrogen. Each period lasted 17 d, with d 1 through 14 for diet adaptation and d 15 through 17 for fecal, duodenal, and ruminal sampling. Dry matter and OM intakes were …

Composition And Morphology Of The Follicular Basal Lamina During Atresia Of Bovine Antral Follicles, H. F. Irving-Rodgers, M. L. Mussard, J. E. Kinder, R. J. Rodgers

Department of Animal Science: Faculty Publications

The fate of the follicular basal lamina during atresia was investigated using bovine follicles, in which different follicle phenotypes have been observed. These phenotypes include: healthy follicles with rounded basal granulosa cells with an aligned basal lamina or follicles with columnar basal granulosa cells with a basal lamina of many loops (loopy), and atretic follicles in which either the antral granulosa cells (antral atresia) or the basal cells (basal atresia) die first. Loopy lamina and basal atresia occur only in small antral follicles < 5 mm in diameter. Follicles were collected from cattle of unknown reproductive history and processed for immunohistochemistry and electron microscopy, and from animals in which follicle growth had been monitored by daily measurements of follicle diameter by ultrasonography. Electron microscopic observations of dominant follicles during the growth phase, plateau and regression showed that the basal lamina was still visible and intact upon atresia. These follicles had a conventional aligned basal lamina, which they retained, except for some degree of folding, as they progressed into antral atresia. In small follicles (2–5 mm in diameter), the basal cell shape (rounded or columnar) and appearance of the basal lamina (aligned or of many loops) did not appear to be related to the type of atresia. On atresia the follicular basal laminae retained immunoreactive laminin α1 and β2, type IV collagen a1 and nidogen. Laminin α2, which may come from the theca, was present in the follicular basal lamina of only 22% of healthy follicles, but was expressed very commonly in 71% of the atretic follicles. Laminin α2 expression was found in both phenotypes of healthy follicles, antral and basal atretic follicles, and follicles with aligned or loopy basal laminae. It is concluded that the basal lamina is not degraded upon atresia, but does undergo a variety of other changes.

The Use Of X-Ray Computer Tomography For Measuring The Muscularity Of Live Sheep, H. E. Jones, R. M. Lewis, M. J. Young, B. T. Wolf

Department of Animal Science: Faculty Publications

Potential measures of muscularity derived from X-ray computer tomography (CT) were assessed using data for 160 sheep (50 Suffolk males, 50 Suffolk females, 40 Texel males and 20 Charollais males). One-fifth of the lambs within each breed and sex were slaughtered at each of 14,18 or 22 weeks ofage and two-fifths slaughtered at 26 weeks. All lambs were CT scanned prior to slaughter with longitudinal and cross-sectional scans taken at three positions along the body LSth lumbar vertebra (LV5), mid-shaft of the femur (FEM) and ischium USC)]. After slaughter, linear measurements of side length (SL) and M. longissimus thoracis et …

A Description Of The Growth Of Sheep And Its Genetic Analysis, R. M. Lewis, G. C. Emmans, G. Simm

Department of Animal Science: Faculty Publications

The Gompertz is one of a family of growth functions that, when the environment [eg.food, housing) is nonlimiting, provides a useful description of growth as a comparatively simple, single equation. It has three parameters of which the important ones are mature size, A, and the rate parameter, B. Estimates of A and B, however, are highly correlated and defining their separate values for individual animals is problematic. This problem was explored using five methods for estimating the parometers, or transformations ofthem, to describe the growth oftwo genotypes of Suffolk sheep kept under non-limiting conditions. 0 ne genotype was under selection …